AGRN cDNA ORF clone, Ovis aries(sheep)

The following AGRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OOv18902 XM_012138576.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv18903 XM_012138578.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv18904 XM_012138579.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv18905 XM_012138580.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv18906 XM_012138581.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OSm61814 XM_027975848.1
Latest version!
Ovis aries agrin (AGRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OSm61815 XM_027975849.1
Latest version!
Ovis aries agrin (AGRN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OSm61817 XM_027975851.1
Latest version!
Ovis aries agrin (AGRN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OOv18898 XM_012138572.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OOv18899 XM_012138573.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OOv18900 XM_012138574.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OOv18901 XM_012138575.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OOv18907 XM_012138582.2
Latest version!
Ovis aries musimon agrin (AGRN), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSm51778 XM_015099069.1
Latest version!
Ovis aries agrin (AGRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSm61810 XM_027975844.1
Latest version!
Ovis aries agrin (AGRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OSm61811 XM_027975845.1
Latest version!
Ovis aries agrin (AGRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OSm61812 XM_027975846.1
Latest version!
Ovis aries agrin (AGRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OSm61813 XM_027975847.1
Latest version!
Ovis aries agrin (AGRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OSm61816 XM_027975850.1
Latest version!
Ovis aries agrin (AGRN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OOv18902
    Clone ID Related Accession (Same CDS sequence) XM_012138576.2
    Accession Version XM_012138576.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GGCACCCAGC AGCCCCGAAG GCCCTGTGAC
    TCCCAGCCCT GCCTCCATGG GGGGACCTGC CAGGACCAGG GCTCAGGTGC AGACTTCACC
    TGCAGCTGCC CAGCAGGCAC AGAGGGTGCC GTCTGTGAGA AGGCCCTGCA CCCCTCCGTG
    CCAGCCTTCG GGGGCTCCTC CTTCCTGGCC TTTCCCACCC TTCGTGCCTA CCACACCCTG
    CGCCTGGCGC TCGAGTTCCG GGCGCTGGAG CCCCAGGGGC TGCTGCTCTA CAACGGCAAT
    GCCCGGGGCA AGGACTTCCT GGGACTGGCG CTGCTGGGAG GCCGCGTGCA GTTCAGGTTC
    GACACAGGCT CAGGGCCCGC GGTGCTGACG AGCTCCGTGC CCGTGCAGCC CGGCCAGTGG
    CATCGCCTGG AGCTGTCTCG GCACTGGCGC CAAGGTACTC TGTCCGTGGA TGGTGAGACT
    CCTGTCCTGG GCCAGAGCCC CAGTGGCACT GATGGTCTCA ACCTGGACAC TGACCTTTTC
    GTGGGTGGCG TCCCTGAGGA TCAGGCTTCC ACGGTGCTAG AGCGGACCTC TGTCAGCGTC
    GGTCTTAGGG GTTGTATCCG CTTGCTGGAC GTCAACAATC AGCGGCTAGA GCTGAGCAGC
    TGGCCGGAAT CTGCGACTCG AAACTCCGGA GTGGGCGAGT GTGGGGACCA CCCCTGTCTG
    CCTAGCCCCT GCCTCGGAGG GGCCCCGTGC CAGGCCCTGG AGGCAGGCAG GTTCCGCTGT
    CAGTGCCCAC CCGGCCGCTT TGGCCCAACT TGTGCCGACG AGGACCCTTG TCAGCCGAAC
    CCCTGCCATG GAGCAGCCCC CTGCCGCGTG CTGCCTCAGG GAGAGGCCAA GTGCGAGTGC
    CCCCATGGCC GGGAGGGCAG CCTCTGCCAG ACAGTCTCAG AGCCAGAGGA CAACCAGCCG
    TTCCTGGCTG ATTTCAGCAG CTTCTCCTAC CTGGAACTGA AAGGCCTGCA CACCTTTGAG
    CGGGACCTGG GGGAGAAGAT GGCGCTGGAG GTGGTATTCC TGGCTCGGAG CCCCAGCGGC
    CTGCTCCTCT ATAATGGACA GAAGACAGAT GGCAAGGGTG ACTTTGTGTC TCTGGCGCTG
    CACAATGGCC TCCTGGAGTT CCGCTACGAC CTGGGCAAGG GGGCAGCGGT CATCAGGAGC
    AAGGAGCCGG TGGCCCTGGG TGCTTGGACC AGGGTCTCCC TGGAGCGAAA TGGACGCAAA
    GGAGCCATGC GGGTTGGCGA TGGGCCTCGC GTGCTGGGGG AGTCCCCGAA ATCCCGCAAG
    GTGCCACACA CCGTTCTCAA CCTAAAGGAG CCACTCTTTG TTGGGGGGGC CCCTGATTTC
    AGCAAGCTGG CCCGAGCAGC TGCCGTGTCC TCTGGCTTTG ATGGAGCCAT CCAGCTGGTC
    TCCCTGAATG GCCGCCAACT GCTGACCCGT GAGCATGTGG TCCGGGCCAT GGACGTCTCG
    TCCTTTGCAG ACCACCCTTG TACCCAGGCC GAGGGTCAGC CTTGCCTCCA TGGGGCTTCC
    TGTCTCCCCC GTGAGGCCTC CTATGAGTGC CTGTGTCCTG CAGGCTTCTC GGGGCTGCAC
    TGTGAGAAGG GCTTGATTGA GAAATCAGCT GGGGACCTGG ACGCACTGGC TTTTGATGGA
    CGGACCTACA TCGAGTACCT CAACGCTGTG ACCGAGAGCG AACTGACCAA TGAGATCCCA
    GCTGAGAAGG CACTCCAGAG CAACCACTTT GAGCTGAGCC TGCGCACTGA GGCCACACAG
    GGGCTGGTGC TGTGGAGTGG CAAGGCCACG GAGCGGGCTG ACTACATCGC ACTGGCCATC
    GTGGACGGGC GCCTGCAGCT GGCTTACGAC CTGGGCTCCC AGCCCGTGGT GCTGCGCTCC
    ACCGTACCAG TCAATACCAA CCGCTGGTTG CGGATCAGGG CACACAGGAA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA CGAGGCCCCT GTGACTGGCT CCTCCCCACT GGGTGCCACG
    CAGCTGGACA CAGATGGAGC CCTGTGGCTG GGTGGCCTGG GGAAGCTGCC CACGGGCCAG
    GCCCTGCCCA AAGCCTATGG CACAGGCTTT GTGGGCTGCC TGCGGGACGT GGTGGTGGGC
    CAGCGCCCGC TGCACCTGCT GGAGGACGCC ATCACCAAGC CAGAGCTTCG GCCCTGCCCT
    GCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993966.1
    CDS111..6059
    Translation

    Target ORF information:

    RefSeq Version XM_012138576.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138576.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GGCACCCAGC AGCCCCGAAG GCCCTGTGAC
    TCCCAGCCCT GCCTCCATGG GGGGACCTGC CAGGACCAGG GCTCAGGTGC AGACTTCACC
    TGCAGCTGCC CAGCAGGCAC AGAGGGTGCC GTCTGTGAGA AGGCCCTGCA CCCCTCCGTG
    CCAGCCTTCG GGGGCTCCTC CTTCCTGGCC TTTCCCACCC TTCGTGCCTA CCACACCCTG
    CGCCTGGCGC TCGAGTTCCG GGCGCTGGAG CCCCAGGGGC TGCTGCTCTA CAACGGCAAT
    GCCCGGGGCA AGGACTTCCT GGGACTGGCG CTGCTGGGAG GCCGCGTGCA GTTCAGGTTC
    GACACAGGCT CAGGGCCCGC GGTGCTGACG AGCTCCGTGC CCGTGCAGCC CGGCCAGTGG
    CATCGCCTGG AGCTGTCTCG GCACTGGCGC CAAGGTACTC TGTCCGTGGA TGGTGAGACT
    CCTGTCCTGG GCCAGAGCCC CAGTGGCACT GATGGTCTCA ACCTGGACAC TGACCTTTTC
    GTGGGTGGCG TCCCTGAGGA TCAGGCTTCC ACGGTGCTAG AGCGGACCTC TGTCAGCGTC
    GGTCTTAGGG GTTGTATCCG CTTGCTGGAC GTCAACAATC AGCGGCTAGA GCTGAGCAGC
    TGGCCGGAAT CTGCGACTCG AAACTCCGGA GTGGGCGAGT GTGGGGACCA CCCCTGTCTG
    CCTAGCCCCT GCCTCGGAGG GGCCCCGTGC CAGGCCCTGG AGGCAGGCAG GTTCCGCTGT
    CAGTGCCCAC CCGGCCGCTT TGGCCCAACT TGTGCCGACG AGGACCCTTG TCAGCCGAAC
    CCCTGCCATG GAGCAGCCCC CTGCCGCGTG CTGCCTCAGG GAGAGGCCAA GTGCGAGTGC
    CCCCATGGCC GGGAGGGCAG CCTCTGCCAG ACAGTCTCAG AGCCAGAGGA CAACCAGCCG
    TTCCTGGCTG ATTTCAGCAG CTTCTCCTAC CTGGAACTGA AAGGCCTGCA CACCTTTGAG
    CGGGACCTGG GGGAGAAGAT GGCGCTGGAG GTGGTATTCC TGGCTCGGAG CCCCAGCGGC
    CTGCTCCTCT ATAATGGACA GAAGACAGAT GGCAAGGGTG ACTTTGTGTC TCTGGCGCTG
    CACAATGGCC TCCTGGAGTT CCGCTACGAC CTGGGCAAGG GGGCAGCGGT CATCAGGAGC
    AAGGAGCCGG TGGCCCTGGG TGCTTGGACC AGGGTCTCCC TGGAGCGAAA TGGACGCAAA
    GGAGCCATGC GGGTTGGCGA TGGGCCTCGC GTGCTGGGGG AGTCCCCGAA ATCCCGCAAG
    GTGCCACACA CCGTTCTCAA CCTAAAGGAG CCACTCTTTG TTGGGGGGGC CCCTGATTTC
    AGCAAGCTGG CCCGAGCAGC TGCCGTGTCC TCTGGCTTTG ATGGAGCCAT CCAGCTGGTC
    TCCCTGAATG GCCGCCAACT GCTGACCCGT GAGCATGTGG TCCGGGCCAT GGACGTCTCG
    TCCTTTGCAG ACCACCCTTG TACCCAGGCC GAGGGTCAGC CTTGCCTCCA TGGGGCTTCC
    TGTCTCCCCC GTGAGGCCTC CTATGAGTGC CTGTGTCCTG CAGGCTTCTC GGGGCTGCAC
    TGTGAGAAGG GCTTGATTGA GAAATCAGCT GGGGACCTGG ACGCACTGGC TTTTGATGGA
    CGGACCTACA TCGAGTACCT CAACGCTGTG ACCGAGAGCG AACTGACCAA TGAGATCCCA
    GCTGAGAAGG CACTCCAGAG CAACCACTTT GAGCTGAGCC TGCGCACTGA GGCCACACAG
    GGGCTGGTGC TGTGGAGTGG CAAGGCCACG GAGCGGGCTG ACTACATCGC ACTGGCCATC
    GTGGACGGGC GCCTGCAGCT GGCTTACGAC CTGGGCTCCC AGCCCGTGGT GCTGCGCTCC
    ACCGTACCAG TCAATACCAA CCGCTGGTTG CGGATCAGGG CACACAGGAA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA CGAGGCCCCT GTGACTGGCT CCTCCCCACT GGGTGCCACG
    CAGCTGGACA CAGATGGAGC CCTGTGGCTG GGTGGCCTGG GGAAGCTGCC CACGGGCCAG
    GCCCTGCCCA AAGCCTATGG CACAGGCTTT GTGGGCTGCC TGCGGGACGT GGTGGTGGGC
    CAGCGCCCGC TGCACCTGCT GGAGGACGCC ATCACCAAGC CAGAGCTTCG GCCCTGCCCT
    GCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv18903
    Clone ID Related Accession (Same CDS sequence) XM_012138578.2
    Accession Version XM_012138578.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCGCTGTGG GCAGTGCCGC TTTGGAGCCC TGTGTGAAGC TGAGACTGGG
    CGCTGTGTGT GCCCCTCCGA ATGTGTGGCC TCAGCCCAGC CTGTGTGTGG CTCTGACGGG
    CGCACATATG CCAATGAATG TGAACTACAC GTCCACGCCT GCACACGTCA GATCAGTCTG
    CACGTGGCCT CAGCCAGTCC CTGCCAGACC TGTGGAGATA CAGTGTGTGC CTTTGGGGCT
    GTGTGCTCAG CGGGGCAGTG CGTGTGTCCC CGGTGTGAGC GCCCTCCACC CGGCCCCGTG
    TGTGGCAGTG ATGGCGTGAC CTACGGCAGT TCATGTGAGC TCCGTGAGGC TGCCTGTCAG
    CAGCAGACAC AGATCGAGGA AGCCCGGGTG GGGCCATGTG AGCAAGCTGA GTGTGGCTCC
    GGAGGCTCAG GTTCGGGAGA GGACGGCGAG TGTGAGCCTG AACTGTGCCG GCAGCGAGGT
    GGCATCTGGG ATGAGGACTC GGAGGATGGG CCATGCGTCT GCGGCTTCAG CTGCCAGGGC
    GTCCTGAGGA GCCCGGTGTG TGGCTCGGAT GGAGTCACCT ACCGCACCGA ATGTGAGCTG
    AAGAAGGCCA GGTGTGAATC ACAGCCAGAG CTGTATGTCG TGGCCCAGGG AGCCTGCCGT
    GGCCCCACTT TGGCCCCATT GCCACCTGCG ATTCCCCTGC ACTGTGCTCA GACCCCCTAC
    GGTTGCTGCC AGGACAACAT CACTGTTGCC CAGGGTGTGG GCCTGGCTGG CTGCCCCAGC
    TCATGCCAGT GTAACCCACA CGGCTCCTAT GGCGGCACCT GTGACCCAGT CACAGGCCAG
    TGCTCCTGCC GCCCGGGTGT GGGGGGCCTC AAGTGTGACC GCTGTGAGCC TGGCTTCTGG
    AACTTCCGTG GTATCGTCAC TGATGGCCGG AGCGGCTGTA CCCCCTGCAG CTGTGACCCC
    CGGGGCGCCG TGCGGGACGA CTGTGAGCAG ATGACTGGGC TATGCTCCTG TAAGCCTGGG
    GTGGCCGGGC CCAAGTGTGG GCAGTGTCCA GATGGCCGTG CTCTGGGCCC TGCTGGCTGT
    GAAACAGACT CTTCGCAACC CACGACTTGT GCTGAGATGC TTTGTGAGTT TGGGGCATCC
    TGTGTGGAGG AGGCTGGCTC TGCCCACTGC GTGTGCCCAA CACCCACCTG TCCAGCGGCT
    GATGCTACCA AGGTCTGTGG GTCAGATGGA GTCACTTATG GCAATGAGTG CCAGCTGAGG
    ACCATCGCCT GCCGCCAGGG CTTGGAGATC TCTATCCAGA GCTTCGGCCC CTGCCAGGAA
    GGCATCACTT CTGGTCCGCA TCAGACATCC GCATCTGTGG CCCCCTTAGC ACTTGACCTG
    AACAAGGCAC CATTGCTGCC CCCCAGCGCC TTGCCCCTGG CTCCCAGCAG TACCCGCCAC
    AGCCAGCCCA CTGCTCGAGC CTCATCACAA CCTTGGACCA TGGCCAGCAT CCCCAGGACC
    ACCGCAAGGC CAGTGCTGAC CATGCCTCCC ATGGCCCCCT CCCCGGCAGC CAGCCTCGTC
    ACCTCTGCCT TCGGCGAATC TGGCAGCGCT GATGGGAGCG GTGACGAGGA GCTGAGCGGT
    GACTTGGAGG CCAGTGGGAC TGGCTCAGGA GGACTTGAGT CCCCGGAAAG AGACAGTGCT
    GGGACCCCTG GGCTACCCAT GGAAAGGGCT TCTTGCTACA ACTCACCTAT GGGCTGCTGC
    TCAGATGGCA AGACACCCTC CCTGGACGCA GAGGGCTCTA ACTGTCCTGC CACCAAGGCG
    TTCCAGGGTG TGCTGGAGCT CGAAGGGGTC GAGGGGCAAG AACTGTTCTA CACGCCGGAG
    ATGGCGGACC CCAAGTCTGA GCTGTTTGGG GAGACTGCCA GGAGCATTGA GAGCGCACTG
    GATGACCTCT TCCGGAATTC AGACGTTAAG AAGGACTTCC GAAGTGTCCG CCTACGGGAC
    TTGGGGCCAG GCAGCTCGGT CCGGGCCATT GTGGAAGTGC ACTTTGACCC CACCACAGCC
    TTCAGAGCGT CCGACGTGGG CCAGGCCCTG CTCCGGCAGC TCCAGGTGTC CAGGCGCAGA
    TCCCTGGGCG TGAAGCGGCC TCTGCAGGAA CACGTGCGGT TCATGGACTT CGACTGGTTT
    CCTGCATTCT TCACGGGAGC CACCCCAGCT GCAGCCACAG CCAGAGCCAC CACGGTGGCC
    CGCCTGCCAC CTTCAGCTGC AACCCCTCGG GCCCACTTTC CCAGTCACAC AAGCCGGCCT
    GTCAGCAGGA CCACACCTCC CCTGACCACG CGCCAGCCCC CCACCACTGC CCCTAGTCGA
    GTGCCTGGAC GCCGTCCCCT GCCCCCAGGC ACCCAGCAGC CCCGAAGGCC CTGTGACTCC
    CAGCCCTGCC TCCATGGGGG GACCTGCCAG GACCAGGGCT CAGGTGCAGA CTTCACCTGC
    AGCTGCCCAG CAGGCACAGA GGGTGCCGTC TGTGAGAAGG CCCTGCACCC CTCCGTGCCA
    GCCTTCGGGG GCTCCTCCTT CCTGGCCTTT CCCACCCTTC GTGCCTACCA CACCCTGCGC
    CTGGCGCTCG AGTTCCGGGC GCTGGAGCCC CAGGGGCTGC TGCTCTACAA CGGCAATGCC
    CGGGGCAAGG ACTTCCTGGG ACTGGCGCTG CTGGGAGGCC GCGTGCAGTT CAGGTTCGAC
    ACAGGCTCAG GGCCCGCGGT GCTGACGAGC TCCGTGCCCG TGCAGCCCGG CCAGTGGCAT
    CGCCTGGAGC TGTCTCGGCA CTGGCGCCAA GGTACTCTGT CCGTGGATGG TGAGACTCCT
    GTCCTGGGCC AGAGCCCCAG TGGCACTGAT GGTCTCAACC TGGACACTGA CCTTTTCGTG
    GGTGGCGTCC CTGAGGATCA GGCTTCCACG GTGCTAGAGC GGACCTCTGT CAGCGTCGGT
    CTTAGGGGTT GTATCCGCTT GCTGGACGTC AACAATCAGC GGCTAGAGCT GAGCAGCTGG
    CCGGAATCTG CGACTCGAAA CTCCGGAGTG GGCGAGTGTG GGGACCACCC CTGTCTGCCT
    AGCCCCTGCC TCGGAGGGGC CCCGTGCCAG GCCCTGGAGG CAGGCAGGTT CCGCTGTCAG
    TGCCCACCCG GCCGCTTTGG CCCAACTTGT GCCGACGAGG ACCCTTGTCA GCCGAACCCC
    TGCCATGGAG CAGCCCCCTG CCGCGTGCTG CCTCAGGGAG AGGCCAAGTG CGAGTGCCCC
    CATGGCCGGG AGGGCAGCCT CTGCCAGACA GTCTCAGAGC CAGAGGACAA CCAGCCGTTC
    CTGGCTGATT TCAGCAGCTT CTCCTACCTG GAACTGAAAG GCCTGCACAC CTTTGAGCGG
    GACCTGGGGG AGAAGATGGC GCTGGAGGTG GTATTCCTGG CTCGGAGCCC CAGCGGCCTG
    CTCCTCTATA ATGGACAGAA GACAGATGGC AAGGGTGACT TTGTGTCTCT GGCGCTGCAC
    AATGGCCTCC TGGAGTTCCG CTACGACCTG GGCAAGGGGG CAGCGGTCAT CAGGAGCAAG
    GAGCCGGTGG CCCTGGGTGC TTGGACCAGG GTCTCCCTGG AGCGAAATGG ACGCAAAGGA
    GCCATGCGGG TTGGCGATGG GCCTCGCGTG CTGGGGGAGT CCCCGAAATC CCGCAAGGTG
    CCACACACCG TTCTCAACCT AAAGGAGCCA CTCTTTGTTG GGGGGGCCCC TGATTTCAGC
    AAGCTGGCCC GAGCAGCTGC CGTGTCCTCT GGCTTTGATG GAGCCATCCA GCTGGTCTCC
    CTGAATGGCC GCCAACTGCT GACCCGTGAG CATGTGGTCC GGGCCATGGA CGTCTCGTCC
    TTTGCAGACC ACCCTTGTAC CCAGGCCGAG GGTCAGCCTT GCCTCCATGG GGCTTCCTGT
    CTCCCCCGTG AGGCCTCCTA TGAGTGCCTG TGTCCTGCAG GCTTCTCGGG GCTGCACTGT
    GAGAAGGGCT TGATTGAGAA ATCAGCTGGG GACCTGGACG CACTGGCTTT TGATGGACGG
    ACCTACATCG AGTACCTCAA CGCTGTGACC GAGAGCGAAC TGACCAATGA GATCCCAGCT
    GAGAAGGCAC TCCAGAGCAA CCACTTTGAG CTGAGCCTGC GCACTGAGGC CACACAGGGG
    CTGGTGCTGT GGAGTGGCAA GGCCACGGAG CGGGCTGACT ACATCGCACT GGCCATCGTG
    GACGGGCGCC TGCAGCTGGC TTACGACCTG GGCTCCCAGC CCGTGGTGCT GCGCTCCACC
    GTACCAGTCA ATACCAACCG CTGGTTGCGG ATCAGGGCAC ACAGGAAACA GAGGGAAGGT
    TCCCTTCAGG TGGGCAACGA GGCCCCTGTG ACTGGCTCCT CCCCACTGGG TGCCACGCAG
    CTGGACACAG ATGGAGCCCT GTGGCTGGGT GGCCTGGGGA AGCTGCCCAC GGGCCAGGCC
    CTGCCCAAAG CCTATGGCAC AGGCTTTGTG GGCTGCCTGC GGGACGTGGT GGTGGGCCAG
    CGCCCGCTGC ACCTGCTGGA GGACGCCATC ACCAAGCCAG AGCTTCGGCC CTGCCCTGCC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993968.1
    CDS111..6056
    Translation

