MADD cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	MADD
Entrez Gene ID	101117276
Full Name	MAP kinase activating death domain
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012116765.1	XP_011972155.1	MAP kinase-activating death domain protein isoform X2
XM_012116770.1	XP_011972160.1	MAP kinase-activating death domain protein isoform X3
XM_012116775.1	XP_011972165.1	MAP kinase-activating death domain protein isoform X7
XM_012116776.1	XP_011972166.1	MAP kinase-activating death domain protein isoform X8
XM_012116779.1	XP_011972169.1	MAP kinase-activating death domain protein isoform X10
XM_012116790.1	XP_011972180.1	MAP kinase-activating death domain protein isoform X20
XM_027979735.1	XP_027835536.1	MAP kinase-activating death domain protein isoform X12
XM_027979739.1	XP_027835540.1	MAP kinase-activating death domain protein isoform X16
XM_027979746.1	XP_027835547.1	MAP kinase-activating death domain protein isoform X23
XM_027979747.1	XP_027835548.1	MAP kinase-activating death domain protein isoform X24
XM_012096378.2	XP_011951768.2	LOW QUALITY PROTEIN: MAP kinase-activating death domain protein isoform X1
XM_012116766.2	XP_011972156.1	MAP kinase-activating death domain protein isoform X2
XM_012116777.1	XP_011972167.1	MAP kinase-activating death domain protein isoform X9
XM_027979722.1	XP_027835523.1	MAP kinase-activating death domain protein isoform X1
XM_027979726.1	XP_027835527.1	MAP kinase-activating death domain protein isoform X3
XM_027979729.1	XP_027835530.1	MAP kinase-activating death domain protein isoform X6
XM_027979732.1	XP_027835533.1	MAP kinase-activating death domain protein isoform X9
XM_012116767.1	XP_011972157.1	MAP kinase-activating death domain protein isoform X2
XM_012116772.1	XP_011972162.1	MAP kinase-activating death domain protein isoform X4
XM_012116774.1	XP_011972164.1	MAP kinase-activating death domain protein isoform X6
XM_012116781.2	XP_011972171.1	MAP kinase-activating death domain protein isoform X12
XM_012116783.2	XP_011972173.1	MAP kinase-activating death domain protein isoform X14
XM_012116785.2	XP_011972175.1	MAP kinase-activating death domain protein isoform X16
XM_027979723.1	XP_027835524.1	MAP kinase-activating death domain protein isoform X1
XM_027979738.1	XP_027835539.1	MAP kinase-activating death domain protein isoform X15
XM_027979744.1	XP_027835545.1	MAP kinase-activating death domain protein isoform X21
XM_012116769.1	XP_011972159.1	MAP kinase-activating death domain protein isoform X2
XM_012116780.1	XP_011972170.1	MAP kinase-activating death domain protein isoform X11
XM_012116791.1	XP_011972181.1	MAP kinase-activating death domain protein isoform X21
XM_027979725.1	XP_027835526.1	MAP kinase-activating death domain protein isoform X2
XM_027979727.1	XP_027835528.1	MAP kinase-activating death domain protein isoform X4
XM_027979728.1	XP_027835529.1	MAP kinase-activating death domain protein isoform X5
XM_027979730.1	XP_027835531.1	MAP kinase-activating death domain protein isoform X7
XM_027979731.1	XP_027835532.1	MAP kinase-activating death domain protein isoform X8
XM_027979733.1	XP_027835534.1	MAP kinase-activating death domain protein isoform X10
XM_027979734.1	XP_027835535.1	MAP kinase-activating death domain protein isoform X11
XM_027979736.1	XP_027835537.1	MAP kinase-activating death domain protein isoform X13
XM_027979737.1	XP_027835538.1	MAP kinase-activating death domain protein isoform X14
XM_027979740.1	XP_027835541.1	MAP kinase-activating death domain protein isoform X17
XM_027979741.1	XP_027835542.1	MAP kinase-activating death domain protein isoform X18
XM_027979743.1	XP_027835544.1	MAP kinase-activating death domain protein isoform X20
XM_027979745.1	XP_027835546.1	MAP kinase-activating death domain protein isoform X22
XM_027979748.1	XP_027835549.1	MAP kinase-activating death domain protein isoform X25
XM_012116773.1	XP_011972163.1	MAP kinase-activating death domain protein isoform X5
XM_012116782.2	XP_011972172.1	MAP kinase-activating death domain protein isoform X13
XM_012116784.2	XP_011972174.1	MAP kinase-activating death domain protein isoform X15
XM_012116786.2	XP_011972176.1	MAP kinase-activating death domain protein isoform X17
XM_012116787.1	XP_011972177.1	MAP kinase-activating death domain protein isoform X18
XM_012116788.1	XP_011972178.1	MAP kinase-activating death domain protein isoform X19
XM_012116792.1	XP_011972182.1	MAP kinase-activating death domain protein isoform X22
XM_027979721.1	XP_027835522.1	MAP kinase-activating death domain protein isoform X1
XM_027979724.1	XP_027835525.1	MAP kinase-activating death domain protein isoform X1
XM_027979742.1	XP_027835543.1	MAP kinase-activating death domain protein isoform X19

Pathways from BioSystems
Gene Ontology

The following MADD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MADD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv05208	XM_012116792.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OSm66092	XM_027979748.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OOv05188	XM_012116769.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv05188	XM_012116767.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv05188	XM_012116765.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv05188	XM_012116766.2 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv05189	XM_012116770.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv05190	XM_012116772.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv05191	XM_012116773.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv05192	XM_012116774.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv05193	XM_012116775.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv05194	XM_012116776.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05195	XM_012116777.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05196	XM_012116779.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05197	XM_012116780.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05198	XM_012116781.2 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05199	XM_012116782.2 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05200	XM_012116783.2 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05201	XM_012116784.2 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05202	XM_012116785.2 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05203	XM_012116786.2 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05204	XM_012116787.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05205	XM_012116788.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05206	XM_012116790.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv05207	XM_012116791.1 Latest version!	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OSm53307	XM_012096378.2 Latest version!	Ovis aries MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OSm66068	XM_027979722.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm66068	XM_027979724.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm66068	XM_027979723.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm66068	XM_027979721.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm66069	XM_027979725.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm66070	XM_027979726.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm66071	XM_027979727.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm66072	XM_027979728.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm66073	XM_027979729.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm66074	XM_027979730.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66075	XM_027979731.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66076	XM_027979732.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66077	XM_027979733.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66078	XM_027979734.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66079	XM_027979735.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66080	XM_027979736.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66081	XM_027979737.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66082	XM_027979738.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66083	XM_027979739.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66084	XM_027979740.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66085	XM_027979741.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66086	XM_027979742.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66087	XM_027979743.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66088	XM_027979744.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66089	XM_027979745.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66090	XM_027979746.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm66091	XM_027979747.1 Latest version!	Ovis aries MAP kinase activating death domain (MADD), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MADD ( XM_012116792.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972182.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OOv05208
Clone ID Related Accession (Same CDS sequence)	XM_012116792.1
Accession Version	XM_012116792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972182.1
CDS	273..4649
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFME

Target ORF information:

RefSeq Version	XM_012116792.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116792.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979748.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835549.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X28, mRNA.

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CloneID	OSm66092
Clone ID Related Accession (Same CDS sequence)	XM_027979748.1
Accession Version	XM_027979748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835549.1
CDS	214..4590
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFME

Target ORF information:

RefSeq Version	XM_027979748.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979748.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116765.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972155.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.

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CloneID	OOv05188
Clone ID Related Accession (Same CDS sequence)	XM_012116765.1 , XM_012116766.2 , XM_012116767.1 , XM_012116769.1
Accession Version	XM_012116765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972155.1
CDS	273..5411
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_012116765.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116765.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116766.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972156.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOv05188
Clone ID Related Accession (Same CDS sequence)	XM_012116765.1 , XM_012116766.2 , XM_012116767.1 , XM_012116769.1
Accession Version	XM_012116766.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012116766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972156.1
CDS	725..5863
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_012116766.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116766.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116767.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972157.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOv05188
Clone ID Related Accession (Same CDS sequence)	XM_012116765.1 , XM_012116766.2 , XM_012116767.1 , XM_012116769.1
Accession Version	XM_012116767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972157.1
CDS	319..5457
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_012116767.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116767.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116769.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972159.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.

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CloneID	OOv05188
Clone ID Related Accession (Same CDS sequence)	XM_012116765.1 , XM_012116766.2 , XM_012116767.1 , XM_012116769.1
Accession Version	XM_012116769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972159.1
CDS	1001..6139
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_012116769.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116769.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116770.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972160.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.

