LOC4332758 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC4332758
Entrez Gene ID	4332758
Full Name	valine--tRNA ligase, mitochondrial 1
Gene Type	protein-coding
Organism	Oryza sativa Japonica Group(Japanese rice)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015777288.2	XP_015632774.1	valine--tRNA ligase, mitochondrial 1 isoform X1
XM_015777289.2	XP_015632775.1	valine--tRNA ligase, mitochondrial 1 isoform X2
XM_015777290.2	XP_015632776.1	valine--tRNA ligase, mitochondrial 1 isoform X3
XM_015777289.1	XP_015632775.1	valine--tRNA ligase, mitochondrial 1 isoform X2
XM_026024198.1	XP_025879983.1	valine--tRNA ligase, mitochondrial 1 isoform X3
XM_015777288.1	XP_015632774.1	valine--tRNA ligase, mitochondrial 1 isoform X1
XM_015777290.1	XP_015632776.1	valine--tRNA ligase, mitochondrial 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC4332758 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC4332758 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOa30946	XM_015777288.2 Latest version!	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOa30947	XM_015777289.2 Latest version!	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOa30948	XM_015777290.2 Latest version!	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOa30948	XM_026024198.1 Latest version!	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OOa30947	XM_015777289.1	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOa30946	XM_015777288.1	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOa30948	XM_015777290.1	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC4332758 ( XM_015777288.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632774.1 Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOa30946
Clone ID Related Accession (Same CDS sequence)	XM_015777288.2 , XM_015777288.1
Accession Version	XM_015777288.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	valine--tRNA ligase, mitochondrial 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015777288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGC AGCCCGAGGT GCATGGTCCG GAGAAGGCTC CGGAGAAGCA ACTTGATGAG 
AAAACGTTAG AACGGAAACT GAGGAAAAAT CAGAAGGCTA AAGAGAAAGA AGAGAAAAGA 
CTTAAAGCAA AACAAAAGGA GGCAGCTATG CTTCAGGCAC AGCCAGCTTT AGATGTACTC 
AAGAAGGTTG AGAAGAAACA TAGGGGAAAA GCTGTGGAGG ATGAAAATCC TGAAGATTTT 
ATTGATCAAG ACACTCCTAA TGGGCAGAAG AAACTGCTTG CACCTCAAAT GGCCAATCAG 
TATTGCCCAA GCACGGTTGA GAAATCATGG TATGCCTGGT GGGAATCATC AGGATATTTT 
AGGGCAGATT CTGCAAGCAC AAAGCCACCA TTTGTTATAG TTATGCCACC TCCAAATGTG 
ACTGGAGCTC TTCACATAGG CCATGCTCTT ACAGTGGCTA TAGAGGATGC GATGATACGA 
TGGCGGAGGA TGTCAGGTTA TAATGCTCTA TGGGTCCCTG GAGTGGACCA TGCTGGCATT 
GCTACACAGG TTGTAGTAGA AAAAAAGCTT ATGCGTGAAA GGAATCTGAC AAGGCATGAC 
ATAGGGCCTG ACAATTTTGT TTGTGAAGTT CTCAAATGGA AAGAGCGGTA TGGTGGTACT 
ATATTGAATC AATTGCACCG CCTTGGGGCC TCACTTGATT GGTCTCGTGA GGCTTTTACA 
ATGGATGAGC AAAGGTCAAA TGCTGTAACT GAAGCTTTTG TAAGGTTACA CAAGGAAGGC 
CTGATATATA GAGATAACCG CCTTGTGAAT TGGGACTGCA CACTTCTGAC TTCAATTTCT 
GAGATAGAGG TTGATCATAT TGATCTTAAA GAAGAAACAA TGTTAAAGAT CCCTGGTTAT 
GCTACTCCTG TACAATTTGG TGTGTTGATA TCTTTTGCCT ATCCTCTTGA AGAAGGGCTA 
GGTGAGATTG TTGTTGCAAC AACTCGAATT GAAACAATGC TTGGTGATAC AGCAATAGCT 
GTTCATCCTG AGGATAAAAG GTATATGCAT CTGCATGGAC GGTATGCAGT CCACCCCTTC 
AATGGCCGAA AACTCAAAAT AATCTGTGAT GCTGAGATAG TTGATCCCAG TTTCGGTACT 
GGGGCTGTCA AGATTGCACC TGCACATGAC CCAAATGACT TTGAGGTTGG AAGACGGAAT 
AACCTTCAGT TCATTAATAT CTTGACAGAT GATGGGAAAA TAAACAGCAA TGGGGGTGCA 
CAATTTGAAG GAATGCCAAG ATTCACTGCT CGAATTTGCA TTATTGAAGC ACTGAAGGCA 
AAGGGCTTGT ACAAGGGTGC AAAGAATACC GAAACGAGTT TGGGCATTTG TTCAAGAACT 
AACGACATAG TGGAGCCTAT GATAAAATCC CCGCAGTGGT TTGTTAATTG TAACACCATG 
GCCAAGGTAG CCCTTGATGC TGTAAGGTCT AAGAGGATTG AGATCATTCC CCCGCAGTAC 
GAACAAGACT GGTATAGATG GCTTGAAAAT ATACGTGATT GGTGTGTTTC AAGGCAACAT 
TGGTGGGGAC ACCGTGTACC TGCATGGTAT GTGACACTAG AAGATGACCA GGAGAAAACT 
CTAGGTTCTG ACAATGACCG CTGGATAGTC GCCAAAAGTG AAAGTGCTGC AAGCGTTGAG 
GCACAGAAAA GTTATCCAGG AAAGAAATTC ATCCTAAATC AGGATCCTGA TGTGCTAGAC 
ACATGGTTTT CATCTGCTCT CTTCCCCTTA GCAGTACTTG GTTGGCCAGA TGATACTGCT 
GACCTCCGTT CTTTCTACCC TACTTCAGTT CTAGAAACTG GACTTGATAT TCTCTTCTGT 
TGGGTAGCAC GGATGGTGAT GATGGGAACA CAACTTGGTG GTGATGTGCC GTTTCAGAAG 
GTTTATTTGC ATCCTATTGT ATGCGACACA CATGGCCGAA AAATGTGCAA GTCACTTGGT 
AATGTCATTG ATCCTCTTGA GGTGATAAAT GGCATGACAC TCGAGGGTCT TGTAAAACGT 
CTGGAAGAAG GTAATCTGGA TCCAGATGAA TTAAACCTTG AACGGAAGCT GACAGATTAT 
CCTGATGGAA TTGCTGAATG TGGTACTGAT GCCCTCCGCT TTGCACTGAT TTCTTACACT 
TCTCAGTCTG ACAGGATAAA TCTTGATATC AAGAGAGTGG TTGGATACCG ACAATGGTGC 
AATAAATTGT GGAATGCCAT CCGTTTTGCC ATGGGCAAGC TTGGTGTTCA CTACAGTCCA 
CCTGCAACTG TCGACGTGTC TATCATGCCA CCAATCTGCA AGTGGATACT CTCAGCACTA 
AACAAGGCTA CCGGCAAAAC TGTTACCTCT TTAGAAGCAT ACAAATTCTC TGATGCAACA 
TCTGCTATAT ACTCTTGGTG GCAATACCAG CTATGTGATG TATTTATAGA AGCTATAAAA 
CCTTACTTCT TCAACGAACC TCAAGAGTTT GAATCTGCAA GAGTCGCCTC CAGAGATACT 
CTATGGGTTT GCCTGGAGAC TGGTTTGCGC TTGCTCCACC CATTCATGCC TTACATAACA 
GAAGAGCTAT GGCAGCATCT TCCACAACCC AAATATTCTT GTAGGCAAGA TTCAATTATG 
ATTTCGGAAT ATCCATCTCT CGTTGAGGAA TGGACTAATG ACAATCTTGA AAATGAAATG 
GACATTGTCT TAGACACCGT GAACAAAATA AGGTCTTTGA AGACACGAAC AGAAAGAAAA 
GAAAGACGTC CTGCTTTTGC ACTCTGCCGA AGCCAAGATG TCACTGCCAC TATTCAATGC 
CATCAATCTC TAATTGTGTG TCTTTCTTCC GTATCATTTC TTAAGATCCT GACAGAGAAT 
GACGAAACTC CAGCTGATTG TGCAATCGCT GTTGTTAACA AAGATTTGTC GGTGTACCTT 
AAGCTTCAAG GAGCCATAAA TGCAGAGGCT GAGCGTGAAA AGCTAAGGAA GAAGAGAGAT 
GGAATCCAAA AGCTACACCA TGCTGTGACA CACATGATGG ATGCTTCTGG TTATCGCGAG 
AAGGCACCTC AAAGTGTCCA GGAAGGGGAT ATGAGAAAGC ACACTGCTTT GTTGCGGGAA 
CTGGAGGGCA TAAGTGAAGC TGAGAAGAAA CTAGATGCCA AAACCGATAA TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632774.1
CDS	433..3609
Translation	MEKQPEVHGPEKAPEKQLDEKTLERKLRKNQKAKEKEEKRLKAKQKEAAMLQAQPALDVLKKVEKKHRGKAVEDENPEDFIDQDTPNGQKKLLAPQMANQYCPSTVEKSWYAWWESSGYFRADSASTKPPFVIVMPPPNVTGALHIGHALTVAIEDAMIRWRRMSGYNALWVPGVDHAGIATQVVVEKKLMRERNLTRHDIGPDNFVCEVLKWKERYGGTILNQLHRLGASLDWSREAFTMDEQRSNAVTEAFVRLHKEGLIYRDNRLVNWDCTLLTSISEIEVDHIDLKEETMLKIPGYATPVQFGVLISFAYPLEEGLGEIVVATTRIETMLGDTAIAVHPEDKRYMHLHGRYAVHPFNGRKLKIICDAEIVDPSFGTGAVKIAPAHDPNDFEVGRRNNLQFINILTDDGKINSNGGAQFEGMPRFTARICIIEALKAKGLYKGAKNTETSLGICSRTNDIVEPMIKSPQWFVNCNTMAKVALDAVRSKRIEIIPPQYEQDWYRWLENIRDWCVSRQHWWGHRVPAWYVTLEDDQEKTLGSDNDRWIVAKSESAASVEAQKSYPGKKFILNQDPDVLDTWFSSALFPLAVLGWPDDTADLRSFYPTSVLETGLDILFCWVARMVMMGTQLGGDVPFQKVYLHPIVCDTHGRKMCKSLGNVIDPLEVINGMTLEGLVKRLEEGNLDPDELNLERKLTDYPDGIAECGTDALRFALISYTSQSDRINLDIKRVVGYRQWCNKLWNAIRFAMGKLGVHYSPPATVDVSIMPPICKWILSALNKATGKTVTSLEAYKFSDATSAIYSWWQYQLCDVFIEAIKPYFFNEPQEFESARVASRDTLWVCLETGLRLLHPFMPYITEELWQHLPQPKYSCRQDSIMISEYPSLVEEWTNDNLENEMDIVLDTVNKIRSLKTRTERKERRPAFALCRSQDVTATIQCHQSLIVCLSSVSFLKILTENDETPADCAIAVVNKDLSVYLKLQGAINAEAEREKLRKKRDGIQKLHHAVTHMMDASGYREKAPQSVQEGDMRKHTALLRELEGISEAEKKLDAKTDNI

Target ORF information:

RefSeq Version	XM_015777288.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777288.2

ORF Insert Sequence:

ATGGAAAAGC AGCCCGAGGT GCATGGTCCG GAGAAGGCTC CGGAGAAGCA ACTTGATGAG 
AAAACGTTAG AACGGAAACT GAGGAAAAAT CAGAAGGCTA AAGAGAAAGA AGAGAAAAGA 
CTTAAAGCAA AACAAAAGGA GGCAGCTATG CTTCAGGCAC AGCCAGCTTT AGATGTACTC 
AAGAAGGTTG AGAAGAAACA TAGGGGAAAA GCTGTGGAGG ATGAAAATCC TGAAGATTTT 
ATTGATCAAG ACACTCCTAA TGGGCAGAAG AAACTGCTTG CACCTCAAAT GGCCAATCAG 
TATTGCCCAA GCACGGTTGA GAAATCATGG TATGCCTGGT GGGAATCATC AGGATATTTT 
AGGGCAGATT CTGCAAGCAC AAAGCCACCA TTTGTTATAG TTATGCCACC TCCAAATGTG 
ACTGGAGCTC TTCACATAGG CCATGCTCTT ACAGTGGCTA TAGAGGATGC GATGATACGA 
TGGCGGAGGA TGTCAGGTTA TAATGCTCTA TGGGTCCCTG GAGTGGACCA TGCTGGCATT 
GCTACACAGG TTGTAGTAGA AAAAAAGCTT ATGCGTGAAA GGAATCTGAC AAGGCATGAC 
ATAGGGCCTG ACAATTTTGT TTGTGAAGTT CTCAAATGGA AAGAGCGGTA TGGTGGTACT 
ATATTGAATC AATTGCACCG CCTTGGGGCC TCACTTGATT GGTCTCGTGA GGCTTTTACA 
ATGGATGAGC AAAGGTCAAA TGCTGTAACT GAAGCTTTTG TAAGGTTACA CAAGGAAGGC 
CTGATATATA GAGATAACCG CCTTGTGAAT TGGGACTGCA CACTTCTGAC TTCAATTTCT 
GAGATAGAGG TTGATCATAT TGATCTTAAA GAAGAAACAA TGTTAAAGAT CCCTGGTTAT 
GCTACTCCTG TACAATTTGG TGTGTTGATA TCTTTTGCCT ATCCTCTTGA AGAAGGGCTA 
GGTGAGATTG TTGTTGCAAC AACTCGAATT GAAACAATGC TTGGTGATAC AGCAATAGCT 
GTTCATCCTG AGGATAAAAG GTATATGCAT CTGCATGGAC GGTATGCAGT CCACCCCTTC 
AATGGCCGAA AACTCAAAAT AATCTGTGAT GCTGAGATAG TTGATCCCAG TTTCGGTACT 
GGGGCTGTCA AGATTGCACC TGCACATGAC CCAAATGACT TTGAGGTTGG AAGACGGAAT 
AACCTTCAGT TCATTAATAT CTTGACAGAT GATGGGAAAA TAAACAGCAA TGGGGGTGCA 
CAATTTGAAG GAATGCCAAG ATTCACTGCT CGAATTTGCA TTATTGAAGC ACTGAAGGCA 
AAGGGCTTGT ACAAGGGTGC AAAGAATACC GAAACGAGTT TGGGCATTTG TTCAAGAACT 
AACGACATAG TGGAGCCTAT GATAAAATCC CCGCAGTGGT TTGTTAATTG TAACACCATG 
GCCAAGGTAG CCCTTGATGC TGTAAGGTCT AAGAGGATTG AGATCATTCC CCCGCAGTAC 
GAACAAGACT GGTATAGATG GCTTGAAAAT ATACGTGATT GGTGTGTTTC AAGGCAACAT 
TGGTGGGGAC ACCGTGTACC TGCATGGTAT GTGACACTAG AAGATGACCA GGAGAAAACT 
CTAGGTTCTG ACAATGACCG CTGGATAGTC GCCAAAAGTG AAAGTGCTGC AAGCGTTGAG 
GCACAGAAAA GTTATCCAGG AAAGAAATTC ATCCTAAATC AGGATCCTGA TGTGCTAGAC 
ACATGGTTTT CATCTGCTCT CTTCCCCTTA GCAGTACTTG GTTGGCCAGA TGATACTGCT 
GACCTCCGTT CTTTCTACCC TACTTCAGTT CTAGAAACTG GACTTGATAT TCTCTTCTGT 
TGGGTAGCAC GGATGGTGAT GATGGGAACA CAACTTGGTG GTGATGTGCC GTTTCAGAAG 
GTTTATTTGC ATCCTATTGT ATGCGACACA CATGGCCGAA AAATGTGCAA GTCACTTGGT 
AATGTCATTG ATCCTCTTGA GGTGATAAAT GGCATGACAC TCGAGGGTCT TGTAAAACGT 
CTGGAAGAAG GTAATCTGGA TCCAGATGAA TTAAACCTTG AACGGAAGCT GACAGATTAT 
CCTGATGGAA TTGCTGAATG TGGTACTGAT GCCCTCCGCT TTGCACTGAT TTCTTACACT 
TCTCAGTCTG ACAGGATAAA TCTTGATATC AAGAGAGTGG TTGGATACCG ACAATGGTGC 
AATAAATTGT GGAATGCCAT CCGTTTTGCC ATGGGCAAGC TTGGTGTTCA CTACAGTCCA 
CCTGCAACTG TCGACGTGTC TATCATGCCA CCAATCTGCA AGTGGATACT CTCAGCACTA 
AACAAGGCTA CCGGCAAAAC TGTTACCTCT TTAGAAGCAT ACAAATTCTC TGATGCAACA 
TCTGCTATAT ACTCTTGGTG GCAATACCAG CTATGTGATG TATTTATAGA AGCTATAAAA 
CCTTACTTCT TCAACGAACC TCAAGAGTTT GAATCTGCAA GAGTCGCCTC CAGAGATACT 
CTATGGGTTT GCCTGGAGAC TGGTTTGCGC TTGCTCCACC CATTCATGCC TTACATAACA 
GAAGAGCTAT GGCAGCATCT TCCACAACCC AAATATTCTT GTAGGCAAGA TTCAATTATG 
ATTTCGGAAT ATCCATCTCT CGTTGAGGAA TGGACTAATG ACAATCTTGA AAATGAAATG 
GACATTGTCT TAGACACCGT GAACAAAATA AGGTCTTTGA AGACACGAAC AGAAAGAAAA 
GAAAGACGTC CTGCTTTTGC ACTCTGCCGA AGCCAAGATG TCACTGCCAC TATTCAATGC 
CATCAATCTC TAATTGTGTG TCTTTCTTCC GTATCATTTC TTAAGATCCT GACAGAGAAT 
GACGAAACTC CAGCTGATTG TGCAATCGCT GTTGTTAACA AAGATTTGTC GGTGTACCTT 
AAGCTTCAAG GAGCCATAAA TGCAGAGGCT GAGCGTGAAA AGCTAAGGAA GAAGAGAGAT 
GGAATCCAAA AGCTACACCA TGCTGTGACA CACATGATGG ATGCTTCTGG TTATCGCGAG 
AAGGCACCTC AAAGTGTCCA GGAAGGGGAT ATGAGAAAGC ACACTGCTTT GTTGCGGGAA 
CTGGAGGGCA TAAGTGAAGC TGAGAAGAAA CTAGATGCCA AAACCGATAA TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332758 ( XM_015777288.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632774.1 Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOa30946
Clone ID Related Accession (Same CDS sequence)	XM_015777288.2 , XM_015777288.1
Accession Version	XM_015777288.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	valine--tRNA ligase, mitochondrial 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGC AGCCCGAGGT GCATGGTCCG GAGAAGGCTC CGGAGAAGCA ACTTGATGAG 
AAAACGTTAG AACGGAAACT GAGGAAAAAT CAGAAGGCTA AAGAGAAAGA AGAGAAAAGA 
CTTAAAGCAA AACAAAAGGA GGCAGCTATG CTTCAGGCAC AGCCAGCTTT AGATGTACTC 
AAGAAGGTTG AGAAGAAACA TAGGGGAAAA GCTGTGGAGG ATGAAAATCC TGAAGATTTT 
ATTGATCAAG ACACTCCTAA TGGGCAGAAG AAACTGCTTG CACCTCAAAT GGCCAATCAG 
TATTGCCCAA GCACGGTTGA GAAATCATGG TATGCCTGGT GGGAATCATC AGGATATTTT 
AGGGCAGATT CTGCAAGCAC AAAGCCACCA TTTGTTATAG TTATGCCACC TCCAAATGTG 
ACTGGAGCTC TTCACATAGG CCATGCTCTT ACAGTGGCTA TAGAGGATGC GATGATACGA 
TGGCGGAGGA TGTCAGGTTA TAATGCTCTA TGGGTCCCTG GAGTGGACCA TGCTGGCATT 
GCTACACAGG TTGTAGTAGA AAAAAAGCTT ATGCGTGAAA GGAATCTGAC AAGGCATGAC 
ATAGGGCCTG ACAATTTTGT TTGTGAAGTT CTCAAATGGA AAGAGCGGTA TGGTGGTACT 
ATATTGAATC AATTGCACCG CCTTGGGGCC TCACTTGATT GGTCTCGTGA GGCTTTTACA 
ATGGATGAGC AAAGGTCAAA TGCTGTAACT GAAGCTTTTG TAAGGTTACA CAAGGAAGGC 
CTGATATATA GAGATAACCG CCTTGTGAAT TGGGACTGCA CACTTCTGAC TTCAATTTCT 
GAGATAGAGG TTGATCATAT TGATCTTAAA GAAGAAACAA TGTTAAAGAT CCCTGGTTAT 
GCTACTCCTG TACAATTTGG TGTGTTGATA TCTTTTGCCT ATCCTCTTGA AGAAGGGCTA 
GGTGAGATTG TTGTTGCAAC AACTCGAATT GAAACAATGC TTGGTGATAC AGCAATAGCT 
GTTCATCCTG AGGATAAAAG GTATATGCAT CTGCATGGAC GGTATGCAGT CCACCCCTTC 
AATGGCCGAA AACTCAAAAT AATCTGTGAT GCTGAGATAG TTGATCCCAG TTTCGGTACT 
GGGGCTGTCA AGATTGCACC TGCACATGAC CCAAATGACT TTGAGGTTGG AAGACGGAAT 
AACCTTCAGT TCATTAATAT CTTGACAGAT GATGGGAAAA TAAACAGCAA TGGGGGTGCA 
CAATTTGAAG GAATGCCAAG ATTCACTGCT CGAATTTGCA TTATTGAAGC ACTGAAGGCA 
AAGGGCTTGT ACAAGGGTGC AAAGAATACC GAAACGAGTT TGGGCATTTG TTCAAGAACT 
AACGACATAG TGGAGCCTAT GATAAAATCC CCGCAGTGGT TTGTTAATTG TAACACCATG 
GCCAAGGTAG CCCTTGATGC TGTAAGGTCT AAGAGGATTG AGATCATTCC CCCGCAGTAC 
GAACAAGACT GGTATAGATG GCTTGAAAAT ATACGTGATT GGTGTGTTTC AAGGCAACAT 
TGGTGGGGAC ACCGTGTACC TGCATGGTAT GTGACACTAG AAGATGACCA GGAGAAAACT 
CTAGGTTCTG ACAATGACCG CTGGATAGTC GCCAAAAGTG AAAGTGCTGC AAGCGTTGAG 
GCACAGAAAA GTTATCCAGG AAAGAAATTC ATCCTAAATC AGGATCCTGA TGTGCTAGAC 
ACATGGTTTT CATCTGCTCT CTTCCCCTTA GCAGTACTTG GTTGGCCAGA TGATACTGCT 
GACCTCCGTT CTTTCTACCC TACTTCAGTT CTAGAAACTG GACTTGATAT TCTCTTCTGT 
TGGGTAGCAC GGATGGTGAT GATGGGAACA CAACTTGGTG GTGATGTGCC GTTTCAGAAG 
GTTTATTTGC ATCCTATTGT ATGCGACACA CATGGCCGAA AAATGTGCAA GTCACTTGGT 
AATGTCATTG ATCCTCTTGA GGTGATAAAT GGCATGACAC TCGAGGGTCT TGTAAAACGT 
CTGGAAGAAG GTAATCTGGA TCCAGATGAA TTAAACCTTG AACGGAAGCT GACAGATTAT 
CCTGATGGAA TTGCTGAATG TGGTACTGAT GCCCTCCGCT TTGCACTGAT TTCTTACACT 
TCTCAGTCTG ACAGGATAAA TCTTGATATC AAGAGAGTGG TTGGATACCG ACAATGGTGC 
AATAAATTGT GGAATGCCAT CCGTTTTGCC ATGGGCAAGC TTGGTGTTCA CTACAGTCCA 
CCTGCAACTG TCGACGTGTC TATCATGCCA CCAATCTGCA AGTGGATACT CTCAGCACTA 
AACAAGGCTA CCGGCAAAAC TGTTACCTCT TTAGAAGCAT ACAAATTCTC TGATGCAACA 
TCTGCTATAT ACTCTTGGTG GCAATACCAG CTATGTGATG TATTTATAGA AGCTATAAAA 
CCTTACTTCT TCAACGAACC TCAAGAGTTT GAATCTGCAA GAGTCGCCTC CAGAGATACT 
CTATGGGTTT GCCTGGAGAC TGGTTTGCGC TTGCTCCACC CATTCATGCC TTACATAACA 
GAAGAGCTAT GGCAGCATCT TCCACAACCC AAATATTCTT GTAGGCAAGA TTCAATTATG 
ATTTCGGAAT ATCCATCTCT CGTTGAGGAA TGGACTAATG ACAATCTTGA AAATGAAATG 
GACATTGTCT TAGACACCGT GAACAAAATA AGGTCTTTGA AGACACGAAC AGAAAGAAAA 
GAAAGACGTC CTGCTTTTGC ACTCTGCCGA AGCCAAGATG TCACTGCCAC TATTCAATGC 
CATCAATCTC TAATTGTGTG TCTTTCTTCC GTATCATTTC TTAAGATCCT GACAGAGAAT 
GACGAAACTC CAGCTGATTG TGCAATCGCT GTTGTTAACA AAGATTTGTC GGTGTACCTT 
AAGCTTCAAG GAGCCATAAA TGCAGAGGCT GAGCGTGAAA AGCTAAGGAA GAAGAGAGAT 
GGAATCCAAA AGCTACACCA TGCTGTGACA CACATGATGG ATGCTTCTGG TTATCGCGAG 
AAGGCACCTC AAAGTGTCCA GGAAGGGGAT ATGAGAAAGC ACACTGCTTT GTTGCGGGAA 
CTGGAGGGCA TAAGTGAAGC TGAGAAGAAA CTAGATGCCA AAACCGATAA TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632774.1
CDS	430..3606
Translation	MEKQPEVHGPEKAPEKQLDEKTLERKLRKNQKAKEKEEKRLKAKQKEAAMLQAQPALDVLKKVEKKHRGKAVEDENPEDFIDQDTPNGQKKLLAPQMANQYCPSTVEKSWYAWWESSGYFRADSASTKPPFVIVMPPPNVTGALHIGHALTVAIEDAMIRWRRMSGYNALWVPGVDHAGIATQVVVEKKLMRERNLTRHDIGPDNFVCEVLKWKERYGGTILNQLHRLGASLDWSREAFTMDEQRSNAVTEAFVRLHKEGLIYRDNRLVNWDCTLLTSISEIEVDHIDLKEETMLKIPGYATPVQFGVLISFAYPLEEGLGEIVVATTRIETMLGDTAIAVHPEDKRYMHLHGRYAVHPFNGRKLKIICDAEIVDPSFGTGAVKIAPAHDPNDFEVGRRNNLQFINILTDDGKINSNGGAQFEGMPRFTARICIIEALKAKGLYKGAKNTETSLGICSRTNDIVEPMIKSPQWFVNCNTMAKVALDAVRSKRIEIIPPQYEQDWYRWLENIRDWCVSRQHWWGHRVPAWYVTLEDDQEKTLGSDNDRWIVAKSESAASVEAQKSYPGKKFILNQDPDVLDTWFSSALFPLAVLGWPDDTADLRSFYPTSVLETGLDILFCWVARMVMMGTQLGGDVPFQKVYLHPIVCDTHGRKMCKSLGNVIDPLEVINGMTLEGLVKRLEEGNLDPDELNLERKLTDYPDGIAECGTDALRFALISYTSQSDRINLDIKRVVGYRQWCNKLWNAIRFAMGKLGVHYSPPATVDVSIMPPICKWILSALNKATGKTVTSLEAYKFSDATSAIYSWWQYQLCDVFIEAIKPYFFNEPQEFESARVASRDTLWVCLETGLRLLHPFMPYITEELWQHLPQPKYSCRQDSIMISEYPSLVEEWTNDNLENEMDIVLDTVNKIRSLKTRTERKERRPAFALCRSQDVTATIQCHQSLIVCLSSVSFLKILTENDETPADCAIAVVNKDLSVYLKLQGAINAEAEREKLRKKRDGIQKLHHAVTHMMDASGYREKAPQSVQEGDMRKHTALLRELEGISEAEKKLDAKTDNI

