TTC3 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_012130614.1	XP_011986004.1	E3 ubiquitin-protein ligase TTC3 isoform X4
XM_012130619.2	XP_011986009.1	E3 ubiquitin-protein ligase TTC3 isoform X7
XM_027961634.1	XP_027817435.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_027961647.1	XP_027817448.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_012130613.1	XP_011986003.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_012130617.2	XP_011986007.1	E3 ubiquitin-protein ligase TTC3 isoform X6
XM_012105081.2	XP_011960471.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_012130610.1	XP_011986000.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_012130611.1	XP_011986001.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_012130615.2	XP_011986005.1	E3 ubiquitin-protein ligase TTC3 isoform X5
XM_012130618.2	XP_011986008.1	E3 ubiquitin-protein ligase TTC3 isoform X7
XM_012130622.2	XP_011986012.1	E3 ubiquitin-protein ligase TTC3 isoform X7
XM_027961627.1	XP_027817428.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_012130612.1	XP_011986002.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_012130616.2	XP_011986006.1	E3 ubiquitin-protein ligase TTC3 isoform X5
XM_012130621.2	XP_011986011.1	E3 ubiquitin-protein ligase TTC3 isoform X7
XM_027961621.1	XP_027817422.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_027961639.1	XP_027817440.1	E3 ubiquitin-protein ligase TTC3 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv13960	XM_012130610.1 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13961	XM_012130613.1 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13961	XM_012130612.1 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13961	XM_012130611.1 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13962	XM_012130614.1 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13963	XM_012130615.2 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13963	XM_012130616.2 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13964	XM_012130617.2 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13965	XM_012130618.2 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13965	XM_012130622.2 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13965	XM_012130619.2 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv13965	XM_012130621.2 Latest version!	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSm59213	XM_027961634.1 Latest version!	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm59213	XM_027961621.1 Latest version!	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm59213	XM_027961627.1 Latest version!	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm59214	XM_027961639.1 Latest version!	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm59215	XM_027961647.1 Latest version!	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm37457	XM_012105081.2 Latest version!	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

TTC3 ( XM_012130610.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986000.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv13960
Clone ID Related Accession (Same CDS sequence)	XM_012130610.1
Accession Version	XM_012130610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACCAA CGGTTTGCTA CAAAGGGTGG TGTACCATGG CCGATTTTGC CCAGGGAAGT 
TTTTCGGTAG CAGATTATGC CTTGTTAGAA GATTGCCCTT ACATGGACGA TTGTGTCTTT 
GCCCCTGAGT TTATGTCCAG TGATTATGTT CGTGTAACTC AGCTTTACTG TGATGGAGTG 
GGTAGACAGT ATAAAGATTA TACCCAAACT GAGGGAAACT TAGAATTTGA CATCTGCACT 
ATGTGGTGTA GTAAACCTGT TTCTGTCCTG CAAGATTACT GTGATGCCAT TAAAGTCCAC 
ATCTTCTGGC CACTTCTGTT TCAACGTCAG CAGAGCTCCA TAATAACACG GATGCACTCC 
TGTGTAAGGA CCAGCGGTTC TCATGCTTGT GAGATAAGTT TGAAGAAATT ACAGCAGCTT 
GAGCTGATGG AAGATATTGT GGATTTGGCA AAGAAAGTTG TGAATGATAC ATTCTTTATC 
GGAGGCTTAT TGAGAATTGG CTATAAAATA GAGAATAAAA TCTTGGCAAT GGAAGAAGCT 
TTGAATTGGG TGAAATCCAC AGGCGATGTA ACAATTTTAT CTAAATTAGG AGCAGTTGAC 
AATTGTTGGC GTATGTTAAG TATTTTCTTT ACTGAATACA AGTATCACAT AACTAAAATT 
GTAACAGAAA ACTGCAGTTT GCTTGAAGAA TTTAAAACTC AAAGTTGTGC GGAGTGTCTA 
GAGCAAGGAG AACTAATGAA AATGAAAGGA AATGAAGAGT TTTCCAAAGA AAGATATGAT 
ATAGCTATTA TCTATTACTC CAGAGCCATT GAATGTAGGC CTGAAAATCA CCTGCTTTAC 
GGTAACCGAG CTCTTTGTTT TCTTCGTACT GGACAATTCA GAAATGCACT TGGTGATGGA 
AAGAGAGCTA CCATTCTGAA GAACAATTGG ACAAAGGGTC ATTATCGTTA TTGTGCTGCT 
CTTTCTATGC TGGGGGAATA TGACTGGGCC CTGCAAGCAA ACATAAAAGC TCAAAAACTC 
TGTAAAAATG ACCCTGAGGG AATCAAGGAT CTAATTCAGC AGCATGTAAA GTTACAAAAA 
CAGATAGAAG ACCTGCAAGG TCGAACACCA GCTAGTAATC CAGTCAAAGC CTTCTGTGAA 
AGCAGGGCCT ACATACCTAG TTTATCAGCA CCTGCATTTA GTACTTCACT TAACTTTGTG 
GAAACTGAAA AAAATTTCAG AAAAACTAAC CACGAAATGG CAAACGGTGG TAATCAGAAT 
CCAAAGGTGG TAGATGAAGC TCTGAAAGTA GATGATTGTG ACTGTCATCC TGAATTTGTA 
CCACCATCAA GTCAGCCTCC AAAATATAAA GGGAAACAAA AAGCTCGAAA CAATGAATTG 
GAGAAGTTCA GCCCTGGTTC ACAAGAAACT TTACCAGTAG ATTTGAAAAA CATCTTGGAG 
AAACAGTTTT CTAAATCTTC CAGAGCTGCA CACCAGGATT TTGCTAATAT AATGAAAATG 
CTGAGAAGCT TAATCCAGGA TGGCTACACG GCCTTGCTGG AGCAGCGCTG CCGCAGTGCT 
GCCCAGGCCT TCACAGAGTT GCTCAACGGT TTAGATCCTC AAAAAATAAA GCAATTGAAT 
CTGGCCATGA TTAACTACGT GTTAGTAGTC TACGGACTTG CCATTTCACT CCTTGGAATA 
GGACAGCCTG AGGAGTTATC TGAAGCTGAA AACCAATTTA AGAGGATGAT TGAACACTAC 
CCCAATGAGG GACTTGATTG CTTGGCCTAC TGTGGAATTG GAAAAGTATA TTTGAAAAAA 
AACAGATTTT TAGAAGCTCT TAATCATTTT GAGAAAGCAA GAACCTTGAT CTGTCGTCTT 
CCTGGAGTGT TAACTTGGCC CACGAGTAAT GTGATTATTG AGGAGACTCA GCCAGGGAAG 
ATAAAGATGC TGTTAGAAAA ATTCGTTGAA GAATGCAGGT TCCCTCCAGT GCCGGATGCC 
ACTTGTTGCT ATCAGAAGTG CCATGGATAT TCCAAAATTC AGATATATAT AACCGATCCA 
GACTTCAAGG GTTTTATACG AATCAGCTGT TGCCAGTATT GTAAAATAGA ATTTCACATG 
AACTGCTGGA AGAAGTTAAA AACAACAACC TTCAATGATA AAATTGACAA GGATTTTCTG 
CAAGGAATTT GTCTTACTCC TGATTGTAAA GGAATCATTT CAAAGATTAT CATCTTCAGC 
AGTGGTGGTC AAGTTAAATG TGAATTTGAA CACAAGGTCA TTAAAGAAAA GCTTCCTCCA 
AGACCTATTC TGAAACAGAA ATGTTCTAGC CTAGAGAAGT TGAGGCTGAA AGAAGACAAA 
AAATTGAAAC GAAAGCTCCA AAAAAAGGAA GCAAAAAAAT TAGCACAAGA AAGACTGGAA 
GAGGACCTAA GGGAAAGTAA CCCACCAAAA ACTGAAGAGC AGAGAGAAAC TATCAGCCGT 
GTCCAAAGTT GCCAGTTCCT TGATGACAGA ATTCTGCAGT GCATAAAGCA GTACGCTGAC 
AAGATTAAGT CCGGGATACT GAATACTTCC AAGCTTCTCA AAGAATTGCT TTCTTGGAAG 
GTTTTAACCA CAGAAGACTA CACAACGTGT TTTTCTAGCA GAAATTTCCT GAATGAAGCA 
GTGGACTATG TTATTCGTCA TTTGATCCAG GAAAAGAACA GAGTAAAGAC AAGGGTATTT 
CTGCATGTTT TGAGTGAGCT TAAAGAAGTG GAGCCAAAAC TAGCCACCTG GATCCAGAAA 
CTTAATAGCT TTGGCTTAGA TGCCACAGGA CCTTTCTTTT CTCGTTATGG GGCTCCTCTT 
AAGGAGCTTG ATTTTAGCAT CATGACTTTT CTCTGGAATG AGAAATATGG TCATAAGCTA 
GGCTCTATAG AAGGAAAGCA ACTTGATTAT TTCTATGAGC CTACGTCACT GAAGGAAGCG 
CGGTGTTTAA TATGGCTGCT GGAAGAACAC AGAGACAAGT TCCCGGCACT GCATAATGCC 
CTGGACGAGT TCTTTGATAT AATGGACAGC CGCTGCACTG TGTTAAGGAA ACAGGACAGT 
GGTGACGTGC CATGTAATTT TACCAAGATA AAAAACAAAG GCAAGAAAAA GAAGCCAAAA 
GATTCAAAGC CTATGTTAGT TGGATCTGGA ACACCTTCAG TCACACCAAA CAATGAGACT 
TTCACTTCAG GTGAAGACCA TATCAAACGC AGTTTAAATT CTGCGGGCCC CTTTGTAGTG 
CCTGATCACC TTCGGCAGGA TGTAGAAGAA TTTGAAGCTC TGTATGAACA GCATAGTAAT 
GAGTATGTTG TCCGTAATAA GAAGCTGTGG GACATAAACC CAAAACAAAA ATGTTCAACT 
CTATATGATT ATTTCTCTCA GTTGTTGGAG GAACATGGTC CCTTGGACAT GAGTAACAAG 
ATGTTTTCTG AAGAATATGA GTTCTTCCCA GAAGAAACTC GACAGATACT AGAAAAAGCA 
GGAGGTTTGA AAGCGTTTCT CCTCGGATGT CCTCGTTTTG TTGTGATTGA CAACTGTATT 
GCATTGAAGA AAGTTGCATT ACGGCTCAAG AAAAAGAGAA AGAAGAAAAA CATTAAGACA 
AAAGTAGAAG AAATTTCAAA AACAGGAGAG TATTTACGAG TTAAACTACC ACTGAATCCA 
ACTGCTAGGG AATTTAAACC AGATGTAAAG TCCAAACCAG TGTCTGACTT ACCTTTAGCA 
CCAGCTTCTG AGGACGTGAA GCCTCAGCAT GCATCTGCTA ATTCTCCCCA ATCAGCTCGT 
GACACTGGGA AGCCAAAACC AGTTTCCGAT AATTCTTCCA GATCAGTTTC TGAGGATGAG 
AAGCCCAAAG GGATATCTTC TGATTCTCCC AGGCCAGTCC TTGAGGAGAC CATTCACAAG 
CCAGTCTCCT CTAATTCTCC CAAACCAGTT CCCGAGGAAG TGAAAGCGAC CTATTGGCCT 
CGACCCCAGT TGGTCACAGG ATACTGTACA TATCTTCCCT TTCGAGGATT TGACATTACC 
CAGACACCAC CAGCCTTCAT CAGTGTGTTA CCGACTTTGC CCCAGTATAG CATTTACACC 
CCCCTGGCCA GCGTTCCTAC TGAGTATCAC CTGCAAAGAC CAATACCAGT GGTGCCGTCT 
TTTGTAGCCA GTGACATAAC GGAAGTGCAT TTTGAGGCTC ATCATTTCAG TGCTGAGAAT 
GCCCATGGTA ACCAGATTGC CTCCGAAACA CAGATCCTTG AGGAATCTTT GGAAACACCA 
GTGAAGTCAG AGTGCAGCTC TGATGGTGCT GATATAGCCC AGAGTGAGGC TAGCGGAAAC 
GAGGACTACT GTGGAGATTC TGCCAAGTGG GAAGTAAATC CAGAAAGCAC CAATAAAGTA 
ACAAATATTC CATGTACACA AGTGGTTGCT GTACAGGTGT CTTGGAATAT AACACATCAG 
GAAGTCAATA CTGAGCCATA TGGTCCTTTT GAGGCACAAC AGGGAGGTAT TTCACAGATT 
GAAAAGGAGT ACCAGGTTTT ACAAGAGCAG CTTAAGGAAG CATGTGAAAA TTATGAACAC 
AGCAATGTCA GGGGCTCAGA AGAAACCAAG GACTTGGAAG AAAAGTTGAA AAGGAACGTT 
GAAGAAAATA AGATCTCAAA GACAGAACTA GATTGGTTCC TTGAAGATTT GAACAAGGAA 
ATTAAAAAAT GGGAACAGGA GAAAAAAGAA ATCCAAGAAA GACTAAAGGC ACTGAAGAAG 
AAAATGAAAA AGGTGTTGAA TGCCAGCAGA ATGTATACAC AGGAAAACGA TGGAAAGGAA 
AAGGCACTTG AATTACTTCT GGATCAGTCC CTTGAAATCA GTAATACATT TACAAATGAG 
AAAATGAAAA TAGAAGAGAG TATAAAGAAA GGGAAAGAGC ATTATGAAGA GAGTCTTCAG 
AGGGCTCTGG ATGCAGAGGT ATCTGTGCTT GAAAACTGGA AGGATGTGGA GGTGTGTAAG 
TTACAGAGCA TGGAGTCACA GGCCGAAGGG CTTCTCAAGA ACTTGAAGCT AATGAGCAGT 
GATTCGGCCG CATATTCTGA CATGGACTCA GACATACATT CATGGGAATC GTTTCTTTCT 
GATGTTAGAG AAGAAATTGA GAAAGCAAAG TCTCAGTTTG AAGAACAAAT TCAGGCAATT 
AAAAATGGTT CTCGGCTCAG TGAACTTCCT AAAGTGCAGA TTTCTGAGCT TTCGTTTCCT 
ACCTGTAACA CAATTCATCC TAAATTACTT CCTGAGTCTT CAGGCCAGGA GGGTCAAGGT 
CCCTCCACTT CTGCAAGTCA TGGGACCGGA GACCAGACAG CAGCTCCCGA GGAGTCAAGT 
CTGGGTTCTG CTGCTGACAG CCCAGTGGAG TCGCCCTCCT GCAGCCAGAA AGCCCCCCAG 
CTGCCAGAGC GTGAGGGCAC TGGCCAGGCA GCTGGGCCAG AACTAGGCCT GATGGCTGAT 
CAGAAGTTAC CTGTTGCTCC AGAGCGTGCC ACTCGTCCAA GCCAGTCTCC AAAGAAGCCG 
TTCAATAGCA TTATTGAGCA CCTGTCGGTG GTGTTCCCTT GTTACAGCAG CACGGAGCTT 
GCTGGTTTTA TTAAAAAAGT TAGAAACAAA AACAAGACCT CTCTGTCTGG ATTGAGTATT 
GACGAAATTG TGCAAAGAGT GACACAACAC ATCCTTGATG AAGAGAGAAA GAAAAAGCCA 
AATCCAGGAA AGGACCGGAG GCCGTCTGCA CCCAGCTCTG CTGTTCCTGC ACCCAGGGCC 
GCCCAGGACC CGCCCTCCGT GCCTGTCAGA GCATCACCCG AAACCAGGGG GCCGAAGACC 
GAAGACGTCC CTGTGTCCTG TGCCAATTCC TGTGAAATGT GCCAAGAAGC ATTCAAGTCC 
AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AAGGGTGCTT CAAGCAGTGG 
TTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986000.1
CDS	4120..10119
Translation	MVPTVCYKGWCTMADFAQGSFSVADYALLEDCPYMDDCVFAPEFMSSDYVRVTQLYCDGVGRQYKDYTQTEGNLEFDICTMWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130610.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130610.1

ORF Insert Sequence:

ATGGTACCAA CGGTTTGCTA CAAAGGGTGG TGTACCATGG CCGATTTTGC CCAGGGAAGT 
TTTTCGGTAG CAGATTATGC CTTGTTAGAA GATTGCCCTT ACATGGACGA TTGTGTCTTT 
GCCCCTGAGT TTATGTCCAG TGATTATGTT CGTGTAACTC AGCTTTACTG TGATGGAGTG 
GGTAGACAGT ATAAAGATTA TACCCAAACT GAGGGAAACT TAGAATTTGA CATCTGCACT 
ATGTGGTGTA GTAAACCTGT TTCTGTCCTG CAAGATTACT GTGATGCCAT TAAAGTCCAC 
ATCTTCTGGC CACTTCTGTT TCAACGTCAG CAGAGCTCCA TAATAACACG GATGCACTCC 
TGTGTAAGGA CCAGCGGTTC TCATGCTTGT GAGATAAGTT TGAAGAAATT ACAGCAGCTT 
GAGCTGATGG AAGATATTGT GGATTTGGCA AAGAAAGTTG TGAATGATAC ATTCTTTATC 
GGAGGCTTAT TGAGAATTGG CTATAAAATA GAGAATAAAA TCTTGGCAAT GGAAGAAGCT 
TTGAATTGGG TGAAATCCAC AGGCGATGTA ACAATTTTAT CTAAATTAGG AGCAGTTGAC 
AATTGTTGGC GTATGTTAAG TATTTTCTTT ACTGAATACA AGTATCACAT AACTAAAATT 
GTAACAGAAA ACTGCAGTTT GCTTGAAGAA TTTAAAACTC AAAGTTGTGC GGAGTGTCTA 
GAGCAAGGAG AACTAATGAA AATGAAAGGA AATGAAGAGT TTTCCAAAGA AAGATATGAT 
ATAGCTATTA TCTATTACTC CAGAGCCATT GAATGTAGGC CTGAAAATCA CCTGCTTTAC 
GGTAACCGAG CTCTTTGTTT TCTTCGTACT GGACAATTCA GAAATGCACT TGGTGATGGA 
AAGAGAGCTA CCATTCTGAA GAACAATTGG ACAAAGGGTC ATTATCGTTA TTGTGCTGCT 
CTTTCTATGC TGGGGGAATA TGACTGGGCC CTGCAAGCAA ACATAAAAGC TCAAAAACTC 
TGTAAAAATG ACCCTGAGGG AATCAAGGAT CTAATTCAGC AGCATGTAAA GTTACAAAAA 
CAGATAGAAG ACCTGCAAGG TCGAACACCA GCTAGTAATC CAGTCAAAGC CTTCTGTGAA 
AGCAGGGCCT ACATACCTAG TTTATCAGCA CCTGCATTTA GTACTTCACT TAACTTTGTG 
GAAACTGAAA AAAATTTCAG AAAAACTAAC CACGAAATGG CAAACGGTGG TAATCAGAAT 
CCAAAGGTGG TAGATGAAGC TCTGAAAGTA GATGATTGTG ACTGTCATCC TGAATTTGTA 
CCACCATCAA GTCAGCCTCC AAAATATAAA GGGAAACAAA AAGCTCGAAA CAATGAATTG 
GAGAAGTTCA GCCCTGGTTC ACAAGAAACT TTACCAGTAG ATTTGAAAAA CATCTTGGAG 
AAACAGTTTT CTAAATCTTC CAGAGCTGCA CACCAGGATT TTGCTAATAT AATGAAAATG 
CTGAGAAGCT TAATCCAGGA TGGCTACACG GCCTTGCTGG AGCAGCGCTG CCGCAGTGCT 
GCCCAGGCCT TCACAGAGTT GCTCAACGGT TTAGATCCTC AAAAAATAAA GCAATTGAAT 
CTGGCCATGA TTAACTACGT GTTAGTAGTC TACGGACTTG CCATTTCACT CCTTGGAATA 
GGACAGCCTG AGGAGTTATC TGAAGCTGAA AACCAATTTA AGAGGATGAT TGAACACTAC 
CCCAATGAGG GACTTGATTG CTTGGCCTAC TGTGGAATTG GAAAAGTATA TTTGAAAAAA 
AACAGATTTT TAGAAGCTCT TAATCATTTT GAGAAAGCAA GAACCTTGAT CTGTCGTCTT 
CCTGGAGTGT TAACTTGGCC CACGAGTAAT GTGATTATTG AGGAGACTCA GCCAGGGAAG 
ATAAAGATGC TGTTAGAAAA ATTCGTTGAA GAATGCAGGT TCCCTCCAGT GCCGGATGCC 
ACTTGTTGCT ATCAGAAGTG CCATGGATAT TCCAAAATTC AGATATATAT AACCGATCCA 
GACTTCAAGG GTTTTATACG AATCAGCTGT TGCCAGTATT GTAAAATAGA ATTTCACATG 
AACTGCTGGA AGAAGTTAAA AACAACAACC TTCAATGATA AAATTGACAA GGATTTTCTG 
CAAGGAATTT GTCTTACTCC TGATTGTAAA GGAATCATTT CAAAGATTAT CATCTTCAGC 
AGTGGTGGTC AAGTTAAATG TGAATTTGAA CACAAGGTCA TTAAAGAAAA GCTTCCTCCA 
AGACCTATTC TGAAACAGAA ATGTTCTAGC CTAGAGAAGT TGAGGCTGAA AGAAGACAAA 
AAATTGAAAC GAAAGCTCCA AAAAAAGGAA GCAAAAAAAT TAGCACAAGA AAGACTGGAA 
GAGGACCTAA GGGAAAGTAA CCCACCAAAA ACTGAAGAGC AGAGAGAAAC TATCAGCCGT 
GTCCAAAGTT GCCAGTTCCT TGATGACAGA ATTCTGCAGT GCATAAAGCA GTACGCTGAC 
AAGATTAAGT CCGGGATACT GAATACTTCC AAGCTTCTCA AAGAATTGCT TTCTTGGAAG 
GTTTTAACCA CAGAAGACTA CACAACGTGT TTTTCTAGCA GAAATTTCCT GAATGAAGCA 
GTGGACTATG TTATTCGTCA TTTGATCCAG GAAAAGAACA GAGTAAAGAC AAGGGTATTT 
CTGCATGTTT TGAGTGAGCT TAAAGAAGTG GAGCCAAAAC TAGCCACCTG GATCCAGAAA 
CTTAATAGCT TTGGCTTAGA TGCCACAGGA CCTTTCTTTT CTCGTTATGG GGCTCCTCTT 
AAGGAGCTTG ATTTTAGCAT CATGACTTTT CTCTGGAATG AGAAATATGG TCATAAGCTA 
GGCTCTATAG AAGGAAAGCA ACTTGATTAT TTCTATGAGC CTACGTCACT GAAGGAAGCG 
CGGTGTTTAA TATGGCTGCT GGAAGAACAC AGAGACAAGT TCCCGGCACT GCATAATGCC 
CTGGACGAGT TCTTTGATAT AATGGACAGC CGCTGCACTG TGTTAAGGAA ACAGGACAGT 
GGTGACGTGC CATGTAATTT TACCAAGATA AAAAACAAAG GCAAGAAAAA GAAGCCAAAA 
GATTCAAAGC CTATGTTAGT TGGATCTGGA ACACCTTCAG TCACACCAAA CAATGAGACT 
TTCACTTCAG GTGAAGACCA TATCAAACGC AGTTTAAATT CTGCGGGCCC CTTTGTAGTG 
CCTGATCACC TTCGGCAGGA TGTAGAAGAA TTTGAAGCTC TGTATGAACA GCATAGTAAT 
GAGTATGTTG TCCGTAATAA GAAGCTGTGG GACATAAACC CAAAACAAAA ATGTTCAACT 
CTATATGATT ATTTCTCTCA GTTGTTGGAG GAACATGGTC CCTTGGACAT GAGTAACAAG 
ATGTTTTCTG AAGAATATGA GTTCTTCCCA GAAGAAACTC GACAGATACT AGAAAAAGCA 
GGAGGTTTGA AAGCGTTTCT CCTCGGATGT CCTCGTTTTG TTGTGATTGA CAACTGTATT 
GCATTGAAGA AAGTTGCATT ACGGCTCAAG AAAAAGAGAA AGAAGAAAAA CATTAAGACA 
AAAGTAGAAG AAATTTCAAA AACAGGAGAG TATTTACGAG TTAAACTACC ACTGAATCCA 
ACTGCTAGGG AATTTAAACC AGATGTAAAG TCCAAACCAG TGTCTGACTT ACCTTTAGCA 
CCAGCTTCTG AGGACGTGAA GCCTCAGCAT GCATCTGCTA ATTCTCCCCA ATCAGCTCGT 
GACACTGGGA AGCCAAAACC AGTTTCCGAT AATTCTTCCA GATCAGTTTC TGAGGATGAG 
AAGCCCAAAG GGATATCTTC TGATTCTCCC AGGCCAGTCC TTGAGGAGAC CATTCACAAG 
CCAGTCTCCT CTAATTCTCC CAAACCAGTT CCCGAGGAAG TGAAAGCGAC CTATTGGCCT 
CGACCCCAGT TGGTCACAGG ATACTGTACA TATCTTCCCT TTCGAGGATT TGACATTACC 
CAGACACCAC CAGCCTTCAT CAGTGTGTTA CCGACTTTGC CCCAGTATAG CATTTACACC 
CCCCTGGCCA GCGTTCCTAC TGAGTATCAC CTGCAAAGAC CAATACCAGT GGTGCCGTCT 
TTTGTAGCCA GTGACATAAC GGAAGTGCAT TTTGAGGCTC ATCATTTCAG TGCTGAGAAT 
GCCCATGGTA ACCAGATTGC CTCCGAAACA CAGATCCTTG AGGAATCTTT GGAAACACCA 
GTGAAGTCAG AGTGCAGCTC TGATGGTGCT GATATAGCCC AGAGTGAGGC TAGCGGAAAC 
GAGGACTACT GTGGAGATTC TGCCAAGTGG GAAGTAAATC CAGAAAGCAC CAATAAAGTA 
ACAAATATTC CATGTACACA AGTGGTTGCT GTACAGGTGT CTTGGAATAT AACACATCAG 
GAAGTCAATA CTGAGCCATA TGGTCCTTTT GAGGCACAAC AGGGAGGTAT TTCACAGATT 
GAAAAGGAGT ACCAGGTTTT ACAAGAGCAG CTTAAGGAAG CATGTGAAAA TTATGAACAC 
AGCAATGTCA GGGGCTCAGA AGAAACCAAG GACTTGGAAG AAAAGTTGAA AAGGAACGTT 
GAAGAAAATA AGATCTCAAA GACAGAACTA GATTGGTTCC TTGAAGATTT GAACAAGGAA 
ATTAAAAAAT GGGAACAGGA GAAAAAAGAA ATCCAAGAAA GACTAAAGGC ACTGAAGAAG 
AAAATGAAAA AGGTGTTGAA TGCCAGCAGA ATGTATACAC AGGAAAACGA TGGAAAGGAA 
AAGGCACTTG AATTACTTCT GGATCAGTCC CTTGAAATCA GTAATACATT TACAAATGAG 
AAAATGAAAA TAGAAGAGAG TATAAAGAAA GGGAAAGAGC ATTATGAAGA GAGTCTTCAG 
AGGGCTCTGG ATGCAGAGGT ATCTGTGCTT GAAAACTGGA AGGATGTGGA GGTGTGTAAG 
TTACAGAGCA TGGAGTCACA GGCCGAAGGG CTTCTCAAGA ACTTGAAGCT AATGAGCAGT 
GATTCGGCCG CATATTCTGA CATGGACTCA GACATACATT CATGGGAATC GTTTCTTTCT 
GATGTTAGAG AAGAAATTGA GAAAGCAAAG TCTCAGTTTG AAGAACAAAT TCAGGCAATT 
AAAAATGGTT CTCGGCTCAG TGAACTTCCT AAAGTGCAGA TTTCTGAGCT TTCGTTTCCT 
ACCTGTAACA CAATTCATCC TAAATTACTT CCTGAGTCTT CAGGCCAGGA GGGTCAAGGT 
CCCTCCACTT CTGCAAGTCA TGGGACCGGA GACCAGACAG CAGCTCCCGA GGAGTCAAGT 
CTGGGTTCTG CTGCTGACAG CCCAGTGGAG TCGCCCTCCT GCAGCCAGAA AGCCCCCCAG 
CTGCCAGAGC GTGAGGGCAC TGGCCAGGCA GCTGGGCCAG AACTAGGCCT GATGGCTGAT 
CAGAAGTTAC CTGTTGCTCC AGAGCGTGCC ACTCGTCCAA GCCAGTCTCC AAAGAAGCCG 
TTCAATAGCA TTATTGAGCA CCTGTCGGTG GTGTTCCCTT GTTACAGCAG CACGGAGCTT 
GCTGGTTTTA TTAAAAAAGT TAGAAACAAA AACAAGACCT CTCTGTCTGG ATTGAGTATT 
GACGAAATTG TGCAAAGAGT GACACAACAC ATCCTTGATG AAGAGAGAAA GAAAAAGCCA 
AATCCAGGAA AGGACCGGAG GCCGTCTGCA CCCAGCTCTG CTGTTCCTGC ACCCAGGGCC 
GCCCAGGACC CGCCCTCCGT GCCTGTCAGA GCATCACCCG AAACCAGGGG GCCGAAGACC 
GAAGACGTCC CTGTGTCCTG TGCCAATTCC TGTGAAATGT GCCAAGAAGC ATTCAAGTCC 
AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AAGGGTGCTT CAAGCAGTGG 
TTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130613.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986003.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