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CloneID	OOv05189
Clone ID Related Accession (Same CDS sequence)	XM_012116770.1
Accession Version	XM_012116770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGCGATGGC 
CCAGGCGGCG AGGGCAGCGC CCACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGTGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCTG AAGATGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGCACCTC TGCAGAGAGG TCAGGAGGAG CAGGAGCGTA 
GGGAGGACGA GAAGGGAGGC AGGAAAGCTA GCCGGTGCTG CCAGGGAGCA GGCCCCAGAG 
CAGCGGCTTA ATGCCGGCCC AGAGCAGCGC TGGTGCTCAG GGGTTCCTCC AGCTGGCTGG 
ACCGGACCAG GCAGAAGTCA GAAAGTCCTG GGGGCAGAGC GGCCCAGCCG TGCCCCCGCT 
CAGCTGTGCT CACGTAGCCC GCCTCGGTCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972160.1
CDS	273..5408
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_012116770.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116770.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGCGATGGC 
CCAGGCGGCG AGGGCAGCGC CCACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGTGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCTG AAGATGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGCACCTC TGCAGAGAGG TCAGGAGGAG CAGGAGCGTA 
GGGAGGACGA GAAGGGAGGC AGGAAAGCTA GCCGGTGCTG CCAGGGAGCA GGCCCCAGAG 
CAGCGGCTTA ATGCCGGCCC AGAGCAGCGC TGGTGCTCAG GGGTTCCTCC AGCTGGCTGG 
ACCGGACCAG GCAGAAGTCA GAAAGTCCTG GGGGCAGAGC GGCCCAGCCG TGCCCCCGCT 
CAGCTGTGCT CACGTAGCCC GCCTCGGTCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116772.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972162.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OOv05190
Clone ID Related Accession (Same CDS sequence)	XM_012116772.1
Accession Version	XM_012116772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGCGATGG CCCAGGCGGC 
GAGGGCAGCG CCCACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGTGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTAAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTCTTCT GAAGATGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GCCTGCTGGC CACACTCCTG CACAATCTCA TCTCGCACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGAGATCTTT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGCTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAACGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAACT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGTGAA GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGGTAA TTACACTATT 
TTGCTCTTAG GTCTGGACTC ACATGGCAGT AACTCAAACC TCGGAGCTCC AGAGGAGGGA 
CTAGAGACAG AGAAGCACCT CTGCAGAGAG GTCAGGAGGA GCAGGAGCGT AGGGAGGACG 
AGAAGGGAGG CAGGAAAGCT AGCCGGTGCT GCCAGGGAGC AGGCCCCAGA GCAGCGGCTT 
AATGCCGGCC CAGAGCAGCG CTGGTGCTCA GGGGTTCCTC CAGCTGGCTG GACCGGACCA 
GGCAGAAGTC AGAAAGTCCT GGGGGCAGAG CGGCCCAGCC GTGCCCCCGC TCAGCTGTGC 
TCACGTAGCC CGCCTCGGTC CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972162.1
CDS	273..5399
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_012116772.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116772.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGCGATGG CCCAGGCGGC 
GAGGGCAGCG CCCACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGTGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTAAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTCTTCT GAAGATGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GCCTGCTGGC CACACTCCTG CACAATCTCA TCTCGCACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGAGATCTTT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGCTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAACGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAACT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGTGAA GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGGTAA TTACACTATT 
TTGCTCTTAG GTCTGGACTC ACATGGCAGT AACTCAAACC TCGGAGCTCC AGAGGAGGGA 
CTAGAGACAG AGAAGCACCT CTGCAGAGAG GTCAGGAGGA GCAGGAGCGT AGGGAGGACG 
AGAAGGGAGG CAGGAAAGCT AGCCGGTGCT GCCAGGGAGC AGGCCCCAGA GCAGCGGCTT 
AATGCCGGCC CAGAGCAGCG CTGGTGCTCA GGGGTTCCTC CAGCTGGCTG GACCGGACCA 
GGCAGAAGTC AGAAAGTCCT GGGGGCAGAG CGGCCCAGCC GTGCCCCCGC TCAGCTGTGC 
TCACGTAGCC CGCCTCGGTC CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116773.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972163.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.

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CloneID	OOv05191
Clone ID Related Accession (Same CDS sequence)	XM_012116773.1
Accession Version	XM_012116773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGC CTGCTGGCCA CACTCCTGCA CAATCTCATC 
TCGCACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG AGATCTTTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGCTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAACGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAACTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGTGAAGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC 
GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGCACCTCT GCAGAGAGGT CAGGAGGAGC 
AGGAGCGTAG GGAGGACGAG AAGGGAGGCA GGAAAGCTAG CCGGTGCTGC CAGGGAGCAG 
GCCCCAGAGC AGCGGCTTAA TGCCGGCCCA GAGCAGCGCT GGTGCTCAGG GGTTCCTCCA 
GCTGGCTGGA CCGGACCAGG CAGAAGTCAG AAAGTCCTGG GGGCAGAGCG GCCCAGCCGT 
GCCCCCGCTC AGCTGTGCTC ACGTAGCCCG CCTCGGTCCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972163.1
CDS	273..5357
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_012116773.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116773.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGC CTGCTGGCCA CACTCCTGCA CAATCTCATC 
TCGCACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG AGATCTTTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGCTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAACGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAACTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGTGAAGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC 
GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGCACCTCT GCAGAGAGGT CAGGAGGAGC 
AGGAGCGTAG GGAGGACGAG AAGGGAGGCA GGAAAGCTAG CCGGTGCTGC CAGGGAGCAG 
GCCCCAGAGC AGCGGCTTAA TGCCGGCCCA GAGCAGCGCT GGTGCTCAGG GGTTCCTCCA 
GCTGGCTGGA CCGGACCAGG CAGAAGTCAG AAAGTCCTGG GGGCAGAGCG GCCCAGCCGT 
GCCCCCGCTC AGCTGTGCTC ACGTAGCCCG CCTCGGTCCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116774.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972164.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.

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CloneID	OOv05192
Clone ID Related Accession (Same CDS sequence)	XM_012116774.1
Accession Version	XM_012116774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972164.1
CDS	273..5351
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_012116774.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116774.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116775.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972165.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.

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CloneID	OOv05193
Clone ID Related Accession (Same CDS sequence)	XM_012116775.1
Accession Version	XM_012116775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGCACCTC TGCAGAGAGG TCAGGAGGAG CAGGAGCGTA 
GGGAGGACGA GAAGGGAGGC AGGAAAGCTA GCCGGTGCTG CCAGGGAGCA GGCCCCAGAG 
CAGCGGCTTA ATGCCGGCCC AGAGCAGCGC TGGTGCTCAG GGGTTCCTCC AGCTGGCTGG 
ACCGGACCAG GCAGAAGTCA GAAAGTCCTG GGGGCAGAGC GGCCCAGCCG TGCCCCCGCT 
CAGCTGTGCT CACGTAGCCC GCCTCGGTCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972165.1
CDS	273..5348
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_012116775.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116775.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGCACCTC TGCAGAGAGG TCAGGAGGAG CAGGAGCGTA 
GGGAGGACGA GAAGGGAGGC AGGAAAGCTA GCCGGTGCTG CCAGGGAGCA GGCCCCAGAG 
CAGCGGCTTA ATGCCGGCCC AGAGCAGCGC TGGTGCTCAG GGGTTCCTCC AGCTGGCTGG 
ACCGGACCAG GCAGAAGTCA GAAAGTCCTG GGGGCAGAGC GGCCCAGCCG TGCCCCCGCT 
CAGCTGTGCT CACGTAGCCC GCCTCGGTCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116776.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972166.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OOv05194
Clone ID Related Accession (Same CDS sequence)	XM_012116776.1
Accession Version	XM_012116776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGCA 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CGCCGATGGC CCACGAGATC 
TGCTACTCTG TGCTGTGTCT CTTCTCGTAT GTGGCTGCAG TCCGCAGCAG TGAGGAGGAC 
CTCCGGACCC CGCCCCGGCC CCTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972166.1
CDS	273..5225
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFAVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116776.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116776.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGCA 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CGCCGATGGC CCACGAGATC 
TGCTACTCTG TGCTGTGTCT CTTCTCGTAT GTGGCTGCAG TCCGCAGCAG TGAGGAGGAC 
CTCCGGACCC CGCCCCGGCC CCTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116777.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972167.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.