Target ORF information:

RefSeq Version	XM_015777288.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777288.1

ORF Insert Sequence:

ATGGAAAAGC AGCCCGAGGT GCATGGTCCG GAGAAGGCTC CGGAGAAGCA ACTTGATGAG 
AAAACGTTAG AACGGAAACT GAGGAAAAAT CAGAAGGCTA AAGAGAAAGA AGAGAAAAGA 
CTTAAAGCAA AACAAAAGGA GGCAGCTATG CTTCAGGCAC AGCCAGCTTT AGATGTACTC 
AAGAAGGTTG AGAAGAAACA TAGGGGAAAA GCTGTGGAGG ATGAAAATCC TGAAGATTTT 
ATTGATCAAG ACACTCCTAA TGGGCAGAAG AAACTGCTTG CACCTCAAAT GGCCAATCAG 
TATTGCCCAA GCACGGTTGA GAAATCATGG TATGCCTGGT GGGAATCATC AGGATATTTT 
AGGGCAGATT CTGCAAGCAC AAAGCCACCA TTTGTTATAG TTATGCCACC TCCAAATGTG 
ACTGGAGCTC TTCACATAGG CCATGCTCTT ACAGTGGCTA TAGAGGATGC GATGATACGA 
TGGCGGAGGA TGTCAGGTTA TAATGCTCTA TGGGTCCCTG GAGTGGACCA TGCTGGCATT 
GCTACACAGG TTGTAGTAGA AAAAAAGCTT ATGCGTGAAA GGAATCTGAC AAGGCATGAC 
ATAGGGCCTG ACAATTTTGT TTGTGAAGTT CTCAAATGGA AAGAGCGGTA TGGTGGTACT 
ATATTGAATC AATTGCACCG CCTTGGGGCC TCACTTGATT GGTCTCGTGA GGCTTTTACA 
ATGGATGAGC AAAGGTCAAA TGCTGTAACT GAAGCTTTTG TAAGGTTACA CAAGGAAGGC 
CTGATATATA GAGATAACCG CCTTGTGAAT TGGGACTGCA CACTTCTGAC TTCAATTTCT 
GAGATAGAGG TTGATCATAT TGATCTTAAA GAAGAAACAA TGTTAAAGAT CCCTGGTTAT 
GCTACTCCTG TACAATTTGG TGTGTTGATA TCTTTTGCCT ATCCTCTTGA AGAAGGGCTA 
GGTGAGATTG TTGTTGCAAC AACTCGAATT GAAACAATGC TTGGTGATAC AGCAATAGCT 
GTTCATCCTG AGGATAAAAG GTATATGCAT CTGCATGGAC GGTATGCAGT CCACCCCTTC 
AATGGCCGAA AACTCAAAAT AATCTGTGAT GCTGAGATAG TTGATCCCAG TTTCGGTACT 
GGGGCTGTCA AGATTGCACC TGCACATGAC CCAAATGACT TTGAGGTTGG AAGACGGAAT 
AACCTTCAGT TCATTAATAT CTTGACAGAT GATGGGAAAA TAAACAGCAA TGGGGGTGCA 
CAATTTGAAG GAATGCCAAG ATTCACTGCT CGAATTTGCA TTATTGAAGC ACTGAAGGCA 
AAGGGCTTGT ACAAGGGTGC AAAGAATACC GAAACGAGTT TGGGCATTTG TTCAAGAACT 
AACGACATAG TGGAGCCTAT GATAAAATCC CCGCAGTGGT TTGTTAATTG TAACACCATG 
GCCAAGGTAG CCCTTGATGC TGTAAGGTCT AAGAGGATTG AGATCATTCC CCCGCAGTAC 
GAACAAGACT GGTATAGATG GCTTGAAAAT ATACGTGATT GGTGTGTTTC AAGGCAACAT 
TGGTGGGGAC ACCGTGTACC TGCATGGTAT GTGACACTAG AAGATGACCA GGAGAAAACT 
CTAGGTTCTG ACAATGACCG CTGGATAGTC GCCAAAAGTG AAAGTGCTGC AAGCGTTGAG 
GCACAGAAAA GTTATCCAGG AAAGAAATTC ATCCTAAATC AGGATCCTGA TGTGCTAGAC 
ACATGGTTTT CATCTGCTCT CTTCCCCTTA GCAGTACTTG GTTGGCCAGA TGATACTGCT 
GACCTCCGTT CTTTCTACCC TACTTCAGTT CTAGAAACTG GACTTGATAT TCTCTTCTGT 
TGGGTAGCAC GGATGGTGAT GATGGGAACA CAACTTGGTG GTGATGTGCC GTTTCAGAAG 
GTTTATTTGC ATCCTATTGT ATGCGACACA CATGGCCGAA AAATGTGCAA GTCACTTGGT 
AATGTCATTG ATCCTCTTGA GGTGATAAAT GGCATGACAC TCGAGGGTCT TGTAAAACGT 
CTGGAAGAAG GTAATCTGGA TCCAGATGAA TTAAACCTTG AACGGAAGCT GACAGATTAT 
CCTGATGGAA TTGCTGAATG TGGTACTGAT GCCCTCCGCT TTGCACTGAT TTCTTACACT 
TCTCAGTCTG ACAGGATAAA TCTTGATATC AAGAGAGTGG TTGGATACCG ACAATGGTGC 
AATAAATTGT GGAATGCCAT CCGTTTTGCC ATGGGCAAGC TTGGTGTTCA CTACAGTCCA 
CCTGCAACTG TCGACGTGTC TATCATGCCA CCAATCTGCA AGTGGATACT CTCAGCACTA 
AACAAGGCTA CCGGCAAAAC TGTTACCTCT TTAGAAGCAT ACAAATTCTC TGATGCAACA 
TCTGCTATAT ACTCTTGGTG GCAATACCAG CTATGTGATG TATTTATAGA AGCTATAAAA 
CCTTACTTCT TCAACGAACC TCAAGAGTTT GAATCTGCAA GAGTCGCCTC CAGAGATACT 
CTATGGGTTT GCCTGGAGAC TGGTTTGCGC TTGCTCCACC CATTCATGCC TTACATAACA 
GAAGAGCTAT GGCAGCATCT TCCACAACCC AAATATTCTT GTAGGCAAGA TTCAATTATG 
ATTTCGGAAT ATCCATCTCT CGTTGAGGAA TGGACTAATG ACAATCTTGA AAATGAAATG 
GACATTGTCT TAGACACCGT GAACAAAATA AGGTCTTTGA AGACACGAAC AGAAAGAAAA 
GAAAGACGTC CTGCTTTTGC ACTCTGCCGA AGCCAAGATG TCACTGCCAC TATTCAATGC 
CATCAATCTC TAATTGTGTG TCTTTCTTCC GTATCATTTC TTAAGATCCT GACAGAGAAT 
GACGAAACTC CAGCTGATTG TGCAATCGCT GTTGTTAACA AAGATTTGTC GGTGTACCTT 
AAGCTTCAAG GAGCCATAAA TGCAGAGGCT GAGCGTGAAA AGCTAAGGAA GAAGAGAGAT 
GGAATCCAAA AGCTACACCA TGCTGTGACA CACATGATGG ATGCTTCTGG TTATCGCGAG 
AAGGCACCTC AAAGTGTCCA GGAAGGGGAT ATGAGAAAGC ACACTGCTTT GTTGCGGGAA 
CTGGAGGGCA TAAGTGAAGC TGAGAAGAAA CTAGATGCCA AAACCGATAA TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332758 ( XM_015777289.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632775.1 Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOa30947
Clone ID Related Accession (Same CDS sequence)	XM_015777289.2 , XM_015777289.1
Accession Version	XM_015777289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	valine--tRNA ligase, mitochondrial 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015777289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGC CCGAGGTGCA TGGTCCGGAG AAGGCTCCGG AGAAGCAACT TGATGAGAAA 
ACGTTAGAAC GGAAACTGAG GAAAAATCAG AAGGCTAAAG AGAAAGAAGA GAAAAGACTT 
AAAGCAAAAC AAAAGGAGGC AGCTATGCTT CAGGCACAGC CAGCTTTAGA TGTACTCAAG 
AAGGTTGAGA AGAAACATAG GGGAAAAGCT GTGGAGGATG AAAATCCTGA AGATTTTATT 
GATCAAGACA CTCCTAATGG GCAGAAGAAA CTGCTTGCAC CTCAAATGGC CAATCAGTAT 
TGCCCAAGCA CGGTTGAGAA ATCATGGTAT GCCTGGTGGG AATCATCAGG ATATTTTAGG 
GCAGATTCTG CAAGCACAAA GCCACCATTT GTTATAGTTA TGCCACCTCC AAATGTGACT 
GGAGCTCTTC ACATAGGCCA TGCTCTTACA GTGGCTATAG AGGATGCGAT GATACGATGG 
CGGAGGATGT CAGGTTATAA TGCTCTATGG GTCCCTGGAG TGGACCATGC TGGCATTGCT 
ACACAGGTTG TAGTAGAAAA AAAGCTTATG CGTGAAAGGA ATCTGACAAG GCATGACATA 
GGGCCTGACA ATTTTGTTTG TGAAGTTCTC AAATGGAAAG AGCGGTATGG TGGTACTATA 
TTGAATCAAT TGCACCGCCT TGGGGCCTCA CTTGATTGGT CTCGTGAGGC TTTTACAATG 
GATGAGCAAA GGTCAAATGC TGTAACTGAA GCTTTTGTAA GGTTACACAA GGAAGGCCTG 
ATATATAGAG ATAACCGCCT TGTGAATTGG GACTGCACAC TTCTGACTTC AATTTCTGAG 
ATAGAGGTTG ATCATATTGA TCTTAAAGAA GAAACAATGT TAAAGATCCC TGGTTATGCT 
ACTCCTGTAC AATTTGGTGT GTTGATATCT TTTGCCTATC CTCTTGAAGA AGGGCTAGGT 
GAGATTGTTG TTGCAACAAC TCGAATTGAA ACAATGCTTG GTGATACAGC AATAGCTGTT 
CATCCTGAGG ATAAAAGGTA TATGCATCTG CATGGACGGT ATGCAGTCCA CCCCTTCAAT 
GGCCGAAAAC TCAAAATAAT CTGTGATGCT GAGATAGTTG ATCCCAGTTT CGGTACTGGG 
GCTGTCAAGA TTGCACCTGC ACATGACCCA AATGACTTTG AGGTTGGAAG ACGGAATAAC 
CTTCAGTTCA TTAATATCTT GACAGATGAT GGGAAAATAA ACAGCAATGG GGGTGCACAA 
TTTGAAGGAA TGCCAAGATT CACTGCTCGA ATTTGCATTA TTGAAGCACT GAAGGCAAAG 
GGCTTGTACA AGGGTGCAAA GAATACCGAA ACGAGTTTGG GCATTTGTTC AAGAACTAAC 
GACATAGTGG AGCCTATGAT AAAATCCCCG CAGTGGTTTG TTAATTGTAA CACCATGGCC 
AAGGTAGCCC TTGATGCTGT AAGGTCTAAG AGGATTGAGA TCATTCCCCC GCAGTACGAA 
CAAGACTGGT ATAGATGGCT TGAAAATATA CGTGATTGGT GTGTTTCAAG GCAACATTGG 
TGGGGACACC GTGTACCTGC ATGGTATGTG ACACTAGAAG ATGACCAGGA GAAAACTCTA 
GGTTCTGACA ATGACCGCTG GATAGTCGCC AAAAGTGAAA GTGCTGCAAG CGTTGAGGCA 
CAGAAAAGTT ATCCAGGAAA GAAATTCATC CTAAATCAGG ATCCTGATGT GCTAGACACA 
TGGTTTTCAT CTGCTCTCTT CCCCTTAGCA GTACTTGGTT GGCCAGATGA TACTGCTGAC 
CTCCGTTCTT TCTACCCTAC TTCAGTTCTA GAAACTGGAC TTGATATTCT CTTCTGTTGG 
GTAGCACGGA TGGTGATGAT GGGAACACAA CTTGGTGGTG ATGTGCCGTT TCAGAAGGTT 
TATTTGCATC CTATTGTATG CGACACACAT GGCCGAAAAA TGTGCAAGTC ACTTGGTAAT 
GTCATTGATC CTCTTGAGGT GATAAATGGC ATGACACTCG AGGGTCTTGT AAAACGTCTG 
GAAGAAGGTA ATCTGGATCC AGATGAATTA AACCTTGAAC GGAAGCTGAC AGATTATCCT 
GATGGAATTG CTGAATGTGG TACTGATGCC CTCCGCTTTG CACTGATTTC TTACACTTCT 
CAGTCTGACA GGATAAATCT TGATATCAAG AGAGTGGTTG GATACCGACA ATGGTGCAAT 
AAATTGTGGA ATGCCATCCG TTTTGCCATG GGCAAGCTTG GTGTTCACTA CAGTCCACCT 
GCAACTGTCG ACGTGTCTAT CATGCCACCA ATCTGCAAGT GGATACTCTC AGCACTAAAC 
AAGGCTACCG GCAAAACTGT TACCTCTTTA GAAGCATACA AATTCTCTGA TGCAACATCT 
GCTATATACT CTTGGTGGCA ATACCAGCTA TGTGATGTAT TTATAGAAGC TATAAAACCT 
TACTTCTTCA ACGAACCTCA AGAGTTTGAA TCTGCAAGAG TCGCCTCCAG AGATACTCTA 
TGGGTTTGCC TGGAGACTGG TTTGCGCTTG CTCCACCCAT TCATGCCTTA CATAACAGAA 
GAGCTATGGC AGCATCTTCC ACAACCCAAA TATTCTTGTA GGCAAGATTC AATTATGATT 
TCGGAATATC CATCTCTCGT TGAGGAATGG ACTAATGACA ATCTTGAAAA TGAAATGGAC 
ATTGTCTTAG ACACCGTGAA CAAAATAAGG TCTTTGAAGA CACGAACAGA AAGAAAAGAA 
AGACGTCCTG CTTTTGCACT CTGCCGAAGC CAAGATGTCA CTGCCACTAT TCAATGCCAT 
CAATCTCTAA TTGTGTGTCT TTCTTCCGTA TCATTTCTTA AGATCCTGAC AGAGAATGAC 
GAAACTCCAG CTGATTGTGC AATCGCTGTT GTTAACAAAG ATTTGTCGGT GTACCTTAAG 
CTTCAAGGAG CCATAAATGC AGAGGCTGAG CGTGAAAAGC TAAGGAAGAA GAGAGATGGA 
ATCCAAAAGC TACACCATGC TGTGACACAC ATGATGGATG CTTCTGGTTA TCGCGAGAAG 
GCACCTCAAA GTGTCCAGGA AGGGGATATG AGAAAGCACA CTGCTTTGTT GCGGGAACTG 
GAGGGCATAA GTGAAGCTGA GAAGAAACTA GATGCCAAAA CCGATAATAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632775.1
CDS	434..3607
Translation	MEKPEVHGPEKAPEKQLDEKTLERKLRKNQKAKEKEEKRLKAKQKEAAMLQAQPALDVLKKVEKKHRGKAVEDENPEDFIDQDTPNGQKKLLAPQMANQYCPSTVEKSWYAWWESSGYFRADSASTKPPFVIVMPPPNVTGALHIGHALTVAIEDAMIRWRRMSGYNALWVPGVDHAGIATQVVVEKKLMRERNLTRHDIGPDNFVCEVLKWKERYGGTILNQLHRLGASLDWSREAFTMDEQRSNAVTEAFVRLHKEGLIYRDNRLVNWDCTLLTSISEIEVDHIDLKEETMLKIPGYATPVQFGVLISFAYPLEEGLGEIVVATTRIETMLGDTAIAVHPEDKRYMHLHGRYAVHPFNGRKLKIICDAEIVDPSFGTGAVKIAPAHDPNDFEVGRRNNLQFINILTDDGKINSNGGAQFEGMPRFTARICIIEALKAKGLYKGAKNTETSLGICSRTNDIVEPMIKSPQWFVNCNTMAKVALDAVRSKRIEIIPPQYEQDWYRWLENIRDWCVSRQHWWGHRVPAWYVTLEDDQEKTLGSDNDRWIVAKSESAASVEAQKSYPGKKFILNQDPDVLDTWFSSALFPLAVLGWPDDTADLRSFYPTSVLETGLDILFCWVARMVMMGTQLGGDVPFQKVYLHPIVCDTHGRKMCKSLGNVIDPLEVINGMTLEGLVKRLEEGNLDPDELNLERKLTDYPDGIAECGTDALRFALISYTSQSDRINLDIKRVVGYRQWCNKLWNAIRFAMGKLGVHYSPPATVDVSIMPPICKWILSALNKATGKTVTSLEAYKFSDATSAIYSWWQYQLCDVFIEAIKPYFFNEPQEFESARVASRDTLWVCLETGLRLLHPFMPYITEELWQHLPQPKYSCRQDSIMISEYPSLVEEWTNDNLENEMDIVLDTVNKIRSLKTRTERKERRPAFALCRSQDVTATIQCHQSLIVCLSSVSFLKILTENDETPADCAIAVVNKDLSVYLKLQGAINAEAEREKLRKKRDGIQKLHHAVTHMMDASGYREKAPQSVQEGDMRKHTALLRELEGISEAEKKLDAKTDNI

