APPL1 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	APPL1
Entrez Gene ID	101109218
Full Name	adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012099844.2	XP_011955234.1	DCC-interacting protein 13-alpha isoform X1
XM_012126241.2	XP_011981631.1	DCC-interacting protein 13-alpha isoform X2
XM_027958257.1	XP_027814058.1	DCC-interacting protein 13-alpha

Pathways from BioSystems
Gene Ontology

The following APPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm30165	XM_012099844.2 Latest version!	Ovis aries adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OOv11185	XM_012126241.2 Latest version!	Ovis aries musimon adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OSm69013	XM_027958257.1 Latest version!	Ovis aries adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APPL1 ( XM_012099844.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011955234.1 Ovis aries adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm30165
Clone ID Related Accession (Same CDS sequence)	XM_012099844.2
Accession Version	XM_012099844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	DCC-interacting protein 13-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639028.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012099844.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGT TGTATCAGGC TATGCATCGG ATTTATGATG CACAGAATGA ATTAAGTGCA 
GCAACACACC TGACTTCAAA ACTTTTAAAA GAATATGAGA AACAGCGTTT TCCACTGGGG 
GGTGATGATG AAGTTATGAG TTCTACTTTG CAGCAGTTTT CAAAAGTTAT AGATGAGCTT 
AGCTCTTGTC ATGCGGTACT TTCAACACAG CTTGCTGATG CCATGATGTT CCCCATTACC 
CAGTTTAAAG AAAGAGATCT GAAAGAAATA TTGACATTAA AGGAAGTGTT TCAGATTGCT 
AGTAATGATC ATGATGCTGC AATTAACAGA TACAGCAGAT TGTCCAAAAA AAGAGAAAAT 
GACAAGGTGA AGTATGAAGT AACAGAAGAT GTCTACACTT CGAGAAAAAA ACAACACCAG 
ACCATGATGC ATTATTTTTG TGCGTTAAAT ACTCTTCAGT ACAAAAAGAA AATAGCATTG 
TTAGAACCTC TTCTGGGATA TATGCAAGCT CAGATAAGTT TCTTTAAGAT GGGTTCTGAA 
AATCTCAATG ACCAACTGGA AGAATTCTTA ACTAATATTG GAACAAGTGT ACAGAATGTT 
CGCAGGGAAA TGGACAGCGA TGTAGAGACC ATGCAGCAGA CAATAGAGGA CTTGGAAGTG 
GCCAGTGACC CCTTATATGT GCCTGACCCG GATCCCACAA AGTTTCCTGT TCATCGAAAC 
CTGACCCGGA AGGCGGGGTA CCTTAATGCT AGGAATAAAA CAGGCTTGGT GTCATCTACC 
TGGGACAGAC AGTTTTACTT CACGCAGGGT GGAAACCTGA TGAGTCAGGC CCGTGGCGAC 
GTAGCAGGAG GCCTGGCCAT GGACATAGAC AACTGCTCAG TCATGGCTGT GGACTGTGAA 
GACAGGCGGT ACTGTTTTCA GATCACTTCT TTCGACGGCA AAAAGTCTTC AATTTTGCAA 
GCAGAGAGTA AAAAAGACCA TGAAGAGTGG ATCTGTACAA TAAATAACAT ATCTAAACAA 
ATATATTTAA GTGAAAACCC AGAGGAGATT GCTGCACGAG TAAATCAGTC AGCTTTGGAA 
GCTGTCACTC CTTCCCCATC TTTCCAGCAG AGGCACGAGA GCCTGAGGCC GGCAGGACAA 
TCTCGGCCAC CCACAGCTCG AACCAGCAGT TCAGGATCCT TAGGATCTGA GTCCACAAAT 
TTGGCTGCCC TCTCTCTAGA TTCTCTTGTT GCCCCAGATA CCCCTATACA GTTTGACATT 
ATTTCTCCTG TGTGTGAAGA TCAGTCTGGC CAGGCAAAAG CCTCTGGCCA GGGAGGCAGG 
CGTACAAATC CATTTGGAGA ATCTGGAGGA GGTACAAAGT CTGAAACTGA AGAGTCTATT 
CTTCATCAGT TGTTTATTGT CCGATTCCTT GGTTCTATGG AGGTGAAATC AGATGACAAT 
CCAGATGTTG TTTATGAAAC AATGCGCCAA ATTTTAGCTG CCCGGGCCAT CCATAACATC 
TTTCGTATGA CAGAATCACA TTTATTAGTC ACTTGTGATT GTTTAAAGTT AATTGATCCA 