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CloneID	OOv05195
Clone ID Related Accession (Same CDS sequence)	XM_012116777.1
Accession Version	XM_012116777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CGATGGCCCA CGAGATCTGC 
TACTCTGTGC TGTGTCTCTT CTCGTATGTG GCTGCAGTCC GCAGCAGTGA GGAGGACCTC 
CGGACCCCGC CCCGGCCCCT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972167.1
CDS	273..5222
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116777.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116777.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CGATGGCCCA CGAGATCTGC 
TACTCTGTGC TGTGTCTCTT CTCGTATGTG GCTGCAGTCC GCAGCAGTGA GGAGGACCTC 
CGGACCCCGC CCCGGCCCCT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116779.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972169.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.

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CloneID	OOv05196
Clone ID Related Accession (Same CDS sequence)	XM_012116779.1
Accession Version	XM_012116779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGC AGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972169.1
CDS	273..5216
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFAVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116779.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116779.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGC AGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116780.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972170.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.

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CloneID	OOv05197
Clone ID Related Accession (Same CDS sequence)	XM_012116780.1
Accession Version	XM_012116780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCGATGGCCC ACGAGATCTG CTACTCTGTG 
CTGTGTCTCT TCTCGTATGT GGCTGCAGTC CGCAGCAGTG AGGAGGACCT CCGGACCCCG 
CCCCGGCCCC TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972170.1
CDS	273..5213
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116780.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116780.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCGATGGCCC ACGAGATCTG CTACTCTGTG 
CTGTGTCTCT TCTCGTATGT GGCTGCAGTC CGCAGCAGTG AGGAGGACCT CCGGACCCCG 
CCCCGGCCCC TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116781.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972171.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OOv05198
Clone ID Related Accession (Same CDS sequence)	XM_012116781.2
Accession Version	XM_012116781.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012116781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CGATGGCCCA CGAGATCTGC 
TACTCTGTGC TGTGTCTCTT CTCGTATGTG GCTGCAGTCC GCAGCAGTGA GGAGGACCTC 
CGGACCCCGC CCCGGCCCCT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972171.1
CDS	273..5162
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116781.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116781.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CGATGGCCCA CGAGATCTGC 
TACTCTGTGC TGTGTCTCTT CTCGTATGTG GCTGCAGTCC GCAGCAGTGA GGAGGACCTC 
CGGACCCCGC CCCGGCCCCT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116782.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972172.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OOv05199
Clone ID Related Accession (Same CDS sequence)	XM_012116782.2
Accession Version	XM_012116782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012116782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972172.1
CDS	273..5096
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116782.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116782.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116783.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972173.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.

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CloneID	OOv05200
Clone ID Related Accession (Same CDS sequence)	XM_012116783.2
Accession Version	XM_012116783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012116783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGCCT GCTGGCCACA CTCCTGCACA ATCTCATCTC GCACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGAG ATCTTTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG CTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAACGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAACTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGTGAAGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCGATG GCCCACGAGA TCTGCTACTC TGTGCTGTGT 
CTCTTCTCGT ATGTGGCTGC AGTCCGCAGC AGTGAGGAGG ACCTCCGGAC CCCGCCCCGG 
CCCCTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972173.1
CDS	273..5087
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116783.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116783.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGCCT GCTGGCCACA CTCCTGCACA ATCTCATCTC GCACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGAG ATCTTTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG CTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAACGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAACTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGTGAAGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCGATG GCCCACGAGA TCTGCTACTC TGTGCTGTGT 
CTCTTCTCGT ATGTGGCTGC AGTCCGCAGC AGTGAGGAGG ACCTCCGGAC CCCGCCCCGG 
CCCCTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116784.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972174.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.

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CloneID	OOv05201
Clone ID Related Accession (Same CDS sequence)	XM_012116784.2
Accession Version	XM_012116784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012116784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972174.1
CDS	273..5036
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116784.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116784.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116785.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972175.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.

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CloneID	OOv05202
Clone ID Related Accession (Same CDS sequence)	XM_012116785.2
Accession Version	XM_012116785.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012116785.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGC 
GATGGCCCAG GCGGCGAGGG CAGCGCCCAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGTGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCTGAAGA TGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCGATGGCCC ACGAGATCTG CTACTCTGTG 
CTGTGTCTCT TCTCGTATGT GGCTGCAGTC CGCAGCAGTG AGGAGGACCT CCGGACCCCG 
CCCCGGCCCC TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972175.1
CDS	273..5033
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116785.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116785.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGC 
GATGGCCCAG GCGGCGAGGG CAGCGCCCAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGTGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCTGAAGA TGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCGATGGCCC ACGAGATCTG CTACTCTGTG 
CTGTGTCTCT TCTCGTATGT GGCTGCAGTC CGCAGCAGTG AGGAGGACCT CCGGACCCCG 
CCCCGGCCCC TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116786.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972176.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.

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CloneID	OOv05203
Clone ID Related Accession (Same CDS sequence)	XM_012116786.2
Accession Version	XM_012116786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012116786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGCCTGCT GGCCACACTC CTGCACAATC TCATCTCGCA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGAGATC TTTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGCTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA CGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ACTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGT GAAGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCGATGGCC CACGAGATCT GCTACTCTGT GCTGTGTCTC 
TTCTCGTATG TGGCTGCAGT CCGCAGCAGT GAGGAGGACC TCCGGACCCC GCCCCGGCCC 
CTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972176.1
CDS	273..5024
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_012116786.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116786.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGCCTGCT GGCCACACTC CTGCACAATC TCATCTCGCA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGAGATC TTTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGCTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA CGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ACTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGT GAAGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCGATGGCC CACGAGATCT GCTACTCTGT GCTGTGTCTC 
TTCTCGTATG TGGCTGCAGT CCGCAGCAGT GAGGAGGACC TCCGGACCCC GCCCCGGCCC 
CTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116787.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972177.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OOv05204
Clone ID Related Accession (Same CDS sequence)	XM_012116787.1
Accession Version	XM_012116787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972177.1
CDS	273..5015
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_012116787.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116787.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116788.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972178.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OOv05205
Clone ID Related Accession (Same CDS sequence)	XM_012116788.1
Accession Version	XM_012116788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGCGATGGC 
CCAGGCGGCG AGGGCAGCGC CCACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGTGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCTG AAGATGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972178.1
CDS	273..5012
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_012116788.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116788.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGCGATGGC 
CCAGGCGGCG AGGGCAGCGC CCACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGTGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCTG AAGATGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116790.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972180.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.

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CloneID	OOv05206
Clone ID Related Accession (Same CDS sequence)	XM_012116790.1
Accession Version	XM_012116790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972180.1
CDS	273..4955
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_012116790.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116790.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCTG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012116791.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011972181.1 Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X26, mRNA.

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CloneID	OOv05207
Clone ID Related Accession (Same CDS sequence)	XM_012116791.1
Accession Version	XM_012116791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011972181.1
CDS	273..4838
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_012116791.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon MAP-kinase activating death domain (MADD), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012116791.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT GCCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCTGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_012096378.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011951768.2 Ovis aries MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm53307
Clone ID Related Accession (Same CDS sequence)	XM_012096378.2
Accession Version	XM_012096378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639024.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012096378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAATTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
TCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCAGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
NACCCTCGAA GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA 
CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011951768.2
CDS	3193..7938
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRSSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDVIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVnullPRRDQPQVHAQPGFHRAESH

Target ORF information:

RefSeq Version	XM_012096378.2
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012096378.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAATTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCCTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCATGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
TCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTGGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACGGTGCA GTCCGAGGAG GAGGCCCGGC AGGACGTCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA CGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCAGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
NACCCTCGAA GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA 
CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979722.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835523.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSm66068
Clone ID Related Accession (Same CDS sequence)	XM_027979722.1 , XM_027979723.1 , XM_027979721.1 , XM_027979724.1
Accession Version	XM_027979722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835523.1
CDS	704..5842
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_027979722.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979722.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979723.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835524.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

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CloneID	OSm66068
Clone ID Related Accession (Same CDS sequence)	XM_027979722.1 , XM_027979723.1 , XM_027979721.1 , XM_027979724.1
Accession Version	XM_027979723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835524.1
CDS	219..5357
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_027979723.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979723.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979721.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835522.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

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CloneID	OSm66068
Clone ID Related Accession (Same CDS sequence)	XM_027979722.1 , XM_027979723.1 , XM_027979721.1 , XM_027979724.1
Accession Version	XM_027979721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835522.1
CDS	212..5350
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_027979721.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979721.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979724.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835525.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

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CloneID	OSm66068
Clone ID Related Accession (Same CDS sequence)	XM_027979722.1 , XM_027979723.1 , XM_027979721.1 , XM_027979724.1
Accession Version	XM_027979724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835525.1
CDS	304..5442
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_027979724.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979724.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979725.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835526.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