Target ORF information:

RefSeq Version	XM_015777289.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777289.2

ORF Insert Sequence:

ATGGAAAAGC CCGAGGTGCA TGGTCCGGAG AAGGCTCCGG AGAAGCAACT TGATGAGAAA 
ACGTTAGAAC GGAAACTGAG GAAAAATCAG AAGGCTAAAG AGAAAGAAGA GAAAAGACTT 
AAAGCAAAAC AAAAGGAGGC AGCTATGCTT CAGGCACAGC CAGCTTTAGA TGTACTCAAG 
AAGGTTGAGA AGAAACATAG GGGAAAAGCT GTGGAGGATG AAAATCCTGA AGATTTTATT 
GATCAAGACA CTCCTAATGG GCAGAAGAAA CTGCTTGCAC CTCAAATGGC CAATCAGTAT 
TGCCCAAGCA CGGTTGAGAA ATCATGGTAT GCCTGGTGGG AATCATCAGG ATATTTTAGG 
GCAGATTCTG CAAGCACAAA GCCACCATTT GTTATAGTTA TGCCACCTCC AAATGTGACT 
GGAGCTCTTC ACATAGGCCA TGCTCTTACA GTGGCTATAG AGGATGCGAT GATACGATGG 
CGGAGGATGT CAGGTTATAA TGCTCTATGG GTCCCTGGAG TGGACCATGC TGGCATTGCT 
ACACAGGTTG TAGTAGAAAA AAAGCTTATG CGTGAAAGGA ATCTGACAAG GCATGACATA 
GGGCCTGACA ATTTTGTTTG TGAAGTTCTC AAATGGAAAG AGCGGTATGG TGGTACTATA 
TTGAATCAAT TGCACCGCCT TGGGGCCTCA CTTGATTGGT CTCGTGAGGC TTTTACAATG 
GATGAGCAAA GGTCAAATGC TGTAACTGAA GCTTTTGTAA GGTTACACAA GGAAGGCCTG 
ATATATAGAG ATAACCGCCT TGTGAATTGG GACTGCACAC TTCTGACTTC AATTTCTGAG 
ATAGAGGTTG ATCATATTGA TCTTAAAGAA GAAACAATGT TAAAGATCCC TGGTTATGCT 
ACTCCTGTAC AATTTGGTGT GTTGATATCT TTTGCCTATC CTCTTGAAGA AGGGCTAGGT 
GAGATTGTTG TTGCAACAAC TCGAATTGAA ACAATGCTTG GTGATACAGC AATAGCTGTT 
CATCCTGAGG ATAAAAGGTA TATGCATCTG CATGGACGGT ATGCAGTCCA CCCCTTCAAT 
GGCCGAAAAC TCAAAATAAT CTGTGATGCT GAGATAGTTG ATCCCAGTTT CGGTACTGGG 
GCTGTCAAGA TTGCACCTGC ACATGACCCA AATGACTTTG AGGTTGGAAG ACGGAATAAC 
CTTCAGTTCA TTAATATCTT GACAGATGAT GGGAAAATAA ACAGCAATGG GGGTGCACAA 
TTTGAAGGAA TGCCAAGATT CACTGCTCGA ATTTGCATTA TTGAAGCACT GAAGGCAAAG 
GGCTTGTACA AGGGTGCAAA GAATACCGAA ACGAGTTTGG GCATTTGTTC AAGAACTAAC 
GACATAGTGG AGCCTATGAT AAAATCCCCG CAGTGGTTTG TTAATTGTAA CACCATGGCC 
AAGGTAGCCC TTGATGCTGT AAGGTCTAAG AGGATTGAGA TCATTCCCCC GCAGTACGAA 
CAAGACTGGT ATAGATGGCT TGAAAATATA CGTGATTGGT GTGTTTCAAG GCAACATTGG 
TGGGGACACC GTGTACCTGC ATGGTATGTG ACACTAGAAG ATGACCAGGA GAAAACTCTA 
GGTTCTGACA ATGACCGCTG GATAGTCGCC AAAAGTGAAA GTGCTGCAAG CGTTGAGGCA 
CAGAAAAGTT ATCCAGGAAA GAAATTCATC CTAAATCAGG ATCCTGATGT GCTAGACACA 
TGGTTTTCAT CTGCTCTCTT CCCCTTAGCA GTACTTGGTT GGCCAGATGA TACTGCTGAC 
CTCCGTTCTT TCTACCCTAC TTCAGTTCTA GAAACTGGAC TTGATATTCT CTTCTGTTGG 
GTAGCACGGA TGGTGATGAT GGGAACACAA CTTGGTGGTG ATGTGCCGTT TCAGAAGGTT 
TATTTGCATC CTATTGTATG CGACACACAT GGCCGAAAAA TGTGCAAGTC ACTTGGTAAT 
GTCATTGATC CTCTTGAGGT GATAAATGGC ATGACACTCG AGGGTCTTGT AAAACGTCTG 
GAAGAAGGTA ATCTGGATCC AGATGAATTA AACCTTGAAC GGAAGCTGAC AGATTATCCT 
GATGGAATTG CTGAATGTGG TACTGATGCC CTCCGCTTTG CACTGATTTC TTACACTTCT 
CAGTCTGACA GGATAAATCT TGATATCAAG AGAGTGGTTG GATACCGACA ATGGTGCAAT 
AAATTGTGGA ATGCCATCCG TTTTGCCATG GGCAAGCTTG GTGTTCACTA CAGTCCACCT 
GCAACTGTCG ACGTGTCTAT CATGCCACCA ATCTGCAAGT GGATACTCTC AGCACTAAAC 
AAGGCTACCG GCAAAACTGT TACCTCTTTA GAAGCATACA AATTCTCTGA TGCAACATCT 
GCTATATACT CTTGGTGGCA ATACCAGCTA TGTGATGTAT TTATAGAAGC TATAAAACCT 
TACTTCTTCA ACGAACCTCA AGAGTTTGAA TCTGCAAGAG TCGCCTCCAG AGATACTCTA 
TGGGTTTGCC TGGAGACTGG TTTGCGCTTG CTCCACCCAT TCATGCCTTA CATAACAGAA 
GAGCTATGGC AGCATCTTCC ACAACCCAAA TATTCTTGTA GGCAAGATTC AATTATGATT 
TCGGAATATC CATCTCTCGT TGAGGAATGG ACTAATGACA ATCTTGAAAA TGAAATGGAC 
ATTGTCTTAG ACACCGTGAA CAAAATAAGG TCTTTGAAGA CACGAACAGA AAGAAAAGAA 
AGACGTCCTG CTTTTGCACT CTGCCGAAGC CAAGATGTCA CTGCCACTAT TCAATGCCAT 
CAATCTCTAA TTGTGTGTCT TTCTTCCGTA TCATTTCTTA AGATCCTGAC AGAGAATGAC 
GAAACTCCAG CTGATTGTGC AATCGCTGTT GTTAACAAAG ATTTGTCGGT GTACCTTAAG 
CTTCAAGGAG CCATAAATGC AGAGGCTGAG CGTGAAAAGC TAAGGAAGAA GAGAGATGGA 
ATCCAAAAGC TACACCATGC TGTGACACAC ATGATGGATG CTTCTGGTTA TCGCGAGAAG 
GCACCTCAAA GTGTCCAGGA AGGGGATATG AGAAAGCACA CTGCTTTGTT GCGGGAACTG 
GAGGGCATAA GTGAAGCTGA GAAGAAACTA GATGCCAAAA CCGATAATAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332758 ( XM_015777289.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632775.1 Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOa30947
Clone ID Related Accession (Same CDS sequence)	XM_015777289.2 , XM_015777289.1
Accession Version	XM_015777289.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	valine--tRNA ligase, mitochondrial 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGC CCGAGGTGCA TGGTCCGGAG AAGGCTCCGG AGAAGCAACT TGATGAGAAA 
ACGTTAGAAC GGAAACTGAG GAAAAATCAG AAGGCTAAAG AGAAAGAAGA GAAAAGACTT 
AAAGCAAAAC AAAAGGAGGC AGCTATGCTT CAGGCACAGC CAGCTTTAGA TGTACTCAAG 
AAGGTTGAGA AGAAACATAG GGGAAAAGCT GTGGAGGATG AAAATCCTGA AGATTTTATT 
GATCAAGACA CTCCTAATGG GCAGAAGAAA CTGCTTGCAC CTCAAATGGC CAATCAGTAT 