CAGACACAAG TTACAAGGCT CACGTTTCCA TTACCCAGTG TAGTTCTGTA TGCTACACAT 
CAGGAAAATA AGAGGCTTTT TGGATTTGTT CTTCGGACAT CAGGAGGGAG AAGTGAAAGT 
AACTTGTCAT CAGTCTGTTA CATATTTGAA TCAAACAACG AGGGTGAAAA GATTTGTGAT 
TCTGTTGGAT TGGCAAAACA GATAGCATTG CATGCAGAAC TGGATCGCAG GGCATCAGAA 
AAACAAAAAG AAATAGAGAG AGTAAAAGAG AAGCAACAGA AAGAACTCAG TAAACAAAAA 
CAAATTGAGA AGGACTTAGA AGAACAAAGT CGGTTGATAG CTGCTTCCAG TAGACCAAAC 
CAAGCCAGTA GCGAGGGGCA GTTTGTTGTC CTTAGCAGTA GCCAGTCAGA AGAGAGTGAT 
TTGGGAGAAG AAGGAAAGAA GAGAGAGTCA GAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011955234.1
CDS	3433..5451
Translation	MNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNDQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVHRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQSGQAKASGQGGRRTNPFGESGGGTKSETEESILHQLFIVRFLGSMEVKSDDNPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPSVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_012099844.2
Organism	Ovis aries(sheep)
Definition	Ovis aries adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012099844.2

ORF Insert Sequence:

ATGAACCAGT TGTATCAGGC TATGCATCGG ATTTATGATG CACAGAATGA ATTAAGTGCA 
GCAACACACC TGACTTCAAA ACTTTTAAAA GAATATGAGA AACAGCGTTT TCCACTGGGG 
GGTGATGATG AAGTTATGAG TTCTACTTTG CAGCAGTTTT CAAAAGTTAT AGATGAGCTT 
AGCTCTTGTC ATGCGGTACT TTCAACACAG CTTGCTGATG CCATGATGTT CCCCATTACC 
CAGTTTAAAG AAAGAGATCT GAAAGAAATA TTGACATTAA AGGAAGTGTT TCAGATTGCT 
AGTAATGATC ATGATGCTGC AATTAACAGA TACAGCAGAT TGTCCAAAAA AAGAGAAAAT 
GACAAGGTGA AGTATGAAGT AACAGAAGAT GTCTACACTT CGAGAAAAAA ACAACACCAG 
ACCATGATGC ATTATTTTTG TGCGTTAAAT ACTCTTCAGT ACAAAAAGAA AATAGCATTG 
TTAGAACCTC TTCTGGGATA TATGCAAGCT CAGATAAGTT TCTTTAAGAT GGGTTCTGAA 
AATCTCAATG ACCAACTGGA AGAATTCTTA ACTAATATTG GAACAAGTGT ACAGAATGTT 
CGCAGGGAAA TGGACAGCGA TGTAGAGACC ATGCAGCAGA CAATAGAGGA CTTGGAAGTG 
GCCAGTGACC CCTTATATGT GCCTGACCCG GATCCCACAA AGTTTCCTGT TCATCGAAAC 
CTGACCCGGA AGGCGGGGTA CCTTAATGCT AGGAATAAAA CAGGCTTGGT GTCATCTACC 
TGGGACAGAC AGTTTTACTT CACGCAGGGT GGAAACCTGA TGAGTCAGGC CCGTGGCGAC 
GTAGCAGGAG GCCTGGCCAT GGACATAGAC AACTGCTCAG TCATGGCTGT GGACTGTGAA 
GACAGGCGGT ACTGTTTTCA GATCACTTCT TTCGACGGCA AAAAGTCTTC AATTTTGCAA 
GCAGAGAGTA AAAAAGACCA TGAAGAGTGG ATCTGTACAA TAAATAACAT ATCTAAACAA 
ATATATTTAA GTGAAAACCC AGAGGAGATT GCTGCACGAG TAAATCAGTC AGCTTTGGAA 
GCTGTCACTC CTTCCCCATC TTTCCAGCAG AGGCACGAGA GCCTGAGGCC GGCAGGACAA 
TCTCGGCCAC CCACAGCTCG AACCAGCAGT TCAGGATCCT TAGGATCTGA GTCCACAAAT 
TTGGCTGCCC TCTCTCTAGA TTCTCTTGTT GCCCCAGATA CCCCTATACA GTTTGACATT 
ATTTCTCCTG TGTGTGAAGA TCAGTCTGGC CAGGCAAAAG CCTCTGGCCA GGGAGGCAGG 
CGTACAAATC CATTTGGAGA ATCTGGAGGA GGTACAAAGT CTGAAACTGA AGAGTCTATT 
CTTCATCAGT TGTTTATTGT CCGATTCCTT GGTTCTATGG AGGTGAAATC AGATGACAAT 
CCAGATGTTG TTTATGAAAC AATGCGCCAA ATTTTAGCTG CCCGGGCCAT CCATAACATC 
TTTCGTATGA CAGAATCACA TTTATTAGTC ACTTGTGATT GTTTAAAGTT AATTGATCCA 
CAGACACAAG TTACAAGGCT CACGTTTCCA TTACCCAGTG TAGTTCTGTA TGCTACACAT 
CAGGAAAATA AGAGGCTTTT TGGATTTGTT CTTCGGACAT CAGGAGGGAG AAGTGAAAGT 
AACTTGTCAT CAGTCTGTTA CATATTTGAA TCAAACAACG AGGGTGAAAA GATTTGTGAT 
TCTGTTGGAT TGGCAAAACA GATAGCATTG CATGCAGAAC TGGATCGCAG GGCATCAGAA 