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CloneID	OSm66069
Clone ID Related Accession (Same CDS sequence)	XM_027979725.1
Accession Version	XM_027979725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGCGATGGC 
CCAGGCGGCG AGGGCAGCGC CCACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGTGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCTG AAGATGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGCACCTC TGCAGAGAGG TCAGGAGGAG CAGGAGCGTA 
GGGAGGACGA GAAGGGAGGC AGGAAAGCTA GCCGGTGCTG CCAGGGAGCA GGCCCCAGAG 
CAGCGGCTTA ATGCCGGCCC AGAGCAGCGC TGGTGCTCAG GGGTTCCTCC AGCTGGCTGG 
ACCGGACCAG GCAGAAGTCA GAAAGTCCTG GGGGCAGAGC GGCCCAGCCG TGCCCCCGCT 
CAGCTGTGCT CACGTAGCCC GCCTCGGTCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835526.1
CDS	212..5347
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_027979725.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979725.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGCGATGGC 
CCAGGCGGCG AGGGCAGCGC CCACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGTGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCTG AAGATGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGCACCTC TGCAGAGAGG TCAGGAGGAG CAGGAGCGTA 
GGGAGGACGA GAAGGGAGGC AGGAAAGCTA GCCGGTGCTG CCAGGGAGCA GGCCCCAGAG 
CAGCGGCTTA ATGCCGGCCC AGAGCAGCGC TGGTGCTCAG GGGTTCCTCC AGCTGGCTGG 
ACCGGACCAG GCAGAAGTCA GAAAGTCCTG GGGGCAGAGC GGCCCAGCCG TGCCCCCGCT 
CAGCTGTGCT CACGTAGCCC GCCTCGGTCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979726.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835527.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

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CloneID	OSm66070
Clone ID Related Accession (Same CDS sequence)	XM_027979726.1
Accession Version	XM_027979726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGCGATGG CCCAGGCGGC 
GAGGGCAGCG CCCACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGTGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTAAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTCTTCT GAAGATGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GCCTGCTGGC CACACTCCTG CACAATCTCA TCTCGCACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGAGATCTTT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGCTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAACGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAACT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGTGAA GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGGTAA TTACACTATT 
TTGCTCTTAG GTCTGGACTC ACATGGCAGT AACTCAAACC TCGGAGCTCC AGAGGAGGGA 
CTAGAGACAG AGAAGCACCT CTGCAGAGAG GTCAGGAGGA GCAGGAGCGT AGGGAGGACG 
AGAAGGGAGG CAGGAAAGCT AGCCGGTGCT GCCAGGGAGC AGGCCCCAGA GCAGCGGCTT 
AATGCCGGCC CAGAGCAGCG CTGGTGCTCA GGGGTTCCTC CAGCTGGCTG GACCGGACCA 
GGCAGAAGTC AGAAAGTCCT GGGGGCAGAG CGGCCCAGCC GTGCCCCCGC TCAGCTGTGC 
TCACGTAGCC CGCCTCGGTC CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835527.1
CDS	212..5338
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_027979726.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979726.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGCGATGG CCCAGGCGGC 
GAGGGCAGCG CCCACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGTGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTAAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTCTTCT GAAGATGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GCCTGCTGGC CACACTCCTG CACAATCTCA TCTCGCACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGAGATCTTT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGCTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAACGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAACT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGTGAA GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGGTAA TTACACTATT 
TTGCTCTTAG GTCTGGACTC ACATGGCAGT AACTCAAACC TCGGAGCTCC AGAGGAGGGA 
CTAGAGACAG AGAAGCACCT CTGCAGAGAG GTCAGGAGGA GCAGGAGCGT AGGGAGGACG 
AGAAGGGAGG CAGGAAAGCT AGCCGGTGCT GCCAGGGAGC AGGCCCCAGA GCAGCGGCTT 
AATGCCGGCC CAGAGCAGCG CTGGTGCTCA GGGGTTCCTC CAGCTGGCTG GACCGGACCA 
GGCAGAAGTC AGAAAGTCCT GGGGGCAGAG CGGCCCAGCC GTGCCCCCGC TCAGCTGTGC 
TCACGTAGCC CGCCTCGGTC CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979727.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835528.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OSm66071
Clone ID Related Accession (Same CDS sequence)	XM_027979727.1
Accession Version	XM_027979727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGC CTGCTGGCCA CACTCCTGCA CAATCTCATC 
TCGCACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG AGATCTTTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGCTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAACGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAACTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGTGAAGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC 
GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGCACCTCT GCAGAGAGGT CAGGAGGAGC 
AGGAGCGTAG GGAGGACGAG AAGGGAGGCA GGAAAGCTAG CCGGTGCTGC CAGGGAGCAG 
GCCCCAGAGC AGCGGCTTAA TGCCGGCCCA GAGCAGCGCT GGTGCTCAGG GGTTCCTCCA 
GCTGGCTGGA CCGGACCAGG CAGAAGTCAG AAAGTCCTGG GGGCAGAGCG GCCCAGCCGT 
GCCCCCGCTC AGCTGTGCTC ACGTAGCCCG CCTCGGTCCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835528.1
CDS	211..5295
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_027979727.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979727.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGACG CTGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAGT TCTGGGAAGA CGCGTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGAATG 
GGCATGGACC AGGGTCCCCA GGAAATGATA GACAGGTACC TGTCCCTGGG AGAACACGAC 
CGGAAGCGCC TGGAGGACGA TGAAGACCGC CTGCTGGCCA CACTCCTGCA CAATCTCATC 
TCGCACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTG 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGTCAGCAAA TCAATGAAGT CCTTGACCAG 
CTGGCCAACC TGAATGGACG AGATCTTTCC ATCCGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TCGTGGTACA TGCAGGGACA GACACGAATG GAGATATCTT TTTCATGGAG 
GTGTGTGACG ACTGCGTGGT GCTGCGCAGT AACATCGGGA CGCTGTACGA GCGCTGGTGG 
TACGAGAAAC TCATCAACAT GACCTACTGC CCCAAGACCA AGGTGCTGTG CTTGTGGCGC 
AGAAACGGCT CCGAGACCCA GCTCAACAAG TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGCGTGA AGGACAGCAT GGAGCGCGCC GCCGCCCGCC AGCAGAGCAT CAAGCCGGGG 
CCGGAACTGG GTGGCGAGTT CCCCGTGCAG GACATGAAGA CTGGTGAAGG GGGCTTGCTG 
CAGGTCACCC TCGAAGGGAT CAACCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGGTAATT ACACTATTTT GCTCTTAGGT CTGGACTCAC ATGGCAGTAA CTCAAACCTC 
GGAGCTCCAG AGGAGGGACT AGAGACAGAG AAGCACCTCT GCAGAGAGGT CAGGAGGAGC 
AGGAGCGTAG GGAGGACGAG AAGGGAGGCA GGAAAGCTAG CCGGTGCTGC CAGGGAGCAG 
GCCCCAGAGC AGCGGCTTAA TGCCGGCCCA GAGCAGCGCT GGTGCTCAGG GGTTCCTCCA 
GCTGGCTGGA CCGGACCAGG CAGAAGTCAG AAAGTCCTGG GGGCAGAGCG GCCCAGCCGT 
GCCCCCGCTC AGCTGTGCTC ACGTAGCCCG CCTCGGTCCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979728.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835529.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

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CloneID	OSm66072
Clone ID Related Accession (Same CDS sequence)	XM_027979728.1
Accession Version	XM_027979728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835529.1
CDS	211..5289
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_027979728.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979728.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGGT 
AATTACACTA TTTTGCTCTT AGGTCTGGAC TCACATGGCA GTAACTCAAA CCTCGGAGCT 
CCAGAGGAGG GACTAGAGAC AGAGAAGCAC CTCTGCAGAG AGGTCAGGAG GAGCAGGAGC 
GTAGGGAGGA CGAGAAGGGA GGCAGGAAAG CTAGCCGGTG CTGCCAGGGA GCAGGCCCCA 
GAGCAGCGGC TTAATGCCGG CCCAGAGCAG CGCTGGTGCT CAGGGGTTCC TCCAGCTGGC 
TGGACCGGAC CAGGCAGAAG TCAGAAAGTC CTGGGGGCAG AGCGGCCCAG CCGTGCCCCC 
GCTCAGCTGT GCTCACGTAG CCCGCCTCGG TCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979729.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835530.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OSm66073
Clone ID Related Accession (Same CDS sequence)	XM_027979729.1
Accession Version	XM_027979729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGCACCTC TGCAGAGAGG TCAGGAGGAG CAGGAGCGTA 
GGGAGGACGA GAAGGGAGGC AGGAAAGCTA GCCGGTGCTG CCAGGGAGCA GGCCCCAGAG 
CAGCGGCTTA ATGCCGGCCC AGAGCAGCGC TGGTGCTCAG GGGTTCCTCC AGCTGGCTGG 
ACCGGACCAG GCAGAAGTCA GAAAGTCCTG GGGGCAGAGC GGCCCAGCCG TGCCCCCGCT 
CAGCTGTGCT CACGTAGCCC GCCTCGGTCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835530.1
CDS	211..5286
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFGNYTILLLGLDSHGSNSNLGAPEEGLETEKHLCREVRRSRSVGRTRREAGKLAGAAREQAPEQRLNAGPEQRWCSGVPPAGWTGPGRSQKVLGAERPSRAPAQLCSRSPPRSL

