PLCB4 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_015099525.1	XP_014955011.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012188107.2	XP_012043497.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_004014147.3	XP_004014196.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X5
XM_004014148.3	XP_004014197.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X7
XM_004014146.3	XP_004014195.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X8
XM_012188111.2	XP_012043501.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X9
XM_012162893.1	XP_012018283.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012162902.2	XP_012018292.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
XM_012188100.2	XP_012043490.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_015099526.1	XP_014955012.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012188102.2	XP_012043492.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012188105.2	XP_012043495.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_012188109.2	XP_012043499.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X4
XM_012162887.2	XP_012018277.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012162889.2	XP_012018279.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012162892.1	XP_012018282.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012162896.2	XP_012018286.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012162897.2	XP_012018287.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_012162900.2	XP_012018290.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_012162903.2	XP_012018293.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X4
XM_012162905.2	XP_012018295.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X6
XM_012162907.2	XP_012018297.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X8
XM_027976469.1	XP_027832270.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
XM_012162891.2	XP_012018281.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012162898.2	XP_012018288.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_012162904.2	XP_012018294.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X5
XM_012162906.2	XP_012018296.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X7
XM_012188108.2	XP_012043498.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
XM_012188104.2	XP_012043494.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_012162886.2	XP_012018276.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012162888.2	XP_012018278.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012162890.1	XP_012018280.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012162908.2	XP_012018298.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X9
XM_012162899.1	XP_012018289.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_012162901.1	XP_012018291.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_012188101.2	XP_012043491.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_012188106.2	XP_012043496.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_012188110.2	XP_012043500.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv34185	XM_012162888.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34185	XM_012162889.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34185	XM_012162886.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34185	XM_012162887.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34185	XM_012162890.1 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34185	XM_012162891.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34185	XM_012162892.1 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34185	XM_012162893.1 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34185	XM_012162896.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34186	XM_012162901.1 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34186	XM_012162897.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34186	XM_012162900.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34186	XM_012162898.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34186	XM_012162899.1 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34187	XM_012162902.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34188	XM_012162903.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34189	XM_012162904.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34190	XM_012162905.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34191	XM_012162906.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34192	XM_012162907.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv34193	XM_012162908.2 Latest version!	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSm62516	XM_027976469.1 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm27692	XM_012188100.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27692	XM_015099526.1 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27692	XM_012188101.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27692	XM_012188102.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27692	XM_015099525.1 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27693	XM_012188104.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27693	XM_012188107.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27693	XM_012188106.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27693	XM_012188105.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27694	XM_012188108.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27695	XM_012188109.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27696	XM_004014147.3 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27697	XM_012188110.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27698	XM_004014148.3 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27699	XM_004014146.3 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27700	XM_012188111.2 Latest version!	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

PLCB4 ( XM_012162893.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018283.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOv34185
Clone ID Related Accession (Same CDS sequence)	XM_012162893.1 , XM_012162887.2 , XM_012162889.2 , XM_012162892.1 , XM_012162896.2 , XM_012162891.2 , XM_012162886.2 , XM_012162888.2 , XM_012162890.1
Accession Version	XM_012162893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018283.1
CDS	2860..6519
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162893.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162893.1

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162887.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018277.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OOv34185
Clone ID Related Accession (Same CDS sequence)	XM_012162893.1 , XM_012162887.2 , XM_012162889.2 , XM_012162892.1 , XM_012162896.2 , XM_012162891.2 , XM_012162886.2 , XM_012162888.2 , XM_012162890.1
Accession Version	XM_012162887.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018277.1
CDS	508..4167
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162887.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162887.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162889.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018279.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv34185
Clone ID Related Accession (Same CDS sequence)	XM_012162893.1 , XM_012162887.2 , XM_012162889.2 , XM_012162892.1 , XM_012162896.2 , XM_012162891.2 , XM_012162886.2 , XM_012162888.2 , XM_012162890.1
Accession Version	XM_012162889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018279.1
CDS	501..4160
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162889.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162889.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162892.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018282.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA.

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CloneID	OOv34185
Clone ID Related Accession (Same CDS sequence)	XM_012162893.1 , XM_012162887.2 , XM_012162889.2 , XM_012162892.1 , XM_012162896.2 , XM_012162891.2 , XM_012162886.2 , XM_012162888.2 , XM_012162890.1
Accession Version	XM_012162892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018282.1
CDS	1464..5123
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162892.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162892.1

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162896.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018286.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OOv34185
Clone ID Related Accession (Same CDS sequence)	XM_012162893.1 , XM_012162887.2 , XM_012162889.2 , XM_012162892.1 , XM_012162896.2 , XM_012162891.2 , XM_012162886.2 , XM_012162888.2 , XM_012162890.1
Accession Version	XM_012162896.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162896.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018286.1
CDS	233..3892
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162896.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162896.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162891.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018281.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OOv34185
Clone ID Related Accession (Same CDS sequence)	XM_012162893.1 , XM_012162887.2 , XM_012162889.2 , XM_012162892.1 , XM_012162896.2 , XM_012162891.2 , XM_012162886.2 , XM_012162888.2 , XM_012162890.1
Accession Version	XM_012162891.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018281.1
CDS	582..4241
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162891.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162891.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162886.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018276.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X17, mRNA.

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CloneID	OOv34185
Clone ID Related Accession (Same CDS sequence)	XM_012162893.1 , XM_012162887.2 , XM_012162889.2 , XM_012162892.1 , XM_012162896.2 , XM_012162891.2 , XM_012162886.2 , XM_012162888.2 , XM_012162890.1
Accession Version	XM_012162886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018276.1
CDS	345..4004
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162886.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162886.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162888.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018278.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OOv34185
Clone ID Related Accession (Same CDS sequence)	XM_012162893.1 , XM_012162887.2 , XM_012162889.2 , XM_012162892.1 , XM_012162896.2 , XM_012162891.2 , XM_012162886.2 , XM_012162888.2 , XM_012162890.1
Accession Version	XM_012162888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018278.1
CDS	419..4078
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162888.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162888.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162890.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018280.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv34185
Clone ID Related Accession (Same CDS sequence)	XM_012162893.1 , XM_012162887.2 , XM_012162889.2 , XM_012162892.1 , XM_012162896.2 , XM_012162891.2 , XM_012162886.2 , XM_012162888.2 , XM_012162890.1
Accession Version	XM_012162890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018280.1
CDS	1301..4960
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162890.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162890.1

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162897.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018287.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OOv34186
Clone ID Related Accession (Same CDS sequence)	XM_012162897.2 , XM_012162900.2 , XM_012162898.2 , XM_012162899.1 , XM_012162901.1
Accession Version	XM_012162897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018287.1
CDS	345..3986
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162897.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162897.2

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162900.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018290.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OOv34186
Clone ID Related Accession (Same CDS sequence)	XM_012162897.2 , XM_012162900.2 , XM_012162898.2 , XM_012162899.1 , XM_012162901.1
Accession Version	XM_012162900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018290.1
CDS	286..3927
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162900.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162900.2

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162898.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018288.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X23, mRNA.

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CloneID	OOv34186
Clone ID Related Accession (Same CDS sequence)	XM_012162897.2 , XM_012162900.2 , XM_012162898.2 , XM_012162899.1 , XM_012162901.1
Accession Version	XM_012162898.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018288.1
CDS	508..4149
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162898.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162898.2

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162899.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018289.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOv34186
Clone ID Related Accession (Same CDS sequence)	XM_012162897.2 , XM_012162900.2 , XM_012162898.2 , XM_012162899.1 , XM_012162901.1
Accession Version	XM_012162899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018289.1
CDS	2860..6501
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162899.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162899.1

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162901.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018291.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OOv34186
Clone ID Related Accession (Same CDS sequence)	XM_012162897.2 , XM_012162900.2 , XM_012162898.2 , XM_012162899.1 , XM_012162901.1
Accession Version	XM_012162901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018291.1
CDS	36..3677
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162901.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162901.1

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAATGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CCATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGCGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162902.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018292.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X26, mRNA.