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CloneID	OOv13961
Clone ID Related Accession (Same CDS sequence)	XM_012130613.1 , XM_012130611.1 , XM_012130612.1
Accession Version	XM_012130613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TTGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACG TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CAGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986003.1
CDS	2668..8631
Translation	MADFAQGSFSVADYALLEDCPYMDDCVFAPEFMSSDYVRVTQLYCDGVGRQYKDYTQTEGNLEFDICTMWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130613.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130613.1

ORF Insert Sequence:

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TTGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACG TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CAGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130611.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986001.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

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CloneID	OOv13961
Clone ID Related Accession (Same CDS sequence)	XM_012130613.1 , XM_012130611.1 , XM_012130612.1
Accession Version	XM_012130611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TTGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACG TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CAGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986001.1
CDS	140..6103
Translation	MADFAQGSFSVADYALLEDCPYMDDCVFAPEFMSSDYVRVTQLYCDGVGRQYKDYTQTEGNLEFDICTMWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130611.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130611.1

ORF Insert Sequence:

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TTGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACG TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CAGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130612.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986002.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

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CloneID	OOv13961
Clone ID Related Accession (Same CDS sequence)	XM_012130613.1 , XM_012130611.1 , XM_012130612.1
Accession Version	XM_012130612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TTGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACG TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CAGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986002.1
CDS	137..6100
Translation	MADFAQGSFSVADYALLEDCPYMDDCVFAPEFMSSDYVRVTQLYCDGVGRQYKDYTQTEGNLEFDICTMWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130612.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130612.1

ORF Insert Sequence:

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TTGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACG TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CAGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130614.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986004.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

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CloneID	OOv13962
Clone ID Related Accession (Same CDS sequence)	XM_012130614.1
Accession Version	XM_012130614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTA GTAAACCTGT TTCTGTCCTG CAAGATTACT GTGATGCCAT TAAAGTCCAC 
ATCTTCTGGC CACTTCTGTT TCAACGTCAG CAGAGCTCCA TAATAACACG GATGCACTCC 
TGTGTAAGGA CCAGCGGTTC TCATGCTTGT GAGATAAGTT TGAAGAAATT ACAGCAGCTT 
GAGCTGATGG AAGATATTGT GGATTTGGCA AAGAAAGTTG TGAATGATAC ATTCTTTATC 
GGAGGCTTAT TGAGAATTGG CTATAAAATA GAGAATAAAA TCTTGGCAAT GGAAGAAGCT 
TTGAATTGGG TGAAATCCAC AGGCGATGTA ACAATTTTAT CTAAATTAGG AGCAGTTGAC 
AATTGTTGGC GTATGTTAAG TATTTTCTTT ACTGAATACA AGTATCACAT AACTAAAATT 
GTAACAGAAA ACTGCAGTTT GCTTGAAGAA TTTAAAACTC AAAGTTGTGC GGAGTGTCTA 
GAGCAAGGAG AACTAATGAA AATGAAAGGA AATGAAGAGT TTTCCAAAGA AAGATATGAT 
ATAGCTATTA TCTATTACTC CAGAGCCATT GAATGTAGGC CTGAAAATCA CCTGCTTTAC 
GGTAACCGAG CTCTTTGTTT TCTTCGTACT GGACAATTCA GAAATGCACT TGGTGATGGA 
AAGAGAGCTA CCATTCTGAA GAACAATTGG ACAAAGGGTC ATTATCGTTA TTGTGCTGCT 
CTTTCTATGC TGGGGGAATA TGACTGGGCC CTGCAAGCAA ACATAAAAGC TCAAAAACTC 
TGTAAAAATG ACCCTGAGGG AATCAAGGAT CTAATTCAGC AGCATGTAAA GTTACAAAAA 
CAGATAGAAG ACCTGCAAGG TCGAACACCA GCTAGTAATC CAGTCAAAGC CTTCTGTGAA 
AGCAGGGCCT ACATACCTAG TTTATCAGCA CCTGCATTTA GTACTTCACT TAACTTTGTG 
GAAACTGAAA AAAATTTCAG AAAAACTAAC CACGAAATGG CAAACGGTGG TAATCAGAAT 
CCAAAGGTGG TAGATGAAGC TCTGAAAGTA GATGATTGTG ACTGTCATCC TGAATTTGTA 
CCACCATCAA GTCAGCCTCC AAAATATAAA GGGAAACAAA AAGCTCGAAA CAATGAATTG 
GAGAAGTTCA GCCCTGGTTC ACAAGAAACT TTACCAGTAG ATTTGAAAAA CATCTTGGAG 
AAACAGTTTT CTAAATCTTC CAGAGCTGCA CACCAGGATT TTGCTAATAT AATGAAAATG 
CTGAGAAGCT TAATCCAGGA TGGCTACACG GCCTTGCTGG AGCAGCGCTG CCGCAGTGCT 
GCCCAGGCCT TCACAGAGTT GCTCAACGGT TTAGATCCTC AAAAAATAAA GCAATTGAAT 
CTGGCCATGA TTAACTACGT GTTAGTAGTC TACGGACTTG CCATTTCACT CCTTGGAATA 
GGACAGCCTG AGGAGTTATC TGAAGCTGAA AACCAATTTA AGAGGATGAT TGAACACTAC 
CCCAATGAGG GACTTGATTG CTTGGCCTAC TGTGGAATTG GAAAAGTATA TTTGAAAAAA 
AACAGATTTT TAGAAGCTCT TAATCATTTT GAGAAAGCAA GAACCTTGAT CTGTCGTCTT 
CCTGGAGTGT TAACTTGGCC CACGAGTAAT GTGATTATTG AGGAGACTCA GCCAGGGAAG 
ATAAAGATGC TGTTAGAAAA ATTCGTTGAA GAATGCAGGT TCCCTCCAGT GCCGGATGCC 
ACTTGTTGCT ATCAGAAGTG CCATGGATAT TCCAAAATTC AGATATATAT AACCGATCCA 
GACTTCAAGG GTTTTATACG AATCAGCTGT TGCCAGTATT GTAAAATAGA ATTTCACATG 
AACTGCTGGA AGAAGTTAAA AACAACAACC TTCAATGATA AAATTGACAA GGATTTTCTG 
CAAGGAATTT GTCTTACTCC TGATTGTAAA GGAATCATTT CAAAGATTAT CATCTTCAGC 
AGTGGTGGTC AAGTTAAATG TGAATTTGAA CACAAGGTCA TTAAAGAAAA GCTTCCTCCA 
AGACCTATTC TGAAACAGAA ATGTTCTAGC CTAGAGAAGT TGAGGCTGAA AGAAGACAAA 
AAATTGAAAC GAAAGCTCCA AAAAAAGGAA GCAAAAAAAT TAGCACAAGA AAGACTGGAA 
GAGGACCTAA GGGAAAGTAA CCCACCAAAA ACTGAAGAGC AGAGAGAAAC TATCAGCCGT 
GTCCAAAGTT GCCAGTTCCT TGATGACAGA ATTCTGCAGT GCATAAAGCA GTACGCTGAC 
AAGATTAAGT CCGGGATACT GAATACTTCC AAGCTTCTCA AAGAATTGCT TTCTTGGAAG 
GTTTTAACCA CAGAAGACTA CACAACGTGT TTTTCTAGCA GAAATTTCCT GAATGAAGCA 
GTGGACTATG TTATTCGTCA TTTGATCCAG GAAAAGAACA GAGTAAAGAC AAGGGTATTT 
CTGCATGTTT TGAGTGAGCT TAAAGAAGTG GAGCCAAAAC TAGCCACCTG GATCCAGAAA 
CTTAATAGCT TTGGCTTAGA TGCCACAGGA CCTTTCTTTT CTCGTTATGG GGCTCCTCTT 
AAGGAGCTTG ATTTTAGCAT CATGACTTTT CTCTGGAATG AGAAATATGG TCATAAGCTA 
GGCTCTATAG AAGGAAAGCA ACTTGATTAT TTCTATGAGC CTACGTCACT GAAGGAAGCG 
CGGTGTTTAA TATGGCTGCT GGAAGAACAC AGAGACAAGT TCCCGGCACT GCATAATGCC 
CTGGACGAGT TCTTTGATAT AATGGACAGC CGCTGCACTG TGTTAAGGAA ACAGGACAGT 
GGTGACGTGC CATGTAATTT TACCAAGATA AAAAACAAAG GCAAGAAAAA GAAGCCAAAA 
GATTCAAAGC CTATGTTAGT TGGATCTGGA ACACCTTCAG TCACACCAAA CAATGAGACT 
TTCACTTCAG GTGAAGACCA TATCAAACGC AGTTTAAATT CTGCGGGCCC CTTTGTAGTG 
CCTGATCACC TTCGGCAGGA TGTAGAAGAA TTTGAAGCTC TGTATGAACA GCATAGTAAT 
GAGTATGTTG TCCGTAATAA GAAGCTGTGG GACATAAACC CAAAACAAAA ATGTTCAACT 
CTATATGATT ATTTCTCTCA GTTGTTGGAG GAACATGGTC CCTTGGACAT GAGTAACAAG 
ATGTTTTCTG AAGAATATGA GTTCTTCCCA GAAGAAACTC GACAGATACT AGAAAAAGCA 
GGAGGTTTGA AAGCGTTTCT CCTCGGATGT CCTCGTTTTG TTGTGATTGA CAACTGTATT 
GCATTGAAGA AAGTTGCATT ACGGCTCAAG AAAAAGAGAA AGAAGAAAAA CATTAAGACA 
AAAGTAGAAG AAATTTCAAA AACAGGAGAG TATTTACGAG TTAAACTACC ACTGAATCCA 
ACTGCTAGGG AATTTAAACC AGATGTAAAG TCCAAACCAG TGTCTGACTT ACCTTTAGCA 
CCAGCTTCTG AGGACGTGAA GCCTCAGCAT GCATCTGCTA ATTCTCCCCA ATCAGCTCGT 
GACACTGGGA AGCCAAAACC AGTTTCCGAT AATTCTTCCA GATCAGTTTC TGAGGATGAG 
AAGCCCAAAG GGATATCTTC TGATTCTCCC AGGCCAGTCC TTGAGGAGAC CATTCACAAG 
CCAGTCTCCT CTAATTCTCC CAAACCAGTT CCCGAGGAAG TGAAAGCGAC CTATTGGCCT 
CGACCCCAGT TGGTCACAGG ATACTGTACA TATCTTCCCT TTCGAGGATT TGACATTACC 
CAGACACCAC CAGCCTTCAT CAGTGTGTTA CCGACTTTGC CCCAGTATAG CATTTACACC 
CCCCTGGCCA GCGTTCCTAC TGAGTATCAC CTGCAAAGAC CAATACCAGT GGTGCCGTCT 
TTTGTAGCCA GTGACATAAC GGAAGTGCAT TTTGAGGCTC ATCATTTCAG TGCTGAGAAT 
GCCCATGGTA ACCAGATTGC CTCCGAAACA CAGATCCTTG AGGAATCTTT GGAAACACCA 
GTGAAGTCAG AGTGCAGCTC TGATGGTGCT GATATAGCCC AGAGTGAGGC TAGCGGAAAC 
GAGGACTACT GTGGAGATTC TGCCAAGTGG GAAGTAAATC CAGAAAGCAC CAATAAAGTA 
ACAAATATTC CATGTACACA AGTGGTTGCT GTACAGGTGT CTTGGAATAT AACACATCAG 
GAAGTCAATA CTGAGCCATA TGGTCCTTTT GAGGCACAAC AGGGAGGTAT TTCACAGATT 
GAAAAGGAGT ACCAGGTTTT ACAAGAGCAG CTTAAGGAAG CATGTGAAAA TTATGAACAC 
AGCAATGTCA GGGGCTCAGA AGAAACCAAG GACTTGGAAG AAAAGTTGAA AAGGAACGTT 
GAAGAAAATA AGATCTCAAA GACAGAACTA GATTGGTTCC TTGAAGATTT GAACAAGGAA 
ATTAAAAAAT GGGAACAGGA GAAAAAAGAA ATCCAAGAAA GACTAAAGGC ACTGAAGAAG 
AAAATGAAAA AGGTGTTGAA TGCCAGCAGA ATGTATACAC AGGAAAACGA TGGAAAGGAA 
AAGGCACTTG AATTACTTCT GGATCAGTCC CTTGAAATCA GTAATACATT TACAAATGAG 
AAAATGAAAA TAGAAGAGAG TATAAAGAAA GGGAAAGAGC ATTATGAAGA GAGTCTTCAG 
AGGGCTCTGG ATGCAGAGGT ATCTGTGCTT GAAAACTGGA AGGATGTGGA GGTGTGTAAG 
TTACAGAGCA TGGAGTCACA GGCCGAAGGG CTTCTCAAGA ACTTGAAGCT AATGAGCAGT 
GATTCGGCCG CATATTCTGA CATGGACTCA GACATACATT CATGGGAATC GTTTCTTTCT 
GATGTTAGAG AAGAAATTGA GAAAGCAAAG TCTCAGTTTG AAGAACAAAT TCAGGCAATT 
AAAAATGGTT CTCGGCTCAG TGAACTTCCT AAAGTGCAGA TTTCTGAGCT TTCGTTTCCT 
ACCTGTAACA CAATTCATCC TAAATTACTT CCTGAGTCTT CAGGCCAGGA GGGTCAAGGT 
CCCTCCACTT CTGCAAGTCA TGGGACCGGA GACCAGACAG CAGCTCCCGA GGAGTCAAGT 
CTGGGTTCTG CTGCTGACAG CCCAGTGGAG TCGCCCTCCT GCAGCCAGAA AGCCCCCCAG 
CTGCCAGAGC GTGAGGGCAC TGGCCAGGCA GCTGGGCCAG AACTAGGCCT GATGGCTGAT 
CAGAAGTTAC CTGTTGCTCC AGAGCGTGCC ACTCGTCCAA GCCAGTCTCC AAAGAAGCCG 
TTCAATAGCA TTATTGAGCA CCTGTCGGTG GTGTTCCCTT GTTACAGCAG CACGGAGCTT 
GCTGGTTTTA TTAAAAAAGT TAGAAACAAA AACAAGACCT CTCTGTCTGG ATTGAGTATT 
GACGAAATTG TGCAAAGAGT GACACAACAC ATCCTTGATG AAGAGAGAAA GAAAAAGCCA 
AATCCAGGAA AGGACCGGAG GCCGTCTGCA CCCAGCTCTG CTGTTCCTGC ACCCAGGGCC 
GCCCAGGACC CGCCCTCCGT GCCTGTCAGA GCATCACCCG AAACCAGGGG GCCGAAGACC 
GAAGACGTCC CTGTGTCCTG TGCCAATTCC TGTGAAATGT GCCAAGAAGC ATTCAAGTCC 
AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AAGGGTGCTT CAAGCAGTGG 
TTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986004.1
CDS	158..5917
Translation	MWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130614.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130614.1

ORF Insert Sequence:

ATGTGGTGTA GTAAACCTGT TTCTGTCCTG CAAGATTACT GTGATGCCAT TAAAGTCCAC 
ATCTTCTGGC CACTTCTGTT TCAACGTCAG CAGAGCTCCA TAATAACACG GATGCACTCC 
TGTGTAAGGA CCAGCGGTTC TCATGCTTGT GAGATAAGTT TGAAGAAATT ACAGCAGCTT 
GAGCTGATGG AAGATATTGT GGATTTGGCA AAGAAAGTTG TGAATGATAC ATTCTTTATC 
GGAGGCTTAT TGAGAATTGG CTATAAAATA GAGAATAAAA TCTTGGCAAT GGAAGAAGCT 
TTGAATTGGG TGAAATCCAC AGGCGATGTA ACAATTTTAT CTAAATTAGG AGCAGTTGAC 
AATTGTTGGC GTATGTTAAG TATTTTCTTT ACTGAATACA AGTATCACAT AACTAAAATT 
GTAACAGAAA ACTGCAGTTT GCTTGAAGAA TTTAAAACTC AAAGTTGTGC GGAGTGTCTA 
GAGCAAGGAG AACTAATGAA AATGAAAGGA AATGAAGAGT TTTCCAAAGA AAGATATGAT 
ATAGCTATTA TCTATTACTC CAGAGCCATT GAATGTAGGC CTGAAAATCA CCTGCTTTAC 
GGTAACCGAG CTCTTTGTTT TCTTCGTACT GGACAATTCA GAAATGCACT TGGTGATGGA 
AAGAGAGCTA CCATTCTGAA GAACAATTGG ACAAAGGGTC ATTATCGTTA TTGTGCTGCT 
CTTTCTATGC TGGGGGAATA TGACTGGGCC CTGCAAGCAA ACATAAAAGC TCAAAAACTC 
TGTAAAAATG ACCCTGAGGG AATCAAGGAT CTAATTCAGC AGCATGTAAA GTTACAAAAA 
CAGATAGAAG ACCTGCAAGG TCGAACACCA GCTAGTAATC CAGTCAAAGC CTTCTGTGAA 
AGCAGGGCCT ACATACCTAG TTTATCAGCA CCTGCATTTA GTACTTCACT TAACTTTGTG 
GAAACTGAAA AAAATTTCAG AAAAACTAAC CACGAAATGG CAAACGGTGG TAATCAGAAT 
CCAAAGGTGG TAGATGAAGC TCTGAAAGTA GATGATTGTG ACTGTCATCC TGAATTTGTA 
CCACCATCAA GTCAGCCTCC AAAATATAAA GGGAAACAAA AAGCTCGAAA CAATGAATTG 
GAGAAGTTCA GCCCTGGTTC ACAAGAAACT TTACCAGTAG ATTTGAAAAA CATCTTGGAG 
AAACAGTTTT CTAAATCTTC CAGAGCTGCA CACCAGGATT TTGCTAATAT AATGAAAATG 
CTGAGAAGCT TAATCCAGGA TGGCTACACG GCCTTGCTGG AGCAGCGCTG CCGCAGTGCT 
GCCCAGGCCT TCACAGAGTT GCTCAACGGT TTAGATCCTC AAAAAATAAA GCAATTGAAT 
CTGGCCATGA TTAACTACGT GTTAGTAGTC TACGGACTTG CCATTTCACT CCTTGGAATA 
GGACAGCCTG AGGAGTTATC TGAAGCTGAA AACCAATTTA AGAGGATGAT TGAACACTAC 
CCCAATGAGG GACTTGATTG CTTGGCCTAC TGTGGAATTG GAAAAGTATA TTTGAAAAAA 
AACAGATTTT TAGAAGCTCT TAATCATTTT GAGAAAGCAA GAACCTTGAT CTGTCGTCTT 
CCTGGAGTGT TAACTTGGCC CACGAGTAAT GTGATTATTG AGGAGACTCA GCCAGGGAAG 
ATAAAGATGC TGTTAGAAAA ATTCGTTGAA GAATGCAGGT TCCCTCCAGT GCCGGATGCC 
ACTTGTTGCT ATCAGAAGTG CCATGGATAT TCCAAAATTC AGATATATAT AACCGATCCA 
GACTTCAAGG GTTTTATACG AATCAGCTGT TGCCAGTATT GTAAAATAGA ATTTCACATG 
AACTGCTGGA AGAAGTTAAA AACAACAACC TTCAATGATA AAATTGACAA GGATTTTCTG 
CAAGGAATTT GTCTTACTCC TGATTGTAAA GGAATCATTT CAAAGATTAT CATCTTCAGC 
AGTGGTGGTC AAGTTAAATG TGAATTTGAA CACAAGGTCA TTAAAGAAAA GCTTCCTCCA 
AGACCTATTC TGAAACAGAA ATGTTCTAGC CTAGAGAAGT TGAGGCTGAA AGAAGACAAA 
AAATTGAAAC GAAAGCTCCA AAAAAAGGAA GCAAAAAAAT TAGCACAAGA AAGACTGGAA 
GAGGACCTAA GGGAAAGTAA CCCACCAAAA ACTGAAGAGC AGAGAGAAAC TATCAGCCGT 
GTCCAAAGTT GCCAGTTCCT TGATGACAGA ATTCTGCAGT GCATAAAGCA GTACGCTGAC 
AAGATTAAGT CCGGGATACT GAATACTTCC AAGCTTCTCA AAGAATTGCT TTCTTGGAAG 
GTTTTAACCA CAGAAGACTA CACAACGTGT TTTTCTAGCA GAAATTTCCT GAATGAAGCA 
GTGGACTATG TTATTCGTCA TTTGATCCAG GAAAAGAACA GAGTAAAGAC AAGGGTATTT 
CTGCATGTTT TGAGTGAGCT TAAAGAAGTG GAGCCAAAAC TAGCCACCTG GATCCAGAAA 
CTTAATAGCT TTGGCTTAGA TGCCACAGGA CCTTTCTTTT CTCGTTATGG GGCTCCTCTT 
AAGGAGCTTG ATTTTAGCAT CATGACTTTT CTCTGGAATG AGAAATATGG TCATAAGCTA 
GGCTCTATAG AAGGAAAGCA ACTTGATTAT TTCTATGAGC CTACGTCACT GAAGGAAGCG 
CGGTGTTTAA TATGGCTGCT GGAAGAACAC AGAGACAAGT TCCCGGCACT GCATAATGCC 
CTGGACGAGT TCTTTGATAT AATGGACAGC CGCTGCACTG TGTTAAGGAA ACAGGACAGT 
GGTGACGTGC CATGTAATTT TACCAAGATA AAAAACAAAG GCAAGAAAAA GAAGCCAAAA 
GATTCAAAGC CTATGTTAGT TGGATCTGGA ACACCTTCAG TCACACCAAA CAATGAGACT 
TTCACTTCAG GTGAAGACCA TATCAAACGC AGTTTAAATT CTGCGGGCCC CTTTGTAGTG 
CCTGATCACC TTCGGCAGGA TGTAGAAGAA TTTGAAGCTC TGTATGAACA GCATAGTAAT 
GAGTATGTTG TCCGTAATAA GAAGCTGTGG GACATAAACC CAAAACAAAA ATGTTCAACT 
CTATATGATT ATTTCTCTCA GTTGTTGGAG GAACATGGTC CCTTGGACAT GAGTAACAAG 
ATGTTTTCTG AAGAATATGA GTTCTTCCCA GAAGAAACTC GACAGATACT AGAAAAAGCA 
GGAGGTTTGA AAGCGTTTCT CCTCGGATGT CCTCGTTTTG TTGTGATTGA CAACTGTATT 
GCATTGAAGA AAGTTGCATT ACGGCTCAAG AAAAAGAGAA AGAAGAAAAA CATTAAGACA 
AAAGTAGAAG AAATTTCAAA AACAGGAGAG TATTTACGAG TTAAACTACC ACTGAATCCA 
ACTGCTAGGG AATTTAAACC AGATGTAAAG TCCAAACCAG TGTCTGACTT ACCTTTAGCA 
CCAGCTTCTG AGGACGTGAA GCCTCAGCAT GCATCTGCTA ATTCTCCCCA ATCAGCTCGT 
GACACTGGGA AGCCAAAACC AGTTTCCGAT AATTCTTCCA GATCAGTTTC TGAGGATGAG 
AAGCCCAAAG GGATATCTTC TGATTCTCCC AGGCCAGTCC TTGAGGAGAC CATTCACAAG 
CCAGTCTCCT CTAATTCTCC CAAACCAGTT CCCGAGGAAG TGAAAGCGAC CTATTGGCCT 
CGACCCCAGT TGGTCACAGG ATACTGTACA TATCTTCCCT TTCGAGGATT TGACATTACC 
CAGACACCAC CAGCCTTCAT CAGTGTGTTA CCGACTTTGC CCCAGTATAG CATTTACACC 
CCCCTGGCCA GCGTTCCTAC TGAGTATCAC CTGCAAAGAC CAATACCAGT GGTGCCGTCT 
TTTGTAGCCA GTGACATAAC GGAAGTGCAT TTTGAGGCTC ATCATTTCAG TGCTGAGAAT 
GCCCATGGTA ACCAGATTGC CTCCGAAACA CAGATCCTTG AGGAATCTTT GGAAACACCA 
GTGAAGTCAG AGTGCAGCTC TGATGGTGCT GATATAGCCC AGAGTGAGGC TAGCGGAAAC 
GAGGACTACT GTGGAGATTC TGCCAAGTGG GAAGTAAATC CAGAAAGCAC CAATAAAGTA 
ACAAATATTC CATGTACACA AGTGGTTGCT GTACAGGTGT CTTGGAATAT AACACATCAG 
GAAGTCAATA CTGAGCCATA TGGTCCTTTT GAGGCACAAC AGGGAGGTAT TTCACAGATT 
GAAAAGGAGT ACCAGGTTTT ACAAGAGCAG CTTAAGGAAG CATGTGAAAA TTATGAACAC 
AGCAATGTCA GGGGCTCAGA AGAAACCAAG GACTTGGAAG AAAAGTTGAA AAGGAACGTT 
GAAGAAAATA AGATCTCAAA GACAGAACTA GATTGGTTCC TTGAAGATTT GAACAAGGAA 
ATTAAAAAAT GGGAACAGGA GAAAAAAGAA ATCCAAGAAA GACTAAAGGC ACTGAAGAAG 
AAAATGAAAA AGGTGTTGAA TGCCAGCAGA ATGTATACAC AGGAAAACGA TGGAAAGGAA 
AAGGCACTTG AATTACTTCT GGATCAGTCC CTTGAAATCA GTAATACATT TACAAATGAG 
AAAATGAAAA TAGAAGAGAG TATAAAGAAA GGGAAAGAGC ATTATGAAGA GAGTCTTCAG 
AGGGCTCTGG ATGCAGAGGT ATCTGTGCTT GAAAACTGGA AGGATGTGGA GGTGTGTAAG 
TTACAGAGCA TGGAGTCACA GGCCGAAGGG CTTCTCAAGA ACTTGAAGCT AATGAGCAGT 
GATTCGGCCG CATATTCTGA CATGGACTCA GACATACATT CATGGGAATC GTTTCTTTCT 
GATGTTAGAG AAGAAATTGA GAAAGCAAAG TCTCAGTTTG AAGAACAAAT TCAGGCAATT 
AAAAATGGTT CTCGGCTCAG TGAACTTCCT AAAGTGCAGA TTTCTGAGCT TTCGTTTCCT 
ACCTGTAACA CAATTCATCC TAAATTACTT CCTGAGTCTT CAGGCCAGGA GGGTCAAGGT 
CCCTCCACTT CTGCAAGTCA TGGGACCGGA GACCAGACAG CAGCTCCCGA GGAGTCAAGT 
CTGGGTTCTG CTGCTGACAG CCCAGTGGAG TCGCCCTCCT GCAGCCAGAA AGCCCCCCAG 
CTGCCAGAGC GTGAGGGCAC TGGCCAGGCA GCTGGGCCAG AACTAGGCCT GATGGCTGAT 
CAGAAGTTAC CTGTTGCTCC AGAGCGTGCC ACTCGTCCAA GCCAGTCTCC AAAGAAGCCG 
TTCAATAGCA TTATTGAGCA CCTGTCGGTG GTGTTCCCTT GTTACAGCAG CACGGAGCTT 
GCTGGTTTTA TTAAAAAAGT TAGAAACAAA AACAAGACCT CTCTGTCTGG ATTGAGTATT 
GACGAAATTG TGCAAAGAGT GACACAACAC ATCCTTGATG AAGAGAGAAA GAAAAAGCCA 
AATCCAGGAA AGGACCGGAG GCCGTCTGCA CCCAGCTCTG CTGTTCCTGC ACCCAGGGCC 
GCCCAGGACC CGCCCTCCGT GCCTGTCAGA GCATCACCCG AAACCAGGGG GCCGAAGACC 
GAAGACGTCC CTGTGTCCTG TGCCAATTCC TGTGAAATGT GCCAAGAAGC ATTCAAGTCC 
AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AAGGGTGCTT CAAGCAGTGG 
TTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130615.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986005.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OOv13963
Clone ID Related Accession (Same CDS sequence)	XM_012130615.2 , XM_012130616.2
Accession Version	XM_012130615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130615.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC ACGTTAGAGG CTGGGATCCT CAGAATAAAG CTGCAAATGC ACTTGGTGAT 
GGAAAGAGAG CTACCATTCT GAAGAACAAT TGGACAAAGG GTCATTATCG TTATTGTGCT 
GCTCTTTCTA TGCTGGGGGA ATATGACTGG GCCCTGCAAG CAAACATAAA AGCTCAAAAA 
CTCTGTAAAA ATGACCCTGA GGGAATCAAG GATCTAATTC AGCAGCATGT AAAGTTACAA 
AAACAGATAG AAGACCTGCA AGGTCGAACA CCAGCTAGTA ATCCAGTCAA AGCCTTCTGT 
GAAAGCAGGG CCTACATACC TAGTTTATCA GCACCTGCAT TTAGTACTTC ACTTAACTTT 
GTGGAAACTG AAAAAAATTT CAGAAAAACT AACCACGAAA TGGCAAACGG TGGTAATCAG 
AATCCAAAGG TGGTAGATGA AGCTCTGAAA GTAGATGATT GTGACTGTCA TCCTGAATTT 
GTACCACCAT CAAGTCAGCC TCCAAAATAT AAAGGGAAAC AAAAAGCTCG AAACAATGAA 
TTGGAGAAGT TCAGCCCTGG TTCACAAGAA ACTTTACCAG TAGATTTGAA AAACATCTTG 
GAGAAACAGT TTTCTAAATC TTCCAGAGCT GCACACCAGG ATTTTGCTAA TATAATGAAA 
ATGCTGAGAA GCTTAATCCA GGATGGCTAC ACGGCCTTGC TGGAGCAGCG CTGCCGCAGT 
GCTGCCCAGG CCTTCACAGA GTTGCTCAAC GGTTTAGATC CTCAAAAAAT AAAGCAATTG 
AATCTGGCCA TGATTAACTA CGTGTTAGTA GTCTACGGAC TTGCCATTTC ACTCCTTGGA 
ATAGGACAGC CTGAGGAGTT ATCTGAAGCT GAAAACCAAT TTAAGAGGAT GATTGAACAC 
TACCCCAATG AGGGACTTGA TTGCTTGGCC TACTGTGGAA TTGGAAAAGT ATATTTGAAA 
AAAAACAGAT TTTTAGAAGC TCTTAATCAT TTTGAGAAAG CAAGAACCTT GATCTGTCGT 
CTTCCTGGAG TGTTAACTTG GCCCACGAGT AATGTGATTA TTGAGGAGAC TCAGCCAGGG 
AAGATAAAGA TGCTGTTAGA AAAATTCGTT GAAGAATGCA GGTTCCCTCC AGTGCCGGAT 
GCCACTTGTT GCTATCAGAA GTGCCATGGA TATTCCAAAA TTCAGATATA TATAACCGAT 
CCAGACTTCA AGGGTTTTAT ACGAATCAGC TGTTGCCAGT ATTGTAAAAT AGAATTTCAC 
ATGAACTGCT GGAAGAAGTT AAAAACAACA ACCTTCAATG ATAAAATTGA CAAGGATTTT 
CTGCAAGGAA TTTGTCTTAC TCCTGATTGT AAAGGAATCA TTTCAAAGAT TATCATCTTC 
AGCAGTGGTG GTCAAGTTAA ATGTGAATTT GAACACAAGG TCATTAAAGA AAAGCTTCCT 
CCAAGACCTA TTCTGAAACA GAAATGTTCT AGCCTAGAGA AGTTGAGGCT GAAAGAAGAC 
AAAAAATTGA AACGAAAGCT CCAAAAAAAG GAAGCAAAAA AATTAGCACA AGAAAGACTG 
GAAGAGGACC TAAGGGAAAG TAACCCACCA AAAACTGAAG AGCAGAGAGA AACTATCAGC 
CGTGTCCAAA GTTGCCAGTT CCTTGATGAC AGAATTCTGC AGTGCATAAA GCAGTACGCT 
GACAAGATTA AGTCCGGGAT ACTGAATACT TCCAAGCTTC TCAAAGAATT GCTTTCTTGG 
AAGGTTTTAA CCACAGAAGA CTACACAACG TGTTTTTCTA GCAGAAATTT CCTGAATGAA 
GCAGTGGACT ATGTTATTCG TCATTTGATC CAGGAAAAGA ACAGAGTAAA GACAAGGGTA 
TTTCTGCATG TTTTGAGTGA GCTTAAAGAA GTGGAGCCAA AACTAGCCAC CTGGATCCAG 
AAACTTAATA GCTTTGGCTT AGATGCCACA GGACCTTTCT TTTCTCGTTA TGGGGCTCCT 
CTTAAGGAGC TTGATTTTAG CATCATGACT TTTCTCTGGA ATGAGAAATA TGGTCATAAG 
CTAGGCTCTA TAGAAGGAAA GCAACTTGAT TATTTCTATG AGCCTACGTC ACTGAAGGAA 
GCGCGGTGTT TAATATGGCT GCTGGAAGAA CACAGAGACA AGTTCCCGGC ACTGCATAAT 
GCCCTGGACG AGTTCTTTGA TATAATGGAC AGCCGCTGCA CTGTGTTAAG GAAACAGGAC 
AGTGGTGACG TGCCATGTAA TTTTACCAAG ATAAAAAACA AAGGCAAGAA AAAGAAGCCA 
AAAGATTCAA AGCCTATGTT AGTTGGATCT GGAACACCTT CAGTCACACC AAACAATGAG 
ACTTTCACTT CAGGTGAAGA CCATATCAAA CGCAGTTTAA ATTCTGCGGG CCCCTTTGTA 
GTGCCTGATC ACCTTCGGCA GGATGTAGAA GAATTTGAAG CTCTGTATGA ACAGCATAGT 
AATGAGTATG TTGTCCGTAA TAAGAAGCTG TGGGACATAA ACCCAAAACA AAAATGTTCA 
ACTCTATATG ATTATTTCTC TCAGTTGTTG GAGGAACATG GTCCCTTGGA CATGAGTAAC 
AAGATGTTTT CTGAAGAATA TGAGTTCTTC CCAGAAGAAA CTCGACAGAT ACTAGAAAAA 
GCAGGAGGTT TGAAAGCGTT TCTCCTCGGA TGTCCTCGTT TTGTTGTGAT TGACAACTGT 
ATTGCATTGA AGAAAGTTGC ATTACGGCTC AAGAAAAAGA GAAAGAAGAA AAACATTAAG 
ACAAAAGTAG AAGAAATTTC AAAAACAGGA GAGTATTTAC GAGTTAAACT ACCACTGAAT 
CCAACTGCTA GGGAATTTAA ACCAGATGTA AAGTCCAAAC CAGTGTCTGA CTTACCTTTA 
GCACCAGCTT CTGAGGACGT GAAGCCTCAG CATGCATCTG CTAATTCTCC CCAATCAGCT 
CGTGACACTG GGAAGCCAAA ACCAGTTTCC GATAATTCTT CCAGATCAGT TTCTGAGGAT 
GAGAAGCCCA AAGGGATATC TTCTGATTCT CCCAGGCCAG TCCTTGAGGA GACCATTCAC 
AAGCCAGTCT CCTCTAATTC TCCCAAACCA GTTCCCGAGG AAGTGAAAGC GACCTATTGG 
CCTCGACCCC AGTTGGTCAC AGGATACTGT ACATATCTTC CCTTTCGAGG ATTTGACATT 
ACCCAGACAC CACCAGCCTT CATCAGTGTG TTACCGACTT TGCCCCAGTA TAGCATTTAC 
ACCCCCCTGG CCAGCGTTCC TACTGAGTAT CACCTGCAAA GACCAATACC AGTGGTGCCG 
TCTTTTGTAG CCAGTGACAT AACGGAAGTG CATTTTGAGG CTCATCATTT CAGTGCTGAG 
AATGCCCATG GTAACCAGAT TGCCTCCGAA ACACAGATCC TTGAGGAATC TTTGGAAACA 
CCAGTGAAGT CAGAGTGCAG CTCTGATGGT GCTGATATAG CCCAGAGTGA GGCTAGCGGA 
AACGAGGACT ACTGTGGAGA TTCTGCCAAG TGGGAAGTAA ATCCAGAAAG CACCAATAAA 
GTAACAAATA TTCCATGTAC ACAAGTGGTT GCTGTACAGG TGTCTTGGAA TATAACACAT 
CAGGAAGTCA ATACTGAGCC ATATGGTCCT TTTGAGGCAC AACAGGGAGG TATTTCACAG 
ATTGAAAAGG AGTACCAGGT TTTACAAGAG CAGCTTAAGG AAGCATGTGA AAATTATGAA 
CACAGCAATG TCAGGGGCTC AGAAGAAACC AAGGACTTGG AAGAAAAGTT GAAAAGGAAC 
GTTGAAGAAA ATAAGATCTC AAAGACAGAA CTAGATTGGT TCCTTGAAGA TTTGAACAAG 
GAAATTAAAA AATGGGAACA GGAGAAAAAA GAAATCCAAG AAAGACTAAA GGCACTGAAG 
AAGAAAATGA AAAAGGTGTT GAATGCCAGC AGAATGTATA CACAGGAAAA CGATGGAAAG 
GAAAAGGCAC TTGAATTACT TCTGGATCAG TCCCTTGAAA TCAGTAATAC ATTTACAAAT 
GAGAAAATGA AAATAGAAGA GAGTATAAAG AAAGGGAAAG AGCATTATGA AGAGAGTCTT 
CAGAGGGCTC TGGATGCAGA GGTATCTGTG CTTGAAAACT GGAAGGATGT GGAGGTGTGT 
AAGTTACAGA GCATGGAGTC ACAGGCCGAA GGGCTTCTCA AGAACTTGAA GCTAATGAGC 
AGTGATTCGG CCGCATATTC TGACATGGAC TCAGACATAC ATTCATGGGA ATCGTTTCTT 
TCTGATGTTA GAGAAGAAAT TGAGAAAGCA AAGTCTCAGT TTGAAGAACA AATTCAGGCA 
ATTAAAAATG GTTCTCGGCT CAGTGAACTT CCTAAAGTGC AGATTTCTGA GCTTTCGTTT 
CCTACCTGTA ACACAATTCA TCCTAAATTA CTTCCTGAGT CTTCAGGCCA GGAGGGTCAA 
GGTCCCTCCA CTTCTGCAAG TCATGGGACC GGAGACCAGA CAGCAGCTCC CGAGGAGTCA 
AGTCTGGGTT CTGCTGCTGA CAGCCCAGTG GAGTCGCCCT CCTGCAGCCA GAAAGCCCCC 
CAGCTGCCAG AGCGTGAGGG CACTGGCCAG GCAGCTGGGC CAGAACTAGG CCTGATGGCT 
GATCAGAAGT TACCTGTTGC TCCAGAGCGT GCCACTCGTC CAAGCCAGTC TCCAAAGAAG 
CCGTTCAATA GCATTATTGA GCACCTGTCG GTGGTGTTCC CTTGTTACAG CAGCACGGAG 
CTTGCTGGTT TTATTAAAAA AGTTAGAAAC AAAAACAAGA CCTCTCTGTC TGGATTGAGT 
ATTGACGAAA TTGTGCAAAG AGTGACACAA CACATCCTTG ATGAAGAGAG AAAGAAAAAG 
CCAAATCCAG GAAAGGACCG GAGGCCGTCT GCACCCAGCT CTGCTGTTCC TGCACCCAGG 
GCCGCCCAGG ACCCGCCCTC CGTGCCTGTC AGAGCATCAC CCGAAACCAG GGGGCCGAAG 
ACCGAAGACG TCCCTGTGTC CTGTGCCAAT TCCTGTGAAA TGTGCCAAGA AGCATTCAAG 
TCCAAACACG TGCGTGAGCT GCAGTGTGGG CACAGGCTTC ACAAAGGGTG CTTCAAGCAG 
TGGTTGAAGA GCCAGCGCAC CTGCCCAGCC TGCCTGGGTC ACGACCTCCT GTCAGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986005.1
CDS	1004..6166
Translation	MKKHVRGWDPQNKAANALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130615.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130615.2

ORF Insert Sequence:

ATGAAAAAAC ACGTTAGAGG CTGGGATCCT CAGAATAAAG CTGCAAATGC ACTTGGTGAT 
GGAAAGAGAG CTACCATTCT GAAGAACAAT TGGACAAAGG GTCATTATCG TTATTGTGCT 
GCTCTTTCTA TGCTGGGGGA ATATGACTGG GCCCTGCAAG CAAACATAAA AGCTCAAAAA 
CTCTGTAAAA ATGACCCTGA GGGAATCAAG GATCTAATTC AGCAGCATGT AAAGTTACAA 
AAACAGATAG AAGACCTGCA AGGTCGAACA CCAGCTAGTA ATCCAGTCAA AGCCTTCTGT 
GAAAGCAGGG CCTACATACC TAGTTTATCA GCACCTGCAT TTAGTACTTC ACTTAACTTT 
GTGGAAACTG AAAAAAATTT CAGAAAAACT AACCACGAAA TGGCAAACGG TGGTAATCAG 
AATCCAAAGG TGGTAGATGA AGCTCTGAAA GTAGATGATT GTGACTGTCA TCCTGAATTT 
GTACCACCAT CAAGTCAGCC TCCAAAATAT AAAGGGAAAC AAAAAGCTCG AAACAATGAA 
TTGGAGAAGT TCAGCCCTGG TTCACAAGAA ACTTTACCAG TAGATTTGAA AAACATCTTG 
GAGAAACAGT TTTCTAAATC TTCCAGAGCT GCACACCAGG ATTTTGCTAA TATAATGAAA 
ATGCTGAGAA GCTTAATCCA GGATGGCTAC ACGGCCTTGC TGGAGCAGCG CTGCCGCAGT 
GCTGCCCAGG CCTTCACAGA GTTGCTCAAC GGTTTAGATC CTCAAAAAAT AAAGCAATTG 
AATCTGGCCA TGATTAACTA CGTGTTAGTA GTCTACGGAC TTGCCATTTC ACTCCTTGGA 
ATAGGACAGC CTGAGGAGTT ATCTGAAGCT GAAAACCAAT TTAAGAGGAT GATTGAACAC 
TACCCCAATG AGGGACTTGA TTGCTTGGCC TACTGTGGAA TTGGAAAAGT ATATTTGAAA 
AAAAACAGAT TTTTAGAAGC TCTTAATCAT TTTGAGAAAG CAAGAACCTT GATCTGTCGT 
CTTCCTGGAG TGTTAACTTG GCCCACGAGT AATGTGATTA TTGAGGAGAC TCAGCCAGGG 
AAGATAAAGA TGCTGTTAGA AAAATTCGTT GAAGAATGCA GGTTCCCTCC AGTGCCGGAT 
GCCACTTGTT GCTATCAGAA GTGCCATGGA TATTCCAAAA TTCAGATATA TATAACCGAT 
CCAGACTTCA AGGGTTTTAT ACGAATCAGC TGTTGCCAGT ATTGTAAAAT AGAATTTCAC 
ATGAACTGCT GGAAGAAGTT AAAAACAACA ACCTTCAATG ATAAAATTGA CAAGGATTTT 
CTGCAAGGAA TTTGTCTTAC TCCTGATTGT AAAGGAATCA TTTCAAAGAT TATCATCTTC 
AGCAGTGGTG GTCAAGTTAA ATGTGAATTT GAACACAAGG TCATTAAAGA AAAGCTTCCT 
CCAAGACCTA TTCTGAAACA GAAATGTTCT AGCCTAGAGA AGTTGAGGCT GAAAGAAGAC 
AAAAAATTGA AACGAAAGCT CCAAAAAAAG GAAGCAAAAA AATTAGCACA AGAAAGACTG 
GAAGAGGACC TAAGGGAAAG TAACCCACCA AAAACTGAAG AGCAGAGAGA AACTATCAGC 
CGTGTCCAAA GTTGCCAGTT CCTTGATGAC AGAATTCTGC AGTGCATAAA GCAGTACGCT 
GACAAGATTA AGTCCGGGAT ACTGAATACT TCCAAGCTTC TCAAAGAATT GCTTTCTTGG 
AAGGTTTTAA CCACAGAAGA CTACACAACG TGTTTTTCTA GCAGAAATTT CCTGAATGAA 
GCAGTGGACT ATGTTATTCG TCATTTGATC CAGGAAAAGA ACAGAGTAAA GACAAGGGTA 
TTTCTGCATG TTTTGAGTGA GCTTAAAGAA GTGGAGCCAA AACTAGCCAC CTGGATCCAG 
AAACTTAATA GCTTTGGCTT AGATGCCACA GGACCTTTCT TTTCTCGTTA TGGGGCTCCT 
CTTAAGGAGC TTGATTTTAG CATCATGACT TTTCTCTGGA ATGAGAAATA TGGTCATAAG 
CTAGGCTCTA TAGAAGGAAA GCAACTTGAT TATTTCTATG AGCCTACGTC ACTGAAGGAA 
GCGCGGTGTT TAATATGGCT GCTGGAAGAA CACAGAGACA AGTTCCCGGC ACTGCATAAT 
GCCCTGGACG AGTTCTTTGA TATAATGGAC AGCCGCTGCA CTGTGTTAAG GAAACAGGAC 
AGTGGTGACG TGCCATGTAA TTTTACCAAG ATAAAAAACA AAGGCAAGAA AAAGAAGCCA 
AAAGATTCAA AGCCTATGTT AGTTGGATCT GGAACACCTT CAGTCACACC AAACAATGAG 
ACTTTCACTT CAGGTGAAGA CCATATCAAA CGCAGTTTAA ATTCTGCGGG CCCCTTTGTA 
GTGCCTGATC ACCTTCGGCA GGATGTAGAA GAATTTGAAG CTCTGTATGA ACAGCATAGT 
AATGAGTATG TTGTCCGTAA TAAGAAGCTG TGGGACATAA ACCCAAAACA AAAATGTTCA 
ACTCTATATG ATTATTTCTC TCAGTTGTTG GAGGAACATG GTCCCTTGGA CATGAGTAAC 
AAGATGTTTT CTGAAGAATA TGAGTTCTTC CCAGAAGAAA CTCGACAGAT ACTAGAAAAA 
GCAGGAGGTT TGAAAGCGTT TCTCCTCGGA TGTCCTCGTT TTGTTGTGAT TGACAACTGT 
ATTGCATTGA AGAAAGTTGC ATTACGGCTC AAGAAAAAGA GAAAGAAGAA AAACATTAAG 
ACAAAAGTAG AAGAAATTTC AAAAACAGGA GAGTATTTAC GAGTTAAACT ACCACTGAAT 
CCAACTGCTA GGGAATTTAA ACCAGATGTA AAGTCCAAAC CAGTGTCTGA CTTACCTTTA 
GCACCAGCTT CTGAGGACGT GAAGCCTCAG CATGCATCTG CTAATTCTCC CCAATCAGCT 
CGTGACACTG GGAAGCCAAA ACCAGTTTCC GATAATTCTT CCAGATCAGT TTCTGAGGAT 
GAGAAGCCCA AAGGGATATC TTCTGATTCT CCCAGGCCAG TCCTTGAGGA GACCATTCAC 
AAGCCAGTCT CCTCTAATTC TCCCAAACCA GTTCCCGAGG AAGTGAAAGC GACCTATTGG 
CCTCGACCCC AGTTGGTCAC AGGATACTGT ACATATCTTC CCTTTCGAGG ATTTGACATT 
ACCCAGACAC CACCAGCCTT CATCAGTGTG TTACCGACTT TGCCCCAGTA TAGCATTTAC 
ACCCCCCTGG CCAGCGTTCC TACTGAGTAT CACCTGCAAA GACCAATACC AGTGGTGCCG 
TCTTTTGTAG CCAGTGACAT AACGGAAGTG CATTTTGAGG CTCATCATTT CAGTGCTGAG 
AATGCCCATG GTAACCAGAT TGCCTCCGAA ACACAGATCC TTGAGGAATC TTTGGAAACA 
CCAGTGAAGT CAGAGTGCAG CTCTGATGGT GCTGATATAG CCCAGAGTGA GGCTAGCGGA 
AACGAGGACT ACTGTGGAGA TTCTGCCAAG TGGGAAGTAA ATCCAGAAAG CACCAATAAA 
GTAACAAATA TTCCATGTAC ACAAGTGGTT GCTGTACAGG TGTCTTGGAA TATAACACAT 
CAGGAAGTCA ATACTGAGCC ATATGGTCCT TTTGAGGCAC AACAGGGAGG TATTTCACAG 
ATTGAAAAGG AGTACCAGGT TTTACAAGAG CAGCTTAAGG AAGCATGTGA AAATTATGAA 
CACAGCAATG TCAGGGGCTC AGAAGAAACC AAGGACTTGG AAGAAAAGTT GAAAAGGAAC 
GTTGAAGAAA ATAAGATCTC AAAGACAGAA CTAGATTGGT TCCTTGAAGA TTTGAACAAG 
GAAATTAAAA AATGGGAACA GGAGAAAAAA GAAATCCAAG AAAGACTAAA GGCACTGAAG 
AAGAAAATGA AAAAGGTGTT GAATGCCAGC AGAATGTATA CACAGGAAAA CGATGGAAAG 
GAAAAGGCAC TTGAATTACT TCTGGATCAG TCCCTTGAAA TCAGTAATAC ATTTACAAAT 
GAGAAAATGA AAATAGAAGA GAGTATAAAG AAAGGGAAAG AGCATTATGA AGAGAGTCTT 
CAGAGGGCTC TGGATGCAGA GGTATCTGTG CTTGAAAACT GGAAGGATGT GGAGGTGTGT 
AAGTTACAGA GCATGGAGTC ACAGGCCGAA GGGCTTCTCA AGAACTTGAA GCTAATGAGC 
AGTGATTCGG CCGCATATTC TGACATGGAC TCAGACATAC ATTCATGGGA ATCGTTTCTT 
TCTGATGTTA GAGAAGAAAT TGAGAAAGCA AAGTCTCAGT TTGAAGAACA AATTCAGGCA 
ATTAAAAATG GTTCTCGGCT CAGTGAACTT CCTAAAGTGC AGATTTCTGA GCTTTCGTTT 
CCTACCTGTA ACACAATTCA TCCTAAATTA CTTCCTGAGT CTTCAGGCCA GGAGGGTCAA 
GGTCCCTCCA CTTCTGCAAG TCATGGGACC GGAGACCAGA CAGCAGCTCC CGAGGAGTCA 
AGTCTGGGTT CTGCTGCTGA CAGCCCAGTG GAGTCGCCCT CCTGCAGCCA GAAAGCCCCC 
CAGCTGCCAG AGCGTGAGGG CACTGGCCAG GCAGCTGGGC CAGAACTAGG CCTGATGGCT 
GATCAGAAGT TACCTGTTGC TCCAGAGCGT GCCACTCGTC CAAGCCAGTC TCCAAAGAAG 
CCGTTCAATA GCATTATTGA GCACCTGTCG GTGGTGTTCC CTTGTTACAG CAGCACGGAG 
CTTGCTGGTT TTATTAAAAA AGTTAGAAAC AAAAACAAGA CCTCTCTGTC TGGATTGAGT 
ATTGACGAAA TTGTGCAAAG AGTGACACAA CACATCCTTG ATGAAGAGAG AAAGAAAAAG 
CCAAATCCAG GAAAGGACCG GAGGCCGTCT GCACCCAGCT CTGCTGTTCC TGCACCCAGG 
GCCGCCCAGG ACCCGCCCTC CGTGCCTGTC AGAGCATCAC CCGAAACCAG GGGGCCGAAG 
ACCGAAGACG TCCCTGTGTC CTGTGCCAAT TCCTGTGAAA TGTGCCAAGA AGCATTCAAG 
TCCAAACACG TGCGTGAGCT GCAGTGTGGG CACAGGCTTC ACAAAGGGTG CTTCAAGCAG 
TGGTTGAAGA GCCAGCGCAC CTGCCCAGCC TGCCTGGGTC ACGACCTCCT GTCAGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130616.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986006.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OOv13963
Clone ID Related Accession (Same CDS sequence)	XM_012130615.2 , XM_012130616.2
Accession Version	XM_012130616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130616.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC ACGTTAGAGG CTGGGATCCT CAGAATAAAG CTGCAAATGC ACTTGGTGAT 
GGAAAGAGAG CTACCATTCT GAAGAACAAT TGGACAAAGG GTCATTATCG TTATTGTGCT 
GCTCTTTCTA TGCTGGGGGA ATATGACTGG GCCCTGCAAG CAAACATAAA AGCTCAAAAA 
CTCTGTAAAA ATGACCCTGA GGGAATCAAG GATCTAATTC AGCAGCATGT AAAGTTACAA 
AAACAGATAG AAGACCTGCA AGGTCGAACA CCAGCTAGTA ATCCAGTCAA AGCCTTCTGT 
GAAAGCAGGG CCTACATACC TAGTTTATCA GCACCTGCAT TTAGTACTTC ACTTAACTTT 
GTGGAAACTG AAAAAAATTT CAGAAAAACT AACCACGAAA TGGCAAACGG TGGTAATCAG 
AATCCAAAGG TGGTAGATGA AGCTCTGAAA GTAGATGATT GTGACTGTCA TCCTGAATTT 
GTACCACCAT CAAGTCAGCC TCCAAAATAT AAAGGGAAAC AAAAAGCTCG AAACAATGAA 
TTGGAGAAGT TCAGCCCTGG TTCACAAGAA ACTTTACCAG TAGATTTGAA AAACATCTTG 
GAGAAACAGT TTTCTAAATC TTCCAGAGCT GCACACCAGG ATTTTGCTAA TATAATGAAA 
ATGCTGAGAA GCTTAATCCA GGATGGCTAC ACGGCCTTGC TGGAGCAGCG CTGCCGCAGT 
GCTGCCCAGG CCTTCACAGA GTTGCTCAAC GGTTTAGATC CTCAAAAAAT AAAGCAATTG 
AATCTGGCCA TGATTAACTA CGTGTTAGTA GTCTACGGAC TTGCCATTTC ACTCCTTGGA 
ATAGGACAGC CTGAGGAGTT ATCTGAAGCT GAAAACCAAT TTAAGAGGAT GATTGAACAC 
TACCCCAATG AGGGACTTGA TTGCTTGGCC TACTGTGGAA TTGGAAAAGT ATATTTGAAA 
AAAAACAGAT TTTTAGAAGC TCTTAATCAT TTTGAGAAAG CAAGAACCTT GATCTGTCGT 
CTTCCTGGAG TGTTAACTTG GCCCACGAGT AATGTGATTA TTGAGGAGAC TCAGCCAGGG 
AAGATAAAGA TGCTGTTAGA AAAATTCGTT GAAGAATGCA GGTTCCCTCC AGTGCCGGAT 
GCCACTTGTT GCTATCAGAA GTGCCATGGA TATTCCAAAA TTCAGATATA TATAACCGAT 
CCAGACTTCA AGGGTTTTAT ACGAATCAGC TGTTGCCAGT ATTGTAAAAT AGAATTTCAC 
ATGAACTGCT GGAAGAAGTT AAAAACAACA ACCTTCAATG ATAAAATTGA CAAGGATTTT 
CTGCAAGGAA TTTGTCTTAC TCCTGATTGT AAAGGAATCA TTTCAAAGAT TATCATCTTC 
AGCAGTGGTG GTCAAGTTAA ATGTGAATTT GAACACAAGG TCATTAAAGA AAAGCTTCCT 
CCAAGACCTA TTCTGAAACA GAAATGTTCT AGCCTAGAGA AGTTGAGGCT GAAAGAAGAC 
AAAAAATTGA AACGAAAGCT CCAAAAAAAG GAAGCAAAAA AATTAGCACA AGAAAGACTG 
GAAGAGGACC TAAGGGAAAG TAACCCACCA AAAACTGAAG AGCAGAGAGA AACTATCAGC 
CGTGTCCAAA GTTGCCAGTT CCTTGATGAC AGAATTCTGC AGTGCATAAA GCAGTACGCT 
GACAAGATTA AGTCCGGGAT ACTGAATACT TCCAAGCTTC TCAAAGAATT GCTTTCTTGG 
AAGGTTTTAA CCACAGAAGA CTACACAACG TGTTTTTCTA GCAGAAATTT CCTGAATGAA 
GCAGTGGACT ATGTTATTCG TCATTTGATC CAGGAAAAGA ACAGAGTAAA GACAAGGGTA 
TTTCTGCATG TTTTGAGTGA GCTTAAAGAA GTGGAGCCAA AACTAGCCAC CTGGATCCAG 
AAACTTAATA GCTTTGGCTT AGATGCCACA GGACCTTTCT TTTCTCGTTA TGGGGCTCCT 
CTTAAGGAGC TTGATTTTAG CATCATGACT TTTCTCTGGA ATGAGAAATA TGGTCATAAG 
CTAGGCTCTA TAGAAGGAAA GCAACTTGAT TATTTCTATG AGCCTACGTC ACTGAAGGAA 
GCGCGGTGTT TAATATGGCT GCTGGAAGAA CACAGAGACA AGTTCCCGGC ACTGCATAAT 
GCCCTGGACG AGTTCTTTGA TATAATGGAC AGCCGCTGCA CTGTGTTAAG GAAACAGGAC 
AGTGGTGACG TGCCATGTAA TTTTACCAAG ATAAAAAACA AAGGCAAGAA AAAGAAGCCA 
AAAGATTCAA AGCCTATGTT AGTTGGATCT GGAACACCTT CAGTCACACC AAACAATGAG 
ACTTTCACTT CAGGTGAAGA CCATATCAAA CGCAGTTTAA ATTCTGCGGG CCCCTTTGTA 
GTGCCTGATC ACCTTCGGCA GGATGTAGAA GAATTTGAAG CTCTGTATGA ACAGCATAGT 
AATGAGTATG TTGTCCGTAA TAAGAAGCTG TGGGACATAA ACCCAAAACA AAAATGTTCA 
ACTCTATATG ATTATTTCTC TCAGTTGTTG GAGGAACATG GTCCCTTGGA CATGAGTAAC 
AAGATGTTTT CTGAAGAATA TGAGTTCTTC CCAGAAGAAA CTCGACAGAT ACTAGAAAAA 
GCAGGAGGTT TGAAAGCGTT TCTCCTCGGA TGTCCTCGTT TTGTTGTGAT TGACAACTGT 
ATTGCATTGA AGAAAGTTGC ATTACGGCTC AAGAAAAAGA GAAAGAAGAA AAACATTAAG 
ACAAAAGTAG AAGAAATTTC AAAAACAGGA GAGTATTTAC GAGTTAAACT ACCACTGAAT 
CCAACTGCTA GGGAATTTAA ACCAGATGTA AAGTCCAAAC CAGTGTCTGA CTTACCTTTA 
GCACCAGCTT CTGAGGACGT GAAGCCTCAG CATGCATCTG CTAATTCTCC CCAATCAGCT 
CGTGACACTG GGAAGCCAAA ACCAGTTTCC GATAATTCTT CCAGATCAGT TTCTGAGGAT 
GAGAAGCCCA AAGGGATATC TTCTGATTCT CCCAGGCCAG TCCTTGAGGA GACCATTCAC 
AAGCCAGTCT CCTCTAATTC TCCCAAACCA GTTCCCGAGG AAGTGAAAGC GACCTATTGG 
CCTCGACCCC AGTTGGTCAC AGGATACTGT ACATATCTTC CCTTTCGAGG ATTTGACATT 
ACCCAGACAC CACCAGCCTT CATCAGTGTG TTACCGACTT TGCCCCAGTA TAGCATTTAC 
ACCCCCCTGG CCAGCGTTCC TACTGAGTAT CACCTGCAAA GACCAATACC AGTGGTGCCG 
TCTTTTGTAG CCAGTGACAT AACGGAAGTG CATTTTGAGG CTCATCATTT CAGTGCTGAG 
AATGCCCATG GTAACCAGAT TGCCTCCGAA ACACAGATCC TTGAGGAATC TTTGGAAACA 
CCAGTGAAGT CAGAGTGCAG CTCTGATGGT GCTGATATAG CCCAGAGTGA GGCTAGCGGA 
AACGAGGACT ACTGTGGAGA TTCTGCCAAG TGGGAAGTAA ATCCAGAAAG CACCAATAAA 
GTAACAAATA TTCCATGTAC ACAAGTGGTT GCTGTACAGG TGTCTTGGAA TATAACACAT 
CAGGAAGTCA ATACTGAGCC ATATGGTCCT TTTGAGGCAC AACAGGGAGG TATTTCACAG 
ATTGAAAAGG AGTACCAGGT TTTACAAGAG CAGCTTAAGG AAGCATGTGA AAATTATGAA 
CACAGCAATG TCAGGGGCTC AGAAGAAACC AAGGACTTGG AAGAAAAGTT GAAAAGGAAC 
GTTGAAGAAA ATAAGATCTC AAAGACAGAA CTAGATTGGT TCCTTGAAGA TTTGAACAAG 
GAAATTAAAA AATGGGAACA GGAGAAAAAA GAAATCCAAG AAAGACTAAA GGCACTGAAG 
AAGAAAATGA AAAAGGTGTT GAATGCCAGC AGAATGTATA CACAGGAAAA CGATGGAAAG 
GAAAAGGCAC TTGAATTACT TCTGGATCAG TCCCTTGAAA TCAGTAATAC ATTTACAAAT 
GAGAAAATGA AAATAGAAGA GAGTATAAAG AAAGGGAAAG AGCATTATGA AGAGAGTCTT 
CAGAGGGCTC TGGATGCAGA GGTATCTGTG CTTGAAAACT GGAAGGATGT GGAGGTGTGT 
AAGTTACAGA GCATGGAGTC ACAGGCCGAA GGGCTTCTCA AGAACTTGAA GCTAATGAGC 
AGTGATTCGG CCGCATATTC TGACATGGAC TCAGACATAC ATTCATGGGA ATCGTTTCTT 
TCTGATGTTA GAGAAGAAAT TGAGAAAGCA AAGTCTCAGT TTGAAGAACA AATTCAGGCA 
ATTAAAAATG GTTCTCGGCT CAGTGAACTT CCTAAAGTGC AGATTTCTGA GCTTTCGTTT 
CCTACCTGTA ACACAATTCA TCCTAAATTA CTTCCTGAGT CTTCAGGCCA GGAGGGTCAA 
GGTCCCTCCA CTTCTGCAAG TCATGGGACC GGAGACCAGA CAGCAGCTCC CGAGGAGTCA 
AGTCTGGGTT CTGCTGCTGA CAGCCCAGTG GAGTCGCCCT CCTGCAGCCA GAAAGCCCCC 
CAGCTGCCAG AGCGTGAGGG CACTGGCCAG GCAGCTGGGC CAGAACTAGG CCTGATGGCT 
GATCAGAAGT TACCTGTTGC TCCAGAGCGT GCCACTCGTC CAAGCCAGTC TCCAAAGAAG 
CCGTTCAATA GCATTATTGA GCACCTGTCG GTGGTGTTCC CTTGTTACAG CAGCACGGAG 
CTTGCTGGTT TTATTAAAAA AGTTAGAAAC AAAAACAAGA CCTCTCTGTC TGGATTGAGT 
ATTGACGAAA TTGTGCAAAG AGTGACACAA CACATCCTTG ATGAAGAGAG AAAGAAAAAG 
CCAAATCCAG GAAAGGACCG GAGGCCGTCT GCACCCAGCT CTGCTGTTCC TGCACCCAGG 
GCCGCCCAGG ACCCGCCCTC CGTGCCTGTC AGAGCATCAC CCGAAACCAG GGGGCCGAAG 
ACCGAAGACG TCCCTGTGTC CTGTGCCAAT TCCTGTGAAA TGTGCCAAGA AGCATTCAAG 
TCCAAACACG TGCGTGAGCT GCAGTGTGGG CACAGGCTTC ACAAAGGGTG CTTCAAGCAG 
TGGTTGAAGA GCCAGCGCAC CTGCCCAGCC TGCCTGGGTC ACGACCTCCT GTCAGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986006.1
CDS	105..5267
Translation	MKKHVRGWDPQNKAANALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130616.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130616.2

ORF Insert Sequence:

ATGAAAAAAC ACGTTAGAGG CTGGGATCCT CAGAATAAAG CTGCAAATGC ACTTGGTGAT 
GGAAAGAGAG CTACCATTCT GAAGAACAAT TGGACAAAGG GTCATTATCG TTATTGTGCT 
GCTCTTTCTA TGCTGGGGGA ATATGACTGG GCCCTGCAAG CAAACATAAA AGCTCAAAAA 
CTCTGTAAAA ATGACCCTGA GGGAATCAAG GATCTAATTC AGCAGCATGT AAAGTTACAA 
AAACAGATAG AAGACCTGCA AGGTCGAACA CCAGCTAGTA ATCCAGTCAA AGCCTTCTGT 
GAAAGCAGGG CCTACATACC TAGTTTATCA GCACCTGCAT TTAGTACTTC ACTTAACTTT 
GTGGAAACTG AAAAAAATTT CAGAAAAACT AACCACGAAA TGGCAAACGG TGGTAATCAG 
AATCCAAAGG TGGTAGATGA AGCTCTGAAA GTAGATGATT GTGACTGTCA TCCTGAATTT 
GTACCACCAT CAAGTCAGCC TCCAAAATAT AAAGGGAAAC AAAAAGCTCG AAACAATGAA 
TTGGAGAAGT TCAGCCCTGG TTCACAAGAA ACTTTACCAG TAGATTTGAA AAACATCTTG 
GAGAAACAGT TTTCTAAATC TTCCAGAGCT GCACACCAGG ATTTTGCTAA TATAATGAAA 
ATGCTGAGAA GCTTAATCCA GGATGGCTAC ACGGCCTTGC TGGAGCAGCG CTGCCGCAGT 
GCTGCCCAGG CCTTCACAGA GTTGCTCAAC GGTTTAGATC CTCAAAAAAT AAAGCAATTG 
AATCTGGCCA TGATTAACTA CGTGTTAGTA GTCTACGGAC TTGCCATTTC ACTCCTTGGA 
ATAGGACAGC CTGAGGAGTT ATCTGAAGCT GAAAACCAAT TTAAGAGGAT GATTGAACAC 
TACCCCAATG AGGGACTTGA TTGCTTGGCC TACTGTGGAA TTGGAAAAGT ATATTTGAAA 
AAAAACAGAT TTTTAGAAGC TCTTAATCAT TTTGAGAAAG CAAGAACCTT GATCTGTCGT 
CTTCCTGGAG TGTTAACTTG GCCCACGAGT AATGTGATTA TTGAGGAGAC TCAGCCAGGG 
AAGATAAAGA TGCTGTTAGA AAAATTCGTT GAAGAATGCA GGTTCCCTCC AGTGCCGGAT 
GCCACTTGTT GCTATCAGAA GTGCCATGGA TATTCCAAAA TTCAGATATA TATAACCGAT 
CCAGACTTCA AGGGTTTTAT ACGAATCAGC TGTTGCCAGT ATTGTAAAAT AGAATTTCAC 
ATGAACTGCT GGAAGAAGTT AAAAACAACA ACCTTCAATG ATAAAATTGA CAAGGATTTT 
CTGCAAGGAA TTTGTCTTAC TCCTGATTGT AAAGGAATCA TTTCAAAGAT TATCATCTTC 
AGCAGTGGTG GTCAAGTTAA ATGTGAATTT GAACACAAGG TCATTAAAGA AAAGCTTCCT 
CCAAGACCTA TTCTGAAACA GAAATGTTCT AGCCTAGAGA AGTTGAGGCT GAAAGAAGAC 
AAAAAATTGA AACGAAAGCT CCAAAAAAAG GAAGCAAAAA AATTAGCACA AGAAAGACTG 
GAAGAGGACC TAAGGGAAAG TAACCCACCA AAAACTGAAG AGCAGAGAGA AACTATCAGC 
CGTGTCCAAA GTTGCCAGTT CCTTGATGAC AGAATTCTGC AGTGCATAAA GCAGTACGCT 
GACAAGATTA AGTCCGGGAT ACTGAATACT TCCAAGCTTC TCAAAGAATT GCTTTCTTGG 
AAGGTTTTAA CCACAGAAGA CTACACAACG TGTTTTTCTA GCAGAAATTT CCTGAATGAA 
GCAGTGGACT ATGTTATTCG TCATTTGATC CAGGAAAAGA ACAGAGTAAA GACAAGGGTA 
TTTCTGCATG TTTTGAGTGA GCTTAAAGAA GTGGAGCCAA AACTAGCCAC CTGGATCCAG 
AAACTTAATA GCTTTGGCTT AGATGCCACA GGACCTTTCT TTTCTCGTTA TGGGGCTCCT 
CTTAAGGAGC TTGATTTTAG CATCATGACT TTTCTCTGGA ATGAGAAATA TGGTCATAAG 
CTAGGCTCTA TAGAAGGAAA GCAACTTGAT TATTTCTATG AGCCTACGTC ACTGAAGGAA 
GCGCGGTGTT TAATATGGCT GCTGGAAGAA CACAGAGACA AGTTCCCGGC ACTGCATAAT 
GCCCTGGACG AGTTCTTTGA TATAATGGAC AGCCGCTGCA CTGTGTTAAG GAAACAGGAC 
AGTGGTGACG TGCCATGTAA TTTTACCAAG ATAAAAAACA AAGGCAAGAA AAAGAAGCCA 
AAAGATTCAA AGCCTATGTT AGTTGGATCT GGAACACCTT CAGTCACACC AAACAATGAG 
ACTTTCACTT CAGGTGAAGA CCATATCAAA CGCAGTTTAA ATTCTGCGGG CCCCTTTGTA 
GTGCCTGATC ACCTTCGGCA GGATGTAGAA GAATTTGAAG CTCTGTATGA ACAGCATAGT 
AATGAGTATG TTGTCCGTAA TAAGAAGCTG TGGGACATAA ACCCAAAACA AAAATGTTCA 
ACTCTATATG ATTATTTCTC TCAGTTGTTG GAGGAACATG GTCCCTTGGA CATGAGTAAC 
AAGATGTTTT CTGAAGAATA TGAGTTCTTC CCAGAAGAAA CTCGACAGAT ACTAGAAAAA 
GCAGGAGGTT TGAAAGCGTT TCTCCTCGGA TGTCCTCGTT TTGTTGTGAT TGACAACTGT 
ATTGCATTGA AGAAAGTTGC ATTACGGCTC AAGAAAAAGA GAAAGAAGAA AAACATTAAG 
ACAAAAGTAG AAGAAATTTC AAAAACAGGA GAGTATTTAC GAGTTAAACT ACCACTGAAT 
CCAACTGCTA GGGAATTTAA ACCAGATGTA AAGTCCAAAC CAGTGTCTGA CTTACCTTTA 
GCACCAGCTT CTGAGGACGT GAAGCCTCAG CATGCATCTG CTAATTCTCC CCAATCAGCT 
CGTGACACTG GGAAGCCAAA ACCAGTTTCC GATAATTCTT CCAGATCAGT TTCTGAGGAT 
GAGAAGCCCA AAGGGATATC TTCTGATTCT CCCAGGCCAG TCCTTGAGGA GACCATTCAC 
AAGCCAGTCT CCTCTAATTC TCCCAAACCA GTTCCCGAGG AAGTGAAAGC GACCTATTGG 
CCTCGACCCC AGTTGGTCAC AGGATACTGT ACATATCTTC CCTTTCGAGG ATTTGACATT 
ACCCAGACAC CACCAGCCTT CATCAGTGTG TTACCGACTT TGCCCCAGTA TAGCATTTAC 
ACCCCCCTGG CCAGCGTTCC TACTGAGTAT CACCTGCAAA GACCAATACC AGTGGTGCCG 
TCTTTTGTAG CCAGTGACAT AACGGAAGTG CATTTTGAGG CTCATCATTT CAGTGCTGAG 
AATGCCCATG GTAACCAGAT TGCCTCCGAA ACACAGATCC TTGAGGAATC TTTGGAAACA 
CCAGTGAAGT CAGAGTGCAG CTCTGATGGT GCTGATATAG CCCAGAGTGA GGCTAGCGGA 
AACGAGGACT ACTGTGGAGA TTCTGCCAAG TGGGAAGTAA ATCCAGAAAG CACCAATAAA 
GTAACAAATA TTCCATGTAC ACAAGTGGTT GCTGTACAGG TGTCTTGGAA TATAACACAT 
CAGGAAGTCA ATACTGAGCC ATATGGTCCT TTTGAGGCAC AACAGGGAGG TATTTCACAG 
ATTGAAAAGG AGTACCAGGT TTTACAAGAG CAGCTTAAGG AAGCATGTGA AAATTATGAA 
CACAGCAATG TCAGGGGCTC AGAAGAAACC AAGGACTTGG AAGAAAAGTT GAAAAGGAAC 
GTTGAAGAAA ATAAGATCTC AAAGACAGAA CTAGATTGGT TCCTTGAAGA TTTGAACAAG 
GAAATTAAAA AATGGGAACA GGAGAAAAAA GAAATCCAAG AAAGACTAAA GGCACTGAAG 
AAGAAAATGA AAAAGGTGTT GAATGCCAGC AGAATGTATA CACAGGAAAA CGATGGAAAG 
GAAAAGGCAC TTGAATTACT TCTGGATCAG TCCCTTGAAA TCAGTAATAC ATTTACAAAT 
GAGAAAATGA AAATAGAAGA GAGTATAAAG AAAGGGAAAG AGCATTATGA AGAGAGTCTT 
CAGAGGGCTC TGGATGCAGA GGTATCTGTG CTTGAAAACT GGAAGGATGT GGAGGTGTGT 
AAGTTACAGA GCATGGAGTC ACAGGCCGAA GGGCTTCTCA AGAACTTGAA GCTAATGAGC 
AGTGATTCGG CCGCATATTC TGACATGGAC TCAGACATAC ATTCATGGGA ATCGTTTCTT 
TCTGATGTTA GAGAAGAAAT TGAGAAAGCA AAGTCTCAGT TTGAAGAACA AATTCAGGCA 
ATTAAAAATG GTTCTCGGCT CAGTGAACTT CCTAAAGTGC AGATTTCTGA GCTTTCGTTT 
CCTACCTGTA ACACAATTCA TCCTAAATTA CTTCCTGAGT CTTCAGGCCA GGAGGGTCAA 
GGTCCCTCCA CTTCTGCAAG TCATGGGACC GGAGACCAGA CAGCAGCTCC CGAGGAGTCA 
AGTCTGGGTT CTGCTGCTGA CAGCCCAGTG GAGTCGCCCT CCTGCAGCCA GAAAGCCCCC 
CAGCTGCCAG AGCGTGAGGG CACTGGCCAG GCAGCTGGGC CAGAACTAGG CCTGATGGCT 
GATCAGAAGT TACCTGTTGC TCCAGAGCGT GCCACTCGTC CAAGCCAGTC TCCAAAGAAG 
CCGTTCAATA GCATTATTGA GCACCTGTCG GTGGTGTTCC CTTGTTACAG CAGCACGGAG 
CTTGCTGGTT TTATTAAAAA AGTTAGAAAC AAAAACAAGA CCTCTCTGTC TGGATTGAGT 
ATTGACGAAA TTGTGCAAAG AGTGACACAA CACATCCTTG ATGAAGAGAG AAAGAAAAAG 
CCAAATCCAG GAAAGGACCG GAGGCCGTCT GCACCCAGCT CTGCTGTTCC TGCACCCAGG 
GCCGCCCAGG ACCCGCCCTC CGTGCCTGTC AGAGCATCAC CCGAAACCAG GGGGCCGAAG 
ACCGAAGACG TCCCTGTGTC CTGTGCCAAT TCCTGTGAAA TGTGCCAAGA AGCATTCAAG 
TCCAAACACG TGCGTGAGCT GCAGTGTGGG CACAGGCTTC ACAAAGGGTG CTTCAAGCAG 
TGGTTGAAGA GCCAGCGCAC CTGCCCAGCC TGCCTGGGTC ACGACCTCCT GTCAGAGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130617.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986007.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

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CloneID	OOv13964
Clone ID Related Accession (Same CDS sequence)	XM_012130617.2
Accession Version	XM_012130617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCGTC TGGAGGTGGT TGTTTGTATG AGAAATGCAC TTGGTGATGG AAAGAGAGCT 
ACCATTCTGA AGAACAATTG GACAAAGGGT CATTATCGTT ATTGTGCTGC TCTTTCTATG 
CTGGGGGAAT ATGACTGGGC CCTGCAAGCA AACATAAAAG CTCAAAAACT CTGTAAAAAT 
GACCCTGAGG GAATCAAGGA TCTAATTCAG CAGCATGTAA AGTTACAAAA ACAGATAGAA 
GACCTGCAAG GTCGAACACC AGCTAGTAAT CCAGTCAAAG CCTTCTGTGA AAGCAGGGCC 
TACATACCTA GTTTATCAGC ACCTGCATTT AGTACTTCAC TTAACTTTGT GGAAACTGAA 
AAAAATTTCA GAAAAACTAA CCACGAAATG GCAAACGGTG GTAATCAGAA TCCAAAGGTG 
GTAGATGAAG CTCTGAAAGT AGATGATTGT GACTGTCATC CTGAATTTGT ACCACCATCA 
AGTCAGCCTC CAAAATATAA AGGGAAACAA AAAGCTCGAA ACAATGAATT GGAGAAGTTC 
AGCCCTGGTT CACAAGAAAC TTTACCAGTA GATTTGAAAA ACATCTTGGA GAAACAGTTT 
TCTAAATCTT CCAGAGCTGC ACACCAGGAT TTTGCTAATA TAATGAAAAT GCTGAGAAGC 
TTAATCCAGG ATGGCTACAC GGCCTTGCTG GAGCAGCGCT GCCGCAGTGC TGCCCAGGCC 
TTCACAGAGT TGCTCAACGG TTTAGATCCT CAAAAAATAA AGCAATTGAA TCTGGCCATG 
ATTAACTACG TGTTAGTAGT CTACGGACTT GCCATTTCAC TCCTTGGAAT AGGACAGCCT 
GAGGAGTTAT CTGAAGCTGA AAACCAATTT AAGAGGATGA TTGAACACTA CCCCAATGAG 
GGACTTGATT GCTTGGCCTA CTGTGGAATT GGAAAAGTAT ATTTGAAAAA AAACAGATTT 
TTAGAAGCTC TTAATCATTT TGAGAAAGCA AGAACCTTGA TCTGTCGTCT TCCTGGAGTG 
TTAACTTGGC CCACGAGTAA TGTGATTATT GAGGAGACTC AGCCAGGGAA GATAAAGATG 
CTGTTAGAAA AATTCGTTGA AGAATGCAGG TTCCCTCCAG TGCCGGATGC CACTTGTTGC 
TATCAGAAGT GCCATGGATA TTCCAAAATT CAGATATATA TAACCGATCC AGACTTCAAG 
GGTTTTATAC GAATCAGCTG TTGCCAGTAT TGTAAAATAG AATTTCACAT GAACTGCTGG 
AAGAAGTTAA AAACAACAAC CTTCAATGAT AAAATTGACA AGGATTTTCT GCAAGGAATT 
TGTCTTACTC CTGATTGTAA AGGAATCATT TCAAAGATTA TCATCTTCAG CAGTGGTGGT 
CAAGTTAAAT GTGAATTTGA ACACAAGGTC ATTAAAGAAA AGCTTCCTCC AAGACCTATT 
CTGAAACAGA AATGTTCTAG CCTAGAGAAG TTGAGGCTGA AAGAAGACAA AAAATTGAAA 
CGAAAGCTCC AAAAAAAGGA AGCAAAAAAA TTAGCACAAG AAAGACTGGA AGAGGACCTA 
AGGGAAAGTA ACCCACCAAA AACTGAAGAG CAGAGAGAAA CTATCAGCCG TGTCCAAAGT 
TGCCAGTTCC TTGATGACAG AATTCTGCAG TGCATAAAGC AGTACGCTGA CAAGATTAAG 
TCCGGGATAC TGAATACTTC CAAGCTTCTC AAAGAATTGC TTTCTTGGAA GGTTTTAACC 
ACAGAAGACT ACACAACGTG TTTTTCTAGC AGAAATTTCC TGAATGAAGC AGTGGACTAT 
GTTATTCGTC ATTTGATCCA GGAAAAGAAC AGAGTAAAGA CAAGGGTATT TCTGCATGTT 
TTGAGTGAGC TTAAAGAAGT GGAGCCAAAA CTAGCCACCT GGATCCAGAA ACTTAATAGC 
TTTGGCTTAG ATGCCACAGG ACCTTTCTTT TCTCGTTATG GGGCTCCTCT TAAGGAGCTT 
GATTTTAGCA TCATGACTTT TCTCTGGAAT GAGAAATATG GTCATAAGCT AGGCTCTATA 
GAAGGAAAGC AACTTGATTA TTTCTATGAG CCTACGTCAC TGAAGGAAGC GCGGTGTTTA 
ATATGGCTGC TGGAAGAACA CAGAGACAAG TTCCCGGCAC TGCATAATGC CCTGGACGAG 
TTCTTTGATA TAATGGACAG CCGCTGCACT GTGTTAAGGA AACAGGACAG TGGTGACGTG 
CCATGTAATT TTACCAAGAT AAAAAACAAA GGCAAGAAAA AGAAGCCAAA AGATTCAAAG 
CCTATGTTAG TTGGATCTGG AACACCTTCA GTCACACCAA ACAATGAGAC TTTCACTTCA 
GGTGAAGACC ATATCAAACG CAGTTTAAAT TCTGCGGGCC CCTTTGTAGT GCCTGATCAC 
CTTCGGCAGG ATGTAGAAGA ATTTGAAGCT CTGTATGAAC AGCATAGTAA TGAGTATGTT 
GTCCGTAATA AGAAGCTGTG GGACATAAAC CCAAAACAAA AATGTTCAAC TCTATATGAT 
TATTTCTCTC AGTTGTTGGA GGAACATGGT CCCTTGGACA TGAGTAACAA GATGTTTTCT 
GAAGAATATG AGTTCTTCCC AGAAGAAACT CGACAGATAC TAGAAAAAGC AGGAGGTTTG 
AAAGCGTTTC TCCTCGGATG TCCTCGTTTT GTTGTGATTG ACAACTGTAT TGCATTGAAG 
AAAGTTGCAT TACGGCTCAA GAAAAAGAGA AAGAAGAAAA ACATTAAGAC AAAAGTAGAA 
GAAATTTCAA AAACAGGAGA GTATTTACGA GTTAAACTAC CACTGAATCC AACTGCTAGG 
GAATTTAAAC CAGATGTAAA GTCCAAACCA GTGTCTGACT TACCTTTAGC ACCAGCTTCT 
GAGGACGTGA AGCCTCAGCA TGCATCTGCT AATTCTCCCC AATCAGCTCG TGACACTGGG 
AAGCCAAAAC CAGTTTCCGA TAATTCTTCC AGATCAGTTT CTGAGGATGA GAAGCCCAAA 
GGGATATCTT CTGATTCTCC CAGGCCAGTC CTTGAGGAGA CCATTCACAA GCCAGTCTCC 
TCTAATTCTC CCAAACCAGT TCCCGAGGAA GTGAAAGCGA CCTATTGGCC TCGACCCCAG 
TTGGTCACAG GATACTGTAC ATATCTTCCC TTTCGAGGAT TTGACATTAC CCAGACACCA 
CCAGCCTTCA TCAGTGTGTT ACCGACTTTG CCCCAGTATA GCATTTACAC CCCCCTGGCC 
AGCGTTCCTA CTGAGTATCA CCTGCAAAGA CCAATACCAG TGGTGCCGTC TTTTGTAGCC 
AGTGACATAA CGGAAGTGCA TTTTGAGGCT CATCATTTCA GTGCTGAGAA TGCCCATGGT 
AACCAGATTG CCTCCGAAAC ACAGATCCTT GAGGAATCTT TGGAAACACC AGTGAAGTCA 
GAGTGCAGCT CTGATGGTGC TGATATAGCC CAGAGTGAGG CTAGCGGAAA CGAGGACTAC 
TGTGGAGATT CTGCCAAGTG GGAAGTAAAT CCAGAAAGCA CCAATAAAGT AACAAATATT 
CCATGTACAC AAGTGGTTGC TGTACAGGTG TCTTGGAATA TAACACATCA GGAAGTCAAT 
ACTGAGCCAT ATGGTCCTTT TGAGGCACAA CAGGGAGGTA TTTCACAGAT TGAAAAGGAG 
TACCAGGTTT TACAAGAGCA GCTTAAGGAA GCATGTGAAA ATTATGAACA CAGCAATGTC 
AGGGGCTCAG AAGAAACCAA GGACTTGGAA GAAAAGTTGA AAAGGAACGT TGAAGAAAAT 
AAGATCTCAA AGACAGAACT AGATTGGTTC CTTGAAGATT TGAACAAGGA AATTAAAAAA 
TGGGAACAGG AGAAAAAAGA AATCCAAGAA AGACTAAAGG CACTGAAGAA GAAAATGAAA 
AAGGTGTTGA ATGCCAGCAG AATGTATACA CAGGAAAACG ATGGAAAGGA AAAGGCACTT 
GAATTACTTC TGGATCAGTC CCTTGAAATC AGTAATACAT TTACAAATGA GAAAATGAAA 
ATAGAAGAGA GTATAAAGAA AGGGAAAGAG CATTATGAAG AGAGTCTTCA GAGGGCTCTG 
GATGCAGAGG TATCTGTGCT TGAAAACTGG AAGGATGTGG AGGTGTGTAA GTTACAGAGC 
ATGGAGTCAC AGGCCGAAGG GCTTCTCAAG AACTTGAAGC TAATGAGCAG TGATTCGGCC 
GCATATTCTG ACATGGACTC AGACATACAT TCATGGGAAT CGTTTCTTTC TGATGTTAGA 
GAAGAAATTG AGAAAGCAAA GTCTCAGTTT GAAGAACAAA TTCAGGCAAT TAAAAATGGT 
TCTCGGCTCA GTGAACTTCC TAAAGTGCAG ATTTCTGAGC TTTCGTTTCC TACCTGTAAC 
ACAATTCATC CTAAATTACT TCCTGAGTCT TCAGGCCAGG AGGGTCAAGG TCCCTCCACT 
TCTGCAAGTC ATGGGACCGG AGACCAGACA GCAGCTCCCG AGGAGTCAAG TCTGGGTTCT 
GCTGCTGACA GCCCAGTGGA GTCGCCCTCC TGCAGCCAGA AAGCCCCCCA GCTGCCAGAG 
CGTGAGGGCA CTGGCCAGGC AGCTGGGCCA GAACTAGGCC TGATGGCTGA TCAGAAGTTA 
CCTGTTGCTC CAGAGCGTGC CACTCGTCCA AGCCAGTCTC CAAAGAAGCC GTTCAATAGC 
ATTATTGAGC ACCTGTCGGT GGTGTTCCCT TGTTACAGCA GCACGGAGCT TGCTGGTTTT 
ATTAAAAAAG TTAGAAACAA AAACAAGACC TCTCTGTCTG GATTGAGTAT TGACGAAATT 
GTGCAAAGAG TGACACAACA CATCCTTGAT GAAGAGAGAA AGAAAAAGCC AAATCCAGGA 
AAGGACCGGA GGCCGTCTGC ACCCAGCTCT GCTGTTCCTG CACCCAGGGC CGCCCAGGAC 
CCGCCCTCCG TGCCTGTCAG AGCATCACCC GAAACCAGGG GGCCGAAGAC CGAAGACGTC 
CCTGTGTCCT GTGCCAATTC CTGTGAAATG TGCCAAGAAG CATTCAAGTC CAAACACGTG 
CGTGAGCTGC AGTGTGGGCA CAGGCTTCAC AAAGGGTGCT TCAAGCAGTG GTTGAAGAGC 
CAGCGCACCT GCCCAGCCTG CCTGGGTCAC GACCTCCTGT CAGAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986007.1
CDS	7468..12618
Translation	MIRLEVVVCMRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130617.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130617.2

ORF Insert Sequence:

ATGATCCGTC TGGAGGTGGT TGTTTGTATG AGAAATGCAC TTGGTGATGG AAAGAGAGCT 
ACCATTCTGA AGAACAATTG GACAAAGGGT CATTATCGTT ATTGTGCTGC TCTTTCTATG 
CTGGGGGAAT ATGACTGGGC CCTGCAAGCA AACATAAAAG CTCAAAAACT CTGTAAAAAT 
GACCCTGAGG GAATCAAGGA TCTAATTCAG CAGCATGTAA AGTTACAAAA ACAGATAGAA 
GACCTGCAAG GTCGAACACC AGCTAGTAAT CCAGTCAAAG CCTTCTGTGA AAGCAGGGCC 
TACATACCTA GTTTATCAGC ACCTGCATTT AGTACTTCAC TTAACTTTGT GGAAACTGAA 
AAAAATTTCA GAAAAACTAA CCACGAAATG GCAAACGGTG GTAATCAGAA TCCAAAGGTG 
GTAGATGAAG CTCTGAAAGT AGATGATTGT GACTGTCATC CTGAATTTGT ACCACCATCA 
AGTCAGCCTC CAAAATATAA AGGGAAACAA AAAGCTCGAA ACAATGAATT GGAGAAGTTC 
AGCCCTGGTT CACAAGAAAC TTTACCAGTA GATTTGAAAA ACATCTTGGA GAAACAGTTT 
TCTAAATCTT CCAGAGCTGC ACACCAGGAT TTTGCTAATA TAATGAAAAT GCTGAGAAGC 
TTAATCCAGG ATGGCTACAC GGCCTTGCTG GAGCAGCGCT GCCGCAGTGC TGCCCAGGCC 
TTCACAGAGT TGCTCAACGG TTTAGATCCT CAAAAAATAA AGCAATTGAA TCTGGCCATG 
ATTAACTACG TGTTAGTAGT CTACGGACTT GCCATTTCAC TCCTTGGAAT AGGACAGCCT 
GAGGAGTTAT CTGAAGCTGA AAACCAATTT AAGAGGATGA TTGAACACTA CCCCAATGAG 
GGACTTGATT GCTTGGCCTA CTGTGGAATT GGAAAAGTAT ATTTGAAAAA AAACAGATTT 
TTAGAAGCTC TTAATCATTT TGAGAAAGCA AGAACCTTGA TCTGTCGTCT TCCTGGAGTG 
TTAACTTGGC CCACGAGTAA TGTGATTATT GAGGAGACTC AGCCAGGGAA GATAAAGATG 
CTGTTAGAAA AATTCGTTGA AGAATGCAGG TTCCCTCCAG TGCCGGATGC CACTTGTTGC 
TATCAGAAGT GCCATGGATA TTCCAAAATT CAGATATATA TAACCGATCC AGACTTCAAG 
GGTTTTATAC GAATCAGCTG TTGCCAGTAT TGTAAAATAG AATTTCACAT GAACTGCTGG 
AAGAAGTTAA AAACAACAAC CTTCAATGAT AAAATTGACA AGGATTTTCT GCAAGGAATT 
TGTCTTACTC CTGATTGTAA AGGAATCATT TCAAAGATTA TCATCTTCAG CAGTGGTGGT 
CAAGTTAAAT GTGAATTTGA ACACAAGGTC ATTAAAGAAA AGCTTCCTCC AAGACCTATT 
CTGAAACAGA AATGTTCTAG CCTAGAGAAG TTGAGGCTGA AAGAAGACAA AAAATTGAAA 
CGAAAGCTCC AAAAAAAGGA AGCAAAAAAA TTAGCACAAG AAAGACTGGA AGAGGACCTA 
AGGGAAAGTA ACCCACCAAA AACTGAAGAG CAGAGAGAAA CTATCAGCCG TGTCCAAAGT 
TGCCAGTTCC TTGATGACAG AATTCTGCAG TGCATAAAGC AGTACGCTGA CAAGATTAAG 
TCCGGGATAC TGAATACTTC CAAGCTTCTC AAAGAATTGC TTTCTTGGAA GGTTTTAACC 
ACAGAAGACT ACACAACGTG TTTTTCTAGC AGAAATTTCC TGAATGAAGC AGTGGACTAT 
GTTATTCGTC ATTTGATCCA GGAAAAGAAC AGAGTAAAGA CAAGGGTATT TCTGCATGTT 
TTGAGTGAGC TTAAAGAAGT GGAGCCAAAA CTAGCCACCT GGATCCAGAA ACTTAATAGC 
TTTGGCTTAG ATGCCACAGG ACCTTTCTTT TCTCGTTATG GGGCTCCTCT TAAGGAGCTT 
GATTTTAGCA TCATGACTTT TCTCTGGAAT GAGAAATATG GTCATAAGCT AGGCTCTATA 
GAAGGAAAGC AACTTGATTA TTTCTATGAG CCTACGTCAC TGAAGGAAGC GCGGTGTTTA 
ATATGGCTGC TGGAAGAACA CAGAGACAAG TTCCCGGCAC TGCATAATGC CCTGGACGAG 
TTCTTTGATA TAATGGACAG CCGCTGCACT GTGTTAAGGA AACAGGACAG TGGTGACGTG 
CCATGTAATT TTACCAAGAT AAAAAACAAA GGCAAGAAAA AGAAGCCAAA AGATTCAAAG 
CCTATGTTAG TTGGATCTGG AACACCTTCA GTCACACCAA ACAATGAGAC TTTCACTTCA 
GGTGAAGACC ATATCAAACG CAGTTTAAAT TCTGCGGGCC CCTTTGTAGT GCCTGATCAC 
CTTCGGCAGG ATGTAGAAGA ATTTGAAGCT CTGTATGAAC AGCATAGTAA TGAGTATGTT 
GTCCGTAATA AGAAGCTGTG GGACATAAAC CCAAAACAAA AATGTTCAAC TCTATATGAT 
TATTTCTCTC AGTTGTTGGA GGAACATGGT CCCTTGGACA TGAGTAACAA GATGTTTTCT 
GAAGAATATG AGTTCTTCCC AGAAGAAACT CGACAGATAC TAGAAAAAGC AGGAGGTTTG 
AAAGCGTTTC TCCTCGGATG TCCTCGTTTT GTTGTGATTG ACAACTGTAT TGCATTGAAG 
AAAGTTGCAT TACGGCTCAA GAAAAAGAGA AAGAAGAAAA ACATTAAGAC AAAAGTAGAA 
GAAATTTCAA AAACAGGAGA GTATTTACGA GTTAAACTAC CACTGAATCC AACTGCTAGG 
GAATTTAAAC CAGATGTAAA GTCCAAACCA GTGTCTGACT TACCTTTAGC ACCAGCTTCT 
GAGGACGTGA AGCCTCAGCA TGCATCTGCT AATTCTCCCC AATCAGCTCG TGACACTGGG 
AAGCCAAAAC CAGTTTCCGA TAATTCTTCC AGATCAGTTT CTGAGGATGA GAAGCCCAAA 
GGGATATCTT CTGATTCTCC CAGGCCAGTC CTTGAGGAGA CCATTCACAA GCCAGTCTCC 
TCTAATTCTC CCAAACCAGT TCCCGAGGAA GTGAAAGCGA CCTATTGGCC TCGACCCCAG 
TTGGTCACAG GATACTGTAC ATATCTTCCC TTTCGAGGAT TTGACATTAC CCAGACACCA 
CCAGCCTTCA TCAGTGTGTT ACCGACTTTG CCCCAGTATA GCATTTACAC CCCCCTGGCC 
AGCGTTCCTA CTGAGTATCA CCTGCAAAGA CCAATACCAG TGGTGCCGTC TTTTGTAGCC 
AGTGACATAA CGGAAGTGCA TTTTGAGGCT CATCATTTCA GTGCTGAGAA TGCCCATGGT 
AACCAGATTG CCTCCGAAAC ACAGATCCTT GAGGAATCTT TGGAAACACC AGTGAAGTCA 
GAGTGCAGCT CTGATGGTGC TGATATAGCC CAGAGTGAGG CTAGCGGAAA CGAGGACTAC 
TGTGGAGATT CTGCCAAGTG GGAAGTAAAT CCAGAAAGCA CCAATAAAGT AACAAATATT 
CCATGTACAC AAGTGGTTGC TGTACAGGTG TCTTGGAATA TAACACATCA GGAAGTCAAT 
ACTGAGCCAT ATGGTCCTTT TGAGGCACAA CAGGGAGGTA TTTCACAGAT TGAAAAGGAG 
TACCAGGTTT TACAAGAGCA GCTTAAGGAA GCATGTGAAA ATTATGAACA CAGCAATGTC 
AGGGGCTCAG AAGAAACCAA GGACTTGGAA GAAAAGTTGA AAAGGAACGT TGAAGAAAAT 
AAGATCTCAA AGACAGAACT AGATTGGTTC CTTGAAGATT TGAACAAGGA AATTAAAAAA 
TGGGAACAGG AGAAAAAAGA AATCCAAGAA AGACTAAAGG CACTGAAGAA GAAAATGAAA 
AAGGTGTTGA ATGCCAGCAG AATGTATACA CAGGAAAACG ATGGAAAGGA AAAGGCACTT 
GAATTACTTC TGGATCAGTC CCTTGAAATC AGTAATACAT TTACAAATGA GAAAATGAAA 
ATAGAAGAGA GTATAAAGAA AGGGAAAGAG CATTATGAAG AGAGTCTTCA GAGGGCTCTG 
GATGCAGAGG TATCTGTGCT TGAAAACTGG AAGGATGTGG AGGTGTGTAA GTTACAGAGC 
ATGGAGTCAC AGGCCGAAGG GCTTCTCAAG AACTTGAAGC TAATGAGCAG TGATTCGGCC 
GCATATTCTG ACATGGACTC AGACATACAT TCATGGGAAT CGTTTCTTTC TGATGTTAGA 
GAAGAAATTG AGAAAGCAAA GTCTCAGTTT GAAGAACAAA TTCAGGCAAT TAAAAATGGT 
TCTCGGCTCA GTGAACTTCC TAAAGTGCAG ATTTCTGAGC TTTCGTTTCC TACCTGTAAC 
ACAATTCATC CTAAATTACT TCCTGAGTCT TCAGGCCAGG AGGGTCAAGG TCCCTCCACT 
TCTGCAAGTC ATGGGACCGG AGACCAGACA GCAGCTCCCG AGGAGTCAAG TCTGGGTTCT 
GCTGCTGACA GCCCAGTGGA GTCGCCCTCC TGCAGCCAGA AAGCCCCCCA GCTGCCAGAG 
CGTGAGGGCA CTGGCCAGGC AGCTGGGCCA GAACTAGGCC TGATGGCTGA TCAGAAGTTA 
CCTGTTGCTC CAGAGCGTGC CACTCGTCCA AGCCAGTCTC CAAAGAAGCC GTTCAATAGC 
ATTATTGAGC ACCTGTCGGT GGTGTTCCCT TGTTACAGCA GCACGGAGCT TGCTGGTTTT 
ATTAAAAAAG TTAGAAACAA AAACAAGACC TCTCTGTCTG GATTGAGTAT TGACGAAATT 
GTGCAAAGAG TGACACAACA CATCCTTGAT GAAGAGAGAA AGAAAAAGCC AAATCCAGGA 
AAGGACCGGA GGCCGTCTGC ACCCAGCTCT GCTGTTCCTG CACCCAGGGC CGCCCAGGAC 
CCGCCCTCCG TGCCTGTCAG AGCATCACCC GAAACCAGGG GGCCGAAGAC CGAAGACGTC 
CCTGTGTCCT GTGCCAATTC CTGTGAAATG TGCCAAGAAG CATTCAAGTC CAAACACGTG 
CGTGAGCTGC AGTGTGGGCA CAGGCTTCAC AAAGGGTGCT TCAAGCAGTG GTTGAAGAGC 
CAGCGCACCT GCCCAGCCTG CCTGGGTCAC GACCTCCTGT CAGAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130619.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986009.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X11, mRNA.

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CloneID	OOv13965
Clone ID Related Accession (Same CDS sequence)	XM_012130619.2 , XM_012130618.2 , XM_012130622.2 , XM_012130621.2
Accession Version	XM_012130619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACG TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CAGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986009.1
CDS	127..5160
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130619.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130619.2

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACG TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CAGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130618.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986008.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

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CloneID	OOv13965
Clone ID Related Accession (Same CDS sequence)	XM_012130619.2 , XM_012130618.2 , XM_012130622.2 , XM_012130621.2
Accession Version	XM_012130618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACG TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CAGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986008.1
CDS	3281..8314
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130618.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130618.2

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACG TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CAGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130622.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986012.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X13, mRNA.

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CloneID	OOv13965
Clone ID Related Accession (Same CDS sequence)	XM_012130619.2 , XM_012130618.2 , XM_012130622.2 , XM_012130621.2
Accession Version	XM_012130622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACG TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CAGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986012.1
CDS	184..5217
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130622.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130622.2

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACG TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CAGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012130621.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986011.1 Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X12, mRNA.

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CloneID	OOv13965
Clone ID Related Accession (Same CDS sequence)	XM_012130619.2 , XM_012130618.2 , XM_012130622.2 , XM_012130621.2
Accession Version	XM_012130621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	E3 ubiquitin-protein ligase TTC3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACG TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CAGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986011.1
CDS	171..5204
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDVKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVRASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012130621.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon tetratricopeptide repeat domain 3 (TTC3), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130621.2

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTCGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACG TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGC TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CAGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_027961634.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027817435.1 Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

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CloneID	OSm59213
Clone ID Related Accession (Same CDS sequence)	XM_027961634.1 , XM_027961627.1 , XM_027961621.1
Accession Version	XM_027961634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TCGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGT TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CGGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027817435.1
CDS	28..5991
Translation	MADFAQGSFSVADYALLEDCPYMDDCVFAPEFMSSDYVRVTQLYCDGVGRQYKDYTQTEGNLEFDICTMWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDMKPQHASANSPQSVRDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVGASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_027961634.1
Organism	Ovis aries(sheep)
Definition	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027961634.1

ORF Insert Sequence:

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TCGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGT TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CGGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_027961627.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027817428.1 Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

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CloneID	OSm59213
Clone ID Related Accession (Same CDS sequence)	XM_027961634.1 , XM_027961627.1 , XM_027961621.1
Accession Version	XM_027961627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TCGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGT TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CGGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027817428.1
CDS	1889..7852
Translation	MADFAQGSFSVADYALLEDCPYMDDCVFAPEFMSSDYVRVTQLYCDGVGRQYKDYTQTEGNLEFDICTMWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDMKPQHASANSPQSVRDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVGASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_027961627.1
Organism	Ovis aries(sheep)
Definition	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027961627.1

ORF Insert Sequence:

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TCGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGT TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CGGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_027961621.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027817422.1 Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

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CloneID	OSm59213
Clone ID Related Accession (Same CDS sequence)	XM_027961634.1 , XM_027961627.1 , XM_027961621.1
Accession Version	XM_027961621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TCGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGT TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CGGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027817422.1
CDS	98..6061
Translation	MADFAQGSFSVADYALLEDCPYMDDCVFAPEFMSSDYVRVTQLYCDGVGRQYKDYTQTEGNLEFDICTMWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDMKPQHASANSPQSVRDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVGASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_027961621.1
Organism	Ovis aries(sheep)
Definition	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027961621.1

ORF Insert Sequence:

ATGGCCGATT TTGCCCAGGG AAGTTTTTCG GTAGCAGATT ATGCCTTGTT AGAAGATTGC 
CCTTACATGG ACGATTGTGT CTTTGCCCCT GAGTTTATGT CCAGTGATTA TGTTCGTGTA 
ACTCAGCTTT ACTGTGATGG AGTGGGTAGA CAGTATAAAG ATTATACCCA AACTGAGGGA 
AACTTAGAAT TCGACATCTG CACTATGTGG TGTAGTAAAC CTGTTTCTGT CCTGCAAGAT 
TACTGTGATG CCATTAAAGT CCACATCTTC TGGCCACTTC TGTTTCAACG TCAGCAGAGC 
TCCATAATAA CACGGATGCA CTCCTGTGTA AGGACCAGCG GTTCTCATGC TTGTGAGATA 
AGTTTGAAGA AATTACAGCA GCTTGAGCTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTTGTGAATG ATACATTCTT TATCGGAGGC TTATTGAGAA TTGGCTATAA AATAGAGAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT CCACAGGCGA TGTAACAATT 
TTATCTAAAT TAGGAGCAGT TGACAATTGT TGGCGTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AATTGTAACA GAAAACTGCA GTTTGCTTGA AGAATTTAAA 
ACTCAAAGTT GTGCGGAGTG TCTAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCCA AAGAAAGATA TGATATAGCT ATTATCTATT ACTCCAGAGC CATTGAATGT 
AGGCCTGAAA ATCACCTGCT TTACGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAGATA GAAGACCTGC AAGGTCGAAC ACCAGCTAGT 
AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA 
ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT 
TGTGACTGTC ATCCTGAATT TGTACCACCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA 
CAAAAAGCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA 
GTAGATTTGA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC TGCACACCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGCTA CACGGCCTTG 
CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG GCCTTCACAG AGTTGCTCAA CGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAATCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA 
CTTGCCATTT CACTCCTTGG AATAGGACAG CCTGAGGAGT TATCTGAAGC TGAAAACCAA 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGCTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACGAG TAATGTGATT 
ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AAAAATTCGT TGAAGAATGC 
AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA 
ATTCAGATAT ATATAACCGA TCCAGACTTC AAGGGTTTTA TACGAATCAG CTGTTGCCAG 
TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT 
GATAAAATTG ACAAGGATTT TCTGCAAGGA ATTTGTCTTA CTCCTGATTG TAAAGGAATC 
ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATTAAAG AAAAGCTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAACGAAAGC TCCAAAAAAA GGAAGCAAAA 
AAATTAGCAC AAGAAAGACT GGAAGAGGAC CTAAGGGAAA GTAACCCACC AAAAACTGAA 
GAGCAGAGAG AAACTATCAG CCGTGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT 
AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTTATTC GTCATTTGAT CCAGGAAAAG 
AACAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA 
AAACTAGCCA CCTGGATCCA GAAACTTAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC 
TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT 
GAGCCTACGT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTGGAAGA ACACAGAGAC 
AAGTTCCCGG CACTGCATAA TGCCCTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC 
ACTGTGTTAA GGAAACAGGA CAGTGGTGAC GTGCCATGTA ATTTTACCAA GATAAAAAAC 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT 
TCAGTCACAC CAAACAATGA GACTTTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA 
AATTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATGTAGA AGAATTTGAA 
GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA 
AACCCAAAAC AAAAATGTTC AACTCTATAT GATTATTTCT CTCAGTTGTT GGAGGAACAT 
GGTCCCTTGG ACATGAGTAA CAAGATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA 
ACTCGACAGA TACTAGAAAA AGCAGGAGGT TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAGTGTCTG ACTTACCTTT AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCATGCATCT 
GCTAATTCTC CCCAATCAGT TCGTGACACT GGGAAGCCAA AACCAGTTTC CGATAATTCT 
TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATAT CTTCTGATTC TCCCAGGCCA 
GTCCTTGAGG AGACCATTCA CAAGCCAGTC TCCTCTAATT CTCCCAAACC AGTTCCCGAG 
GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT 
CCCTTTCGAG GATTTGACAT TACCCAGACA CCACCAGCCT TCATCAGTGT GTTACCGACT 
TTGCCCCAGT ATAGCATTTA CACCCCCCTG GCCAGCGTTC CTACTGAGTA TCACCTGCAA 
AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG 
GCTCATCATT TCAGTGCTGA GAATGCCCAT GGTAACCAGA TTGCCTCCGA AACACAGATC 
CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTCTGATGG TGCTGATATA 
GCCCAGAGTG AGGCTAGCGG AAACGAGGAC TACTGTGGAG ATTCTGCCAA GTGGGAAGTA 
AATCCAGAAA GCACCAATAA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG 
GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA 
CAACAGGGAG GTATTTCACA GATTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG 
GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAGGGGCT CAGAAGAAAC CAAGGACTTG 
GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG 
TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA 
GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TGAATGCCAG CAGAATGTAT 
ACACAGGAAA ACGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA 
ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA 
GAGCATTATG AAGAGAGTCT TCAGAGGGCT CTGGATGCAG AGGTATCTGT GCTTGAAAAC 
TGGAAGGATG TGGAGGTGTG TAAGTTACAG AGCATGGAGT CACAGGCCGA AGGGCTTCTC 
AAGAACTTGA AGCTAATGAG CAGTGATTCG GCCGCATATT CTGACATGGA CTCAGACATA 
CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG 
TTTGAAGAAC AAATTCAGGC AATTAAAAAT GGTTCTCGGC TCAGTGAACT TCCTAAAGTG 
CAGATTTCTG AGCTTTCGTT TCCTACCTGT AACACAATTC ATCCTAAATT ACTTCCTGAG 
TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC ACTTCTGCAA GTCATGGGAC CGGAGACCAG 
ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC 
TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA GAGCGTGAGG GCACTGGCCA GGCAGCTGGG 
CCAGAACTAG GCCTGATGGC TGATCAGAAG TTACCTGTTG CTCCAGAGCG TGCCACTCGT 
CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTC GGTGGTGTTC 
CCTTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG 
ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACACA ACACATCCTT 
GATGAAGAGA GAAAGAAAAA GCCAAATCCA GGAAAGGACC GGAGGCCGTC TGCACCCAGC 
TCTGCTGTTC CTGCACCCAG GGCCGCCCAG GACCCGCCCT CCGTGCCTGT CGGAGCATCA 
CCCGAAACCA GGGGGCCGAA GACCGAAGAC GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA 
ATGTGCCAAG AAGCATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT 
CACAAAGGGT GCTTCAAGCA GTGGTTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT 
CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_027961639.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027817440.1 Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

