LOC9270353 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC9270353
Entrez Gene ID	9270353
Full Name	5-oxoprolinase
Gene Type	protein-coding
Organism	Oryza sativa Japonica Group(Japanese rice)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015794771.2	XP_015650257.1	5-oxoprolinase
XM_015794755.2	XP_015650241.1	5-oxoprolinase
XM_015794746.1	XP_015650232.1	5-oxoprolinase
XM_015794746.2	XP_015650232.1	5-oxoprolinase
XM_015794763.1	XP_015650249.1	5-oxoprolinase
XM_015794771.1	XP_015650257.1	5-oxoprolinase
XM_015794755.1	XP_015650241.1	5-oxoprolinase

Pathways from BioSystems
Gene Ontology

The following LOC9270353 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC9270353 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOa25649	XM_015794771.2 Latest version!	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOa25649	XM_015794763.1 Latest version!	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOa25649	XM_015794746.2 Latest version!	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOa25649	XM_015794755.2 Latest version!	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OOa25649	XM_015794746.1	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOa25649	XM_015794771.1	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOa25649	XM_015794755.1	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC9270353 ( XM_015794771.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015650257.1 Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOa25649
Clone ID Related Accession (Same CDS sequence)	XM_015794771.2 , XM_015794755.2 , XM_015794746.1 , XM_015794746.2 , XM_015794763.1 , XM_015794771.1 , XM_015794755.1
Accession Version	XM_015794771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	5-oxoprolinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029256.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015794771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015650257.1
CDS	258..4031
Translation	MGSTEKFRFCIDRGGTFTDIYAEVPGRREGYVMKLLSVDPSNYDDAPIEGIRRILQEFSGERIPRSSKIPTGKIDWIRMGTTVATNALLERKGERIALCVTRGFRDLLQIGNQARPNIFDLKVSKPSNLYEEVVEVDERVELVGGGDGERDDGLSVKGISGELVRVAKPVDVEALKPLLKGLLDKGIRCLAVVLMHSYTYPQHELLIEKLSLEMGFKHVSLSSSLTPMVRAVPRGLTASVDAYLTPVIKEYLSGFMSRFEGGGEQVNVLFMQSDGGLAPERRFSGHKAVLSGPAGGVVGYSQTLFQLETSKPLIGFDMGGTSTDVSRYDGSYEQVLETQIAGAIIQAPQLDINTVAAGGGSKLKFQFGAFKVGPDSVGAHPGPVCYRKGGELAITDANLILGTVIPEYFPSIFGPNEDLPLDYDATKKAFEILAVEINSHRKSQDPSAKDMTVEEIALGFVNVANEAMCRPIRQLTEMKGHDTKNHALACFGGAGPQHACAMARSLGMSELLIHRYCGILSAYGMGLADVIEDLQEPYSAIYNVDSAAEASRRVDLLVKQVKEKLIEQGFGEDSIRTHSYLNLRYEGTDTAIMVKQPERESGSDYADEFVKLFQQEYGFKLLNRKILICDVRVQGVGATNILQPHELTPVSTKPVPESSCRIYFSYGWQETPLYKLQNLGYGHVLKGPAVIMNGNSTVIVEKDCKAIITKYGNIKIEISAAPSSVEVSETVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALFGPDGGLVANAPHVPVHLGAMSSTVRWQLKYWGDNLHEGDVLVTNHPCSGGSHLPDITVVTPVFNEGKVIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVERGVFQEEGIIHLLQSPSYDELTNHKIPGTRKIQDNLSDLHAQVAANQRGITLIKELINQYGLITVQSYMNHVQNNAEEAVREMLKVVASRVEKENGSCVIEDEDYMDDGSVLHLKLTLDSSKGEATFDFEGTSPEVYGNWNAPEAVTAAAVIYCLRCLVDVDIPLNQGCLAPVKILIPKGSFLSPSDKAAVVGGNVLTSQRVTDVVLMAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGASWDGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFSIRENSGGSGFHRGGDGLVREIEFCQPVVVSILSERRVHAPRGLKGGRNGARGANYLVKKDGRRVYLGGKNTVMVNAGEILQILTPGGGGFGSP