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CloneID	OOv34187
Clone ID Related Accession (Same CDS sequence)	XM_012162902.2
Accession Version	XM_012162902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTATCGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA 
AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAATTCTTTA 
AAGAAGAAAC ATGCAAAGGA ACACAGCACC ATGCAGAAGC TACACTGCAC GCAAGTTGAC 
AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCATG AGAAAATCCT CGAGAAGGCG 
ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT 
CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG 
GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGGTCCG GGACCTGCAC 
CTCAGCCAGC AATGCGAACT GCTCAGAAAG CTGCTGATCA GTGCCCACGA GCAGCAGACC 
CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGCGCCCA CCAAGCTAAG 
ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA 
CGGGAAAGGA GAGTCAGGGA GTTAAATAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA 
AAGAGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTGGAG 
CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA TGCAGTGTCC 
CAAACTCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG 
ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018292.1
CDS	344..3982
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162902.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162902.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTATCGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA 
AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAATTCTTTA 
AAGAAGAAAC ATGCAAAGGA ACACAGCACC ATGCAGAAGC TACACTGCAC GCAAGTTGAC 
AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCATG AGAAAATCCT CGAGAAGGCG 
ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT 
CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG 
GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGGTCCG GGACCTGCAC 
CTCAGCCAGC AATGCGAACT GCTCAGAAAG CTGCTGATCA GTGCCCACGA GCAGCAGACC 
CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGCGCCCA CCAAGCTAAG 
ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA 
CGGGAAAGGA GAGTCAGGGA GTTAAATAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA 
AAGAGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTGGAG 
CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA TGCAGTGTCC 
CAAACTCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG 
ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162903.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018293.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OOv34188
Clone ID Related Accession (Same CDS sequence)	XM_012162903.2
Accession Version	XM_012162903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCATAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGATACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGAG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACGTA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAATGGTC TCAATCCCGT TTACAATGAA 
GAGTCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAC CACCATAAGC 
GTGGGCGGTA TCGAACTGAT CCCTCAAGTG AGGATAGAAG ATTTAAAGCA GATGAAGGCT 
TACTTGAAGC ATTTAAAGAA ACAACAGAAA GAGCTCAATT CTTTAAAGAA GAAACATGCA 
AAGGAACACA GCACCATGCA GAAGCTACAC TGCACGCAAG TTGACAAGAT TGTGGCACAG 
TACGACAAGG AGAAATTGAC TCATGAGAAA ATCCTCGAGA AGGCGATGAA GAAGAAAGGG 
GGAAGTAATT GTCTTGAAAT GAAGAAAGAA ACAGAAATTA AAATTCAGAC GCTGACATCA 
GATCACAAAT CTAAGGTCAA GGAGATTGTG GCACAGCACA CAAAGGAGTG GTCAGACATG 
ATCAACACCC ACAGTGCGGA GGAGCAGGAG GTCCGGGACC TGCACCTCAG CCAGCAATGC 
GAACTGCTCA GAAAGCTGCT GATCAGTGCC CACGAGCAGC AGACCCAGCA GCTGAAACTG 
TCCCATGACA GGGAAAGCAA AGAGATGCGC GCCCACCAAG CTAAGATTTC TATGGAAAAT 
AGCAAAGCCA TCAGCCAAGA TAAATCCATC AAGAATAAAG CAGAACGGGA AAGGAGAGTC 
AGGGAGTTAA ATAGCAGCAA CACTAAAAAG TTTCTGGAAG AAAGAAAGAG ACTCGCCATG 
AAGCAGTCCA AAGAAATGGA TCAGTTGAAA AAAGTCCAGC TGGAGCACCT CGAATTCCTA 
GAGAAACAGA ATGAGCAGCT TTTGAAATCC TGTCATGCAG TGTCCCAAAC TCAAGGCGAA 
GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC CGCAGACCAG CAACAGTTGT 
GTGAAACTCC AGGACACAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018293.1
CDS	345..3968
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162903.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162903.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCATAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGATACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGAG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACGTA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAATGGTC TCAATCCCGT TTACAATGAA 
GAGTCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAC CACCATAAGC 
GTGGGCGGTA TCGAACTGAT CCCTCAAGTG AGGATAGAAG ATTTAAAGCA GATGAAGGCT 
TACTTGAAGC ATTTAAAGAA ACAACAGAAA GAGCTCAATT CTTTAAAGAA GAAACATGCA 
AAGGAACACA GCACCATGCA GAAGCTACAC TGCACGCAAG TTGACAAGAT TGTGGCACAG 
TACGACAAGG AGAAATTGAC TCATGAGAAA ATCCTCGAGA AGGCGATGAA GAAGAAAGGG 
GGAAGTAATT GTCTTGAAAT GAAGAAAGAA ACAGAAATTA AAATTCAGAC GCTGACATCA 
GATCACAAAT CTAAGGTCAA GGAGATTGTG GCACAGCACA CAAAGGAGTG GTCAGACATG 
ATCAACACCC ACAGTGCGGA GGAGCAGGAG GTCCGGGACC TGCACCTCAG CCAGCAATGC 
GAACTGCTCA GAAAGCTGCT GATCAGTGCC CACGAGCAGC AGACCCAGCA GCTGAAACTG 
TCCCATGACA GGGAAAGCAA AGAGATGCGC GCCCACCAAG CTAAGATTTC TATGGAAAAT 
AGCAAAGCCA TCAGCCAAGA TAAATCCATC AAGAATAAAG CAGAACGGGA AAGGAGAGTC 
AGGGAGTTAA ATAGCAGCAA CACTAAAAAG TTTCTGGAAG AAAGAAAGAG ACTCGCCATG 
AAGCAGTCCA AAGAAATGGA TCAGTTGAAA AAAGTCCAGC TGGAGCACCT CGAATTCCTA 
GAGAAACAGA ATGAGCAGCT TTTGAAATCC TGTCATGCAG TGTCCCAAAC TCAAGGCGAA 
GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC CGCAGACCAG CAACAGTTGT 
GTGAAACTCC AGGACACAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162904.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018294.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OOv34189
Clone ID Related Accession (Same CDS sequence)	XM_012162904.2
Accession Version	XM_012162904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCATAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGATACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGAG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACGTA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAATGGTC TCAATCCCGT TTACAATGAA 
GAGTCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT CGAACTGATC 
CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA 
CAACAGAAAG AGCTCAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG CACCATGCAG 
AAGCTACACT GCACGCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT 
CATGAGAAAA TCCTCGAGAA GGCGATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG 
AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG 
GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG 
GAGCAGGAGG TCCGGGACCT GCACCTCAGC CAGCAATGCG AACTGCTCAG AAAGCTGCTG 
ATCAGTGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA 
GAGATGCGCG CCCACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT 
AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC 
ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT 
CAGTTGAAAA AAGTCCAGCT GGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGCTT 
TTGAAATCCT GTCATGCAGT GTCCCAAACT CAAGGCGAAG GAGATGCAGC AGATGGTGAA 
ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG TGAAACTCCA GGACACAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018294.1
CDS	344..3946
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162904.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162904.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCATAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGATACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGAG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACGTA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAATGGTC TCAATCCCGT TTACAATGAA 
GAGTCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT CGAACTGATC 
CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA 
CAACAGAAAG AGCTCAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG CACCATGCAG 
AAGCTACACT GCACGCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT 
CATGAGAAAA TCCTCGAGAA GGCGATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG 
AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG 
GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG 
GAGCAGGAGG TCCGGGACCT GCACCTCAGC CAGCAATGCG AACTGCTCAG AAAGCTGCTG 
ATCAGTGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA 
GAGATGCGCG CCCACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT 
AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC 
ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT 
CAGTTGAAAA AAGTCCAGCT GGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGCTT 
TTGAAATCCT GTCATGCAGT GTCCCAAACT CAAGGCGAAG GAGATGCAGC AGATGGTGAA 
ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG TGAAACTCCA GGACACAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162905.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018295.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X29, mRNA.

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CloneID	OOv34190
Clone ID Related Accession (Same CDS sequence)	XM_012162905.2
Accession Version	XM_012162905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGGCGAAG 
GAGATGCAGC AGATGGTGAA ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018295.1
CDS	345..3947
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV

Target ORF information:

RefSeq Version	XM_012162905.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162905.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACC ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGCGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGGCGAAG 
GAGATGCAGC AGATGGTGAA ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162906.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018296.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OOv34191
Clone ID Related Accession (Same CDS sequence)	XM_012162906.2
Accession Version	XM_012162906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTATCGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA 
AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAATTCTTTA 
AAGAAGAAAC ATGCAAAGGA ACACAGCACC ATGCAGAAGC TACACTGCAC GCAAGTTGAC 
AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCATG AGAAAATCCT CGAGAAGGCG 
ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT 
CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG 
GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGGTCCG GGACCTGCAC 
CTCAGCCAGC AATGCGAACT GCTCAGAAAG CTGCTGATCA GTGCCCACGA GCAGCAGACC 
CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGCGCCCA CCAAGCTAAG 
ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA 
CGGGAAAGGA GAGTCAGGGA GTTAAATAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA 
AAGAGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTGGAG 
CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGGCGAAGG AGATGCAGCA GATGGTGAAA 
TTGGAAGCCG AGATGGACCG CAGACCAGCA ACAGTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018296.1
CDS	345..3926
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV

Target ORF information:

RefSeq Version	XM_012162906.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162906.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCATATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGA TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGAGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACGTATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ATGGTCTCAA TCCCGTTTAC AATGAAGAGT CATTTGTGTT TCGGAAGGTG 
ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTATCGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA 
AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAATTCTTTA 
AAGAAGAAAC ATGCAAAGGA ACACAGCACC ATGCAGAAGC TACACTGCAC GCAAGTTGAC 
AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCATG AGAAAATCCT CGAGAAGGCG 
ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT 
CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG 
GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGGTCCG GGACCTGCAC 
CTCAGCCAGC AATGCGAACT GCTCAGAAAG CTGCTGATCA GTGCCCACGA GCAGCAGACC 
CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGCGCCCA CCAAGCTAAG 
ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA 
CGGGAAAGGA GAGTCAGGGA GTTAAATAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA 
AAGAGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTGGAG 
CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGGCGAAGG AGATGCAGCA GATGGTGAAA 
TTGGAAGCCG AGATGGACCG CAGACCAGCA ACAGTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162907.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018297.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OOv34192
Clone ID Related Accession (Same CDS sequence)	XM_012162907.2
Accession Version	XM_012162907.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCATAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGATACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGAG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACGTA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAATGGTC TCAATCCCGT TTACAATGAA 
GAGTCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT CGAACTGATC 
CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA 
CAACAGAAAG AGCTCAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG CACCATGCAG 
AAGCTACACT GCACGCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT 
CATGAGAAAA TCCTCGAGAA GGCGATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG 
AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG 
GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG 
GAGCAGGAGG TCCGGGACCT GCACCTCAGC CAGCAATGCG AACTGCTCAG AAAGCTGCTG 
ATCAGTGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA 
GAGATGCGCG CCCACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT 
AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC 
ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT 
CAGTTGAAAA AAGTCCAGCT GGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGGCG 
AAGGAGATGC AGCAGATGGT GAAATTGGAA GCCGAGATGG ACCGCAGACC AGCAACAGTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018297.1
CDS	345..3890
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV

Target ORF information:

RefSeq Version	XM_012162907.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162907.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCATAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGATACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGAG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACGTA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAATGGTC TCAATCCCGT TTACAATGAA 
GAGTCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT CGAACTGATC 
CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA 
CAACAGAAAG AGCTCAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG CACCATGCAG 
AAGCTACACT GCACGCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT 
CATGAGAAAA TCCTCGAGAA GGCGATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG 
AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG 
GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG 
GAGCAGGAGG TCCGGGACCT GCACCTCAGC CAGCAATGCG AACTGCTCAG AAAGCTGCTG 
ATCAGTGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA 
GAGATGCGCG CCCACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT 
AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC 
ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT 
CAGTTGAAAA AAGTCCAGCT GGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGGCG 
AAGGAGATGC AGCAGATGGT GAAATTGGAA GCCGAGATGG ACCGCAGACC AGCAACAGTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012162908.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012018298.1 Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OOv34193
Clone ID Related Accession (Same CDS sequence)	XM_012162908.2
Accession Version	XM_012162908.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTGCC TCAAGAAGCA CTGGATGAAA TTGGCATTTA TGACCAACAC AAATGGGAAA 
ATTCCAGTTA GGAGTATTAC TAGAACCTTT GCATCGGGGA AAACAGAAAA GGTGATCTTT 
CAAGCGCTCA AGGAGCTAGG TCTTCCCAGT GGAAAGAATG ATGAAATTGA GCCTGCCGCA 
TTTACTTATG AAAAGTTCTA TGAACTGACA CAAAAGATTT GTCCTCGGAC AGATATAGAG 
GATCTTTTCA AAAAAATCAA TGGAGACAAA ACTGATTATT TAACGGTAGA CCAATTAGTG 
AGCTTTCTAA ATGAACATCA ACGAGATCCT CGACTGAATG AAATTTTATT CCCATTTTAT 
GATGCTAAAA GGGCAATGCA GATCATTGAG ATGTATGAGC CTGATGAAGA TTTGAAGAAA 
CAAGGCCTCA TATCAAGTGA TGGGTTTTGC AGATACCTGA TGTCAGATGA AAATGCCCCC 
GTCTTCCTAG ATCGTCTAGA GCTGTACCAA GAAATGGACC ACCCTCTGGC TCACTACTTC 
ATCAGCTCTT CCCACAACAC CTATCTCACT GGCAGACAGT TTGGTGGGAA ATCTTCAGTA 
GAAATGTACA GACAGGTCCT CCTGGCTGGT TGCAGATGTG TTGAACTTGA CTGTTGGGAT 
GGAAAAGGTG AGGACCAAGA ACCGATAATA ACTCATGGAA AAGCGATGTG CACAGATATC 
CTTTTCAAGG ATGTTATTCA AGCCATCAAG GAAACTGCCT TTGTCACGTC GGAATACCCT 
GTGATTCTCT CCTTTGAAAA TCACTGCAGC AAATATCAAC AGTACAAGAT GTCCAAATAT 
TGTGAAGATC TATTTGGAGA TCTCCTGTTG AAACAAGCAC TTGAATCACA TCCACTGGAA 
CCAGGCAGAC CCTTGCCATC CCCGAATGAC CTCAAAAGAA AAATACTCAT CAAAAACAAA 
CGACTGAAAC CTGAGGTTGA GAAAAAACAG CTTGAAGCTT TGAAAAGCGT GATGGAAGCA 
GGAGAATCCG CTGCCCCAGT GAACATCCTA GAGGATGACA ATGAAGAGGA AATAGAAAGT 
GCTGAGCAAG AGGAGGAAGC TCACCCTGAA TACAAATATG GAAATGAACT TTCTGCAGAT 
GACTTGGGTC ACAAGGAAGC TATTGCCAAT AGTGTCAAGA AGGCTTCAGA TGACCTTGAA 
CATGAAAACA GCAAAAAGGG CCTGGTTACC GTAGAAGATG AGCAGGCGTG GATGGCGTCT 
TATAAATATG TGGGTGCTAC CACTAATATC CATCCATATT TGTCCACAAT GATCAACTAC 
GCACAGCCTG TAAAGTTTCA AGGTTTCCAT GTGGCTGAAG AACGCAATAT TCATTACAAC 
ATGTCTTCTT TTAATGAGTC AGTCGGTCTA GGCTACTTGA AGACACACGC CATAGAATTT 
GTGAATTATA ACAAACGGCA AATGAGTCGC ATTTACCCCA AGGGAGGGCG AGTGGACTCC 
AGTAATTACA TGCCTCAGAT TTTCTGGAAC GCTGGCTGCC AGATGGTTTC GCTGAACTAT 
CAAACCCCAG ATTTAGCGAT GCAATTGAAT CAGGGGAAAT TTGAGTATAA TGGATCCTGC 
GGATACCTTC TCAAGCCAGA TTTCATGAGA CGGCCCGATC GAACATTCGA CCCTTTCTCT 
GAAACGCCTG TTGACGGAGT TATTGCAGCC ACTTGCTCCG TGCAGGTCAT ATCAGGTCAG 
TTCTTATCAG ATAAGAAAAT TGGCACGTAT GTAGAAGTGG ACATGTATGG GCTGCCCACG 
GACACTATCC GAAAGGAGTT CCGCACTCGC ATGGTGATGA ACAATGGTCT CAATCCCGTT 
TACAATGAAG AGTCATTTGT GTTTCGGAAG GTGATCCTCC CCGACCTGGC TGTCTTGAGG 
ATCGCCGTGT ATGACGACAA CAACAAGCTG ATCGGCCAGA GGATTCTGCC CCTGGATGGC 
CTGCAAGCAG GCTATCGACA CATATCCCTC CGGAATGAGG GAAATAAACC TTTATCGCTG 
CCAACCATTT TCTGCAACAT TGTCCTAAAA ACATATGTGC CTGATGGATT CGGAGATATT 
GTGGATGCTT TATCAGATCC AAAGAAATTT CTTTCCATTA CGGAAAAGAG AGCGGATCAA 
ATGAGAGCTA TGGGCATTGA AACTAGTGAC ATCGCCGATG TGCCCAGTGA CACTTCAAAG 
AACGACAAGA AAGGGAAAGC CAACACAGCC AAAGCGAATG TGACCCCTCA GAGCAGCTCG 
GAGCTCAGGC CAACCACCAC AGCTGCCCTG GGGGCTGGCC TGGAGGCCAA GAAAGGTACC 
ACCATAAGCG TGGGCGGTAT CGAACTGATC CCTCAAGTGA GGATAGAAGA TTTAAAGCAG 
ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA CAACAGAAAG AGCTCAATTC TTTAAAGAAG 
AAACATGCAA AGGAACACAG CACCATGCAG AAGCTACACT GCACGCAAGT TGACAAGATT 
GTGGCACAGT ACGACAAGGA GAAATTGACT CATGAGAAAA TCCTCGAGAA GGCGATGAAG 
AAGAAAGGGG GAAGTAATTG TCTTGAAATG AAGAAAGAAA CAGAAATTAA AATTCAGACG 
CTGACATCAG ATCACAAATC TAAGGTCAAG GAGATTGTGG CACAGCACAC AAAGGAGTGG 
TCAGACATGA TCAACACCCA CAGTGCGGAG GAGCAGGAGG TCCGGGACCT GCACCTCAGC 
CAGCAATGCG AACTGCTCAG AAAGCTGCTG ATCAGTGCCC ACGAGCAGCA GACCCAGCAG 
CTGAAACTGT CCCATGACAG GGAAAGCAAA GAGATGCGCG CCCACCAAGC TAAGATTTCT 
ATGGAAAATA GCAAAGCCAT CAGCCAAGAT AAATCCATCA AGAATAAAGC AGAACGGGAA 
AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA 
CTCGCCATGA AGCAGTCCAA AGAAATGGAT CAGTTGAAAA AAGTCCAGCT GGAGCACCTC 
GAATTCCTAG AGAAACAGAA TGAGCAGCTT TTGAAATCCT GTCATGCAGT GTCCCAAACT 
CAAGGCGAAG GAGATGCAGC AGATGGTGAA ATTGGAAGCC GAGATGGACC GCAGACCAGC 
AACAGTTGTG TGAAACTCCA GGACACAAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012018298.1
CDS	2698..5910
Translation	MTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012162908.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162908.2

ORF Insert Sequence:

ATGACGTGCC TCAAGAAGCA CTGGATGAAA TTGGCATTTA TGACCAACAC AAATGGGAAA 
ATTCCAGTTA GGAGTATTAC TAGAACCTTT GCATCGGGGA AAACAGAAAA GGTGATCTTT 
CAAGCGCTCA AGGAGCTAGG TCTTCCCAGT GGAAAGAATG ATGAAATTGA GCCTGCCGCA 
TTTACTTATG AAAAGTTCTA TGAACTGACA CAAAAGATTT GTCCTCGGAC AGATATAGAG 
GATCTTTTCA AAAAAATCAA TGGAGACAAA ACTGATTATT TAACGGTAGA CCAATTAGTG 
AGCTTTCTAA ATGAACATCA ACGAGATCCT CGACTGAATG AAATTTTATT CCCATTTTAT 
GATGCTAAAA GGGCAATGCA GATCATTGAG ATGTATGAGC CTGATGAAGA TTTGAAGAAA 
CAAGGCCTCA TATCAAGTGA TGGGTTTTGC AGATACCTGA TGTCAGATGA AAATGCCCCC 
GTCTTCCTAG ATCGTCTAGA GCTGTACCAA GAAATGGACC ACCCTCTGGC TCACTACTTC 
ATCAGCTCTT CCCACAACAC CTATCTCACT GGCAGACAGT TTGGTGGGAA ATCTTCAGTA 
GAAATGTACA GACAGGTCCT CCTGGCTGGT TGCAGATGTG TTGAACTTGA CTGTTGGGAT 
GGAAAAGGTG AGGACCAAGA ACCGATAATA ACTCATGGAA AAGCGATGTG CACAGATATC 
CTTTTCAAGG ATGTTATTCA AGCCATCAAG GAAACTGCCT TTGTCACGTC GGAATACCCT 
GTGATTCTCT CCTTTGAAAA TCACTGCAGC AAATATCAAC AGTACAAGAT GTCCAAATAT 
TGTGAAGATC TATTTGGAGA TCTCCTGTTG AAACAAGCAC TTGAATCACA TCCACTGGAA 
CCAGGCAGAC CCTTGCCATC CCCGAATGAC CTCAAAAGAA AAATACTCAT CAAAAACAAA 
CGACTGAAAC CTGAGGTTGA GAAAAAACAG CTTGAAGCTT TGAAAAGCGT GATGGAAGCA 
GGAGAATCCG CTGCCCCAGT GAACATCCTA GAGGATGACA ATGAAGAGGA AATAGAAAGT 
GCTGAGCAAG AGGAGGAAGC TCACCCTGAA TACAAATATG GAAATGAACT TTCTGCAGAT 
GACTTGGGTC ACAAGGAAGC TATTGCCAAT AGTGTCAAGA AGGCTTCAGA TGACCTTGAA 
CATGAAAACA GCAAAAAGGG CCTGGTTACC GTAGAAGATG AGCAGGCGTG GATGGCGTCT 
TATAAATATG TGGGTGCTAC CACTAATATC CATCCATATT TGTCCACAAT GATCAACTAC 
GCACAGCCTG TAAAGTTTCA AGGTTTCCAT GTGGCTGAAG AACGCAATAT TCATTACAAC 
ATGTCTTCTT TTAATGAGTC AGTCGGTCTA GGCTACTTGA AGACACACGC CATAGAATTT 
GTGAATTATA ACAAACGGCA AATGAGTCGC ATTTACCCCA AGGGAGGGCG AGTGGACTCC 
AGTAATTACA TGCCTCAGAT TTTCTGGAAC GCTGGCTGCC AGATGGTTTC GCTGAACTAT 
CAAACCCCAG ATTTAGCGAT GCAATTGAAT CAGGGGAAAT TTGAGTATAA TGGATCCTGC 
GGATACCTTC TCAAGCCAGA TTTCATGAGA CGGCCCGATC GAACATTCGA CCCTTTCTCT 
GAAACGCCTG TTGACGGAGT TATTGCAGCC ACTTGCTCCG TGCAGGTCAT ATCAGGTCAG 
TTCTTATCAG ATAAGAAAAT TGGCACGTAT GTAGAAGTGG ACATGTATGG GCTGCCCACG 
GACACTATCC GAAAGGAGTT CCGCACTCGC ATGGTGATGA ACAATGGTCT CAATCCCGTT 
TACAATGAAG AGTCATTTGT GTTTCGGAAG GTGATCCTCC CCGACCTGGC TGTCTTGAGG 
ATCGCCGTGT ATGACGACAA CAACAAGCTG ATCGGCCAGA GGATTCTGCC CCTGGATGGC 
CTGCAAGCAG GCTATCGACA CATATCCCTC CGGAATGAGG GAAATAAACC TTTATCGCTG 
CCAACCATTT TCTGCAACAT TGTCCTAAAA ACATATGTGC CTGATGGATT CGGAGATATT 
GTGGATGCTT TATCAGATCC AAAGAAATTT CTTTCCATTA CGGAAAAGAG AGCGGATCAA 
ATGAGAGCTA TGGGCATTGA AACTAGTGAC ATCGCCGATG TGCCCAGTGA CACTTCAAAG 
AACGACAAGA AAGGGAAAGC CAACACAGCC AAAGCGAATG TGACCCCTCA GAGCAGCTCG 
GAGCTCAGGC CAACCACCAC AGCTGCCCTG GGGGCTGGCC TGGAGGCCAA GAAAGGTACC 
ACCATAAGCG TGGGCGGTAT CGAACTGATC CCTCAAGTGA GGATAGAAGA TTTAAAGCAG 
ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA CAACAGAAAG AGCTCAATTC TTTAAAGAAG 
AAACATGCAA AGGAACACAG CACCATGCAG AAGCTACACT GCACGCAAGT TGACAAGATT 
GTGGCACAGT ACGACAAGGA GAAATTGACT CATGAGAAAA TCCTCGAGAA GGCGATGAAG 
AAGAAAGGGG GAAGTAATTG TCTTGAAATG AAGAAAGAAA CAGAAATTAA AATTCAGACG 
CTGACATCAG ATCACAAATC TAAGGTCAAG GAGATTGTGG CACAGCACAC AAAGGAGTGG 
TCAGACATGA TCAACACCCA CAGTGCGGAG GAGCAGGAGG TCCGGGACCT GCACCTCAGC 
CAGCAATGCG AACTGCTCAG AAAGCTGCTG ATCAGTGCCC ACGAGCAGCA GACCCAGCAG 
CTGAAACTGT CCCATGACAG GGAAAGCAAA GAGATGCGCG CCCACCAAGC TAAGATTTCT 
ATGGAAAATA GCAAAGCCAT CAGCCAAGAT AAATCCATCA AGAATAAAGC AGAACGGGAA 
AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA 
CTCGCCATGA AGCAGTCCAA AGAAATGGAT CAGTTGAAAA AAGTCCAGCT GGAGCACCTC 
GAATTCCTAG AGAAACAGAA TGAGCAGCTT TTGAAATCCT GTCATGCAGT GTCCCAAACT 
CAAGGCGAAG GAGATGCAGC AGATGGTGAA ATTGGAAGCC GAGATGGACC GCAGACCAGC 
AACAGTTGTG TGAAACTCCA GGACACAAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_027976469.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027832270.1 Ovis aries phospholipase C beta 4 (PLCB4), mRNA.

Price & Availability↑

CloneID	OSm62516
Clone ID Related Accession (Same CDS sequence)	XM_027976469.1
Accession Version	XM_027976469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040264.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTT 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AGCCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTATCG AACTGATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC 
TTGAAGCATT TAAAGAAACA ACAGAAAGAG CTCAATTCTT TAAAGAAGAA ACATGCAAAG 
GAACACAGCA CCATGCAGAA GCTACACTGC ACGCAAGTTG ACAAGATTGT GGCACAGTAC 
GACAAGGAGA AATTGACTCA TGAGAAAATC CTCGAGAAGG CGATGAAGAA GAAAGGGGGA 
AGTAATTGTC TTGAAATGAA GAAAGAAACA GAAATTAAAA TTCAGACGCT GACATCAGAT 
CACAAATCTA AGGTCAAGGA GATTGTGGCA CAGCACACAA AGGAGTGGTC AGACATGATC 
AACACCCACA GTGCGGAGGA GCAGGAGGTC CGGGACCTGC ACCTCAGCCA GCAATGCGAA 
CTGCTCAGAA AGCTGCTGAT CAGTGCCCAC GAGCAGCAGA CCCAGCAGTT GAAACTGTCC 
CATGACAGGG AAAGCAAAGA GATGCGAGCC CACCAAGCTA AGATTTCTAT GGAAAATAGC 
AAAGCCATCA GCCAAGATAA ATCCATCAAG AATAAAGCAG AACGGGAAAG GAGAGTCAGG 
GAGTTAAATA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT CGCCATGAAG 
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTGG AGCACCTCGA ATTCCTAGAG 
AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC 
CGCAGACCAG CAACAGTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027832270.1
CDS	266..3829
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPATTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV

Target ORF information:

RefSeq Version	XM_027976469.1
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027976469.1

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GATACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGAGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACGTATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCATTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTT 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AGCCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTATCG AACTGATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC 
TTGAAGCATT TAAAGAAACA ACAGAAAGAG CTCAATTCTT TAAAGAAGAA ACATGCAAAG 
GAACACAGCA CCATGCAGAA GCTACACTGC ACGCAAGTTG ACAAGATTGT GGCACAGTAC 
GACAAGGAGA AATTGACTCA TGAGAAAATC CTCGAGAAGG CGATGAAGAA GAAAGGGGGA 
AGTAATTGTC TTGAAATGAA GAAAGAAACA GAAATTAAAA TTCAGACGCT GACATCAGAT 
CACAAATCTA AGGTCAAGGA GATTGTGGCA CAGCACACAA AGGAGTGGTC AGACATGATC 
AACACCCACA GTGCGGAGGA GCAGGAGGTC CGGGACCTGC ACCTCAGCCA GCAATGCGAA 
CTGCTCAGAA AGCTGCTGAT CAGTGCCCAC GAGCAGCAGA CCCAGCAGTT GAAACTGTCC 
CATGACAGGG AAAGCAAAGA GATGCGAGCC CACCAAGCTA AGATTTCTAT GGAAAATAGC 
AAAGCCATCA GCCAAGATAA ATCCATCAAG AATAAAGCAG AACGGGAAAG GAGAGTCAGG 
GAGTTAAATA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT CGCCATGAAG 
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTGG AGCACCTCGA ATTCCTAGAG 
AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC 
CGCAGACCAG CAACAGTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_015099525.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014955011.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA.

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CloneID	OSm27692
Clone ID Related Accession (Same CDS sequence)	XM_015099525.1 , XM_012188100.2 , XM_015099526.1 , XM_012188102.2 , XM_012188101.2
Accession Version	XM_015099525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014955011.1
CDS	1301..4960
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_015099525.1
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015099525.1

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188100.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043490.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm27692
Clone ID Related Accession (Same CDS sequence)	XM_015099525.1 , XM_012188100.2 , XM_015099526.1 , XM_012188102.2 , XM_012188101.2
Accession Version	XM_012188100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043490.1
CDS	452..4111
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188100.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188100.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_015099526.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014955012.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSm27692
Clone ID Related Accession (Same CDS sequence)	XM_015099525.1 , XM_012188100.2 , XM_015099526.1 , XM_012188102.2 , XM_012188101.2
Accession Version	XM_015099526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014955012.1
CDS	1464..5123
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_015099526.1
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015099526.1

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188102.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043492.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA.