Target ORF information:

RefSeq Version	XM_027979729.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979729.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGGTAAT 
TACACTATTT TGCTCTTAGG TCTGGACTCA CATGGCAGTA ACTCAAACCT CGGAGCTCCA 
GAGGAGGGAC TAGAGACAGA GAAGCACCTC TGCAGAGAGG TCAGGAGGAG CAGGAGCGTA 
GGGAGGACGA GAAGGGAGGC AGGAAAGCTA GCCGGTGCTG CCAGGGAGCA GGCCCCAGAG 
CAGCGGCTTA ATGCCGGCCC AGAGCAGCGC TGGTGCTCAG GGGTTCCTCC AGCTGGCTGG 
ACCGGACCAG GCAGAAGTCA GAAAGTCCTG GGGGCAGAGC GGCCCAGCCG TGCCCCCGCT 
CAGCTGTGCT CACGTAGCCC GCCTCGGTCC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979730.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835531.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

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CloneID	OSm66074
Clone ID Related Accession (Same CDS sequence)	XM_027979730.1
Accession Version	XM_027979730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGCA 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CGCCGATGGC CCACGAGATC 
TGCTACTCTG TGCTGTGTCT CTTCTCGTAT GTGGCTGCAG TCCGCAGCAG TGAGGAGGAC 
CTCCGGACCC CGCCCCGGCC CCTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835531.1
CDS	210..5162
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFAVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979730.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979730.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGCA 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CGCCGATGGC CCACGAGATC 
TGCTACTCTG TGCTGTGTCT CTTCTCGTAT GTGGCTGCAG TCCGCAGCAG TGAGGAGGAC 
CTCCGGACCC CGCCCCGGCC CCTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979731.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835532.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

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CloneID	OSm66075
Clone ID Related Accession (Same CDS sequence)	XM_027979731.1
Accession Version	XM_027979731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CGATGGCCCA CGAGATCTGC 
TACTCTGTGC TGTGTCTCTT CTCGTATGTG GCTGCAGTCC GCAGCAGTGA GGAGGACCTC 
CGGACCCCGC CCCGGCCCCT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835532.1
CDS	209..5158
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979731.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979731.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CGATGGCCCA CGAGATCTGC 
TACTCTGTGC TGTGTCTCTT CTCGTATGTG GCTGCAGTCC GCAGCAGTGA GGAGGACCTC 
CGGACCCCGC CCCGGCCCCT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979732.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835533.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

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CloneID	OSm66076
Clone ID Related Accession (Same CDS sequence)	XM_027979732.1
Accession Version	XM_027979732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCGATGGCCC ACGAGATCTG CTACTCTGTG 
CTGTGTCTCT TCTCGTATGT GGCTGCAGTC CGCAGCAGTG AGGAGGACCT CCGGACCCCG 
CCCCGGCCCC TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835533.1
CDS	208..5148
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979732.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979732.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCGATGGCCC ACGAGATCTG CTACTCTGTG 
CTGTGTCTCT TCTCGTATGT GGCTGCAGTC CGCAGCAGTG AGGAGGACCT CCGGACCCCG 
CCCCGGCCCC TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979733.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835534.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

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CloneID	OSm66077
Clone ID Related Accession (Same CDS sequence)	XM_027979733.1
Accession Version	XM_027979733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGCGATGG CCCAGGCGGC 
GAGGGCAGCG CCCACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGTGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTAAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTCTTCT GAAGATGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GCCTGCTGGC CACACTCCTG CACAATCTCA TCTCGCACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGAGATCTTT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGCTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAACGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAACT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGTGAA GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC GATGGCCCAC GAGATCTGCT ACTCTGTGCT GTGTCTCTTC 
TCGTATGTGG CTGCAGTCCG CAGCAGTGAG GAGGACCTCC GGACCCCGCC CCGGCCCCTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835534.1
CDS	208..5136
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979733.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979733.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGCGATGG CCCAGGCGGC 
GAGGGCAGCG CCCACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGTGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTAAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTCTTCT GAAGATGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GCCTGCTGGC CACACTCCTG CACAATCTCA TCTCGCACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGAGATCTTT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGCTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAACGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAACT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGTGAA GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACGCC GATGGCCCAC GAGATCTGCT ACTCTGTGCT GTGTCTCTTC 
TCGTATGTGG CTGCAGTCCG CAGCAGTGAG GAGGACCTCC GGACCCCGCC CCGGCCCCTC 
TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979734.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835535.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OSm66078
Clone ID Related Accession (Same CDS sequence)	XM_027979734.1
Accession Version	XM_027979734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CGATGGCCCA CGAGATCTGC 
TACTCTGTGC TGTGTCTCTT CTCGTATGTG GCTGCAGTCC GCAGCAGTGA GGAGGACCTC 
CGGACCCCGC CCCGGCCCCT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835535.1
CDS	208..5097
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979734.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979734.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACGC CGATGGCCCA CGAGATCTGC 
TACTCTGTGC TGTGTCTCTT CTCGTATGTG GCTGCAGTCC GCAGCAGTGA GGAGGACCTC 
CGGACCCCGC CCCGGCCCCT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979735.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835536.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

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CloneID	OSm66079
Clone ID Related Accession (Same CDS sequence)	XM_027979735.1
Accession Version	XM_027979735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835536.1
CDS	207..5030
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979735.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979735.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979736.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835537.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

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CloneID	OSm66080
Clone ID Related Accession (Same CDS sequence)	XM_027979736.1
Accession Version	XM_027979736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGCCT GCTGGCCACA CTCCTGCACA ATCTCATCTC GCACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGAG ATCTTTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG CTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAACGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAACTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGTGAAGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCGATG GCCCACGAGA TCTGCTACTC TGTGCTGTGT 
CTCTTCTCGT ATGTGGCTGC AGTCCGCAGC AGTGAGGAGG ACCTCCGGAC CCCGCCCCGG 
CCCCTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835537.1
CDS	206..5020
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979736.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979736.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGCCT GCTGGCCACA CTCCTGCACA ATCTCATCTC GCACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGAG ATCTTTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG CTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAACGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAACTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGTGAAGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTCCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCGATG GCCCACGAGA TCTGCTACTC TGTGCTGTGT 
CTCTTCTCGT ATGTGGCTGC AGTCCGCAGC AGTGAGGAGG ACCTCCGGAC CCCGCCCCGG 
CCCCTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979737.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835538.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

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CloneID	OSm66081
Clone ID Related Accession (Same CDS sequence)	XM_027979737.1
Accession Version	XM_027979737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGCCTGCT GGCCACACTC CTGCACAATC TCATCTCGCA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGAGATC TTTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGCTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA CGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ACTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGT GAAGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG CAGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCGATG GCCCACGAGA TCTGCTACTC TGTGCTGTGT 
CTCTTCTCGT ATGTGGCTGC AGTCCGCAGC AGTGAGGAGG ACCTCCGGAC CCCGCCCCGG 
CCCCTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835538.1
CDS	207..5021
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFAVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979737.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979737.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGCCTGCT GGCCACACTC CTGCACAATC TCATCTCGCA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGAGATC TTTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGCTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA CGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ACTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGT GAAGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG CAGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACGCCGATG GCCCACGAGA TCTGCTACTC TGTGCTGTGT 
CTCTTCTCGT ATGTGGCTGC AGTCCGCAGC AGTGAGGAGG ACCTCCGGAC CCCGCCCCGG 
CCCCTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979738.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835539.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