Target ORF information:

RefSeq Version	XM_015794771.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015794771.2

ORF Insert Sequence:

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC9270353 ( XM_015794755.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015650241.1 Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOa25649
Clone ID Related Accession (Same CDS sequence)	XM_015794771.2 , XM_015794755.2 , XM_015794746.1 , XM_015794746.2 , XM_015794763.1 , XM_015794771.1 , XM_015794755.1
Accession Version	XM_015794755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	5-oxoprolinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029256.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015794755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015650241.1
CDS	258..4031
Translation	MGSTEKFRFCIDRGGTFTDIYAEVPGRREGYVMKLLSVDPSNYDDAPIEGIRRILQEFSGERIPRSSKIPTGKIDWIRMGTTVATNALLERKGERIALCVTRGFRDLLQIGNQARPNIFDLKVSKPSNLYEEVVEVDERVELVGGGDGERDDGLSVKGISGELVRVAKPVDVEALKPLLKGLLDKGIRCLAVVLMHSYTYPQHELLIEKLSLEMGFKHVSLSSSLTPMVRAVPRGLTASVDAYLTPVIKEYLSGFMSRFEGGGEQVNVLFMQSDGGLAPERRFSGHKAVLSGPAGGVVGYSQTLFQLETSKPLIGFDMGGTSTDVSRYDGSYEQVLETQIAGAIIQAPQLDINTVAAGGGSKLKFQFGAFKVGPDSVGAHPGPVCYRKGGELAITDANLILGTVIPEYFPSIFGPNEDLPLDYDATKKAFEILAVEINSHRKSQDPSAKDMTVEEIALGFVNVANEAMCRPIRQLTEMKGHDTKNHALACFGGAGPQHACAMARSLGMSELLIHRYCGILSAYGMGLADVIEDLQEPYSAIYNVDSAAEASRRVDLLVKQVKEKLIEQGFGEDSIRTHSYLNLRYEGTDTAIMVKQPERESGSDYADEFVKLFQQEYGFKLLNRKILICDVRVQGVGATNILQPHELTPVSTKPVPESSCRIYFSYGWQETPLYKLQNLGYGHVLKGPAVIMNGNSTVIVEKDCKAIITKYGNIKIEISAAPSSVEVSETVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALFGPDGGLVANAPHVPVHLGAMSSTVRWQLKYWGDNLHEGDVLVTNHPCSGGSHLPDITVVTPVFNEGKVIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVERGVFQEEGIIHLLQSPSYDELTNHKIPGTRKIQDNLSDLHAQVAANQRGITLIKELINQYGLITVQSYMNHVQNNAEEAVREMLKVVASRVEKENGSCVIEDEDYMDDGSVLHLKLTLDSSKGEATFDFEGTSPEVYGNWNAPEAVTAAAVIYCLRCLVDVDIPLNQGCLAPVKILIPKGSFLSPSDKAAVVGGNVLTSQRVTDVVLMAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGASWDGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFSIRENSGGSGFHRGGDGLVREIEFCQPVVVSILSERRVHAPRGLKGGRNGARGANYLVKKDGRRVYLGGKNTVMVNAGEILQILTPGGGGFGSP

Target ORF information:

RefSeq Version	XM_015794755.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015794755.2

ORF Insert Sequence:

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC9270353 ( XM_015794746.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015650232.1 Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X1, mRNA.

