HLCS gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_012105018.2	XP_011960408.1	biotin--protein ligase isoform X5
XM_012130637.2	XP_011986027.1	biotin--protein ligase isoform X3
XM_027961598.1	XP_027817399.1	biotin--protein ligase isoform X3
XM_012104991.2	XP_011960381.1	biotin--protein ligase isoform X1
XM_012105038.2	XP_011960428.1	biotin--protein ligase isoform X5
XM_012105047.2	XP_011960437.1	biotin--protein ligase isoform X6
XM_012130629.2	XP_011986019.1	biotin--protein ligase isoform X1
XM_027961593.1	XP_027817394.1	biotin--protein ligase isoform X1
XM_012130631.2	XP_011986021.1	biotin--protein ligase isoform X2
XM_012130632.2	XP_011986022.1	biotin--protein ligase isoform X4
XM_012105003.2	XP_011960393.1	biotin--protein ligase isoform X4
XM_012105031.2	XP_011960421.1	biotin--protein ligase isoform X5
XM_012104997.3	XP_011960387.2	biotin--protein ligase isoform X2
XM_012104992.2	XP_011960382.1	biotin--protein ligase isoform X2
XM_012130630.2	XP_011986020.1	biotin--protein ligase isoform X7
XM_012130636.2	XP_011986026.1	biotin--protein ligase isoform X5
XM_012130635.2	XP_011986025.1	biotin--protein ligase isoform X5
XM_012104997.2	XP_011960387.1	biotin--protein ligase isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm30534	XM_012104991.2 Latest version!	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OSm30535	XM_012104992.2 Latest version!	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSm30536	XM_012104997.2 Latest version!	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSm30537	XM_012105003.2 Latest version!	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSm30538	XM_012105031.2 Latest version!	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSm30538	XM_012105018.2 Latest version!	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSm30538	XM_012105038.2 Latest version!	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSm30539	XM_012105047.2 Latest version!	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OOv13969	XM_012130629.2 Latest version!	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOv13971	XM_012130631.2 Latest version!	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OOv13972	XM_012130637.2 Latest version!	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OOv13973	XM_012130632.2 Latest version!	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OOv13974	XM_012130636.2 Latest version!	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OOv13974	XM_012130635.2 Latest version!	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OSm59209	XM_027961593.1 Latest version!	Ovis aries holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSm59210	XM_012104997.3 Latest version!	Ovis aries holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSm59211	XM_027961598.1 Latest version!	Ovis aries holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OOv13970	XM_012130630.2 Latest version!	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

HLCS ( XM_012104991.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960381.1 Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm30534
Clone ID Related Accession (Same CDS sequence)	XM_012104991.2
Accession Version	XM_012104991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012104991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAAAG GGGGAGGGAG GAGGGCAGCG GGAACGGTGC TCCGGAGCGG CCAGAAGCCC 
CGGCCAATGT CAGCGGAGGC TGGAGCAGTG GGCAGGATTT GGGGTTGTGC CGGGCAGCGG 
GGCCCCCACC AAGGGGGATC TCCACCCGGC CTTTCCCACG GCCTGCTTCC CACTCCCCCG 
CGGCCACCTG CTCTGTCCTT GGGGGAACGC TTCTGCCCGC CCCCCACCCC CCTCCCAAAT 
CAGGCGCACT GGCCGCGGGG TCCCGGGGCT TGGCCCACAG GTCCCTGTTA CTGGGGGCTC 
CCTCTTAACC AGTCCATTGA TGACTTAAAC AAATGGGCCC TGTTCCTGGT TTCTCCTTTT 
ATACTTGAAG CAGAGCACAT CGCCTTTGTG ACAGAGAGCA TTTGGGTGCA AGGTGAGAAT 
TTACAGAGAC CAGCATCCTC TTCGGAAACT ATTGTGAAGT GGTCTGACTG CTGTTTGCCG 
TTAGCTTGCC GACCTGGGGA TCCTTATCAA TTAATCGCCA AAGCAAGTGT GGATGACTTC 
AGCAAGCTAG GGGTGGCATT CCTGGAAGAT AGACTCCAGA TGGCTAATGG ACTGATACCT 
CAGAAGATCG TGTCTGTCCA CTTGCAGGAC TCTGCCCTAA AGGAACTTAA GGGTCAGGCC 
TCAGATCCCC CAGCCCAGCT CCTGCAGCCC GACCCCGAAT CTTATATTCA GAGGAACCCT 
CCTGAGCCCG ACACTATGGA GGACCCCCAG TCTCCGGGTT CTGCATCCAC TCGGGGGCCA 
GGTGGGGCCC CTAGGGGCGG TGAGCCAGCC GTGGACGGTG ACAGAGGCGT GGGCTCTGTC 
GAGCACTGTC CCCTCCATCT GTCCAGCTGC CACGAGTGTT TGGAGCTTGA GAACAGCACC 
ATTGAATCCG TCAGGTTTGC CTCTGCAGAG AACATTCCAG ATCTCCCGTA CGACTACAGC 
GGCAGTTTGG AGGGCCTCGC TGACGACCTC CCCACCGAGA GGGATGGGAG GAGGGTCAAC 
GTCACGGGCA AGGCCCCCAA CATCCTCCTG TATGTGGGCT CTGACTCCCA GGATGGCCAG 
GACAGGCTCC AGCAGGTCCG ATCGGCGCTG GCTGACTGTG TGGACACGGA CTGTTACACT 
CTGTACCACC TGCCTCCGGA AAGCGCTCTC AGGGACCCGT GGCCGGACAA CTGTCTGCTG 
CTGGTCATCG CCACCGGGGA GCCCGTCCCC GAGGACCTGT CCCGGAGGTT CACGGCCTAT 
CTTTCTCAGG GAGGGAAGGT GCTGGCCCTG GCTTCGTCCT TCACGCTGCC TGGCTTCCAG 
GTGACCAGCA AGGCCCCGCT GCAGGACACG GTCCAGAGCT TGGTTTTCTG CAAGGTGGAT 
GGGAGCCTGG TGAGGCTTAG CATCCTGAGC AGTGGCTACG TTTACGAGGA GGGGCCTGGG 
GAGCCGCTCG GCCCCAGCAA GCTCCACGGC CACCTGGACA GTGAGGACGG GGACAGGCTG 
GTGGTGCAAG TGCCTTTCGG GACTCATGGA GGAGAAGCTG TTCTCTGCCA GGTGCACTTG 
GAACTACCTC CCAGCTCTCC AGCAGTGCAA ACACAAGAGG ATTTTAACCT GCTCAAATCA 
AGCAACTTTA GAAGATATGA AGTCCTTACG GAGATCCTGA CGACACTGGG CCTAAGCTGC 
GACAGAAAGC AAGCTCCTGC CTTAACGCCT CTTTACCTGC TGTCGGCCAC GGAGGAAATC 
CGGGATCCTC TTATGCAGTG GCTGCGGAAA CATGTGGATC CTGAAGGAGT AATAAGATCC 
AGCAAGCTCT CCCTGAAATT CATTTCATCC TACACGTCTG AAATAGAAAT CACCCCATCC 
ACCATACCCG TGGTGACTGA CACGGAGTCC TTCTCATCAG AAAACTTTGA CTTCGAGATC 
TACCGACAAA ACCTGAAGAC AAGGAAACTT GGAAAAGTCA TTCTGTTTGC CGAAGTGACG 
CCCACCACAA TGAGTCTTCT GGACGGGTTG ATGTTTGAGA TGCCGCAGGA AATGGGTTTA 
ATAGCCATCG CAGTCCGCCA GACGCAGGGC AAAGGGCGGG GAAAGAATGC TTGGCTGAGC 
CCCTTGGGAT GCGCCCTTTC CACCCTGCTG GTCTGCATCC CGCTGAGATC CCCGCTGGGA 
CAGAGGATTC CGTTTGTCCA GCATCTGATG TCCCTGGCCG TCGTAGAGGC AGTAAGATCC 
ATCCCCGGGT ACCAGGATAT CAATTTGCGA GTGAAGTGGC CCAATGATAT TTATTACAGT 
GACCTCATGA AGCTTGGTGG AGTTTTGGTT AACTCCACAC TTATGGGAGA AACATTTTAT 
ATACTTATTG GCTGTGGATT TAACGTGACG AACAGCAACC CCACCATATG CATCAATGAT 
CTCATCACAG AATACAATAA GGAACACGGG GCAGAGCTAC AGCCCTTGAG AGCTGACTGT 
CTCATCGCCC GAACGGCGAC CGTGCTGGAG AAGCTGATCG ACACGTTTCA GGACCAGGGG 
CCCAACGGCG TTCTTCCCCT CTACTATAAA TACTGGGTAC ACAGTGCTCA GCAAGTCCAC 
CTGGGAAGCG CTGAGGGACC AAAGGTGTGG ATAGTTGGCC TGGATGACTC CGGGTTCCTT 
CTGGTCCACC AGGAAAACGG CGATGTGGTC ACGGTGCATC CTGACGGCAA CTCCTTCGAC 
ATGCTGAGAA ACCTCATTGT CCCCAAACAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960381.1
CDS	3387..6122
Translation	MPKGGGRRAAGTVLRSGQKPRPMSAEAGAVGRIWGCAGQRGPHQGGSPPGLSHGLLPTPPRPPALSLGERFCPPPTPLPNQAHWPRGPGAWPTGPCYWGLPLNQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012104991.2
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012104991.2

ORF Insert Sequence:

ATGCCAAAAG GGGGAGGGAG GAGGGCAGCG GGAACGGTGC TCCGGAGCGG CCAGAAGCCC 
CGGCCAATGT CAGCGGAGGC TGGAGCAGTG GGCAGGATTT GGGGTTGTGC CGGGCAGCGG 
GGCCCCCACC AAGGGGGATC TCCACCCGGC CTTTCCCACG GCCTGCTTCC CACTCCCCCG 
CGGCCACCTG CTCTGTCCTT GGGGGAACGC TTCTGCCCGC CCCCCACCCC CCTCCCAAAT 
CAGGCGCACT GGCCGCGGGG TCCCGGGGCT TGGCCCACAG GTCCCTGTTA CTGGGGGCTC 
CCTCTTAACC AGTCCATTGA TGACTTAAAC AAATGGGCCC TGTTCCTGGT TTCTCCTTTT 
ATACTTGAAG CAGAGCACAT CGCCTTTGTG ACAGAGAGCA TTTGGGTGCA AGGTGAGAAT 
TTACAGAGAC CAGCATCCTC TTCGGAAACT ATTGTGAAGT GGTCTGACTG CTGTTTGCCG 
TTAGCTTGCC GACCTGGGGA TCCTTATCAA TTAATCGCCA AAGCAAGTGT GGATGACTTC 
AGCAAGCTAG GGGTGGCATT CCTGGAAGAT AGACTCCAGA TGGCTAATGG ACTGATACCT 
CAGAAGATCG TGTCTGTCCA CTTGCAGGAC TCTGCCCTAA AGGAACTTAA GGGTCAGGCC 
TCAGATCCCC CAGCCCAGCT CCTGCAGCCC GACCCCGAAT CTTATATTCA GAGGAACCCT 
CCTGAGCCCG ACACTATGGA GGACCCCCAG TCTCCGGGTT CTGCATCCAC TCGGGGGCCA 
GGTGGGGCCC CTAGGGGCGG TGAGCCAGCC GTGGACGGTG ACAGAGGCGT GGGCTCTGTC 
GAGCACTGTC CCCTCCATCT GTCCAGCTGC CACGAGTGTT TGGAGCTTGA GAACAGCACC 
ATTGAATCCG TCAGGTTTGC CTCTGCAGAG AACATTCCAG ATCTCCCGTA CGACTACAGC 
GGCAGTTTGG AGGGCCTCGC TGACGACCTC CCCACCGAGA GGGATGGGAG GAGGGTCAAC 
GTCACGGGCA AGGCCCCCAA CATCCTCCTG TATGTGGGCT CTGACTCCCA GGATGGCCAG 
GACAGGCTCC AGCAGGTCCG ATCGGCGCTG GCTGACTGTG TGGACACGGA CTGTTACACT 
CTGTACCACC TGCCTCCGGA AAGCGCTCTC AGGGACCCGT GGCCGGACAA CTGTCTGCTG 
CTGGTCATCG CCACCGGGGA GCCCGTCCCC GAGGACCTGT CCCGGAGGTT CACGGCCTAT 
CTTTCTCAGG GAGGGAAGGT GCTGGCCCTG GCTTCGTCCT TCACGCTGCC TGGCTTCCAG 
GTGACCAGCA AGGCCCCGCT GCAGGACACG GTCCAGAGCT TGGTTTTCTG CAAGGTGGAT 
GGGAGCCTGG TGAGGCTTAG CATCCTGAGC AGTGGCTACG TTTACGAGGA GGGGCCTGGG 
GAGCCGCTCG GCCCCAGCAA GCTCCACGGC CACCTGGACA GTGAGGACGG GGACAGGCTG 
GTGGTGCAAG TGCCTTTCGG GACTCATGGA GGAGAAGCTG TTCTCTGCCA GGTGCACTTG 
GAACTACCTC CCAGCTCTCC AGCAGTGCAA ACACAAGAGG ATTTTAACCT GCTCAAATCA 
AGCAACTTTA GAAGATATGA AGTCCTTACG GAGATCCTGA CGACACTGGG CCTAAGCTGC 
GACAGAAAGC AAGCTCCTGC CTTAACGCCT CTTTACCTGC TGTCGGCCAC GGAGGAAATC 
CGGGATCCTC TTATGCAGTG GCTGCGGAAA CATGTGGATC CTGAAGGAGT AATAAGATCC 
AGCAAGCTCT CCCTGAAATT CATTTCATCC TACACGTCTG AAATAGAAAT CACCCCATCC 
ACCATACCCG TGGTGACTGA CACGGAGTCC TTCTCATCAG AAAACTTTGA CTTCGAGATC 
TACCGACAAA ACCTGAAGAC AAGGAAACTT GGAAAAGTCA TTCTGTTTGC CGAAGTGACG 
CCCACCACAA TGAGTCTTCT GGACGGGTTG ATGTTTGAGA TGCCGCAGGA AATGGGTTTA 
ATAGCCATCG CAGTCCGCCA GACGCAGGGC AAAGGGCGGG GAAAGAATGC TTGGCTGAGC 
CCCTTGGGAT GCGCCCTTTC CACCCTGCTG GTCTGCATCC CGCTGAGATC CCCGCTGGGA 
CAGAGGATTC CGTTTGTCCA GCATCTGATG TCCCTGGCCG TCGTAGAGGC AGTAAGATCC 
ATCCCCGGGT ACCAGGATAT CAATTTGCGA GTGAAGTGGC CCAATGATAT TTATTACAGT 
GACCTCATGA AGCTTGGTGG AGTTTTGGTT AACTCCACAC TTATGGGAGA AACATTTTAT 
ATACTTATTG GCTGTGGATT TAACGTGACG AACAGCAACC CCACCATATG CATCAATGAT 
CTCATCACAG AATACAATAA GGAACACGGG GCAGAGCTAC AGCCCTTGAG AGCTGACTGT 
CTCATCGCCC GAACGGCGAC CGTGCTGGAG AAGCTGATCG ACACGTTTCA GGACCAGGGG 
CCCAACGGCG TTCTTCCCCT CTACTATAAA TACTGGGTAC ACAGTGCTCA GCAAGTCCAC 
CTGGGAAGCG CTGAGGGACC AAAGGTGTGG ATAGTTGGCC TGGATGACTC CGGGTTCCTT 
CTGGTCCACC AGGAAAACGG CGATGTGGTC ACGGTGCATC CTGACGGCAA CTCCTTCGAC 
ATGCTGAGAA ACCTCATTGT CCCCAAACAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012104992.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960382.1 Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSm30535
Clone ID Related Accession (Same CDS sequence)	XM_012104992.2
Accession Version	XM_012104992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012104992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATC TTCAGTCTTC ATGGCGGCAG TCCATTGATG ACTTAAACAA ATGGGCCCTG 
TTCCTGGTTT CTCCTTTTAT ACTTGAAGCA GAGCACATCG CCTTTGTGAC AGAGAGCATT 
TGGGTGCAAG GTGAGAATTT ACAGAGACCA GCATCCTCTT CGGAAACTAT TGTGAAGTGG 
TCTGACTGCT GTTTGCCGTT AGCTTGCCGA CCTGGGGATC CTTATCAATT AATCGCCAAA 
GCAAGTGTGG ATGACTTCAG CAAGCTAGGG GTGGCATTCC TGGAAGATAG ACTCCAGATG 
GCTAATGGAC TGATACCTCA GAAGATCGTG TCTGTCCACT TGCAGGACTC TGCCCTAAAG 
GAACTTAAGG GTCAGGCCTC AGATCCCCCA GCCCAGCTCC TGCAGCCCGA CCCCGAATCT 
TATATTCAGA GGAACCCTCC TGAGCCCGAC ACTATGGAGG ACCCCCAGTC TCCGGGTTCT 
GCATCCACTC GGGGGCCAGG TGGGGCCCCT AGGGGCGGTG AGCCAGCCGT GGACGGTGAC 
AGAGGCGTGG GCTCTGTCGA GCACTGTCCC CTCCATCTGT CCAGCTGCCA CGAGTGTTTG 
GAGCTTGAGA ACAGCACCAT TGAATCCGTC AGGTTTGCCT CTGCAGAGAA CATTCCAGAT 
CTCCCGTACG ACTACAGCGG CAGTTTGGAG GGCCTCGCTG ACGACCTCCC CACCGAGAGG 
GATGGGAGGA GGGTCAACGT CACGGGCAAG GCCCCCAACA TCCTCCTGTA TGTGGGCTCT 
GACTCCCAGG ATGGCCAGGA CAGGCTCCAG CAGGTCCGAT CGGCGCTGGC TGACTGTGTG 
GACACGGACT GTTACACTCT GTACCACCTG CCTCCGGAAA GCGCTCTCAG GGACCCGTGG 
CCGGACAACT GTCTGCTGCT GGTCATCGCC ACCGGGGAGC CCGTCCCCGA GGACCTGTCC 
CGGAGGTTCA CGGCCTATCT TTCTCAGGGA GGGAAGGTGC TGGCCCTGGC TTCGTCCTTC 
ACGCTGCCTG GCTTCCAGGT GACCAGCAAG GCCCCGCTGC AGGACACGGT CCAGAGCTTG 
GTTTTCTGCA AGGTGGATGG GAGCCTGGTG AGGCTTAGCA TCCTGAGCAG TGGCTACGTT 
TACGAGGAGG GGCCTGGGGA GCCGCTCGGC CCCAGCAAGC TCCACGGCCA CCTGGACAGT 
GAGGACGGGG ACAGGCTGGT GGTGCAAGTG CCTTTCGGGA CTCATGGAGG AGAAGCTGTT 
CTCTGCCAGG TGCACTTGGA ACTACCTCCC AGCTCTCCAG CAGTGCAAAC ACAAGAGGAT 
TTTAACCTGC TCAAATCAAG CAACTTTAGA AGATATGAAG TCCTTACGGA GATCCTGACG 
ACACTGGGCC TAAGCTGCGA CAGAAAGCAA GCTCCTGCCT TAACGCCTCT TTACCTGCTG 
TCGGCCACGG AGGAAATCCG GGATCCTCTT ATGCAGTGGC TGCGGAAACA TGTGGATCCT 
GAAGGAGTAA TAAGATCCAG CAAGCTCTCC CTGAAATTCA TTTCATCCTA CACGTCTGAA 
ATAGAAATCA CCCCATCCAC CATACCCGTG GTGACTGACA CGGAGTCCTT CTCATCAGAA 
AACTTTGACT TCGAGATCTA CCGACAAAAC CTGAAGACAA GGAAACTTGG AAAAGTCATT 
CTGTTTGCCG AAGTGACGCC CACCACAATG AGTCTTCTGG ACGGGTTGAT GTTTGAGATG 
CCGCAGGAAA TGGGTTTAAT AGCCATCGCA GTCCGCCAGA CGCAGGGCAA AGGGCGGGGA 
AAGAATGCTT GGCTGAGCCC CTTGGGATGC GCCCTTTCCA CCCTGCTGGT CTGCATCCCG 
CTGAGATCCC CGCTGGGACA GAGGATTCCG TTTGTCCAGC ATCTGATGTC CCTGGCCGTC 
GTAGAGGCAG TAAGATCCAT CCCCGGGTAC CAGGATATCA ATTTGCGAGT GAAGTGGCCC 
AATGATATTT ATTACAGTGA CCTCATGAAG CTTGGTGGAG TTTTGGTTAA CTCCACACTT 
ATGGGAGAAA CATTTTATAT ACTTATTGGC TGTGGATTTA ACGTGACGAA CAGCAACCCC 
ACCATATGCA TCAATGATCT CATCACAGAA TACAATAAGG AACACGGGGC AGAGCTACAG 
CCCTTGAGAG CTGACTGTCT CATCGCCCGA ACGGCGACCG TGCTGGAGAA GCTGATCGAC 
ACGTTTCAGG ACCAGGGGCC CAACGGCGTT CTTCCCCTCT ACTATAAATA CTGGGTACAC 
AGTGCTCAGC AAGTCCACCT GGGAAGCGCT GAGGGACCAA AGGTGTGGAT AGTTGGCCTG 
GATGACTCCG GGTTCCTTCT GGTCCACCAG GAAAACGGCG ATGTGGTCAC GGTGCATCCT 
GACGGCAACT CCTTCGACAT GCTGAGAAAC CTCATTGTCC CCAAACAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960382.1
CDS	155..2608
Translation	MQDLQSSWRQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012104992.2
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012104992.2