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CloneID	OSm66082
Clone ID Related Accession (Same CDS sequence)	XM_027979738.1
Accession Version	XM_027979738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGCCTGCT GGCCACACTC CTGCACAATC TCATCTCGCA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGAGATC TTTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGCTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA CGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ACTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGT GAAGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCGATGGCC CACGAGATCT GCTACTCTGT GCTGTGTCTC 
TTCTCGTATG TGGCTGCAGT CCGCAGCAGT GAGGAGGACC TCCGGACCCC GCCCCGGCCC 
CTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835539.1
CDS	207..5018
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979738.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979738.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGCCTGCT GGCCACACTC CTGCACAATC TCATCTCGCA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGAGATC TTTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGCTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA CGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ACTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGT GAAGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCGATGGCC CACGAGATCT GCTACTCTGT GCTGTGTCTC 
TTCTCGTATG TGGCTGCAGT CCGCAGCAGT GAGGAGGACC TCCGGACCCC GCCCCGGCCC 
CTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979739.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835540.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OSm66083
Clone ID Related Accession (Same CDS sequence)	XM_027979739.1
Accession Version	XM_027979739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835540.1
CDS	206..4969
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979739.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979739.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACGCCGATGG CCCACGAGAT CTGCTACTCT 
GTGCTGTGTC TCTTCTCGTA TGTGGCTGCA GTCCGCAGCA GTGAGGAGGA CCTCCGGACC 
CCGCCCCGGC CCCTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979740.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835541.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OSm66084
Clone ID Related Accession (Same CDS sequence)	XM_027979740.1
Accession Version	XM_027979740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGC 
GATGGCCCAG GCGGCGAGGG CAGCGCCCAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGTGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCTGAAGA TGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCGATGGCCC ACGAGATCTG CTACTCTGTG 
CTGTGTCTCT TCTCGTATGT GGCTGCAGTC CGCAGCAGTG AGGAGGACCT CCGGACCCCG 
CCCCGGCCCC TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835541.1
CDS	206..4966
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979740.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979740.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGCAACA GCTCCGGAGA GACCCTGGGA GCAGACAGCG ACCTCAGCAG CAGTGCGGGC 
GATGGCCCAG GCGGCGAGGG CAGCGCCCAC TTGGCAGGCT CTCGAGGCAC CTTGTCTGAC 
AGTGAGATCG AGACCAACTC TGCCACCAGT GCCATCTTTG GTAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCTGAAGA TGTCAGCCAG 
CGAGTCTACC TCTACGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACG CCGATGGCCC ACGAGATCTG CTACTCTGTG 
CTGTGTCTCT TCTCGTATGT GGCTGCAGTC CGCAGCAGTG AGGAGGACCT CCGGACCCCG 
CCCCGGCCCC TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979741.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835542.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

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CloneID	OSm66085
Clone ID Related Accession (Same CDS sequence)	XM_027979741.1
Accession Version	XM_027979741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGCCTGCT GGCCACACTC CTGCACAATC TCATCTCGCA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGAGATC TTTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGCTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA CGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ACTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGT GAAGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCGATGGCC CACGAGATCT GCTACTCTGT GCTGTGTCTC 
TTCTCGTATG TGGCTGCAGT CCGCAGCAGT GAGGAGGACC TCCGGACCCC GCCCCGGCCC 
CTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835542.1
CDS	206..4957
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPLSS

Target ORF information:

RefSeq Version	XM_027979741.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979741.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAGTTCTGG 
GAAGACGCGT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GAATGGGCAT GGACCAGGGT 
CCCCAGGAAA TGATAGACAG GTACCTGTCC CTGGGAGAAC ACGACCGGAA GCGCCTGGAG 
GACGATGAAG ACCGCCTGCT GGCCACACTC CTGCACAATC TCATCTCGCA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGGAAG AAGGTGAGGC GCCTGATGGG AAAGTCCCAT 
ATTGGGCTTG TGTACAGTCA GCAAATCAAT GAAGTCCTTG ACCAGCTGGC CAACCTGAAT 
GGACGAGATC TTTCCATCCG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTCGTG 
GTACATGCAG GGACAGACAC GAATGGAGAT ATCTTTTTCA TGGAGGTGTG TGACGACTGC 
GTGGTGCTGC GCAGTAACAT CGGGACGCTG TACGAGCGCT GGTGGTACGA GAAACTCATC 
AACATGACCT ACTGCCCCAA GACCAAGGTG CTGTGCTTGT GGCGCAGAAA CGGCTCCGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAGAAG TGTCGGGAGC TGTACTACTG CGTGAAGGAC 
AGCATGGAGC GCGCCGCCGC CCGCCAGCAG AGCATCAAGC CGGGGCCGGA ACTGGGTGGC 
GAGTTCCCCG TGCAGGACAT GAAGACTGGT GAAGGGGGCT TGCTGCAGGT CACCCTCGAA 
GGGATCAACC TCAAGTTCAT GCACAACCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TCCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC GCCGATGGCC CACGAGATCT GCTACTCTGT GCTGTGTCTC 
TTCTCGTATG TGGCTGCAGT CCGCAGCAGT GAGGAGGACC TCCGGACCCC GCCCCGGCCC 
CTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979742.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835543.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X22, mRNA.

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CloneID	OSm66086
Clone ID Related Accession (Same CDS sequence)	XM_027979742.1
Accession Version	XM_027979742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835543.1
CDS	210..4952
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_027979742.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979742.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979743.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835544.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

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CloneID	OSm66087
Clone ID Related Accession (Same CDS sequence)	XM_027979743.1
Accession Version	XM_027979743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGCGATGGC 
CCAGGCGGCG AGGGCAGCGC CCACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGTGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCTG AAGATGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835544.1
CDS	210..4949
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_027979743.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979743.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGAGC 
AACAGCTCCG GAGAGACCCT GGGAGCAGAC AGCGACCTCA GCAGCAGTGC GGGCGATGGC 
CCAGGCGGCG AGGGCAGCGC CCACTTGGCA GGCTCTCGAG GCACCTTGTC TGACAGTGAG 
ATCGAGACCA ACTCTGCCAC CAGTGCCATC TTTGGTAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCTG AAGATGTCAG CCAGCGAGTC 
TACCTCTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAC 
GCTGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAG 
TTCTGGGAAG ACGCGTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGAAT GGGCATGGAC 
CAGGGTCCCC AGGAAATGAT AGACAGGTAC CTGTCCCTGG GAGAACACGA CCGGAAGCGC 
CTGGAGGACG ATGAAGACCG CCTGCTGGCC ACACTCCTGC ACAATCTCAT CTCGCACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT GATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGTCAGCAA ATCAATGAAG TCCTTGACCA GCTGGCCAAC 
CTGAATGGAC GAGATCTTTC CATCCGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTCGTGGTAC ATGCAGGGAC AGACACGAAT GGAGATATCT TTTTCATGGA GGTGTGTGAC 
GACTGCGTGG TGCTGCGCAG TAACATCGGG ACGCTGTACG AGCGCTGGTG GTACGAGAAA 
CTCATCAACA TGACCTACTG CCCCAAGACC AAGGTGCTGT GCTTGTGGCG CAGAAACGGC 
TCCGAGACCC AGCTCAACAA GTTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGCGTG 
AAGGACAGCA TGGAGCGCGC CGCCGCCCGC CAGCAGAGCA TCAAGCCGGG GCCGGAACTG 
GGTGGCGAGT TCCCCGTGCA GGACATGAAG ACTGGTGAAG GGGGCTTGCT GCAGGTCACC 
CTCGAAGGGA TCAACCTCAA GTTCATGCAC AACCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979744.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835545.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OSm66088
Clone ID Related Accession (Same CDS sequence)	XM_027979744.1
Accession Version	XM_027979744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGCGATGG CCCAGGCGGC 
GAGGGCAGCG CCCACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGTGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTAAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTCTTCT GAAGATGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GCCTGCTGGC CACACTCCTG CACAATCTCA TCTCGCACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGAGATCTTT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGCTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAACGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAACT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGTGAA GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835545.1
CDS	209..4939
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_027979744.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979744.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTGAG CAACAGCTCC 
GGAGAGACCC TGGGAGCAGA CAGCGACCTC AGCAGCAGTG CGGGCGATGG CCCAGGCGGC 
GAGGGCAGCG CCCACTTGGC AGGCTCTCGA GGCACCTTGT CTGACAGTGA GATCGAGACC 
AACTCTGCCA CCAGTGCCAT CTTTGGTAAA GCCCATAGCT TGAAGCCAAG TGTAAAGGAG 
AAGCTGGTGG GCAGCCCAGT TCGCTCTTCT GAAGATGTCA GCCAGCGAGT CTACCTCTAC 
GAGGGACTCC TAGGAAGGGA CAAAGGATCG ATGTGGGACC AGTTAGAGGA CGCTGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA GTTCTGGGAA 
GACGCGTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGAA TGGGCATGGA CCAGGGTCCC 
CAGGAAATGA TAGACAGGTA CCTGTCCCTG GGAGAACACG ACCGGAAGCG CCTGGAGGAC 
GATGAAGACC GCCTGCTGGC CACACTCCTG CACAATCTCA TCTCGCACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TGATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGTCAGCA AATCAATGAA GTCCTTGACC AGCTGGCCAA CCTGAATGGA 
CGAGATCTTT CCATCCGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTCGTGGTA 
CATGCAGGGA CAGACACGAA TGGAGATATC TTTTTCATGG AGGTGTGTGA CGACTGCGTG 
GTGCTGCGCA GTAACATCGG GACGCTGTAC GAGCGCTGGT GGTACGAGAA ACTCATCAAC 
ATGACCTACT GCCCCAAGAC CAAGGTGCTG TGCTTGTGGC GCAGAAACGG CTCCGAGACC 
CAGCTCAACA AGTTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGCGT GAAGGACAGC 
ATGGAGCGCG CCGCCGCCCG CCAGCAGAGC ATCAAGCCGG GGCCGGAACT GGGTGGCGAG 
TTCCCCGTGC AGGACATGAA GACTGGTGAA GGGGGCTTGC TGCAGGTCAC CCTCGAAGGG 
ATCAACCTCA AGTTCATGCA CAACCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979745.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835546.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OSm66089
Clone ID Related Accession (Same CDS sequence)	XM_027979745.1
Accession Version	XM_027979745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835546.1
CDS	209..4891
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIELWSKHQEVKKQKALEKQRPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_027979745.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979745.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGAA 
TTGTGGAGCA AGCACCAGGA AGTGAAAAAG CAAAAAGCTT TGGAAAAACA GAGGCCTGAA 
GTAATCAAAC CCACCTTTGA CCTTGGTGAG ACCGACGAGA AAAAGTCCCA GGTCAGCGCA 
GACAGTGGTG TGAGCCTGAC ATCCGGTTCC CAGAGGACCG ATCCAGACTC TGTCGTCGGC 
GTGAGTCCCG CCGTGATGAT CCGAAGCTCA AGTCAGGACT CTGAAGTTAG CACCGTGGTG 
AGCAACAGCT CCGGAGAGAC CCTGGGAGCA GACAGCGACC TCAGCAGCAG TGCGGGCGAT 
GGCCCAGGCG GCGAGGGCAG CGCCCACTTG GCAGGCTCTC GAGGCACCTT GTCTGACAGT 
GAGATCGAGA CCAACTCTGC CACCAGTGCC ATCTTTGGTA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CTGAAGATGT CAGCCAGCGA 
GTCTACCTCT ACGAGGGACT CCTAGGAAGG GACAAAGGAT CGATGTGGGA CCAGTTAGAG 
GACGCTGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAGTTCTGGG AAGACGCGTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG AATGGGCATG 
GACCAGGGTC CCCAGGAAAT GATAGACAGG TACCTGTCCC TGGGAGAACA CGACCGGAAG 
CGCCTGGAGG ACGATGAAGA CCGCCTGCTG GCCACACTCC TGCACAATCT CATCTCGCAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTGATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGTCAG CAAATCAATG AAGTCCTTGA CCAGCTGGCC 
AACCTGAATG GACGAGATCT TTCCATCCGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTCGTGG TACATGCAGG GACAGACACG AATGGAGATA TCTTTTTCAT GGAGGTGTGT 
GACGACTGCG TGGTGCTGCG CAGTAACATC GGGACGCTGT ACGAGCGCTG GTGGTACGAG 
AAACTCATCA ACATGACCTA CTGCCCCAAG ACCAAGGTGC TGTGCTTGTG GCGCAGAAAC 
GGCTCCGAGA CCCAGCTCAA CAAGTTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGC 
GTGAAGGACA GCATGGAGCG CGCCGCCGCC CGCCAGCAGA GCATCAAGCC GGGGCCGGAA 
CTGGGTGGCG AGTTCCCCGT GCAGGACATG AAGACTGGTG AAGGGGGCTT GCTGCAGGTC 
ACCCTCGAAG GGATCAACCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979746.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835547.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