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CloneID	OOa25649
Clone ID Related Accession (Same CDS sequence)	XM_015794771.2 , XM_015794755.2 , XM_015794746.1 , XM_015794746.2 , XM_015794763.1 , XM_015794771.1 , XM_015794755.1
Accession Version	XM_015794746.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	5-oxoprolinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379174.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015650232.1
CDS	255..4028
Translation	MGSTEKFRFCIDRGGTFTDIYAEVPGRREGYVMKLLSVDPSNYDDAPIEGIRRILQEFSGERIPRSSKIPTGKIDWIRMGTTVATNALLERKGERIALCVTRGFRDLLQIGNQARPNIFDLKVSKPSNLYEEVVEVDERVELVGGGDGERDDGLSVKGISGELVRVAKPVDVEALKPLLKGLLDKGIRCLAVVLMHSYTYPQHELLIEKLSLEMGFKHVSLSSSLTPMVRAVPRGLTASVDAYLTPVIKEYLSGFMSRFEGGGEQVNVLFMQSDGGLAPERRFSGHKAVLSGPAGGVVGYSQTLFQLETSKPLIGFDMGGTSTDVSRYDGSYEQVLETQIAGAIIQAPQLDINTVAAGGGSKLKFQFGAFKVGPDSVGAHPGPVCYRKGGELAITDANLILGTVIPEYFPSIFGPNEDLPLDYDATKKAFEILAVEINSHRKSQDPSAKDMTVEEIALGFVNVANEAMCRPIRQLTEMKGHDTKNHALACFGGAGPQHACAMARSLGMSELLIHRYCGILSAYGMGLADVIEDLQEPYSAIYNVDSAAEASRRVDLLVKQVKEKLIEQGFGEDSIRTHSYLNLRYEGTDTAIMVKQPERESGSDYADEFVKLFQQEYGFKLLNRKILICDVRVQGVGATNILQPHELTPVSTKPVPESSCRIYFSYGWQETPLYKLQNLGYGHVLKGPAVIMNGNSTVIVEKDCKAIITKYGNIKIEISAAPSSVEVSETVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALFGPDGGLVANAPHVPVHLGAMSSTVRWQLKYWGDNLHEGDVLVTNHPCSGGSHLPDITVVTPVFNEGKVIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVERGVFQEEGIIHLLQSPSYDELTNHKIPGTRKIQDNLSDLHAQVAANQRGITLIKELINQYGLITVQSYMNHVQNNAEEAVREMLKVVASRVEKENGSCVIEDEDYMDDGSVLHLKLTLDSSKGEATFDFEGTSPEVYGNWNAPEAVTAAAVIYCLRCLVDVDIPLNQGCLAPVKILIPKGSFLSPSDKAAVVGGNVLTSQRVTDVVLMAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGASWDGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFSIRENSGGSGFHRGGDGLVREIEFCQPVVVSILSERRVHAPRGLKGGRNGARGANYLVKKDGRRVYLGGKNTVMVNAGEILQILTPGGGGFGSP

Target ORF information:

RefSeq Version	XM_015794746.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015794746.1

ORF Insert Sequence:

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC9270353 ( XM_015794746.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015650232.1 Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOa25649
Clone ID Related Accession (Same CDS sequence)	XM_015794771.2 , XM_015794755.2 , XM_015794746.1 , XM_015794746.2 , XM_015794763.1 , XM_015794771.1 , XM_015794755.1
Accession Version	XM_015794746.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	5-oxoprolinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029256.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015794746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015650232.1
CDS	258..4031
Translation	MGSTEKFRFCIDRGGTFTDIYAEVPGRREGYVMKLLSVDPSNYDDAPIEGIRRILQEFSGERIPRSSKIPTGKIDWIRMGTTVATNALLERKGERIALCVTRGFRDLLQIGNQARPNIFDLKVSKPSNLYEEVVEVDERVELVGGGDGERDDGLSVKGISGELVRVAKPVDVEALKPLLKGLLDKGIRCLAVVLMHSYTYPQHELLIEKLSLEMGFKHVSLSSSLTPMVRAVPRGLTASVDAYLTPVIKEYLSGFMSRFEGGGEQVNVLFMQSDGGLAPERRFSGHKAVLSGPAGGVVGYSQTLFQLETSKPLIGFDMGGTSTDVSRYDGSYEQVLETQIAGAIIQAPQLDINTVAAGGGSKLKFQFGAFKVGPDSVGAHPGPVCYRKGGELAITDANLILGTVIPEYFPSIFGPNEDLPLDYDATKKAFEILAVEINSHRKSQDPSAKDMTVEEIALGFVNVANEAMCRPIRQLTEMKGHDTKNHALACFGGAGPQHACAMARSLGMSELLIHRYCGILSAYGMGLADVIEDLQEPYSAIYNVDSAAEASRRVDLLVKQVKEKLIEQGFGEDSIRTHSYLNLRYEGTDTAIMVKQPERESGSDYADEFVKLFQQEYGFKLLNRKILICDVRVQGVGATNILQPHELTPVSTKPVPESSCRIYFSYGWQETPLYKLQNLGYGHVLKGPAVIMNGNSTVIVEKDCKAIITKYGNIKIEISAAPSSVEVSETVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALFGPDGGLVANAPHVPVHLGAMSSTVRWQLKYWGDNLHEGDVLVTNHPCSGGSHLPDITVVTPVFNEGKVIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVERGVFQEEGIIHLLQSPSYDELTNHKIPGTRKIQDNLSDLHAQVAANQRGITLIKELINQYGLITVQSYMNHVQNNAEEAVREMLKVVASRVEKENGSCVIEDEDYMDDGSVLHLKLTLDSSKGEATFDFEGTSPEVYGNWNAPEAVTAAAVIYCLRCLVDVDIPLNQGCLAPVKILIPKGSFLSPSDKAAVVGGNVLTSQRVTDVVLMAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGASWDGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFSIRENSGGSGFHRGGDGLVREIEFCQPVVVSILSERRVHAPRGLKGGRNGARGANYLVKKDGRRVYLGGKNTVMVNAGEILQILTPGGGGFGSP

Target ORF information:

RefSeq Version	XM_015794746.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015794746.2

ORF Insert Sequence:

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC9270353 ( XM_015794763.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015650249.1 Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOa25649
Clone ID Related Accession (Same CDS sequence)	XM_015794771.2 , XM_015794755.2 , XM_015794746.1 , XM_015794746.2 , XM_015794763.1 , XM_015794771.1 , XM_015794755.1
Accession Version	XM_015794763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	5-oxoprolinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379174.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015650249.1
CDS	255..4028
Translation	MGSTEKFRFCIDRGGTFTDIYAEVPGRREGYVMKLLSVDPSNYDDAPIEGIRRILQEFSGERIPRSSKIPTGKIDWIRMGTTVATNALLERKGERIALCVTRGFRDLLQIGNQARPNIFDLKVSKPSNLYEEVVEVDERVELVGGGDGERDDGLSVKGISGELVRVAKPVDVEALKPLLKGLLDKGIRCLAVVLMHSYTYPQHELLIEKLSLEMGFKHVSLSSSLTPMVRAVPRGLTASVDAYLTPVIKEYLSGFMSRFEGGGEQVNVLFMQSDGGLAPERRFSGHKAVLSGPAGGVVGYSQTLFQLETSKPLIGFDMGGTSTDVSRYDGSYEQVLETQIAGAIIQAPQLDINTVAAGGGSKLKFQFGAFKVGPDSVGAHPGPVCYRKGGELAITDANLILGTVIPEYFPSIFGPNEDLPLDYDATKKAFEILAVEINSHRKSQDPSAKDMTVEEIALGFVNVANEAMCRPIRQLTEMKGHDTKNHALACFGGAGPQHACAMARSLGMSELLIHRYCGILSAYGMGLADVIEDLQEPYSAIYNVDSAAEASRRVDLLVKQVKEKLIEQGFGEDSIRTHSYLNLRYEGTDTAIMVKQPERESGSDYADEFVKLFQQEYGFKLLNRKILICDVRVQGVGATNILQPHELTPVSTKPVPESSCRIYFSYGWQETPLYKLQNLGYGHVLKGPAVIMNGNSTVIVEKDCKAIITKYGNIKIEISAAPSSVEVSETVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALFGPDGGLVANAPHVPVHLGAMSSTVRWQLKYWGDNLHEGDVLVTNHPCSGGSHLPDITVVTPVFNEGKVIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVERGVFQEEGIIHLLQSPSYDELTNHKIPGTRKIQDNLSDLHAQVAANQRGITLIKELINQYGLITVQSYMNHVQNNAEEAVREMLKVVASRVEKENGSCVIEDEDYMDDGSVLHLKLTLDSSKGEATFDFEGTSPEVYGNWNAPEAVTAAAVIYCLRCLVDVDIPLNQGCLAPVKILIPKGSFLSPSDKAAVVGGNVLTSQRVTDVVLMAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGASWDGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFSIRENSGGSGFHRGGDGLVREIEFCQPVVVSILSERRVHAPRGLKGGRNGARGANYLVKKDGRRVYLGGKNTVMVNAGEILQILTPGGGGFGSP