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CloneID	OSm27692
Clone ID Related Accession (Same CDS sequence)	XM_015099525.1 , XM_012188100.2 , XM_015099526.1 , XM_012188102.2 , XM_012188101.2
Accession Version	XM_012188102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188102.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043492.1
CDS	3147..6806
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188102.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188102.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188101.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043491.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OSm27692
Clone ID Related Accession (Same CDS sequence)	XM_015099525.1 , XM_012188100.2 , XM_015099526.1 , XM_012188102.2 , XM_012188101.2
Accession Version	XM_012188101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043491.1
CDS	615..4274
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188101.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188101.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGCTTTTG 
AAATCCTGTC ATGCAGTGTC CCAAACTCAA GGCGAAGGAG ATGCAGCAGA TGGTGAAATT 
GGAAGCCGAG ATGGACCGCA GACCAGCAAC AGTTGTGTGA AACTCCAGGA CACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188107.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043497.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OSm27693
Clone ID Related Accession (Same CDS sequence)	XM_012188107.2 , XM_012188105.2 , XM_012188104.2 , XM_012188106.2
Accession Version	XM_012188107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GGTACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCGTTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CGATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGAGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043497.1
CDS	393..4034
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188107.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188107.2

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GGTACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCGTTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CGATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGAGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188105.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043495.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X9, mRNA.

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CloneID	OSm27693
Clone ID Related Accession (Same CDS sequence)	XM_012188107.2 , XM_012188105.2 , XM_012188104.2 , XM_012188106.2
Accession Version	XM_012188105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GGTACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCGTTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CGATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGAGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043495.1
CDS	615..4256
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188105.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188105.2

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GGTACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCGTTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CGATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGAGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188104.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043494.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OSm27693
Clone ID Related Accession (Same CDS sequence)	XM_012188107.2 , XM_012188105.2 , XM_012188104.2 , XM_012188106.2
Accession Version	XM_012188104.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188104.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GGTACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCGTTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CGATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGAGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043494.1
CDS	452..4093
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188104.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188104.2

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GGTACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCGTTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CGATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGAGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188106.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043496.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSm27693
Clone ID Related Accession (Same CDS sequence)	XM_012188107.2 , XM_012188105.2 , XM_012188104.2 , XM_012188106.2
Accession Version	XM_012188106.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GGTACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCGTTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CGATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGAGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043496.1
CDS	3147..6788
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188106.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188106.2

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA 
GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAGGTGCTA 
GAATGTTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC 
TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATCCAGAA 
ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC 
GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCGCTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG 
CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC 
CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAT 
TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCGATAATAA CTCATGGAAA AGCGATGTGC 
ACAGATATCC TTTTCAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACGTCG 
GAATACCCTG TGATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGACC CTTGCCATCC CCGAATGACC TCAAAAGAAA AATACTCATC 
AAAAACAAAC GACTGAAACC TGAGGTTGAG AAAAAACAGC TTGAAGCTTT GAAAAGCGTG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCCTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAGGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG 
ATGGCGTCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG 
ATCAACTACG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT 
CATTACAACA TGTCTTCTTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC 
ATAGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGGCGA 
GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGGAAATT TGAGTATAAT 
GGATCCTGCG GGTACCTTCT CAAGCCAGAT TTCATGAGAC GGCCCGATCG AACATTCGAC 
CCTTTCTCTG AAACGCCTGT TGACGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA CATGTATGGG 
CTGCCCACGG ACACTATCCG AAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGTCTC 
AATCCCGTTT ACAATGAAGA GTCGTTTGTG TTTCGGAAGG TGATCCTCCC TGACCTGGCT 
GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC 
CTGGATGGCC TGCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT 
TTATCGCTGC CAACCATTTT CTGCAACATT GTCCTAAAAA CATATGTGCC TGATGGATTC 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC 
ACGTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG 
AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG 
AAAGGTACCA CGATAAGCGT GGGCGGTATC GAACTGATCC CTCAAGTGAG GATAGAAGAT 
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AACAGAAAGA GCTCAATTCT 
TTAAAGAAGA AACATGCAAA GGAACACAGC ACCATGCAGA AGCTACACTG CACGCAAGTT 
GACAAGATTG TGGCACAGTA CGACAAGGAG AAATTGACTC ATGAGAAAAT CCTCGAGAAG 
GCGATGAAGA AGAAAGGGGG AAGTAATTGT CTTGAAATGA AGAAAGAAAC AGAAATTAAA 
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAGG AGATTGTGGC ACAGCACACA 
AAGGAGTGGT CAGACATGAT CAACACCCAC AGTGCGGAGG AGCAGGAGGT CCGGGACCTG 
CACCTCAGCC AGCAATGCGA ACTGCTCAGA AAGCTGCTGA TCAGTGCCCA CGAGCAGCAG 
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAAG AGATGCGAGC CCACCAAGCT 
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCCATCAA GAATAAAGCA 
GAACGGGAAA GGAGAGTCAG GGAGTTAAAT AGCAGCAACA CTAAAAAGTT TCTGGAAGAA 
AGAAAGAGAC TCGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTG 
GAGCACCTCG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG 
TCCCAAACTC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG 
CAGACCAGCA ACAGTTGTGT GAAACTCCAG GACACAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188108.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043498.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X11, mRNA.

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CloneID	OSm27694
Clone ID Related Accession (Same CDS sequence)	XM_012188108.2
Accession Version	XM_012188108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTATCGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA 
AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAATTCTTTA 
AAGAAGAAAC ATGCAAAGGA ACACAGCACC ATGCAGAAGC TACACTGCAC GCAAGTTGAC 
AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCATG AGAAAATCCT CGAGAAGGCG 
ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT 
CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG 
GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGGTCCG GGACCTGCAC 
CTCAGCCAGC AATGCGAACT GCTCAGAAAG CTGCTGATCA GTGCCCACGA GCAGCAGACC 
CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCCCA CCAAGCTAAG 
ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA 
CGGGAAAGGA GAGTCAGGGA GTTAAATAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA 
AAGAGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTGGAG 
CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA TGCAGTGTCC 
CAAACTCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG 
ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043498.1
CDS	452..4090
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188108.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188108.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTATCGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA 
AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAATTCTTTA 
AAGAAGAAAC ATGCAAAGGA ACACAGCACC ATGCAGAAGC TACACTGCAC GCAAGTTGAC 
AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCATG AGAAAATCCT CGAGAAGGCG 
ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT 
CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG 
GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGGTCCG GGACCTGCAC 
CTCAGCCAGC AATGCGAACT GCTCAGAAAG CTGCTGATCA GTGCCCACGA GCAGCAGACC 
CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCCCA CCAAGCTAAG 
ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA 
CGGGAAAGGA GAGTCAGGGA GTTAAATAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA 
AAGAGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTGGAG 
CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA TGCAGTGTCC 
CAAACTCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG 
ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188109.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043499.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OSm27695
Clone ID Related Accession (Same CDS sequence)	XM_012188109.2
Accession Version	XM_012188109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188109.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGGTACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACATA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGTC TCAATCCCGT TTACAATGAA 
GAGTCGTTTG TGTTTCGGAA GGTGATCCTC CCTGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACGTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAC CACGATAAGC 
GTGGGCGGTA TCGAACTGAT CCCTCAAGTG AGGATAGAAG ATTTAAAGCA GATGAAGGCT 
TACTTGAAGC ATTTAAAGAA ACAACAGAAA GAGCTCAATT CTTTAAAGAA GAAACATGCA 
AAGGAACACA GCACCATGCA GAAGCTACAC TGCACGCAAG TTGACAAGAT TGTGGCACAG 
TACGACAAGG AGAAATTGAC TCATGAGAAA ATCCTCGAGA AGGCGATGAA GAAGAAAGGG 
GGAAGTAATT GTCTTGAAAT GAAGAAAGAA ACAGAAATTA AAATTCAGAC GCTGACATCA 
GATCACAAAT CTAAGGTCAA GGAGATTGTG GCACAGCACA CAAAGGAGTG GTCAGACATG 
ATCAACACCC ACAGTGCGGA GGAGCAGGAG GTCCGGGACC TGCACCTCAG CCAGCAATGC 
GAACTGCTCA GAAAGCTGCT GATCAGTGCC CACGAGCAGC AGACCCAGCA GCTGAAACTG 
TCCCATGACA GGGAAAGCAA AGAGATGCGA GCCCACCAAG CTAAGATTTC TATGGAAAAT 
AGCAAAGCCA TCAGCCAAGA TAAATCCATC AAGAATAAAG CAGAACGGGA AAGGAGAGTC 
AGGGAGTTAA ATAGCAGCAA CACTAAAAAG TTTCTGGAAG AAAGAAAGAG ACTCGCCATG 
AAGCAGTCCA AAGAAATGGA TCAGTTGAAA AAAGTCCAGC TGGAGCACCT CGAATTCCTA 
GAGAAACAGA ATGAGCAGCT TTTGAAATCC TGTCATGCAG TGTCCCAAAC TCAAGGCGAA 
GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC CGCAGACCAG CAACAGTTGT 
GTGAAACTCC AGGACACAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043499.1
CDS	452..4075
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188109.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188109.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGGTACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACATA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGTC TCAATCCCGT TTACAATGAA 
GAGTCGTTTG TGTTTCGGAA GGTGATCCTC CCTGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACGTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAC CACGATAAGC 
GTGGGCGGTA TCGAACTGAT CCCTCAAGTG AGGATAGAAG ATTTAAAGCA GATGAAGGCT 
TACTTGAAGC ATTTAAAGAA ACAACAGAAA GAGCTCAATT CTTTAAAGAA GAAACATGCA 
AAGGAACACA GCACCATGCA GAAGCTACAC TGCACGCAAG TTGACAAGAT TGTGGCACAG 
TACGACAAGG AGAAATTGAC TCATGAGAAA ATCCTCGAGA AGGCGATGAA GAAGAAAGGG 
GGAAGTAATT GTCTTGAAAT GAAGAAAGAA ACAGAAATTA AAATTCAGAC GCTGACATCA 
GATCACAAAT CTAAGGTCAA GGAGATTGTG GCACAGCACA CAAAGGAGTG GTCAGACATG 
ATCAACACCC ACAGTGCGGA GGAGCAGGAG GTCCGGGACC TGCACCTCAG CCAGCAATGC 
GAACTGCTCA GAAAGCTGCT GATCAGTGCC CACGAGCAGC AGACCCAGCA GCTGAAACTG 
TCCCATGACA GGGAAAGCAA AGAGATGCGA GCCCACCAAG CTAAGATTTC TATGGAAAAT 
AGCAAAGCCA TCAGCCAAGA TAAATCCATC AAGAATAAAG CAGAACGGGA AAGGAGAGTC 
AGGGAGTTAA ATAGCAGCAA CACTAAAAAG TTTCTGGAAG AAAGAAAGAG ACTCGCCATG 
AAGCAGTCCA AAGAAATGGA TCAGTTGAAA AAAGTCCAGC TGGAGCACCT CGAATTCCTA 
GAGAAACAGA ATGAGCAGCT TTTGAAATCC TGTCATGCAG TGTCCCAAAC TCAAGGCGAA 
GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC CGCAGACCAG CAACAGTTGT 
GTGAAACTCC AGGACACAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_004014147.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004014196.2 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X13, mRNA.