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CloneID	OSm66090
Clone ID Related Accession (Same CDS sequence)	XM_027979746.1
Accession Version	XM_027979746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGCCT GCTGGCCACA CTCCTGCACA ATCTCATCTC GCACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGAG ATCTTTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG CTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAACGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAACTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGTGAAGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835547.1
CDS	209..4885
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_027979746.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979746.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG TATTTGGGCT AAATACTCTA ATGGAGATTG TTACTGAAGC CGGCCCTGGG 
AGTGGTGAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGCAAC 
AGCTCCGGAG AGACCCTGGG AGCAGACAGC GACCTCAGCA GCAGTGCGGG CGATGGCCCA 
GGCGGCGAGG GCAGCGCCCA CTTGGCAGGC TCTCGAGGCA CCTTGTCTGA CAGTGAGATC 
GAGACCAACT CTGCCACCAG TGCCATCTTT GGTAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCTGAAG ATGTCAGCCA GCGAGTCTAC 
CTCTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGACGCT 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAGTTC 
TGGGAAGACG CGTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGAATGGG CATGGACCAG 
GGTCCCCAGG AAATGATAGA CAGGTACCTG TCCCTGGGAG AACACGACCG GAAGCGCCTG 
GAGGACGATG AAGACCGCCT GCTGGCCACA CTCCTGCACA ATCTCATCTC GCACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTGAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG TCAGCAAATC AATGAAGTCC TTGACCAGCT GGCCAACCTG 
AATGGACGAG ATCTTTCCAT CCGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTC 
GTGGTACATG CAGGGACAGA CACGAATGGA GATATCTTTT TCATGGAGGT GTGTGACGAC 
TGCGTGGTGC TGCGCAGTAA CATCGGGACG CTGTACGAGC GCTGGTGGTA CGAGAAACTC 
ATCAACATGA CCTACTGCCC CAAGACCAAG GTGCTGTGCT TGTGGCGCAG AAACGGCTCC 
GAGACCCAGC TCAACAAGTT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGCGTGAAG 
GACAGCATGG AGCGCGCCGC CGCCCGCCAG CAGAGCATCA AGCCGGGGCC GGAACTGGGT 
GGCGAGTTCC CCGTGCAGGA CATGAAGACT GGTGAAGGGG GCTTGCTGCA GGTCACCCTC 
GAAGGGATCA ACCTCAAGTT CATGCACAAC CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_027979747.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027835548.1 Ovis aries MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