Target ORF information:

RefSeq Version	XM_015794763.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015794763.1

ORF Insert Sequence:

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC9270353 ( XM_015794771.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015650257.1 Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X4, mRNA.

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CloneID	OOa25649
Clone ID Related Accession (Same CDS sequence)	XM_015794771.2 , XM_015794755.2 , XM_015794746.1 , XM_015794746.2 , XM_015794763.1 , XM_015794771.1 , XM_015794755.1
Accession Version	XM_015794771.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	5-oxoprolinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379174.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015650257.1
CDS	255..4028
Translation	MGSTEKFRFCIDRGGTFTDIYAEVPGRREGYVMKLLSVDPSNYDDAPIEGIRRILQEFSGERIPRSSKIPTGKIDWIRMGTTVATNALLERKGERIALCVTRGFRDLLQIGNQARPNIFDLKVSKPSNLYEEVVEVDERVELVGGGDGERDDGLSVKGISGELVRVAKPVDVEALKPLLKGLLDKGIRCLAVVLMHSYTYPQHELLIEKLSLEMGFKHVSLSSSLTPMVRAVPRGLTASVDAYLTPVIKEYLSGFMSRFEGGGEQVNVLFMQSDGGLAPERRFSGHKAVLSGPAGGVVGYSQTLFQLETSKPLIGFDMGGTSTDVSRYDGSYEQVLETQIAGAIIQAPQLDINTVAAGGGSKLKFQFGAFKVGPDSVGAHPGPVCYRKGGELAITDANLILGTVIPEYFPSIFGPNEDLPLDYDATKKAFEILAVEINSHRKSQDPSAKDMTVEEIALGFVNVANEAMCRPIRQLTEMKGHDTKNHALACFGGAGPQHACAMARSLGMSELLIHRYCGILSAYGMGLADVIEDLQEPYSAIYNVDSAAEASRRVDLLVKQVKEKLIEQGFGEDSIRTHSYLNLRYEGTDTAIMVKQPERESGSDYADEFVKLFQQEYGFKLLNRKILICDVRVQGVGATNILQPHELTPVSTKPVPESSCRIYFSYGWQETPLYKLQNLGYGHVLKGPAVIMNGNSTVIVEKDCKAIITKYGNIKIEISAAPSSVEVSETVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALFGPDGGLVANAPHVPVHLGAMSSTVRWQLKYWGDNLHEGDVLVTNHPCSGGSHLPDITVVTPVFNEGKVIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVERGVFQEEGIIHLLQSPSYDELTNHKIPGTRKIQDNLSDLHAQVAANQRGITLIKELINQYGLITVQSYMNHVQNNAEEAVREMLKVVASRVEKENGSCVIEDEDYMDDGSVLHLKLTLDSSKGEATFDFEGTSPEVYGNWNAPEAVTAAAVIYCLRCLVDVDIPLNQGCLAPVKILIPKGSFLSPSDKAAVVGGNVLTSQRVTDVVLMAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGASWDGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFSIRENSGGSGFHRGGDGLVREIEFCQPVVVSILSERRVHAPRGLKGGRNGARGANYLVKKDGRRVYLGGKNTVMVNAGEILQILTPGGGGFGSP

Target ORF information:

RefSeq Version	XM_015794771.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015794771.1

ORF Insert Sequence:

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC9270353 ( XM_015794755.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015650241.1 Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X2, mRNA.