TGCCCAAGCA CGGTTGAGAA ATCATGGTAT GCCTGGTGGG AATCATCAGG ATATTTTAGG 
GCAGATTCTG CAAGCACAAA GCCACCATTT GTTATAGTTA TGCCACCTCC AAATGTGACT 
GGAGCTCTTC ACATAGGCCA TGCTCTTACA GTGGCTATAG AGGATGCGAT GATACGATGG 
CGGAGGATGT CAGGTTATAA TGCTCTATGG GTCCCTGGAG TGGACCATGC TGGCATTGCT 
ACACAGGTTG TAGTAGAAAA AAAGCTTATG CGTGAAAGGA ATCTGACAAG GCATGACATA 
GGGCCTGACA ATTTTGTTTG TGAAGTTCTC AAATGGAAAG AGCGGTATGG TGGTACTATA 
TTGAATCAAT TGCACCGCCT TGGGGCCTCA CTTGATTGGT CTCGTGAGGC TTTTACAATG 
GATGAGCAAA GGTCAAATGC TGTAACTGAA GCTTTTGTAA GGTTACACAA GGAAGGCCTG 
ATATATAGAG ATAACCGCCT TGTGAATTGG GACTGCACAC TTCTGACTTC AATTTCTGAG 
ATAGAGGTTG ATCATATTGA TCTTAAAGAA GAAACAATGT TAAAGATCCC TGGTTATGCT 
ACTCCTGTAC AATTTGGTGT GTTGATATCT TTTGCCTATC CTCTTGAAGA AGGGCTAGGT 
GAGATTGTTG TTGCAACAAC TCGAATTGAA ACAATGCTTG GTGATACAGC AATAGCTGTT 
CATCCTGAGG ATAAAAGGTA TATGCATCTG CATGGACGGT ATGCAGTCCA CCCCTTCAAT 
GGCCGAAAAC TCAAAATAAT CTGTGATGCT GAGATAGTTG ATCCCAGTTT CGGTACTGGG 
GCTGTCAAGA TTGCACCTGC ACATGACCCA AATGACTTTG AGGTTGGAAG ACGGAATAAC 
CTTCAGTTCA TTAATATCTT GACAGATGAT GGGAAAATAA ACAGCAATGG GGGTGCACAA 
TTTGAAGGAA TGCCAAGATT CACTGCTCGA ATTTGCATTA TTGAAGCACT GAAGGCAAAG 
GGCTTGTACA AGGGTGCAAA GAATACCGAA ACGAGTTTGG GCATTTGTTC AAGAACTAAC 
GACATAGTGG AGCCTATGAT AAAATCCCCG CAGTGGTTTG TTAATTGTAA CACCATGGCC 
AAGGTAGCCC TTGATGCTGT AAGGTCTAAG AGGATTGAGA TCATTCCCCC GCAGTACGAA 
CAAGACTGGT ATAGATGGCT TGAAAATATA CGTGATTGGT GTGTTTCAAG GCAACATTGG 
TGGGGACACC GTGTACCTGC ATGGTATGTG ACACTAGAAG ATGACCAGGA GAAAACTCTA 
GGTTCTGACA ATGACCGCTG GATAGTCGCC AAAAGTGAAA GTGCTGCAAG CGTTGAGGCA 
CAGAAAAGTT ATCCAGGAAA GAAATTCATC CTAAATCAGG ATCCTGATGT GCTAGACACA 
TGGTTTTCAT CTGCTCTCTT CCCCTTAGCA GTACTTGGTT GGCCAGATGA TACTGCTGAC 
CTCCGTTCTT TCTACCCTAC TTCAGTTCTA GAAACTGGAC TTGATATTCT CTTCTGTTGG 
GTAGCACGGA TGGTGATGAT GGGAACACAA CTTGGTGGTG ATGTGCCGTT TCAGAAGGTT 
TATTTGCATC CTATTGTATG CGACACACAT GGCCGAAAAA TGTGCAAGTC ACTTGGTAAT 
GTCATTGATC CTCTTGAGGT GATAAATGGC ATGACACTCG AGGGTCTTGT AAAACGTCTG 
GAAGAAGGTA ATCTGGATCC AGATGAATTA AACCTTGAAC GGAAGCTGAC AGATTATCCT 
GATGGAATTG CTGAATGTGG TACTGATGCC CTCCGCTTTG CACTGATTTC TTACACTTCT 
CAGTCTGACA GGATAAATCT TGATATCAAG AGAGTGGTTG GATACCGACA ATGGTGCAAT 
AAATTGTGGA ATGCCATCCG TTTTGCCATG GGCAAGCTTG GTGTTCACTA CAGTCCACCT 
GCAACTGTCG ACGTGTCTAT CATGCCACCA ATCTGCAAGT GGATACTCTC AGCACTAAAC 
AAGGCTACCG GCAAAACTGT TACCTCTTTA GAAGCATACA AATTCTCTGA TGCAACATCT 
GCTATATACT CTTGGTGGCA ATACCAGCTA TGTGATGTAT TTATAGAAGC TATAAAACCT 
TACTTCTTCA ACGAACCTCA AGAGTTTGAA TCTGCAAGAG TCGCCTCCAG AGATACTCTA 
TGGGTTTGCC TGGAGACTGG TTTGCGCTTG CTCCACCCAT TCATGCCTTA CATAACAGAA 
GAGCTATGGC AGCATCTTCC ACAACCCAAA TATTCTTGTA GGCAAGATTC AATTATGATT 
TCGGAATATC CATCTCTCGT TGAGGAATGG ACTAATGACA ATCTTGAAAA TGAAATGGAC 
ATTGTCTTAG ACACCGTGAA CAAAATAAGG TCTTTGAAGA CACGAACAGA AAGAAAAGAA 
AGACGTCCTG CTTTTGCACT CTGCCGAAGC CAAGATGTCA CTGCCACTAT TCAATGCCAT 
CAATCTCTAA TTGTGTGTCT TTCTTCCGTA TCATTTCTTA AGATCCTGAC AGAGAATGAC 
GAAACTCCAG CTGATTGTGC AATCGCTGTT GTTAACAAAG ATTTGTCGGT GTACCTTAAG 
CTTCAAGGAG CCATAAATGC AGAGGCTGAG CGTGAAAAGC TAAGGAAGAA GAGAGATGGA 
ATCCAAAAGC TACACCATGC TGTGACACAC ATGATGGATG CTTCTGGTTA TCGCGAGAAG 
GCACCTCAAA GTGTCCAGGA AGGGGATATG AGAAAGCACA CTGCTTTGTT GCGGGAACTG 
GAGGGCATAA GTGAAGCTGA GAAGAAACTA GATGCCAAAA CCGATAATAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632775.1
CDS	430..3603
Translation	MEKPEVHGPEKAPEKQLDEKTLERKLRKNQKAKEKEEKRLKAKQKEAAMLQAQPALDVLKKVEKKHRGKAVEDENPEDFIDQDTPNGQKKLLAPQMANQYCPSTVEKSWYAWWESSGYFRADSASTKPPFVIVMPPPNVTGALHIGHALTVAIEDAMIRWRRMSGYNALWVPGVDHAGIATQVVVEKKLMRERNLTRHDIGPDNFVCEVLKWKERYGGTILNQLHRLGASLDWSREAFTMDEQRSNAVTEAFVRLHKEGLIYRDNRLVNWDCTLLTSISEIEVDHIDLKEETMLKIPGYATPVQFGVLISFAYPLEEGLGEIVVATTRIETMLGDTAIAVHPEDKRYMHLHGRYAVHPFNGRKLKIICDAEIVDPSFGTGAVKIAPAHDPNDFEVGRRNNLQFINILTDDGKINSNGGAQFEGMPRFTARICIIEALKAKGLYKGAKNTETSLGICSRTNDIVEPMIKSPQWFVNCNTMAKVALDAVRSKRIEIIPPQYEQDWYRWLENIRDWCVSRQHWWGHRVPAWYVTLEDDQEKTLGSDNDRWIVAKSESAASVEAQKSYPGKKFILNQDPDVLDTWFSSALFPLAVLGWPDDTADLRSFYPTSVLETGLDILFCWVARMVMMGTQLGGDVPFQKVYLHPIVCDTHGRKMCKSLGNVIDPLEVINGMTLEGLVKRLEEGNLDPDELNLERKLTDYPDGIAECGTDALRFALISYTSQSDRINLDIKRVVGYRQWCNKLWNAIRFAMGKLGVHYSPPATVDVSIMPPICKWILSALNKATGKTVTSLEAYKFSDATSAIYSWWQYQLCDVFIEAIKPYFFNEPQEFESARVASRDTLWVCLETGLRLLHPFMPYITEELWQHLPQPKYSCRQDSIMISEYPSLVEEWTNDNLENEMDIVLDTVNKIRSLKTRTERKERRPAFALCRSQDVTATIQCHQSLIVCLSSVSFLKILTENDETPADCAIAVVNKDLSVYLKLQGAINAEAEREKLRKKRDGIQKLHHAVTHMMDASGYREKAPQSVQEGDMRKHTALLRELEGISEAEKKLDAKTDNI

Target ORF information:

RefSeq Version	XM_015777289.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777289.1

ORF Insert Sequence:

ATGGAAAAGC CCGAGGTGCA TGGTCCGGAG AAGGCTCCGG AGAAGCAACT TGATGAGAAA 
ACGTTAGAAC GGAAACTGAG GAAAAATCAG AAGGCTAAAG AGAAAGAAGA GAAAAGACTT 
AAAGCAAAAC AAAAGGAGGC AGCTATGCTT CAGGCACAGC CAGCTTTAGA TGTACTCAAG 
AAGGTTGAGA AGAAACATAG GGGAAAAGCT GTGGAGGATG AAAATCCTGA AGATTTTATT 
GATCAAGACA CTCCTAATGG GCAGAAGAAA CTGCTTGCAC CTCAAATGGC CAATCAGTAT 
TGCCCAAGCA CGGTTGAGAA ATCATGGTAT GCCTGGTGGG AATCATCAGG ATATTTTAGG 
GCAGATTCTG CAAGCACAAA GCCACCATTT GTTATAGTTA TGCCACCTCC AAATGTGACT 
GGAGCTCTTC ACATAGGCCA TGCTCTTACA GTGGCTATAG AGGATGCGAT GATACGATGG 
CGGAGGATGT CAGGTTATAA TGCTCTATGG GTCCCTGGAG TGGACCATGC TGGCATTGCT 
ACACAGGTTG TAGTAGAAAA AAAGCTTATG CGTGAAAGGA ATCTGACAAG GCATGACATA 
GGGCCTGACA ATTTTGTTTG TGAAGTTCTC AAATGGAAAG AGCGGTATGG TGGTACTATA 
TTGAATCAAT TGCACCGCCT TGGGGCCTCA CTTGATTGGT CTCGTGAGGC TTTTACAATG 
GATGAGCAAA GGTCAAATGC TGTAACTGAA GCTTTTGTAA GGTTACACAA GGAAGGCCTG 
ATATATAGAG ATAACCGCCT TGTGAATTGG GACTGCACAC TTCTGACTTC AATTTCTGAG 
ATAGAGGTTG ATCATATTGA TCTTAAAGAA GAAACAATGT TAAAGATCCC TGGTTATGCT 
ACTCCTGTAC AATTTGGTGT GTTGATATCT TTTGCCTATC CTCTTGAAGA AGGGCTAGGT 
GAGATTGTTG TTGCAACAAC TCGAATTGAA ACAATGCTTG GTGATACAGC AATAGCTGTT 
CATCCTGAGG ATAAAAGGTA TATGCATCTG CATGGACGGT ATGCAGTCCA CCCCTTCAAT 
GGCCGAAAAC TCAAAATAAT CTGTGATGCT GAGATAGTTG ATCCCAGTTT CGGTACTGGG 
GCTGTCAAGA TTGCACCTGC ACATGACCCA AATGACTTTG AGGTTGGAAG ACGGAATAAC 
CTTCAGTTCA TTAATATCTT GACAGATGAT GGGAAAATAA ACAGCAATGG GGGTGCACAA 
TTTGAAGGAA TGCCAAGATT CACTGCTCGA ATTTGCATTA TTGAAGCACT GAAGGCAAAG 
GGCTTGTACA AGGGTGCAAA GAATACCGAA ACGAGTTTGG GCATTTGTTC AAGAACTAAC 
GACATAGTGG AGCCTATGAT AAAATCCCCG CAGTGGTTTG TTAATTGTAA CACCATGGCC 
AAGGTAGCCC TTGATGCTGT AAGGTCTAAG AGGATTGAGA TCATTCCCCC GCAGTACGAA 
CAAGACTGGT ATAGATGGCT TGAAAATATA CGTGATTGGT GTGTTTCAAG GCAACATTGG 
TGGGGACACC GTGTACCTGC ATGGTATGTG ACACTAGAAG ATGACCAGGA GAAAACTCTA 
GGTTCTGACA ATGACCGCTG GATAGTCGCC AAAAGTGAAA GTGCTGCAAG CGTTGAGGCA 
CAGAAAAGTT ATCCAGGAAA GAAATTCATC CTAAATCAGG ATCCTGATGT GCTAGACACA 
TGGTTTTCAT CTGCTCTCTT CCCCTTAGCA GTACTTGGTT GGCCAGATGA TACTGCTGAC 
CTCCGTTCTT TCTACCCTAC TTCAGTTCTA GAAACTGGAC TTGATATTCT CTTCTGTTGG 
GTAGCACGGA TGGTGATGAT GGGAACACAA CTTGGTGGTG ATGTGCCGTT TCAGAAGGTT 
TATTTGCATC CTATTGTATG CGACACACAT GGCCGAAAAA TGTGCAAGTC ACTTGGTAAT 
GTCATTGATC CTCTTGAGGT GATAAATGGC ATGACACTCG AGGGTCTTGT AAAACGTCTG 
GAAGAAGGTA ATCTGGATCC AGATGAATTA AACCTTGAAC GGAAGCTGAC AGATTATCCT 
GATGGAATTG CTGAATGTGG TACTGATGCC CTCCGCTTTG CACTGATTTC TTACACTTCT 
CAGTCTGACA GGATAAATCT TGATATCAAG AGAGTGGTTG GATACCGACA ATGGTGCAAT 
AAATTGTGGA ATGCCATCCG TTTTGCCATG GGCAAGCTTG GTGTTCACTA CAGTCCACCT 
GCAACTGTCG ACGTGTCTAT CATGCCACCA ATCTGCAAGT GGATACTCTC AGCACTAAAC 
AAGGCTACCG GCAAAACTGT TACCTCTTTA GAAGCATACA AATTCTCTGA TGCAACATCT 
GCTATATACT CTTGGTGGCA ATACCAGCTA TGTGATGTAT TTATAGAAGC TATAAAACCT 
TACTTCTTCA ACGAACCTCA AGAGTTTGAA TCTGCAAGAG TCGCCTCCAG AGATACTCTA 
TGGGTTTGCC TGGAGACTGG TTTGCGCTTG CTCCACCCAT TCATGCCTTA CATAACAGAA 
GAGCTATGGC AGCATCTTCC ACAACCCAAA TATTCTTGTA GGCAAGATTC AATTATGATT 
TCGGAATATC CATCTCTCGT TGAGGAATGG ACTAATGACA ATCTTGAAAA TGAAATGGAC 
ATTGTCTTAG ACACCGTGAA CAAAATAAGG TCTTTGAAGA CACGAACAGA AAGAAAAGAA 
AGACGTCCTG CTTTTGCACT CTGCCGAAGC CAAGATGTCA CTGCCACTAT TCAATGCCAT 
CAATCTCTAA TTGTGTGTCT TTCTTCCGTA TCATTTCTTA AGATCCTGAC AGAGAATGAC 
GAAACTCCAG CTGATTGTGC AATCGCTGTT GTTAACAAAG ATTTGTCGGT GTACCTTAAG 
CTTCAAGGAG CCATAAATGC AGAGGCTGAG CGTGAAAAGC TAAGGAAGAA GAGAGATGGA 
ATCCAAAAGC TACACCATGC TGTGACACAC ATGATGGATG CTTCTGGTTA TCGCGAGAAG 
GCACCTCAAA GTGTCCAGGA AGGGGATATG AGAAAGCACA CTGCTTTGTT GCGGGAACTG 
GAGGGCATAA GTGAAGCTGA GAAGAAACTA GATGCCAAAA CCGATAATAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332758 ( XM_015777290.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632776.1 Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOa30948
Clone ID Related Accession (Same CDS sequence)	XM_015777290.2 , XM_026024198.1 , XM_015777290.1
Accession Version	XM_015777290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	valine--tRNA ligase, mitochondrial 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015777290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCAGG CACAGCCAGC TTTAGATGTA CTCAAGAAGG TTGAGAAGAA ACATAGGGGA 
AAAGCTGTGG AGGATGAAAA TCCTGAAGAT TTTATTGATC AAGACACTCC TAATGGGCAG 
AAGAAACTGC TTGCACCTCA AATGGCCAAT CAGTATTGCC CAAGCACGGT TGAGAAATCA 
TGGTATGCCT GGTGGGAATC ATCAGGATAT TTTAGGGCAG ATTCTGCAAG CACAAAGCCA 
CCATTTGTTA TAGTTATGCC ACCTCCAAAT GTGACTGGAG CTCTTCACAT AGGCCATGCT 
CTTACAGTGG CTATAGAGGA TGCGATGATA CGATGGCGGA GGATGTCAGG TTATAATGCT 
CTATGGGTCC CTGGAGTGGA CCATGCTGGC ATTGCTACAC AGGTTGTAGT AGAAAAAAAG 
CTTATGCGTG AAAGGAATCT GACAAGGCAT GACATAGGGC CTGACAATTT TGTTTGTGAA 
GTTCTCAAAT GGAAAGAGCG GTATGGTGGT ACTATATTGA ATCAATTGCA CCGCCTTGGG 
GCCTCACTTG ATTGGTCTCG TGAGGCTTTT ACAATGGATG AGCAAAGGTC AAATGCTGTA 
ACTGAAGCTT TTGTAAGGTT ACACAAGGAA GGCCTGATAT ATAGAGATAA CCGCCTTGTG 
AATTGGGACT GCACACTTCT GACTTCAATT TCTGAGATAG AGGTTGATCA TATTGATCTT 
AAAGAAGAAA CAATGTTAAA GATCCCTGGT TATGCTACTC CTGTACAATT TGGTGTGTTG 
ATATCTTTTG CCTATCCTCT TGAAGAAGGG CTAGGTGAGA TTGTTGTTGC AACAACTCGA 
ATTGAAACAA TGCTTGGTGA TACAGCAATA GCTGTTCATC CTGAGGATAA AAGGTATATG 
CATCTGCATG GACGGTATGC AGTCCACCCC TTCAATGGCC GAAAACTCAA AATAATCTGT 
GATGCTGAGA TAGTTGATCC CAGTTTCGGT ACTGGGGCTG TCAAGATTGC ACCTGCACAT 
GACCCAAATG ACTTTGAGGT TGGAAGACGG AATAACCTTC AGTTCATTAA TATCTTGACA 
GATGATGGGA AAATAAACAG CAATGGGGGT GCACAATTTG AAGGAATGCC AAGATTCACT 
GCTCGAATTT GCATTATTGA AGCACTGAAG GCAAAGGGCT TGTACAAGGG TGCAAAGAAT 
ACCGAAACGA GTTTGGGCAT TTGTTCAAGA ACTAACGACA TAGTGGAGCC TATGATAAAA 
TCCCCGCAGT GGTTTGTTAA TTGTAACACC ATGGCCAAGG TAGCCCTTGA TGCTGTAAGG 
TCTAAGAGGA TTGAGATCAT TCCCCCGCAG TACGAACAAG ACTGGTATAG ATGGCTTGAA 
AATATACGTG ATTGGTGTGT TTCAAGGCAA CATTGGTGGG GACACCGTGT ACCTGCATGG 
TATGTGACAC TAGAAGATGA CCAGGAGAAA ACTCTAGGTT CTGACAATGA CCGCTGGATA 
GTCGCCAAAA GTGAAAGTGC TGCAAGCGTT GAGGCACAGA AAAGTTATCC AGGAAAGAAA 
TTCATCCTAA ATCAGGATCC TGATGTGCTA GACACATGGT TTTCATCTGC TCTCTTCCCC 
TTAGCAGTAC TTGGTTGGCC AGATGATACT GCTGACCTCC GTTCTTTCTA CCCTACTTCA 
GTTCTAGAAA CTGGACTTGA TATTCTCTTC TGTTGGGTAG CACGGATGGT GATGATGGGA 
ACACAACTTG GTGGTGATGT GCCGTTTCAG AAGGTTTATT TGCATCCTAT TGTATGCGAC 
ACACATGGCC GAAAAATGTG CAAGTCACTT GGTAATGTCA TTGATCCTCT TGAGGTGATA 
AATGGCATGA CACTCGAGGG TCTTGTAAAA CGTCTGGAAG AAGGTAATCT GGATCCAGAT 
GAATTAAACC TTGAACGGAA GCTGACAGAT TATCCTGATG GAATTGCTGA ATGTGGTACT 
GATGCCCTCC GCTTTGCACT GATTTCTTAC ACTTCTCAGT CTGACAGGAT AAATCTTGAT 
ATCAAGAGAG TGGTTGGATA CCGACAATGG TGCAATAAAT TGTGGAATGC CATCCGTTTT 
GCCATGGGCA AGCTTGGTGT TCACTACAGT CCACCTGCAA CTGTCGACGT GTCTATCATG 
CCACCAATCT GCAAGTGGAT ACTCTCAGCA CTAAACAAGG CTACCGGCAA AACTGTTACC 
TCTTTAGAAG CATACAAATT CTCTGATGCA ACATCTGCTA TATACTCTTG GTGGCAATAC 
CAGCTATGTG ATGTATTTAT AGAAGCTATA AAACCTTACT TCTTCAACGA ACCTCAAGAG 
TTTGAATCTG CAAGAGTCGC CTCCAGAGAT ACTCTATGGG TTTGCCTGGA GACTGGTTTG 
CGCTTGCTCC ACCCATTCAT GCCTTACATA ACAGAAGAGC TATGGCAGCA TCTTCCACAA 
CCCAAATATT CTTGTAGGCA AGATTCAATT ATGATTTCGG AATATCCATC TCTCGTTGAG 
GAATGGACTA ATGACAATCT TGAAAATGAA ATGGACATTG TCTTAGACAC CGTGAACAAA 
ATAAGGTCTT TGAAGACACG AACAGAAAGA AAAGAAAGAC GTCCTGCTTT TGCACTCTGC 
CGAAGCCAAG ATGTCACTGC CACTATTCAA TGCCATCAAT CTCTAATTGT GTGTCTTTCT 
TCCGTATCAT TTCTTAAGAT CCTGACAGAG AATGACGAAA CTCCAGCTGA TTGTGCAATC 
GCTGTTGTTA ACAAAGATTT GTCGGTGTAC CTTAAGCTTC AAGGAGCCAT AAATGCAGAG 
GCTGAGCGTG AAAAGCTAAG GAAGAAGAGA GATGGAATCC AAAAGCTACA CCATGCTGTG 
ACACACATGA TGGATGCTTC TGGTTATCGC GAGAAGGCAC CTCAAAGTGT CCAGGAAGGG 
GATATGAGAA AGCACACTGC TTTGTTGCGG GAACTGGAGG GCATAAGTGA AGCTGAGAAG 
AAACTAGATG CCAAAACCGA TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632776.1
CDS	594..3623
Translation	MLQAQPALDVLKKVEKKHRGKAVEDENPEDFIDQDTPNGQKKLLAPQMANQYCPSTVEKSWYAWWESSGYFRADSASTKPPFVIVMPPPNVTGALHIGHALTVAIEDAMIRWRRMSGYNALWVPGVDHAGIATQVVVEKKLMRERNLTRHDIGPDNFVCEVLKWKERYGGTILNQLHRLGASLDWSREAFTMDEQRSNAVTEAFVRLHKEGLIYRDNRLVNWDCTLLTSISEIEVDHIDLKEETMLKIPGYATPVQFGVLISFAYPLEEGLGEIVVATTRIETMLGDTAIAVHPEDKRYMHLHGRYAVHPFNGRKLKIICDAEIVDPSFGTGAVKIAPAHDPNDFEVGRRNNLQFINILTDDGKINSNGGAQFEGMPRFTARICIIEALKAKGLYKGAKNTETSLGICSRTNDIVEPMIKSPQWFVNCNTMAKVALDAVRSKRIEIIPPQYEQDWYRWLENIRDWCVSRQHWWGHRVPAWYVTLEDDQEKTLGSDNDRWIVAKSESAASVEAQKSYPGKKFILNQDPDVLDTWFSSALFPLAVLGWPDDTADLRSFYPTSVLETGLDILFCWVARMVMMGTQLGGDVPFQKVYLHPIVCDTHGRKMCKSLGNVIDPLEVINGMTLEGLVKRLEEGNLDPDELNLERKLTDYPDGIAECGTDALRFALISYTSQSDRINLDIKRVVGYRQWCNKLWNAIRFAMGKLGVHYSPPATVDVSIMPPICKWILSALNKATGKTVTSLEAYKFSDATSAIYSWWQYQLCDVFIEAIKPYFFNEPQEFESARVASRDTLWVCLETGLRLLHPFMPYITEELWQHLPQPKYSCRQDSIMISEYPSLVEEWTNDNLENEMDIVLDTVNKIRSLKTRTERKERRPAFALCRSQDVTATIQCHQSLIVCLSSVSFLKILTENDETPADCAIAVVNKDLSVYLKLQGAINAEAEREKLRKKRDGIQKLHHAVTHMMDASGYREKAPQSVQEGDMRKHTALLRELEGISEAEKKLDAKTDNI