AAACAAAAAG AAATAGAGAG AGTAAAAGAG AAGCAACAGA AAGAACTCAG TAAACAAAAA 
CAAATTGAGA AGGACTTAGA AGAACAAAGT CGGTTGATAG CTGCTTCCAG TAGACCAAAC 
CAAGCCAGTA GCGAGGGGCA GTTTGTTGTC CTTAGCAGTA GCCAGTCAGA AGAGAGTGAT 
TTGGGAGAAG AAGGAAAGAA GAGAGAGTCA GAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_012126241.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011981631.1 Ovis aries musimon adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv11185
Clone ID Related Accession (Same CDS sequence)	XM_012126241.2
Accession Version	XM_012126241.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	DCC-interacting protein 13-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012126241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC ACAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCC ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAGGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACACCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAGAAA CAGCGTTTTC CACTGGGGGG TGATGATGAA 
GTTATGAGTT CTACTTTGCA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCGGTACTTT CAACACAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATATT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGATCAT 
GATGCTGCAA TTAACAGATA CAGCAGATTG TCCAAAAAAA GAGAAAATGA CAAGGTGAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCG AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CGTTAAATAC TCTTCAGTAC AAAAAGAAAA TAGCATTGTT AGAACCTCTT 
CTGGGATATA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTCAATGAC 
CAACTGGAAG AATTCTTAAC TAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGCGATG TAGAGACCAT GCAGCAGACA ATAGAGGACT TGGAAGTGGC CAGTGACCCC 
TTATATGTGC CTGACCCGGA TCCCACAAAG TTTCCTGTTC ATCGAAACCT GACCCGGAAG 
GCGGGGTACC TTAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGACAGACAG 
TTTTACTTCA CGCAGGGTGG AAACCTGATG AGTCAGGCCC GTGGCGACGT AGCAGGAGGC 
CTGGCCATGG ACATAGACAA CTGCTCAGTC ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCTTT CGACGGCAAA AAGTCTTCAA TTTTGCAAGC AGAGAGTAAA 
AAAGACCATG AAGAGTGGAT CTGTACAATA AATAACATAT CTAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAGATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG GCACGAGAGC CTGAGGCCGG CAGGACAATC TCGGCCACCC 
ACAGCTCGAA CCAGCAGTTC AGGATCCTTA GGATCTGAGT CCACAAATTT GGCTGCCCTC 
TCTCTAGATT CTCTTGTTGC CCCAGATACC CCTATACAGT TTGACATTAT TTCTCCTGTG 
TGTGAAGATC AGTCTGGCCA GGCAAAAGCC TCTGGCCAGG GAGGCAGGCG TACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAGTCT GAAACTGAAG AGTCTATTCT TCATCAGTTG 
TTTATTGTCC GATTCCTTGG TTCTATGGAG GTGAAATCAG ATGACAATCC AGATGTTGTT 
TATGAAACAA TGCGCCAAAT TTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAAGTT 
ACAAGGCTCA CGTTTCCATT ACCCAGTGTA GTTCTGTATG CTACACATCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA CTTGTCATCA 
GTCTGTTACA TATTTGAATC AAACAACGAG GGTGAAAAGA TTTGTGATTC TGTTGGATTG 
GCAAAACAGA TAGCATTGCA TGCAGAACTG GATCGCAGGG CATCAGAAAA ACAAAAAGAA 
ATAGAGAGAG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAGAAG 