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CloneID	OSm66091
Clone ID Related Accession (Same CDS sequence)	XM_027979747.1
Accession Version	XM_027979747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	MAP kinase-activating death domain protein isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027835548.1
CDS	208..4773
Translation	MVQKKKSCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMTLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRVPKEKGEAGPGSRGKEGPRAPCVSEEVGPKTSESSPSLQPPSADSTPDVSQSPRARRRAKAGTRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGLPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMLYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPVLPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLRGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSGSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPSVDPLTHAALGDASEVAIDELQNQKEAEEPGLDGEHSQENPPLRSSSSTTASSSPSTVIHGASSEPVNSTEVDDKAAGGVPKSLPTMPPGVGKCSVDRRQPETGEGSVRRQASDSPCLQPQYGVPPEEDDEQGESYTPRLSQHASGHRGQKLLRPNSLKLASDSDAESDSRASSPTSTVSNNSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKSTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRASNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEEEARQDIIPDVEVSRKVYKGMLDLLKCTVLSLEQSFAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVSTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAPGKSPKELDTRSLKEENFVASIGPEVIKPTFDLGETDEKKSQVSADSGVSLTSGSQRTDPDSVVGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSSAGDGPGGEGSAHLAGSRGTLSDSEIETNSATSAIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISHMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTLYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_027979747.1
Organism	Ovis aries(sheep)
Definition	Ovis aries MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027979747.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTC CTGTCCTCGG TTACTTGACT ACCTGGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGACAG TGTGGCCCAG ACTCCGGAAC TGCTACGGCG GTACCCGCTA 
GAGGACCACG CGGAGTTTCC CCTGCCCCCG GATGTCGTGT TCTTCTGCCA GCCAGAGGGC 
TGCCTAAGCG TGCGGCAGCG GCGCATGACC CTGCGGGACG ACACCTCTTT TGTCTTCACC 
CTCACCGACA AGGACACTGG CGTCACCCGT TATGGCATCT GCGTCAACTT CTACCGCTCC 
TTCCAAAAGC GTGTGCCTAA GGAAAAAGGG GAGGCCGGGC CAGGGTCCCG GGGGAAGGAA 
GGGCCCCGAG CCCCCTGCGT CTCCGAGGAG GTGGGCCCTA AGACCTCAGA GAGCAGCCCG 
TCCCTACAGC CACCCAGTGC CGACTCCACC CCGGACGTGA GCCAGTCTCC GCGGGCCAGA 
CGCAGGGCCA AGGCAGGGAC CCGGTCCCGC AACAGCACGC TGACGTCCCT GTGCGTGCTC 
AGCCACTACC CCTTCTTCTC CACCTTCCGC GAATGTCTGT ACACCCTCAA GCGTCTGGTG 
GACTGCTGCA GCGAGCGCCT GCTGGGCAAG AAACTGGGCC TCCCTCGAGG CATACAGAGG 
GACACCATGT GGCGCATCTT CACCGGATCG TTGTTAGTGG AGGAGAAGTC GAGCGCCCTG 
CTGCATGACC TCCGGGAGAT TGAGGCCTGG ATCTACCGCT TGCTGCGCTC CCCAGTACCT 
GTCTCGGGGC AGAAACGAGT GGACATCGAG GTCCTGCCCC AGGAGCTCCA GCAGGCTCTG 
ACCTTTGCTC TCCCAGACCC CTCTCGATTC ACCCTAGTGG ACTTCCCGCT GCACCTTCCC 
CTGGAGCTTC TGGGTGTGGA CGCCTGTCTA CAGGTGCTCA CCTGCATCCT GTTAGAACAC 
AAGGTCGTGC TCCAGTCCCG AGACTACAAC GCACTCTCCA TGTCTGTGAT GGCGTTTGTG 
GCGATGCTGT ACCCCTTGGA GTATATGTTT CCTGTTATCC CACTGCTGCC CACCTGCATG 
GCGTCGGCAG AGCAGCTGCT ATTGGCTCCA ACACCATACA TCATCGGGGT CCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TGTGGCTGGT AGATCTGGAC 
AGCAACAGGG TGATTGCCCC CACCAACGCA GAAGTGCTGC CTGTCCTGCC AGAACCGGAA 
TCGTTAGAGT TGAAGAAGCA TTTGAAGCAG GCCCTCGCCA GCATGAGTCT CAACACCCAA 
CCCATCCTCA ATCTGGAGAA ATTCCACGAG GGCCAGGAGA TCCCCCTTCT CCTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCTTCC ACCGAGTTCA ACCCGCTCAT ATACGGCAAT 
GATGTGGATT CGGTAGATGT CGCAACCAGA GTGGCCATGG TCCGTTTCTT CAACTCCCCC 
AACGTGCTGC GGGGCTTTCA GATGCACACG CGTACCCTAC GTCTCTTCCC CCGGCCCGTG 
GTGGCTTTCC AAGCCGGCTC CTTTCTAGCC TCACGCCCCC GGCAGACTCC TTTCGCTGAA 
AAACTGGCCA GGACTCAGGC CGTGGAGTAC TTTGGAGAAT GGATCCTGAA CCCCACCAAC 
TACGCCTTCC AGCGGATTCA CAACAACATG TTCGATCCAG CTCTGATTGG CGACAAGCCC 
AAGTGGTACG CCCACCAGCT GCAGCCCATT CACTATCGAG TGTATGACAG CGGTTCCCAG 
CTGGCCGAGG CACTGAGCGT ACCGCCCGAG CGAGACTCTG ACTCTGACCC AACTGATGAC 
AGCGGGAGTG ACAGCATGGA TTATGACGAC TCGAGCTCTT CTTACTCTTC CCTTGGTGAC 
TTTGTCAGCG AGATGATGAA GTGCGACATC AACGGTGATA CTCCTAGCGT GGACCCTCTG 
ACACACGCGG CGCTGGGGGA TGCCAGCGAG GTGGCGATTG ATGAGCTACA GAACCAGAAG 
GAGGCAGAGG AACCCGGCCT GGATGGCGAG CACTCTCAGG AAAACCCGCC GCTGCGCTCT 
AGCTCCAGCA CCACTGCCAG CAGTAGCCCC AGCACCGTCA TCCACGGCGC CAGCTCTGAA 
CCTGTCAACT CAACAGAGGT GGACGATAAG GCGGCAGGAG GCGTCCCCAA GTCCCTCCCC 
ACCATGCCTC CTGGCGTGGG CAAGTGCAGC GTAGACAGAC GTCAGCCAGA AACGGGAGAA 
GGTTCAGTGC GCCGGCAAGC CTCTGACAGT CCGTGCCTCC AGCCCCAGTA TGGCGTTCCC 
CCTGAGGAAG ACGATGAGCA GGGTGAAAGT TACACCCCCC GACTCAGCCA GCATGCCAGT 
GGCCATCGGG GTCAAAAGCT GCTGCGGCCC AACAGCTTGA AACTGGCAAG TGACTCAGAC 
GCAGAGTCGG ACTCCCGAGC GAGTTCGCCC ACCTCCACCG TCTCCAACAA CAGCACGGAG 
GGCTTCGGGG GCATCATGTC TTTCGCCAGC AGCCTGTATC GGAACCACAG CACCAGCTTC 
AGCCTTTCAA ACCTCACGCT GCCCACCAAA GGAGCCCGAG AGAAGAGCAC GCCCTTCCCC 
AGTCTGAAAG GAAACAGGCG GGCCTTAGTG GACCAGAAGT CATCGGTTAT TAAACATAGC 
CCGACCGTGA AAAGAGAGCC TCCGTCACCC CAGGGCCGGG CCAGCAACTC TAGTGAGAAC 
CAGCAGTTCC TGAAGGAGGT GGTGCACAGC GTGCTGGACG GCCAGGGTGT CGGCTGGCTC 
AACATGAAGA AGGTGCGGCG GCTGCTGGAG AGCGAGCAGC TGCGCGTCTT TGTCCTGAGC 
AAGCTGAACC GCACAGTGCA GTCCGAGGAG GAGGCCCGGC AGGACATCAT CCCCGACGTG 
GAGGTCAGCC GGAAGGTGTA CAAGGGCATG TTGGACCTGC TCAAGTGCAC GGTCCTCAGT 
CTGGAGCAGT CCTTTGCCCA TGCCGGCCTG GGTGGCATGG CCAGCATCTT CGGGCTCCTG 
GAGATCGCCC AGACCCACTA CTACAGTAAA GAACCAGACA AGCGGAAGAG AAGTCCCACG 
GAGAGCGTCA GTACACCAGT CGGCAAGGAT CCTGGCCTGG CTGGGCGGGG GGACCCAAAG 
GCCATGGCCC AGCTGAGGGT CCCCCAGCTG GGACCTCGGG CACCAAGTGC CCCAGGAAAG 
AGTCCCAAGG AGCTGGACAC CAGGAGTCTA AAGGAAGAGA ACTTTGTGGC ATCTATTGGG 
CCTGAAGTAA TCAAACCCAC CTTTGACCTT GGTGAGACCG ACGAGAAAAA GTCCCAGGTC 
AGCGCAGACA GTGGTGTGAG CCTGACATCC GGTTCCCAGA GGACCGATCC AGACTCTGTC 
GTCGGCGTGA GTCCCGCCGT GATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGCA ACAGCTCCGG AGAGACCCTG GGAGCAGACA GCGACCTCAG CAGCAGTGCG 
GGCGATGGCC CAGGCGGCGA GGGCAGCGCC CACTTGGCAG GCTCTCGAGG CACCTTGTCT 
GACAGTGAGA TCGAGACCAA CTCTGCCACC AGTGCCATCT TTGGTAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCTGA AGATGTCAGC 
CAGCGAGTCT ACCTCTACGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAGTTCT GGGAAGACGC GTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGAATGGGC 
ATGGACCAGG GTCCCCAGGA AATGATAGAC AGGTACCTGT CCCTGGGAGA ACACGACCGG 
AAGCGCCTGG AGGACGATGA AGACCGCCTG CTGGCCACAC TCCTGCACAA TCTCATCTCG 
CACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTGATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGT CAGCAAATCA ATGAAGTCCT TGACCAGCTG 
GCCAACCTGA ATGGACGAGA TCTTTCCATC CGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTCG TGGTACATGC AGGGACAGAC ACGAATGGAG ATATCTTTTT CATGGAGGTG 
TGTGACGACT GCGTGGTGCT GCGCAGTAAC ATCGGGACGC TGTACGAGCG CTGGTGGTAC 
GAGAAACTCA TCAACATGAC CTACTGCCCC AAGACCAAGG TGCTGTGCTT GTGGCGCAGA 
AACGGCTCCG AGACCCAGCT CAACAAGTTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGCGTGAAGG ACAGCATGGA GCGCGCCGCC GCCCGCCAGC AGAGCATCAA GCCGGGGCCG 
GAACTGGGTG GCGAGTTCCC CGTGCAGGAC ATGAAGACTG GTGAAGGGGG CTTGCTGCAG 
GTCACCCTCG AAGGGATCAA CCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

MADD cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

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