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CloneID	OSm27696
Clone ID Related Accession (Same CDS sequence)	XM_004014147.3
Accession Version	XM_004014147.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004014147.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGGTACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACATA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGTC TCAATCCCGT TTACAATGAA 
GAGTCGTTTG TGTTTCGGAA GGTGATCCTC CCTGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACGTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT CGAACTGATC 
CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA 
CAACAGAAAG AGCTCAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG CACCATGCAG 
AAGCTACACT GCACGCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT 
CATGAGAAAA TCCTCGAGAA GGCGATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG 
AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG 
GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG 
GAGCAGGAGG TCCGGGACCT GCACCTCAGC CAGCAATGCG AACTGCTCAG AAAGCTGCTG 
ATCAGTGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA 
GAGATGCGAG CCCACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT 
AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC 
ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT 
CAGTTGAAAA AAGTCCAGCT GGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGCTT 
TTGAAATCCT GTCATGCAGT GTCCCAAACT CAAGGCGAAG GAGATGCAGC AGATGGTGAA 
ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG TGAAACTCCA GGACACAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004014196.2
CDS	451..4053
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_004014147.3
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004014147.3

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGGTACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACATA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGTC TCAATCCCGT TTACAATGAA 
GAGTCGTTTG TGTTTCGGAA GGTGATCCTC CCTGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACGTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT CGAACTGATC 
CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA 
CAACAGAAAG AGCTCAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG CACCATGCAG 
AAGCTACACT GCACGCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT 
CATGAGAAAA TCCTCGAGAA GGCGATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG 
AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG 
GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG 
GAGCAGGAGG TCCGGGACCT GCACCTCAGC CAGCAATGCG AACTGCTCAG AAAGCTGCTG 
ATCAGTGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA 
GAGATGCGAG CCCACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT 
AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC 
ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT 
CAGTTGAAAA AAGTCCAGCT GGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGCTT 
TTGAAATCCT GTCATGCAGT GTCCCAAACT CAAGGCGAAG GAGATGCAGC AGATGGTGAA 
ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG TGAAACTCCA GGACACAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188110.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043500.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X14, mRNA.

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CloneID	OSm27697
Clone ID Related Accession (Same CDS sequence)	XM_012188110.2
Accession Version	XM_012188110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGGCGAAG 
GAGATGCAGC AGATGGTGAA ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043500.1
CDS	453..4055
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV

Target ORF information:

RefSeq Version	XM_012188110.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188110.2

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTACCACG ATAAGCGTGG GCGGTATCGA ACTGATCCCT 
CAAGTGAGGA TAGAAGATTT AAAGCAGATG AAGGCTTACT TGAAGCATTT AAAGAAACAA 
CAGAAAGAGC TCAATTCTTT AAAGAAGAAA CATGCAAAGG AACACAGCAC CATGCAGAAG 
CTACACTGCA CGCAAGTTGA CAAGATTGTG GCACAGTACG ACAAGGAGAA ATTGACTCAT 
GAGAAAATCC TCGAGAAGGC GATGAAGAAG AAAGGGGGAA GTAATTGTCT TGAAATGAAG 
AAAGAAACAG AAATTAAAAT TCAGACGCTG ACATCAGATC ACAAATCTAA GGTCAAGGAG 
ATTGTGGCAC AGCACACAAA GGAGTGGTCA GACATGATCA ACACCCACAG TGCGGAGGAG 
CAGGAGGTCC GGGACCTGCA CCTCAGCCAG CAATGCGAAC TGCTCAGAAA GCTGCTGATC 
AGTGCCCACG AGCAGCAGAC CCAGCAGCTG AAACTGTCCC ATGACAGGGA AAGCAAAGAG 
ATGCGAGCCC ACCAAGCTAA GATTTCTATG GAAAATAGCA AAGCCATCAG CCAAGATAAA 
TCCATCAAGA ATAAAGCAGA ACGGGAAAGG AGAGTCAGGG AGTTAAATAG CAGCAACACT 
AAAAAGTTTC TGGAAGAAAG AAAGAGACTC GCCATGAAGC AGTCCAAAGA AATGGATCAG 
TTGAAAAAAG TCCAGCTGGA GCACCTCGAA TTCCTAGAGA AACAGAATGA GCAGGCGAAG 
GAGATGCAGC AGATGGTGAA ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_004014148.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004014197.2 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OSm27698
Clone ID Related Accession (Same CDS sequence)	XM_004014148.3
Accession Version	XM_004014148.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004014148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTATCGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA 
AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAATTCTTTA 
AAGAAGAAAC ATGCAAAGGA ACACAGCACC ATGCAGAAGC TACACTGCAC GCAAGTTGAC 
AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCATG AGAAAATCCT CGAGAAGGCG 
ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT 
CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG 
GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGGTCCG GGACCTGCAC 
CTCAGCCAGC AATGCGAACT GCTCAGAAAG CTGCTGATCA GTGCCCACGA GCAGCAGACC 
CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCCCA CCAAGCTAAG 
ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA 
CGGGAAAGGA GAGTCAGGGA GTTAAATAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA 
AAGAGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTGGAG 
CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGGCGAAGG AGATGCAGCA GATGGTGAAA 
TTGGAAGCCG AGATGGACCG CAGACCAGCA ACAGTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004014197.2
CDS	453..4034
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV

Target ORF information:

RefSeq Version	XM_004014148.3
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004014148.3

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA 
GAAGATGAGC AGGCGTGGAT GGCGTCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT 
CCGTATTTGT CCACAATGAT CAACTACGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG 
GCTGAAGAAC GCAATATTCA TTACAACATG TCTTCTTTTA ATGAGTCAGT CGGTCTAGGC 
TACTTGAAGA CACACGCCAT AGAATTTGTG AATTATAACA AACGGCAAAT GAGTCGCATT 
TACCCCAAGG GAGGGCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT 
GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA ATTGAATCAG 
GGGAAATTTG AGTATAATGG ATCCTGCGGG TACCTTCTCA AGCCAGATTT CATGAGACGG 
CCCGATCGAA CATTCGACCC TTTCTCTGAA ACGCCTGTTG ACGGGGTTAT TGCAGCCACT 
TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA 
GAAGTGGACA TGTATGGGCT GCCCACGGAC ACTATCCGAA AGGAGTTCCG CACTCGCATG 
GTGATGAACA ACGGTCTCAA TCCCGTTTAC AATGAAGAGT CGTTTGTGTT TCGGAAGGTG 
ATCCTCCCTG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC 
GGCCAGAGGA TTCTGCCCCT GGATGGCCTG CAAGCAGGCT ATCGACACAT ATCCCTCCGG 
AATGAGGGAA ATAAACCTTT ATCGCTGCCA ACCATTTTCT GCAACATTGT CCTAAAAACA 
TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT 
TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC 
GCCGATGTGC CCAGTGACAC GTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA 
GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG 
GCTGGCCTGG AGGCCAAGAA AGGTATCGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA 
AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAATTCTTTA 
AAGAAGAAAC ATGCAAAGGA ACACAGCACC ATGCAGAAGC TACACTGCAC GCAAGTTGAC 
AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCATG AGAAAATCCT CGAGAAGGCG 
ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT 
CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG 
GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGGTCCG GGACCTGCAC 
CTCAGCCAGC AATGCGAACT GCTCAGAAAG CTGCTGATCA GTGCCCACGA GCAGCAGACC 
CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCCCA CCAAGCTAAG 
ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA 
CGGGAAAGGA GAGTCAGGGA GTTAAATAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA 
AAGAGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTGGAG 
CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGGCGAAGG AGATGCAGCA GATGGTGAAA 
TTGGAAGCCG AGATGGACCG CAGACCAGCA ACAGTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_004014146.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004014195.2 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X16, mRNA.