GACTTAGAAG AACAAAGTCG GTTGATAGCT GCTTCCAGTA GACCAAACCA AGCCAGTAGC 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAGA GAGAGTCAGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011981631.1
CDS	111..2237
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNDQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVHRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQSGQAKASGQGGRRTNPFGESGGGTKSETEESILHQLFIVRFLGSMEVKSDDNPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPSVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_012126241.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012126241.2

ORF Insert Sequence:

ATGCCGGGGA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC ACAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCC ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAGGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACACCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAGAAA CAGCGTTTTC CACTGGGGGG TGATGATGAA 
GTTATGAGTT CTACTTTGCA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCGGTACTTT CAACACAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATATT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGATCAT 
GATGCTGCAA TTAACAGATA CAGCAGATTG TCCAAAAAAA GAGAAAATGA CAAGGTGAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCG AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CGTTAAATAC TCTTCAGTAC AAAAAGAAAA TAGCATTGTT AGAACCTCTT 
CTGGGATATA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTCAATGAC 
CAACTGGAAG AATTCTTAAC TAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGCGATG TAGAGACCAT GCAGCAGACA ATAGAGGACT TGGAAGTGGC CAGTGACCCC 
TTATATGTGC CTGACCCGGA TCCCACAAAG TTTCCTGTTC ATCGAAACCT GACCCGGAAG 
GCGGGGTACC TTAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGACAGACAG 
TTTTACTTCA CGCAGGGTGG AAACCTGATG AGTCAGGCCC GTGGCGACGT AGCAGGAGGC 
CTGGCCATGG ACATAGACAA CTGCTCAGTC ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCTTT CGACGGCAAA AAGTCTTCAA TTTTGCAAGC AGAGAGTAAA 
AAAGACCATG AAGAGTGGAT CTGTACAATA AATAACATAT CTAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAGATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG GCACGAGAGC CTGAGGCCGG CAGGACAATC TCGGCCACCC 
ACAGCTCGAA CCAGCAGTTC AGGATCCTTA GGATCTGAGT CCACAAATTT GGCTGCCCTC 
TCTCTAGATT CTCTTGTTGC CCCAGATACC CCTATACAGT TTGACATTAT TTCTCCTGTG 
TGTGAAGATC AGTCTGGCCA GGCAAAAGCC TCTGGCCAGG GAGGCAGGCG TACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAGTCT GAAACTGAAG AGTCTATTCT TCATCAGTTG 
TTTATTGTCC GATTCCTTGG TTCTATGGAG GTGAAATCAG ATGACAATCC AGATGTTGTT 
TATGAAACAA TGCGCCAAAT TTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAAGTT 
ACAAGGCTCA CGTTTCCATT ACCCAGTGTA GTTCTGTATG CTACACATCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA CTTGTCATCA 
GTCTGTTACA TATTTGAATC AAACAACGAG GGTGAAAAGA TTTGTGATTC TGTTGGATTG 
GCAAAACAGA TAGCATTGCA TGCAGAACTG GATCGCAGGG CATCAGAAAA ACAAAAAGAA 
ATAGAGAGAG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAGAAG 
GACTTAGAAG AACAAAGTCG GTTGATAGCT GCTTCCAGTA GACCAAACCA AGCCAGTAGC 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAGA GAGAGTCAGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_027958257.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027814058.1 Ovis aries adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), mRNA.