ORF Insert Sequence:

ATGCAGGATC TTCAGTCTTC ATGGCGGCAG TCCATTGATG ACTTAAACAA ATGGGCCCTG 
TTCCTGGTTT CTCCTTTTAT ACTTGAAGCA GAGCACATCG CCTTTGTGAC AGAGAGCATT 
TGGGTGCAAG GTGAGAATTT ACAGAGACCA GCATCCTCTT CGGAAACTAT TGTGAAGTGG 
TCTGACTGCT GTTTGCCGTT AGCTTGCCGA CCTGGGGATC CTTATCAATT AATCGCCAAA 
GCAAGTGTGG ATGACTTCAG CAAGCTAGGG GTGGCATTCC TGGAAGATAG ACTCCAGATG 
GCTAATGGAC TGATACCTCA GAAGATCGTG TCTGTCCACT TGCAGGACTC TGCCCTAAAG 
GAACTTAAGG GTCAGGCCTC AGATCCCCCA GCCCAGCTCC TGCAGCCCGA CCCCGAATCT 
TATATTCAGA GGAACCCTCC TGAGCCCGAC ACTATGGAGG ACCCCCAGTC TCCGGGTTCT 
GCATCCACTC GGGGGCCAGG TGGGGCCCCT AGGGGCGGTG AGCCAGCCGT GGACGGTGAC 
AGAGGCGTGG GCTCTGTCGA GCACTGTCCC CTCCATCTGT CCAGCTGCCA CGAGTGTTTG 
GAGCTTGAGA ACAGCACCAT TGAATCCGTC AGGTTTGCCT CTGCAGAGAA CATTCCAGAT 
CTCCCGTACG ACTACAGCGG CAGTTTGGAG GGCCTCGCTG ACGACCTCCC CACCGAGAGG 
GATGGGAGGA GGGTCAACGT CACGGGCAAG GCCCCCAACA TCCTCCTGTA TGTGGGCTCT 
GACTCCCAGG ATGGCCAGGA CAGGCTCCAG CAGGTCCGAT CGGCGCTGGC TGACTGTGTG 
GACACGGACT GTTACACTCT GTACCACCTG CCTCCGGAAA GCGCTCTCAG GGACCCGTGG 
CCGGACAACT GTCTGCTGCT GGTCATCGCC ACCGGGGAGC CCGTCCCCGA GGACCTGTCC 
CGGAGGTTCA CGGCCTATCT TTCTCAGGGA GGGAAGGTGC TGGCCCTGGC TTCGTCCTTC 
ACGCTGCCTG GCTTCCAGGT GACCAGCAAG GCCCCGCTGC AGGACACGGT CCAGAGCTTG 
GTTTTCTGCA AGGTGGATGG GAGCCTGGTG AGGCTTAGCA TCCTGAGCAG TGGCTACGTT 
TACGAGGAGG GGCCTGGGGA GCCGCTCGGC CCCAGCAAGC TCCACGGCCA CCTGGACAGT 
GAGGACGGGG ACAGGCTGGT GGTGCAAGTG CCTTTCGGGA CTCATGGAGG AGAAGCTGTT 
CTCTGCCAGG TGCACTTGGA ACTACCTCCC AGCTCTCCAG CAGTGCAAAC ACAAGAGGAT 
TTTAACCTGC TCAAATCAAG CAACTTTAGA AGATATGAAG TCCTTACGGA GATCCTGACG 
ACACTGGGCC TAAGCTGCGA CAGAAAGCAA GCTCCTGCCT TAACGCCTCT TTACCTGCTG 
TCGGCCACGG AGGAAATCCG GGATCCTCTT ATGCAGTGGC TGCGGAAACA TGTGGATCCT 
GAAGGAGTAA TAAGATCCAG CAAGCTCTCC CTGAAATTCA TTTCATCCTA CACGTCTGAA 
ATAGAAATCA CCCCATCCAC CATACCCGTG GTGACTGACA CGGAGTCCTT CTCATCAGAA 
AACTTTGACT TCGAGATCTA CCGACAAAAC CTGAAGACAA GGAAACTTGG AAAAGTCATT 
CTGTTTGCCG AAGTGACGCC CACCACAATG AGTCTTCTGG ACGGGTTGAT GTTTGAGATG 
CCGCAGGAAA TGGGTTTAAT AGCCATCGCA GTCCGCCAGA CGCAGGGCAA AGGGCGGGGA 
AAGAATGCTT GGCTGAGCCC CTTGGGATGC GCCCTTTCCA CCCTGCTGGT CTGCATCCCG 
CTGAGATCCC CGCTGGGACA GAGGATTCCG TTTGTCCAGC ATCTGATGTC CCTGGCCGTC 
GTAGAGGCAG TAAGATCCAT CCCCGGGTAC CAGGATATCA ATTTGCGAGT GAAGTGGCCC 
AATGATATTT ATTACAGTGA CCTCATGAAG CTTGGTGGAG TTTTGGTTAA CTCCACACTT 
ATGGGAGAAA CATTTTATAT ACTTATTGGC TGTGGATTTA ACGTGACGAA CAGCAACCCC 
ACCATATGCA TCAATGATCT CATCACAGAA TACAATAAGG AACACGGGGC AGAGCTACAG 
CCCTTGAGAG CTGACTGTCT CATCGCCCGA ACGGCGACCG TGCTGGAGAA GCTGATCGAC 
ACGTTTCAGG ACCAGGGGCC CAACGGCGTT CTTCCCCTCT ACTATAAATA CTGGGTACAC 
AGTGCTCAGC AAGTCCACCT GGGAAGCGCT GAGGGACCAA AGGTGTGGAT AGTTGGCCTG 
GATGACTCCG GGTTCCTTCT GGTCCACCAG GAAAACGGCG ATGTGGTCAC GGTGCATCCT 
GACGGCAACT CCTTCGACAT GCTGAGAAAC CTCATTGTCC CCAAACAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012104997.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960387.1 Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSm30536
Clone ID Related Accession (Same CDS sequence)	XM_012104997.2
Accession Version	XM_012104997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012104997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGG ATCCAGATGC AAGGTCCATT GATGACTTAA ACAAATGGGC CCTGTTCCTG 
GTTTCTCCTT TTATACTTGA AGCAGAGCAC ATCGCCTTTG TGACAGAGAG CATTTGGGTG 
CAAGGTGAGA ATTTACAGAG ACCAGCATCC TCTTCGGAAA CTATTGTGAA GTGGTCTGAC 
TGCTGTTTGC CGTTAGCTTG CCGACCTGGG GATCCTTATC AATTAATCGC CAAAGCAAGT 
GTGGATGACT TCAGCAAGCT AGGGGTGGCA TTCCTGGAAG ATAGACTCCA GATGGCTAAT 
GGACTGATAC CTCAGAAGAT CGTGTCTGTC CACTTGCAGG ACTCTGCCCT AAAGGAACTT 
AAGGGTCAGG CCTCAGATCC CCCAGCCCAG CTCCTGCAGC CCGACCCCGA ATCTTATATT 
CAGAGGAACC CTCCTGAGCC CGACACTATG GAGGACCCCC AGTCTCCGGG TTCTGCATCC 
ACTCGGGGGC CAGGTGGGGC CCCTAGGGGC GGTGAGCCAG CCGTGGACGG TGACAGAGGC 
GTGGGCTCTG TCGAGCACTG TCCCCTCCAT CTGTCCAGCT GCCACGAGTG TTTGGAGCTT 
GAGAACAGCA CCATTGAATC CGTCAGGTTT GCCTCTGCAG AGAACATTCC AGATCTCCCG 
TACGACTACA GCGGCAGTTT GGAGGGCCTC GCTGACGACC TCCCCACCGA GAGGGATGGG 
AGGAGGGTCA ACGTCACGGG CAAGGCCCCC AACATCCTCC TGTATGTGGG CTCTGACTCC 
CAGGATGGCC AGGACAGGCT CCAGCAGGTC CGATCGGCGC TGGCTGACTG TGTGGACACG 
GACTGTTACA CTCTGTACCA CCTGCCTCCG GAAAGCGCTC TCAGGGACCC GTGGCCGGAC 
AACTGTCTGC TGCTGGTCAT CGCCACCGGG GAGCCCGTCC CCGAGGACCT GTCCCGGAGG 
TTCACGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGCCC TGGCTTCGTC CTTCACGCTG 
CCTGGCTTCC AGGTGACCAG CAAGGCCCCG CTGCAGGACA CGGTCCAGAG CTTGGTTTTC 
TGCAAGGTGG ATGGGAGCCT GGTGAGGCTT AGCATCCTGA GCAGTGGCTA CGTTTACGAG 
GAGGGGCCTG GGGAGCCGCT CGGCCCCAGC AAGCTCCACG GCCACCTGGA CAGTGAGGAC 
GGGGACAGGC TGGTGGTGCA AGTGCCTTTC GGGACTCATG GAGGAGAAGC TGTTCTCTGC 
CAGGTGCACT TGGAACTACC TCCCAGCTCT CCAGCAGTGC AAACACAAGA GGATTTTAAC 
CTGCTCAAAT CAAGCAACTT TAGAAGATAT GAAGTCCTTA CGGAGATCCT GACGACACTG 
GGCCTAAGCT GCGACAGAAA GCAAGCTCCT GCCTTAACGC CTCTTTACCT GCTGTCGGCC 
ACGGAGGAAA TCCGGGATCC TCTTATGCAG TGGCTGCGGA AACATGTGGA TCCTGAAGGA 
GTAATAAGAT CCAGCAAGCT CTCCCTGAAA TTCATTTCAT CCTACACGTC TGAAATAGAA 
ATCACCCCAT CCACCATACC CGTGGTGACT GACACGGAGT CCTTCTCATC AGAAAACTTT 
GACTTCGAGA TCTACCGACA AAACCTGAAG ACAAGGAAAC TTGGAAAAGT CATTCTGTTT 
GCCGAAGTGA CGCCCACCAC AATGAGTCTT CTGGACGGGT TGATGTTTGA GATGCCGCAG 
GAAATGGGTT TAATAGCCAT CGCAGTCCGC CAGACGCAGG GCAAAGGGCG GGGAAAGAAT 
GCTTGGCTGA GCCCCTTGGG ATGCGCCCTT TCCACCCTGC TGGTCTGCAT CCCGCTGAGA 
TCCCCGCTGG GACAGAGGAT TCCGTTTGTC CAGCATCTGA TGTCCCTGGC CGTCGTAGAG 
GCAGTAAGAT CCATCCCCGG GTACCAGGAT ATCAATTTGC GAGTGAAGTG GCCCAATGAT 
ATTTATTACA GTGACCTCAT GAAGCTTGGT GGAGTTTTGG TTAACTCCAC ACTTATGGGA 
GAAACATTTT ATATACTTAT TGGCTGTGGA TTTAACGTGA CGAACAGCAA CCCCACCATA 
TGCATCAATG ATCTCATCAC AGAATACAAT AAGGAACACG GGGCAGAGCT ACAGCCCTTG 
AGAGCTGACT GTCTCATCGC CCGAACGGCG ACCGTGCTGG AGAAGCTGAT CGACACGTTT 
CAGGACCAGG GGCCCAACGG CGTTCTTCCC CTCTACTATA AATACTGGGT ACACAGTGCT 
CAGCAAGTCC ACCTGGGAAG CGCTGAGGGA CCAAAGGTGT GGATAGTTGG CCTGGATGAC 
TCCGGGTTCC TTCTGGTCCA CCAGGAAAAC GGCGATGTGG TCACGGTGCA TCCTGACGGC 
AACTCCTTCG ACATGCTGAG AAACCTCATT GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960387.1
CDS	161..2608
Translation	MTQDPDARSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012104997.2
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012104997.2