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CloneID	OSm59214
Clone ID Related Accession (Same CDS sequence)	XM_027961639.1
Accession Version	XM_027961639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTA GTAAACCTGT TTCTGTCCTG CAAGATTACT GTGATGCCAT TAAAGTCCAC 
ATCTTCTGGC CACTTCTGTT TCAACGTCAG CAGAGCTCCA TAATAACACG GATGCACTCC 
TGTGTAAGGA CCAGCGGTTC TCATGCTTGT GAGATAAGTT TGAAGAAATT ACAGCAGCTT 
GAGCTGATGG AAGATATTGT GGATTTGGCA AAGAAAGTTG TGAATGATAC ATTCTTTATC 
GGAGGCTTAT TGAGAATTGG CTATAAAATA GAGAATAAAA TCTTGGCAAT GGAAGAAGCT 
TTGAATTGGG TGAAATCCAC AGGCGATGTA ACAATTTTAT CTAAATTAGG AGCAGTTGAC 
AATTGTTGGC GTATGTTAAG TATTTTCTTT ACTGAATACA AGTATCACAT AACTAAAATT 
GTAACAGAAA ACTGCAGTTT GCTTGAAGAA TTTAAAACTC AAAGTTGTGC GGAGTGTCTA 
GAGCAAGGAG AACTAATGAA AATGAAAGGA AATGAAGAGT TTTCCAAAGA AAGATATGAT 
ATAGCTATTA TCTATTACTC CAGAGCCATT GAATGTAGGC CTGAAAATCA CCTGCTTTAC 
GGTAACCGAG CTCTTTGTTT TCTTCGTACT GGACAATTCA GAAATGCACT TGGTGATGGA 
AAGAGAGCTA CCATTCTGAA GAACAATTGG ACAAAGGGTC ATTATCGTTA TTGTGCTGCT 
CTTTCTATGC TGGGGGAATA TGACTGGGCC CTGCAAGCAA ACATAAAAGC TCAAAAACTC 
TGTAAAAATG ACCCTGAGGG AATCAAGGAT CTAATTCAGC AGCATGTAAA GTTACAAAAA 
CAGATAGAAG ACCTGCAAGG TCGAACACCA GCTAGTAATC CAGTCAAAGC CTTCTGTGAA 
AGCAGGGCCT ACATACCTAG TTTATCAGCA CCTGCATTTA GTACTTCACT TAACTTTGTG 
GAAACTGAAA AAAATTTCAG AAAAACTAAC CACGAAATGG CAAACGGTGG TAATCAGAAT 
CCAAAGGTGG TAGATGAAGC TCTGAAAGTA GATGATTGTG ACTGTCATCC TGAATTTGTA 
CCACCATCAA GTCAGCCTCC AAAATATAAA GGGAAACAAA AAGCTCGAAA CAATGAATTG 
GAGAAGTTCA GCCCTGGTTC ACAAGAAACT TTACCAGTAG ATTTGAAAAA CATCTTGGAG 
AAACAGTTTT CTAAATCTTC CAGAGCTGCA CACCAGGATT TTGCTAATAT AATGAAAATG 
CTGAGAAGCT TAATCCAGGA TGGCTACACG GCCTTGCTGG AGCAGCGCTG CCGCAGTGCT 
GCCCAGGCCT TCACAGAGTT GCTCAACGGT TTAGATCCTC AAAAAATAAA GCAATTGAAT 
CTGGCCATGA TTAACTACGT GTTAGTAGTC TACGGACTTG CCATTTCACT CCTTGGAATA 
GGACAGCCTG AGGAGTTATC TGAAGCTGAA AACCAATTTA AGAGGATGAT TGAACACTAC 
CCCAATGAGG GACTTGATTG CTTGGCCTAC TGTGGAATTG GAAAAGTATA TTTGAAAAAA 
AACAGATTTT TAGAAGCTCT TAATCATTTT GAGAAAGCAA GAACCTTGAT CTGTCGTCTT 
CCTGGAGTGT TAACTTGGCC CACGAGTAAT GTGATTATTG AGGAGACTCA GCCAGGGAAG 
ATAAAGATGC TGTTAGAAAA ATTCGTTGAA GAATGCAGGT TCCCTCCAGT GCCGGATGCC 
ACTTGTTGCT ATCAGAAGTG CCATGGATAT TCCAAAATTC AGATATATAT AACCGATCCA 
GACTTCAAGG GTTTTATACG AATCAGCTGT TGCCAGTATT GTAAAATAGA ATTTCACATG 
AACTGCTGGA AGAAGTTAAA AACAACAACC TTCAATGATA AAATTGACAA GGATTTTCTG 
CAAGGAATTT GTCTTACTCC TGATTGTAAA GGAATCATTT CAAAGATTAT CATCTTCAGC 
AGTGGTGGTC AAGTTAAATG TGAATTTGAA CACAAGGTCA TTAAAGAAAA GCTTCCTCCA 
AGACCTATTC TGAAACAGAA ATGTTCTAGC CTAGAGAAGT TGAGGCTGAA AGAAGACAAA 
AAATTGAAAC GAAAGCTCCA AAAAAAGGAA GCAAAAAAAT TAGCACAAGA AAGACTGGAA 
GAGGACCTAA GGGAAAGTAA CCCACCAAAA ACTGAAGAGC AGAGAGAAAC TATCAGCCGT 
GTCCAAAGTT GCCAGTTCCT TGATGACAGA ATTCTGCAGT GCATAAAGCA GTACGCTGAC 
AAGATTAAGT CCGGGATACT GAATACTTCC AAGCTTCTCA AAGAATTGCT TTCTTGGAAG 
GTTTTAACCA CAGAAGACTA CACAACGTGT TTTTCTAGCA GAAATTTCCT GAATGAAGCA 
GTGGACTATG TTATTCGTCA TTTGATCCAG GAAAAGAACA GAGTAAAGAC AAGGGTATTT 
CTGCATGTTT TGAGTGAGCT TAAAGAAGTG GAGCCAAAAC TAGCCACCTG GATCCAGAAA 
CTTAATAGCT TTGGCTTAGA TGCCACAGGA CCTTTCTTTT CTCGTTATGG GGCTCCTCTT 
AAGGAGCTTG ATTTTAGCAT CATGACTTTT CTCTGGAATG AGAAATATGG TCATAAGCTA 
GGCTCTATAG AAGGAAAGCA ACTTGATTAT TTCTATGAGC CTACGTCACT GAAGGAAGCG 
CGGTGTTTAA TATGGCTGCT GGAAGAACAC AGAGACAAGT TCCCGGCACT GCATAATGCC 
CTGGACGAGT TCTTTGATAT AATGGACAGC CGCTGCACTG TGTTAAGGAA ACAGGACAGT 
GGTGACGTGC CATGTAATTT TACCAAGATA AAAAACAAAG GCAAGAAAAA GAAGCCAAAA 
GATTCAAAGC CTATGTTAGT TGGATCTGGA ACACCTTCAG TCACACCAAA CAATGAGACT 
TTCACTTCAG GTGAAGACCA TATCAAACGC AGTTTAAATT CTGCGGGCCC CTTTGTAGTG 
CCTGATCACC TTCGGCAGGA TGTAGAAGAA TTTGAAGCTC TGTATGAACA GCATAGTAAT 
GAGTATGTTG TCCGTAATAA GAAGCTGTGG GACATAAACC CAAAACAAAA ATGTTCAACT 
CTATATGATT ATTTCTCTCA GTTGTTGGAG GAACATGGTC CCTTGGACAT GAGTAACAAG 
ATGTTTTCTG AAGAATATGA GTTCTTCCCA GAAGAAACTC GACAGATACT AGAAAAAGCA 
GGAGGTTTGA AAGCGTTTCT CCTGGGATGT CCTCGTTTTG TTGTGATTGA CAACTGTATT 
GCATTGAAGA AAGTTGCATT ACGGCTCAAG AAAAAGAGAA AGAAGAAAAA CATTAAGACA 
AAAGTAGAAG AAATTTCAAA AACAGGAGAG TATTTACGAG TTAAACTACC ACTGAATCCA 
ACTGCTAGGG AATTTAAACC AGATGTAAAG TCCAAACCAG TGTCTGACTT ACCTTTAGCA 
CCAGCTTCTG AGGACATGAA GCCTCAGCAT GCATCTGCTA ATTCTCCCCA ATCAGTTCGT 
GACACTGGGA AGCCAAAACC AGTTTCCGAT AATTCTTCCA GATCAGTTTC TGAGGATGAG 
AAGCCCAAAG GGATATCTTC TGATTCTCCC AGGCCAGTCC TTGAGGAGAC CATTCACAAG 
CCAGTCTCCT CTAATTCTCC CAAACCAGTT CCCGAGGAAG TGAAAGCGAC CTATTGGCCT 
CGACCCCAGT TGGTCACAGG ATACTGTACA TATCTTCCCT TTCGAGGATT TGACATTACC 
CAGACACCAC CAGCCTTCAT CAGTGTGTTA CCGACTTTGC CCCAGTATAG CATTTACACC 
CCCCTGGCCA GCGTTCCTAC TGAGTATCAC CTGCAAAGAC CAATACCAGT GGTGCCGTCT 
TTTGTAGCCA GTGACATAAC GGAAGTGCAT TTTGAGGCTC ATCATTTCAG TGCTGAGAAT 
GCCCATGGTA ACCAGATTGC CTCCGAAACA CAGATCCTTG AGGAATCTTT GGAAACACCA 
GTGAAGTCAG AGTGCAGCTC TGATGGTGCT GATATAGCCC AGAGTGAGGC TAGCGGAAAC 
GAGGACTACT GTGGAGATTC TGCCAAGTGG GAAGTAAATC CAGAAAGCAC CAATAAAGTA 
ACAAATATTC CATGTACACA AGTGGTTGCT GTACAGGTGT CTTGGAATAT AACACATCAG 
GAAGTCAATA CTGAGCCATA TGGTCCTTTT GAGGCACAAC AGGGAGGTAT TTCACAGATT 
GAAAAGGAGT ACCAGGTTTT ACAAGAGCAG CTTAAGGAAG CATGTGAAAA TTATGAACAC 
AGCAATGTCA GGGGCTCAGA AGAAACCAAG GACTTGGAAG AAAAGTTGAA AAGGAACGTT 
GAAGAAAATA AGATCTCAAA GACAGAACTA GATTGGTTCC TTGAAGATTT GAACAAGGAA 
ATTAAAAAAT GGGAACAGGA GAAAAAAGAA ATCCAAGAAA GACTAAAGGC ACTGAAGAAG 
AAAATGAAAA AGGTGTTGAA TGCCAGCAGA ATGTATACAC AGGAAAACGA TGGAAAGGAA 
AAGGCACTTG AATTACTTCT GGATCAGTCC CTTGAAATCA GTAATACATT TACAAATGAG 
AAAATGAAAA TAGAAGAGAG TATAAAGAAA GGGAAAGAGC ATTATGAAGA GAGTCTTCAG 
AGGGCTCTGG ATGCAGAGGT ATCTGTGCTT GAAAACTGGA AGGATGTGGA GGTGTGTAAG 
TTACAGAGCA TGGAGTCACA GGCCGAAGGG CTTCTCAAGA ACTTGAAGCT AATGAGCAGT 
GATTCGGCCG CATATTCTGA CATGGACTCA GACATACATT CATGGGAATC GTTTCTTTCT 
GATGTTAGAG AAGAAATTGA GAAAGCAAAG TCTCAGTTTG AAGAACAAAT TCAGGCAATT 
AAAAATGGTT CTCGGCTCAG TGAACTTCCT AAAGTGCAGA TTTCTGAGCT TTCGTTTCCT 
ACCTGTAACA CAATTCATCC TAAATTACTT CCTGAGTCTT CAGGCCAGGA GGGTCAAGGT 
CCCTCCACTT CTGCAAGTCA TGGGACCGGA GACCAGACAG CAGCTCCCGA GGAGTCAAGT 
CTGGGTTCTG CTGCTGACAG CCCAGTGGAG TCGCCCTCCT GCAGCCAGAA AGCCCCCCAG 
CTGCCAGAGC GTGAGGGCAC TGGCCAGGCA GCTGGGCCAG AACTAGGCCT GATGGCTGAT 
CAGAAGTTAC CTGTTGCTCC AGAGCGTGCC ACTCGTCCAA GCCAGTCTCC AAAGAAGCCG 
TTCAATAGCA TTATTGAGCA CCTGTCGGTG GTGTTCCCTT GTTACAGCAG CACGGAGCTT 
GCTGGTTTTA TTAAAAAAGT TAGAAACAAA AACAAGACCT CTCTGTCTGG ATTGAGTATT 
GACGAAATTG TGCAAAGAGT GACACAACAC ATCCTTGATG AAGAGAGAAA GAAAAAGCCA 
AATCCAGGAA AGGACCGGAG GCCGTCTGCA CCCAGCTCTG CTGTTCCTGC ACCCAGGGCC 
GCCCAGGACC CGCCCTCCGT GCCTGTCGGA GCATCACCCG AAACCAGGGG GCCGAAGACC 
GAAGACGTCC CTGTGTCCTG TGCCAATTCC TGTGAAATGT GCCAAGAAGC ATTCAAGTCC 
AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AAGGGTGCTT CAAGCAGTGG 
TTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027817440.1
CDS	88..5847
Translation	MWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDMKPQHASANSPQSVRDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVGASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_027961639.1
Organism	Ovis aries(sheep)
Definition	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027961639.1

ORF Insert Sequence:

ATGTGGTGTA GTAAACCTGT TTCTGTCCTG CAAGATTACT GTGATGCCAT TAAAGTCCAC 
ATCTTCTGGC CACTTCTGTT TCAACGTCAG CAGAGCTCCA TAATAACACG GATGCACTCC 
TGTGTAAGGA CCAGCGGTTC TCATGCTTGT GAGATAAGTT TGAAGAAATT ACAGCAGCTT 
GAGCTGATGG AAGATATTGT GGATTTGGCA AAGAAAGTTG TGAATGATAC ATTCTTTATC 
GGAGGCTTAT TGAGAATTGG CTATAAAATA GAGAATAAAA TCTTGGCAAT GGAAGAAGCT 
TTGAATTGGG TGAAATCCAC AGGCGATGTA ACAATTTTAT CTAAATTAGG AGCAGTTGAC 
AATTGTTGGC GTATGTTAAG TATTTTCTTT ACTGAATACA AGTATCACAT AACTAAAATT 
GTAACAGAAA ACTGCAGTTT GCTTGAAGAA TTTAAAACTC AAAGTTGTGC GGAGTGTCTA 
GAGCAAGGAG AACTAATGAA AATGAAAGGA AATGAAGAGT TTTCCAAAGA AAGATATGAT 
ATAGCTATTA TCTATTACTC CAGAGCCATT GAATGTAGGC CTGAAAATCA CCTGCTTTAC 
GGTAACCGAG CTCTTTGTTT TCTTCGTACT GGACAATTCA GAAATGCACT TGGTGATGGA 
AAGAGAGCTA CCATTCTGAA GAACAATTGG ACAAAGGGTC ATTATCGTTA TTGTGCTGCT 
CTTTCTATGC TGGGGGAATA TGACTGGGCC CTGCAAGCAA ACATAAAAGC TCAAAAACTC 
TGTAAAAATG ACCCTGAGGG AATCAAGGAT CTAATTCAGC AGCATGTAAA GTTACAAAAA 
CAGATAGAAG ACCTGCAAGG TCGAACACCA GCTAGTAATC CAGTCAAAGC CTTCTGTGAA 
AGCAGGGCCT ACATACCTAG TTTATCAGCA CCTGCATTTA GTACTTCACT TAACTTTGTG 
GAAACTGAAA AAAATTTCAG AAAAACTAAC CACGAAATGG CAAACGGTGG TAATCAGAAT 
CCAAAGGTGG TAGATGAAGC TCTGAAAGTA GATGATTGTG ACTGTCATCC TGAATTTGTA 
CCACCATCAA GTCAGCCTCC AAAATATAAA GGGAAACAAA AAGCTCGAAA CAATGAATTG 
GAGAAGTTCA GCCCTGGTTC ACAAGAAACT TTACCAGTAG ATTTGAAAAA CATCTTGGAG 
AAACAGTTTT CTAAATCTTC CAGAGCTGCA CACCAGGATT TTGCTAATAT AATGAAAATG 
CTGAGAAGCT TAATCCAGGA TGGCTACACG GCCTTGCTGG AGCAGCGCTG CCGCAGTGCT 
GCCCAGGCCT TCACAGAGTT GCTCAACGGT TTAGATCCTC AAAAAATAAA GCAATTGAAT 
CTGGCCATGA TTAACTACGT GTTAGTAGTC TACGGACTTG CCATTTCACT CCTTGGAATA 
GGACAGCCTG AGGAGTTATC TGAAGCTGAA AACCAATTTA AGAGGATGAT TGAACACTAC 
CCCAATGAGG GACTTGATTG CTTGGCCTAC TGTGGAATTG GAAAAGTATA TTTGAAAAAA 
AACAGATTTT TAGAAGCTCT TAATCATTTT GAGAAAGCAA GAACCTTGAT CTGTCGTCTT 
CCTGGAGTGT TAACTTGGCC CACGAGTAAT GTGATTATTG AGGAGACTCA GCCAGGGAAG 
ATAAAGATGC TGTTAGAAAA ATTCGTTGAA GAATGCAGGT TCCCTCCAGT GCCGGATGCC 
ACTTGTTGCT ATCAGAAGTG CCATGGATAT TCCAAAATTC AGATATATAT AACCGATCCA 
GACTTCAAGG GTTTTATACG AATCAGCTGT TGCCAGTATT GTAAAATAGA ATTTCACATG 
AACTGCTGGA AGAAGTTAAA AACAACAACC TTCAATGATA AAATTGACAA GGATTTTCTG 
CAAGGAATTT GTCTTACTCC TGATTGTAAA GGAATCATTT CAAAGATTAT CATCTTCAGC 
AGTGGTGGTC AAGTTAAATG TGAATTTGAA CACAAGGTCA TTAAAGAAAA GCTTCCTCCA 
AGACCTATTC TGAAACAGAA ATGTTCTAGC CTAGAGAAGT TGAGGCTGAA AGAAGACAAA 
AAATTGAAAC GAAAGCTCCA AAAAAAGGAA GCAAAAAAAT TAGCACAAGA AAGACTGGAA 
GAGGACCTAA GGGAAAGTAA CCCACCAAAA ACTGAAGAGC AGAGAGAAAC TATCAGCCGT 
GTCCAAAGTT GCCAGTTCCT TGATGACAGA ATTCTGCAGT GCATAAAGCA GTACGCTGAC 
AAGATTAAGT CCGGGATACT GAATACTTCC AAGCTTCTCA AAGAATTGCT TTCTTGGAAG 
GTTTTAACCA CAGAAGACTA CACAACGTGT TTTTCTAGCA GAAATTTCCT GAATGAAGCA 
GTGGACTATG TTATTCGTCA TTTGATCCAG GAAAAGAACA GAGTAAAGAC AAGGGTATTT 
CTGCATGTTT TGAGTGAGCT TAAAGAAGTG GAGCCAAAAC TAGCCACCTG GATCCAGAAA 
CTTAATAGCT TTGGCTTAGA TGCCACAGGA CCTTTCTTTT CTCGTTATGG GGCTCCTCTT 
AAGGAGCTTG ATTTTAGCAT CATGACTTTT CTCTGGAATG AGAAATATGG TCATAAGCTA 
GGCTCTATAG AAGGAAAGCA ACTTGATTAT TTCTATGAGC CTACGTCACT GAAGGAAGCG 
CGGTGTTTAA TATGGCTGCT GGAAGAACAC AGAGACAAGT TCCCGGCACT GCATAATGCC 
CTGGACGAGT TCTTTGATAT AATGGACAGC CGCTGCACTG TGTTAAGGAA ACAGGACAGT 
GGTGACGTGC CATGTAATTT TACCAAGATA AAAAACAAAG GCAAGAAAAA GAAGCCAAAA 
GATTCAAAGC CTATGTTAGT TGGATCTGGA ACACCTTCAG TCACACCAAA CAATGAGACT 
TTCACTTCAG GTGAAGACCA TATCAAACGC AGTTTAAATT CTGCGGGCCC CTTTGTAGTG 
CCTGATCACC TTCGGCAGGA TGTAGAAGAA TTTGAAGCTC TGTATGAACA GCATAGTAAT 
GAGTATGTTG TCCGTAATAA GAAGCTGTGG GACATAAACC CAAAACAAAA ATGTTCAACT 
CTATATGATT ATTTCTCTCA GTTGTTGGAG GAACATGGTC CCTTGGACAT GAGTAACAAG 
ATGTTTTCTG AAGAATATGA GTTCTTCCCA GAAGAAACTC GACAGATACT AGAAAAAGCA 
GGAGGTTTGA AAGCGTTTCT CCTGGGATGT CCTCGTTTTG TTGTGATTGA CAACTGTATT 
GCATTGAAGA AAGTTGCATT ACGGCTCAAG AAAAAGAGAA AGAAGAAAAA CATTAAGACA 
AAAGTAGAAG AAATTTCAAA AACAGGAGAG TATTTACGAG TTAAACTACC ACTGAATCCA 
ACTGCTAGGG AATTTAAACC AGATGTAAAG TCCAAACCAG TGTCTGACTT ACCTTTAGCA 
CCAGCTTCTG AGGACATGAA GCCTCAGCAT GCATCTGCTA ATTCTCCCCA ATCAGTTCGT 
GACACTGGGA AGCCAAAACC AGTTTCCGAT AATTCTTCCA GATCAGTTTC TGAGGATGAG 
AAGCCCAAAG GGATATCTTC TGATTCTCCC AGGCCAGTCC TTGAGGAGAC CATTCACAAG 
CCAGTCTCCT CTAATTCTCC CAAACCAGTT CCCGAGGAAG TGAAAGCGAC CTATTGGCCT 
CGACCCCAGT TGGTCACAGG ATACTGTACA TATCTTCCCT TTCGAGGATT TGACATTACC 
CAGACACCAC CAGCCTTCAT CAGTGTGTTA CCGACTTTGC CCCAGTATAG CATTTACACC 
CCCCTGGCCA GCGTTCCTAC TGAGTATCAC CTGCAAAGAC CAATACCAGT GGTGCCGTCT 
TTTGTAGCCA GTGACATAAC GGAAGTGCAT TTTGAGGCTC ATCATTTCAG TGCTGAGAAT 
GCCCATGGTA ACCAGATTGC CTCCGAAACA CAGATCCTTG AGGAATCTTT GGAAACACCA 
GTGAAGTCAG AGTGCAGCTC TGATGGTGCT GATATAGCCC AGAGTGAGGC TAGCGGAAAC 
GAGGACTACT GTGGAGATTC TGCCAAGTGG GAAGTAAATC CAGAAAGCAC CAATAAAGTA 
ACAAATATTC CATGTACACA AGTGGTTGCT GTACAGGTGT CTTGGAATAT AACACATCAG 
GAAGTCAATA CTGAGCCATA TGGTCCTTTT GAGGCACAAC AGGGAGGTAT TTCACAGATT 
GAAAAGGAGT ACCAGGTTTT ACAAGAGCAG CTTAAGGAAG CATGTGAAAA TTATGAACAC 
AGCAATGTCA GGGGCTCAGA AGAAACCAAG GACTTGGAAG AAAAGTTGAA AAGGAACGTT 
GAAGAAAATA AGATCTCAAA GACAGAACTA GATTGGTTCC TTGAAGATTT GAACAAGGAA 
ATTAAAAAAT GGGAACAGGA GAAAAAAGAA ATCCAAGAAA GACTAAAGGC ACTGAAGAAG 
AAAATGAAAA AGGTGTTGAA TGCCAGCAGA ATGTATACAC AGGAAAACGA TGGAAAGGAA 
AAGGCACTTG AATTACTTCT GGATCAGTCC CTTGAAATCA GTAATACATT TACAAATGAG 
AAAATGAAAA TAGAAGAGAG TATAAAGAAA GGGAAAGAGC ATTATGAAGA GAGTCTTCAG 
AGGGCTCTGG ATGCAGAGGT ATCTGTGCTT GAAAACTGGA AGGATGTGGA GGTGTGTAAG 
TTACAGAGCA TGGAGTCACA GGCCGAAGGG CTTCTCAAGA ACTTGAAGCT AATGAGCAGT 
GATTCGGCCG CATATTCTGA CATGGACTCA GACATACATT CATGGGAATC GTTTCTTTCT 
GATGTTAGAG AAGAAATTGA GAAAGCAAAG TCTCAGTTTG AAGAACAAAT TCAGGCAATT 
AAAAATGGTT CTCGGCTCAG TGAACTTCCT AAAGTGCAGA TTTCTGAGCT TTCGTTTCCT 
ACCTGTAACA CAATTCATCC TAAATTACTT CCTGAGTCTT CAGGCCAGGA GGGTCAAGGT 
CCCTCCACTT CTGCAAGTCA TGGGACCGGA GACCAGACAG CAGCTCCCGA GGAGTCAAGT 
CTGGGTTCTG CTGCTGACAG CCCAGTGGAG TCGCCCTCCT GCAGCCAGAA AGCCCCCCAG 
CTGCCAGAGC GTGAGGGCAC TGGCCAGGCA GCTGGGCCAG AACTAGGCCT GATGGCTGAT 
CAGAAGTTAC CTGTTGCTCC AGAGCGTGCC ACTCGTCCAA GCCAGTCTCC AAAGAAGCCG 
TTCAATAGCA TTATTGAGCA CCTGTCGGTG GTGTTCCCTT GTTACAGCAG CACGGAGCTT 
GCTGGTTTTA TTAAAAAAGT TAGAAACAAA AACAAGACCT CTCTGTCTGG ATTGAGTATT 
GACGAAATTG TGCAAAGAGT GACACAACAC ATCCTTGATG AAGAGAGAAA GAAAAAGCCA 
AATCCAGGAA AGGACCGGAG GCCGTCTGCA CCCAGCTCTG CTGTTCCTGC ACCCAGGGCC 
GCCCAGGACC CGCCCTCCGT GCCTGTCGGA GCATCACCCG AAACCAGGGG GCCGAAGACC 
GAAGACGTCC CTGTGTCCTG TGCCAATTCC TGTGAAATGT GCCAAGAAGC ATTCAAGTCC 
AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AAGGGTGCTT CAAGCAGTGG 
TTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_027961647.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027817448.1 Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSm59215
Clone ID Related Accession (Same CDS sequence)	XM_027961647.1
Accession Version	XM_027961647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGT TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CGGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027817448.1
CDS	332..5365
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDMKPQHASANSPQSVRDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVGASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_027961647.1
Organism	Ovis aries(sheep)
Definition	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027961647.1