    Target ORF information:

    RefSeq Version XM_012138578.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138578.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCGCTGTGG GCAGTGCCGC TTTGGAGCCC TGTGTGAAGC TGAGACTGGG
    CGCTGTGTGT GCCCCTCCGA ATGTGTGGCC TCAGCCCAGC CTGTGTGTGG CTCTGACGGG
    CGCACATATG CCAATGAATG TGAACTACAC GTCCACGCCT GCACACGTCA GATCAGTCTG
    CACGTGGCCT CAGCCAGTCC CTGCCAGACC TGTGGAGATA CAGTGTGTGC CTTTGGGGCT
    GTGTGCTCAG CGGGGCAGTG CGTGTGTCCC CGGTGTGAGC GCCCTCCACC CGGCCCCGTG
    TGTGGCAGTG ATGGCGTGAC CTACGGCAGT TCATGTGAGC TCCGTGAGGC TGCCTGTCAG
    CAGCAGACAC AGATCGAGGA AGCCCGGGTG GGGCCATGTG AGCAAGCTGA GTGTGGCTCC
    GGAGGCTCAG GTTCGGGAGA GGACGGCGAG TGTGAGCCTG AACTGTGCCG GCAGCGAGGT
    GGCATCTGGG ATGAGGACTC GGAGGATGGG CCATGCGTCT GCGGCTTCAG CTGCCAGGGC
    GTCCTGAGGA GCCCGGTGTG TGGCTCGGAT GGAGTCACCT ACCGCACCGA ATGTGAGCTG
    AAGAAGGCCA GGTGTGAATC ACAGCCAGAG CTGTATGTCG TGGCCCAGGG AGCCTGCCGT
    GGCCCCACTT TGGCCCCATT GCCACCTGCG ATTCCCCTGC ACTGTGCTCA GACCCCCTAC
    GGTTGCTGCC AGGACAACAT CACTGTTGCC CAGGGTGTGG GCCTGGCTGG CTGCCCCAGC
    TCATGCCAGT GTAACCCACA CGGCTCCTAT GGCGGCACCT GTGACCCAGT CACAGGCCAG
    TGCTCCTGCC GCCCGGGTGT GGGGGGCCTC AAGTGTGACC GCTGTGAGCC TGGCTTCTGG
    AACTTCCGTG GTATCGTCAC TGATGGCCGG AGCGGCTGTA CCCCCTGCAG CTGTGACCCC
    CGGGGCGCCG TGCGGGACGA CTGTGAGCAG ATGACTGGGC TATGCTCCTG TAAGCCTGGG
    GTGGCCGGGC CCAAGTGTGG GCAGTGTCCA GATGGCCGTG CTCTGGGCCC TGCTGGCTGT
    GAAACAGACT CTTCGCAACC CACGACTTGT GCTGAGATGC TTTGTGAGTT TGGGGCATCC
    TGTGTGGAGG AGGCTGGCTC TGCCCACTGC GTGTGCCCAA CACCCACCTG TCCAGCGGCT
    GATGCTACCA AGGTCTGTGG GTCAGATGGA GTCACTTATG GCAATGAGTG CCAGCTGAGG
    ACCATCGCCT GCCGCCAGGG CTTGGAGATC TCTATCCAGA GCTTCGGCCC CTGCCAGGAA
    GGCATCACTT CTGGTCCGCA TCAGACATCC GCATCTGTGG CCCCCTTAGC ACTTGACCTG
    AACAAGGCAC CATTGCTGCC CCCCAGCGCC TTGCCCCTGG CTCCCAGCAG TACCCGCCAC
    AGCCAGCCCA CTGCTCGAGC CTCATCACAA CCTTGGACCA TGGCCAGCAT CCCCAGGACC
    ACCGCAAGGC CAGTGCTGAC CATGCCTCCC ATGGCCCCCT CCCCGGCAGC CAGCCTCGTC
    ACCTCTGCCT TCGGCGAATC TGGCAGCGCT GATGGGAGCG GTGACGAGGA GCTGAGCGGT
    GACTTGGAGG CCAGTGGGAC TGGCTCAGGA GGACTTGAGT CCCCGGAAAG AGACAGTGCT
    GGGACCCCTG GGCTACCCAT GGAAAGGGCT TCTTGCTACA ACTCACCTAT GGGCTGCTGC
    TCAGATGGCA AGACACCCTC CCTGGACGCA GAGGGCTCTA ACTGTCCTGC CACCAAGGCG
    TTCCAGGGTG TGCTGGAGCT CGAAGGGGTC GAGGGGCAAG AACTGTTCTA CACGCCGGAG
    ATGGCGGACC CCAAGTCTGA GCTGTTTGGG GAGACTGCCA GGAGCATTGA GAGCGCACTG
    GATGACCTCT TCCGGAATTC AGACGTTAAG AAGGACTTCC GAAGTGTCCG CCTACGGGAC
    TTGGGGCCAG GCAGCTCGGT CCGGGCCATT GTGGAAGTGC ACTTTGACCC CACCACAGCC
    TTCAGAGCGT CCGACGTGGG CCAGGCCCTG CTCCGGCAGC TCCAGGTGTC CAGGCGCAGA
    TCCCTGGGCG TGAAGCGGCC TCTGCAGGAA CACGTGCGGT TCATGGACTT CGACTGGTTT
    CCTGCATTCT TCACGGGAGC CACCCCAGCT GCAGCCACAG CCAGAGCCAC CACGGTGGCC
    CGCCTGCCAC CTTCAGCTGC AACCCCTCGG GCCCACTTTC CCAGTCACAC AAGCCGGCCT
    GTCAGCAGGA CCACACCTCC CCTGACCACG CGCCAGCCCC CCACCACTGC CCCTAGTCGA
    GTGCCTGGAC GCCGTCCCCT GCCCCCAGGC ACCCAGCAGC CCCGAAGGCC CTGTGACTCC
    CAGCCCTGCC TCCATGGGGG GACCTGCCAG GACCAGGGCT CAGGTGCAGA CTTCACCTGC
    AGCTGCCCAG CAGGCACAGA GGGTGCCGTC TGTGAGAAGG CCCTGCACCC CTCCGTGCCA
    GCCTTCGGGG GCTCCTCCTT CCTGGCCTTT CCCACCCTTC GTGCCTACCA CACCCTGCGC
    CTGGCGCTCG AGTTCCGGGC GCTGGAGCCC CAGGGGCTGC TGCTCTACAA CGGCAATGCC
    CGGGGCAAGG ACTTCCTGGG ACTGGCGCTG CTGGGAGGCC GCGTGCAGTT CAGGTTCGAC
    ACAGGCTCAG GGCCCGCGGT GCTGACGAGC TCCGTGCCCG TGCAGCCCGG CCAGTGGCAT
    CGCCTGGAGC TGTCTCGGCA CTGGCGCCAA GGTACTCTGT CCGTGGATGG TGAGACTCCT
    GTCCTGGGCC AGAGCCCCAG TGGCACTGAT GGTCTCAACC TGGACACTGA CCTTTTCGTG
    GGTGGCGTCC CTGAGGATCA GGCTTCCACG GTGCTAGAGC GGACCTCTGT CAGCGTCGGT
    CTTAGGGGTT GTATCCGCTT GCTGGACGTC AACAATCAGC GGCTAGAGCT GAGCAGCTGG
    CCGGAATCTG CGACTCGAAA CTCCGGAGTG GGCGAGTGTG GGGACCACCC CTGTCTGCCT
    AGCCCCTGCC TCGGAGGGGC CCCGTGCCAG GCCCTGGAGG CAGGCAGGTT CCGCTGTCAG
    TGCCCACCCG GCCGCTTTGG CCCAACTTGT GCCGACGAGG ACCCTTGTCA GCCGAACCCC
    TGCCATGGAG CAGCCCCCTG CCGCGTGCTG CCTCAGGGAG AGGCCAAGTG CGAGTGCCCC
    CATGGCCGGG AGGGCAGCCT CTGCCAGACA GTCTCAGAGC CAGAGGACAA CCAGCCGTTC
    CTGGCTGATT TCAGCAGCTT CTCCTACCTG GAACTGAAAG GCCTGCACAC CTTTGAGCGG
    GACCTGGGGG AGAAGATGGC GCTGGAGGTG GTATTCCTGG CTCGGAGCCC CAGCGGCCTG
    CTCCTCTATA ATGGACAGAA GACAGATGGC AAGGGTGACT TTGTGTCTCT GGCGCTGCAC
    AATGGCCTCC TGGAGTTCCG CTACGACCTG GGCAAGGGGG CAGCGGTCAT CAGGAGCAAG
    GAGCCGGTGG CCCTGGGTGC TTGGACCAGG GTCTCCCTGG AGCGAAATGG ACGCAAAGGA
    GCCATGCGGG TTGGCGATGG GCCTCGCGTG CTGGGGGAGT CCCCGAAATC CCGCAAGGTG
    CCACACACCG TTCTCAACCT AAAGGAGCCA CTCTTTGTTG GGGGGGCCCC TGATTTCAGC
    AAGCTGGCCC GAGCAGCTGC CGTGTCCTCT GGCTTTGATG GAGCCATCCA GCTGGTCTCC
    CTGAATGGCC GCCAACTGCT GACCCGTGAG CATGTGGTCC GGGCCATGGA CGTCTCGTCC
    TTTGCAGACC ACCCTTGTAC CCAGGCCGAG GGTCAGCCTT GCCTCCATGG GGCTTCCTGT
    CTCCCCCGTG AGGCCTCCTA TGAGTGCCTG TGTCCTGCAG GCTTCTCGGG GCTGCACTGT
    GAGAAGGGCT TGATTGAGAA ATCAGCTGGG GACCTGGACG CACTGGCTTT TGATGGACGG
    ACCTACATCG AGTACCTCAA CGCTGTGACC GAGAGCGAAC TGACCAATGA GATCCCAGCT
    GAGAAGGCAC TCCAGAGCAA CCACTTTGAG CTGAGCCTGC GCACTGAGGC CACACAGGGG
    CTGGTGCTGT GGAGTGGCAA GGCCACGGAG CGGGCTGACT ACATCGCACT GGCCATCGTG
    GACGGGCGCC TGCAGCTGGC TTACGACCTG GGCTCCCAGC CCGTGGTGCT GCGCTCCACC
    GTACCAGTCA ATACCAACCG CTGGTTGCGG ATCAGGGCAC ACAGGAAACA GAGGGAAGGT
    TCCCTTCAGG TGGGCAACGA GGCCCCTGTG ACTGGCTCCT CCCCACTGGG TGCCACGCAG
    CTGGACACAG ATGGAGCCCT GTGGCTGGGT GGCCTGGGGA AGCTGCCCAC GGGCCAGGCC
    CTGCCCAAAG CCTATGGCAC AGGCTTTGTG GGCTGCCTGC GGGACGTGGT GGTGGGCCAG
    CGCCCGCTGC ACCTGCTGGA GGACGCCATC ACCAAGCCAG AGCTTCGGCC CTGCCCTGCC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv18904
    Clone ID Related Accession (Same CDS sequence) XM_012138579.2
    Accession Version XM_012138579.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC GCAGGGTCTC CGTCCTGGTG 
    CGGTATTTCA TGATCCCCTG TAATGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC
    TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC
    CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TTGGCGCTGT CTGCGAGCCC
    AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCGCCTG CCCCAGCGTG
    GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGAG GCGCATCCGC CTGCTGCGCC GCGGGCCCTG CGGGACCCGG
    GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCCTC TGCGGATGGA
    CTGACAGCTA CGTGCTTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAACG CCCTGTGTGC
    GGCAGCGACG GCAGTGACTA CCCCAGTGAG TGCCAGCTCT TGCGCCAAGC CTGTGCCCAC
    CAAGAGAACG TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTCCCTCAGT
    GACCTGAGCC GTACCTGCCG TGTGAACCCG CGCACGCGGC AGCCGGAGAT GCTCCTGCGA
    CCAGAGAGCT GCCCTCCCCG TGGGACACCC GTGTGTGGGG ACGACGGGGT TACTTACGAT
    AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCGAGGCC TCCTCCTGCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC
    CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGTGACCGCG TCGTCTGTGA CGGGGCCTAC
    AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC
    GAGTGTCAGC AGCAACGTAG CATCCCCATG AAGCACCAGG GCCCGTGTGA CCAGCCCCCA
    TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCGACGT GTACTGTGAA GAATGGGGAA
    GCCGAGTGTG CATGTCACCA GGTGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT
    GGCGTCACGT ATGGCAGTGT GTGCGAACTG GAAGCTTCAG CCTGTGCCCT TAGGCGGGAG
    ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG
    TGTGAAGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT
    GTGTGTGGCT CTGACGGGCG CACATATGCC AATGAATGTG AACTACACGT CCACGCCTGC
    ACACGTCAGA TCAGTCTGCA CGTGGCCTCA GCCAGTCCCT GCCAGACCTG TGGAGATACA
    GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC
    CCTCCACCCG GCCCCGTGTG TGGCAGTGAT GGCGTGACCT ACGGCAGTTC ATGTGAGCTC
    CGTGAGGCTG CCTGTCAGCA GCAGACACAG ATCGAGGAAG CCCGGGTGGG GCCATGTGAG
    CAAGCTGAGT GTGGCTCCGG AGGCTCAGGT TCGGGAGAGG ACGGCGAGTG TGAGCCTGAA
    CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGATGGGCC ATGCGTCTGC
    GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC
    CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG
    GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCATTGC CACCTGCGAT TCCCCTGCAC
    TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTATGG CGGCACCTGT
    GACCCAGTCA CAGGCCAGTG CTCCTGCCGC CCGGGTGTGG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ATGGCCGGAG CGGCTGTACC
    CCCTGCAGCT GTGACCCCCG GGGCGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA
    TGCTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCT
    CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCAACCCA CGACTTGTGC TGAGATGCTT
    TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA
    CCCACCTGTC CAGCGGCTGA TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACTTATGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAGAGC
    TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCGCATC AGACATCCGC ATCTGTGGCC
    CCCTTAGCAC TTGACCTGAA CAAGGCACCA TTGCTGCCCC CCAGCGCCTT GCCCCTGGCT
    CCCAGCAGTA CCCGCCACAG CCAGCCCACT GCTCGAGCCT CATCACAACC TTGGACCATG
    GCCAGCATCC CCAGGACCAC CGCAAGGCCA GTGCTGACCA TGCCTCCCAT GGCCCCCTCC
    CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGCGGT
    GACGAGGAGC TGAGCGGTGA CTTGGAGGCC AGTGGGACTG GCTCAGGAGG ACTTGAGTCC
    CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC TTGCTACAAC
    TCACCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG CTGGAGCTCG AAGGGGTCGA GGGGCAAGAA
    CTGTTCTACA CGCCGGAGAT GGCGGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG
    AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAATTCAG ACGTTAAGAA GGACTTCCGA
    AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC
    TTTGACCCCA CCACAGCCTT CAGAGCGTCC GACGTGGGCC AGGCCCTGCT CCGGCAGCTC
    CAGGTGTCCA GGCGCAGATC CCTGGGCGTG AAGCGGCCTC TGCAGGAACA CGTGCGGTTC
    ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACAGCC
    AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC
    AGTCACACAA GCCGGCCTGT CAGCAGGACC ACACCTCCCC TGACCACGCG CCAGCCCCCC
    ACCACTGCCC CTAGTCGAGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC
    CGAAGGCCCT GTGACTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCA
    GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACAGAGG GTGCCGTCTG TGAGAAGGCC
    CTGCACCCCT CCGTGCCAGC CTTCGGGGGC TCCTCCTTCC TGGCCTTTCC CACCCTTCGT
    GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG
    CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGCGCTGCT GGGAGGCCGC
    GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACGAGCTC CGTGCCCGTG
    CAGCCCGGCC AGTGGCATCG CCTGGAGCTG TCTCGGCACT GGCGCCAAGG TACTCTGTCC
    GTGGATGGTG AGACTCCTGT CCTGGGCCAG AGCCCCAGTG GCACTGATGG TCTCAACCTG
    GACACTGACC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG
    ACCTCTGTCA GCGTCGGTCT TAGGGGTTGT ATCCGCTTGC TGGACGTCAA CAATCAGCGG
    CTAGAGCTGA GCAGCTGGCC GGAATCTGCG ACTCGAAACT CCGGAGTGGG CGAGTGTGGG
    GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA
    GGCAGGTTCC GCTGTCAGTG CCCACCCGGC CGCTTTGGCC CAACTTGTGC CGACGAGGAC
    CCTTGTCAGC CGAACCCCTG CCATGGAGCA GCCCCCTGCC GCGTGCTGCC TCAGGGAGAG
    GCCAAGTGCG AGTGCCCCCA TGGCCGGGAG GGCAGCCTCT GCCAGACAGT CTCAGAGCCA
    GAGGACAACC AGCCGTTCCT GGCTGATTTC AGCAGCTTCT CCTACCTGGA ACTGAAAGGC
    CTGCACACCT TTGAGCGGGA CCTGGGGGAG AAGATGGCGC TGGAGGTGGT ATTCCTGGCT
    CGGAGCCCCA GCGGCCTGCT CCTCTATAAT GGACAGAAGA CAGATGGCAA GGGTGACTTT
    GTGTCTCTGG CGCTGCACAA TGGCCTCCTG GAGTTCCGCT ACGACCTGGG CAAGGGGGCA
    GCGGTCATCA GGAGCAAGGA GCCGGTGGCC CTGGGTGCTT GGACCAGGGT CTCCCTGGAG
    CGAAATGGAC GCAAAGGAGC CATGCGGGTT GGCGATGGGC CTCGCGTGCT GGGGGAGTCC
    CCGAAATCCC GCAAGGTGCC ACACACCGTT CTCAACCTAA AGGAGCCACT CTTTGTTGGG
    GGGGCCCCTG ATTTCAGCAA GCTGGCCCGA GCAGCTGCCG TGTCCTCTGG CTTTGATGGA
    GCCATCCAGC TGGTCTCCCT GAATGGCCGC CAACTGCTGA CCCGTGAGCA TGTGGTCCGG
    GCCATGGACG TCTCGTCCTT TGCAGACCAC CCTTGTACCC AGGCCGAGGG TCAGCCTTGC
    CTCCATGGGG CTTCCTGTCT CCCCCGTGAG GCCTCCTATG AGTGCCTGTG TCCTGCAGGC
    TTCTCGGGGC TGCACTGTGA GAAGGGCTTG ATTGAGAAAT CAGCTGGGGA CCTGGACGCA
    CTGGCTTTTG ATGGACGGAC CTACATCGAG TACCTCAACG CTGTGACCGA GAGCGAACTG
    ACCAATGAGA TCCCAGCTGA GAAGGCACTC CAGAGCAACC ACTTTGAGCT GAGCCTGCGC
    ACTGAGGCCA CACAGGGGCT GGTGCTGTGG AGTGGCAAGG CCACGGAGCG GGCTGACTAC
    ATCGCACTGG CCATCGTGGA CGGGCGCCTG CAGCTGGCTT ACGACCTGGG CTCCCAGCCC
    GTGGTGCTGC GCTCCACCGT ACCAGTCAAT ACCAACCGCT GGTTGCGGAT CAGGGCACAC
    AGGAAACAGA GGGAAGGTTC CCTTCAGGTG GGCAACGAGG CCCCTGTGAC TGGCTCCTCC
    CCACTGGGTG CCACGCAGCT GGACACAGAT GGAGCCCTGT GGCTGGGTGG CCTGGGGAAG
    CTGCCCACGG GCCAGGCCCT GCCCAAAGCC TATGGCACAG GCTTTGTGGG CTGCCTGCGG
    GACGTGGTGG TGGGCCAGCG CCCGCTGCAC CTGCTGGAGG ACGCCATCAC CAAGCCAGAG
    CTTCGGCCCT GCCCTGCCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993969.1
    CDS26..5869
    Translation

    Target ORF information:

    RefSeq Version XM_012138579.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138579.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC GCAGGGTCTC CGTCCTGGTG 
    CGGTATTTCA TGATCCCCTG TAATGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC
    TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC
    CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TTGGCGCTGT CTGCGAGCCC
    AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCGCCTG CCCCAGCGTG
    GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGAG GCGCATCCGC CTGCTGCGCC GCGGGCCCTG CGGGACCCGG
    GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCCTC TGCGGATGGA
    CTGACAGCTA CGTGCTTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAACG CCCTGTGTGC
    GGCAGCGACG GCAGTGACTA CCCCAGTGAG TGCCAGCTCT TGCGCCAAGC CTGTGCCCAC
    CAAGAGAACG TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTCCCTCAGT
    GACCTGAGCC GTACCTGCCG TGTGAACCCG CGCACGCGGC AGCCGGAGAT GCTCCTGCGA
    CCAGAGAGCT GCCCTCCCCG TGGGACACCC GTGTGTGGGG ACGACGGGGT TACTTACGAT
    AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCGAGGCC TCCTCCTGCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC
    CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGTGACCGCG TCGTCTGTGA CGGGGCCTAC
    AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC
    GAGTGTCAGC AGCAACGTAG CATCCCCATG AAGCACCAGG GCCCGTGTGA CCAGCCCCCA
    TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCGACGT GTACTGTGAA GAATGGGGAA
    GCCGAGTGTG CATGTCACCA GGTGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT
    GGCGTCACGT ATGGCAGTGT GTGCGAACTG GAAGCTTCAG CCTGTGCCCT TAGGCGGGAG
    ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG
    TGTGAAGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT
    GTGTGTGGCT CTGACGGGCG CACATATGCC AATGAATGTG AACTACACGT CCACGCCTGC
    ACACGTCAGA TCAGTCTGCA CGTGGCCTCA GCCAGTCCCT GCCAGACCTG TGGAGATACA
    GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC
    CCTCCACCCG GCCCCGTGTG TGGCAGTGAT GGCGTGACCT ACGGCAGTTC ATGTGAGCTC
    CGTGAGGCTG CCTGTCAGCA GCAGACACAG ATCGAGGAAG CCCGGGTGGG GCCATGTGAG
    CAAGCTGAGT GTGGCTCCGG AGGCTCAGGT TCGGGAGAGG ACGGCGAGTG TGAGCCTGAA
    CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGATGGGCC ATGCGTCTGC
    GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC
    CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG
    GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCATTGC CACCTGCGAT TCCCCTGCAC
    TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTATGG CGGCACCTGT
    GACCCAGTCA CAGGCCAGTG CTCCTGCCGC CCGGGTGTGG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ATGGCCGGAG CGGCTGTACC
    CCCTGCAGCT GTGACCCCCG GGGCGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA
    TGCTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCT
    CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCAACCCA CGACTTGTGC TGAGATGCTT
    TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA
    CCCACCTGTC CAGCGGCTGA TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACTTATGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAGAGC
    TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCGCATC AGACATCCGC ATCTGTGGCC
    CCCTTAGCAC TTGACCTGAA CAAGGCACCA TTGCTGCCCC CCAGCGCCTT GCCCCTGGCT
    CCCAGCAGTA CCCGCCACAG CCAGCCCACT GCTCGAGCCT CATCACAACC TTGGACCATG
    GCCAGCATCC CCAGGACCAC CGCAAGGCCA GTGCTGACCA TGCCTCCCAT GGCCCCCTCC
    CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGCGGT
    GACGAGGAGC TGAGCGGTGA CTTGGAGGCC AGTGGGACTG GCTCAGGAGG ACTTGAGTCC
    CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC TTGCTACAAC
    TCACCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG CTGGAGCTCG AAGGGGTCGA GGGGCAAGAA
    CTGTTCTACA CGCCGGAGAT GGCGGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG
    AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAATTCAG ACGTTAAGAA GGACTTCCGA
    AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC
    TTTGACCCCA CCACAGCCTT CAGAGCGTCC GACGTGGGCC AGGCCCTGCT CCGGCAGCTC
    CAGGTGTCCA GGCGCAGATC CCTGGGCGTG AAGCGGCCTC TGCAGGAACA CGTGCGGTTC
    ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACAGCC
    AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC
    AGTCACACAA GCCGGCCTGT CAGCAGGACC ACACCTCCCC TGACCACGCG CCAGCCCCCC
    ACCACTGCCC CTAGTCGAGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC
    CGAAGGCCCT GTGACTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCA
    GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACAGAGG GTGCCGTCTG TGAGAAGGCC
    CTGCACCCCT CCGTGCCAGC CTTCGGGGGC TCCTCCTTCC TGGCCTTTCC CACCCTTCGT
    GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG
    CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGCGCTGCT GGGAGGCCGC
    GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACGAGCTC CGTGCCCGTG
    CAGCCCGGCC AGTGGCATCG CCTGGAGCTG TCTCGGCACT GGCGCCAAGG TACTCTGTCC
    GTGGATGGTG AGACTCCTGT CCTGGGCCAG AGCCCCAGTG GCACTGATGG TCTCAACCTG
    GACACTGACC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG
    ACCTCTGTCA GCGTCGGTCT TAGGGGTTGT ATCCGCTTGC TGGACGTCAA CAATCAGCGG
    CTAGAGCTGA GCAGCTGGCC GGAATCTGCG ACTCGAAACT CCGGAGTGGG CGAGTGTGGG
    GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA
    GGCAGGTTCC GCTGTCAGTG CCCACCCGGC CGCTTTGGCC CAACTTGTGC CGACGAGGAC
    CCTTGTCAGC CGAACCCCTG CCATGGAGCA GCCCCCTGCC GCGTGCTGCC TCAGGGAGAG
    GCCAAGTGCG AGTGCCCCCA TGGCCGGGAG GGCAGCCTCT GCCAGACAGT CTCAGAGCCA
    GAGGACAACC AGCCGTTCCT GGCTGATTTC AGCAGCTTCT CCTACCTGGA ACTGAAAGGC
    CTGCACACCT TTGAGCGGGA CCTGGGGGAG AAGATGGCGC TGGAGGTGGT ATTCCTGGCT
    CGGAGCCCCA GCGGCCTGCT CCTCTATAAT GGACAGAAGA CAGATGGCAA GGGTGACTTT
    GTGTCTCTGG CGCTGCACAA TGGCCTCCTG GAGTTCCGCT ACGACCTGGG CAAGGGGGCA
    GCGGTCATCA GGAGCAAGGA GCCGGTGGCC CTGGGTGCTT GGACCAGGGT CTCCCTGGAG
    CGAAATGGAC GCAAAGGAGC CATGCGGGTT GGCGATGGGC CTCGCGTGCT GGGGGAGTCC
    CCGAAATCCC GCAAGGTGCC ACACACCGTT CTCAACCTAA AGGAGCCACT CTTTGTTGGG
    GGGGCCCCTG ATTTCAGCAA GCTGGCCCGA GCAGCTGCCG TGTCCTCTGG CTTTGATGGA
    GCCATCCAGC TGGTCTCCCT GAATGGCCGC CAACTGCTGA CCCGTGAGCA TGTGGTCCGG
    GCCATGGACG TCTCGTCCTT TGCAGACCAC CCTTGTACCC AGGCCGAGGG TCAGCCTTGC
    CTCCATGGGG CTTCCTGTCT CCCCCGTGAG GCCTCCTATG AGTGCCTGTG TCCTGCAGGC
    TTCTCGGGGC TGCACTGTGA GAAGGGCTTG ATTGAGAAAT CAGCTGGGGA CCTGGACGCA
    CTGGCTTTTG ATGGACGGAC CTACATCGAG TACCTCAACG CTGTGACCGA GAGCGAACTG
    ACCAATGAGA TCCCAGCTGA GAAGGCACTC CAGAGCAACC ACTTTGAGCT GAGCCTGCGC
    ACTGAGGCCA CACAGGGGCT GGTGCTGTGG AGTGGCAAGG CCACGGAGCG GGCTGACTAC
    ATCGCACTGG CCATCGTGGA CGGGCGCCTG CAGCTGGCTT ACGACCTGGG CTCCCAGCCC
    GTGGTGCTGC GCTCCACCGT ACCAGTCAAT ACCAACCGCT GGTTGCGGAT CAGGGCACAC
    AGGAAACAGA GGGAAGGTTC CCTTCAGGTG GGCAACGAGG CCCCTGTGAC TGGCTCCTCC
    CCACTGGGTG CCACGCAGCT GGACACAGAT GGAGCCCTGT GGCTGGGTGG CCTGGGGAAG
    CTGCCCACGG GCCAGGCCCT GCCCAAAGCC TATGGCACAG GCTTTGTGGG CTGCCTGCGG
    GACGTGGTGG TGGGCCAGCG CCCGCTGCAC CTGCTGGAGG ACGCCATCAC CAAGCCAGAG
    CTTCGGCCCT GCCCTGCCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv18905
    Clone ID Related Accession (Same CDS sequence) XM_012138580.2
    Accession Version XM_012138580.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC GCAGGGTCTC CGTCCTGGTG 
    CGGTATTTCA TGATCCCCTG TAATGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC
    TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC
    CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TTGGCGCTGT CTGCGAGCCC
    AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCGCCTG CCCCAGCGTG
    GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGAG GCGCATCCGC CTGCTGCGCC GCGGGCCCTG CGGGACCCGG
    GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCCTC TGCGGATGGA
    CTGACAGCTA CGTGCTTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAACG CCCTGTGTGC
    GGCAGCGACG GCAGTGACTA CCCCAGTGAG TGCCAGCTCT TGCGCCAAGC CTGTGCCCAC
    CAAGAGAACG TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTCCCTCAGT
    GACCTGAGCC GTACCTGCCG TGTGAACCCG CGCACGCGGC AGCCGGAGAT GCTCCTGCGA
    CCAGAGAGCT GCCCTCCCCG TGGGACACCC GTGTGTGGGG ACGACGGGGT TACTTACGAT
    AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCGAGGCC TCCTCCTGCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC
    CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGTGACCGCG TCGTCTGTGA CGGGGCCTAC
    AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC
    GAGTGTCAGC AGCAACGTAG CATCCCCATG AAGCACCAGG GCCCGTGTGA CCAGCCCCCA
    TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCGACGT GTACTGTGAA GAATGGGGAA
    GCCGAGTGTG CATGTCACCA GGTGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT
    GGCGTCACGT ATGGCAGTGT GTGCGAACTG GAAGCTTCAG CCTGTGCCCT TAGGCGGGAG
    ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG
    TGTGAAGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT
    GTGTGTGGCT CTGACGGGCG CACATATGCC AATGAATGTG AACTACACGT CCACGCCTGC
    ACACGTCAGA TCAGTCTGCA CGTGGCCTCA GCCAGTCCCT GCCAGACCTG TGGAGATACA
    GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC
    CCTCCACCCG GCCCCGTGTG TGGCAGTGAT GGCGTGACCT ACGGCAGTTC ATGTGAGCTC
    CGTGAGGCTG CCTGTCAGCA GCAGACACAG ATCGAGGAAG CCCGGGTGGG GCCATGTGAG
    CAAGCTGAGT GTGGCTCCGG AGGCTCAGGT TCGGGAGAGG ACGGCGAGTG TGAGCCTGAA
    CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGATGGGCC ATGCGTCTGC
    GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC
    CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG
    GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCATTGC CACCTGCGAT TCCCCTGCAC
    TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTATGG CGGCACCTGT
    GACCCAGTCA CAGGCCAGTG CTCCTGCCGC CCGGGTGTGG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ATGGCCGGAG CGGCTGTACC
    CCCTGCAGCT GTGACCCCCG GGGCGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA
    TGCTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCT
    CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCAACCCA CGACTTGTGC TGAGATGCTT
    TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA
    CCCACCTGTC CAGCGGCTGA TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACTTATGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAGAGC
    TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCGCATC AGACATCCGC ATCTGTGGCC
    CCCTTAGCAC TTGACCTGAA CAAGGCACCA TTGCTGCCCC CCAGCGCCTT GCCCCTGGCT
    CCCAGCAGTA CCCGCCACAG CCAGCCCACT GCTCGAGCCT CATCACAACC TTGGACCATG
    GCCAGCATCC CCAGGACCAC CGCAAGGCCA GTGCTGACCA TGCCTCCCAT GGCCCCCTCC
    CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGCGGT
    GACGAGGAGC TGAGCGGTGA CTTGGAGGCC AGTGGGACTG GCTCAGGAGG ACTTGAGTCC
    CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC TTGCTACAAC
    TCACCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG CTGGAGCTCG AAGGGGTCGA GGGGCAAGAA
    CTGTTCTACA CGCCGGAGAT GGCGGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG
    AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAATTCAG ACGTTAAGAA GGACTTCCGA
    AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC
    TTTGACCCCA CCACAGCCTT CAGAGCGTCC GACGTGGGCC AGGCCCTGCT CCGGCAGCTC
    CAGGTGTCCA GGCGCAGATC CCTGGGCGTG AAGCGGCCTC TGCAGGAACA CGTGCGGTTC
    ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACAGCC
    AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC
    AGTCACACAA GCCGGCCTGT CAGCAGGACC ACACCTCCCC TGACCACGCG CCAGCCCCCC
    ACCACTGCCC CTAGTCGAGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC
    CGAAGGCCCT GTGACTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCA
    GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACAGAGG GTGCCGTCTG TGAGAAGGCC
    CTGCACCCCT CCGTGCCAGC CTTCGGGGGC TCCTCCTTCC TGGCCTTTCC CACCCTTCGT
    GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG
    CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGCGCTGCT GGGAGGCCGC
    GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACGAGCTC CGTGCCCGTG
    CAGCCCGGCC AGTGGCATCG CCTGGAGCTG TCTCGGCACT GGCGCCAAGG TACTCTGTCC
    GTGGATGGTG AGACTCCTGT CCTGGGCCAG AGCCCCAGTG GCACTGATGG TCTCAACCTG
    GACACTGACC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG
    ACCTCTGTCA GCGTCGGTCT TAGGGGTTGT ATCCGCTTGC TGGACGTCAA CAATCAGCGG
    CTAGAGCTGA GCAGCTGGCC GGAATCTGCG ACTCGAAACT CCGGAGTGGG CGAGTGTGGG
    GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA
    GGCAGGTTCC GCTGTCAGTG CCCACCCGGC CGCTTTGGCC CAACTTGTGC CGACGAGGAC
    CCTTGTCAGC CGAACCCCTG CCATGGAGCA GCCCCCTGCC GCGTGCTGCC TCAGGGAGAG
    GCCAAGTGCG AGTGCCCCCA TGGCCGGGAG GGCAGCCTCT GCCAGACAGT CTCAGAGCCA
    GAGGACAACC AGCCGTTCCT GGCTGATTTC AGCAGCTTCT CCTACCTGGA ACTGAAAGGC
    CTGCACACCT TTGAGCGGGA CCTGGGGGAG AAGATGGCGC TGGAGGTGGT ATTCCTGGCT
    CGGAGCCCCA GCGGCCTGCT CCTCTATAAT GGACAGAAGA CAGATGGCAA GGGTGACTTT
    GTGTCTCTGG CGCTGCACAA TGGCCTCCTG GAGTTCCGCT ACGACCTGGG CAAGGGGGCA
    GCGGTCATCA GGAGCAAGGA GCCGGTGGCC CTGGGTGCTT GGACCAGGGT CTCCCTGGAG
    CGAAATGGAC GCAAAGGAGC CATGCGGGTT GGCGATGGGC CTCGCGTGCT GGGGGAGTCC
    CCGAAATCCC GCAAGGTGCC ACACACCGTT CTCAACCTAA AGGAGCCACT CTTTGTTGGG
    GGGGCCCCTG ATTTCAGCAA GCTGGCCCGA GCAGCTGCCG TGTCCTCTGG CTTTGATGGA
    GCCATCCAGC TGGTCTCCCT GAATGGCCGC CAACTGCTGA CCCGTGAGCA TGTGGTCCGG
    GCCATGGACG TCTCGTCCTT TGCAGACCAC CCTTGTACCC AGGCCGAGGG TCAGCCTTGC
    CTCCATGGGG CTTCCTGTCT CCCCCGTGAG GCCTCCTATG AGTGCCTGTG TCCTGCAGGC
    TTCTCGGGGC TGCACTGTGA GAAGGGCTTG ATTGAGAAAT CAGCTGGGGA CCTGGACGCA
    CTGGCTTTTG ATGGACGGAC CTACATCGAG TACCTCAACG CTGTGACCGA GAGTGAGAAG
    GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG
    CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG
    CGCCTGCAGC TGGCTTACGA CCTGGGCTCC CAGCCCGTGG TGCTGCGCTC CACCGTACCA
    GTCAATACCA ACCGCTGGTT GCGGATCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT
    CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC
    ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GGGAAGCTGC CCACGGGCCA GGCCCTGCCC
    AAAGCCTATG GCACAGGCTT TGTGGGCTGC CTGCGGGACG TGGTGGTGGG CCAGCGCCCG
    CTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993970.1
    CDS1..5820
    Translation

    Target ORF information:

    RefSeq Version XM_012138580.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138580.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC GCAGGGTCTC CGTCCTGGTG 
    CGGTATTTCA TGATCCCCTG TAATGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC
    TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC
    CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TTGGCGCTGT CTGCGAGCCC
    AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCGCCTG CCCCAGCGTG
    GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGAG GCGCATCCGC CTGCTGCGCC GCGGGCCCTG CGGGACCCGG
    GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCCTC TGCGGATGGA
    CTGACAGCTA CGTGCTTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAACG CCCTGTGTGC
    GGCAGCGACG GCAGTGACTA CCCCAGTGAG TGCCAGCTCT TGCGCCAAGC CTGTGCCCAC
    CAAGAGAACG TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTCCCTCAGT
    GACCTGAGCC GTACCTGCCG TGTGAACCCG CGCACGCGGC AGCCGGAGAT GCTCCTGCGA
    CCAGAGAGCT GCCCTCCCCG TGGGACACCC GTGTGTGGGG ACGACGGGGT TACTTACGAT
    AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCGAGGCC TCCTCCTGCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC
    CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGTGACCGCG TCGTCTGTGA CGGGGCCTAC
    AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC
    GAGTGTCAGC AGCAACGTAG CATCCCCATG AAGCACCAGG GCCCGTGTGA CCAGCCCCCA
    TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCGACGT GTACTGTGAA GAATGGGGAA
    GCCGAGTGTG CATGTCACCA GGTGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT
    GGCGTCACGT ATGGCAGTGT GTGCGAACTG GAAGCTTCAG CCTGTGCCCT TAGGCGGGAG
    ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG
    TGTGAAGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT
    GTGTGTGGCT CTGACGGGCG CACATATGCC AATGAATGTG AACTACACGT CCACGCCTGC
    ACACGTCAGA TCAGTCTGCA CGTGGCCTCA GCCAGTCCCT GCCAGACCTG TGGAGATACA
    GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC
    CCTCCACCCG GCCCCGTGTG TGGCAGTGAT GGCGTGACCT ACGGCAGTTC ATGTGAGCTC
    CGTGAGGCTG CCTGTCAGCA GCAGACACAG ATCGAGGAAG CCCGGGTGGG GCCATGTGAG
    CAAGCTGAGT GTGGCTCCGG AGGCTCAGGT TCGGGAGAGG ACGGCGAGTG TGAGCCTGAA
    CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGATGGGCC ATGCGTCTGC
    GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC
    CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG
    GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCATTGC CACCTGCGAT TCCCCTGCAC
    TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTATGG CGGCACCTGT
    GACCCAGTCA CAGGCCAGTG CTCCTGCCGC CCGGGTGTGG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ATGGCCGGAG CGGCTGTACC
    CCCTGCAGCT GTGACCCCCG GGGCGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA
    TGCTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCT
    CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCAACCCA CGACTTGTGC TGAGATGCTT
    TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA
    CCCACCTGTC CAGCGGCTGA TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACTTATGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAGAGC
    TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCGCATC AGACATCCGC ATCTGTGGCC
    CCCTTAGCAC TTGACCTGAA CAAGGCACCA TTGCTGCCCC CCAGCGCCTT GCCCCTGGCT
    CCCAGCAGTA CCCGCCACAG CCAGCCCACT GCTCGAGCCT CATCACAACC TTGGACCATG
    GCCAGCATCC CCAGGACCAC CGCAAGGCCA GTGCTGACCA TGCCTCCCAT GGCCCCCTCC
    CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGCGGT
    GACGAGGAGC TGAGCGGTGA CTTGGAGGCC AGTGGGACTG GCTCAGGAGG ACTTGAGTCC
    CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC TTGCTACAAC
    TCACCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG CTGGAGCTCG AAGGGGTCGA GGGGCAAGAA
    CTGTTCTACA CGCCGGAGAT GGCGGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG
    AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAATTCAG ACGTTAAGAA GGACTTCCGA
    AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC
    TTTGACCCCA CCACAGCCTT CAGAGCGTCC GACGTGGGCC AGGCCCTGCT CCGGCAGCTC
    CAGGTGTCCA GGCGCAGATC CCTGGGCGTG AAGCGGCCTC TGCAGGAACA CGTGCGGTTC
    ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACAGCC
    AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC
    AGTCACACAA GCCGGCCTGT CAGCAGGACC ACACCTCCCC TGACCACGCG CCAGCCCCCC
    ACCACTGCCC CTAGTCGAGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC
    CGAAGGCCCT GTGACTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCA
    GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACAGAGG GTGCCGTCTG TGAGAAGGCC
    CTGCACCCCT CCGTGCCAGC CTTCGGGGGC TCCTCCTTCC TGGCCTTTCC CACCCTTCGT
    GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG
    CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGCGCTGCT GGGAGGCCGC
    GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACGAGCTC CGTGCCCGTG
    CAGCCCGGCC AGTGGCATCG CCTGGAGCTG TCTCGGCACT GGCGCCAAGG TACTCTGTCC
    GTGGATGGTG AGACTCCTGT CCTGGGCCAG AGCCCCAGTG GCACTGATGG TCTCAACCTG
    GACACTGACC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG
    ACCTCTGTCA GCGTCGGTCT TAGGGGTTGT ATCCGCTTGC TGGACGTCAA CAATCAGCGG
    CTAGAGCTGA GCAGCTGGCC GGAATCTGCG ACTCGAAACT CCGGAGTGGG CGAGTGTGGG
    GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA
    GGCAGGTTCC GCTGTCAGTG CCCACCCGGC CGCTTTGGCC CAACTTGTGC CGACGAGGAC
    CCTTGTCAGC CGAACCCCTG CCATGGAGCA GCCCCCTGCC GCGTGCTGCC TCAGGGAGAG
    GCCAAGTGCG AGTGCCCCCA TGGCCGGGAG GGCAGCCTCT GCCAGACAGT CTCAGAGCCA
    GAGGACAACC AGCCGTTCCT GGCTGATTTC AGCAGCTTCT CCTACCTGGA ACTGAAAGGC
    CTGCACACCT TTGAGCGGGA CCTGGGGGAG AAGATGGCGC TGGAGGTGGT ATTCCTGGCT
    CGGAGCCCCA GCGGCCTGCT CCTCTATAAT GGACAGAAGA CAGATGGCAA GGGTGACTTT
    GTGTCTCTGG CGCTGCACAA TGGCCTCCTG GAGTTCCGCT ACGACCTGGG CAAGGGGGCA
    GCGGTCATCA GGAGCAAGGA GCCGGTGGCC CTGGGTGCTT GGACCAGGGT CTCCCTGGAG
    CGAAATGGAC GCAAAGGAGC CATGCGGGTT GGCGATGGGC CTCGCGTGCT GGGGGAGTCC
    CCGAAATCCC GCAAGGTGCC ACACACCGTT CTCAACCTAA AGGAGCCACT CTTTGTTGGG
    GGGGCCCCTG ATTTCAGCAA GCTGGCCCGA GCAGCTGCCG TGTCCTCTGG CTTTGATGGA
    GCCATCCAGC TGGTCTCCCT GAATGGCCGC CAACTGCTGA CCCGTGAGCA TGTGGTCCGG
    GCCATGGACG TCTCGTCCTT TGCAGACCAC CCTTGTACCC AGGCCGAGGG TCAGCCTTGC
    CTCCATGGGG CTTCCTGTCT CCCCCGTGAG GCCTCCTATG AGTGCCTGTG TCCTGCAGGC
    TTCTCGGGGC TGCACTGTGA GAAGGGCTTG ATTGAGAAAT CAGCTGGGGA CCTGGACGCA
    CTGGCTTTTG ATGGACGGAC CTACATCGAG TACCTCAACG CTGTGACCGA GAGTGAGAAG
    GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG
    CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG
    CGCCTGCAGC TGGCTTACGA CCTGGGCTCC CAGCCCGTGG TGCTGCGCTC CACCGTACCA
    GTCAATACCA ACCGCTGGTT GCGGATCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT
    CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC
    ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GGGAAGCTGC CCACGGGCCA GGCCCTGCCC
    AAAGCCTATG GCACAGGCTT TGTGGGCTGC CTGCGGGACG TGGTGGTGGG CCAGCGCCCG
    CTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv18906
    Clone ID Related Accession (Same CDS sequence) XM_012138581.2
    Accession Version XM_012138581.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5394bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC GCAGGGTCTC CGTCCTGGTG 
    CGGTATTTCA TGATCCCCTG TAATGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC
    TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC
    CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TTGGCGCTGT CTGCGAGCCC
    AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCGCCTG CCCCAGCGTG
    GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGAG GCGCATCCGC CTGCTGCGCC GCGGGCCCTG CGGGACCCGG
    GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCCTC TGCGGATGGA
    CTGACAGCTA CGTGCTTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAACG CCCTGTGTGC
    GGCAGCGACG GCAGTGACTA CCCCAGTGAG TGCCAGCTCT TGCGCCAAGC CTGTGCCCAC
    CAAGAGAACG TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTCCCTCAGT
    GACCTGAGCC GTACCTGCCG TGTGAACCCG CGCACGCGGC AGCCGGAGAT GCTCCTGCGA
    CCAGAGAGCT GCCCTCCCCG TGGGACACCC GTGTGTGGGG ACGACGGGGT TACTTACGAT
    AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCGAGGCC TCCTCCTGCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC
    CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGTGACCGCG TCGTCTGTGA CGGGGCCTAC
    AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC
    GAGTGTCAGC AGCAACGTAG CATCCCCATG AAGCACCAGG GCCCGTGTGA CCAGCCCCCA
    TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCGACGT GTACTGTGAA GAATGGGGAA
    GCCGAGTGTG CATGTCACCA GGTGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT
    GGCGTCACGT ATGGCAGTGT GTGCGAACTG GAAGCTTCAG CCTGTGCCCT TAGGCGGGAG
    ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG
    TGTGAAGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT
    GTGTGTGGCT CTGACGGGCG CACATATGCC AATGAATGTG AACTACACGT CCACGCCTGC
    ACACGTCAGA TCAGTCTGCA CGTGGCCTCA GCCAGTCCCT GCCAGACCTG TGGAGATACA
    GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC
    CCTCCACCCG GCCCCGTGTG TGGCAGTGAT GGCGTGACCT ACGGCAGTTC ATGTGAGCTC
    CGTGAGGCTG CCTGTCAGCA GCAGACACAG ATCGAGGAAG CCCGGGTGGG GCCATGTGAG
    CAAGCTGAGT GTGGCTCCGG AGGCTCAGGT TCGGGAGAGG ACGGCGAGTG TGAGCCTGAA
    CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGATGGGCC ATGCGTCTGC
    GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC
    CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG
    GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCATTGC CACCTGCGAT TCCCCTGCAC
    TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTATGG CGGCACCTGT
    GACCCAGTCA CAGGCCAGTG CTCCTGCCGC CCGGGTGTGG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ATGGCCGGAG CGGCTGTACC
    CCCTGCAGCT GTGACCCCCG GGGCGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA
    TGCTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCT
    CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCAACCCA CGACTTGTGC TGAGATGCTT
    TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA
    CCCACCTGTC CAGCGGCTGA TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACTTATGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAGAGC
    TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCGCATC AGACATCCGC ATCTGTGGCC
    CCCTTAGCAC TTGACCTGAA CAAGGCACCA TTGCTGCCCC CCAGCGCCTT GCCCCTGGCT
    CCCAGCAGTA CCCGCCACAG CCAGCCCACT GCTCGAGCCT CATCACAACC TTGGACCATG
    GCCAGCATCC CCAGGACCAC CGCAAGGCCA GTGCTGACCA TGCCTCCCAT GGCCCCCTCC
    CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGCGGT
    GACGAGGAGC TGAGCGGTGA CTTGGAGGCC AGTGGGACTG GCTCAGGAGG ACTTGAGTCC
    CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC TTGCTACAAC
    TCACCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG CTGGAGCTCG AAGGGGTCGA GGGGCAAGAA
    CTGTTCTACA CGCCGGAGAT GGCGGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG
    AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAATTCAG ACGTTAAGAA GGACTTCCGA
    AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC
    TTTGACCCCA CCACAGCCTT CAGAGCGTCC GACGTGGGCC AGGCCCTGCT CCGGCAGCTC
    CAGGTGTCCA GGCGCAGATC CCTGGGCGTG AAGCGGCCTC TGCAGGAACA CGTGCGGTTC
    ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACAGCC
    AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC
    AGTCACACAA GCCGGCCTGT CAGCAGGACC ACACCTCCCC TGACCACGCG CCAGCCCCCC
    ACCACTGCCC CTAGTCGAGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC
    CGAAGGCCCT GTGACTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCA
    GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACAGAGG GTGCCGTCTG TGAGAAGGCC
    CTGCACCCCT CCGTGCCAGC CTTCGGGGGC TCCTCCTTCC TGGCCTTTCC CACCCTTCGT
    GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG
    CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGCGCTGCT GGGAGGCCGC
    GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACGAGCTC CGTGCCCGTG
    CAGCCCGGCC AGTGGCATCG CCTGGAGCTG TCTCGGCACT GGCGCCAAGG TACTCTGTCC
    GTGGATGGTG AGACTCCTGT CCTGGGCCAG AGCCCCAGTG GCACTGATGG TCTCAACCTG
    GACACTGACC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG
    ACCTCTGTCA GCGTCGGTCT TAGGGGTTGT ATCCGCTTGC TGGACGTCAA CAATCAGCGG
    CTAGAGCTGA GCAGCTGGCC GGAATCTGCG ACTCGAAACT CCGGAGTGGG CGAGTGTGGG
    GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA
    GGCAGGTTCC GCTGTCAGTG CCCACCCGGC CGCTTTGGCC CAACTTGTGC CGACGAGGAC
    CCTTGTCAGC CGAACCCCTG CCATGGAGCA GCCCCCTGCC GCGTGCTGCC TCAGGGAGAG
    GCCAAGTGCG AGTGCCCCCA TGGCCGGGAG GGCAGCCTCT GCCAGACAGT CTCAGAGCCA
    GAGGACAACC AGCCGTTCCT GGCTGATTTC AGCAGCTTCT CCTACCTGGA ACTGAAAGGC
    CTGCACACCT TTGAGCGGGA CCTGGGGGAG AAGATGGCGC TGGAGGTGGT ATTCCTGGCT
    CGGAGCCCCA GCGGCCTGCT CCTCTATAAT GGACAGAAGA CAGATGGCAA GGGTGACTTT
    GTGTCTCTGG CGCTGCACAA TGGCCTCCTG GAGTTCCGCT ACGACCTGGG CAAGGGGGCA
    GCGGTCATCA GGAGCAAGGA GCCGGTGGCC CTGGGTGCTT GGACCAGGGT CTCCCTGGAG
    CGAAATGGAC GCAAAGGAGC CATGCGGGTT GGCGATGGGC CTCGCGTGCT GGGGGAGTCC
    CCGAAATCCC GCAAGGTGCC ACACACCGTT CTCAACCTAA AGGAGCCACT CTTTGTTGGG
    GGGGCCCCTG ATTTCAGCAA GCTGGCCCGA GCAGCTGCCG TGTCCTCTGG CTTTGATGGA
    GCCATCCAGC TGGTCTCCCT GAATGGCCGC CAACTGCTGA CCCGTGAGCA TGTGGTCCGG
    GCCATGGACG TCTCGTCCTT TGCAGACCAC CCTTGTACCC AGGCCGAGGG TCAGCCTTGC
    CTCCATGGGG CTTCCTGTCT CCCCCGTGAG GCCTCCTATG AGTGCCTGTG TCCTGCAGGC
    TTCTCGGGGC TGCACTGTGA GAAGGGCTTG ATTGAGAAAT CAGCTGGGGA CCTGGACGCA
    CTGGCTTTTG ATGGACGGAC CTACATCGAG TACCTCAACG CTGTGACCGA GAGCGAACTG
    ACCAATGAGA TCCCAGCCCC TGAAACTCTG GATTCCGGGG CCCGTCCCAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993971.1
    CDS1..5394
    Translation

    Target ORF information:

    RefSeq Version XM_012138581.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138581.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC GCAGGGTCTC CGTCCTGGTG 
    CGGTATTTCA TGATCCCCTG TAATGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC
    TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC
    CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TTGGCGCTGT CTGCGAGCCC
    AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCGCCTG CCCCAGCGTG
    GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGAG GCGCATCCGC CTGCTGCGCC GCGGGCCCTG CGGGACCCGG
    GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCCTC TGCGGATGGA
    CTGACAGCTA CGTGCTTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAACG CCCTGTGTGC
    GGCAGCGACG GCAGTGACTA CCCCAGTGAG TGCCAGCTCT TGCGCCAAGC CTGTGCCCAC
    CAAGAGAACG TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTCCCTCAGT
    GACCTGAGCC GTACCTGCCG TGTGAACCCG CGCACGCGGC AGCCGGAGAT GCTCCTGCGA
    CCAGAGAGCT GCCCTCCCCG TGGGACACCC GTGTGTGGGG ACGACGGGGT TACTTACGAT
    AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCGAGGCC TCCTCCTGCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC
    CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGTGACCGCG TCGTCTGTGA CGGGGCCTAC
    AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC
    GAGTGTCAGC AGCAACGTAG CATCCCCATG AAGCACCAGG GCCCGTGTGA CCAGCCCCCA
    TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCGACGT GTACTGTGAA GAATGGGGAA
    GCCGAGTGTG CATGTCACCA GGTGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT
    GGCGTCACGT ATGGCAGTGT GTGCGAACTG GAAGCTTCAG CCTGTGCCCT TAGGCGGGAG
    ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG
    TGTGAAGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT
    GTGTGTGGCT CTGACGGGCG CACATATGCC AATGAATGTG AACTACACGT CCACGCCTGC
    ACACGTCAGA TCAGTCTGCA CGTGGCCTCA GCCAGTCCCT GCCAGACCTG TGGAGATACA
    GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC
    CCTCCACCCG GCCCCGTGTG TGGCAGTGAT GGCGTGACCT ACGGCAGTTC ATGTGAGCTC
    CGTGAGGCTG CCTGTCAGCA GCAGACACAG ATCGAGGAAG CCCGGGTGGG GCCATGTGAG
    CAAGCTGAGT GTGGCTCCGG AGGCTCAGGT TCGGGAGAGG ACGGCGAGTG TGAGCCTGAA
    CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGATGGGCC ATGCGTCTGC
    GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC
    CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG
    GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCATTGC CACCTGCGAT TCCCCTGCAC
    TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTATGG CGGCACCTGT
    GACCCAGTCA CAGGCCAGTG CTCCTGCCGC CCGGGTGTGG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ATGGCCGGAG CGGCTGTACC
    CCCTGCAGCT GTGACCCCCG GGGCGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA
    TGCTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCT
    CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCAACCCA CGACTTGTGC TGAGATGCTT
    TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA
    CCCACCTGTC CAGCGGCTGA TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACTTATGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAGAGC
    TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCGCATC AGACATCCGC ATCTGTGGCC
    CCCTTAGCAC TTGACCTGAA CAAGGCACCA TTGCTGCCCC CCAGCGCCTT GCCCCTGGCT
    CCCAGCAGTA CCCGCCACAG CCAGCCCACT GCTCGAGCCT CATCACAACC TTGGACCATG
    GCCAGCATCC CCAGGACCAC CGCAAGGCCA GTGCTGACCA TGCCTCCCAT GGCCCCCTCC
    CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGCGGT
    GACGAGGAGC TGAGCGGTGA CTTGGAGGCC AGTGGGACTG GCTCAGGAGG ACTTGAGTCC
    CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC TTGCTACAAC
    TCACCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG CTGGAGCTCG AAGGGGTCGA GGGGCAAGAA
    CTGTTCTACA CGCCGGAGAT GGCGGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG
    AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAATTCAG ACGTTAAGAA GGACTTCCGA
    AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC
    TTTGACCCCA CCACAGCCTT CAGAGCGTCC GACGTGGGCC AGGCCCTGCT CCGGCAGCTC
    CAGGTGTCCA GGCGCAGATC CCTGGGCGTG AAGCGGCCTC TGCAGGAACA CGTGCGGTTC
    ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACAGCC
    AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC
    AGTCACACAA GCCGGCCTGT CAGCAGGACC ACACCTCCCC TGACCACGCG CCAGCCCCCC
    ACCACTGCCC CTAGTCGAGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC
    CGAAGGCCCT GTGACTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCA
    GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACAGAGG GTGCCGTCTG TGAGAAGGCC
    CTGCACCCCT CCGTGCCAGC CTTCGGGGGC TCCTCCTTCC TGGCCTTTCC CACCCTTCGT
    GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG
    CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGCGCTGCT GGGAGGCCGC
    GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACGAGCTC CGTGCCCGTG
    CAGCCCGGCC AGTGGCATCG CCTGGAGCTG TCTCGGCACT GGCGCCAAGG TACTCTGTCC
    GTGGATGGTG AGACTCCTGT CCTGGGCCAG AGCCCCAGTG GCACTGATGG TCTCAACCTG
    GACACTGACC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG
    ACCTCTGTCA GCGTCGGTCT TAGGGGTTGT ATCCGCTTGC TGGACGTCAA CAATCAGCGG
    CTAGAGCTGA GCAGCTGGCC GGAATCTGCG ACTCGAAACT CCGGAGTGGG CGAGTGTGGG
    GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA
    GGCAGGTTCC GCTGTCAGTG CCCACCCGGC CGCTTTGGCC CAACTTGTGC CGACGAGGAC
    CCTTGTCAGC CGAACCCCTG CCATGGAGCA GCCCCCTGCC GCGTGCTGCC TCAGGGAGAG
    GCCAAGTGCG AGTGCCCCCA TGGCCGGGAG GGCAGCCTCT GCCAGACAGT CTCAGAGCCA
    GAGGACAACC AGCCGTTCCT GGCTGATTTC AGCAGCTTCT CCTACCTGGA ACTGAAAGGC
    CTGCACACCT TTGAGCGGGA CCTGGGGGAG AAGATGGCGC TGGAGGTGGT ATTCCTGGCT
    CGGAGCCCCA GCGGCCTGCT CCTCTATAAT GGACAGAAGA CAGATGGCAA GGGTGACTTT
    GTGTCTCTGG CGCTGCACAA TGGCCTCCTG GAGTTCCGCT ACGACCTGGG CAAGGGGGCA
    GCGGTCATCA GGAGCAAGGA GCCGGTGGCC CTGGGTGCTT GGACCAGGGT CTCCCTGGAG
    CGAAATGGAC GCAAAGGAGC CATGCGGGTT GGCGATGGGC CTCGCGTGCT GGGGGAGTCC
    CCGAAATCCC GCAAGGTGCC ACACACCGTT CTCAACCTAA AGGAGCCACT CTTTGTTGGG
    GGGGCCCCTG ATTTCAGCAA GCTGGCCCGA GCAGCTGCCG TGTCCTCTGG CTTTGATGGA
    GCCATCCAGC TGGTCTCCCT GAATGGCCGC CAACTGCTGA CCCGTGAGCA TGTGGTCCGG
    GCCATGGACG TCTCGTCCTT TGCAGACCAC CCTTGTACCC AGGCCGAGGG TCAGCCTTGC
    CTCCATGGGG CTTCCTGTCT CCCCCGTGAG GCCTCCTATG AGTGCCTGTG TCCTGCAGGC
    TTCTCGGGGC TGCACTGTGA GAAGGGCTTG ATTGAGAAAT CAGCTGGGGA CCTGGACGCA
    CTGGCTTTTG ATGGACGGAC CTACATCGAG TACCTCAACG CTGTGACCGA GAGCGAACTG
    ACCAATGAGA TCCCAGCCCC TGAAACTCTG GATTCCGGGG CCCGTCCCAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61814
    Clone ID Related Accession (Same CDS sequence) XM_027975848.1
    Accession Version XM_027975848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product agrin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GGCACCCAGC AGCCCCGAAG GCCCTGTGAC
    TCCCAGCCCT GCCTCCATGG GGGGACCTGC CAGGACCAGG GCTCAGGTGC AGACTTCACC
    TGCAGCTGCC CAGCAGGCAC AGAGGGTGCC GTCTGTGAGA AGGCCCTGCA CCCCTCCGTG
    CCAGCCTTCG GGGGCTCCTC CTTCCTGGCC TTTCCCACCC TTCGTGCCTA CCACACCCTG
    CGCCTGGCGC TCGAGTTCCG GGCGCTGGAG CCCCAGGGGC TGCTGCTCTA CAACGGCAAT
    GCCCGGGGCA AGGACTTCCT GGGACTGGCG CTGCTGGGAG GCCGCGTGCA GTTCAGGTTC
    GACACAGGCT CAGGGCCCGC GGTGCTGACG AGCTCCGTGC CCGTGCAGCC CGGCCAGTGG
    CATCGCCTGG AGCTGTCTCG GCACTGGCGC CAAGGTACTC TGTCCGTGGA TGGTGAGACT
    CCTGTCCTGG GCCAGAGCCC CAGTGGCACT GATGGTCTCA ACCTGGACAC TGACCTTTTC
    GTGGGTGGCG TCCCTGAGGA TCAGGCTTCC ACGGTGCTAG AGCGGACCTC TGTCAGCGTC
    GGTCTTAGGG GTTGTATCCG CTTGCTGGAC GTCAACAATC AGCGGCTAGA GCTGAGCAGC
    TGGCCGGAAT CTGCGACTCG AAACTCCGGA GTGGGCGAGT GTGGGGACCA CCCCTGTCTG
    CCTAGCCCCT GCCTCGGAGG GGCCCCGTGC CAGGCCCTGG AGGCAGGCAG GTTCCGCTGT
    CAGTGCCCAC CCGGCCGCTT TGGCCCAACT TGTGCCGACG AGGACCCTTG TCAGCCGAAC
    CCCTGCCATG GAGCAGCCCC CTGCCGCGTG CTGCCTCAGG GAGAGGCCAA GTGCGAGTGC
    CCCCATGGCC GGGAGGGCAG CCTCTGCCAG ACAGTCTCAG AGCCAGAGGA CAACCAGCCG
    TTCCTGGCTG ATTTCAGCAG CTTCTCCTAC CTGGAACTGA AAGGCCTGCA CACCTTTGAG
    CGGGACCTGG GGGAGAAGAT GGCGCTGGAG GTGGTATTCC TGGCTCGGAG CCCCAGCGGC
    CTGCTCCTCT ATAATGGACA GAAGACAGAT GGCAAGGGTG ACTTTGTGTC TCTGGCGCTG
    CACAATGGCC TCCTGGAGTT CCGCTACGAC CTGGGCAAGG GGGCAGCGGT CATCAGGAGC
    AAGGAGCCGG TGGCCCTGGG TGCTTGGACC AGGGTCTCCC TGGAGCGAAA TGGACGCAAA
    GGAGCCATGC GGGTTGGCGA TGGGCCTCGC GTGCTGGGGG AGTCCCCGAA ATCCCGCAAG
    GTGCCACACA CCGTTCTCAA CCTAAAGGAG CCACTCTTTG TTGGGGGGGC CCCTGATTTC
    AGCAAGCTGG CCCGAGCAGC TGCCGTGTCC TCTGGCTTTG ATGGAGCCAT CCAGCTGGTC
    TCCCTGAATG GCCGCCAACT GCTGACCCGT GAGCATGTGG TCCGGGCCAT GGACGTCTCG
    TCCTTTGCAG ACCACCCTTG TACCCAGGCC GAGGGTCAGC CTTGCCTCCA TGGGGCTTCC
    TGTCTCCCCC GTGAGGCCTC CTATGAGTGC CTGTGTCCTG CAGGCTTCTC GGGGCTGCAC
    TGTGAGAAGG GCTTGATTGA GAAATCAGCT GGGGACCTGG ACGCACTGGC TTTTGATGGA
    CGGACCTACA TCGAGTACCT CAACGCTGTG ACCGAGAGCG AACTGACCAA TGAGATCCCA
    GCTGAGAAGG CACTCCAGAG CAACCACTTT GAGCTGAGCC TGCGCACTGA GGCCACACAG
    GGGCTGGTGC TGTGGAGTGG CAAGGCCACG GAGCGGGCTG ACTACATCGC ACTGGCCATC
    GTGGACGGGC GCCTGCAGCT GGCTTACGAC CTGGGCTCCC AGCCCGTGGT GCTGCGCTCC
    ACCGTACCAG TCAATACCAA CCGCTGGTTG CGGATCAGGG CACACAGGAA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA CGAGGCCCCT GTGACTGGCT CCTCCCCACT GGGTGCCACG
    CAGCTGGACA CAGATGGAGC CCTGTGGCTG GGTGGCCTGG GGAAGCTGCC CACGGGCCAG
    GCCCTGCCCA AAGCCTATGG CACAGGCTTT GTGGGCTGCC TGCGGGACGT GGTGGTGGGC
    CAGCGCCCGC TGCACCTGCT GGAGGACGCC ATCACCAAGC CAGAGCTTCG GCCCTGCCCT
    GCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831649.1
    CDS44..5992
    Translation