ORF Insert Sequence:

ATGACCCAGG ATCCAGATGC AAGGTCCATT GATGACTTAA ACAAATGGGC CCTGTTCCTG 
GTTTCTCCTT TTATACTTGA AGCAGAGCAC ATCGCCTTTG TGACAGAGAG CATTTGGGTG 
CAAGGTGAGA ATTTACAGAG ACCAGCATCC TCTTCGGAAA CTATTGTGAA GTGGTCTGAC 
TGCTGTTTGC CGTTAGCTTG CCGACCTGGG GATCCTTATC AATTAATCGC CAAAGCAAGT 
GTGGATGACT TCAGCAAGCT AGGGGTGGCA TTCCTGGAAG ATAGACTCCA GATGGCTAAT 
GGACTGATAC CTCAGAAGAT CGTGTCTGTC CACTTGCAGG ACTCTGCCCT AAAGGAACTT 
AAGGGTCAGG CCTCAGATCC CCCAGCCCAG CTCCTGCAGC CCGACCCCGA ATCTTATATT 
CAGAGGAACC CTCCTGAGCC CGACACTATG GAGGACCCCC AGTCTCCGGG TTCTGCATCC 
ACTCGGGGGC CAGGTGGGGC CCCTAGGGGC GGTGAGCCAG CCGTGGACGG TGACAGAGGC 
GTGGGCTCTG TCGAGCACTG TCCCCTCCAT CTGTCCAGCT GCCACGAGTG TTTGGAGCTT 
GAGAACAGCA CCATTGAATC CGTCAGGTTT GCCTCTGCAG AGAACATTCC AGATCTCCCG 
TACGACTACA GCGGCAGTTT GGAGGGCCTC GCTGACGACC TCCCCACCGA GAGGGATGGG 
AGGAGGGTCA ACGTCACGGG CAAGGCCCCC AACATCCTCC TGTATGTGGG CTCTGACTCC 
CAGGATGGCC AGGACAGGCT CCAGCAGGTC CGATCGGCGC TGGCTGACTG TGTGGACACG 
GACTGTTACA CTCTGTACCA CCTGCCTCCG GAAAGCGCTC TCAGGGACCC GTGGCCGGAC 
AACTGTCTGC TGCTGGTCAT CGCCACCGGG GAGCCCGTCC CCGAGGACCT GTCCCGGAGG 
TTCACGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGCCC TGGCTTCGTC CTTCACGCTG 
CCTGGCTTCC AGGTGACCAG CAAGGCCCCG CTGCAGGACA CGGTCCAGAG CTTGGTTTTC 
TGCAAGGTGG ATGGGAGCCT GGTGAGGCTT AGCATCCTGA GCAGTGGCTA CGTTTACGAG 
GAGGGGCCTG GGGAGCCGCT CGGCCCCAGC AAGCTCCACG GCCACCTGGA CAGTGAGGAC 
GGGGACAGGC TGGTGGTGCA AGTGCCTTTC GGGACTCATG GAGGAGAAGC TGTTCTCTGC 
CAGGTGCACT TGGAACTACC TCCCAGCTCT CCAGCAGTGC AAACACAAGA GGATTTTAAC 
CTGCTCAAAT CAAGCAACTT TAGAAGATAT GAAGTCCTTA CGGAGATCCT GACGACACTG 
GGCCTAAGCT GCGACAGAAA GCAAGCTCCT GCCTTAACGC CTCTTTACCT GCTGTCGGCC 
ACGGAGGAAA TCCGGGATCC TCTTATGCAG TGGCTGCGGA AACATGTGGA TCCTGAAGGA 
GTAATAAGAT CCAGCAAGCT CTCCCTGAAA TTCATTTCAT CCTACACGTC TGAAATAGAA 
ATCACCCCAT CCACCATACC CGTGGTGACT GACACGGAGT CCTTCTCATC AGAAAACTTT 
GACTTCGAGA TCTACCGACA AAACCTGAAG ACAAGGAAAC TTGGAAAAGT CATTCTGTTT 
GCCGAAGTGA CGCCCACCAC AATGAGTCTT CTGGACGGGT TGATGTTTGA GATGCCGCAG 
GAAATGGGTT TAATAGCCAT CGCAGTCCGC CAGACGCAGG GCAAAGGGCG GGGAAAGAAT 
GCTTGGCTGA GCCCCTTGGG ATGCGCCCTT TCCACCCTGC TGGTCTGCAT CCCGCTGAGA 
TCCCCGCTGG GACAGAGGAT TCCGTTTGTC CAGCATCTGA TGTCCCTGGC CGTCGTAGAG 
GCAGTAAGAT CCATCCCCGG GTACCAGGAT ATCAATTTGC GAGTGAAGTG GCCCAATGAT 
ATTTATTACA GTGACCTCAT GAAGCTTGGT GGAGTTTTGG TTAACTCCAC ACTTATGGGA 
GAAACATTTT ATATACTTAT TGGCTGTGGA TTTAACGTGA CGAACAGCAA CCCCACCATA 
TGCATCAATG ATCTCATCAC AGAATACAAT AAGGAACACG GGGCAGAGCT ACAGCCCTTG 
AGAGCTGACT GTCTCATCGC CCGAACGGCG ACCGTGCTGG AGAAGCTGAT CGACACGTTT 
CAGGACCAGG GGCCCAACGG CGTTCTTCCC CTCTACTATA AATACTGGGT ACACAGTGCT 
CAGCAAGTCC ACCTGGGAAG CGCTGAGGGA CCAAAGGTGT GGATAGTTGG CCTGGATGAC 
TCCGGGTTCC TTCTGGTCCA CCAGGAAAAC GGCGATGTGG TCACGGTGCA TCCTGACGGC 
AACTCCTTCG ACATGCTGAG AAACCTCATT GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012105003.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960393.1 Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSm30537
Clone ID Related Accession (Same CDS sequence)	XM_012105003.2
Accession Version	XM_012105003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012105003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGG ATCCAGATGC AAGGATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCTCAGAAG 
ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAT 
CCCCCAGCCC AGCTCCTGCA GCCCGACCCC GAATCTTATA TTCAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCG GGTTCTGCAT CCACTCGGGG GCCAGGTGGG 
GCCCCTAGGG GCGGTGAGCC AGCCGTGGAC GGTGACAGAG GCGTGGGCTC TGTCGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCACGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCGGCAGT 
TTGGAGGGCC TCGCTGACGA CCTCCCCACC GAGAGGGATG GGAGGAGGGT CAACGTCACG 
GGCAAGGCCC CCAACATCCT CCTGTATGTG GGCTCTGACT CCCAGGATGG CCAGGACAGG 
CTCCAGCAGG TCCGATCGGC GCTGGCTGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC 
CACCTGCCTC CGGAAAGCGC TCTCAGGGAC CCGTGGCCGG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCACGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCTTCG TCCTTCACGC TGCCTGGCTT CCAGGTGACC 
AGCAAGGCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGT GGATGGGAGC 
CTGGTGAGGC TTAGCATCCT GAGCAGTGGC TACGTTTACG AGGAGGGGCC TGGGGAGCCG 
CTCGGCCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ACGGGGACAG GCTGGTGGTG 
CAAGTGCCTT TCGGGACTCA TGGAGGAGAA GCTGTTCTCT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGCAGT GCAAACACAA GAGGATTTTA ACCTGCTCAA ATCAAGCAAC 
TTTAGAAGAT ATGAAGTCCT TACGGAGATC CTGACGACAC TGGGCCTAAG CTGCGACAGA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGTCGG CCACGGAGGA AATCCGGGAT 
CCTCTTATGC AGTGGCTGCG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAG 
CTCTCCCTGA AATTCATTTC ATCCTACACG TCTGAAATAG AAATCACCCC ATCCACCATA 
CCCGTGGTGA CTGACACGGA GTCCTTCTCA TCAGAAAACT TTGACTTCGA GATCTACCGA 
CAAAACCTGA AGACAAGGAA ACTTGGAAAA GTCATTCTGT TTGCCGAAGT GACGCCCACC 
ACAATGAGTC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCGCTGA GATCCCCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCAGTAAG ATCCATCCCC 
GGGTACCAGG ATATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCC ACACTTATGG GAGAAACATT TTATATACTT 
ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCCACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA CGGGGCAGAG CTACAGCCCT TGAGAGCTGA CTGTCTCATC 
GCCCGAACGG CGACCGTGCT GGAGAAGCTG ATCGACACGT TTCAGGACCA GGGGCCCAAC 
GGCGTTCTTC CCCTCTACTA TAAATACTGG GTACACAGTG CTCAGCAAGT CCACCTGGGA 
AGCGCTGAGG GACCAAAGGT GTGGATAGTT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC 
CACCAGGAAA ACGGCGATGT GGTCACGGTG CATCCTGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TTGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960393.1
CDS	161..2470
Translation	MTQDPDARIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012105003.2
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012105003.2

ORF Insert Sequence:

ATGACCCAGG ATCCAGATGC AAGGATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCTCAGAAG 
ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAT 
CCCCCAGCCC AGCTCCTGCA GCCCGACCCC GAATCTTATA TTCAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCG GGTTCTGCAT CCACTCGGGG GCCAGGTGGG 
GCCCCTAGGG GCGGTGAGCC AGCCGTGGAC GGTGACAGAG GCGTGGGCTC TGTCGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCACGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCGGCAGT 
TTGGAGGGCC TCGCTGACGA CCTCCCCACC GAGAGGGATG GGAGGAGGGT CAACGTCACG 
GGCAAGGCCC CCAACATCCT CCTGTATGTG GGCTCTGACT CCCAGGATGG CCAGGACAGG 
CTCCAGCAGG TCCGATCGGC GCTGGCTGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC 
CACCTGCCTC CGGAAAGCGC TCTCAGGGAC CCGTGGCCGG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCACGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCTTCG TCCTTCACGC TGCCTGGCTT CCAGGTGACC 
AGCAAGGCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGT GGATGGGAGC 
CTGGTGAGGC TTAGCATCCT GAGCAGTGGC TACGTTTACG AGGAGGGGCC TGGGGAGCCG 
CTCGGCCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ACGGGGACAG GCTGGTGGTG 
CAAGTGCCTT TCGGGACTCA TGGAGGAGAA GCTGTTCTCT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGCAGT GCAAACACAA GAGGATTTTA ACCTGCTCAA ATCAAGCAAC 
TTTAGAAGAT ATGAAGTCCT TACGGAGATC CTGACGACAC TGGGCCTAAG CTGCGACAGA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGTCGG CCACGGAGGA AATCCGGGAT 
CCTCTTATGC AGTGGCTGCG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAG 
CTCTCCCTGA AATTCATTTC ATCCTACACG TCTGAAATAG AAATCACCCC ATCCACCATA 
CCCGTGGTGA CTGACACGGA GTCCTTCTCA TCAGAAAACT TTGACTTCGA GATCTACCGA 
CAAAACCTGA AGACAAGGAA ACTTGGAAAA GTCATTCTGT TTGCCGAAGT GACGCCCACC 
ACAATGAGTC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCGCTGA GATCCCCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCAGTAAG ATCCATCCCC 
GGGTACCAGG ATATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCC ACACTTATGG GAGAAACATT TTATATACTT 
ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCCACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA CGGGGCAGAG CTACAGCCCT TGAGAGCTGA CTGTCTCATC 
GCCCGAACGG CGACCGTGCT GGAGAAGCTG ATCGACACGT TTCAGGACCA GGGGCCCAAC 
GGCGTTCTTC CCCTCTACTA TAAATACTGG GTACACAGTG CTCAGCAAGT CCACCTGGGA 
AGCGCTGAGG GACCAAAGGT GTGGATAGTT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC 
CACCAGGAAA ACGGCGATGT GGTCACGGTG CATCCTGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TTGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012105018.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960408.1 Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OSm30538
Clone ID Related Accession (Same CDS sequence)	XM_012105018.2 , XM_012105038.2 , XM_012105031.2
Accession Version	XM_012105018.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012105018.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACACAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CACCATACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCCACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960408.1
CDS	4322..6478
Translation	MANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012105018.2
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012105018.2

ORF Insert Sequence:

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACACAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CACCATACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCCACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012105038.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960428.1 Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OSm30538
Clone ID Related Accession (Same CDS sequence)	XM_012105018.2 , XM_012105038.2 , XM_012105031.2
Accession Version	XM_012105038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012105038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACACAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CACCATACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCCACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960428.1
CDS	411..2567
Translation	MANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012105038.2
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012105038.2

ORF Insert Sequence:

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACACAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CACCATACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCCACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012105031.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960421.1 Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSm30538
Clone ID Related Accession (Same CDS sequence)	XM_012105018.2 , XM_012105038.2 , XM_012105031.2
Accession Version	XM_012105031.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012105031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACACAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CACCATACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCCACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960421.1
CDS	496..2652
Translation	MANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012105031.2
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012105031.2

ORF Insert Sequence:

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACACAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CACCATACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCCACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012105047.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960437.1 Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X8, mRNA.