Target ORF information:

RefSeq Version	XM_015777290.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777290.2

ORF Insert Sequence:

ATGCTTCAGG CACAGCCAGC TTTAGATGTA CTCAAGAAGG TTGAGAAGAA ACATAGGGGA 
AAAGCTGTGG AGGATGAAAA TCCTGAAGAT TTTATTGATC AAGACACTCC TAATGGGCAG 
AAGAAACTGC TTGCACCTCA AATGGCCAAT CAGTATTGCC CAAGCACGGT TGAGAAATCA 
TGGTATGCCT GGTGGGAATC ATCAGGATAT TTTAGGGCAG ATTCTGCAAG CACAAAGCCA 
CCATTTGTTA TAGTTATGCC ACCTCCAAAT GTGACTGGAG CTCTTCACAT AGGCCATGCT 
CTTACAGTGG CTATAGAGGA TGCGATGATA CGATGGCGGA GGATGTCAGG TTATAATGCT 
CTATGGGTCC CTGGAGTGGA CCATGCTGGC ATTGCTACAC AGGTTGTAGT AGAAAAAAAG 
CTTATGCGTG AAAGGAATCT GACAAGGCAT GACATAGGGC CTGACAATTT TGTTTGTGAA 
GTTCTCAAAT GGAAAGAGCG GTATGGTGGT ACTATATTGA ATCAATTGCA CCGCCTTGGG 
GCCTCACTTG ATTGGTCTCG TGAGGCTTTT ACAATGGATG AGCAAAGGTC AAATGCTGTA 
ACTGAAGCTT TTGTAAGGTT ACACAAGGAA GGCCTGATAT ATAGAGATAA CCGCCTTGTG 
AATTGGGACT GCACACTTCT GACTTCAATT TCTGAGATAG AGGTTGATCA TATTGATCTT 
AAAGAAGAAA CAATGTTAAA GATCCCTGGT TATGCTACTC CTGTACAATT TGGTGTGTTG 
ATATCTTTTG CCTATCCTCT TGAAGAAGGG CTAGGTGAGA TTGTTGTTGC AACAACTCGA 
ATTGAAACAA TGCTTGGTGA TACAGCAATA GCTGTTCATC CTGAGGATAA AAGGTATATG 
CATCTGCATG GACGGTATGC AGTCCACCCC TTCAATGGCC GAAAACTCAA AATAATCTGT 
GATGCTGAGA TAGTTGATCC CAGTTTCGGT ACTGGGGCTG TCAAGATTGC ACCTGCACAT 
GACCCAAATG ACTTTGAGGT TGGAAGACGG AATAACCTTC AGTTCATTAA TATCTTGACA 
GATGATGGGA AAATAAACAG CAATGGGGGT GCACAATTTG AAGGAATGCC AAGATTCACT 
GCTCGAATTT GCATTATTGA AGCACTGAAG GCAAAGGGCT TGTACAAGGG TGCAAAGAAT 
ACCGAAACGA GTTTGGGCAT TTGTTCAAGA ACTAACGACA TAGTGGAGCC TATGATAAAA 
TCCCCGCAGT GGTTTGTTAA TTGTAACACC ATGGCCAAGG TAGCCCTTGA TGCTGTAAGG 
TCTAAGAGGA TTGAGATCAT TCCCCCGCAG TACGAACAAG ACTGGTATAG ATGGCTTGAA 
AATATACGTG ATTGGTGTGT TTCAAGGCAA CATTGGTGGG GACACCGTGT ACCTGCATGG 
TATGTGACAC TAGAAGATGA CCAGGAGAAA ACTCTAGGTT CTGACAATGA CCGCTGGATA 
GTCGCCAAAA GTGAAAGTGC TGCAAGCGTT GAGGCACAGA AAAGTTATCC AGGAAAGAAA 
TTCATCCTAA ATCAGGATCC TGATGTGCTA GACACATGGT TTTCATCTGC TCTCTTCCCC 
TTAGCAGTAC TTGGTTGGCC AGATGATACT GCTGACCTCC GTTCTTTCTA CCCTACTTCA 
GTTCTAGAAA CTGGACTTGA TATTCTCTTC TGTTGGGTAG CACGGATGGT GATGATGGGA 
ACACAACTTG GTGGTGATGT GCCGTTTCAG AAGGTTTATT TGCATCCTAT TGTATGCGAC 
ACACATGGCC GAAAAATGTG CAAGTCACTT GGTAATGTCA TTGATCCTCT TGAGGTGATA 
AATGGCATGA CACTCGAGGG TCTTGTAAAA CGTCTGGAAG AAGGTAATCT GGATCCAGAT 
GAATTAAACC TTGAACGGAA GCTGACAGAT TATCCTGATG GAATTGCTGA ATGTGGTACT 
GATGCCCTCC GCTTTGCACT GATTTCTTAC ACTTCTCAGT CTGACAGGAT AAATCTTGAT 
ATCAAGAGAG TGGTTGGATA CCGACAATGG TGCAATAAAT TGTGGAATGC CATCCGTTTT 
GCCATGGGCA AGCTTGGTGT TCACTACAGT CCACCTGCAA CTGTCGACGT GTCTATCATG 
CCACCAATCT GCAAGTGGAT ACTCTCAGCA CTAAACAAGG CTACCGGCAA AACTGTTACC 
TCTTTAGAAG CATACAAATT CTCTGATGCA ACATCTGCTA TATACTCTTG GTGGCAATAC 
CAGCTATGTG ATGTATTTAT AGAAGCTATA AAACCTTACT TCTTCAACGA ACCTCAAGAG 
TTTGAATCTG CAAGAGTCGC CTCCAGAGAT ACTCTATGGG TTTGCCTGGA GACTGGTTTG 
CGCTTGCTCC ACCCATTCAT GCCTTACATA ACAGAAGAGC TATGGCAGCA TCTTCCACAA 
CCCAAATATT CTTGTAGGCA AGATTCAATT ATGATTTCGG AATATCCATC TCTCGTTGAG 
GAATGGACTA ATGACAATCT TGAAAATGAA ATGGACATTG TCTTAGACAC CGTGAACAAA 
ATAAGGTCTT TGAAGACACG AACAGAAAGA AAAGAAAGAC GTCCTGCTTT TGCACTCTGC 
CGAAGCCAAG ATGTCACTGC CACTATTCAA TGCCATCAAT CTCTAATTGT GTGTCTTTCT 
TCCGTATCAT TTCTTAAGAT CCTGACAGAG AATGACGAAA CTCCAGCTGA TTGTGCAATC 
GCTGTTGTTA ACAAAGATTT GTCGGTGTAC CTTAAGCTTC AAGGAGCCAT AAATGCAGAG 
GCTGAGCGTG AAAAGCTAAG GAAGAAGAGA GATGGAATCC AAAAGCTACA CCATGCTGTG 
ACACACATGA TGGATGCTTC TGGTTATCGC GAGAAGGCAC CTCAAAGTGT CCAGGAAGGG 
GATATGAGAA AGCACACTGC TTTGTTGCGG GAACTGGAGG GCATAAGTGA AGCTGAGAAG 
AAACTAGATG CCAAAACCGA TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332758 ( XM_026024198.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_025879983.1 Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOa30948
Clone ID Related Accession (Same CDS sequence)	XM_015777290.2 , XM_026024198.1 , XM_015777290.1
Accession Version	XM_026024198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	valine--tRNA ligase, mitochondrial 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCAGG CACAGCCAGC TTTAGATGTA CTCAAGAAGG TTGAGAAGAA ACATAGGGGA 
AAAGCTGTGG AGGATGAAAA TCCTGAAGAT TTTATTGATC AAGACACTCC TAATGGGCAG 
AAGAAACTGC TTGCACCTCA AATGGCCAAT CAGTATTGCC CAAGCACGGT TGAGAAATCA 
TGGTATGCCT GGTGGGAATC ATCAGGATAT TTTAGGGCAG ATTCTGCAAG CACAAAGCCA 
CCATTTGTTA TAGTTATGCC ACCTCCAAAT GTGACTGGAG CTCTTCACAT AGGCCATGCT 
CTTACAGTGG CTATAGAGGA TGCGATGATA CGATGGCGGA GGATGTCAGG TTATAATGCT 
CTATGGGTCC CTGGAGTGGA CCATGCTGGC ATTGCTACAC AGGTTGTAGT AGAAAAAAAG 
CTTATGCGTG AAAGGAATCT GACAAGGCAT GACATAGGGC CTGACAATTT TGTTTGTGAA 
GTTCTCAAAT GGAAAGAGCG GTATGGTGGT ACTATATTGA ATCAATTGCA CCGCCTTGGG 
GCCTCACTTG ATTGGTCTCG TGAGGCTTTT ACAATGGATG AGCAAAGGTC AAATGCTGTA 
ACTGAAGCTT TTGTAAGGTT ACACAAGGAA GGCCTGATAT ATAGAGATAA CCGCCTTGTG 
AATTGGGACT GCACACTTCT GACTTCAATT TCTGAGATAG AGGTTGATCA TATTGATCTT 
AAAGAAGAAA CAATGTTAAA GATCCCTGGT TATGCTACTC CTGTACAATT TGGTGTGTTG 
ATATCTTTTG CCTATCCTCT TGAAGAAGGG CTAGGTGAGA TTGTTGTTGC AACAACTCGA 
ATTGAAACAA TGCTTGGTGA TACAGCAATA GCTGTTCATC CTGAGGATAA AAGGTATATG 
CATCTGCATG GACGGTATGC AGTCCACCCC TTCAATGGCC GAAAACTCAA AATAATCTGT 
GATGCTGAGA TAGTTGATCC CAGTTTCGGT ACTGGGGCTG TCAAGATTGC ACCTGCACAT 
GACCCAAATG ACTTTGAGGT TGGAAGACGG AATAACCTTC AGTTCATTAA TATCTTGACA 
GATGATGGGA AAATAAACAG CAATGGGGGT GCACAATTTG AAGGAATGCC AAGATTCACT 
GCTCGAATTT GCATTATTGA AGCACTGAAG GCAAAGGGCT TGTACAAGGG TGCAAAGAAT 
ACCGAAACGA GTTTGGGCAT TTGTTCAAGA ACTAACGACA TAGTGGAGCC TATGATAAAA 
TCCCCGCAGT GGTTTGTTAA TTGTAACACC ATGGCCAAGG TAGCCCTTGA TGCTGTAAGG 
TCTAAGAGGA TTGAGATCAT TCCCCCGCAG TACGAACAAG ACTGGTATAG ATGGCTTGAA 
AATATACGTG ATTGGTGTGT TTCAAGGCAA CATTGGTGGG GACACCGTGT ACCTGCATGG 
TATGTGACAC TAGAAGATGA CCAGGAGAAA ACTCTAGGTT CTGACAATGA CCGCTGGATA 
GTCGCCAAAA GTGAAAGTGC TGCAAGCGTT GAGGCACAGA AAAGTTATCC AGGAAAGAAA 
TTCATCCTAA ATCAGGATCC TGATGTGCTA GACACATGGT TTTCATCTGC TCTCTTCCCC 
TTAGCAGTAC TTGGTTGGCC AGATGATACT GCTGACCTCC GTTCTTTCTA CCCTACTTCA 
GTTCTAGAAA CTGGACTTGA TATTCTCTTC TGTTGGGTAG CACGGATGGT GATGATGGGA 
ACACAACTTG GTGGTGATGT GCCGTTTCAG AAGGTTTATT TGCATCCTAT TGTATGCGAC 
ACACATGGCC GAAAAATGTG CAAGTCACTT GGTAATGTCA TTGATCCTCT TGAGGTGATA 
AATGGCATGA CACTCGAGGG TCTTGTAAAA CGTCTGGAAG AAGGTAATCT GGATCCAGAT 
GAATTAAACC TTGAACGGAA GCTGACAGAT TATCCTGATG GAATTGCTGA ATGTGGTACT 
GATGCCCTCC GCTTTGCACT GATTTCTTAC ACTTCTCAGT CTGACAGGAT AAATCTTGAT 
ATCAAGAGAG TGGTTGGATA CCGACAATGG TGCAATAAAT TGTGGAATGC CATCCGTTTT 
GCCATGGGCA AGCTTGGTGT TCACTACAGT CCACCTGCAA CTGTCGACGT GTCTATCATG 
CCACCAATCT GCAAGTGGAT ACTCTCAGCA CTAAACAAGG CTACCGGCAA AACTGTTACC 
TCTTTAGAAG CATACAAATT CTCTGATGCA ACATCTGCTA TATACTCTTG GTGGCAATAC 
CAGCTATGTG ATGTATTTAT AGAAGCTATA AAACCTTACT TCTTCAACGA ACCTCAAGAG 
TTTGAATCTG CAAGAGTCGC CTCCAGAGAT ACTCTATGGG TTTGCCTGGA GACTGGTTTG 
CGCTTGCTCC ACCCATTCAT GCCTTACATA ACAGAAGAGC TATGGCAGCA TCTTCCACAA 
CCCAAATATT CTTGTAGGCA AGATTCAATT ATGATTTCGG AATATCCATC TCTCGTTGAG 
GAATGGACTA ATGACAATCT TGAAAATGAA ATGGACATTG TCTTAGACAC CGTGAACAAA 
ATAAGGTCTT TGAAGACACG AACAGAAAGA AAAGAAAGAC GTCCTGCTTT TGCACTCTGC 
CGAAGCCAAG ATGTCACTGC CACTATTCAA TGCCATCAAT CTCTAATTGT GTGTCTTTCT 
TCCGTATCAT TTCTTAAGAT CCTGACAGAG AATGACGAAA CTCCAGCTGA TTGTGCAATC 
GCTGTTGTTA ACAAAGATTT GTCGGTGTAC CTTAAGCTTC AAGGAGCCAT AAATGCAGAG 
GCTGAGCGTG AAAAGCTAAG GAAGAAGAGA GATGGAATCC AAAAGCTACA CCATGCTGTG 
ACACACATGA TGGATGCTTC TGGTTATCGC GAGAAGGCAC CTCAAAGTGT CCAGGAAGGG 
GATATGAGAA AGCACACTGC TTTGTTGCGG GAACTGGAGG GCATAAGTGA AGCTGAGAAG 
AAACTAGATG CCAAAACCGA TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025879983.1
CDS	591..3620
Translation	MLQAQPALDVLKKVEKKHRGKAVEDENPEDFIDQDTPNGQKKLLAPQMANQYCPSTVEKSWYAWWESSGYFRADSASTKPPFVIVMPPPNVTGALHIGHALTVAIEDAMIRWRRMSGYNALWVPGVDHAGIATQVVVEKKLMRERNLTRHDIGPDNFVCEVLKWKERYGGTILNQLHRLGASLDWSREAFTMDEQRSNAVTEAFVRLHKEGLIYRDNRLVNWDCTLLTSISEIEVDHIDLKEETMLKIPGYATPVQFGVLISFAYPLEEGLGEIVVATTRIETMLGDTAIAVHPEDKRYMHLHGRYAVHPFNGRKLKIICDAEIVDPSFGTGAVKIAPAHDPNDFEVGRRNNLQFINILTDDGKINSNGGAQFEGMPRFTARICIIEALKAKGLYKGAKNTETSLGICSRTNDIVEPMIKSPQWFVNCNTMAKVALDAVRSKRIEIIPPQYEQDWYRWLENIRDWCVSRQHWWGHRVPAWYVTLEDDQEKTLGSDNDRWIVAKSESAASVEAQKSYPGKKFILNQDPDVLDTWFSSALFPLAVLGWPDDTADLRSFYPTSVLETGLDILFCWVARMVMMGTQLGGDVPFQKVYLHPIVCDTHGRKMCKSLGNVIDPLEVINGMTLEGLVKRLEEGNLDPDELNLERKLTDYPDGIAECGTDALRFALISYTSQSDRINLDIKRVVGYRQWCNKLWNAIRFAMGKLGVHYSPPATVDVSIMPPICKWILSALNKATGKTVTSLEAYKFSDATSAIYSWWQYQLCDVFIEAIKPYFFNEPQEFESARVASRDTLWVCLETGLRLLHPFMPYITEELWQHLPQPKYSCRQDSIMISEYPSLVEEWTNDNLENEMDIVLDTVNKIRSLKTRTERKERRPAFALCRSQDVTATIQCHQSLIVCLSSVSFLKILTENDETPADCAIAVVNKDLSVYLKLQGAINAEAEREKLRKKRDGIQKLHHAVTHMMDASGYREKAPQSVQEGDMRKHTALLRELEGISEAEKKLDAKTDNI