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CloneID	OOa25649
Clone ID Related Accession (Same CDS sequence)	XM_015794771.2 , XM_015794755.2 , XM_015794746.1 , XM_015794746.2 , XM_015794763.1 , XM_015794771.1 , XM_015794755.1
Accession Version	XM_015794755.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	5-oxoprolinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379174.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015650241.1
CDS	255..4028
Translation	MGSTEKFRFCIDRGGTFTDIYAEVPGRREGYVMKLLSVDPSNYDDAPIEGIRRILQEFSGERIPRSSKIPTGKIDWIRMGTTVATNALLERKGERIALCVTRGFRDLLQIGNQARPNIFDLKVSKPSNLYEEVVEVDERVELVGGGDGERDDGLSVKGISGELVRVAKPVDVEALKPLLKGLLDKGIRCLAVVLMHSYTYPQHELLIEKLSLEMGFKHVSLSSSLTPMVRAVPRGLTASVDAYLTPVIKEYLSGFMSRFEGGGEQVNVLFMQSDGGLAPERRFSGHKAVLSGPAGGVVGYSQTLFQLETSKPLIGFDMGGTSTDVSRYDGSYEQVLETQIAGAIIQAPQLDINTVAAGGGSKLKFQFGAFKVGPDSVGAHPGPVCYRKGGELAITDANLILGTVIPEYFPSIFGPNEDLPLDYDATKKAFEILAVEINSHRKSQDPSAKDMTVEEIALGFVNVANEAMCRPIRQLTEMKGHDTKNHALACFGGAGPQHACAMARSLGMSELLIHRYCGILSAYGMGLADVIEDLQEPYSAIYNVDSAAEASRRVDLLVKQVKEKLIEQGFGEDSIRTHSYLNLRYEGTDTAIMVKQPERESGSDYADEFVKLFQQEYGFKLLNRKILICDVRVQGVGATNILQPHELTPVSTKPVPESSCRIYFSYGWQETPLYKLQNLGYGHVLKGPAVIMNGNSTVIVEKDCKAIITKYGNIKIEISAAPSSVEVSETVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALFGPDGGLVANAPHVPVHLGAMSSTVRWQLKYWGDNLHEGDVLVTNHPCSGGSHLPDITVVTPVFNEGKVIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVERGVFQEEGIIHLLQSPSYDELTNHKIPGTRKIQDNLSDLHAQVAANQRGITLIKELINQYGLITVQSYMNHVQNNAEEAVREMLKVVASRVEKENGSCVIEDEDYMDDGSVLHLKLTLDSSKGEATFDFEGTSPEVYGNWNAPEAVTAAAVIYCLRCLVDVDIPLNQGCLAPVKILIPKGSFLSPSDKAAVVGGNVLTSQRVTDVVLMAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGASWDGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFSIRENSGGSGFHRGGDGLVREIEFCQPVVVSILSERRVHAPRGLKGGRNGARGANYLVKKDGRRVYLGGKNTVMVNAGEILQILTPGGGGFGSP

Target ORF information:

RefSeq Version	XM_015794755.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 5-oxoprolinase (LOC9270353), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015794755.1

ORF Insert Sequence:

ATGGGTAGCA CGGAGAAATT CAGATTCTGC ATTGACAGGG GCGGCACATT CACCGACATC 
TACGCAGAGG TGCCCGGGAG GAGGGAAGGC TACGTGATGA AACTTCTCTC CGTCGACCCG 
TCGAACTACG ACGACGCCCC CATCGAAGGG ATCAGGAGGA TCCTCCAGGA GTTCTCCGGG 
GAGAGGATCC CGCGGTCGTC CAAGATTCCC ACGGGCAAGA TTGATTGGAT TCGGATGGGC 
ACCACGGTGG CCACGAATGC GCTTCTTGAG AGGAAGGGTG AAAGGATCGC GCTGTGTGTC 
ACCCGGGGAT TCAGGGATTT GCTTCAGATT GGTAACCAGG CTCGGCCGAA CATATTCGAT 
CTCAAGGTGT CAAAGCCGTC GAATTTGTAT GAGGAGGTGG TTGAGGTTGA TGAGAGGGTT 
GAACTTGTTG GTGGTGGTGA TGGTGAGAGG GATGATGGTT TATCTGTTAA AGGGATCTCA 
GGGGAATTGG TCAGGGTGGC GAAGCCGGTT GATGTAGAAG CATTGAAGCC TTTGTTGAAA 
GGTTTGCTTG ACAAAGGAAT AAGATGTTTG GCAGTGGTTT TAATGCACTC ATATACTTAT 
CCTCAGCATG AGCTTCTCAT TGAGAAATTA TCTCTTGAGA TGGGATTCAA GCATGTATCG 
TTGTCTTCAT CATTGACGCC GATGGTCCGT GCTGTGCCTC GTGGCTTGAC AGCCAGTGTG 
GATGCTTATC TCACACCAGT CATCAAAGAG TACCTATCAG GATTCATGTC CAGGTTTGAA 
GGGGGAGGTG AGCAGGTGAA TGTGCTGTTT ATGCAGTCAG ATGGTGGTCT GGCACCAGAG 
AGGAGATTCT CCGGGCACAA AGCAGTGTTG TCAGGTCCTG CAGGTGGTGT GGTTGGCTAC 
TCTCAGACCT TGTTTCAACT TGAGACATCA AAACCGCTGA TTGGATTTGA CATGGGTGGT 
ACATCCACAG ATGTTAGCCG CTACGATGGA AGCTATGAAC AAGTTCTGGA GACCCAAATT 
GCTGGGGCAA TAATACAAGC TCCCCAGCTT GACATAAACA CGGTGGCTGC TGGTGGTGGA 
TCGAAGCTTA AGTTTCAGTT TGGAGCTTTC AAGGTTGGGC CAGATTCTGT TGGAGCACAT 
CCTGGTCCTG TATGCTATAG AAAAGGTGGT GAGCTGGCAA TAACTGATGC CAATTTGATT 
CTGGGAACTG TTATTCCTGA GTACTTCCCA TCCATATTTG GTCCTAACGA AGATTTACCC 
CTTGATTATG ACGCTACTAA AAAGGCATTT GAGATTCTTG CTGTTGAGAT AAACTCTCAC 
CGAAAGAGTC AGGATCCATC AGCAAAGGAC ATGACGGTTG AGGAGATTGC CCTCGGTTTT 
GTTAATGTTG CAAATGAGGC GATGTGCCGA CCTATAAGGC AGTTGACCGA AATGAAGGGG 
CATGACACTA AGAACCATGC TCTCGCATGC TTTGGTGGTG CTGGTCCTCA ACATGCATGT 
GCTATGGCAA GATCCCTTGG CATGTCTGAG TTACTTATTC ATCGCTACTG TGGCATATTG 
AGTGCATATG GAATGGGCCT TGCTGATGTT ATTGAAGATT TGCAAGAGCC ATACTCTGCT 
ATTTATAATG TTGATTCTGC TGCAGAGGCA TCTCGAAGAG TAGATCTTTT AGTTAAGCAG 
GTGAAAGAAA AGTTAATAGA GCAGGGTTTT GGAGAGGACA GCATCAGGAC ACATTCATAC 
TTGAACTTGA GGTACGAGGG AACCGATACA GCAATCATGG TTAAACAACC AGAGAGGGAA 
TCTGGAAGTG ATTATGCTGA TGAGTTTGTG AAACTGTTTC AGCAAGAGTA TGGCTTCAAA 
TTATTAAACA GAAAAATACT CATATGTGAT GTGAGGGTTC AGGGAGTTGG TGCTACAAAT 
ATCTTGCAGC CTCATGAACT GACACCAGTA TCAACCAAAC CTGTGCCAGA AAGTTCATGT 
