MID1 gene cDNA ORF clone, Macaca mulatta(Rhesus monkey)

mRNA	Protein	Name
XM_001093502.3	XP_001093502.1	E3 ubiquitin-protein ligase Midline-1
XM_015126930.2	XP_014982416.2	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_015126931.2	XP_014982417.2	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_028842300.1	XP_028698133.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
XM_015126930.1	XP_014982416.1	E3 ubiquitin-protein ligase Midline-1
XM_015126929.2	XP_014982415.2	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_028842298.1	XP_028698131.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_015126931.1	XP_014982417.1	E3 ubiquitin-protein ligase Midline-1
XM_028842297.1	XP_028698130.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_015126929.1	XP_014982415.1	E3 ubiquitin-protein ligase Midline-1
XM_001093502.4	XP_001093502.2	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_028842299.1	XP_028698132.1	E3 ubiquitin-protein ligase Midline-1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb22526	XM_015126929.1 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OMb22526	XM_001093502.3 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OMb22526	XM_015126931.1 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OMb22526	XM_015126930.1 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OMb94146	XM_015126930.2 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMb94146	XM_028842297.1 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMb94146	XM_015126931.2 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMb94146	XM_001093502.4 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMb94146	XM_015126929.2 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMb94146	XM_028842298.1 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMb94147	XM_028842300.1 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMb94147	XM_028842299.1 Latest version!	Macaca mulatta midline 1 (MID1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

MID1 ( XM_001093502.3 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001093502.1 Macaca mulatta midline 1 (MID1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMb22526
Clone ID Related Accession (Same CDS sequence)	XM_001093502.3 , XM_015126930.1 , XM_015126931.1 , XM_015126929.1
Accession Version	XM_001093502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 21, 2015 this sequence version replaced XM_001093502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCCACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001093502.1
CDS	574..2577
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDAHTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_001093502.3
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001093502.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCCACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_015126930.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014982416.1 Macaca mulatta midline 1 (MID1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMb22526
Clone ID Related Accession (Same CDS sequence)	XM_001093502.3 , XM_015126930.1 , XM_015126931.1 , XM_015126929.1
Accession Version	XM_015126930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCCACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014982416.1
CDS	480..2483
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDAHTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_015126930.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015126930.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCCACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_015126931.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014982417.1 Macaca mulatta midline 1 (MID1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMb22526
Clone ID Related Accession (Same CDS sequence)	XM_001093502.3 , XM_015126930.1 , XM_015126931.1 , XM_015126929.1
Accession Version	XM_015126931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCCACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014982417.1
CDS	364..2367
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDAHTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_015126931.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015126931.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCCACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_015126929.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014982415.1 Macaca mulatta midline 1 (MID1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMb22526
Clone ID Related Accession (Same CDS sequence)	XM_001093502.3 , XM_015126930.1 , XM_015126931.1 , XM_015126929.1
Accession Version	XM_015126929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCCACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014982415.1
CDS	567..2570
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDAHTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_015126929.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015126929.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCCACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_015126930.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014982416.2 Macaca mulatta midline 1 (MID1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMb94146
Clone ID Related Accession (Same CDS sequence)	XM_015126930.2 , XM_015126931.2 , XM_015126929.2 , XM_028842298.1 , XM_028842297.1 , XM_001093502.4
Accession Version	XM_015126930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015126930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014982416.2
CDS	480..2483
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_015126930.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015126930.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_015126931.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014982417.2 Macaca mulatta midline 1 (MID1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMb94146
Clone ID Related Accession (Same CDS sequence)	XM_015126930.2 , XM_015126931.2 , XM_015126929.2 , XM_028842298.1 , XM_028842297.1 , XM_001093502.4
Accession Version	XM_015126931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015126931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014982417.2
CDS	359..2362
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_015126931.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015126931.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_015126929.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014982415.2 Macaca mulatta midline 1 (MID1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMb94146
Clone ID Related Accession (Same CDS sequence)	XM_015126930.2 , XM_015126931.2 , XM_015126929.2 , XM_028842298.1 , XM_028842297.1 , XM_001093502.4
Accession Version	XM_015126929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015126929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014982415.2
CDS	567..2570
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_015126929.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015126929.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_028842298.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028698131.1 Macaca mulatta midline 1 (MID1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMb94146
Clone ID Related Accession (Same CDS sequence)	XM_015126930.2 , XM_015126931.2 , XM_015126929.2 , XM_028842298.1 , XM_028842297.1 , XM_001093502.4
Accession Version	XM_028842298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028698131.1
CDS	267..2270
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_028842298.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028842298.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_028842297.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028698130.1 Macaca mulatta midline 1 (MID1), transcript variant X3, mRNA.