Price & Availability↑

CloneID	OSm69013
Clone ID Related Accession (Same CDS sequence)	XM_027958257.1
Accession Version	XM_027958257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	DCC-interacting protein 13-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040270.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC ACAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCC ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAGGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACACCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAGAAA CAGCGTTTTC CACTGGGGGG TGATGATGAA 
GTTATGAGTT CTACTTTGCA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCGGTACTTT CAACACAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATATT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGATCAT 
GATGCTGCAA TTAACAGATA CAGCAGATTG TCCAAAAAAA GAGAAAATGA CAAGGTGAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCG AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CGTTAAATAC TCTTCAGTAC AAAAAGAAAA TAGCATTGTT AGAACCTCTT 
CTGGGATATA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTCAATGAC 
CAACTGGAAG AATTCTTAAC TAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGCGATG TAGAGACCAT GCAGCAGACA ATAGAGGACT TGGAAGTGGC CAGTGACCCC 
TTATATGTGC CTGACCCGGA TCCCACAAAG TTTCCTGTTC ATCGAAACCT GACCCGGAAG 
GCGGGGTACC TTAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGACAGACAG 
TTTTACTTCA CGCAGGGTGG AAACCTGATG AGTCAGGCCC GTGGCGACGT AGCAGGAGGC 
CTGGCCATGG ACATAGACAA CTGCTCAGTC ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCTTT CGATGGCAAA AAGTCTTCAA TTTTGCAAGC AGAGAGTAAA 
AAAGACCATG AAGAGTGGAT CTGTACAATA AATAACATAT CTAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAGATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG GCACGAGAGC CTGAGGCCGG CAGGACAATC TCGGCCACCC 
ACAGCTCGAA CCAGCAGTTC AGGATCCTTA GGATCTGAGT CCACAAATTT GGCTGCCCTC 
TCTCTAGATT CTCTTGTTGC CCCAGATACC CCTATACAGT TTGACATTAT TTCTCCTGTG 
TGTGAAGATC AGTCTGGCCA GGCAAAAGCC TCTGGCCAGG GAGGCAGGCG TACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAGTCT GAAACTGAAG AGTCTATTCT TCATCAGTTG 
TTTATTGTCC GATTCCTTGG TTCTATGGAG GTGAAATCAG ATGACAATCC AGATGTTGTT 
TATGAAACAA TGCGCCAAAT TTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAAGTT 
ACAAGGCTCA CGTTTCCATT ACCCAGTGTA GTTCTGTATG CTACACATCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA CTTGTCATCA 
GTCTGTTACA TATTTGAATC AAACAACGAG GGTGAAAAGA TTTGTGATTC TGTTGGATTG 
GCAAAACAGA TAGCATTGCA TGCAGAACTG GATCGCAGGG CATCAGAAAA ACAAAAAGAA 
ATAGAGAGAG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAGAAG 
GACTTAGAAG AACAAAGTCG GTTGATAGCT GCTTCCAGTA GACCAAACCA AGCCAGTAGC 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAGA GAGAGTCAGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027814058.1
CDS	130..2256