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CloneID	OSm30539
Clone ID Related Accession (Same CDS sequence)	XM_012105047.2
Accession Version	XM_012105047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012105047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAAAG GGGGAGGGAG GAGGGCAGCG GGAACGGTGC TCCGGAGCGG CCAGAAGCCC 
CGGCCAATGT CAGCGGAGGC TGGAGCAGTG GGCAGGATTT GGGGTTGTGC CGGGCAGCGG 
GGCCCCCACC AAGGGGGATC TCCACCCGGC CTTTCCCACG GCCTGCTTCC CACTCCCCCG 
CGGCCACCTG CTCTGTCCTT GGGGGAACGC TTCTGCCCGC CCCCCACCCC CCTCCCAAAT 
CAGGCGCACT GGCCGCGGGG TCCCGGGGCT TGGCCCACAG GTCCCTGTTA CTGGGGGCTC 
CCTCTTAACC AGTCCATTGA TGACTTAAAC AAATGGGCCC TGTTCCTGGT TTCTCCTTTT 
ATACTTGAAG CAGAGCACAT CGCCTTTGTG ACAGAGAGCA TTTGGGTGCA AGGTGAGAAT 
TTACAGAGAC CAGCATCCTC TTCGGAAACT ATTGTGAAGT GGTCTGACTG CTGTTTGCCG 
TTAGCTTGCC GACCTGGGGA TCCTTATCAA TTAATCGCCA AAGCAAGTGT GGATGACTTC 
AGCAAGCTAG GGGTGGCATT CCTGGAAGAT AGACTCCAGA TGGCTAATGG ACTGATACCT 
CAGAAGATCG TGTCTGTCCA CTTGCAGGAC TCTGCCCTAA AGGAACTTAA GGGTCAGGCC 
TCAGATCCCC CAGCCCAGCT CCTGCAGCCC GACCCCGAAT CTTATATTCA GAGGAACCCT 
CCTGAGCCCG ACACTATGGA GGACCCCCAG TCTCCGGGTT CTGCATCCAC TCGGGGGCCA 
GGTGGGGCCC CTAGGGGCGG TGAGCCAGCC GTGGACGGTG ACAGAGGCGT GGGCTCTGTC 
GAGCACTGTC CCCTCCATCT GTCCAGCTGC CACGAGTGTT TGGAGCTTGA GAACAGCACC 
ATTGAATCCG TCAGGTTTGC CTCTGCAGAG AACATTCCAG ATCTCCCGTA CGACTACAGC 
GGCAGTTTGG AGGGCCTCGC TGACGACCTC CCCACCGAGA GGGATGGGAG GAGGGTCAAC 
GTCACGGGCA AGGCCCCCAA CATCCTCCTG TATGTGGGCT CTGACTCCCA GGATGGCCAG 
GACAGGCTCC AGCAGGTCCG ATCGGCGCTG GCTGACTGTG TGGACACGGA CTGTTACACT 
CTGTACCACC TGCCTCCGGA AAGCGCTCTC AGGGACCCGT GGCCGGACAA CTGTCTGCTG 
CTGGTCATCG CCACCGGGGA GCCCGTCCCC GAGGACCTGT CCCGGAGGTT CACGGCCTAT 
CTTTCTCAGG GAGGGAAGGT GCTGGCCCTG GCTTCGTCCT TCACGCTGCC TGGCTTCCAG 
GTGACCAGCA AGGCCCCGCT GCAGGACACG GTCCAGAGCT TGGTTTTCTG CAAGGTGGAT 
GGGAGCCTGG TGAGGCTTAG CATCCTGAGC AGTGGCTACG TTTACGAGGA GGGGCCTGGG 
GAGCCGCTCG GCCCCAGCAA GCTCCACGGC CACCTGGACA GTGAGGACGG GGACAGGCTG 
GTGGTGCAAG TGCCTTTCGG GACTCATGGA GGAGAAGCTG TTCTCTGCCA GGTGCACTTG 
GAACTACCTC CCAGCTCTCC AGCAGTGCAA ACACAAGAGG ATTTTAACCT GCTCAAATCA 
AGCAACTTTA GAAGATATGA AGTCCTTACG GAGATCCTGA CGACACTGGG CCTAAGCTGC 
GACAGAAAGC AAGCTCCTGC CTTAACGCCT CTTTACCTGC TGTCGGCCAC GGAGGAAATC 
CGGGATCCTC TTATGCAGTG GCTGCGGAAA CATGTGGATC CTGAAGGAGT AATAAGATCC 
AGCAAGCTCT CCCTGAAATT CATTTCATCC TACACGTCTG AAATAGAAAT CACCCCATCC 
ACCATACCCG TGGTGACTGA CACGGAGTCC TTCTCATCAG AAAACTTTGA CTTCGAGATC 
TACCGACAAA ACCTGAAGAC AAGGAAACTT GGAAAAGTCA TTCTGTTTGC CGAAGTGACG 
CCCACCACAA TGAGTCTTCT GGACGGGTGT CCCGGCCTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960437.1
CDS	3390..5414
Translation	MPKGGGRRAAGTVLRSGQKPRPMSAEAGAVGRIWGCAGQRGPHQGGSPPGLSHGLLPTPPRPPALSLGERFCPPPTPLPNQAHWPRGPGAWPTGPCYWGLPLNQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGCPGLC

Target ORF information:

RefSeq Version	XM_012105047.2
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012105047.2

ORF Insert Sequence:

ATGCCAAAAG GGGGAGGGAG GAGGGCAGCG GGAACGGTGC TCCGGAGCGG CCAGAAGCCC 
CGGCCAATGT CAGCGGAGGC TGGAGCAGTG GGCAGGATTT GGGGTTGTGC CGGGCAGCGG 
GGCCCCCACC AAGGGGGATC TCCACCCGGC CTTTCCCACG GCCTGCTTCC CACTCCCCCG 
CGGCCACCTG CTCTGTCCTT GGGGGAACGC TTCTGCCCGC CCCCCACCCC CCTCCCAAAT 
CAGGCGCACT GGCCGCGGGG TCCCGGGGCT TGGCCCACAG GTCCCTGTTA CTGGGGGCTC 
CCTCTTAACC AGTCCATTGA TGACTTAAAC AAATGGGCCC TGTTCCTGGT TTCTCCTTTT 
ATACTTGAAG CAGAGCACAT CGCCTTTGTG ACAGAGAGCA TTTGGGTGCA AGGTGAGAAT 
TTACAGAGAC CAGCATCCTC TTCGGAAACT ATTGTGAAGT GGTCTGACTG CTGTTTGCCG 
TTAGCTTGCC GACCTGGGGA TCCTTATCAA TTAATCGCCA AAGCAAGTGT GGATGACTTC 
AGCAAGCTAG GGGTGGCATT CCTGGAAGAT AGACTCCAGA TGGCTAATGG ACTGATACCT 
CAGAAGATCG TGTCTGTCCA CTTGCAGGAC TCTGCCCTAA AGGAACTTAA GGGTCAGGCC 
TCAGATCCCC CAGCCCAGCT CCTGCAGCCC GACCCCGAAT CTTATATTCA GAGGAACCCT 
CCTGAGCCCG ACACTATGGA GGACCCCCAG TCTCCGGGTT CTGCATCCAC TCGGGGGCCA 
GGTGGGGCCC CTAGGGGCGG TGAGCCAGCC GTGGACGGTG ACAGAGGCGT GGGCTCTGTC 
GAGCACTGTC CCCTCCATCT GTCCAGCTGC CACGAGTGTT TGGAGCTTGA GAACAGCACC 
ATTGAATCCG TCAGGTTTGC CTCTGCAGAG AACATTCCAG ATCTCCCGTA CGACTACAGC 
GGCAGTTTGG AGGGCCTCGC TGACGACCTC CCCACCGAGA GGGATGGGAG GAGGGTCAAC 
GTCACGGGCA AGGCCCCCAA CATCCTCCTG TATGTGGGCT CTGACTCCCA GGATGGCCAG 
GACAGGCTCC AGCAGGTCCG ATCGGCGCTG GCTGACTGTG TGGACACGGA CTGTTACACT 
CTGTACCACC TGCCTCCGGA AAGCGCTCTC AGGGACCCGT GGCCGGACAA CTGTCTGCTG 
CTGGTCATCG CCACCGGGGA GCCCGTCCCC GAGGACCTGT CCCGGAGGTT CACGGCCTAT 
CTTTCTCAGG GAGGGAAGGT GCTGGCCCTG GCTTCGTCCT TCACGCTGCC TGGCTTCCAG 
GTGACCAGCA AGGCCCCGCT GCAGGACACG GTCCAGAGCT TGGTTTTCTG CAAGGTGGAT 
GGGAGCCTGG TGAGGCTTAG CATCCTGAGC AGTGGCTACG TTTACGAGGA GGGGCCTGGG 
GAGCCGCTCG GCCCCAGCAA GCTCCACGGC CACCTGGACA GTGAGGACGG GGACAGGCTG 
GTGGTGCAAG TGCCTTTCGG GACTCATGGA GGAGAAGCTG TTCTCTGCCA GGTGCACTTG 
GAACTACCTC CCAGCTCTCC AGCAGTGCAA ACACAAGAGG ATTTTAACCT GCTCAAATCA 
AGCAACTTTA GAAGATATGA AGTCCTTACG GAGATCCTGA CGACACTGGG CCTAAGCTGC 
GACAGAAAGC AAGCTCCTGC CTTAACGCCT CTTTACCTGC TGTCGGCCAC GGAGGAAATC 
CGGGATCCTC TTATGCAGTG GCTGCGGAAA CATGTGGATC CTGAAGGAGT AATAAGATCC 
AGCAAGCTCT CCCTGAAATT CATTTCATCC TACACGTCTG AAATAGAAAT CACCCCATCC 
ACCATACCCG TGGTGACTGA CACGGAGTCC TTCTCATCAG AAAACTTTGA CTTCGAGATC 
TACCGACAAA ACCTGAAGAC AAGGAAACTT GGAAAAGTCA TTCTGTTTGC CGAAGTGACG 
CCCACCACAA TGAGTCTTCT GGACGGGTGT CCCGGCCTCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012130629.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986019.1 Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.

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CloneID	OOv13969
Clone ID Related Accession (Same CDS sequence)	XM_012130629.2
Accession Version	XM_012130629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAAAG GGGGAGGGAG GAGGGCAGCG GGAACGGTGC TCCGGAGCGG CCAGAAGCCC 
CGGCCAATGT CAGCGGAGGC TGGAGCAGTG GGCAGGATTT GGGGTTGTGC CGGGCAGCGG 
GGCCCCCACC AAGGGGGATC TCCACCCGGC CTTTCCCACG GCCTGCTTCC CACTCCCCCG 
CAGCCACCTG CTCTGTCCTT GGGGGAACGC TTCTGCCCGC CCCCCACCCC CCTCCCAAAT 
CAGGCGCACT GGCCGCGGGG TCCCGGGGCT TGGCCCACAG GTCCCTGTTA CTGGGGGCTC 
CCTCTTAACC AGTCCATTGA TGACTTAAAC AAATGGGCCC TGTTCCTGGT TTCTCCTTTT 
ATACTTGAAG CAGAGCACAT CGCCTTTGTG ACAGAGAGCA TTTGGGTGCA AGGTGAGAAT 
TTACAGAGAC CAGCATCCTC TTCGGAAACT ATTGTGAAGT GGTCTGACTG CTGTTTGCCG 
TTAGCTTGCC GACCTGGGGA TCCTTATCAA TTAATCGCCA AAGCAAGTGT GGATGACTTC 
AGCAAGCTAG GGGTGGCATT CCTGGAAGAT AGACTCCAGA TGGCTAATGG ACTGATACCT 
CAGAAGATCG TGTCTGTCCA CTTGCAGGAC TCTGCCCTAA AGGAACTTAA GGGTCAGGCC 
TCAGATCCCC CAGCCCAGCT CCTGCAGCCC GACCCCGAAT CTTATATTCA GAGGAACCCT 
CCTGAGCCCG ACACTATGGA GGACCCCCAG TCTCCGGGTT CTGCATCCAC TCGGGGGCCA 
GGTGGGGCCC CTAGGGGCGG TGAGCCAGCC GTGGACGGTG ACAGAGGCGT GGGCTCTGTC 
GAGCACTGTC CCCTCCATCT GTCCAGCTGC CACGAGTGTT TGGAGCTTGA GAACAGCACC 
ATTGAATCCG TCAGGTTTGC CTCTGCAGAG AACATTCCAG ATCTCCCGTA CGACTACAGC 
GGCAGTTTGG AGGGCCTCGC TGACGACCTC CCCACCGAGA GGGATGGGAG GAGGGTCAAC 
GTCACGGGCA AGGCCCCCAA CATCCTCCTG TATGTGGGCT CTGACTCCCA GGATGGCCAG 
GACAGGCTCC AGCAGGTCCG ATCGGCGCTG GCTGACTGTG TGGACACGGA CTGTTACACT 
CTGTACCACC TGCCTCCGGA AAGCGCTCTC AGGGACCCGT GGCCGGACAA CTGTCTGCTG 
CTGGTCATCG CCACCGGGGA GCCCGTCCCT GAGGACCTGT CCCGGAGGTT CACGGCCTAT 
CTTTCTCAGG GAGGGAAGGT GCTGGCCCTG GCTTCGTCCT TCACGCTGCC TGGCTTCCAG 
GTGACCAGCA AGGCCCCGCT GCAGGACACG GTCCAGAGCT TGGTTTTCTG CAAGGTGGAT 
GGGAGCCTGG TGAGGCTTAG CATCCTGAGC AGTGGCTACG TTTACGAGGA GGGGCCTGGG 
GAGCCGCTCG GCCCCAGCAA GCTCCACGGC CACCTGGACA GTGAGGACGG GGACAGGCTG 
GTGGTGCAAG TGCCTTTCGG GACTCATGGA GGAGAAGCTG TTCTCTGCCA GGTGCACTTG 
GAACTACCTC CCAGCTCTCC AGCAGTGCAA ACGCAAGAGG ATTTTAACCT GCTCAAATCA 
AGCAACTTTA GAAGATATGA AGTCCTTACG GAGATCCTGA CGACACTGGG CCTAAGCTGC 
GACAGAAAGC AAGCTCCTGC CTTAACGCCT CTTTACCTGC TGTCGGCCAC GGAGGAAATC 
CGGGATCCTC TTATGCAGTG GCTGCGGAAA CATGTGGATC CTGAAGGAGT AATAAGATCC 
AGCAAGCTCT CCCTGAAATT CATTTCATCC TACACGTCTG AAATAGAAAT CACCCCATCC 
ATCCTACCCG TGGTGACTGA CACGGAGTCC TTCTCATCAG AAAACTTTGA CTTCGAGATC 
TACCGACAAA ACCTGAAGAC AAGGAAACTT GGAAAAGTCA TTCTGTTTGC CGAAGTGACG 
CCTACCACAA TGAGTCTTCT GGACGGGTTG ATGTTTGAGA TGCCGCAGGA AATGGGTTTA 
ATAGCCATCG CAGTCCGCCA GACGCAGGGC AAAGGGCGGG GAAAGAATGC TTGGCTGAGC 
CCCTTGGGAT GCGCCCTTTC CACCCTGCTG GTCTGCATCC CGCTGAGATC CCCGCTGGGA 
CAGAGGATTC CGTTTGTCCA GCATCTGATG TCCCTGGCCG TCGTAGAGGC AGTAAGATCC 
ATCCCCGGGT ACCAGGATAT CAATTTGCGA GTGAAGTGGC CCAATGATAT TTATTACAGT 
GACCTCATGA AGCTTGGTGG AGTTTTGGTT AACTCCACAC TTATGGGAGA AACATTTTAT 
ATACTTATTG GCTGTGGATT TAACGTGACG AACAGCAACC CCACCATATG CATCAATGAT 
CTCATCACAG AATACAATAA GGAACACGGG GCAGAGCTAC AGCCCTTGAG AGCTGACTGT 
CTCATCGCCC GAACGGCGAC CGTGCTGGAG AAGCTGATCG ACACGTTTCA GGACCAGGGG 
CCCAACGGCG TTCTTCCCCT CTACTATAAA TACTGGGTAC ACAGTGCTCA GCAAGTCCAC 
CTGGGAAGCG CTGAGGGACC AAAGGTGTGG ATAGTTGGCC TGGATGACTC CGGGTTCCTT 
CTGGTCCACC AGGAAAACGG CGATGTGGTC ACGGTGCATC CTGACGGCAA CTCCTTCGAC 
ATGCTGAGAA ACCTCATTGT CCCCAAACAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986019.1
CDS	3374..6109
Translation	MPKGGGRRAAGTVLRSGQKPRPMSAEAGAVGRIWGCAGQRGPHQGGSPPGLSHGLLPTPPQPPALSLGERFCPPPTPLPNQAHWPRGPGAWPTGPCYWGLPLNQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSILPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012130629.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130629.2

ORF Insert Sequence:

ATGCCAAAAG GGGGAGGGAG GAGGGCAGCG GGAACGGTGC TCCGGAGCGG CCAGAAGCCC 
CGGCCAATGT CAGCGGAGGC TGGAGCAGTG GGCAGGATTT GGGGTTGTGC CGGGCAGCGG 
GGCCCCCACC AAGGGGGATC TCCACCCGGC CTTTCCCACG GCCTGCTTCC CACTCCCCCG 
CAGCCACCTG CTCTGTCCTT GGGGGAACGC TTCTGCCCGC CCCCCACCCC CCTCCCAAAT 
CAGGCGCACT GGCCGCGGGG TCCCGGGGCT TGGCCCACAG GTCCCTGTTA CTGGGGGCTC 
CCTCTTAACC AGTCCATTGA TGACTTAAAC AAATGGGCCC TGTTCCTGGT TTCTCCTTTT 
ATACTTGAAG CAGAGCACAT CGCCTTTGTG ACAGAGAGCA TTTGGGTGCA AGGTGAGAAT 
TTACAGAGAC CAGCATCCTC TTCGGAAACT ATTGTGAAGT GGTCTGACTG CTGTTTGCCG 
TTAGCTTGCC GACCTGGGGA TCCTTATCAA TTAATCGCCA AAGCAAGTGT GGATGACTTC 
AGCAAGCTAG GGGTGGCATT CCTGGAAGAT AGACTCCAGA TGGCTAATGG ACTGATACCT 
CAGAAGATCG TGTCTGTCCA CTTGCAGGAC TCTGCCCTAA AGGAACTTAA GGGTCAGGCC 
TCAGATCCCC CAGCCCAGCT CCTGCAGCCC GACCCCGAAT CTTATATTCA GAGGAACCCT 
CCTGAGCCCG ACACTATGGA GGACCCCCAG TCTCCGGGTT CTGCATCCAC TCGGGGGCCA 
GGTGGGGCCC CTAGGGGCGG TGAGCCAGCC GTGGACGGTG ACAGAGGCGT GGGCTCTGTC 
GAGCACTGTC CCCTCCATCT GTCCAGCTGC CACGAGTGTT TGGAGCTTGA GAACAGCACC 
ATTGAATCCG TCAGGTTTGC CTCTGCAGAG AACATTCCAG ATCTCCCGTA CGACTACAGC 
GGCAGTTTGG AGGGCCTCGC TGACGACCTC CCCACCGAGA GGGATGGGAG GAGGGTCAAC 
GTCACGGGCA AGGCCCCCAA CATCCTCCTG TATGTGGGCT CTGACTCCCA GGATGGCCAG 
GACAGGCTCC AGCAGGTCCG ATCGGCGCTG GCTGACTGTG TGGACACGGA CTGTTACACT 
CTGTACCACC TGCCTCCGGA AAGCGCTCTC AGGGACCCGT GGCCGGACAA CTGTCTGCTG 
CTGGTCATCG CCACCGGGGA GCCCGTCCCT GAGGACCTGT CCCGGAGGTT CACGGCCTAT 
CTTTCTCAGG GAGGGAAGGT GCTGGCCCTG GCTTCGTCCT TCACGCTGCC TGGCTTCCAG 
GTGACCAGCA AGGCCCCGCT GCAGGACACG GTCCAGAGCT TGGTTTTCTG CAAGGTGGAT 
GGGAGCCTGG TGAGGCTTAG CATCCTGAGC AGTGGCTACG TTTACGAGGA GGGGCCTGGG 
GAGCCGCTCG GCCCCAGCAA GCTCCACGGC CACCTGGACA GTGAGGACGG GGACAGGCTG 
GTGGTGCAAG TGCCTTTCGG GACTCATGGA GGAGAAGCTG TTCTCTGCCA GGTGCACTTG 
GAACTACCTC CCAGCTCTCC AGCAGTGCAA ACGCAAGAGG ATTTTAACCT GCTCAAATCA 
AGCAACTTTA GAAGATATGA AGTCCTTACG GAGATCCTGA CGACACTGGG CCTAAGCTGC 
GACAGAAAGC AAGCTCCTGC CTTAACGCCT CTTTACCTGC TGTCGGCCAC GGAGGAAATC 
CGGGATCCTC TTATGCAGTG GCTGCGGAAA CATGTGGATC CTGAAGGAGT AATAAGATCC 
AGCAAGCTCT CCCTGAAATT CATTTCATCC TACACGTCTG AAATAGAAAT CACCCCATCC 
ATCCTACCCG TGGTGACTGA CACGGAGTCC TTCTCATCAG AAAACTTTGA CTTCGAGATC 
TACCGACAAA ACCTGAAGAC AAGGAAACTT GGAAAAGTCA TTCTGTTTGC CGAAGTGACG 
CCTACCACAA TGAGTCTTCT GGACGGGTTG ATGTTTGAGA TGCCGCAGGA AATGGGTTTA 
ATAGCCATCG CAGTCCGCCA GACGCAGGGC AAAGGGCGGG GAAAGAATGC TTGGCTGAGC 
CCCTTGGGAT GCGCCCTTTC CACCCTGCTG GTCTGCATCC CGCTGAGATC CCCGCTGGGA 
CAGAGGATTC CGTTTGTCCA GCATCTGATG TCCCTGGCCG TCGTAGAGGC AGTAAGATCC 
ATCCCCGGGT ACCAGGATAT CAATTTGCGA GTGAAGTGGC CCAATGATAT TTATTACAGT 
GACCTCATGA AGCTTGGTGG AGTTTTGGTT AACTCCACAC TTATGGGAGA AACATTTTAT 
ATACTTATTG GCTGTGGATT TAACGTGACG AACAGCAACC CCACCATATG CATCAATGAT 
CTCATCACAG AATACAATAA GGAACACGGG GCAGAGCTAC AGCCCTTGAG AGCTGACTGT 
CTCATCGCCC GAACGGCGAC CGTGCTGGAG AAGCTGATCG ACACGTTTCA GGACCAGGGG 
CCCAACGGCG TTCTTCCCCT CTACTATAAA TACTGGGTAC ACAGTGCTCA GCAAGTCCAC 
CTGGGAAGCG CTGAGGGACC AAAGGTGTGG ATAGTTGGCC TGGATGACTC CGGGTTCCTT 
CTGGTCCACC AGGAAAACGG CGATGTGGTC ACGGTGCATC CTGACGGCAA CTCCTTCGAC 
ATGCTGAGAA ACCTCATTGT CCCCAAACAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012130631.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986021.1 Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv13971
Clone ID Related Accession (Same CDS sequence)	XM_012130631.2
Accession Version	XM_012130631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130631.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATC TTCAGTCTTC ATGGCGGCAG TCCATTGATG ACTTAAACAA ATGGGCCCTG 
TTCCTGGTTT CTCCTTTTAT ACTTGAAGCA GAGCACATCG CCTTTGTGAC AGAGAGCATT 
TGGGTGCAAG GTGAGAATTT ACAGAGACCA GCATCCTCTT CGGAAACTAT TGTGAAGTGG 
TCTGACTGCT GTTTGCCGTT AGCTTGCCGA CCTGGGGATC CTTATCAATT AATCGCCAAA 
GCAAGTGTGG ATGACTTCAG CAAGCTAGGG GTGGCATTCC TGGAAGATAG ACTCCAGATG 
GCTAATGGAC TGATACCTCA GAAGATCGTG TCTGTCCACT TGCAGGACTC TGCCCTAAAG 
GAACTTAAGG GTCAGGCCTC AGATCCCCCA GCCCAGCTCC TGCAGCCCGA CCCCGAATCT 
TATATTCAGA GGAACCCTCC TGAGCCCGAC ACTATGGAGG ACCCCCAGTC TCCGGGTTCT 
GCATCCACTC GGGGGCCAGG TGGGGCCCCT AGGGGCGGTG AGCCAGCCGT GGACGGTGAC 
AGAGGCGTGG GCTCTGTCGA GCACTGTCCC CTCCATCTGT CCAGCTGCCA CGAGTGTTTG 
GAGCTTGAGA ACAGCACCAT TGAATCCGTC AGGTTTGCCT CTGCAGAGAA CATTCCAGAT 
CTCCCGTACG ACTACAGCGG CAGTTTGGAG GGCCTCGCTG ACGACCTCCC CACCGAGAGG 
GATGGGAGGA GGGTCAACGT CACGGGCAAG GCCCCCAACA TCCTCCTGTA TGTGGGCTCT 
GACTCCCAGG ATGGCCAGGA CAGGCTCCAG CAGGTCCGAT CGGCGCTGGC TGACTGTGTG 
GACACGGACT GTTACACTCT GTACCACCTG CCTCCGGAAA GCGCTCTCAG GGACCCGTGG 
CCGGACAACT GTCTGCTGCT GGTCATCGCC ACCGGGGAGC CCGTCCCTGA GGACCTGTCC 
CGGAGGTTCA CGGCCTATCT TTCTCAGGGA GGGAAGGTGC TGGCCCTGGC TTCGTCCTTC 
ACGCTGCCTG GCTTCCAGGT GACCAGCAAG GCCCCGCTGC AGGACACGGT CCAGAGCTTG 
GTTTTCTGCA AGGTGGATGG GAGCCTGGTG AGGCTTAGCA TCCTGAGCAG TGGCTACGTT 
TACGAGGAGG GGCCTGGGGA GCCGCTCGGC CCCAGCAAGC TCCACGGCCA CCTGGACAGT 
GAGGACGGGG ACAGGCTGGT GGTGCAAGTG CCTTTCGGGA CTCATGGAGG AGAAGCTGTT 
CTCTGCCAGG TGCACTTGGA ACTACCTCCC AGCTCTCCAG CAGTGCAAAC GCAAGAGGAT 
TTTAACCTGC TCAAATCAAG CAACTTTAGA AGATATGAAG TCCTTACGGA GATCCTGACG 
ACACTGGGCC TAAGCTGCGA CAGAAAGCAA GCTCCTGCCT TAACGCCTCT TTACCTGCTG 
TCGGCCACGG AGGAAATCCG GGATCCTCTT ATGCAGTGGC TGCGGAAACA TGTGGATCCT 
GAAGGAGTAA TAAGATCCAG CAAGCTCTCC CTGAAATTCA TTTCATCCTA CACGTCTGAA 
ATAGAAATCA CCCCATCCAT CCTACCCGTG GTGACTGACA CGGAGTCCTT CTCATCAGAA 
AACTTTGACT TCGAGATCTA CCGACAAAAC CTGAAGACAA GGAAACTTGG AAAAGTCATT 
CTGTTTGCCG AAGTGACGCC TACCACAATG AGTCTTCTGG ACGGGTTGAT GTTTGAGATG 
CCGCAGGAAA TGGGTTTAAT AGCCATCGCA GTCCGCCAGA CGCAGGGCAA AGGGCGGGGA 
AAGAATGCTT GGCTGAGCCC CTTGGGATGC GCCCTTTCCA CCCTGCTGGT CTGCATCCCG 
CTGAGATCCC CGCTGGGACA GAGGATTCCG TTTGTCCAGC ATCTGATGTC CCTGGCCGTC 
GTAGAGGCAG TAAGATCCAT CCCCGGGTAC CAGGATATCA ATTTGCGAGT GAAGTGGCCC 
AATGATATTT ATTACAGTGA CCTCATGAAG CTTGGTGGAG TTTTGGTTAA CTCCACACTT 
ATGGGAGAAA CATTTTATAT ACTTATTGGC TGTGGATTTA ACGTGACGAA CAGCAACCCC 
ACCATATGCA TCAATGATCT CATCACAGAA TACAATAAGG AACACGGGGC AGAGCTACAG 
CCCTTGAGAG CTGACTGTCT CATCGCCCGA ACGGCGACCG TGCTGGAGAA GCTGATCGAC 
ACGTTTCAGG ACCAGGGGCC CAACGGCGTT CTTCCCCTCT ACTATAAATA CTGGGTACAC 
AGTGCTCAGC AAGTCCACCT GGGAAGCGCT GAGGGACCAA AGGTGTGGAT AGTTGGCCTG 
GATGACTCCG GGTTCCTTCT GGTCCACCAG GAAAACGGCG ATGTGGTCAC GGTGCATCCT 
GACGGCAACT CCTTCGACAT GCTGAGAAAC CTCATTGTCC CCAAACAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986021.1
CDS	146..2599
Translation	MQDLQSSWRQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSILPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012130631.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130631.2

ORF Insert Sequence:

ATGCAGGATC TTCAGTCTTC ATGGCGGCAG TCCATTGATG ACTTAAACAA ATGGGCCCTG 
TTCCTGGTTT CTCCTTTTAT ACTTGAAGCA GAGCACATCG CCTTTGTGAC AGAGAGCATT 
TGGGTGCAAG GTGAGAATTT ACAGAGACCA GCATCCTCTT CGGAAACTAT TGTGAAGTGG 
TCTGACTGCT GTTTGCCGTT AGCTTGCCGA CCTGGGGATC CTTATCAATT AATCGCCAAA 
GCAAGTGTGG ATGACTTCAG CAAGCTAGGG GTGGCATTCC TGGAAGATAG ACTCCAGATG 
GCTAATGGAC TGATACCTCA GAAGATCGTG TCTGTCCACT TGCAGGACTC TGCCCTAAAG 
GAACTTAAGG GTCAGGCCTC AGATCCCCCA GCCCAGCTCC TGCAGCCCGA CCCCGAATCT 
TATATTCAGA GGAACCCTCC TGAGCCCGAC ACTATGGAGG ACCCCCAGTC TCCGGGTTCT 
GCATCCACTC GGGGGCCAGG TGGGGCCCCT AGGGGCGGTG AGCCAGCCGT GGACGGTGAC 
AGAGGCGTGG GCTCTGTCGA GCACTGTCCC CTCCATCTGT CCAGCTGCCA CGAGTGTTTG 
GAGCTTGAGA ACAGCACCAT TGAATCCGTC AGGTTTGCCT CTGCAGAGAA CATTCCAGAT 
CTCCCGTACG ACTACAGCGG CAGTTTGGAG GGCCTCGCTG ACGACCTCCC CACCGAGAGG 
GATGGGAGGA GGGTCAACGT CACGGGCAAG GCCCCCAACA TCCTCCTGTA TGTGGGCTCT 
GACTCCCAGG ATGGCCAGGA CAGGCTCCAG CAGGTCCGAT CGGCGCTGGC TGACTGTGTG 
GACACGGACT GTTACACTCT GTACCACCTG CCTCCGGAAA GCGCTCTCAG GGACCCGTGG 
CCGGACAACT GTCTGCTGCT GGTCATCGCC ACCGGGGAGC CCGTCCCTGA GGACCTGTCC 
CGGAGGTTCA CGGCCTATCT TTCTCAGGGA GGGAAGGTGC TGGCCCTGGC TTCGTCCTTC 
ACGCTGCCTG GCTTCCAGGT GACCAGCAAG GCCCCGCTGC AGGACACGGT CCAGAGCTTG 
GTTTTCTGCA AGGTGGATGG GAGCCTGGTG AGGCTTAGCA TCCTGAGCAG TGGCTACGTT 
TACGAGGAGG GGCCTGGGGA GCCGCTCGGC CCCAGCAAGC TCCACGGCCA CCTGGACAGT 
GAGGACGGGG ACAGGCTGGT GGTGCAAGTG CCTTTCGGGA CTCATGGAGG AGAAGCTGTT 
CTCTGCCAGG TGCACTTGGA ACTACCTCCC AGCTCTCCAG CAGTGCAAAC GCAAGAGGAT 
TTTAACCTGC TCAAATCAAG CAACTTTAGA AGATATGAAG TCCTTACGGA GATCCTGACG 
ACACTGGGCC TAAGCTGCGA CAGAAAGCAA GCTCCTGCCT TAACGCCTCT TTACCTGCTG 
TCGGCCACGG AGGAAATCCG GGATCCTCTT ATGCAGTGGC TGCGGAAACA TGTGGATCCT 
GAAGGAGTAA TAAGATCCAG CAAGCTCTCC CTGAAATTCA TTTCATCCTA CACGTCTGAA 
ATAGAAATCA CCCCATCCAT CCTACCCGTG GTGACTGACA CGGAGTCCTT CTCATCAGAA 
AACTTTGACT TCGAGATCTA CCGACAAAAC CTGAAGACAA GGAAACTTGG AAAAGTCATT 
CTGTTTGCCG AAGTGACGCC TACCACAATG AGTCTTCTGG ACGGGTTGAT GTTTGAGATG 
CCGCAGGAAA TGGGTTTAAT AGCCATCGCA GTCCGCCAGA CGCAGGGCAA AGGGCGGGGA 
AAGAATGCTT GGCTGAGCCC CTTGGGATGC GCCCTTTCCA CCCTGCTGGT CTGCATCCCG 
CTGAGATCCC CGCTGGGACA GAGGATTCCG TTTGTCCAGC ATCTGATGTC CCTGGCCGTC 
GTAGAGGCAG TAAGATCCAT CCCCGGGTAC CAGGATATCA ATTTGCGAGT GAAGTGGCCC 
AATGATATTT ATTACAGTGA CCTCATGAAG CTTGGTGGAG TTTTGGTTAA CTCCACACTT 
ATGGGAGAAA CATTTTATAT ACTTATTGGC TGTGGATTTA ACGTGACGAA CAGCAACCCC 
ACCATATGCA TCAATGATCT CATCACAGAA TACAATAAGG AACACGGGGC AGAGCTACAG 
CCCTTGAGAG CTGACTGTCT CATCGCCCGA ACGGCGACCG TGCTGGAGAA GCTGATCGAC 
ACGTTTCAGG ACCAGGGGCC CAACGGCGTT CTTCCCCTCT ACTATAAATA CTGGGTACAC 
AGTGCTCAGC AAGTCCACCT GGGAAGCGCT GAGGGACCAA AGGTGTGGAT AGTTGGCCTG 
GATGACTCCG GGTTCCTTCT GGTCCACCAG GAAAACGGCG ATGTGGTCAC GGTGCATCCT 
GACGGCAACT CCTTCGACAT GCTGAGAAAC CTCATTGTCC CCAAACAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012130637.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986027.1 Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOv13972
Clone ID Related Accession (Same CDS sequence)	XM_012130637.2
Accession Version	XM_012130637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	biotin--protein ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGG ATCCAGATGC AAGGTCCATT GATGACTTAA ACAAATGGGC CCTGTTCCTG 
GTTTCTCCTT TTATACTTGA AGCAGAGCAC ATCGCCTTTG TGACAGAGAG CATTTGGGTG 
CAAGGTGAGA ATTTACAGAG ACCAGCATCC TCTTCGGAAA CTATTGTGAA GTGGTCTGAC 
TGCTGTTTGC CGTTAGCTTG CCGACCTGGG GATCCTTATC AATTAATCGC CAAAGCAAGT 
GTGGATGACT TCAGCAAGCT AGGGGTGGCA TTCCTGGAAG ATAGACTCCA GATGGCTAAT 
GGACTGATAC CTCAGAAGAT CGTGTCTGTC CACTTGCAGG ACTCTGCCCT AAAGGAACTT 
AAGGGTCAGG CCTCAGATCC CCCAGCCCAG CTCCTGCAGC CCGACCCCGA ATCTTATATT 
CAGAGGAACC CTCCTGAGCC CGACACTATG GAGGACCCCC AGTCTCCGGG TTCTGCATCC 
ACTCGGGGGC CAGGTGGGGC CCCTAGGGGC GGTGAGCCAG CCGTGGACGG TGACAGAGGC 
GTGGGCTCTG TCGAGCACTG TCCCCTCCAT CTGTCCAGCT GCCACGAGTG TTTGGAGCTT 
GAGAACAGCA CCATTGAATC CGTCAGGTTT GCCTCTGCAG AGAACATTCC AGATCTCCCG 
TACGACTACA GCGGCAGTTT GGAGGGCCTC GCTGACGACC TCCCCACCGA GAGGGATGGG 
AGGAGGGTCA ACGTCACGGG CAAGGCCCCC AACATCCTCC TGTATGTGGG CTCTGACTCC 
CAGGATGGCC AGGACAGGCT CCAGCAGGTC CGATCGGCGC TGGCTGACTG TGTGGACACG 
GACTGTTACA CTCTGTACCA CCTGCCTCCG GAAAGCGCTC TCAGGGACCC GTGGCCGGAC 
AACTGTCTGC TGCTGGTCAT CGCCACCGGG GAGCCCGTCC CTGAGGACCT GTCCCGGAGG 
TTCACGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGCCC TGGCTTCGTC CTTCACGCTG 
CCTGGCTTCC AGGTGACCAG CAAGGCCCCG CTGCAGGACA CGGTCCAGAG CTTGGTTTTC 
TGCAAGGTGG ATGGGAGCCT GGTGAGGCTT AGCATCCTGA GCAGTGGCTA CGTTTACGAG 
GAGGGGCCTG GGGAGCCGCT CGGCCCCAGC AAGCTCCACG GCCACCTGGA CAGTGAGGAC 
GGGGACAGGC TGGTGGTGCA AGTGCCTTTC GGGACTCATG GAGGAGAAGC TGTTCTCTGC 
CAGGTGCACT TGGAACTACC TCCCAGCTCT CCAGCAGTGC AAACGCAAGA GGATTTTAAC 
CTGCTCAAAT CAAGCAACTT TAGAAGATAT GAAGTCCTTA CGGAGATCCT GACGACACTG 
GGCCTAAGCT GCGACAGAAA GCAAGCTCCT GCCTTAACGC CTCTTTACCT GCTGTCGGCC 
ACGGAGGAAA TCCGGGATCC TCTTATGCAG TGGCTGCGGA AACATGTGGA TCCTGAAGGA 
GTAATAAGAT CCAGCAAGCT CTCCCTGAAA TTCATTTCAT CCTACACGTC TGAAATAGAA 
ATCACCCCAT CCATCCTACC CGTGGTGACT GACACGGAGT CCTTCTCATC AGAAAACTTT 
GACTTCGAGA TCTACCGACA AAACCTGAAG ACAAGGAAAC TTGGAAAAGT CATTCTGTTT 
GCCGAAGTGA CGCCTACCAC AATGAGTCTT CTGGACGGGT TGATGTTTGA GATGCCGCAG 
GAAATGGGTT TAATAGCCAT CGCAGTCCGC CAGACGCAGG GCAAAGGGCG GGGAAAGAAT 
GCTTGGCTGA GCCCCTTGGG ATGCGCCCTT TCCACCCTGC TGGTCTGCAT CCCGCTGAGA 
TCCCCGCTGG GACAGAGGAT TCCGTTTGTC CAGCATCTGA TGTCCCTGGC CGTCGTAGAG 
GCAGTAAGAT CCATCCCCGG GTACCAGGAT ATCAATTTGC GAGTGAAGTG GCCCAATGAT 
ATTTATTACA GTGACCTCAT GAAGCTTGGT GGAGTTTTGG TTAACTCCAC ACTTATGGGA 
GAAACATTTT ATATACTTAT TGGCTGTGGA TTTAACGTGA CGAACAGCAA CCCCACCATA 
TGCATCAATG ATCTCATCAC AGAATACAAT AAGGAACACG GGGCAGAGCT ACAGCCCTTG 
AGAGCTGACT GTCTCATCGC CCGAACGGCG ACCGTGCTGG AGAAGCTGAT CGACACGTTT 
CAGGACCAGG GGCCCAACGG CGTTCTTCCC CTCTACTATA AATACTGGGT ACACAGTGCT 
CAGCAAGTCC ACCTGGGAAG CGCTGAGGGA CCAAAGGTGT GGATAGTTGG CCTGGATGAC 
TCCGGGTTCC TTCTGGTCCA CCAGGAAAAC GGCGATGTGG TCACGGTGCA TCCTGACGGC 
AACTCCTTCG ACATGCTGAG AAACCTCATT GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986027.1
CDS	161..2608
Translation	MTQDPDARSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSILPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012130637.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130637.2

ORF Insert Sequence:

ATGACCCAGG ATCCAGATGC AAGGTCCATT GATGACTTAA ACAAATGGGC CCTGTTCCTG 
GTTTCTCCTT TTATACTTGA AGCAGAGCAC ATCGCCTTTG TGACAGAGAG CATTTGGGTG 
CAAGGTGAGA ATTTACAGAG ACCAGCATCC TCTTCGGAAA CTATTGTGAA GTGGTCTGAC 
TGCTGTTTGC CGTTAGCTTG CCGACCTGGG GATCCTTATC AATTAATCGC CAAAGCAAGT 
GTGGATGACT TCAGCAAGCT AGGGGTGGCA TTCCTGGAAG ATAGACTCCA GATGGCTAAT 
GGACTGATAC CTCAGAAGAT CGTGTCTGTC CACTTGCAGG ACTCTGCCCT AAAGGAACTT 
AAGGGTCAGG CCTCAGATCC CCCAGCCCAG CTCCTGCAGC CCGACCCCGA ATCTTATATT 
CAGAGGAACC CTCCTGAGCC CGACACTATG GAGGACCCCC AGTCTCCGGG TTCTGCATCC 
ACTCGGGGGC CAGGTGGGGC CCCTAGGGGC GGTGAGCCAG CCGTGGACGG TGACAGAGGC 
GTGGGCTCTG TCGAGCACTG TCCCCTCCAT CTGTCCAGCT GCCACGAGTG TTTGGAGCTT 
GAGAACAGCA CCATTGAATC CGTCAGGTTT GCCTCTGCAG AGAACATTCC AGATCTCCCG 
TACGACTACA GCGGCAGTTT GGAGGGCCTC GCTGACGACC TCCCCACCGA GAGGGATGGG 
AGGAGGGTCA ACGTCACGGG CAAGGCCCCC AACATCCTCC TGTATGTGGG CTCTGACTCC 
CAGGATGGCC AGGACAGGCT CCAGCAGGTC CGATCGGCGC TGGCTGACTG TGTGGACACG 
GACTGTTACA CTCTGTACCA CCTGCCTCCG GAAAGCGCTC TCAGGGACCC GTGGCCGGAC 
AACTGTCTGC TGCTGGTCAT CGCCACCGGG GAGCCCGTCC CTGAGGACCT GTCCCGGAGG 
TTCACGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGCCC TGGCTTCGTC CTTCACGCTG 
CCTGGCTTCC AGGTGACCAG CAAGGCCCCG CTGCAGGACA CGGTCCAGAG CTTGGTTTTC 
TGCAAGGTGG ATGGGAGCCT GGTGAGGCTT AGCATCCTGA GCAGTGGCTA CGTTTACGAG 
GAGGGGCCTG GGGAGCCGCT CGGCCCCAGC AAGCTCCACG GCCACCTGGA CAGTGAGGAC 
GGGGACAGGC TGGTGGTGCA AGTGCCTTTC GGGACTCATG GAGGAGAAGC TGTTCTCTGC 
CAGGTGCACT TGGAACTACC TCCCAGCTCT CCAGCAGTGC AAACGCAAGA GGATTTTAAC 
CTGCTCAAAT CAAGCAACTT TAGAAGATAT GAAGTCCTTA CGGAGATCCT GACGACACTG 
GGCCTAAGCT GCGACAGAAA GCAAGCTCCT GCCTTAACGC CTCTTTACCT GCTGTCGGCC 
ACGGAGGAAA TCCGGGATCC TCTTATGCAG TGGCTGCGGA AACATGTGGA TCCTGAAGGA 
GTAATAAGAT CCAGCAAGCT CTCCCTGAAA TTCATTTCAT CCTACACGTC TGAAATAGAA 
ATCACCCCAT CCATCCTACC CGTGGTGACT GACACGGAGT CCTTCTCATC AGAAAACTTT 
GACTTCGAGA TCTACCGACA AAACCTGAAG ACAAGGAAAC TTGGAAAAGT CATTCTGTTT 
GCCGAAGTGA CGCCTACCAC AATGAGTCTT CTGGACGGGT TGATGTTTGA GATGCCGCAG 
GAAATGGGTT TAATAGCCAT CGCAGTCCGC CAGACGCAGG GCAAAGGGCG GGGAAAGAAT 
GCTTGGCTGA GCCCCTTGGG ATGCGCCCTT TCCACCCTGC TGGTCTGCAT CCCGCTGAGA 
TCCCCGCTGG GACAGAGGAT TCCGTTTGTC CAGCATCTGA TGTCCCTGGC CGTCGTAGAG 
GCAGTAAGAT CCATCCCCGG GTACCAGGAT ATCAATTTGC GAGTGAAGTG GCCCAATGAT 
ATTTATTACA GTGACCTCAT GAAGCTTGGT GGAGTTTTGG TTAACTCCAC ACTTATGGGA 
GAAACATTTT ATATACTTAT TGGCTGTGGA TTTAACGTGA CGAACAGCAA CCCCACCATA 
TGCATCAATG ATCTCATCAC AGAATACAAT AAGGAACACG GGGCAGAGCT ACAGCCCTTG 
AGAGCTGACT GTCTCATCGC CCGAACGGCG ACCGTGCTGG AGAAGCTGAT CGACACGTTT 
CAGGACCAGG GGCCCAACGG CGTTCTTCCC CTCTACTATA AATACTGGGT ACACAGTGCT 
CAGCAAGTCC ACCTGGGAAG CGCTGAGGGA CCAAAGGTGT GGATAGTTGG CCTGGATGAC 
TCCGGGTTCC TTCTGGTCCA CCAGGAAAAC GGCGATGTGG TCACGGTGCA TCCTGACGGC 
AACTCCTTCG ACATGCTGAG AAACCTCATT GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012130632.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986022.1 Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.

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CloneID	OOv13973
Clone ID Related Accession (Same CDS sequence)	XM_012130632.2
Accession Version	XM_012130632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	biotin--protein ligase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGG ATCCAGATGC AAGGATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCTCAGAAG 
ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAT 
CCCCCAGCCC AGCTCCTGCA GCCCGACCCC GAATCTTATA TTCAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCG GGTTCTGCAT CCACTCGGGG GCCAGGTGGG 
GCCCCTAGGG GCGGTGAGCC AGCCGTGGAC GGTGACAGAG GCGTGGGCTC TGTCGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCACGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCGGCAGT 
TTGGAGGGCC TCGCTGACGA CCTCCCCACC GAGAGGGATG GGAGGAGGGT CAACGTCACG 
GGCAAGGCCC CCAACATCCT CCTGTATGTG GGCTCTGACT CCCAGGATGG CCAGGACAGG 
CTCCAGCAGG TCCGATCGGC GCTGGCTGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC 
CACCTGCCTC CGGAAAGCGC TCTCAGGGAC CCGTGGCCGG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCTGAGGAC CTGTCCCGGA GGTTCACGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCTTCG TCCTTCACGC TGCCTGGCTT CCAGGTGACC 
AGCAAGGCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGT GGATGGGAGC 
CTGGTGAGGC TTAGCATCCT GAGCAGTGGC TACGTTTACG AGGAGGGGCC TGGGGAGCCG 
CTCGGCCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ACGGGGACAG GCTGGTGGTG 
CAAGTGCCTT TCGGGACTCA TGGAGGAGAA GCTGTTCTCT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGCAGT GCAAACGCAA GAGGATTTTA ACCTGCTCAA ATCAAGCAAC 
TTTAGAAGAT ATGAAGTCCT TACGGAGATC CTGACGACAC TGGGCCTAAG CTGCGACAGA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGTCGG CCACGGAGGA AATCCGGGAT 
CCTCTTATGC AGTGGCTGCG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAG 
CTCTCCCTGA AATTCATTTC ATCCTACACG TCTGAAATAG AAATCACCCC ATCCATCCTA 
CCCGTGGTGA CTGACACGGA GTCCTTCTCA TCAGAAAACT TTGACTTCGA GATCTACCGA 
CAAAACCTGA AGACAAGGAA ACTTGGAAAA GTCATTCTGT TTGCCGAAGT GACGCCTACC 
ACAATGAGTC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCGCTGA GATCCCCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCAGTAAG ATCCATCCCC 
GGGTACCAGG ATATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCC ACACTTATGG GAGAAACATT TTATATACTT 
ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCCACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA CGGGGCAGAG CTACAGCCCT TGAGAGCTGA CTGTCTCATC 
GCCCGAACGG CGACCGTGCT GGAGAAGCTG ATCGACACGT TTCAGGACCA GGGGCCCAAC 
GGCGTTCTTC CCCTCTACTA TAAATACTGG GTACACAGTG CTCAGCAAGT CCACCTGGGA 
AGCGCTGAGG GACCAAAGGT GTGGATAGTT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC 
CACCAGGAAA ACGGCGATGT GGTCACGGTG CATCCTGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TTGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986022.1
CDS	161..2470
Translation	MTQDPDARIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSILPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012130632.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130632.2

ORF Insert Sequence:

ATGACCCAGG ATCCAGATGC AAGGATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCTCAGAAG 
ATCGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAT 
CCCCCAGCCC AGCTCCTGCA GCCCGACCCC GAATCTTATA TTCAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCG GGTTCTGCAT CCACTCGGGG GCCAGGTGGG 
GCCCCTAGGG GCGGTGAGCC AGCCGTGGAC GGTGACAGAG GCGTGGGCTC TGTCGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCACGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCCGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCGGCAGT 
TTGGAGGGCC TCGCTGACGA CCTCCCCACC GAGAGGGATG GGAGGAGGGT CAACGTCACG 
GGCAAGGCCC CCAACATCCT CCTGTATGTG GGCTCTGACT CCCAGGATGG CCAGGACAGG 
CTCCAGCAGG TCCGATCGGC GCTGGCTGAC TGTGTGGACA CGGACTGTTA CACTCTGTAC 
CACCTGCCTC CGGAAAGCGC TCTCAGGGAC CCGTGGCCGG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCTGAGGAC CTGTCCCGGA GGTTCACGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCTTCG TCCTTCACGC TGCCTGGCTT CCAGGTGACC 
AGCAAGGCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGT GGATGGGAGC 
CTGGTGAGGC TTAGCATCCT GAGCAGTGGC TACGTTTACG AGGAGGGGCC TGGGGAGCCG 
CTCGGCCCCA GCAAGCTCCA CGGCCACCTG GACAGTGAGG ACGGGGACAG GCTGGTGGTG 
CAAGTGCCTT TCGGGACTCA TGGAGGAGAA GCTGTTCTCT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGCAGT GCAAACGCAA GAGGATTTTA ACCTGCTCAA ATCAAGCAAC 
TTTAGAAGAT ATGAAGTCCT TACGGAGATC CTGACGACAC TGGGCCTAAG CTGCGACAGA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGTCGG CCACGGAGGA AATCCGGGAT 
CCTCTTATGC AGTGGCTGCG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCCAGCAAG 
CTCTCCCTGA AATTCATTTC ATCCTACACG TCTGAAATAG AAATCACCCC ATCCATCCTA 
CCCGTGGTGA CTGACACGGA GTCCTTCTCA TCAGAAAACT TTGACTTCGA GATCTACCGA 
CAAAACCTGA AGACAAGGAA ACTTGGAAAA GTCATTCTGT TTGCCGAAGT GACGCCTACC 
ACAATGAGTC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCGCTGA GATCCCCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCAGTAAG ATCCATCCCC 
GGGTACCAGG ATATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCC ACACTTATGG GAGAAACATT TTATATACTT 
ATTGGCTGTG GATTTAACGT GACGAACAGC AACCCCACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA CGGGGCAGAG CTACAGCCCT TGAGAGCTGA CTGTCTCATC 
GCCCGAACGG CGACCGTGCT GGAGAAGCTG ATCGACACGT TTCAGGACCA GGGGCCCAAC 
GGCGTTCTTC CCCTCTACTA TAAATACTGG GTACACAGTG CTCAGCAAGT CCACCTGGGA 
AGCGCTGAGG GACCAAAGGT GTGGATAGTT GGCCTGGATG ACTCCGGGTT CCTTCTGGTC 
CACCAGGAAA ACGGCGATGT GGTCACGGTG CATCCTGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TTGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012130636.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986026.1 Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OOv13974
Clone ID Related Accession (Same CDS sequence)	XM_012130636.2 , XM_012130635.2
Accession Version	XM_012130636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	biotin--protein ligase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC TGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACGCAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CATCCTACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCTACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986026.1
CDS	411..2567
Translation	MANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSILPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012130636.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130636.2

ORF Insert Sequence:

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC TGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACGCAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CATCCTACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCTACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012130635.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986025.1 Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.