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAGATA 
GAAGACCTGC AAGGTCGAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG 
GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCACCA 
TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAAGCTC GAAACAATGA ATTGGAGAAG 
TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTGA AAAACATCTT GGAGAAACAG 
TTTTCTAAAT CTTCCAGAGC TGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG TGCTGCCCAG 
GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAATCTGGCC 
ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG 
CCTGAGGAGT TATCTGAAGC TGAAAACCAA TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAAAACAGA 
TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACGAG TAATGTGATT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG 
ATGCTGTTAG AAAAATTCGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT 
TGCTATCAGA AGTGCCATGG ATATTCCAAA ATTCAGATAT ATATAACCGA TCCAGACTTC 
AAGGGTTTTA TACGAATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC 
TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA 
ATTTGTCTTA CTCCTGATTG TAAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGCTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG 
AAACGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC 
CTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAG CCGTGTCCAA 
AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT 
AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC 
TATGTTATTC GTCATTTGAT CCAGGAAAAG AACAGAGTAA AGACAAGGGT ATTTCTGCAT 
GTTTTGAGTG AGCTTAAAGA AGTGGAGCCA AAACTAGCCA CCTGGATCCA GAAACTTAAT 
AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA GCTAGGCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACGT CACTGAAGGA AGCGCGGTGT 
TTAATATGGC TGCTGGAAGA ACACAGAGAC AAGTTCCCGG CACTGCATAA TGCCCTGGAC 
GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTGTTAA GGAAACAGGA CAGTGGTGAC 
GTGCCATGTA ATTTTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTTTCACT 
TCAGGTGAAG ACCATATCAA ACGCAGTTTA AATTCTGCGG GCCCCTTTGT AGTGCCTGAT 
CACCTTCGGC AGGATGTAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT 
GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AAAAATGTTC AACTCTATAT 
GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAGATGTTT 
TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCGACAGA TACTAGAAAA AGCAGGAGGT 
TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCATTG 
AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTA 
GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTT AGCACCAGCT 
TCTGAGGACA TGAAGCCTCA GCATGCATCT GCTAATTCTC CCCAATCAGT TCGTGACACT 
GGGAAGCCAA AACCAGTTTC CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC 
AAAGGGATAT CTTCTGATTC TCCCAGGCCA GTCCTTGAGG AGACCATTCA CAAGCCAGTC 
TCCTCTAATT CTCCCAAACC AGTTCCCGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC 
CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGAG GATTTGACAT TACCCAGACA 
CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCCCTG 
GCCAGCGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA 
GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCAT 
GGTAACCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG 
TCAGAGTGCA GCTCTGATGG TGCTGATATA GCCCAGAGTG AGGCTAGCGG AAACGAGGAC 
TACTGTGGAG ATTCTGCCAA GTGGGAAGTA AATCCAGAAA GCACCAATAA AGTAACAAAT 
ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC 
AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGAG GTATTTCACA GATTGAAAAG 
GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT 
GTCAGGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA CGTTGAAGAA 
AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA 
AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG 
AAAAAGGTGT TGAATGCCAG CAGAATGTAT ACACAGGAAA ACGATGGAAA GGAAAAGGCA 
CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG 
AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAGAGGGCT 
CTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGATG TGGAGGTGTG TAAGTTACAG 
AGCATGGAGT CACAGGCCGA AGGGCTTCTC AAGAACTTGA AGCTAATGAG CAGTGATTCG 
GCCGCATATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT 
AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAAAAT 
GGTTCTCGGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCGTT TCCTACCTGT 
AACACAATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGAGGGTCA AGGTCCCTCC 
ACTTCTGCAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGT 
TCTGCTGCTG ACAGCCCAGT GGAGTCGCCC TCCTGCAGCC AGAAAGCCCC CCAGCTGCCA 
GAGCGTGAGG GCACTGGCCA GGCAGCTGGG CCAGAACTAG GCCTGATGGC TGATCAGAAG 
TTACCTGTTG CTCCAGAGCG TGCCACTCGT CCAAGCCAGT CTCCAAAGAA GCCGTTCAAT 
AGCATTATTG AGCACCTGTC GGTGGTGTTC CCTTGTTACA GCAGCACGGA GCTTGCTGGT 
TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA 
ATTGTGCAAA GAGTGACACA ACACATCCTT GATGAAGAGA GAAAGAAAAA GCCAAATCCA 
GGAAAGGACC GGAGGCCGTC TGCACCCAGC TCTGCTGTTC CTGCACCCAG GGCCGCCCAG 
GACCCGCCCT CCGTGCCTGT CGGAGCATCA CCCGAAACCA GGGGGCCGAA GACCGAAGAC 
GTCCCTGTGT CCTGTGCCAA TTCCTGTGAA ATGTGCCAAG AAGCATTCAA GTCCAAACAC 
GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAAGGGT GCTTCAAGCA GTGGTTGAAG 
AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_012105081.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960471.1 Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm37457
Clone ID Related Accession (Same CDS sequence)	XM_012105081.2
Accession Version	XM_012105081.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012105081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACCAA CGGTTTGCTA CAAAGGGTGG TGTACCATGG CCGATTTTGC CCAGGGAAGT 
TTTTCGGTAG CAGATTATGC CTTGTTAGAA GATTGCCCTT ACATGGACGA TTGTGTCTTT 
GCCCCTGAGT TTATGTCCAG TGATTATGTT CGTGTAACTC AGCTTTACTG TGATGGAGTG 
GGTAGACAGT ATAAAGATTA TACCCAAACT GAGGGAAACT TAGAATTCGA CATCTGCACT 
ATGTGGTGTA GTAAACCTGT TTCTGTCCTG CAAGATTACT GTGATGCCAT TAAAGTCCAC 
ATCTTCTGGC CACTTCTGTT TCAACGTCAG CAGAGCTCCA TAATAACACG GATGCACTCC 
TGTGTAAGGA CCAGCGGTTC TCATGCTTGT GAGATAAGTT TGAAGAAATT ACAGCAGCTT 
GAGCTGATGG AAGATATTGT GGATTTGGCA AAGAAAGTTG TGAATGATAC ATTCTTTATC 
GGAGGCTTAT TGAGAATTGG CTATAAAATA GAGAATAAAA TCTTGGCAAT GGAAGAAGCT 
TTGAATTGGG TGAAATCCAC AGGCGATGTA ACAATTTTAT CTAAATTAGG AGCAGTTGAC 
AATTGTTGGC GTATGTTAAG TATTTTCTTT ACTGAATACA AGTATCACAT AACTAAAATT 
GTAACAGAAA ACTGCAGTTT GCTTGAAGAA TTTAAAACTC AAAGTTGTGC GGAGTGTCTA 
GAGCAAGGAG AACTAATGAA AATGAAAGGA AATGAAGAGT TTTCCAAAGA AAGATATGAT 
ATAGCTATTA TCTATTACTC CAGAGCCATT GAATGTAGGC CTGAAAATCA CCTGCTTTAC 
GGTAACCGAG CTCTTTGTTT TCTTCGTACT GGACAATTCA GAAATGCACT TGGTGATGGA 
AAGAGAGCTA CCATTCTGAA GAACAATTGG ACAAAGGGTC ATTATCGTTA TTGTGCTGCT 
CTTTCTATGC TGGGGGAATA TGACTGGGCC CTGCAAGCAA ACATAAAAGC TCAAAAACTC 
TGTAAAAATG ACCCTGAGGG AATCAAGGAT CTAATTCAGC AGCATGTAAA GTTACAAAAA 
CAGATAGAAG ACCTGCAAGG TCGAACACCA GCTAGTAATC CAGTCAAAGC CTTCTGTGAA 
AGCAGGGCCT ACATACCTAG TTTATCAGCA CCTGCATTTA GTACTTCACT TAACTTTGTG 
GAAACTGAAA AAAATTTCAG AAAAACTAAC CACGAAATGG CAAACGGTGG TAATCAGAAT 
CCAAAGGTGG TAGATGAAGC TCTGAAAGTA GATGATTGTG ACTGTCATCC TGAATTTGTA 
CCACCATCAA GTCAGCCTCC AAAATATAAA GGGAAACAAA AAGCTCGAAA CAATGAATTG 
GAGAAGTTCA GCCCTGGTTC ACAAGAAACT TTACCAGTAG ATTTGAAAAA CATCTTGGAG 
AAACAGTTTT CTAAATCTTC CAGAGCTGCA CACCAGGATT TTGCTAATAT AATGAAAATG 
CTGAGAAGCT TAATCCAGGA TGGCTACACG GCCTTGCTGG AGCAGCGCTG CCGCAGTGCT 
GCCCAGGCCT TCACAGAGTT GCTCAACGGT TTAGATCCTC AAAAAATAAA GCAATTGAAT 
CTGGCCATGA TTAACTACGT GTTAGTAGTC TACGGACTTG CCATTTCACT CCTTGGAATA 
GGACGGCCTG AGGAGTTATC TGAAGCTGAA AACCAATTTA AGAGGATGAT TGAACACTAC 
CCCAATGAGG GACTTGATTG CTTGGCCTAC TGTGGAATTG GAAAAGTATA TTTGAAAAAA 
AACAGATTTT TAGAAGCTCT TAATCATTTT GAGAAAGCAA GAACCTTGAT CTGTCGTCTT 
CCTGGAGTGT TAACTTGGCC CACGAGTAAT GTGATTATTG AGGAGACTCA GCCAGGGAAG 
ATAAAGATGC TGTTAGAAAA ATTCGTTGAA GAATGCAGGT TCCCTCCAGT GCCGGATGCC 
ACTTGTTGCT ATCAGAAGTG CCATGGATAT TCCAAAATTC AGATATATAT AACCGATCCA 
GACTTCAAGG GTTTTATACG AATCAGCTGT TGCCAGTATT GTAAAATAGA ATTTCACATG 
AACTGCTGGA AGAAGTTAAA AACAACAACC TTCAATGATA AAATTGACAA GGTAAGACAA 
GATTTTCTGG AATTAATTTG TCTTACTCCT GATTGTAAAG GAATCATTTC AAAGATTATC 
ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG 
CTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA 
GAAGACAAAA AATTGAAACG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA 
AGACTGGAAG AGGACCTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT 
ATCAGCCGTG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG 
TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT 
TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG 
AATGAAGCAG TGGACTATGT TATTCGTCAT TTGATCCAGG AAAAGAACAG AGTAAAGACA 
AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG AGCCAAAACT AGCCACCTGG 
ATCCAGAAAC TTAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG 
GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATATGGT 
CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACGTCACTG 
AAGGAAGCGC GGTGTTTAAT ATGGCTGCTG GAAGAACACA GAGACAAGTT CCCGGCACTG 
CATAATGCCC TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT GTTAAGGAAA 
CAGGACAGTG GTGACGTGCC ATGTAATTTT ACCAAGATAA AAAACAAAGG CAAGAAAAAG 
AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC 
AATGAGACTT TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAATTC TGCGGGCCCC 
TTTGTAGTGC CTGATCACCT TCGGCAGGAT GTAGAAGAAT TTGAAGCTCT GTATGAACAG 
CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAAAAA 
TGTTCAACTC TATATGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG 
AGTAACAAGA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCG ACAGATACTA 
GAAAAAGCAG GAGGTTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAC 
AACTGTATTG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC 
ATTAAGACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA 
CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA 
CCTTTAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCATG CATCTGCTAA TTCTCCCCAA 
TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTCCGATA ATTCTTCCAG ATCAGTTTCT 
GAGGATGAGA AGCCCAAAGG GATATCTTCT GATTCTCCCA GGCCAGTCCT TGAGGAGACC 
ATTCACAAGC CAGTCTCCTC TAATTCTCCC AAACCAGTTC CCGAGGAAGT GAAAGCGACC 
TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGAGGATTT 
GACATTACCC AGACACCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC 
ATTTACACCC CCCTGGCCAG CGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG 
GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT 
GCTGAGAATG CCCATGGTAA CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG 
GAAACACCAG TGAAGTCAGA GTGCAGCTCT GATGGTGCTG ATATAGCCCA GAGTGAGGCT 
AGCGGAAACG AGGACTACTG TGGAGATTCT GCCAAGTGGG AAGTAAATCC AGAAAGCACC 
AATAAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA 
ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGAGGTATT 
TCACAGATTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT 
TATGAACACA GCAATGTCAG GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA 
AGGAACGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG 
AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA 
CTGAAGAAGA AAATGAAAAA GGTGTTGAAT GCCAGCAGAA TGTATACACA GGAAAACGAT 
GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT 
ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG 
AGTCTTCAGA GGGCTCTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGATGTGGAG 
GTGTGTAAGT TACAGAGCAT GGAGTCACAG GCCGAAGGGC TTCTCAAGAA CTTGAAGCTA 
ATGAGCAGTG ATTCGGCCGC ATATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG 
TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT 
CAGGCAATTA AAAATGGTTC TCGGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT 
TCGTTTCCTA CCTGTAACAC AATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAG 
GGTCAAGGTC CCTCCACTTC TGCAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG 
GAGTCAAGTC TGGGTTCTGC TGCTGACAGC CCAGTGGAGT CGCCCTCCTG CAGCCAGAAA 
GCCCCCCAGC TGCCAGAGCG TGAGGGCACT GGCCAGGCAG CTGGGCCAGA ACTAGGCCTG 
ATGGCTGATC AGAAGTTACC TGTTGCTCCA GAGCGTGCCA CTCGTCCAAG CCAGTCTCCA 
AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTCGGTGG TGTTCCCTTG TTACAGCAGC 
ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA 
TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACACAACACA TCCTTGATGA AGAGAGAAAG 
AAAAAGCCAA ATCCAGGAAA GGACCGGAGG CCGTCTGCAC CCAGCTCTGC TGTTCCTGCA 
CCCAGGGCCG CCCAGGACCC GCCCTCCGTG CCTGTCGGAG CATCACCCGA AACCAGGGGG 
CCGAAGACCG AAGACGTCCC TGTGTCCTGT GCCAATTCCT GTGAAATGTG CCAAGAAGCA 
TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA AGGGTGCTTC 
AAGCAGTGGT TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA 
GAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960471.1
CDS	5131..11139
Translation	MVPTVCYKGWCTMADFAQGSFSVADYALLEDCPYMDDCVFAPEFMSSDYVRVTQLYCDGVGRQYKDYTQTEGNLEFDICTMWCSKPVSVLQDYCDAIKVHIFWPLLFQRQQSSIITRMHSCVRTSGSHACEISLKKLQQLELMEDIVDLAKKVVNDTFFIGGLLRIGYKIENKILAMEEALNWVKSTGDVTILSKLGAVDNCWRMLSIFFTEYKYHITKIVTENCSLLEEFKTQSCAECLEQGELMKMKGNEEFSKERYDIAIIYYSRAIECRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKNNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPASNPVKAFCESRAYIPSLSAPAFSTSLNFVETEKNFRKTNHEMANGGNQNPKVVDEALKVDDCDCHPEFVPPSSQPPKYKGKQKARNNELEKFSPGSQETLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGRPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFVEECRFPPVPDATCCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKVRQDFLELICLTPDCKGIISKIIIFSSGGQVKCEFEHKVIKEKLPPRPILKQKCSSLEKLRLKEDKKLKRKLQKKEAKKLAQERLEEDLRESNPPKTEEQRETISRVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLTTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRVFLHVLSELKEVEPKLATWIQKLNSFGLDATGPFFSRYGAPLKELDFSIMTFLWNEKYGHKLGSIEGKQLDYFYEPTSLKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPCNFTKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETFTSGEDHIKRSLNSAGPFVVPDHLRQDVEEFEALYEQHSNEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKAFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPVSDLPLAPASEDMKPQHASANSPQSARDTGKPKPVSDNSSRSVSEDEKPKGISSDSPRPVLEETIHKPVSSNSPKPVPEEVKATYWPRPQLVTGYCTYLPFRGFDITQTPPAFISVLPTLPQYSIYTPLASVPTEYHLQRPIPVVPSFVASDITEVHFEAHHFSAENAHGNQIASETQILEESLETPVKSECSSDGADIAQSEASGNEDYCGDSAKWEVNPESTNKVTNIPCTQVVAVQVSWNITHQEVNTEPYGPFEAQQGGISQIEKEYQVLQEQLKEACENYEHSNVRGSEETKDLEEKLKRNVEENKISKTELDWFLEDLNKEIKKWEQEKKEIQERLKALKKKMKKVLNASRMYTQENDGKEKALELLLDQSLEISNTFTNEKMKIEESIKKGKEHYEESLQRALDAEVSVLENWKDVEVCKLQSMESQAEGLLKNLKLMSSDSAAYSDMDSDIHSWESFLSDVREEIEKAKSQFEEQIQAIKNGSRLSELPKVQISELSFPTCNTIHPKLLPESSGQEGQGPSTSASHGTGDQTAAPEESSLGSAADSPVESPSCSQKAPQLPEREGTGQAAGPELGLMADQKLPVAPERATRPSQSPKKPFNSIIEHLSVVFPCYSSTELAGFIKKVRNKNKTSLSGLSIDEIVQRVTQHILDEERKKKPNPGKDRRPSAPSSAVPAPRAAQDPPSVPVGASPETRGPKTEDVPVSCANSCEMCQEAFKSKHVRELQCGHRLHKGCFKQWLKSQRTCPACLGHDLLSEE

Target ORF information:

RefSeq Version	XM_012105081.2
Organism	Ovis aries(sheep)
Definition	Ovis aries tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012105081.2

ORF Insert Sequence:

ATGGTACCAA CGGTTTGCTA CAAAGGGTGG TGTACCATGG CCGATTTTGC CCAGGGAAGT 
TTTTCGGTAG CAGATTATGC CTTGTTAGAA GATTGCCCTT ACATGGACGA TTGTGTCTTT 
GCCCCTGAGT TTATGTCCAG TGATTATGTT CGTGTAACTC AGCTTTACTG TGATGGAGTG 
GGTAGACAGT ATAAAGATTA TACCCAAACT GAGGGAAACT TAGAATTCGA CATCTGCACT 
ATGTGGTGTA GTAAACCTGT TTCTGTCCTG CAAGATTACT GTGATGCCAT TAAAGTCCAC 
ATCTTCTGGC CACTTCTGTT TCAACGTCAG CAGAGCTCCA TAATAACACG GATGCACTCC 
TGTGTAAGGA CCAGCGGTTC TCATGCTTGT GAGATAAGTT TGAAGAAATT ACAGCAGCTT 
GAGCTGATGG AAGATATTGT GGATTTGGCA AAGAAAGTTG TGAATGATAC ATTCTTTATC 
GGAGGCTTAT TGAGAATTGG CTATAAAATA GAGAATAAAA TCTTGGCAAT GGAAGAAGCT 
TTGAATTGGG TGAAATCCAC AGGCGATGTA ACAATTTTAT CTAAATTAGG AGCAGTTGAC 
AATTGTTGGC GTATGTTAAG TATTTTCTTT ACTGAATACA AGTATCACAT AACTAAAATT 
GTAACAGAAA ACTGCAGTTT GCTTGAAGAA TTTAAAACTC AAAGTTGTGC GGAGTGTCTA 
GAGCAAGGAG AACTAATGAA AATGAAAGGA AATGAAGAGT TTTCCAAAGA AAGATATGAT 
ATAGCTATTA TCTATTACTC CAGAGCCATT GAATGTAGGC CTGAAAATCA CCTGCTTTAC 
GGTAACCGAG CTCTTTGTTT TCTTCGTACT GGACAATTCA GAAATGCACT TGGTGATGGA 
AAGAGAGCTA CCATTCTGAA GAACAATTGG ACAAAGGGTC ATTATCGTTA TTGTGCTGCT 
CTTTCTATGC TGGGGGAATA TGACTGGGCC CTGCAAGCAA ACATAAAAGC TCAAAAACTC 
TGTAAAAATG ACCCTGAGGG AATCAAGGAT CTAATTCAGC AGCATGTAAA GTTACAAAAA 
CAGATAGAAG ACCTGCAAGG TCGAACACCA GCTAGTAATC CAGTCAAAGC CTTCTGTGAA 
AGCAGGGCCT ACATACCTAG TTTATCAGCA CCTGCATTTA GTACTTCACT TAACTTTGTG 
GAAACTGAAA AAAATTTCAG AAAAACTAAC CACGAAATGG CAAACGGTGG TAATCAGAAT 
CCAAAGGTGG TAGATGAAGC TCTGAAAGTA GATGATTGTG ACTGTCATCC TGAATTTGTA 
CCACCATCAA GTCAGCCTCC AAAATATAAA GGGAAACAAA AAGCTCGAAA CAATGAATTG 
GAGAAGTTCA GCCCTGGTTC ACAAGAAACT TTACCAGTAG ATTTGAAAAA CATCTTGGAG 
AAACAGTTTT CTAAATCTTC CAGAGCTGCA CACCAGGATT TTGCTAATAT AATGAAAATG 
CTGAGAAGCT TAATCCAGGA TGGCTACACG GCCTTGCTGG AGCAGCGCTG CCGCAGTGCT 
GCCCAGGCCT TCACAGAGTT GCTCAACGGT TTAGATCCTC AAAAAATAAA GCAATTGAAT 
CTGGCCATGA TTAACTACGT GTTAGTAGTC TACGGACTTG CCATTTCACT CCTTGGAATA 
GGACGGCCTG AGGAGTTATC TGAAGCTGAA AACCAATTTA AGAGGATGAT TGAACACTAC 
CCCAATGAGG GACTTGATTG CTTGGCCTAC TGTGGAATTG GAAAAGTATA TTTGAAAAAA 
AACAGATTTT TAGAAGCTCT TAATCATTTT GAGAAAGCAA GAACCTTGAT CTGTCGTCTT 
CCTGGAGTGT TAACTTGGCC CACGAGTAAT GTGATTATTG AGGAGACTCA GCCAGGGAAG 
ATAAAGATGC TGTTAGAAAA ATTCGTTGAA GAATGCAGGT TCCCTCCAGT GCCGGATGCC 
ACTTGTTGCT ATCAGAAGTG CCATGGATAT TCCAAAATTC AGATATATAT AACCGATCCA 
GACTTCAAGG GTTTTATACG AATCAGCTGT TGCCAGTATT GTAAAATAGA ATTTCACATG 
AACTGCTGGA AGAAGTTAAA AACAACAACC TTCAATGATA AAATTGACAA GGTAAGACAA 
GATTTTCTGG AATTAATTTG TCTTACTCCT GATTGTAAAG GAATCATTTC AAAGATTATC 
ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG 
CTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA 
GAAGACAAAA AATTGAAACG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA 
AGACTGGAAG AGGACCTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT 
ATCAGCCGTG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG 
TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT 
TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG 
AATGAAGCAG TGGACTATGT TATTCGTCAT TTGATCCAGG AAAAGAACAG AGTAAAGACA 
AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG AGCCAAAACT AGCCACCTGG 
ATCCAGAAAC TTAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG 
GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATATGGT 
CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACGTCACTG 
AAGGAAGCGC GGTGTTTAAT ATGGCTGCTG GAAGAACACA GAGACAAGTT CCCGGCACTG 
CATAATGCCC TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT GTTAAGGAAA 
CAGGACAGTG GTGACGTGCC ATGTAATTTT ACCAAGATAA AAAACAAAGG CAAGAAAAAG 
AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC 
AATGAGACTT TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAATTC TGCGGGCCCC 
TTTGTAGTGC CTGATCACCT TCGGCAGGAT GTAGAAGAAT TTGAAGCTCT GTATGAACAG 
CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAAAAA 
TGTTCAACTC TATATGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG 
AGTAACAAGA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCG ACAGATACTA 
GAAAAAGCAG GAGGTTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAC 
AACTGTATTG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC 
ATTAAGACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA 
CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA 
CCTTTAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCATG CATCTGCTAA TTCTCCCCAA 
TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTCCGATA ATTCTTCCAG ATCAGTTTCT 
GAGGATGAGA AGCCCAAAGG GATATCTTCT GATTCTCCCA GGCCAGTCCT TGAGGAGACC 
ATTCACAAGC CAGTCTCCTC TAATTCTCCC AAACCAGTTC CCGAGGAAGT GAAAGCGACC 
TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGAGGATTT 
GACATTACCC AGACACCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC 
ATTTACACCC CCCTGGCCAG CGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG 
GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT 
GCTGAGAATG CCCATGGTAA CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG 
GAAACACCAG TGAAGTCAGA GTGCAGCTCT GATGGTGCTG ATATAGCCCA GAGTGAGGCT 
AGCGGAAACG AGGACTACTG TGGAGATTCT GCCAAGTGGG AAGTAAATCC AGAAAGCACC 
AATAAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA 
ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGAGGTATT 
TCACAGATTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT 
TATGAACACA GCAATGTCAG GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA 
AGGAACGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG 
AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA 
CTGAAGAAGA AAATGAAAAA GGTGTTGAAT GCCAGCAGAA TGTATACACA GGAAAACGAT 
GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT 
ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG 
AGTCTTCAGA GGGCTCTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGATGTGGAG 
GTGTGTAAGT TACAGAGCAT GGAGTCACAG GCCGAAGGGC TTCTCAAGAA CTTGAAGCTA 
ATGAGCAGTG ATTCGGCCGC ATATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG 
TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT 
CAGGCAATTA AAAATGGTTC TCGGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT 
TCGTTTCCTA CCTGTAACAC AATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAG 
GGTCAAGGTC CCTCCACTTC TGCAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG 
GAGTCAAGTC TGGGTTCTGC TGCTGACAGC CCAGTGGAGT CGCCCTCCTG CAGCCAGAAA 
GCCCCCCAGC TGCCAGAGCG TGAGGGCACT GGCCAGGCAG CTGGGCCAGA ACTAGGCCTG 
ATGGCTGATC AGAAGTTACC TGTTGCTCCA GAGCGTGCCA CTCGTCCAAG CCAGTCTCCA 
AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTCGGTGG TGTTCCCTTG TTACAGCAGC 
ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA 
TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACACAACACA TCCTTGATGA AGAGAGAAAG 
AAAAAGCCAA ATCCAGGAAA GGACCGGAGG CCGTCTGCAC CCAGCTCTGC TGTTCCTGCA 
CCCAGGGCCG CCCAGGACCC GCCCTCCGTG CCTGTCGGAG CATCACCCGA AACCAGGGGG 
CCGAAGACCG AAGACGTCCC TGTGTCCTGT GCCAATTCCT GTGAAATGTG CCAAGAAGCA 
TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA AGGGTGCTTC 
AAGCAGTGGT TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA 
GAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TTC3
Entrez Gene ID	101116444
Full Name	tetratricopeptide repeat domain 3
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TTC3 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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