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CloneID	OMb94146
Clone ID Related Accession (Same CDS sequence)	XM_015126930.2 , XM_015126931.2 , XM_015126929.2 , XM_028842298.1 , XM_028842297.1 , XM_001093502.4
Accession Version	XM_028842297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028698130.1
CDS	166..2169
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_028842297.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028842297.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_001093502.4 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_001093502.2 Macaca mulatta midline 1 (MID1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMb94146
Clone ID Related Accession (Same CDS sequence)	XM_015126930.2 , XM_015126931.2 , XM_015126929.2 , XM_028842298.1 , XM_028842297.1 , XM_001093502.4
Accession Version	XM_001093502.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_001093502.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001093502.2
CDS	187..2190
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_001093502.4
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001093502.4

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTAGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTTATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCACA ACAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAATTCAGT GTGGTCTCCT ACGAACTCCA ATACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAACTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTA CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGACAACT TGACAGTAGA ACGTGATGAA TCATCATCCA AGAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CTATTGGTCT TGCTTATAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCTCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TTCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_028842300.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028698133.1 Macaca mulatta midline 1 (MID1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMb94147
Clone ID Related Accession (Same CDS sequence)	XM_028842300.1 , XM_028842299.1
Accession Version	XM_028842300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTAG CTAAACTCAT CCAAACCTGT CAACATGTTG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTTATTGAGA TCATTCAGCA AAGACGACAA 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCACAACAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCGGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAA TTCAGTGTGG TCTCCTACGA ACTCCAATAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ACTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTACACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ACAACTTGAC AGTAGAACGT GATGAATCAT CATCCAAGAA GAGTCACACG 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCTAT TGGTCTTGCT 
TATAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCT 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTGTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTTCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028698133.1
CDS	567..2456
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_028842300.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028842300.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTAG CTAAACTCAT CCAAACCTGT CAACATGTTG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTTATTGAGA TCATTCAGCA AAGACGACAA 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCACAACAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCGGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAA TTCAGTGTGG TCTCCTACGA ACTCCAATAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ACTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTACACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ACAACTTGAC AGTAGAACGT GATGAATCAT CATCCAAGAA GAGTCACACG 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCTAT TGGTCTTGCT 
TATAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCT 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTGTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTTCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_028842299.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028698132.1 Macaca mulatta midline 1 (MID1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMb94147
Clone ID Related Accession (Same CDS sequence)	XM_028842300.1 , XM_028842299.1
Accession Version	XM_028842299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTAG CTAAACTCAT CCAAACCTGT CAACATGTTG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTTATTGAGA TCATTCAGCA AAGACGACAA 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCACAACAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCGGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAA TTCAGTGTGG TCTCCTACGA ACTCCAATAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ACTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTACACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ACAACTTGAC AGTAGAACGT GATGAATCAT CATCCAAGAA GAGTCACACG 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCTAT TGGTCTTGCT 
TATAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCT 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTGTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTTCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028698132.1
CDS	480..2369
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_028842299.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 1 (MID1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028842299.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGTG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTATCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCACACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTAG CTAAACTCAT CCAAACCTGT CAACATGTTG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTTATTGAGA TCATTCAGCA AAGACGACAA 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCACAACAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCGGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAA TTCAGTGTGG TCTCCTACGA ACTCCAATAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ACTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTACACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ACAACTTGAC AGTAGAACGT GATGAATCAT CATCCAAGAA GAGTCACACG 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCTAT TGGTCTTGCT 
TATAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCT 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTGTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTTCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MID1
Entrez Gene ID	709683
Full Name	midline 1
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)

Mus musculus (house mouse)	Mid1	NP_034927.2
Homo sapiens (human)	MID1	NP_150632.1
Rattus norvegicus (Norway rat)	Mid1	NP_075216.1
Danio rerio (zebrafish)	mid1	XP_002663478.1
Xenopus tropicalis (tropical clawed frog)	mid1	NP_001006876.1
Bos taurus (cattle)	MID1	NP_001179751.1
Gallus gallus (chicken)	MID1	NP_989460.1
	MID1	XP_001093390.1
Canis lupus familiaris (dog)	MID1	XP_548857.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MID1 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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