    Target ORF information:

    RefSeq Version XM_027975848.1
    Organism Ovis aries(sheep)
    Definition Ovis aries agrin (AGRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GGCACCCAGC AGCCCCGAAG GCCCTGTGAC
    TCCCAGCCCT GCCTCCATGG GGGGACCTGC CAGGACCAGG GCTCAGGTGC AGACTTCACC
    TGCAGCTGCC CAGCAGGCAC AGAGGGTGCC GTCTGTGAGA AGGCCCTGCA CCCCTCCGTG
    CCAGCCTTCG GGGGCTCCTC CTTCCTGGCC TTTCCCACCC TTCGTGCCTA CCACACCCTG
    CGCCTGGCGC TCGAGTTCCG GGCGCTGGAG CCCCAGGGGC TGCTGCTCTA CAACGGCAAT
    GCCCGGGGCA AGGACTTCCT GGGACTGGCG CTGCTGGGAG GCCGCGTGCA GTTCAGGTTC
    GACACAGGCT CAGGGCCCGC GGTGCTGACG AGCTCCGTGC CCGTGCAGCC CGGCCAGTGG
    CATCGCCTGG AGCTGTCTCG GCACTGGCGC CAAGGTACTC TGTCCGTGGA TGGTGAGACT
    CCTGTCCTGG GCCAGAGCCC CAGTGGCACT GATGGTCTCA ACCTGGACAC TGACCTTTTC
    GTGGGTGGCG TCCCTGAGGA TCAGGCTTCC ACGGTGCTAG AGCGGACCTC TGTCAGCGTC
    GGTCTTAGGG GTTGTATCCG CTTGCTGGAC GTCAACAATC AGCGGCTAGA GCTGAGCAGC
    TGGCCGGAAT CTGCGACTCG AAACTCCGGA GTGGGCGAGT GTGGGGACCA CCCCTGTCTG
    CCTAGCCCCT GCCTCGGAGG GGCCCCGTGC CAGGCCCTGG AGGCAGGCAG GTTCCGCTGT
    CAGTGCCCAC CCGGCCGCTT TGGCCCAACT TGTGCCGACG AGGACCCTTG TCAGCCGAAC
    CCCTGCCATG GAGCAGCCCC CTGCCGCGTG CTGCCTCAGG GAGAGGCCAA GTGCGAGTGC
    CCCCATGGCC GGGAGGGCAG CCTCTGCCAG ACAGTCTCAG AGCCAGAGGA CAACCAGCCG
    TTCCTGGCTG ATTTCAGCAG CTTCTCCTAC CTGGAACTGA AAGGCCTGCA CACCTTTGAG
    CGGGACCTGG GGGAGAAGAT GGCGCTGGAG GTGGTATTCC TGGCTCGGAG CCCCAGCGGC
    CTGCTCCTCT ATAATGGACA GAAGACAGAT GGCAAGGGTG ACTTTGTGTC TCTGGCGCTG
    CACAATGGCC TCCTGGAGTT CCGCTACGAC CTGGGCAAGG GGGCAGCGGT CATCAGGAGC
    AAGGAGCCGG TGGCCCTGGG TGCTTGGACC AGGGTCTCCC TGGAGCGAAA TGGACGCAAA
    GGAGCCATGC GGGTTGGCGA TGGGCCTCGC GTGCTGGGGG AGTCCCCGAA ATCCCGCAAG
    GTGCCACACA CCGTTCTCAA CCTAAAGGAG CCACTCTTTG TTGGGGGGGC CCCTGATTTC
    AGCAAGCTGG CCCGAGCAGC TGCCGTGTCC TCTGGCTTTG ATGGAGCCAT CCAGCTGGTC
    TCCCTGAATG GCCGCCAACT GCTGACCCGT GAGCATGTGG TCCGGGCCAT GGACGTCTCG
    TCCTTTGCAG ACCACCCTTG TACCCAGGCC GAGGGTCAGC CTTGCCTCCA TGGGGCTTCC
    TGTCTCCCCC GTGAGGCCTC CTATGAGTGC CTGTGTCCTG CAGGCTTCTC GGGGCTGCAC
    TGTGAGAAGG GCTTGATTGA GAAATCAGCT GGGGACCTGG ACGCACTGGC TTTTGATGGA
    CGGACCTACA TCGAGTACCT CAACGCTGTG ACCGAGAGCG AACTGACCAA TGAGATCCCA
    GCTGAGAAGG CACTCCAGAG CAACCACTTT GAGCTGAGCC TGCGCACTGA GGCCACACAG
    GGGCTGGTGC TGTGGAGTGG CAAGGCCACG GAGCGGGCTG ACTACATCGC ACTGGCCATC
    GTGGACGGGC GCCTGCAGCT GGCTTACGAC CTGGGCTCCC AGCCCGTGGT GCTGCGCTCC
    ACCGTACCAG TCAATACCAA CCGCTGGTTG CGGATCAGGG CACACAGGAA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA CGAGGCCCCT GTGACTGGCT CCTCCCCACT GGGTGCCACG
    CAGCTGGACA CAGATGGAGC CCTGTGGCTG GGTGGCCTGG GGAAGCTGCC CACGGGCCAG
    GCCCTGCCCA AAGCCTATGG CACAGGCTTT GTGGGCTGCC TGCGGGACGT GGTGGTGGGC
    CAGCGCCCGC TGCACCTGCT GGAGGACGCC ATCACCAAGC CAGAGCTTCG GCCCTGCCCT
    GCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61815
    Clone ID Related Accession (Same CDS sequence) XM_027975849.1
    Accession Version XM_027975849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product agrin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCGCTGTGG GCAGTGCCGC TTTGGAGCCT TGTGTGAAGC TGAGACTGGG
    CGCTGTGTGT GCCCCTCCGA ATGTGTGGCC TCAGCCCAGC CTGTGTGTGG CTCTGACGGG
    CGCACATATG CCAATGAATG TGAACTACAC GTCCACGCCT GCACACGTCA GATCAGTCTG
    CACGTGGCCT CAGCCAGTCC CTGCCAGACC TGTGGAGATA CAGTGTGTGC CTTTGGGGCT
    GTGTGCTCAG CGGGGCAGTG CATGTGTCCC CGGTGTGAGC GCCCTCCACC CGGCCCCGTG
    TGTGGCAGTG ATGGCGTGAC CTACGGCAGT TCATGTGAGC TCCGTGAGGC TGCCTGTCAG
    CAGCAGACAC AGATCGAGGA AGCCCGGGTG GGGCCATGTG AGCAAGCTGA GTGTGGCTCC
    GGAGGCTCAG GTTCGGGAGA GGACGGCGAG TGTGAGCCTG AACTGTGCCG GCAGCGAGGT
    GGCATCTGGG ATGAGGACTC GGAGGATGGG CCATGCGTCT GCGGCTTCAG CTGCCAGGGC
    GTCCTGAGGA GCCCGGTGTG TGGCTCGGAT GGAGTCACCT ACCGCACCGA ATGTGAGCTG
    AAGAAGGCCA GGTGTGAATC ACAGCCAGAG CTGTATGTCG TGGCCCAGGG AGCCTGCCGT
    GGCCCCACTT TGGCCCCATT GCCACCTGCG ATTCCCCTGC ACTGTGCTCA GACCCCCTAC
    GGTTGCTGCC AGGACAACAT CACTGTTGCC CAGGGTGTGG GCCTGGCTGG CTGCCCCAGC
    TCATGCCAGT GTAACCCACA CGGCTCCTAT GGCGGCACCT GTGACCCAGT CACAGGCCAG
    TGCTCCTGCC GCCCGGGTGT GGGGGGCCTC AAGTGTGACC GCTGTGAGCC TGGCTTCTGG
    AACTTCCGTG GTATCGTCAC TGATGGCCGG AGCGGCTGTA CCCCCTGCAG CTGTGACCCC
    CGGGGCGCCG TGCGGGACGA CTGTGAGCAG ATGACTGGGC TATGCTCCTG TAAGCCTGGG
    GTGGCCGGGC CCAAGTGTGG GCAGTGTCCA GATGGCCGTG CTCTGGGCCC TGCTGGCTGT
    GAAACAGACT CTTCGCAACC CACGACTTGT GCTGAGATGC TTTGTGAGTT TGGGGCATCC
    TGTGTGGAGG AGGCTGGCTC TGCCCACTGC GTGTGCCCAA CACCCACCTG TCCAGCGGCT
    GATGCTACCA AGGTCTGTGG GTCAGATGGA GTCACTTATG GCAATGAGTG CCAGCTGAGG
    ACCATCGCCT GCCGCCAGGG CTTGGAGATC TCTATCCAGA GCTTCGGCCC CTGCCAGGAA
    GGCATCACTT CTGGTCCGCA TCAGACATCC GCATCTGTGG CCCCCTTAGC ACTTGACCTG
    AACAAGGCAC CATTGCTGCC CCCCAGCGCC TTGCCCCTGG CTCCCAGCAG TACCCGCTAC
    AGCCAGCCCA CTGCTCGAGC CTCATCACAA CCTTGGACCA TGGCCAGCAT CCCCAGGACC
    ACCGCAAGGC CAGTGCTGAC CATGCCTCCC ATGGCCCCCT CCCCGGCAGC CAGCCTCGTC
    ACCTCTGCCT TCGGCGAATC TGGCAGCGCT GATGGGAGCG GTGACGAGGA GCTGAGCGGT
    GACTTGGAGG CCAGTGGGAC TGGCTCAGGA GGACTTGAGT CCCCGGAAAG AGACAGTGCT
    GGGACCCCTG GGCTACCCAT GGAAAGGGCT TCTTGCTACA ACTCACCTAT GGGCTGCTGC
    TCAGATGGCA AGACACCCTC CCTGGACGCA GAGGGCTCTA ACTGTCCTGC CACCAAGGCG
    TTCCAGGGTG TGCTGGAGCT CGAAGGGGTC GAGGGGCAAG AACTGTTCTA CACGCCGGAG
    ATGGCGGACC CCAAGTCTGA GCTGTTTGGG GAGACTGCCA GGAGCATTGA GAGCGCACTG
    GATGACCTCT TCCGGAATTC AGACGTTAAG AAGGACTTCC GAAGTGTCCG CCTACGGGAC
    TTGGGGCCAG GCAGCTCGGT CCGGGCCATT GTGGAAGTGC ACTTTGACCC CACCACAGCC
    TTCAGAGCGT CCGACGTGGG CCAGGCCCTG CTCCGGCAGC TCCAGGTGTC CAGGCGCAGA
    TCCCTGGGCG TGAAGCGGCC TCTGCAGGAA CACGTGCGGT TCATGGACTT CGACTGGTTT
    CCTGCATTCT TCACGGGAGC CACCCCAGCT GCAGCCACAG CCAGAGCCAC CACGGTGGCC
    CGCCTGCCAC CTTCAGCTGC AACCCCTCGG GCCCACTTTC CCAGTCACAC AAGCCGGCCT
    GTCAGCAGGA CCACACCTCC CCTGACCACG CGCCAGCCCC CCACCACTGC CCCTAGTCGA
    GTGCCTGGAC GCCGTCCCCT GCCCCCAGGC ACCCAGCAGC CCCGAAGGCC CTGTGACTCC
    CAGCCCTGCC TCCATGGGGG GACCTGCCAG GACCAGGGCT CAGGTGCAGA CTTCACCTGC
    AGCTGCCCAG CAGGCACAGA GGGTGCCGTC TGTGAGAAGG CCCTGCACCC CTCCGTGCCA
    GCCTTCGGGG GCTCCTCCTT CCTGGCCTTT CCCACCCTTC GTGCCTACCA CACCCTGCGC
    CTGGCGCTCG AGTTCCGGGC GCTGGAGCCC CAGGGGCTGC TGCTCTACAA CGGCAATGCC
    CGGGGCAAGG ACTTCCTGGG ACTGGCGCTG CTGGGAGGCC GCGTGCAGTT CAGGTTCGAC
    ACAGGCTCAG GGCCCGCGGT GCTGACGAGC TCCGTGCCCG TGCAGCCCGG CCAGTGGCAT
    CGCCTGGAGC TGTCTCGGCA CTGGCGCCAA GGTACTCTGT CCGTGGATGG TGAGACTCCT
    GTCCTGGGCC AGAGCCCCAG TGGCACTGAT GGTCTCAACC TGGACACTGA CCTTTTCGTG
    GGTGGCGTCC CTGAGGATCA GGCTTCCACG GTGCTAGAGC GGACCTCTGT CAGCGTCGGT
    CTTAGGGGTT GTATCCGCTT GCTGGACGTC AACAATCAGC GGCTAGAGCT GAGCAGCTGG
    CCGGAATCTG CGACTCGAAA CTCCGGAGTG GGCGAGTGTG GGGACCACCC CTGTCTGCCT
    AGCCCCTGCC TCGGAGGGGC CCCGTGCCAG GCCCTGGAGG CAGGCAGGTT CCGCTGTCAG
    TGCCCACCCG GCCGCTTTGG CCCAACTTGT GCCGACGAGG ACCCTTGTCA GCCGAACCCC
    TGCCATGGAG CAGCCCCCTG CCGCGTGCTG CCTCAGGGAG AGGCCAAGTG CGAGTGCCCC
    CATGGCCGGG AGGGCAGCCT CTGCCAGACA GTCTCAGAGC CAGAGGACAA CCAGCCGTTC
    CTGGCTGATT TCAGCAGCTT CTCCTACCTG GAACTGAAAG GCCTGCACAC CTTTGAGCGG
    GACCTGGGGG AGAAGATGGC GCTGGAGGTG GTATTCCTGG CTCGGAGCCC CAGCGGCCTG
    CTCCTCTATA ATGGACAGAA GACAGATGGC AAGGGTGACT TTGTGTCTCT GGCGCTGCAC
    AATGGCCTCC TGGAGTTCCG CTACGACCTG GGCAAGGGGG CAGCGGTCAT CAGGAGCAAG
    GAGCCGGTGG CCCTGGGTGC TTGGACCAGG GTCTCCCTGG AGCGAAATGG ACGCAAAGGA
    GCCATGCGGG TTGGCGATGG GCCTCGCGTG CTGGGGGAGT CCCCGAAATC CCGCAAGGTG
    CCACACACCG TTCTCAACCT AAAGGAGCCA CTCTTTGTTG GGGGGGCCCC TGATTTCAGC
    AAGCTGGCCC GAGCAGCTGC CGTGTCCTCT GGCTTTGATG GAGCCATCCA GCTGGTCTCC
    CTGAATGGCC GCCAACTGCT GACCCGTGAG CATGTGGTCC GGGCCATGGA CGTCTCGTCC
    TTTGCAGACC ACCCTTGTAC CCAGGCCGAG GGTCAGCCTT GCCTCCATGG GGCTTCCTGT
    CTCCCCCGTG AGGCCTCCTA TGAGTGCCTG TGTCCTGCAG GCTTCTCGGG GCTGCACTGT
    GAGAAGGGCT TGATTGAGAA ATCAGCTGGG GACCTGGACG CACTGGCTTT TGATGGACGG
    ACCTACATCG AGTACCTCAA CGCTGTGACC GAGAGCGAAC TGACCAATGA GATCCCAGCT
    GAGAAGGCAC TCCAGAGCAA CCACTTTGAG CTGAGCCTGC GCACTGAGGC CACACAGGGG
    CTGGTGCTGT GGAGTGGCAA GGCCACGGAG CGGGCTGACT ACATCGCACT GGCCATCGTG
    GACGGGCGCC TGCAGCTGGC TTACGACCTG GGCTCCCAGC CCGTGGTGCT GCGCTCCACC
    GTACCAGTCA ATACCAACCG CTGGTTGCGG ATCAGGGCAC ACAGGAAACA GAGGGAAGGT
    TCCCTTCAGG TGGGCAACGA GGCCCCTGTG ACTGGCTCCT CCCCACTGGG TGCCACGCAG
    CTGGACACAG ATGGAGCCCT GTGGCTGGGT GGCCTGGGGA AGCTGCCCAC GGGCCAGGCC
    CTGCCCAAAG CCTATGGCAC AGGCTTTGTG GGCTGCCTGC GGGACGTGGT GGTGGGCCAG
    CGCCCGCTGC ACCTGCTGGA GGACGCCATC ACCAAGCCAG AGCTTCGGCC CTGCCCTGCC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831650.1
    CDS45..5990
    Translation

    Target ORF information:

    RefSeq Version XM_027975849.1
    Organism Ovis aries(sheep)
    Definition Ovis aries agrin (AGRN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCGCTGTGG GCAGTGCCGC TTTGGAGCCT TGTGTGAAGC TGAGACTGGG
    CGCTGTGTGT GCCCCTCCGA ATGTGTGGCC TCAGCCCAGC CTGTGTGTGG CTCTGACGGG
    CGCACATATG CCAATGAATG TGAACTACAC GTCCACGCCT GCACACGTCA GATCAGTCTG
    CACGTGGCCT CAGCCAGTCC CTGCCAGACC TGTGGAGATA CAGTGTGTGC CTTTGGGGCT
    GTGTGCTCAG CGGGGCAGTG CATGTGTCCC CGGTGTGAGC GCCCTCCACC CGGCCCCGTG
    TGTGGCAGTG ATGGCGTGAC CTACGGCAGT TCATGTGAGC TCCGTGAGGC TGCCTGTCAG
    CAGCAGACAC AGATCGAGGA AGCCCGGGTG GGGCCATGTG AGCAAGCTGA GTGTGGCTCC
    GGAGGCTCAG GTTCGGGAGA GGACGGCGAG TGTGAGCCTG AACTGTGCCG GCAGCGAGGT
    GGCATCTGGG ATGAGGACTC GGAGGATGGG CCATGCGTCT GCGGCTTCAG CTGCCAGGGC
    GTCCTGAGGA GCCCGGTGTG TGGCTCGGAT GGAGTCACCT ACCGCACCGA ATGTGAGCTG
    AAGAAGGCCA GGTGTGAATC ACAGCCAGAG CTGTATGTCG TGGCCCAGGG AGCCTGCCGT
    GGCCCCACTT TGGCCCCATT GCCACCTGCG ATTCCCCTGC ACTGTGCTCA GACCCCCTAC
    GGTTGCTGCC AGGACAACAT CACTGTTGCC CAGGGTGTGG GCCTGGCTGG CTGCCCCAGC
    TCATGCCAGT GTAACCCACA CGGCTCCTAT GGCGGCACCT GTGACCCAGT CACAGGCCAG
    TGCTCCTGCC GCCCGGGTGT GGGGGGCCTC AAGTGTGACC GCTGTGAGCC TGGCTTCTGG
    AACTTCCGTG GTATCGTCAC TGATGGCCGG AGCGGCTGTA CCCCCTGCAG CTGTGACCCC
    CGGGGCGCCG TGCGGGACGA CTGTGAGCAG ATGACTGGGC TATGCTCCTG TAAGCCTGGG
    GTGGCCGGGC CCAAGTGTGG GCAGTGTCCA GATGGCCGTG CTCTGGGCCC TGCTGGCTGT
    GAAACAGACT CTTCGCAACC CACGACTTGT GCTGAGATGC TTTGTGAGTT TGGGGCATCC
    TGTGTGGAGG AGGCTGGCTC TGCCCACTGC GTGTGCCCAA CACCCACCTG TCCAGCGGCT
    GATGCTACCA AGGTCTGTGG GTCAGATGGA GTCACTTATG GCAATGAGTG CCAGCTGAGG
    ACCATCGCCT GCCGCCAGGG CTTGGAGATC TCTATCCAGA GCTTCGGCCC CTGCCAGGAA
    GGCATCACTT CTGGTCCGCA TCAGACATCC GCATCTGTGG CCCCCTTAGC ACTTGACCTG
    AACAAGGCAC CATTGCTGCC CCCCAGCGCC TTGCCCCTGG CTCCCAGCAG TACCCGCTAC
    AGCCAGCCCA CTGCTCGAGC CTCATCACAA CCTTGGACCA TGGCCAGCAT CCCCAGGACC
    ACCGCAAGGC CAGTGCTGAC CATGCCTCCC ATGGCCCCCT CCCCGGCAGC CAGCCTCGTC
    ACCTCTGCCT TCGGCGAATC TGGCAGCGCT GATGGGAGCG GTGACGAGGA GCTGAGCGGT
    GACTTGGAGG CCAGTGGGAC TGGCTCAGGA GGACTTGAGT CCCCGGAAAG AGACAGTGCT
    GGGACCCCTG GGCTACCCAT GGAAAGGGCT TCTTGCTACA ACTCACCTAT GGGCTGCTGC
    TCAGATGGCA AGACACCCTC CCTGGACGCA GAGGGCTCTA ACTGTCCTGC CACCAAGGCG
    TTCCAGGGTG TGCTGGAGCT CGAAGGGGTC GAGGGGCAAG AACTGTTCTA CACGCCGGAG
    ATGGCGGACC CCAAGTCTGA GCTGTTTGGG GAGACTGCCA GGAGCATTGA GAGCGCACTG
    GATGACCTCT TCCGGAATTC AGACGTTAAG AAGGACTTCC GAAGTGTCCG CCTACGGGAC
    TTGGGGCCAG GCAGCTCGGT CCGGGCCATT GTGGAAGTGC ACTTTGACCC CACCACAGCC
    TTCAGAGCGT CCGACGTGGG CCAGGCCCTG CTCCGGCAGC TCCAGGTGTC CAGGCGCAGA
    TCCCTGGGCG TGAAGCGGCC TCTGCAGGAA CACGTGCGGT TCATGGACTT CGACTGGTTT
    CCTGCATTCT TCACGGGAGC CACCCCAGCT GCAGCCACAG CCAGAGCCAC CACGGTGGCC
    CGCCTGCCAC CTTCAGCTGC AACCCCTCGG GCCCACTTTC CCAGTCACAC AAGCCGGCCT
    GTCAGCAGGA CCACACCTCC CCTGACCACG CGCCAGCCCC CCACCACTGC CCCTAGTCGA
    GTGCCTGGAC GCCGTCCCCT GCCCCCAGGC ACCCAGCAGC CCCGAAGGCC CTGTGACTCC
    CAGCCCTGCC TCCATGGGGG GACCTGCCAG GACCAGGGCT CAGGTGCAGA CTTCACCTGC
    AGCTGCCCAG CAGGCACAGA GGGTGCCGTC TGTGAGAAGG CCCTGCACCC CTCCGTGCCA
    GCCTTCGGGG GCTCCTCCTT CCTGGCCTTT CCCACCCTTC GTGCCTACCA CACCCTGCGC
    CTGGCGCTCG AGTTCCGGGC GCTGGAGCCC CAGGGGCTGC TGCTCTACAA CGGCAATGCC
    CGGGGCAAGG ACTTCCTGGG ACTGGCGCTG CTGGGAGGCC GCGTGCAGTT CAGGTTCGAC
    ACAGGCTCAG GGCCCGCGGT GCTGACGAGC TCCGTGCCCG TGCAGCCCGG CCAGTGGCAT
    CGCCTGGAGC TGTCTCGGCA CTGGCGCCAA GGTACTCTGT CCGTGGATGG TGAGACTCCT
    GTCCTGGGCC AGAGCCCCAG TGGCACTGAT GGTCTCAACC TGGACACTGA CCTTTTCGTG
    GGTGGCGTCC CTGAGGATCA GGCTTCCACG GTGCTAGAGC GGACCTCTGT CAGCGTCGGT
    CTTAGGGGTT GTATCCGCTT GCTGGACGTC AACAATCAGC GGCTAGAGCT GAGCAGCTGG
    CCGGAATCTG CGACTCGAAA CTCCGGAGTG GGCGAGTGTG GGGACCACCC CTGTCTGCCT
    AGCCCCTGCC TCGGAGGGGC CCCGTGCCAG GCCCTGGAGG CAGGCAGGTT CCGCTGTCAG
    TGCCCACCCG GCCGCTTTGG CCCAACTTGT GCCGACGAGG ACCCTTGTCA GCCGAACCCC
    TGCCATGGAG CAGCCCCCTG CCGCGTGCTG CCTCAGGGAG AGGCCAAGTG CGAGTGCCCC
    CATGGCCGGG AGGGCAGCCT CTGCCAGACA GTCTCAGAGC CAGAGGACAA CCAGCCGTTC
    CTGGCTGATT TCAGCAGCTT CTCCTACCTG GAACTGAAAG GCCTGCACAC CTTTGAGCGG
    GACCTGGGGG AGAAGATGGC GCTGGAGGTG GTATTCCTGG CTCGGAGCCC CAGCGGCCTG
    CTCCTCTATA ATGGACAGAA GACAGATGGC AAGGGTGACT TTGTGTCTCT GGCGCTGCAC
    AATGGCCTCC TGGAGTTCCG CTACGACCTG GGCAAGGGGG CAGCGGTCAT CAGGAGCAAG
    GAGCCGGTGG CCCTGGGTGC TTGGACCAGG GTCTCCCTGG AGCGAAATGG ACGCAAAGGA
    GCCATGCGGG TTGGCGATGG GCCTCGCGTG CTGGGGGAGT CCCCGAAATC CCGCAAGGTG
    CCACACACCG TTCTCAACCT AAAGGAGCCA CTCTTTGTTG GGGGGGCCCC TGATTTCAGC
    AAGCTGGCCC GAGCAGCTGC CGTGTCCTCT GGCTTTGATG GAGCCATCCA GCTGGTCTCC
    CTGAATGGCC GCCAACTGCT GACCCGTGAG CATGTGGTCC GGGCCATGGA CGTCTCGTCC
    TTTGCAGACC ACCCTTGTAC CCAGGCCGAG GGTCAGCCTT GCCTCCATGG GGCTTCCTGT
    CTCCCCCGTG AGGCCTCCTA TGAGTGCCTG TGTCCTGCAG GCTTCTCGGG GCTGCACTGT
    GAGAAGGGCT TGATTGAGAA ATCAGCTGGG GACCTGGACG CACTGGCTTT TGATGGACGG
    ACCTACATCG AGTACCTCAA CGCTGTGACC GAGAGCGAAC TGACCAATGA GATCCCAGCT
    GAGAAGGCAC TCCAGAGCAA CCACTTTGAG CTGAGCCTGC GCACTGAGGC CACACAGGGG
    CTGGTGCTGT GGAGTGGCAA GGCCACGGAG CGGGCTGACT ACATCGCACT GGCCATCGTG
    GACGGGCGCC TGCAGCTGGC TTACGACCTG GGCTCCCAGC CCGTGGTGCT GCGCTCCACC
    GTACCAGTCA ATACCAACCG CTGGTTGCGG ATCAGGGCAC ACAGGAAACA GAGGGAAGGT
    TCCCTTCAGG TGGGCAACGA GGCCCCTGTG ACTGGCTCCT CCCCACTGGG TGCCACGCAG
    CTGGACACAG ATGGAGCCCT GTGGCTGGGT GGCCTGGGGA AGCTGCCCAC GGGCCAGGCC
    CTGCCCAAAG CCTATGGCAC AGGCTTTGTG GGCTGCCTGC GGGACGTGGT GGTGGGCCAG
    CGCCCGCTGC ACCTGCTGGA GGACGCCATC ACCAAGCCAG AGCTTCGGCC CTGCCCTGCC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61817
    Clone ID Related Accession (Same CDS sequence) XM_027975851.1
    Accession Version XM_027975851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product agrin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC GCAGGGTCTC CGTCCTGGTG 
    CGGTATTTCA TGATCCCCTG TAATGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC
    TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC
    CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TTGGCGCTGT CTGCGAGCCC
    AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCGCCTG CCCCAGCGTG
    GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGAG GCGCATCCGC CTGCTGCGCC GCGGGCCCTG CGGGACCCGG
    GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCCTC TGCGGATGGA
    CTGACAGCTA CGTGCTTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAACG CCCTGTGTGC
    GGCAGCGACG GCAGTGACTA CCCCAGTGAG TGCCAGCTCT TGCGCCAAGC CTGTGCCCAC
    CAAGAGAACG TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTCCCTCAGT
    GACCTGAGCC GTACCTGCCG TGTGAACCCG CGCACGCGGC AGCCGGAGAT GCTCCTGCGA
    CCAGAGAGCT GCCCTCCCCG TGGGACACCC GTGTGTGGGG ACGACGGGGT TACTTACGAT
    AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCGAGGCC TCCTCCTGCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC
    CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGTGACCGCG TCGTCTGTGA CGGGGCCTAC
    AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC
    GAGTGTCAGC AGCAACGTAG CATCCCCATG AAGCACCAGG GCCCGTGTGA CCAGCCCCCA
    TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCGACGT GTACTGTGAA GAATGGGGAA
    GCCGAGTGTG CATGTCACCA GGTGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT
    GGCGTCACGT ATGGCAGTGT GTGCGAACTG GAAGCTTCAG CCTGTGCCCT TAGGCGGGAG
    ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCTTG
    TGTGAAGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT
    GTGTGTGGCT CTGACGGGCG CACATATGCC AATGAATGTG AACTACACGT CCACGCCTGC
    ACACGTCAGA TCAGTCTGCA CGTGGCCTCA GCCAGTCCCT GCCAGACCTG TGGAGATACA
    GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCA TGTGTCCCCG GTGTGAGCGC
    CCTCCACCCG GCCCCGTGTG TGGCAGTGAT GGCGTGACCT ACGGCAGTTC ATGTGAGCTC
    CGTGAGGCTG CCTGTCAGCA GCAGACACAG ATCGAGGAAG CCCGGGTGGG GCCATGTGAG
    CAAGCTGAGT GTGGCTCCGG AGGCTCAGGT TCGGGAGAGG ACGGCGAGTG TGAGCCTGAA
    CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGATGGGCC ATGCGTCTGC
    GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC
    CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG
    GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCATTGC CACCTGCGAT TCCCCTGCAC
    TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTATGG CGGCACCTGT
    GACCCAGTCA CAGGCCAGTG CTCCTGCCGC CCGGGTGTGG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ATGGCCGGAG CGGCTGTACC
    CCCTGCAGCT GTGACCCCCG GGGCGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA
    TGCTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCT
    CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCAACCCA CGACTTGTGC TGAGATGCTT
    TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA
    CCCACCTGTC CAGCGGCTGA TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACTTATGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAGAGC
    TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCGCATC AGACATCCGC ATCTGTGGCC
    CCCTTAGCAC TTGACCTGAA CAAGGCACCA TTGCTGCCCC CCAGCGCCTT GCCCCTGGCT
    CCCAGCAGTA CCCGCTACAG CCAGCCCACT GCTCGAGCCT CATCACAACC TTGGACCATG
    GCCAGCATCC CCAGGACCAC CGCAAGGCCA GTGCTGACCA TGCCTCCCAT GGCCCCCTCC
    CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGCGGT
    GACGAGGAGC TGAGCGGTGA CTTGGAGGCC AGTGGGACTG GCTCAGGAGG ACTTGAGTCC
    CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC TTGCTACAAC
    TCACCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG CTGGAGCTCG AAGGGGTCGA GGGGCAAGAA
    CTGTTCTACA CGCCGGAGAT GGCGGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG
    AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAATTCAG ACGTTAAGAA GGACTTCCGA
    AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC
    TTTGACCCCA CCACAGCCTT CAGAGCGTCC GACGTGGGCC AGGCCCTGCT CCGGCAGCTC
    CAGGTGTCCA GGCGCAGATC CCTGGGCGTG AAGCGGCCTC TGCAGGAACA CGTGCGGTTC
    ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACAGCC
    AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC
    AGTCACACAA GCCGGCCTGT CAGCAGGACC ACACCTCCCC TGACCACGCG CCAGCCCCCC
    ACCACTGCCC CTAGTCGAGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC
    CGAAGGCCCT GTGACTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCA
    GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACAGAGG GTGCCGTCTG TGAGAAGGCC
    CTGCACCCCT CCGTGCCAGC CTTCGGGGGC TCCTCCTTCC TGGCCTTTCC CACCCTTCGT
    GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG
    CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGCGCTGCT GGGAGGCCGC
    GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACGAGCTC CGTGCCCGTG
    CAGCCCGGCC AGTGGCATCG CCTGGAGCTG TCTCGGCACT GGCGCCAAGG TACTCTGTCC
    GTGGATGGTG AGACTCCTGT CCTGGGCCAG AGCCCCAGTG GCACTGATGG TCTCAACCTG
    GACACTGACC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG
    ACCTCTGTCA GCGTCGGTCT TAGGGGTTGT ATCCGCTTGC TGGACGTCAA CAATCAGCGG
    CTAGAGCTGA GCAGCTGGCC GGAATCTGCG ACTCGAAACT CCGGAGTGGG CGAGTGTGGG
    GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA
    GGCAGGTTCC GCTGTCAGTG CCCACCCGGC CGCTTTGGCC CAACTTGTGC CGACGAGGAC
    CCTTGTCAGC CGAACCCCTG CCATGGAGCA GCCCCCTGCC GCGTGCTGCC TCAGGGAGAG
    GCCAAGTGCG AGTGCCCCCA TGGCCGGGAG GGCAGCCTCT GCCAGACAGT CTCAGAGCCA
    GAGGACAACC AGCCGTTCCT GGCTGATTTC AGCAGCTTCT CCTACCTGGA ACTGAAAGGC
    CTGCACACCT TTGAGCGGGA CCTGGGGGAG AAGATGGCGC TGGAGGTGGT ATTCCTGGCT
    CGGAGCCCCA GCGGCCTGCT CCTCTATAAT GGACAGAAGA CAGATGGCAA GGGTGACTTT
    GTGTCTCTGG CGCTGCACAA TGGCCTCCTG GAGTTCCGCT ACGACCTGGG CAAGGGGGCA
    GCGGTCATCA GGAGCAAGGA GCCGGTGGCC CTGGGTGCTT GGACCAGGGT CTCCCTGGAG
    CGAAATGGAC GCAAAGGAGC CATGCGGGTT GGCGATGGGC CTCGCGTGCT GGGGGAGTCC
    CCGAAATCCC GCAAGGTGCC ACACACCGTT CTCAACCTAA AGGAGCCACT CTTTGTTGGG
    GGGGCCCCTG ATTTCAGCAA GCTGGCCCGA GCAGCTGCCG TGTCCTCTGG CTTTGATGGA
    GCCATCCAGC TGGTCTCCCT GAATGGCCGC CAACTGCTGA CCCGTGAGCA TGTGGTCCGG
    GCCATGGACG TCTCGTCCTT TGCAGACCAC CCTTGTACCC AGGCCGAGGG TCAGCCTTGC
    CTCCATGGGG CTTCCTGTCT CCCCCGTGAG GCCTCCTATG AGTGCCTGTG TCCTGCAGGC
    TTCTCGGGGC TGCACTGTGA GAAGGGCTTG ATTGAGAAAT CAGCTGGGGA CCTGGACGCA
    CTGGCTTTTG ATGGACGGAC CTACATCGAG TACCTCAACG CTGTGACCGA GAGTGAGAAG
    GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG
    CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG
    CGCCTGCAGC TGGCTTACGA CCTGGGCTCC CAGCCCGTGG TGCTGCGCTC CACCGTACCA
    GTCAATACCA ACCGCTGGTT GCGGATCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT
    CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC
    ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GGGAAGCTGC CCACGGGCCA GGCCCTGCCC
    AAAGCCTATG GCACAGGCTT TGTGGGCTGC CTGCGGGACG TGGTGGTGGG CCAGCGCCCG
    CTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831652.1
    CDS323..6142
    Translation

    Target ORF information:

    RefSeq Version XM_027975851.1
    Organism Ovis aries(sheep)
    Definition Ovis aries agrin (AGRN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC GCAGGGTCTC CGTCCTGGTG 
    CGGTATTTCA TGATCCCCTG TAATGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC
    TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC
    CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TTGGCGCTGT CTGCGAGCCC
    AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCGCCTG CCCCAGCGTG
    GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG
    GCGCAGTGCA GCCAGCAGAG GCGCATCCGC CTGCTGCGCC GCGGGCCCTG CGGGACCCGG
    GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCCTC TGCGGATGGA
    CTGACAGCTA CGTGCTTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAACG CCCTGTGTGC
    GGCAGCGACG GCAGTGACTA CCCCAGTGAG TGCCAGCTCT TGCGCCAAGC CTGTGCCCAC
    CAAGAGAACG TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTCCCTCAGT
    GACCTGAGCC GTACCTGCCG TGTGAACCCG CGCACGCGGC AGCCGGAGAT GCTCCTGCGA
    CCAGAGAGCT GCCCTCCCCG TGGGACACCC GTGTGTGGGG ACGACGGGGT TACTTACGAT
    AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCGAGGCC TCCTCCTGCA GAAAGTGCGC
    TCTGGCCAGT GCCAGCCTCA AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC
    CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGTGACCGCG TCGTCTGTGA CGGGGCCTAC
    AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC
    GAGTGTCAGC AGCAACGTAG CATCCCCATG AAGCACCAGG GCCCGTGTGA CCAGCCCCCA
    TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCGACGT GTACTGTGAA GAATGGGGAA
    GCCGAGTGTG CATGTCACCA GGTGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT
    GGCGTCACGT ATGGCAGTGT GTGCGAACTG GAAGCTTCAG CCTGTGCCCT TAGGCGGGAG
    ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCTTG
    TGTGAAGCTG AGACTGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT
    GTGTGTGGCT CTGACGGGCG CACATATGCC AATGAATGTG AACTACACGT CCACGCCTGC
    ACACGTCAGA TCAGTCTGCA CGTGGCCTCA GCCAGTCCCT GCCAGACCTG TGGAGATACA
    GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCA TGTGTCCCCG GTGTGAGCGC
    CCTCCACCCG GCCCCGTGTG TGGCAGTGAT GGCGTGACCT ACGGCAGTTC ATGTGAGCTC
    CGTGAGGCTG CCTGTCAGCA GCAGACACAG ATCGAGGAAG CCCGGGTGGG GCCATGTGAG
    CAAGCTGAGT GTGGCTCCGG AGGCTCAGGT TCGGGAGAGG ACGGCGAGTG TGAGCCTGAA
    CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGATGGGCC ATGCGTCTGC
    GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC
    CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG
    GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCATTGC CACCTGCGAT TCCCCTGCAC
    TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTGGGC
    CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTATGG CGGCACCTGT
    GACCCAGTCA CAGGCCAGTG CTCCTGCCGC CCGGGTGTGG GGGGCCTCAA GTGTGACCGC
    TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ATGGCCGGAG CGGCTGTACC
    CCCTGCAGCT GTGACCCCCG GGGCGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA
    TGCTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCT
    CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCAACCCA CGACTTGTGC TGAGATGCTT
    TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA
    CCCACCTGTC CAGCGGCTGA TGCTACCAAG GTCTGTGGGT CAGATGGAGT CACTTATGGC
    AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAGAGC
    TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCGCATC AGACATCCGC ATCTGTGGCC
    CCCTTAGCAC TTGACCTGAA CAAGGCACCA TTGCTGCCCC CCAGCGCCTT GCCCCTGGCT
    CCCAGCAGTA CCCGCTACAG CCAGCCCACT GCTCGAGCCT CATCACAACC TTGGACCATG
    GCCAGCATCC CCAGGACCAC CGCAAGGCCA GTGCTGACCA TGCCTCCCAT GGCCCCCTCC
    CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGCGGT
    GACGAGGAGC TGAGCGGTGA CTTGGAGGCC AGTGGGACTG GCTCAGGAGG ACTTGAGTCC
    CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC TTGCTACAAC
    TCACCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC
    TGTCCTGCCA CCAAGGCGTT CCAGGGTGTG CTGGAGCTCG AAGGGGTCGA GGGGCAAGAA
    CTGTTCTACA CGCCGGAGAT GGCGGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG
    AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAATTCAG ACGTTAAGAA GGACTTCCGA
    AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC
    TTTGACCCCA CCACAGCCTT CAGAGCGTCC GACGTGGGCC AGGCCCTGCT CCGGCAGCTC
    CAGGTGTCCA GGCGCAGATC CCTGGGCGTG AAGCGGCCTC TGCAGGAACA CGTGCGGTTC
    ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACAGCC
    AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC
    AGTCACACAA GCCGGCCTGT CAGCAGGACC ACACCTCCCC TGACCACGCG CCAGCCCCCC
    ACCACTGCCC CTAGTCGAGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC
    CGAAGGCCCT GTGACTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCA
    GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACAGAGG GTGCCGTCTG TGAGAAGGCC
    CTGCACCCCT CCGTGCCAGC CTTCGGGGGC TCCTCCTTCC TGGCCTTTCC CACCCTTCGT
    GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG
    CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGCGCTGCT GGGAGGCCGC
    GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACGAGCTC CGTGCCCGTG
    CAGCCCGGCC AGTGGCATCG CCTGGAGCTG TCTCGGCACT GGCGCCAAGG TACTCTGTCC
    GTGGATGGTG AGACTCCTGT CCTGGGCCAG AGCCCCAGTG GCACTGATGG TCTCAACCTG
    GACACTGACC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG
    ACCTCTGTCA GCGTCGGTCT TAGGGGTTGT ATCCGCTTGC TGGACGTCAA CAATCAGCGG
    CTAGAGCTGA GCAGCTGGCC GGAATCTGCG ACTCGAAACT CCGGAGTGGG CGAGTGTGGG
    GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA
    GGCAGGTTCC GCTGTCAGTG CCCACCCGGC CGCTTTGGCC CAACTTGTGC CGACGAGGAC
    CCTTGTCAGC CGAACCCCTG CCATGGAGCA GCCCCCTGCC GCGTGCTGCC TCAGGGAGAG
    GCCAAGTGCG AGTGCCCCCA TGGCCGGGAG GGCAGCCTCT GCCAGACAGT CTCAGAGCCA
    GAGGACAACC AGCCGTTCCT GGCTGATTTC AGCAGCTTCT CCTACCTGGA ACTGAAAGGC
    CTGCACACCT TTGAGCGGGA CCTGGGGGAG AAGATGGCGC TGGAGGTGGT ATTCCTGGCT
    CGGAGCCCCA GCGGCCTGCT CCTCTATAAT GGACAGAAGA CAGATGGCAA GGGTGACTTT
    GTGTCTCTGG CGCTGCACAA TGGCCTCCTG GAGTTCCGCT ACGACCTGGG CAAGGGGGCA
    GCGGTCATCA GGAGCAAGGA GCCGGTGGCC CTGGGTGCTT GGACCAGGGT CTCCCTGGAG
    CGAAATGGAC GCAAAGGAGC CATGCGGGTT GGCGATGGGC CTCGCGTGCT GGGGGAGTCC
    CCGAAATCCC GCAAGGTGCC ACACACCGTT CTCAACCTAA AGGAGCCACT CTTTGTTGGG
    GGGGCCCCTG ATTTCAGCAA GCTGGCCCGA GCAGCTGCCG TGTCCTCTGG CTTTGATGGA
    GCCATCCAGC TGGTCTCCCT GAATGGCCGC CAACTGCTGA CCCGTGAGCA TGTGGTCCGG
    GCCATGGACG TCTCGTCCTT TGCAGACCAC CCTTGTACCC AGGCCGAGGG TCAGCCTTGC
    CTCCATGGGG CTTCCTGTCT CCCCCGTGAG GCCTCCTATG AGTGCCTGTG TCCTGCAGGC
    TTCTCGGGGC TGCACTGTGA GAAGGGCTTG ATTGAGAAAT CAGCTGGGGA CCTGGACGCA
    CTGGCTTTTG ATGGACGGAC CTACATCGAG TACCTCAACG CTGTGACCGA GAGTGAGAAG
    GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG
    CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG
    CGCCTGCAGC TGGCTTACGA CCTGGGCTCC CAGCCCGTGG TGCTGCGCTC CACCGTACCA
    GTCAATACCA ACCGCTGGTT GCGGATCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT
    CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC
    ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GGGAAGCTGC CCACGGGCCA GGCCCTGCCC
    AAAGCCTATG GCACAGGCTT TGTGGGCTGC CTGCGGGACG TGGTGGTGGG CCAGCGCCCG
    CTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv18898
    Clone ID Related Accession (Same CDS sequence) XM_012138572.2
    Accession Version XM_012138572.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCTG AGAAGGCACT CCAGAGCAAC
    CACTTTGAGC TGAGCCTGCG CACTGAGGCC ACACAGGGGC TGGTGCTGTG GAGTGGCAAG
    GCCACGGAGC GGGCTGACTA CATCGCACTG GCCATCGTGG ACGGGCGCCT GCAGCTGGCT
    TACGACCTGG GCTCCCAGCC CGTGGTGCTG CGCTCCACCG TACCAGTCAA TACCAACCGC
    TGGTTGCGGA TCAGGGCACA CAGGAAACAG AGGGAAGGTT CCCTTCAGGT GGGCAACGAG
    GCCCCTGTGA CTGGCTCCTC CCCACTGGGT GCCACGCAGC TGGACACAGA TGGAGCCCTG
    TGGCTGGGTG GCCTGGGGAA GCTGCCCACG GGCCAGGCCC TGCCCAAAGC CTATGGCACA
    GGCTTTGTGG GCTGCCTGCG GGACGTGGTG GTGGGCCAGC GCCCGCTGCA CCTGCTGGAG
    GACGCCATCA CCAAGCCAGA GCTTCGGCCC TGCCCTGCCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993962.1
    CDS111..6275
    Translation