CGAATTTATT TTTCATACGG ATGGCAGGAA ACACCACTTT ACAAGCTTCA GAATTTGGGC 
TATGGGCATG TATTGAAGGG TCCTGCAGTT ATAATGAATG GGAACAGTAC AGTGATAGTA 
GAAAAAGACT GTAAAGCTAT CATCACCAAG TATGGTAACA TAAAAATTGA GATAAGTGCT 
GCTCCAAGCA GTGTAGAAGT ATCAGAGACA GTTGCTGATG TGGTCCAACT TTCTATCTTC 
AATCATCGAT TCATGGGTAT TGCTGAACAG ATGGGTCGAA CACTTCAAAG AACTTCTATT 
TCTACCAATA TAAAGGAAAG ACTGGACTTT TCTTGTGCTC TCTTTGGTCC GGATGGTGGC 
CTTGTCGCAA ATGCCCCTCA TGTCCCTGTG CATTTGGGAG CCATGTCTAG CACCGTGCGT 
TGGCAACTCA AATATTGGGG TGATAATCTC CATGAGGGTG ATGTTCTTGT AACGAATCAT 
CCATGTTCTG GGGGTAGCCA TCTTCCAGAC ATAACAGTTG TCACACCTGT TTTCAATGAA 
GGTAAAGTTA TCTTCTTTGT TGCTAGTAGA GGTCACCATG CAGAGATCGG TGGTATCACC 
CCAGGAAGCA TGCCTCCTTT CTCAAAAAGC ATCTGGGAGG AAGGTGCTGC CATCAAAGCA 
TTTAAACTTG TTGAGAGAGG TGTTTTCCAA GAGGAAGGAA TAATTCACTT GCTACAGTCA 
CCGTCCTATG ATGAACTTAC AAACCATAAG ATTCCAGGAA CACGTAAGAT CCAAGATAAT 
CTTTCTGACC TCCATGCTCA GGTGGCAGCA AACCAGCGGG GAATAACACT TATCAAAGAA 
CTGATAAATC AGTATGGTTT GATCACTGTT CAATCTTATA TGAATCATGT CCAAAACAAT 
GCTGAAGAAG CTGTTAGAGA AATGCTTAAG GTAGTTGCAT CGAGAGTTGA AAAGGAGAAT 
GGGTCTTGTG TCATCGAAGA TGAAGATTAC ATGGATGATG GTTCTGTGCT CCATTTGAAG 
CTCACCCTTG ATTCAAGTAA AGGCGAAGCA ACCTTTGACT TTGAAGGCAC CAGTCCTGAG 
GTGTATGGTA ACTGGAATGC TCCTGAAGCA GTAACAGCGG CTGCTGTGAT ATACTGCTTA 
CGGTGCTTGG TCGACGTTGA TATACCTCTA AATCAAGGCT GCCTAGCTCC TGTGAAGATC 
CTAATTCCTA AAGGCTCTTT CCTTTCTCCA AGTGACAAAG CTGCAGTGGT TGGTGGTAAT 
GTATTGACCT CTCAGAGAGT GACGGACGTA GTCCTAATGG CTTTCCAAGC ATGCGCCTGC 
TCCCAGGGTT GCATGAACAA TTTGACATTT GGAGACGACA CCTTCGGTTA CTATGAGACC 
ATTGGAGGCG GTTCAGGAGC TGGGGCGAGT TGGGATGGTA CAAGTGGTGT TCAGTGTCAC 
ATGACAAACA CAAGGATGAC TGACCCAGAG ATCTTTGAGC AGCGATACCC TGTTCTTTTA 
CATAGATTTA GCATCAGGGA GAACAGTGGA GGTTCTGGTT TCCACAGAGG TGGTGATGGC 
CTTGTGAGAG AGATTGAATT TTGCCAGCCT GTTGTTGTGA GCATTCTTTC AGAGAGAAGG 
GTGCATGCTC CCAGGGGACT GAAGGGAGGA AGAAATGGGG CCCGTGGTGC AAACTATCTC 
GTCAAGAAAG ATGGTCGTAG AGTTTACCTT GGAGGGAAGA ACACTGTTAT GGTTAATGCT 
GGTGAGATTC TTCAGATTCT CACTCCTGGT GGTGGTGGTT TTGGCTCTCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC9270353 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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