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CloneID	OOv13974
Clone ID Related Accession (Same CDS sequence)	XM_012130636.2 , XM_012130635.2
Accession Version	XM_012130635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	biotin--protein ligase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC TGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACGCAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CATCCTACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCTACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986025.1
CDS	495..2651
Translation	MANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSILPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012130635.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130635.2

ORF Insert Sequence:

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGACTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC TGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCCCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACGCAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CATCCTACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCTACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGCGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_027961593.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027817394.1 Ovis aries holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

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CloneID	OSm59209
Clone ID Related Accession (Same CDS sequence)	XM_027961593.1
Accession Version	XM_027961593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTGAGCC GCGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTTTCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA GACCAGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCTCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGATC CCCCAGCCCA GCTCCTGCAG CCCGACCCCG AATCTTATAT TCAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCGG GTTCTGCATC CACTCGGGGG 
CCAGGTGGGG CCCCTAGGGG CGGTGAGCCA GCCGTGGACG GTGACAGAGG CGTGGGCTCT 
GTCGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCACGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCGGCAGTT TGGAGGGCCT CGCTGACGAC CTCCCCACCG AGAGGGATGG GAGGAGGGTC 
AACGTCACGG GCAAGGCCCC CAACATCCTC CTGTATGTGG GCTCTGGCTC CCAGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCGGCG CTGGCTGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC GGAAAGCGCT CTCAGGGACC CGTGGCCGGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCACGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCTTCGT CCTTCACGCT GCCTGGCTTC 
CAGGTGACCA GCAAGGCTCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGTG 
GATGGGAGCC TGGTGAGGCT TAGCATCCTG AGCAGTGGCT ACGTTTACGA GGAGGGGCCT 
GGGGAGCCGC TCGGCCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA CGGGGACAGG 
CTGGTGGTGC AAGTGCCTTT CGGGACTCAT GGAGGAGAAG CTGTTCTCTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGCAGTG CAAACGCAAG AGGATTTTAA CCTGCTCAAA 
TCAAGCAACT TTAGAAGATA TGAAGTCCTT ACGGAGATCC TGACGACACT GGGCCTAAGC 
TGCGACAGAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGTCGGC CACGGAGGAA 
ATCCGGGATC CTCTTATGCA GTGGCTGCGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAGC TCTCCCTGAA ATTCATTTCA TCCTACACGT CTGAAATAGA AATCACCCCA 
TCCACCATAC CCGTGGTGAC TGACACGGAG TCCTTCTCAT CAGAAAACTT TGACTTCGAG 
ATCTACCGAC AAAACCTGAA GACAAGGAAA CTTGGAAAAG TCATTCTGTT TGCCGAAGTG 
ACGCCCACCA CAATGAGTCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGTGCCCT TTCCACCCTG CTGGTCTGCA TCCCGCTGAG ATCCCCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCAGTAAGA 
TCCATCCCCG GGTACCAGGA TATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCCA CACTTATGGG AGAAACATTT 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCCACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAC GGGGCAGAGC TACAGCCCTT GAGAGCTGAC 
TGTCTCATCG CCCGAACGGC GACCGTGCTG GAGAAGCTGA TCGACACGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC CCTCTACTAT AAATACTGGG TACACAGTGC TCAGCAAGTC 
CACCTGGGAA GCGCTGAGGG ACCAAAGGTG TGGATAGTTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAAA CGGCGATGTG GTCACGGTGC ATCCTGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT TGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027817394.1
CDS	25..2643
Translation	MLITLCYLYLWARWGRGPAALVRATVQRLRASRCSFTFCSAAAPPRGSRVCLSRGGRVFCVGESQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSGSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_027961593.1
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027961593.1

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTGAGCC GCGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTTTCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA GACCAGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCTCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGATC CCCCAGCCCA GCTCCTGCAG CCCGACCCCG AATCTTATAT TCAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCGG GTTCTGCATC CACTCGGGGG 
CCAGGTGGGG CCCCTAGGGG CGGTGAGCCA GCCGTGGACG GTGACAGAGG CGTGGGCTCT 
GTCGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCACGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCGGCAGTT TGGAGGGCCT CGCTGACGAC CTCCCCACCG AGAGGGATGG GAGGAGGGTC 
AACGTCACGG GCAAGGCCCC CAACATCCTC CTGTATGTGG GCTCTGGCTC CCAGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCGGCG CTGGCTGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC GGAAAGCGCT CTCAGGGACC CGTGGCCGGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCACGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCTTCGT CCTTCACGCT GCCTGGCTTC 
CAGGTGACCA GCAAGGCTCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGTG 
GATGGGAGCC TGGTGAGGCT TAGCATCCTG AGCAGTGGCT ACGTTTACGA GGAGGGGCCT 
GGGGAGCCGC TCGGCCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA CGGGGACAGG 
CTGGTGGTGC AAGTGCCTTT CGGGACTCAT GGAGGAGAAG CTGTTCTCTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGCAGTG CAAACGCAAG AGGATTTTAA CCTGCTCAAA 
TCAAGCAACT TTAGAAGATA TGAAGTCCTT ACGGAGATCC TGACGACACT GGGCCTAAGC 
TGCGACAGAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGTCGGC CACGGAGGAA 
ATCCGGGATC CTCTTATGCA GTGGCTGCGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAGC TCTCCCTGAA ATTCATTTCA TCCTACACGT CTGAAATAGA AATCACCCCA 
TCCACCATAC CCGTGGTGAC TGACACGGAG TCCTTCTCAT CAGAAAACTT TGACTTCGAG 
ATCTACCGAC AAAACCTGAA GACAAGGAAA CTTGGAAAAG TCATTCTGTT TGCCGAAGTG 
ACGCCCACCA CAATGAGTCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGTGCCCT TTCCACCCTG CTGGTCTGCA TCCCGCTGAG ATCCCCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCAGTAAGA 
TCCATCCCCG GGTACCAGGA TATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCCA CACTTATGGG AGAAACATTT 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCCACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAC GGGGCAGAGC TACAGCCCTT GAGAGCTGAC 
TGTCTCATCG CCCGAACGGC GACCGTGCTG GAGAAGCTGA TCGACACGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC CCTCTACTAT AAATACTGGG TACACAGTGC TCAGCAAGTC 
CACCTGGGAA GCGCTGAGGG ACCAAAGGTG TGGATAGTTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAAA CGGCGATGTG GTCACGGTGC ATCCTGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT TGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012104997.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011960387.2 Ovis aries holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSm59210
Clone ID Related Accession (Same CDS sequence)	XM_012104997.3
Accession Version	XM_012104997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012104997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGG ATCCAGATGC AAGGTCCATT GATGACTTAA ACAAATGGGC CCTGTTCCTG 
GTTTCTCCTT TTATACTTGA AGCAGAGCAC ATCGCCTTTG TGACAGAGAG CATTTGGGTG 
CAAGGTGAGA ATTTACAGAG ACCAGCATCC TCTTCGGAAA CTATTGTGAA GTGGTCTGAC 
TGCTGTTTGC CGTTAGCTTG CCGACCTGGG GATCCTTATC AATTAATCGC CAAAGCAAGT 
GTGGATGACT TCAGCAAGCT AGGGGTGGCA TTCCTGGAAG ATAGACTCCA GATGGCTAAT 
GGACTGATAC CTCAGAAGAT CGTGTCTGTC CACTTGCAGG ACTCTGCCCT AAAGGAACTT 
AAGGGTCAGG CCTCAGATCC CCCAGCCCAG CTCCTGCAGC CCGACCCCGA ATCTTATATT 
CAGAGGAACC CTCCTGAGCC CGACACTATG GAGGACCCCC AGTCTCCGGG TTCTGCATCC 
ACTCGGGGGC CAGGTGGGGC CCCTAGGGGC GGTGAGCCAG CCGTGGACGG TGACAGAGGC 
GTGGGCTCTG TCGAGCACTG TCCCCTCCAT CTGTCCAGCT GCCACGAGTG TTTGGAGCTT 
GAGAACAGCA CCATTGAATC CGTCAGGTTT GCCTCTGCAG AGAACATTCC AGATCTCCCG 
TACGACTACA GCGGCAGTTT GGAGGGCCTC GCTGACGACC TCCCCACCGA GAGGGATGGG 
AGGAGGGTCA ACGTCACGGG CAAGGCCCCC AACATCCTCC TGTATGTGGG CTCTGGCTCC 
CAGGATGGCC AGGACAGGCT CCAGCAGGTC CGATCGGCGC TGGCTGACTG TGTGGACACG 
GACTGTTACA CTCTGTACCA CCTGCCTCCG GAAAGCGCTC TCAGGGACCC GTGGCCGGAC 
AACTGTCTGC TGCTGGTCAT CGCCACCGGG GAGCCCGTCC CCGAGGACCT GTCCCGGAGG 
TTCACGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGCCC TGGCTTCGTC CTTCACGCTG 
CCTGGCTTCC AGGTGACCAG CAAGGCTCCG CTGCAGGACA CGGTCCAGAG CTTGGTTTTC 
TGCAAGGTGG ATGGGAGCCT GGTGAGGCTT AGCATCCTGA GCAGTGGCTA CGTTTACGAG 
GAGGGGCCTG GGGAGCCGCT CGGCCCCAGC AAGCTCCACG GCCACCTGGA CAGTGAGGAC 
GGGGACAGGC TGGTGGTGCA AGTGCCTTTC GGGACTCATG GAGGAGAAGC TGTTCTCTGC 
CAGGTGCACT TGGAACTACC TCCCAGCTCT CCAGCAGTGC AAACGCAAGA GGATTTTAAC 
CTGCTCAAAT CAAGCAACTT TAGAAGATAT GAAGTCCTTA CGGAGATCCT GACGACACTG 
GGCCTAAGCT GCGACAGAAA GCAAGCTCCT GCCTTAACGC CTCTTTACCT GCTGTCGGCC 
ACGGAGGAAA TCCGGGATCC TCTTATGCAG TGGCTGCGGA AACATGTGGA TCCTGAAGGA 
GTAATAAGAT CCAGCAAGCT CTCCCTGAAA TTCATTTCAT CCTACACGTC TGAAATAGAA 
ATCACCCCAT CCACCATACC CGTGGTGACT GACACGGAGT CCTTCTCATC AGAAAACTTT 
GACTTCGAGA TCTACCGACA AAACCTGAAG ACAAGGAAAC TTGGAAAAGT CATTCTGTTT 
GCCGAAGTGA CGCCCACCAC AATGAGTCTT CTGGACGGGT TGATGTTTGA GATGCCGCAG 
GAAATGGGTT TAATAGCCAT CGCAGTCCGC CAGACGCAGG GCAAAGGGCG GGGAAAGAAT 
GCTTGGCTGA GCCCCTTGGG ATGTGCCCTT TCCACCCTGC TGGTCTGCAT CCCGCTGAGA 
TCCCCGCTGG GACAGAGGAT TCCGTTTGTC CAGCATCTGA TGTCCCTGGC CGTCGTAGAG 
GCAGTAAGAT CCATCCCCGG GTACCAGGAT ATCAATTTGC GAGTGAAGTG GCCCAATGAT 
ATTTATTACA GTGACCTCAT GAAGCTTGGT GGAGTTTTGG TTAACTCCAC ACTTATGGGA 
GAAACATTTT ATATACTTAT TGGCTGTGGA TTTAACGTGA CGAACAGCAA CCCCACCATA 
TGCATCAATG ATCTCATCAC AGAATACAAT AAGGAACACG GGGCAGAGCT ACAGCCCTTG 
AGAGCTGACT GTCTCATCGC CCGAACGGCG ACCGTGCTGG AGAAGCTGAT CGACACGTTT 
CAGGACCAGG GGCCCAACGG CGTTCTTCCC CTCTACTATA AATACTGGGT ACACAGTGCT 
CAGCAAGTCC ACCTGGGAAG CGCTGAGGGA CCAAAGGTGT GGATAGTTGG CCTGGATGAC 
TCCGGGTTCC TTCTGGTCCA CCAGGAAAAC GGCGATGTGG TCACGGTGCA TCCTGACGGC 
AACTCCTTCG ACATGCTGAG AAACCTCATT GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011960387.2
CDS	122..2569
Translation	MTQDPDARSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSGSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012104997.3
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012104997.3

ORF Insert Sequence:

ATGACCCAGG ATCCAGATGC AAGGTCCATT GATGACTTAA ACAAATGGGC CCTGTTCCTG 
GTTTCTCCTT TTATACTTGA AGCAGAGCAC ATCGCCTTTG TGACAGAGAG CATTTGGGTG 
CAAGGTGAGA ATTTACAGAG ACCAGCATCC TCTTCGGAAA CTATTGTGAA GTGGTCTGAC 
TGCTGTTTGC CGTTAGCTTG CCGACCTGGG GATCCTTATC AATTAATCGC CAAAGCAAGT 
GTGGATGACT TCAGCAAGCT AGGGGTGGCA TTCCTGGAAG ATAGACTCCA GATGGCTAAT 
GGACTGATAC CTCAGAAGAT CGTGTCTGTC CACTTGCAGG ACTCTGCCCT AAAGGAACTT 
AAGGGTCAGG CCTCAGATCC CCCAGCCCAG CTCCTGCAGC CCGACCCCGA ATCTTATATT 
CAGAGGAACC CTCCTGAGCC CGACACTATG GAGGACCCCC AGTCTCCGGG TTCTGCATCC 
ACTCGGGGGC CAGGTGGGGC CCCTAGGGGC GGTGAGCCAG CCGTGGACGG TGACAGAGGC 
GTGGGCTCTG TCGAGCACTG TCCCCTCCAT CTGTCCAGCT GCCACGAGTG TTTGGAGCTT 
GAGAACAGCA CCATTGAATC CGTCAGGTTT GCCTCTGCAG AGAACATTCC AGATCTCCCG 
TACGACTACA GCGGCAGTTT GGAGGGCCTC GCTGACGACC TCCCCACCGA GAGGGATGGG 
AGGAGGGTCA ACGTCACGGG CAAGGCCCCC AACATCCTCC TGTATGTGGG CTCTGGCTCC 
CAGGATGGCC AGGACAGGCT CCAGCAGGTC CGATCGGCGC TGGCTGACTG TGTGGACACG 
GACTGTTACA CTCTGTACCA CCTGCCTCCG GAAAGCGCTC TCAGGGACCC GTGGCCGGAC 
AACTGTCTGC TGCTGGTCAT CGCCACCGGG GAGCCCGTCC CCGAGGACCT GTCCCGGAGG 
TTCACGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGCCC TGGCTTCGTC CTTCACGCTG 
CCTGGCTTCC AGGTGACCAG CAAGGCTCCG CTGCAGGACA CGGTCCAGAG CTTGGTTTTC 
TGCAAGGTGG ATGGGAGCCT GGTGAGGCTT AGCATCCTGA GCAGTGGCTA CGTTTACGAG 
GAGGGGCCTG GGGAGCCGCT CGGCCCCAGC AAGCTCCACG GCCACCTGGA CAGTGAGGAC 
GGGGACAGGC TGGTGGTGCA AGTGCCTTTC GGGACTCATG GAGGAGAAGC TGTTCTCTGC 
CAGGTGCACT TGGAACTACC TCCCAGCTCT CCAGCAGTGC AAACGCAAGA GGATTTTAAC 
CTGCTCAAAT CAAGCAACTT TAGAAGATAT GAAGTCCTTA CGGAGATCCT GACGACACTG 
GGCCTAAGCT GCGACAGAAA GCAAGCTCCT GCCTTAACGC CTCTTTACCT GCTGTCGGCC 
ACGGAGGAAA TCCGGGATCC TCTTATGCAG TGGCTGCGGA AACATGTGGA TCCTGAAGGA 
GTAATAAGAT CCAGCAAGCT CTCCCTGAAA TTCATTTCAT CCTACACGTC TGAAATAGAA 
ATCACCCCAT CCACCATACC CGTGGTGACT GACACGGAGT CCTTCTCATC AGAAAACTTT 
GACTTCGAGA TCTACCGACA AAACCTGAAG ACAAGGAAAC TTGGAAAAGT CATTCTGTTT 
GCCGAAGTGA CGCCCACCAC AATGAGTCTT CTGGACGGGT TGATGTTTGA GATGCCGCAG 
GAAATGGGTT TAATAGCCAT CGCAGTCCGC CAGACGCAGG GCAAAGGGCG GGGAAAGAAT 
GCTTGGCTGA GCCCCTTGGG ATGTGCCCTT TCCACCCTGC TGGTCTGCAT CCCGCTGAGA 
TCCCCGCTGG GACAGAGGAT TCCGTTTGTC CAGCATCTGA TGTCCCTGGC CGTCGTAGAG 
GCAGTAAGAT CCATCCCCGG GTACCAGGAT ATCAATTTGC GAGTGAAGTG GCCCAATGAT 
ATTTATTACA GTGACCTCAT GAAGCTTGGT GGAGTTTTGG TTAACTCCAC ACTTATGGGA 
GAAACATTTT ATATACTTAT TGGCTGTGGA TTTAACGTGA CGAACAGCAA CCCCACCATA 
TGCATCAATG ATCTCATCAC AGAATACAAT AAGGAACACG GGGCAGAGCT ACAGCCCTTG 
AGAGCTGACT GTCTCATCGC CCGAACGGCG ACCGTGCTGG AGAAGCTGAT CGACACGTTT 
CAGGACCAGG GGCCCAACGG CGTTCTTCCC CTCTACTATA AATACTGGGT ACACAGTGCT 
CAGCAAGTCC ACCTGGGAAG CGCTGAGGGA CCAAAGGTGT GGATAGTTGG CCTGGATGAC 
TCCGGGTTCC TTCTGGTCCA CCAGGAAAAC GGCGATGTGG TCACGGTGCA TCCTGACGGC 
AACTCCTTCG ACATGCTGAG AAACCTCATT GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_027961598.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027817399.1 Ovis aries holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

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CloneID	OSm59211
Clone ID Related Accession (Same CDS sequence)	XM_027961598.1
Accession Version	XM_027961598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	biotin--protein ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGGCTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCTCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACGCAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CACCATACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCCACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGTGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027817399.1
CDS	955..3111
Translation	MANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSGSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSTIPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_027961598.1
Organism	Ovis aries(sheep)
Definition	Ovis aries holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027961598.1

ORF Insert Sequence:

ATGGCTAATG GACTGATACC TCAGAAGATC GTGTCTGTCC ACTTGCAGGA CTCTGCCCTA 
AAGGAACTTA AGGGTCAGGC CTCAGATCCC CCAGCCCAGC TCCTGCAGCC CGACCCCGAA 
TCTTATATTC AGAGGAACCC TCCTGAGCCC GACACTATGG AGGACCCCCA GTCTCCGGGT 
TCTGCATCCA CTCGGGGGCC AGGTGGGGCC CCTAGGGGCG GTGAGCCAGC CGTGGACGGT 
GACAGAGGCG TGGGCTCTGT CGAGCACTGT CCCCTCCATC TGTCCAGCTG CCACGAGTGT 
TTGGAGCTTG AGAACAGCAC CATTGAATCC GTCAGGTTTG CCTCTGCAGA GAACATTCCA 
GATCTCCCGT ACGACTACAG CGGCAGTTTG GAGGGCCTCG CTGACGACCT CCCCACCGAG 
AGGGATGGGA GGAGGGTCAA CGTCACGGGC AAGGCCCCCA ACATCCTCCT GTATGTGGGC 
TCTGGCTCCC AGGATGGCCA GGACAGGCTC CAGCAGGTCC GATCGGCGCT GGCTGACTGT 
GTGGACACGG ACTGTTACAC TCTGTACCAC CTGCCTCCGG AAAGCGCTCT CAGGGACCCG 
TGGCCGGACA ACTGTCTGCT GCTGGTCATC GCCACCGGGG AGCCCGTCCC CGAGGACCTG 
TCCCGGAGGT TCACGGCCTA TCTTTCTCAG GGAGGGAAGG TGCTGGCCCT GGCTTCGTCC 
TTCACGCTGC CTGGCTTCCA GGTGACCAGC AAGGCTCCGC TGCAGGACAC GGTCCAGAGC 
TTGGTTTTCT GCAAGGTGGA TGGGAGCCTG GTGAGGCTTA GCATCCTGAG CAGTGGCTAC 
GTTTACGAGG AGGGGCCTGG GGAGCCGCTC GGCCCCAGCA AGCTCCACGG CCACCTGGAC 
AGTGAGGACG GGGACAGGCT GGTGGTGCAA GTGCCTTTCG GGACTCATGG AGGAGAAGCT 
GTTCTCTGCC AGGTGCACTT GGAACTACCT CCCAGCTCTC CAGCAGTGCA AACGCAAGAG 
GATTTTAACC TGCTCAAATC AAGCAACTTT AGAAGATATG AAGTCCTTAC GGAGATCCTG 
ACGACACTGG GCCTAAGCTG CGACAGAAAG CAAGCTCCTG CCTTAACGCC TCTTTACCTG 
CTGTCGGCCA CGGAGGAAAT CCGGGATCCT CTTATGCAGT GGCTGCGGAA ACATGTGGAT 
CCTGAAGGAG TAATAAGATC CAGCAAGCTC TCCCTGAAAT TCATTTCATC CTACACGTCT 
GAAATAGAAA TCACCCCATC CACCATACCC GTGGTGACTG ACACGGAGTC CTTCTCATCA 
GAAAACTTTG ACTTCGAGAT CTACCGACAA AACCTGAAGA CAAGGAAACT TGGAAAAGTC 
ATTCTGTTTG CCGAAGTGAC GCCCACCACA ATGAGTCTTC TGGACGGGTT GATGTTTGAG 
ATGCCGCAGG AAATGGGTTT AATAGCCATC GCAGTCCGCC AGACGCAGGG CAAAGGGCGG 
GGAAAGAATG CTTGGCTGAG CCCCTTGGGA TGTGCCCTTT CCACCCTGCT GGTCTGCATC 
CCGCTGAGAT CCCCGCTGGG ACAGAGGATT CCGTTTGTCC AGCATCTGAT GTCCCTGGCC 
GTCGTAGAGG CAGTAAGATC CATCCCCGGG TACCAGGATA TCAATTTGCG AGTGAAGTGG 
CCCAATGATA TTTATTACAG TGACCTCATG AAGCTTGGTG GAGTTTTGGT TAACTCCACA 
CTTATGGGAG AAACATTTTA TATACTTATT GGCTGTGGAT TTAACGTGAC GAACAGCAAC 
CCCACCATAT GCATCAATGA TCTCATCACA GAATACAATA AGGAACACGG GGCAGAGCTA 
CAGCCCTTGA GAGCTGACTG TCTCATCGCC CGAACGGCGA CCGTGCTGGA GAAGCTGATC 
GACACGTTTC AGGACCAGGG GCCCAACGGC GTTCTTCCCC TCTACTATAA ATACTGGGTA 
CACAGTGCTC AGCAAGTCCA CCTGGGAAGC GCTGAGGGAC CAAAGGTGTG GATAGTTGGC 
CTGGATGACT CCGGGTTCCT TCTGGTCCAC CAGGAAAACG GCGATGTGGT CACGGTGCAT 
CCTGACGGCA ACTCCTTCGA CATGCTGAGA AACCTCATTG TCCCCAAACA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_012130630.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011986020.1 Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X9, mRNA.

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CloneID	OOv13970
Clone ID Related Accession (Same CDS sequence)	XM_012130630.2
Accession Version	XM_012130630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	biotin--protein ligase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012130630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTGAGCC GCGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTTTCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA GACCAGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCTCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGATC CCCCAGCCCA GCTCCTGCAG CCCGACCCCG AATCTTATAT TCAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCGG GTTCTGCATC CACTCGGGGG 
CCAGGTGGGG CCCCTAGGGG CGGTGAGCCA GCCGTGGACG GTGACAGAGG CGTGGGCTCT 
GTCGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCACGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCGGCAGTT TGGAGGGCCT CGCTGACGAC CTCCCCACCG AGAGGGATGG GAGGAGGGTC 
AACGTCACGG GCAAGGCCCC CAACATCCTC CTGTATGTGG GCTCTGACTC CCAGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCGGCG CTGGCTGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC GGAAAGCGCT CTCAGGGACC CGTGGCCGGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCTGAGGACC TGTCCCGGAG GTTCACGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCTTCGT CCTTCACGCT GCCTGGCTTC 
CAGGTGACCA GCAAGGCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGTG 
GATGGGAGCC TGGTGAGGCT TAGCATCCTG AGCAGTGGCT ACGTTTACGA GGAGGGGCCT 
GGGGAGCCGC TCGGCCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA CGGGGACAGG 
CTGGTGGTGC AAGTGCCTTT CGGGACTCAT GGAGGAGAAG CTGTTCTCTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGCAGTG CAAACGCAAG AGGATTTTAA CCTGCTCAAA 
TCAAGCAACT TTAGAAGATA TGAAGTCCTT ACGGAGATCC TGACGACACT GGGCCTAAGC 
TGCGACAGAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGTCGGC CACGGAGGAA 
ATCCGGGATC CTCTTATGCA GTGGCTGCGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAGC TCTCCCTGAA ATTCATTTCA TCCTACACGT CTGAAATAGA AATCACCCCA 
TCCATCCTAC CCGTGGTGAC TGACACGGAG TCCTTCTCAT CAGAAAACTT TGACTTCGAG 
ATCTACCGAC AAAACCTGAA GACAAGGAAA CTTGGAAAAG TCATTCTGTT TGCCGAAGTG 
ACGCCTACCA CAATGAGTCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCGCTGAG ATCCCCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCAGTAAGA 
TCCATCCCCG GGTACCAGGA TATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCCA CACTTATGGG AGAAACATTT 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCCACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAC GGGGCAGAGC TACAGCCCTT GAGAGCTGAC 
TGTCTCATCG CCCGAACGGC GACCGTGCTG GAGAAGCTGA TCGACACGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC CCTCTACTAT AAATACTGGG TACACAGTGC TCAGCAAGTC 
CACCTGGGAA GCGCTGAGGG ACCAAAGGTG TGGATAGTTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAAA CGGCGATGTG GTCACGGTGC ATCCTGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT TGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011986020.1
CDS	55..2673
Translation	MLITLCYLYLWARWGRGPAALVRATVQRLRASRCSFTFCSAAAPPRGSRVCLSRGGRVFCVGESQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESYIQRNPPEPDTMEDPQSPGSASTRGPGGAPRGGEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSGSLEGLADDLPTERDGRRVNVTGKAPNILLYVGSDSQDGQDRLQQVRSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLVRLSILSSGYVYEEGPGEPLGPSKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPAVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDRKQAPALTPLYLLSATEEIRDPLMQWLRKHVDPEGVIRSSKLSLKFISSYTSEIEITPSILPVVTDTESFSSENFDFEIYRQNLKTRKLGKVILFAEVTPTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTATVLEKLIDTFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_012130630.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012130630.2

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTGAGCC GCGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTTTCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA GACCAGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCTCAGAAGA TCGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGATC CCCCAGCCCA GCTCCTGCAG CCCGACCCCG AATCTTATAT TCAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCGG GTTCTGCATC CACTCGGGGG 
CCAGGTGGGG CCCCTAGGGG CGGTGAGCCA GCCGTGGACG GTGACAGAGG CGTGGGCTCT 
GTCGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCACGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CCGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCGGCAGTT TGGAGGGCCT CGCTGACGAC CTCCCCACCG AGAGGGATGG GAGGAGGGTC 
AACGTCACGG GCAAGGCCCC CAACATCCTC CTGTATGTGG GCTCTGACTC CCAGGATGGC 
CAGGACAGGC TCCAGCAGGT CCGATCGGCG CTGGCTGACT GTGTGGACAC GGACTGTTAC 
ACTCTGTACC ACCTGCCTCC GGAAAGCGCT CTCAGGGACC CGTGGCCGGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCTGAGGACC TGTCCCGGAG GTTCACGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCTTCGT CCTTCACGCT GCCTGGCTTC 
CAGGTGACCA GCAAGGCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGTG 
GATGGGAGCC TGGTGAGGCT TAGCATCCTG AGCAGTGGCT ACGTTTACGA GGAGGGGCCT 
GGGGAGCCGC TCGGCCCCAG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA CGGGGACAGG 
CTGGTGGTGC AAGTGCCTTT CGGGACTCAT GGAGGAGAAG CTGTTCTCTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGCAGTG CAAACGCAAG AGGATTTTAA CCTGCTCAAA 
TCAAGCAACT TTAGAAGATA TGAAGTCCTT ACGGAGATCC TGACGACACT GGGCCTAAGC 
TGCGACAGAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGTCGGC CACGGAGGAA 
ATCCGGGATC CTCTTATGCA GTGGCTGCGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCCAGCAAGC TCTCCCTGAA ATTCATTTCA TCCTACACGT CTGAAATAGA AATCACCCCA 
TCCATCCTAC CCGTGGTGAC TGACACGGAG TCCTTCTCAT CAGAAAACTT TGACTTCGAG 
ATCTACCGAC AAAACCTGAA GACAAGGAAA CTTGGAAAAG TCATTCTGTT TGCCGAAGTG 
ACGCCTACCA CAATGAGTCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCGCTGAG ATCCCCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCAGTAAGA 
TCCATCCCCG GGTACCAGGA TATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCCA CACTTATGGG AGAAACATTT 
TATATACTTA TTGGCTGTGG ATTTAACGTG ACGAACAGCA ACCCCACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAC GGGGCAGAGC TACAGCCCTT GAGAGCTGAC 
TGTCTCATCG CCCGAACGGC GACCGTGCTG GAGAAGCTGA TCGACACGTT TCAGGACCAG 
GGGCCCAACG GCGTTCTTCC CCTCTACTAT AAATACTGGG TACACAGTGC TCAGCAAGTC 
CACCTGGGAA GCGCTGAGGG ACCAAAGGTG TGGATAGTTG GCCTGGATGA CTCCGGGTTC 
CTTCTGGTCC ACCAGGAAAA CGGCGATGTG GTCACGGTGC ATCCTGACGG CAACTCCTTC 
GACATGCTGA GAAACCTCAT TGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HLCS
Entrez Gene ID	101109588
Full Name	holocarboxylase synthetase
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HLCS cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

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