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNDQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVHRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQSGQAKASGQGGRRTNPFGESGGGTKSETEESILHQLFIVRFLGSMEVKSDDNPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPSVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_027958257.1
Organism	Ovis aries(sheep)
Definition	Ovis aries adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027958257.1

ORF Insert Sequence:

ATGCCGGGGA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC ACAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCC ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAGGCTA TGCATCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACACCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAGAAA CAGCGTTTTC CACTGGGGGG TGATGATGAA 
GTTATGAGTT CTACTTTGCA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCGGTACTTT CAACACAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA GTTTAAAGAA 
AGAGATCTGA AAGAAATATT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGATCAT 
GATGCTGCAA TTAACAGATA CAGCAGATTG TCCAAAAAAA GAGAAAATGA CAAGGTGAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCG AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CGTTAAATAC TCTTCAGTAC AAAAAGAAAA TAGCATTGTT AGAACCTCTT 
CTGGGATATA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTCAATGAC 
CAACTGGAAG AATTCTTAAC TAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGCGATG TAGAGACCAT GCAGCAGACA ATAGAGGACT TGGAAGTGGC CAGTGACCCC 
TTATATGTGC CTGACCCGGA TCCCACAAAG TTTCCTGTTC ATCGAAACCT GACCCGGAAG 
GCGGGGTACC TTAATGCTAG GAATAAAACA GGCTTGGTGT CATCTACCTG GGACAGACAG 
TTTTACTTCA CGCAGGGTGG AAACCTGATG AGTCAGGCCC GTGGCGACGT AGCAGGAGGC 
CTGGCCATGG ACATAGACAA CTGCTCAGTC ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCTTT CGATGGCAAA AAGTCTTCAA TTTTGCAAGC AGAGAGTAAA 
AAAGACCATG AAGAGTGGAT CTGTACAATA AATAACATAT CTAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAGATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC TGTCACTCCT 
TCCCCATCTT TCCAGCAGAG GCACGAGAGC CTGAGGCCGG CAGGACAATC TCGGCCACCC 
ACAGCTCGAA CCAGCAGTTC AGGATCCTTA GGATCTGAGT CCACAAATTT GGCTGCCCTC 
TCTCTAGATT CTCTTGTTGC CCCAGATACC CCTATACAGT TTGACATTAT TTCTCCTGTG 
TGTGAAGATC AGTCTGGCCA GGCAAAAGCC TCTGGCCAGG GAGGCAGGCG TACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAGTCT GAAACTGAAG AGTCTATTCT TCATCAGTTG 
TTTATTGTCC GATTCCTTGG TTCTATGGAG GTGAAATCAG ATGACAATCC AGATGTTGTT 
TATGAAACAA TGCGCCAAAT TTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAAGTT 
ACAAGGCTCA CGTTTCCATT ACCCAGTGTA GTTCTGTATG CTACACATCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA CTTGTCATCA 
GTCTGTTACA TATTTGAATC AAACAACGAG GGTGAAAAGA TTTGTGATTC TGTTGGATTG 
GCAAAACAGA TAGCATTGCA TGCAGAACTG GATCGCAGGG CATCAGAAAA ACAAAAAGAA 
ATAGAGAGAG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAGAAG 
GACTTAGAAG AACAAAGTCG GTTGATAGCT GCTTCCAGTA GACCAAACCA AGCCAGTAGC 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAGA GAGAGTCAGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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APPL1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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