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CloneID	OSm27699
Clone ID Related Accession (Same CDS sequence)	XM_004014146.3
Accession Version	XM_004014146.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004014146.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGGTACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACATA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGTC TCAATCCCGT TTACAATGAA 
GAGTCGTTTG TGTTTCGGAA GGTGATCCTC CCTGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACGTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT CGAACTGATC 
CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA 
CAACAGAAAG AGCTCAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG CACCATGCAG 
AAGCTACACT GCACGCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT 
CATGAGAAAA TCCTCGAGAA GGCGATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG 
AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG 
GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG 
GAGCAGGAGG TCCGGGACCT GCACCTCAGC CAGCAATGCG AACTGCTCAG AAAGCTGCTG 
ATCAGTGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA 
GAGATGCGAG CCCACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT 
AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC 
ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT 
CAGTTGAAAA AAGTCCAGCT GGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGGCG 
AAGGAGATGC AGCAGATGGT GAAATTGGAA GCCGAGATGG ACCGCAGACC AGCAACAGTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004014195.2
CDS	452..3997
Translation	MVLTLIMAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEALGKSENDLEGRIVCVCSGADLVNISFTYMVAENPEITKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV

Target ORF information:

RefSeq Version	XM_004014146.3
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004014146.3

ORF Insert Sequence:

ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA 
CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC 
AAGGAAGGCC AGGTGCTAGA ATGTTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCA 
AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA 
GGGCGGATAG TTTGTGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACATACATG 
GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC 
AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG 
GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA 
TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCGCTCAAGG AGCTAGGTCT TCCCAGTGGA 
AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA 
AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT 
GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA 
CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG 
TATGAGCCTG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA 
TACCTGATGT CAGATGAAAA TGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA 
ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC 
AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC 
AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAAGAACC GATAATAACT 
CATGGAAAAG CGATGTGCAC AGATATCCTT TTCAAGGATG TTATTCAAGC CATCAAGGAA 
ACTGCCTTTG TCACGTCGGA ATACCCTGTG ATTCTCTCCT TTGAAAATCA CTGCAGCAAA 
TATCAACAGT ACAAGATGTC CAAATATTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA 
CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC GAATGACCTC 
AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCTG AGGTTGAGAA AAAACAGCTT 
GAAGCTTTGA AAAGCGTGAT GGAAGCAGGA GAATCCGCTG CCCCAGTGAA CATCCTAGAG 
GATGACAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCTCA CCCTGAATAC 
AAATATGGAA ATGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT 
GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCGTC TTATAAATAT 
GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA CGCACAGCCT 
GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTACAA CATGTCTTCT 
TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATAGAATT TGTGAATTAT 
AACAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGGC GAGTGGACTC CAGTAATTAC 
ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA 
GATTTAGCGA TGCAATTGAA TCAGGGGAAA TTTGAGTATA ATGGATCCTG CGGGTACCTT 
CTCAAGCCAG ATTTCATGAG ACGGCCCGAT CGAACATTCG ACCCTTTCTC TGAAACGCCT 
GTTGACGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA 
GATAAGAAAA TTGGCACATA TGTAGAAGTG GACATGTATG GGCTGCCCAC GGACACTATC 
CGAAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGTC TCAATCCCGT TTACAATGAA 
GAGTCGTTTG TGTTTCGGAA GGTGATCCTC CCTGACCTGG CTGTCTTGAG GATCGCCGTG 
TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTGCAAGCA 
GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCGCT GCCAACCATT 
TTCTGCAACA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT 
TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT 
ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACGTCAAA GAACGACAAG 
AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG 
CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT CGAACTGATC 
CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA 
CAACAGAAAG AGCTCAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG CACCATGCAG 
AAGCTACACT GCACGCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT 
CATGAGAAAA TCCTCGAGAA GGCGATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG 
AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG 
GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG 
GAGCAGGAGG TCCGGGACCT GCACCTCAGC CAGCAATGCG AACTGCTCAG AAAGCTGCTG 
ATCAGTGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA 
GAGATGCGAG CCCACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT 
AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC 
ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT 
CAGTTGAAAA AAGTCCAGCT GGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGGCG 
AAGGAGATGC AGCAGATGGT GAAATTGGAA GCCGAGATGG ACCGCAGACC AGCAACAGTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_012188111.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012043501.1 Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA.

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CloneID	OSm27700
Clone ID Related Accession (Same CDS sequence)	XM_012188111.2
Accession Version	XM_012188111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012188111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTGCC TCAAGAAGCA CTGGATGAAA TTGGCATTTA TGACCAACAC AAATGGGAAA 
ATTCCAGTTA GGAGTATTAC TAGAACCTTT GCATCGGGGA AAACAGAAAA GGTGATCTTT 
CAAGCGCTCA AGGAGCTAGG TCTTCCCAGT GGAAAGAATG ATGAAATTGA GCCTGCCGCA 
TTTACTTATG AAAAGTTCTA TGAACTGACA CAAAAGATTT GTCCTCGGAC AGATATAGAG 
GATCTTTTCA AAAAAATCAA TGGAGACAAA ACTGATTATT TAACGGTAGA CCAATTAGTG 
AGCTTTCTAA ATGAACATCA ACGAGATCCT CGACTGAATG AAATTTTATT CCCATTTTAT 
GATGCTAAAA GGGCAATGCA GATCATTGAG ATGTATGAGC CTGATGAAGA TTTGAAGAAA 
CAAGGCCTCA TATCAAGTGA TGGGTTTTGC AGATACCTGA TGTCAGATGA AAATGCCCCC 
GTCTTCCTAG ATCGTCTAGA GCTGTACCAA GAAATGGACC ACCCTCTGGC TCACTACTTC 
ATCAGCTCTT CCCACAACAC CTATCTCACT GGCAGACAGT TTGGTGGGAA ATCTTCAGTA 
GAAATGTACA GACAGGTCCT CCTGGCTGGT TGCAGATGTG TTGAACTTGA CTGTTGGGAT 
GGAAAAGGTG AGGACCAAGA ACCGATAATA ACTCATGGAA AAGCGATGTG CACAGATATC 
CTTTTCAAGG ATGTTATTCA AGCCATCAAG GAAACTGCCT TTGTCACGTC GGAATACCCT 
GTGATTCTCT CCTTTGAAAA TCACTGCAGC AAATATCAAC AGTACAAGAT GTCCAAATAT 
TGTGAAGATC TATTTGGAGA TCTCCTGTTG AAACAAGCAC TTGAATCACA TCCACTGGAA 
CCAGGCAGAC CCTTGCCATC CCCGAATGAC CTCAAAAGAA AAATACTCAT CAAAAACAAA 
CGACTGAAAC CTGAGGTTGA GAAAAAACAG CTTGAAGCTT TGAAAAGCGT GATGGAAGCA 
GGAGAATCCG CTGCCCCAGT GAACATCCTA GAGGATGACA ATGAAGAGGA AATAGAAAGT 
GCTGAGCAAG AGGAGGAAGC TCACCCTGAA TACAAATATG GAAATGAACT TTCTGCAGAT 
GACTTGGGTC ACAAGGAAGC TATTGCCAAT AGTGTCAAGA AGGCTTCAGA TGACCTTGAA 
CATGAAAACA GCAAAAAGGG CCTGGTTACC GTAGAAGATG AGCAGGCGTG GATGGCGTCT 
TATAAATATG TGGGTGCTAC CACTAATATC CATCCGTATT TGTCCACAAT GATCAACTAC 
GCACAGCCTG TAAAGTTTCA AGGTTTCCAT GTGGCTGAAG AACGCAATAT TCATTACAAC 
ATGTCTTCTT TTAATGAGTC AGTCGGTCTA GGCTACTTGA AGACACACGC CATAGAATTT 
GTGAATTATA ACAAACGGCA AATGAGTCGC ATTTACCCCA AGGGAGGGCG AGTGGACTCC 
AGTAATTACA TGCCTCAGAT TTTCTGGAAC GCTGGCTGCC AGATGGTTTC GCTGAACTAT 
CAAACCCCAG ATTTAGCGAT GCAATTGAAT CAGGGGAAAT TTGAGTATAA TGGATCCTGC 
GGGTACCTTC TCAAGCCAGA TTTCATGAGA CGGCCCGATC GAACATTCGA CCCTTTCTCT 
GAAACGCCTG TTGACGGGGT TATTGCAGCC ACTTGCTCCG TGCAGGTCAT ATCAGGTCAG 
TTCTTATCAG ATAAGAAAAT TGGCACATAT GTAGAAGTGG ACATGTATGG GCTGCCCACG 
GACACTATCC GAAAGGAGTT CCGCACTCGC ATGGTGATGA ACAACGGTCT CAATCCCGTT 
TACAATGAAG AGTCGTTTGT GTTTCGGAAG GTGATCCTCC CTGACCTGGC TGTCTTGAGG 
ATCGCCGTGT ATGACGACAA CAACAAGCTG ATCGGCCAGA GGATTCTGCC CCTGGATGGC 
CTGCAAGCAG GCTATCGACA CATATCCCTC CGGAATGAGG GAAATAAACC TTTATCGCTG 
CCAACCATTT TCTGCAACAT TGTCCTAAAA ACATATGTGC CTGATGGATT CGGAGATATT 
GTGGATGCTT TATCAGATCC AAAGAAATTT CTTTCCATTA CGGAAAAGAG AGCGGATCAA 
ATGAGAGCTA TGGGCATTGA AACTAGTGAC ATCGCCGATG TGCCCAGTGA CACGTCAAAG 
AACGACAAGA AAGGGAAAGC CAACACAGCC AAAGCGAATG TGACCCCTCA GAGCAGCTCG 
GAGCTCAGGC CAACCACCAC AGCTGCCCTG GGGGCTGGCC TGGAGGCCAA GAAAGGTACC 
ACGATAAGCG TGGGCGGTAT CGAACTGATC CCTCAAGTGA GGATAGAAGA TTTAAAGCAG 
ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA CAACAGAAAG AGCTCAATTC TTTAAAGAAG 
AAACATGCAA AGGAACACAG CACCATGCAG AAGCTACACT GCACGCAAGT TGACAAGATT 
GTGGCACAGT ACGACAAGGA GAAATTGACT CATGAGAAAA TCCTCGAGAA GGCGATGAAG 
AAGAAAGGGG GAAGTAATTG TCTTGAAATG AAGAAAGAAA CAGAAATTAA AATTCAGACG 
CTGACATCAG ATCACAAATC TAAGGTCAAG GAGATTGTGG CACAGCACAC AAAGGAGTGG 
TCAGACATGA TCAACACCCA CAGTGCGGAG GAGCAGGAGG TCCGGGACCT GCACCTCAGC 
CAGCAATGCG AACTGCTCAG AAAGCTGCTG ATCAGTGCCC ACGAGCAGCA GACCCAGCAG 
CTGAAACTGT CCCATGACAG GGAAAGCAAA GAGATGCGAG CCCACCAAGC TAAGATTTCT 
ATGGAAAATA GCAAAGCCAT CAGCCAAGAT AAATCCATCA AGAATAAAGC AGAACGGGAA 
AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA 
CTCGCCATGA AGCAGTCCAA AGAAATGGAT CAGTTGAAAA AAGTCCAGCT GGAGCACCTC 
GAATTCCTAG AGAAACAGAA TGAGCAGCTT TTGAAATCCT GTCATGCAGT GTCCCAAACT 
CAAGGCGAAG GAGATGCAGC AGATGGTGAA ATTGGAAGCC GAGATGGACC GCAGACCAGC 
AACAGTTGTG TGAAACTCCA GGACACAAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012043501.1
CDS	2698..5910
Translation	MTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKQGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSVMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENSKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGTTISVGGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKLTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEVRDLHLSQQCELLRKLLISAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_012188111.2
Organism	Ovis aries(sheep)
Definition	Ovis aries phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012188111.2