    Target ORF information:

    RefSeq Version XM_012138572.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138572.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCTG AGAAGGCACT CCAGAGCAAC
    CACTTTGAGC TGAGCCTGCG CACTGAGGCC ACACAGGGGC TGGTGCTGTG GAGTGGCAAG
    GCCACGGAGC GGGCTGACTA CATCGCACTG GCCATCGTGG ACGGGCGCCT GCAGCTGGCT
    TACGACCTGG GCTCCCAGCC CGTGGTGCTG CGCTCCACCG TACCAGTCAA TACCAACCGC
    TGGTTGCGGA TCAGGGCACA CAGGAAACAG AGGGAAGGTT CCCTTCAGGT GGGCAACGAG
    GCCCCTGTGA CTGGCTCCTC CCCACTGGGT GCCACGCAGC TGGACACAGA TGGAGCCCTG
    TGGCTGGGTG GCCTGGGGAA GCTGCCCACG GGCCAGGCCC TGCCCAAAGC CTATGGCACA
    GGCTTTGTGG GCTGCCTGCG GGACGTGGTG GTGGGCCAGC GCCCGCTGCA CCTGCTGGAG
    GACGCCATCA CCAAGCCAGA GCTTCGGCCC TGCCCTGCCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv18899
    Clone ID Related Accession (Same CDS sequence) XM_012138573.2
    Accession Version XM_012138573.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGGTGCCA CACACCGTTC TCAACCTAAA GGAGCCACTC
    TTTGTTGGGG GGGCCCCTGA TTTCAGCAAG CTGGCCCGAG CAGCTGCCGT GTCCTCTGGC
    TTTGATGGAG CCATCCAGCT GGTCTCCCTG AATGGCCGCC AACTGCTGAC CCGTGAGCAT
    GTGGTCCGGG CCATGGACGT CTCGTCCTTT GCAGACCACC CTTGTACCCA GGCCGAGGGT
    CAGCCTTGCC TCCATGGGGC TTCCTGTCTC CCCCGTGAGG CCTCCTATGA GTGCCTGTGT
    CCTGCAGGCT TCTCGGGGCT GCACTGTGAG AAGGGCTTGA TTGAGAAATC AGCTGGGGAC
    CTGGACGCAC TGGCTTTTGA TGGACGGACC TACATCGAGT ACCTCAACGC TGTGACCGAG
    AGCGAACTGA CCAATGAGAT CCCAGCTGAG AAGGCACTCC AGAGCAACCA CTTTGAGCTG
    AGCCTGCGCA CTGAGGCCAC ACAGGGGCTG GTGCTGTGGA GTGGCAAGGC CACGGAGCGG
    GCTGACTACA TCGCACTGGC CATCGTGGAC GGGCGCCTGC AGCTGGCTTA CGACCTGGGC
    TCCCAGCCCG TGGTGCTGCG CTCCACCGTA CCAGTCAATA CCAACCGCTG GTTGCGGATC
    AGGGCACACA GGAAACAGAG GGAAGGTTCC CTTCAGGTGG GCAACGAGGC CCCTGTGACT
    GGCTCCTCCC CACTGGGTGC CACGCAGCTG GACACAGATG GAGCCCTGTG GCTGGGTGGC
    CTGGGGAAGC TGCCCACGGG CCAGGCCCTG CCCAAAGCCT ATGGCACAGG CTTTGTGGGC
    TGCCTGCGGG ACGTGGTGGT GGGCCAGCGC CCGCTGCACC TGCTGGAGGA CGCCATCACC
    AAGCCAGAGC TTCGGCCCTG CCCTGCCCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993963.1
    CDS110..6262
    Translation

    Target ORF information:

    RefSeq Version XM_012138573.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138573.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGGTGCCA CACACCGTTC TCAACCTAAA GGAGCCACTC
    TTTGTTGGGG GGGCCCCTGA TTTCAGCAAG CTGGCCCGAG CAGCTGCCGT GTCCTCTGGC
    TTTGATGGAG CCATCCAGCT GGTCTCCCTG AATGGCCGCC AACTGCTGAC CCGTGAGCAT
    GTGGTCCGGG CCATGGACGT CTCGTCCTTT GCAGACCACC CTTGTACCCA GGCCGAGGGT
    CAGCCTTGCC TCCATGGGGC TTCCTGTCTC CCCCGTGAGG CCTCCTATGA GTGCCTGTGT
    CCTGCAGGCT TCTCGGGGCT GCACTGTGAG AAGGGCTTGA TTGAGAAATC AGCTGGGGAC
    CTGGACGCAC TGGCTTTTGA TGGACGGACC TACATCGAGT ACCTCAACGC TGTGACCGAG
    AGCGAACTGA CCAATGAGAT CCCAGCTGAG AAGGCACTCC AGAGCAACCA CTTTGAGCTG
    AGCCTGCGCA CTGAGGCCAC ACAGGGGCTG GTGCTGTGGA GTGGCAAGGC CACGGAGCGG
    GCTGACTACA TCGCACTGGC CATCGTGGAC GGGCGCCTGC AGCTGGCTTA CGACCTGGGC
    TCCCAGCCCG TGGTGCTGCG CTCCACCGTA CCAGTCAATA CCAACCGCTG GTTGCGGATC
    AGGGCACACA GGAAACAGAG GGAAGGTTCC CTTCAGGTGG GCAACGAGGC CCCTGTGACT
    GGCTCCTCCC CACTGGGTGC CACGCAGCTG GACACAGATG GAGCCCTGTG GCTGGGTGGC
    CTGGGGAAGC TGCCCACGGG CCAGGCCCTG CCCAAAGCCT ATGGCACAGG CTTTGTGGGC
    TGCCTGCGGG ACGTGGTGGT GGGCCAGCGC CCGCTGCACC TGCTGGAGGA CGCCATCACC
    AAGCCAGAGC TTCGGCCCTG CCCTGCCCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv18900
    Clone ID Related Accession (Same CDS sequence) XM_012138574.2
    Accession Version XM_012138574.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT
    GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC
    GCACTGGCCA TCGTGGACGG GCGCCTGCAG CTGGCTTACG ACCTGGGCTC CCAGCCCGTG
    GTGCTGCGCT CCACCGTACC AGTCAATACC AACCGCTGGT TGCGGATCAG GGCACACAGG
    AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA
    CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGGGAAGCTG
    CCCACGGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TTGTGGGCTG CCTGCGGGAC
    GTGGTGGTGG GCCAGCGCCC GCTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT
    CGGCCCTGCC CTGCCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993964.1
    CDS110..6250
    Translation

    Target ORF information:

    RefSeq Version XM_012138574.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138574.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT
    GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC
    GCACTGGCCA TCGTGGACGG GCGCCTGCAG CTGGCTTACG ACCTGGGCTC CCAGCCCGTG
    GTGCTGCGCT CCACCGTACC AGTCAATACC AACCGCTGGT TGCGGATCAG GGCACACAGG
    AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA
    CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGGGAAGCTG
    CCCACGGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TTGTGGGCTG CCTGCGGGAC
    GTGGTGGTGG GCCAGCGCCC GCTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT
    CGGCCCTGCC CTGCCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv18901
    Clone ID Related Accession (Same CDS sequence) XM_012138575.2
    Accession Version XM_012138575.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGGTGCCA CACACCGTTC TCAACCTAAA GGAGCCACTC
    TTTGTTGGGG GGGCCCCTGA TTTCAGCAAG CTGGCCCGAG CAGCTGCCGT GTCCTCTGGC
    TTTGATGGAG CCATCCAGCT GGTCTCCCTG AATGGCCGCC AACTGCTGAC CCGTGAGCAT
    GTGGTCCGGG CCATGGACGT CTCGTCCTTT GCAGACCACC CTTGTACCCA GGCCGAGGGT
    CAGCCTTGCC TCCATGGGGC TTCCTGTCTC CCCCGTGAGG CCTCCTATGA GTGCCTGTGT
    CCTGCAGGCT TCTCGGGGCT GCACTGTGAG AAGGGCTTGA TTGAGAAATC AGCTGGGGAC
    CTGGACGCAC TGGCTTTTGA TGGACGGACC TACATCGAGT ACCTCAACGC TGTGACCGAG
    AGTGAGAAGG CACTCCAGAG CAACCACTTT GAGCTGAGCC TGCGCACTGA GGCCACACAG
    GGGCTGGTGC TGTGGAGTGG CAAGGCCACG GAGCGGGCTG ACTACATCGC ACTGGCCATC
    GTGGACGGGC GCCTGCAGCT GGCTTACGAC CTGGGCTCCC AGCCCGTGGT GCTGCGCTCC
    ACCGTACCAG TCAATACCAA CCGCTGGTTG CGGATCAGGG CACACAGGAA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA CGAGGCCCCT GTGACTGGCT CCTCCCCACT GGGTGCCACG
    CAGCTGGACA CAGATGGAGC CCTGTGGCTG GGTGGCCTGG GGAAGCTGCC CACGGGCCAG
    GCCCTGCCCA AAGCCTATGG CACAGGCTTT GTGGGCTGCC TGCGGGACGT GGTGGTGGGC
    CAGCGCCCGC TGCACCTGCT GGAGGACGCC ATCACCAAGC CAGAGCTTCG GCCCTGCCCT
    GCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993965.1
    CDS110..6238
    Translation

    Target ORF information:

    RefSeq Version XM_012138575.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138575.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGGTGCCA CACACCGTTC TCAACCTAAA GGAGCCACTC
    TTTGTTGGGG GGGCCCCTGA TTTCAGCAAG CTGGCCCGAG CAGCTGCCGT GTCCTCTGGC
    TTTGATGGAG CCATCCAGCT GGTCTCCCTG AATGGCCGCC AACTGCTGAC CCGTGAGCAT
    GTGGTCCGGG CCATGGACGT CTCGTCCTTT GCAGACCACC CTTGTACCCA GGCCGAGGGT
    CAGCCTTGCC TCCATGGGGC TTCCTGTCTC CCCCGTGAGG CCTCCTATGA GTGCCTGTGT
    CCTGCAGGCT TCTCGGGGCT GCACTGTGAG AAGGGCTTGA TTGAGAAATC AGCTGGGGAC
    CTGGACGCAC TGGCTTTTGA TGGACGGACC TACATCGAGT ACCTCAACGC TGTGACCGAG
    AGTGAGAAGG CACTCCAGAG CAACCACTTT GAGCTGAGCC TGCGCACTGA GGCCACACAG
    GGGCTGGTGC TGTGGAGTGG CAAGGCCACG GAGCGGGCTG ACTACATCGC ACTGGCCATC
    GTGGACGGGC GCCTGCAGCT GGCTTACGAC CTGGGCTCCC AGCCCGTGGT GCTGCGCTCC
    ACCGTACCAG TCAATACCAA CCGCTGGTTG CGGATCAGGG CACACAGGAA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA CGAGGCCCCT GTGACTGGCT CCTCCCCACT GGGTGCCACG
    CAGCTGGACA CAGATGGAGC CCTGTGGCTG GGTGGCCTGG GGAAGCTGCC CACGGGCCAG
    GCCCTGCCCA AAGCCTATGG CACAGGCTTT GTGGGCTGCC TGCGGGACGT GGTGGTGGGC
    CAGCGCCCGC TGCACCTGCT GGAGGACGCC ATCACCAAGC CAGAGCTTCG GCCCTGCCCT
    GCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv18907
    Clone ID Related Accession (Same CDS sequence) XM_012138582.2
    Accession Version XM_012138582.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product agrin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942440.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012138582.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCCC CTGAAACTCT GGATTCCGGG
    GCCCGTCCCA GTGAGAAGGC ACTCCAGAGC AACCACTTTG AGCTGAGCCT GCGCACTGAG
    GCCACACAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACGG AGCGGGCTGA CTACATCGCA
    CTGGCCATCG TGGACGGGCG CCTGCAGCTG GCTTACGACC TGGGCTCCCA GCCCGTGGTG
    CTGCGCTCCA CCGTACCAGT CAATACCAAC CGCTGGTTGC GGATCAGGGC ACACAGGAAA
    CAGAGGGAAG GTTCCCTTCA GGTGGGCAAC GAGGCCCCTG TGACTGGCTC CTCCCCACTG
    GGTGCCACGC AGCTGGACAC AGATGGAGCC CTGTGGCTGG GTGGCCTGGG GAAGCTGCCC
    ACGGGCCAGG CCCTGCCCAA AGCCTATGGC ACAGGCTTTG TGGGCTGCCT GCGGGACGTG
    GTGGTGGGCC AGCGCCCGCT GCACCTGCTG GAGGACGCCA TCACCAAGCC AGAGCTTCGG
    CCCTGCCCTG CCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011993972.1
    CDS111..6308
    Translation

    Target ORF information:

    RefSeq Version XM_012138582.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon agrin (AGRN), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012138582.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCCC CTGAAACTCT GGATTCCGGG
    GCCCGTCCCA GTGAGAAGGC ACTCCAGAGC AACCACTTTG AGCTGAGCCT GCGCACTGAG
    GCCACACAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACGG AGCGGGCTGA CTACATCGCA
    CTGGCCATCG TGGACGGGCG CCTGCAGCTG GCTTACGACC TGGGCTCCCA GCCCGTGGTG
    CTGCGCTCCA CCGTACCAGT CAATACCAAC CGCTGGTTGC GGATCAGGGC ACACAGGAAA
    CAGAGGGAAG GTTCCCTTCA GGTGGGCAAC GAGGCCCCTG TGACTGGCTC CTCCCCACTG
    GGTGCCACGC AGCTGGACAC AGATGGAGCC CTGTGGCTGG GTGGCCTGGG GAAGCTGCCC
    ACGGGCCAGG CCCTGCCCAA AGCCTATGGC ACAGGCTTTG TGGGCTGCCT GCGGGACGTG
    GTGGTGGGCC AGCGCCCGCT GCACCTGCTG GAGGACGCCA TCACCAAGCC AGAGCTTCGG
    CCCTGCCCTG CCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm51778
    Clone ID Related Accession (Same CDS sequence) XM_015099069.1
    Accession Version XM_015099069.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product LOW QUALITY PROTEIN: agrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639021.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GAGTGCGGCT CGGACGCCTC CACCTACAGC
    CAGCGAGTGC GACTGCAGGC GGGCCAGTGA GCCCCAGGCA GCAGGCGCAT CCGCCTGCTG
    CGCCGCGGGC CCACAGGGAC CCGGGACCCC TGCTCCAATG TCACCTGCAG CTTTGGCAGC
    ACCTGTGTGC CCTCTGCGGA TGGACTGACA GCTACGTGCT TGTGCCCTGC CACGTGCCTG
    GGGGCCCCGG AACGCCCTGT GTGCGGCAGC GACGGCAGTG ACTACCCCAG TGAGTGCCAG
    CTCTTGCGCC AAGCCTGTGC CCACCAAGAG AACGTCTTCA AGAAGTTCGA CGGTCCTTGC
    GACCCCTGCC AGGGCTCCCT CAGTGACCTG AGCCGTACCT GCCGTGTGAA CCCGCGCACG
    CGGCAGCCGG AGATGCTCCT GCGACCAGAG AGCTGCCCTC CCCGTGGGAC ACCCGTGTGT
    GGGGACGACG GGGTTACTTA CGATAATGAC TGCGTCATGG GCCGAACAGG GGCTGCTCGA
    GGCCTCCTCC TGCAGAAAGT GCGCTCTGGC CAGTGCCAGC CTCAAGACCA GTGCCCGGAG
    CCGTGCAGAT TCAATGCCGT CTGCCTAAGC ACCAGGGCCC GTGTGGTGAG CATGCGGGTG
    GGCTCCGTGC CGGGAGCAGC TGCTGGCAGG ATGAGGTACC GCGCCTGCCG CCAGGAGCCC
    ACAGCCCGAG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC TGACGTGGGC
    CGGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CCCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCCC CTGAAACTCT GGATTCCGGG
    GCCCGTCCCA GTGAGAAGGC ACTCCAGAGC AACCACTTTG AGCTGAGCCT GCGCACTGAG
    GCCACACAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACGG AGCGGGCTGA CTACATCGCA
    CTGGCCATCG TGGACGGGCG CCTGCAGCTG GCTTACGACC TGGGCTCCCA GCCCGTGGTG
    CTGCGCTCCA CCGTACCAGT CAATACCAAC CGCTGGTTGC GGATCAGGGC ACACAGGAAA
    CAGAGGGAAG GTTCCCTTCA GGTGGGCAAC GAGGCCCCTG TGACTGGCTC CTCCCCACTG
    GGTGCCACGC AGCTGGACAC AGATGGAGCC CTGTGGCTGG GTGGCCTGGG GAAGCTGCCC
    ACGGGCCAGG CCCTGCCCAA AGCCTATGGC ACAGGCTTTG TGGGCTGCCT GCGGGACGTG
    GTGGTGGGCC AGCGCCCGCT GCACCTGCTG GAGGACGCCA TCACCAAGCC AGAGCTTCGG
    CCCTGCCCTG CCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014954555.1
    CDS48..6185
    Translation

    Target ORF information:

    RefSeq Version XM_015099069.1
    Organism Ovis aries(sheep)
    Definition Ovis aries agrin (AGRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015099069.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GAGTGCGGCT CGGACGCCTC CACCTACAGC
    CAGCGAGTGC GACTGCAGGC GGGCCAGTGA GCCCCAGGCA GCAGGCGCAT CCGCCTGCTG
    CGCCGCGGGC CCACAGGGAC CCGGGACCCC TGCTCCAATG TCACCTGCAG CTTTGGCAGC
    ACCTGTGTGC CCTCTGCGGA TGGACTGACA GCTACGTGCT TGTGCCCTGC CACGTGCCTG
    GGGGCCCCGG AACGCCCTGT GTGCGGCAGC GACGGCAGTG ACTACCCCAG TGAGTGCCAG
    CTCTTGCGCC AAGCCTGTGC CCACCAAGAG AACGTCTTCA AGAAGTTCGA CGGTCCTTGC
    GACCCCTGCC AGGGCTCCCT CAGTGACCTG AGCCGTACCT GCCGTGTGAA CCCGCGCACG
    CGGCAGCCGG AGATGCTCCT GCGACCAGAG AGCTGCCCTC CCCGTGGGAC ACCCGTGTGT
    GGGGACGACG GGGTTACTTA CGATAATGAC TGCGTCATGG GCCGAACAGG GGCTGCTCGA
    GGCCTCCTCC TGCAGAAAGT GCGCTCTGGC CAGTGCCAGC CTCAAGACCA GTGCCCGGAG
    CCGTGCAGAT TCAATGCCGT CTGCCTAAGC ACCAGGGCCC GTGTGGTGAG CATGCGGGTG
    GGCTCCGTGC CGGGAGCAGC TGCTGGCAGG ATGAGGTACC GCGCCTGCCG CCAGGAGCCC
    ACAGCCCGAG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC TGACGTGGGC
    CGGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CCCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCCC CTGAAACTCT GGATTCCGGG
    GCCCGTCCCA GTGAGAAGGC ACTCCAGAGC AACCACTTTG AGCTGAGCCT GCGCACTGAG
    GCCACACAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACGG AGCGGGCTGA CTACATCGCA
    CTGGCCATCG TGGACGGGCG CCTGCAGCTG GCTTACGACC TGGGCTCCCA GCCCGTGGTG
    CTGCGCTCCA CCGTACCAGT CAATACCAAC CGCTGGTTGC GGATCAGGGC ACACAGGAAA
    CAGAGGGAAG GTTCCCTTCA GGTGGGCAAC GAGGCCCCTG TGACTGGCTC CTCCCCACTG
    GGTGCCACGC AGCTGGACAC AGATGGAGCC CTGTGGCTGG GTGGCCTGGG GAAGCTGCCC
    ACGGGCCAGG CCCTGCCCAA AGCCTATGGC ACAGGCTTTG TGGGCTGCCT GCGGGACGTG
    GTGGTGGGCC AGCGCCCGCT GCACCTGCTG GAGGACGCCA TCACCAAGCC AGAGCTTCGG
    CCCTGCCCTG CCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61810
    Clone ID Related Accession (Same CDS sequence) XM_027975844.1
    Accession Version XM_027975844.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product agrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCTG AGAAGGCACT CCAGAGCAAC
    CACTTTGAGC TGAGCCTGCG CACTGAGGCC ACACAGGGGC TGGTGCTGTG GAGTGGCAAG
    GCCACGGAGC GGGCTGACTA CATCGCACTG GCCATCGTGG ACGGGCGCCT GCAGCTGGCT
    TACGACCTGG GCTCCCAGCC CGTGGTGCTG CGCTCCACCG TACCAGTCAA TACCAACCGC
    TGGTTGCGGA TCAGGGCACA CAGGAAACAG AGGGAAGGTT CCCTTCAGGT GGGCAACGAG
    GCCCCTGTGA CTGGCTCCTC CCCACTGGGT GCCACGCAGC TGGACACAGA TGGAGCCCTG
    TGGCTGGGTG GCCTGGGGAA GCTGCCCACG GGCCAGGCCC TGCCCAAAGC CTATGGCACA
    GGCTTTGTGG GCTGCCTGCG GGACGTGGTG GTGGGCCAGC GCCCGCTGCA CCTGCTGGAG
    GACGCCATCA CCAAGCCAGA GCTTCGGCCC TGCCCTGCCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831645.1
    CDS44..6208
    Translation