ORF Insert Sequence:

ATGACGTGCC TCAAGAAGCA CTGGATGAAA TTGGCATTTA TGACCAACAC AAATGGGAAA 
ATTCCAGTTA GGAGTATTAC TAGAACCTTT GCATCGGGGA AAACAGAAAA GGTGATCTTT 
CAAGCGCTCA AGGAGCTAGG TCTTCCCAGT GGAAAGAATG ATGAAATTGA GCCTGCCGCA 
TTTACTTATG AAAAGTTCTA TGAACTGACA CAAAAGATTT GTCCTCGGAC AGATATAGAG 
GATCTTTTCA AAAAAATCAA TGGAGACAAA ACTGATTATT TAACGGTAGA CCAATTAGTG 
AGCTTTCTAA ATGAACATCA ACGAGATCCT CGACTGAATG AAATTTTATT CCCATTTTAT 
GATGCTAAAA GGGCAATGCA GATCATTGAG ATGTATGAGC CTGATGAAGA TTTGAAGAAA 
CAAGGCCTCA TATCAAGTGA TGGGTTTTGC AGATACCTGA TGTCAGATGA AAATGCCCCC 
GTCTTCCTAG ATCGTCTAGA GCTGTACCAA GAAATGGACC ACCCTCTGGC TCACTACTTC 
ATCAGCTCTT CCCACAACAC CTATCTCACT GGCAGACAGT TTGGTGGGAA ATCTTCAGTA 
GAAATGTACA GACAGGTCCT CCTGGCTGGT TGCAGATGTG TTGAACTTGA CTGTTGGGAT 
GGAAAAGGTG AGGACCAAGA ACCGATAATA ACTCATGGAA AAGCGATGTG CACAGATATC 
CTTTTCAAGG ATGTTATTCA AGCCATCAAG GAAACTGCCT TTGTCACGTC GGAATACCCT 
GTGATTCTCT CCTTTGAAAA TCACTGCAGC AAATATCAAC AGTACAAGAT GTCCAAATAT 
TGTGAAGATC TATTTGGAGA TCTCCTGTTG AAACAAGCAC TTGAATCACA TCCACTGGAA 
CCAGGCAGAC CCTTGCCATC CCCGAATGAC CTCAAAAGAA AAATACTCAT CAAAAACAAA 
CGACTGAAAC CTGAGGTTGA GAAAAAACAG CTTGAAGCTT TGAAAAGCGT GATGGAAGCA 
GGAGAATCCG CTGCCCCAGT GAACATCCTA GAGGATGACA ATGAAGAGGA AATAGAAAGT 
GCTGAGCAAG AGGAGGAAGC TCACCCTGAA TACAAATATG GAAATGAACT TTCTGCAGAT 
GACTTGGGTC ACAAGGAAGC TATTGCCAAT AGTGTCAAGA AGGCTTCAGA TGACCTTGAA 
CATGAAAACA GCAAAAAGGG CCTGGTTACC GTAGAAGATG AGCAGGCGTG GATGGCGTCT 
TATAAATATG TGGGTGCTAC CACTAATATC CATCCGTATT TGTCCACAAT GATCAACTAC 
GCACAGCCTG TAAAGTTTCA AGGTTTCCAT GTGGCTGAAG AACGCAATAT TCATTACAAC 
ATGTCTTCTT TTAATGAGTC AGTCGGTCTA GGCTACTTGA AGACACACGC CATAGAATTT 
GTGAATTATA ACAAACGGCA AATGAGTCGC ATTTACCCCA AGGGAGGGCG AGTGGACTCC 
AGTAATTACA TGCCTCAGAT TTTCTGGAAC GCTGGCTGCC AGATGGTTTC GCTGAACTAT 
CAAACCCCAG ATTTAGCGAT GCAATTGAAT CAGGGGAAAT TTGAGTATAA TGGATCCTGC 
GGGTACCTTC TCAAGCCAGA TTTCATGAGA CGGCCCGATC GAACATTCGA CCCTTTCTCT 
GAAACGCCTG TTGACGGGGT TATTGCAGCC ACTTGCTCCG TGCAGGTCAT ATCAGGTCAG 
TTCTTATCAG ATAAGAAAAT TGGCACATAT GTAGAAGTGG ACATGTATGG GCTGCCCACG 
GACACTATCC GAAAGGAGTT CCGCACTCGC ATGGTGATGA ACAACGGTCT CAATCCCGTT 
TACAATGAAG AGTCGTTTGT GTTTCGGAAG GTGATCCTCC CTGACCTGGC TGTCTTGAGG 
ATCGCCGTGT ATGACGACAA CAACAAGCTG ATCGGCCAGA GGATTCTGCC CCTGGATGGC 
CTGCAAGCAG GCTATCGACA CATATCCCTC CGGAATGAGG GAAATAAACC TTTATCGCTG 
CCAACCATTT TCTGCAACAT TGTCCTAAAA ACATATGTGC CTGATGGATT CGGAGATATT 
GTGGATGCTT TATCAGATCC AAAGAAATTT CTTTCCATTA CGGAAAAGAG AGCGGATCAA 
ATGAGAGCTA TGGGCATTGA AACTAGTGAC ATCGCCGATG TGCCCAGTGA CACGTCAAAG 
AACGACAAGA AAGGGAAAGC CAACACAGCC AAAGCGAATG TGACCCCTCA GAGCAGCTCG 
GAGCTCAGGC CAACCACCAC AGCTGCCCTG GGGGCTGGCC TGGAGGCCAA GAAAGGTACC 
ACGATAAGCG TGGGCGGTAT CGAACTGATC CCTCAAGTGA GGATAGAAGA TTTAAAGCAG 
ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA CAACAGAAAG AGCTCAATTC TTTAAAGAAG 
AAACATGCAA AGGAACACAG CACCATGCAG AAGCTACACT GCACGCAAGT TGACAAGATT 
GTGGCACAGT ACGACAAGGA GAAATTGACT CATGAGAAAA TCCTCGAGAA GGCGATGAAG 
AAGAAAGGGG GAAGTAATTG TCTTGAAATG AAGAAAGAAA CAGAAATTAA AATTCAGACG 
CTGACATCAG ATCACAAATC TAAGGTCAAG GAGATTGTGG CACAGCACAC AAAGGAGTGG 
TCAGACATGA TCAACACCCA CAGTGCGGAG GAGCAGGAGG TCCGGGACCT GCACCTCAGC 
CAGCAATGCG AACTGCTCAG AAAGCTGCTG ATCAGTGCCC ACGAGCAGCA GACCCAGCAG 
CTGAAACTGT CCCATGACAG GGAAAGCAAA GAGATGCGAG CCCACCAAGC TAAGATTTCT 
ATGGAAAATA GCAAAGCCAT CAGCCAAGAT AAATCCATCA AGAATAAAGC AGAACGGGAA 
AGGAGAGTCA GGGAGTTAAA TAGCAGCAAC ACTAAAAAGT TTCTGGAAGA AAGAAAGAGA 
CTCGCCATGA AGCAGTCCAA AGAAATGGAT CAGTTGAAAA AAGTCCAGCT GGAGCACCTC 
GAATTCCTAG AGAAACAGAA TGAGCAGCTT TTGAAATCCT GTCATGCAGT GTCCCAAACT 
CAAGGCGAAG GAGATGCAGC AGATGGTGAA ATTGGAAGCC GAGATGGACC GCAGACCAGC 
AACAGTTGTG TGAAACTCCA GGACACAAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PLCB4
Entrez Gene ID	101106864
Full Name	phospholipase C beta 4
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PLCB4 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

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