    Target ORF information:

    RefSeq Version XM_027975844.1
    Organism Ovis aries(sheep)
    Definition Ovis aries agrin (AGRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975844.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCTG AGAAGGCACT CCAGAGCAAC
    CACTTTGAGC TGAGCCTGCG CACTGAGGCC ACACAGGGGC TGGTGCTGTG GAGTGGCAAG
    GCCACGGAGC GGGCTGACTA CATCGCACTG GCCATCGTGG ACGGGCGCCT GCAGCTGGCT
    TACGACCTGG GCTCCCAGCC CGTGGTGCTG CGCTCCACCG TACCAGTCAA TACCAACCGC
    TGGTTGCGGA TCAGGGCACA CAGGAAACAG AGGGAAGGTT CCCTTCAGGT GGGCAACGAG
    GCCCCTGTGA CTGGCTCCTC CCCACTGGGT GCCACGCAGC TGGACACAGA TGGAGCCCTG
    TGGCTGGGTG GCCTGGGGAA GCTGCCCACG GGCCAGGCCC TGCCCAAAGC CTATGGCACA
    GGCTTTGTGG GCTGCCTGCG GGACGTGGTG GTGGGCCAGC GCCCGCTGCA CCTGCTGGAG
    GACGCCATCA CCAAGCCAGA GCTTCGGCCC TGCCCTGCCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61811
    Clone ID Related Accession (Same CDS sequence) XM_027975845.1
    Accession Version XM_027975845.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product agrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGGTGCCA CACACCGTTC TCAACCTAAA GGAGCCACTC
    TTTGTTGGGG GGGCCCCTGA TTTCAGCAAG CTGGCCCGAG CAGCTGCCGT GTCCTCTGGC
    TTTGATGGAG CCATCCAGCT GGTCTCCCTG AATGGCCGCC AACTGCTGAC CCGTGAGCAT
    GTGGTCCGGG CCATGGACGT CTCGTCCTTT GCAGACCACC CTTGTACCCA GGCCGAGGGT
    CAGCCTTGCC TCCATGGGGC TTCCTGTCTC CCCCGTGAGG CCTCCTATGA GTGCCTGTGT
    CCTGCAGGCT TCTCGGGGCT GCACTGTGAG AAGGGCTTGA TTGAGAAATC AGCTGGGGAC
    CTGGACGCAC TGGCTTTTGA TGGACGGACC TACATCGAGT ACCTCAACGC TGTGACCGAG
    AGCGAACTGA CCAATGAGAT CCCAGCTGAG AAGGCACTCC AGAGCAACCA CTTTGAGCTG
    AGCCTGCGCA CTGAGGCCAC ACAGGGGCTG GTGCTGTGGA GTGGCAAGGC CACGGAGCGG
    GCTGACTACA TCGCACTGGC CATCGTGGAC GGGCGCCTGC AGCTGGCTTA CGACCTGGGC
    TCCCAGCCCG TGGTGCTGCG CTCCACCGTA CCAGTCAATA CCAACCGCTG GTTGCGGATC
    AGGGCACACA GGAAACAGAG GGAAGGTTCC CTTCAGGTGG GCAACGAGGC CCCTGTGACT
    GGCTCCTCCC CACTGGGTGC CACGCAGCTG GACACAGATG GAGCCCTGTG GCTGGGTGGC
    CTGGGGAAGC TGCCCACGGG CCAGGCCCTG CCCAAAGCCT ATGGCACAGG CTTTGTGGGC
    TGCCTGCGGG ACGTGGTGGT GGGCCAGCGC CCGCTGCACC TGCTGGAGGA CGCCATCACC
    AAGCCAGAGC TTCGGCCCTG CCCTGCCCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831646.1
    CDS44..6196
    Translation

    Target ORF information:

    RefSeq Version XM_027975845.1
    Organism Ovis aries(sheep)
    Definition Ovis aries agrin (AGRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGGTGCCA CACACCGTTC TCAACCTAAA GGAGCCACTC
    TTTGTTGGGG GGGCCCCTGA TTTCAGCAAG CTGGCCCGAG CAGCTGCCGT GTCCTCTGGC
    TTTGATGGAG CCATCCAGCT GGTCTCCCTG AATGGCCGCC AACTGCTGAC CCGTGAGCAT
    GTGGTCCGGG CCATGGACGT CTCGTCCTTT GCAGACCACC CTTGTACCCA GGCCGAGGGT
    CAGCCTTGCC TCCATGGGGC TTCCTGTCTC CCCCGTGAGG CCTCCTATGA GTGCCTGTGT
    CCTGCAGGCT TCTCGGGGCT GCACTGTGAG AAGGGCTTGA TTGAGAAATC AGCTGGGGAC
    CTGGACGCAC TGGCTTTTGA TGGACGGACC TACATCGAGT ACCTCAACGC TGTGACCGAG
    AGCGAACTGA CCAATGAGAT CCCAGCTGAG AAGGCACTCC AGAGCAACCA CTTTGAGCTG
    AGCCTGCGCA CTGAGGCCAC ACAGGGGCTG GTGCTGTGGA GTGGCAAGGC CACGGAGCGG
    GCTGACTACA TCGCACTGGC CATCGTGGAC GGGCGCCTGC AGCTGGCTTA CGACCTGGGC
    TCCCAGCCCG TGGTGCTGCG CTCCACCGTA CCAGTCAATA CCAACCGCTG GTTGCGGATC
    AGGGCACACA GGAAACAGAG GGAAGGTTCC CTTCAGGTGG GCAACGAGGC CCCTGTGACT
    GGCTCCTCCC CACTGGGTGC CACGCAGCTG GACACAGATG GAGCCCTGTG GCTGGGTGGC
    CTGGGGAAGC TGCCCACGGG CCAGGCCCTG CCCAAAGCCT ATGGCACAGG CTTTGTGGGC
    TGCCTGCGGG ACGTGGTGGT GGGCCAGCGC CCGCTGCACC TGCTGGAGGA CGCCATCACC
    AAGCCAGAGC TTCGGCCCTG CCCTGCCCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61812
    Clone ID Related Accession (Same CDS sequence) XM_027975846.1
    Accession Version XM_027975846.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product agrin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT
    GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC
    GCACTGGCCA TCGTGGACGG GCGCCTGCAG CTGGCTTACG ACCTGGGCTC CCAGCCCGTG
    GTGCTGCGCT CCACCGTACC AGTCAATACC AACCGCTGGT TGCGGATCAG GGCACACAGG
    AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA
    CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGGGAAGCTG
    CCCACGGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TTGTGGGCTG CCTGCGGGAC
    GTGGTGGTGG GCCAGCGCCC GCTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT
    CGGCCCTGCC CTGCCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831647.1
    CDS43..6183
    Translation

    Target ORF information:

    RefSeq Version XM_027975846.1
    Organism Ovis aries(sheep)
    Definition Ovis aries agrin (AGRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT
    GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC
    GCACTGGCCA TCGTGGACGG GCGCCTGCAG CTGGCTTACG ACCTGGGCTC CCAGCCCGTG
    GTGCTGCGCT CCACCGTACC AGTCAATACC AACCGCTGGT TGCGGATCAG GGCACACAGG
    AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA
    CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGGGAAGCTG
    CCCACGGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TTGTGGGCTG CCTGCGGGAC
    GTGGTGGTGG GCCAGCGCCC GCTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT
    CGGCCCTGCC CTGCCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61813
    Clone ID Related Accession (Same CDS sequence) XM_027975847.1
    Accession Version XM_027975847.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product agrin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGGTGCCA CACACCGTTC TCAACCTAAA GGAGCCACTC
    TTTGTTGGGG GGGCCCCTGA TTTCAGCAAG CTGGCCCGAG CAGCTGCCGT GTCCTCTGGC
    TTTGATGGAG CCATCCAGCT GGTCTCCCTG AATGGCCGCC AACTGCTGAC CCGTGAGCAT
    GTGGTCCGGG CCATGGACGT CTCGTCCTTT GCAGACCACC CTTGTACCCA GGCCGAGGGT
    CAGCCTTGCC TCCATGGGGC TTCCTGTCTC CCCCGTGAGG CCTCCTATGA GTGCCTGTGT
    CCTGCAGGCT TCTCGGGGCT GCACTGTGAG AAGGGCTTGA TTGAGAAATC AGCTGGGGAC
    CTGGACGCAC TGGCTTTTGA TGGACGGACC TACATCGAGT ACCTCAACGC TGTGACCGAG
    AGTGAGAAGG CACTCCAGAG CAACCACTTT GAGCTGAGCC TGCGCACTGA GGCCACACAG
    GGGCTGGTGC TGTGGAGTGG CAAGGCCACG GAGCGGGCTG ACTACATCGC ACTGGCCATC
    GTGGACGGGC GCCTGCAGCT GGCTTACGAC CTGGGCTCCC AGCCCGTGGT GCTGCGCTCC
    ACCGTACCAG TCAATACCAA CCGCTGGTTG CGGATCAGGG CACACAGGAA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA CGAGGCCCCT GTGACTGGCT CCTCCCCACT GGGTGCCACG
    CAGCTGGACA CAGATGGAGC CCTGTGGCTG GGTGGCCTGG GGAAGCTGCC CACGGGCCAG
    GCCCTGCCCA AAGCCTATGG CACAGGCTTT GTGGGCTGCC TGCGGGACGT GGTGGTGGGC
    CAGCGCCCGC TGCACCTGCT GGAGGACGCC ATCACCAAGC CAGAGCTTCG GCCCTGCCCT
    GCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831648.1
    CDS42..6170
    Translation

    Target ORF information:

    RefSeq Version XM_027975847.1
    Organism Ovis aries(sheep)
    Definition Ovis aries agrin (AGRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGGTGCCA CACACCGTTC TCAACCTAAA GGAGCCACTC
    TTTGTTGGGG GGGCCCCTGA TTTCAGCAAG CTGGCCCGAG CAGCTGCCGT GTCCTCTGGC
    TTTGATGGAG CCATCCAGCT GGTCTCCCTG AATGGCCGCC AACTGCTGAC CCGTGAGCAT
    GTGGTCCGGG CCATGGACGT CTCGTCCTTT GCAGACCACC CTTGTACCCA GGCCGAGGGT
    CAGCCTTGCC TCCATGGGGC TTCCTGTCTC CCCCGTGAGG CCTCCTATGA GTGCCTGTGT
    CCTGCAGGCT TCTCGGGGCT GCACTGTGAG AAGGGCTTGA TTGAGAAATC AGCTGGGGAC
    CTGGACGCAC TGGCTTTTGA TGGACGGACC TACATCGAGT ACCTCAACGC TGTGACCGAG
    AGTGAGAAGG CACTCCAGAG CAACCACTTT GAGCTGAGCC TGCGCACTGA GGCCACACAG
    GGGCTGGTGC TGTGGAGTGG CAAGGCCACG GAGCGGGCTG ACTACATCGC ACTGGCCATC
    GTGGACGGGC GCCTGCAGCT GGCTTACGAC CTGGGCTCCC AGCCCGTGGT GCTGCGCTCC
    ACCGTACCAG TCAATACCAA CCGCTGGTTG CGGATCAGGG CACACAGGAA ACAGAGGGAA
    GGTTCCCTTC AGGTGGGCAA CGAGGCCCCT GTGACTGGCT CCTCCCCACT GGGTGCCACG
    CAGCTGGACA CAGATGGAGC CCTGTGGCTG GGTGGCCTGG GGAAGCTGCC CACGGGCCAG
    GCCCTGCCCA AAGCCTATGG CACAGGCTTT GTGGGCTGCC TGCGGGACGT GGTGGTGGGC
    CAGCGCCCGC TGCACCTGCT GGAGGACGCC ATCACCAAGC CAGAGCTTCG GCCCTGCCCT
    GCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm61816
    Clone ID Related Accession (Same CDS sequence) XM_027975850.1
    Accession Version XM_027975850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product agrin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCCC CTGAAACTCT GGATTCCGGG
    GCCCGTCCCA GTGAGAAGGC ACTCCAGAGC AACCACTTTG AGCTGAGCCT GCGCACTGAG
    GCCACACAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACGG AGCGGGCTGA CTACATCGCA
    CTGGCCATCG TGGACGGGCG CCTGCAGCTG GCTTACGACC TGGGCTCCCA GCCCGTGGTG
    CTGCGCTCCA CCGTACCAGT CAATACCAAC CGCTGGTTGC GGATCAGGGC ACACAGGAAA
    CAGAGGGAAG GTTCCCTTCA GGTGGGCAAC GAGGCCCCTG TGACTGGCTC CTCCCCACTG
    GGTGCCACGC AGCTGGACAC AGATGGAGCC CTGTGGCTGG GTGGCCTGGG GAAGCTGCCC
    ACGGGCCAGG CCCTGCCCAA AGCCTATGGC ACAGGCTTTG TGGGCTGCCT GCGGGACGTG
    GTGGTGGGCC AGCGCCCGCT GCACCTGCTG GAGGACGCCA TCACCAAGCC AGAGCTTCGG
    CCCTGCCCTG CCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027831651.1
    CDS45..6242
    Translation

    Target ORF information:

    RefSeq Version XM_027975850.1
    Organism Ovis aries(sheep)
    Definition Ovis aries agrin (AGRN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027975850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCAGCC TGCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
    GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG
    CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC
    CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT
    GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA
    GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC
    CCTGCCCCAC CATACCTGTG GCCGGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC
    ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG
    ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC
    TTTGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG
    AAGAGCGCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC
    AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG CCTGCTGCGC
    CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC
    TGTGTGCCCT CTGCGGATGG ACTGACAGCT ACGTGCTTGT GCCCTGCCAC GTGCCTGGGG
    GCCCCGGAAC GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGTGA GTGCCAGCTC
    TTGCGCCAAG CCTGTGCCCA CCAAGAGAAC GTCTTCAAGA AGTTCGACGG TCCTTGCGAC
    CCCTGCCAGG GCTCCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC GCGCACGCGG
    CAGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC CGTGTGTGGG
    GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCGAGGC
    CTCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC AAGACCAGTG CCCGGAGCCG
    TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGTGACCGC
    GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT
    GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAACGTA GCATCCCCAT GAAGCACCAG
    GGCCCGTGTG ACCAGCCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCGACG
    TGTACTGTGA AGAATGGGGA AGCCGAGTGT GCATGTCACC AGGTGTGTTC AGGCGTCTAT
    GACCCTGTGT GTGGCAGTGA TGGCGTCACG TATGGCAGTG TGTGCGAACT GGAAGCTTCA
    GCCTGTGCCC TTAGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG
    CAGTGCCGCT TTGGAGCCTT GTGTGAAGCT GAGACTGGGC GCTGTGTGTG CCCCTCCGAA
    TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGACGGGC GCACATATGC CAATGAATGT
    GAACTACACG TCCACGCCTG CACACGTCAG ATCAGTCTGC ACGTGGCCTC AGCCAGTCCC
    TGCCAGACCT GTGGAGATAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC
    ATGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGTGA TGGCGTGACC
    TACGGCAGTT CATGTGAGCT CCGTGAGGCT GCCTGTCAGC AGCAGACACA GATCGAGGAA
    GCCCGGGTGG GGCCATGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCAGG TTCGGGAGAG
    GACGGCGAGT GTGAGCCTGA ACTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG
    GAGGATGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT
    GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA
    CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCATTG
    CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC
    ACTGTTGCCC AGGGTGTGGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC
    GGCTCCTATG GCGGCACCTG TGACCCAGTC ACAGGCCAGT GCTCCTGCCG CCCGGGTGTG
    GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT
    GATGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGCGCCGT GCGGGACGAC
    TGTGAGCAGA TGACTGGGCT ATGCTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG
    CAGTGTCCAG ATGGCCGTGC TCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCAACCC
    ACGACTTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT
    GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGCGGCTG ATGCTACCAA GGTCTGTGGG
    TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC
    TTGGAGATCT CTATCCAGAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCGCAT
    CAGACATCCG CATCTGTGGC CCCCTTAGCA CTTGACCTGA ACAAGGCACC ATTGCTGCCC
    CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCTACA GCCAGCCCAC TGCTCGAGCC
    TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CCGCAAGGCC AGTGCTGACC
    ATGCCTCCCA TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT
    GGCAGCGCTG ATGGGAGCGG TGACGAGGAG CTGAGCGGTG ACTTGGAGGC CAGTGGGACT
    GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG
    GAAAGGGCTT CTTGCTACAA CTCACCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC
    CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGCGT TCCAGGGTGT GCTGGAGCTC
    GAAGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCGGACCC CAAGTCTGAG
    CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAATTCA
    GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC
    CGGGCCATTG TGGAAGTGCA CTTTGACCCC ACCACAGCCT TCAGAGCGTC CGACGTGGGC
    CAGGCCCTGC TCCGGCAGCT CCAGGTGTCC AGGCGCAGAT CCCTGGGCGT GAAGCGGCCT
    CTGCAGGAAC ACGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC
    ACCCCAGCTG CAGCCACAGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA
    ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCTG TCAGCAGGAC CACACCTCCC
    CTGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGAG TGCCTGGACG CCGTCCCCTG
    CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGACTCCC AGCCCTGCCT CCATGGGGGG
    ACCTGCCAGG ACCAGGGCTC AGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACAGAG
    GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTCGGGGG CTCCTCCTTC
    CTGGCCTTTC CCACCCTTCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG
    CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA
    CTGGCGCTGC TGGGAGGCCG CGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG
    CTGACGAGCT CCGTGCCCGT GCAGCCCGGC CAGTGGCATC GCCTGGAGCT GTCTCGGCAC
    TGGCGCCAAG GTACTCTGTC CGTGGATGGT GAGACTCCTG TCCTGGGCCA GAGCCCCAGT
    GGCACTGATG GTCTCAACCT GGACACTGAC CTTTTCGTGG GTGGCGTCCC TGAGGATCAG
    GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCGTCGGTC TTAGGGGTTG TATCCGCTTG
    CTGGACGTCA ACAATCAGCG GCTAGAGCTG AGCAGCTGGC CGGAATCTGC GACTCGAAAC
    TCCGGAGTGG GCGAGTGTGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC
    CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CGCTGTCAGT GCCCACCCGG CCGCTTTGGC
    CCAACTTGTG CCGACGAGGA CCCTTGTCAG CCGAACCCCT GCCATGGAGC AGCCCCCTGC
    CGCGTGCTGC CTCAGGGAGA GGCCAAGTGC GAGTGCCCCC ATGGCCGGGA GGGCAGCCTC
    TGCCAGACAG TCTCAGAGCC AGAGGACAAC CAGCCGTTCC TGGCTGATTT CAGCAGCTTC
    TCCTACCTGG AACTGAAAGG CCTGCACACC TTTGAGCGGG ACCTGGGGGA GAAGATGGCG
    CTGGAGGTGG TATTCCTGGC TCGGAGCCCC AGCGGCCTGC TCCTCTATAA TGGACAGAAG
    ACAGATGGCA AGGGTGACTT TGTGTCTCTG GCGCTGCACA ATGGCCTCCT GGAGTTCCGC
    TACGACCTGG GCAAGGGGGC AGCGGTCATC AGGAGCAAGG AGCCGGTGGC CCTGGGTGCT
    TGGACCAGGG TCTCCCTGGA GCGAAATGGA CGCAAAGGAG CCATGCGGGT TGGCGATGGG
    CCTCGCGTGC TGGGGGAGTC CCCGAAATCC CGCAAGGTGC CACACACCGT TCTCAACCTA
    AAGGAGCCAC TCTTTGTTGG GGGGGCCCCT GATTTCAGCA AGCTGGCCCG AGCAGCTGCC
    GTGTCCTCTG GCTTTGATGG AGCCATCCAG CTGGTCTCCC TGAATGGCCG CCAACTGCTG
    ACCCGTGAGC ATGTGGTCCG GGCCATGGAC GTCTCGTCCT TTGCAGACCA CCCTTGTACC
    CAGGCCGAGG GTCAGCCTTG CCTCCATGGG GCTTCCTGTC TCCCCCGTGA GGCCTCCTAT
    GAGTGCCTGT GTCCTGCAGG CTTCTCGGGG CTGCACTGTG AGAAGGGCTT GATTGAGAAA
    TCAGCTGGGG ACCTGGACGC ACTGGCTTTT GATGGACGGA CCTACATCGA GTACCTCAAC
    GCTGTGACCG AGAGCGAACT GACCAATGAG ATCCCAGCCC CTGAAACTCT GGATTCCGGG
    GCCCGTCCCA GTGAGAAGGC ACTCCAGAGC AACCACTTTG AGCTGAGCCT GCGCACTGAG
    GCCACACAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACGG AGCGGGCTGA CTACATCGCA
    CTGGCCATCG TGGACGGGCG CCTGCAGCTG GCTTACGACC TGGGCTCCCA GCCCGTGGTG
    CTGCGCTCCA CCGTACCAGT CAATACCAAC CGCTGGTTGC GGATCAGGGC ACACAGGAAA
    CAGAGGGAAG GTTCCCTTCA GGTGGGCAAC GAGGCCCCTG TGACTGGCTC CTCCCCACTG
    GGTGCCACGC AGCTGGACAC AGATGGAGCC CTGTGGCTGG GTGGCCTGGG GAAGCTGCCC
    ACGGGCCAGG CCCTGCCCAA AGCCTATGGC ACAGGCTTTG TGGGCTGCCT GCGGGACGTG
    GTGGTGGGCC AGCGCCCGCT GCACCTGCTG GAGGACGCCA TCACCAAGCC AGAGCTTCGG
    CCCTGCCCTG CCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.