Target ORF information:

RefSeq Version	XM_026024198.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026024198.1

ORF Insert Sequence:

ATGCTTCAGG CACAGCCAGC TTTAGATGTA CTCAAGAAGG TTGAGAAGAA ACATAGGGGA 
AAAGCTGTGG AGGATGAAAA TCCTGAAGAT TTTATTGATC AAGACACTCC TAATGGGCAG 
AAGAAACTGC TTGCACCTCA AATGGCCAAT CAGTATTGCC CAAGCACGGT TGAGAAATCA 
TGGTATGCCT GGTGGGAATC ATCAGGATAT TTTAGGGCAG ATTCTGCAAG CACAAAGCCA 
CCATTTGTTA TAGTTATGCC ACCTCCAAAT GTGACTGGAG CTCTTCACAT AGGCCATGCT 
CTTACAGTGG CTATAGAGGA TGCGATGATA CGATGGCGGA GGATGTCAGG TTATAATGCT 
CTATGGGTCC CTGGAGTGGA CCATGCTGGC ATTGCTACAC AGGTTGTAGT AGAAAAAAAG 
CTTATGCGTG AAAGGAATCT GACAAGGCAT GACATAGGGC CTGACAATTT TGTTTGTGAA 
GTTCTCAAAT GGAAAGAGCG GTATGGTGGT ACTATATTGA ATCAATTGCA CCGCCTTGGG 
GCCTCACTTG ATTGGTCTCG TGAGGCTTTT ACAATGGATG AGCAAAGGTC AAATGCTGTA 
ACTGAAGCTT TTGTAAGGTT ACACAAGGAA GGCCTGATAT ATAGAGATAA CCGCCTTGTG 
AATTGGGACT GCACACTTCT GACTTCAATT TCTGAGATAG AGGTTGATCA TATTGATCTT 
AAAGAAGAAA CAATGTTAAA GATCCCTGGT TATGCTACTC CTGTACAATT TGGTGTGTTG 
ATATCTTTTG CCTATCCTCT TGAAGAAGGG CTAGGTGAGA TTGTTGTTGC AACAACTCGA 
ATTGAAACAA TGCTTGGTGA TACAGCAATA GCTGTTCATC CTGAGGATAA AAGGTATATG 
CATCTGCATG GACGGTATGC AGTCCACCCC TTCAATGGCC GAAAACTCAA AATAATCTGT 
GATGCTGAGA TAGTTGATCC CAGTTTCGGT ACTGGGGCTG TCAAGATTGC ACCTGCACAT 
GACCCAAATG ACTTTGAGGT TGGAAGACGG AATAACCTTC AGTTCATTAA TATCTTGACA 
GATGATGGGA AAATAAACAG CAATGGGGGT GCACAATTTG AAGGAATGCC AAGATTCACT 
GCTCGAATTT GCATTATTGA AGCACTGAAG GCAAAGGGCT TGTACAAGGG TGCAAAGAAT 
ACCGAAACGA GTTTGGGCAT TTGTTCAAGA ACTAACGACA TAGTGGAGCC TATGATAAAA 
TCCCCGCAGT GGTTTGTTAA TTGTAACACC ATGGCCAAGG TAGCCCTTGA TGCTGTAAGG 
TCTAAGAGGA TTGAGATCAT TCCCCCGCAG TACGAACAAG ACTGGTATAG ATGGCTTGAA 
AATATACGTG ATTGGTGTGT TTCAAGGCAA CATTGGTGGG GACACCGTGT ACCTGCATGG 
TATGTGACAC TAGAAGATGA CCAGGAGAAA ACTCTAGGTT CTGACAATGA CCGCTGGATA 
GTCGCCAAAA GTGAAAGTGC TGCAAGCGTT GAGGCACAGA AAAGTTATCC AGGAAAGAAA 
TTCATCCTAA ATCAGGATCC TGATGTGCTA GACACATGGT TTTCATCTGC TCTCTTCCCC 
TTAGCAGTAC TTGGTTGGCC AGATGATACT GCTGACCTCC GTTCTTTCTA CCCTACTTCA 
GTTCTAGAAA CTGGACTTGA TATTCTCTTC TGTTGGGTAG CACGGATGGT GATGATGGGA 
ACACAACTTG GTGGTGATGT GCCGTTTCAG AAGGTTTATT TGCATCCTAT TGTATGCGAC 
ACACATGGCC GAAAAATGTG CAAGTCACTT GGTAATGTCA TTGATCCTCT TGAGGTGATA 
AATGGCATGA CACTCGAGGG TCTTGTAAAA CGTCTGGAAG AAGGTAATCT GGATCCAGAT 
GAATTAAACC TTGAACGGAA GCTGACAGAT TATCCTGATG GAATTGCTGA ATGTGGTACT 
GATGCCCTCC GCTTTGCACT GATTTCTTAC ACTTCTCAGT CTGACAGGAT AAATCTTGAT 
ATCAAGAGAG TGGTTGGATA CCGACAATGG TGCAATAAAT TGTGGAATGC CATCCGTTTT 
GCCATGGGCA AGCTTGGTGT TCACTACAGT CCACCTGCAA CTGTCGACGT GTCTATCATG 
CCACCAATCT GCAAGTGGAT ACTCTCAGCA CTAAACAAGG CTACCGGCAA AACTGTTACC 
TCTTTAGAAG CATACAAATT CTCTGATGCA ACATCTGCTA TATACTCTTG GTGGCAATAC 
CAGCTATGTG ATGTATTTAT AGAAGCTATA AAACCTTACT TCTTCAACGA ACCTCAAGAG 
TTTGAATCTG CAAGAGTCGC CTCCAGAGAT ACTCTATGGG TTTGCCTGGA GACTGGTTTG 
CGCTTGCTCC ACCCATTCAT GCCTTACATA ACAGAAGAGC TATGGCAGCA TCTTCCACAA 
CCCAAATATT CTTGTAGGCA AGATTCAATT ATGATTTCGG AATATCCATC TCTCGTTGAG 
GAATGGACTA ATGACAATCT TGAAAATGAA ATGGACATTG TCTTAGACAC CGTGAACAAA 
ATAAGGTCTT TGAAGACACG AACAGAAAGA AAAGAAAGAC GTCCTGCTTT TGCACTCTGC 
CGAAGCCAAG ATGTCACTGC CACTATTCAA TGCCATCAAT CTCTAATTGT GTGTCTTTCT 
TCCGTATCAT TTCTTAAGAT CCTGACAGAG AATGACGAAA CTCCAGCTGA TTGTGCAATC 
GCTGTTGTTA ACAAAGATTT GTCGGTGTAC CTTAAGCTTC AAGGAGCCAT AAATGCAGAG 
GCTGAGCGTG AAAAGCTAAG GAAGAAGAGA GATGGAATCC AAAAGCTACA CCATGCTGTG 
ACACACATGA TGGATGCTTC TGGTTATCGC GAGAAGGCAC CTCAAAGTGT CCAGGAAGGG 
GATATGAGAA AGCACACTGC TTTGTTGCGG GAACTGGAGG GCATAAGTGA AGCTGAGAAG 
AAACTAGATG CCAAAACCGA TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332758 ( XM_015777290.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015632776.1 Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X4, mRNA.

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CloneID	OOa30948
Clone ID Related Accession (Same CDS sequence)	XM_015777290.2 , XM_026024198.1 , XM_015777290.1
Accession Version	XM_015777290.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	valine--tRNA ligase, mitochondrial 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCAGG CACAGCCAGC TTTAGATGTA CTCAAGAAGG TTGAGAAGAA ACATAGGGGA 
AAAGCTGTGG AGGATGAAAA TCCTGAAGAT TTTATTGATC AAGACACTCC TAATGGGCAG 
AAGAAACTGC TTGCACCTCA AATGGCCAAT CAGTATTGCC CAAGCACGGT TGAGAAATCA 
TGGTATGCCT GGTGGGAATC ATCAGGATAT TTTAGGGCAG ATTCTGCAAG CACAAAGCCA 
CCATTTGTTA TAGTTATGCC ACCTCCAAAT GTGACTGGAG CTCTTCACAT AGGCCATGCT 
CTTACAGTGG CTATAGAGGA TGCGATGATA CGATGGCGGA GGATGTCAGG TTATAATGCT 
CTATGGGTCC CTGGAGTGGA CCATGCTGGC ATTGCTACAC AGGTTGTAGT AGAAAAAAAG 
CTTATGCGTG AAAGGAATCT GACAAGGCAT GACATAGGGC CTGACAATTT TGTTTGTGAA 
GTTCTCAAAT GGAAAGAGCG GTATGGTGGT ACTATATTGA ATCAATTGCA CCGCCTTGGG 
GCCTCACTTG ATTGGTCTCG TGAGGCTTTT ACAATGGATG AGCAAAGGTC AAATGCTGTA 
ACTGAAGCTT TTGTAAGGTT ACACAAGGAA GGCCTGATAT ATAGAGATAA CCGCCTTGTG 
AATTGGGACT GCACACTTCT GACTTCAATT TCTGAGATAG AGGTTGATCA TATTGATCTT 
AAAGAAGAAA CAATGTTAAA GATCCCTGGT TATGCTACTC CTGTACAATT TGGTGTGTTG 
ATATCTTTTG CCTATCCTCT TGAAGAAGGG CTAGGTGAGA TTGTTGTTGC AACAACTCGA 
ATTGAAACAA TGCTTGGTGA TACAGCAATA GCTGTTCATC CTGAGGATAA AAGGTATATG 
CATCTGCATG GACGGTATGC AGTCCACCCC TTCAATGGCC GAAAACTCAA AATAATCTGT 
GATGCTGAGA TAGTTGATCC CAGTTTCGGT ACTGGGGCTG TCAAGATTGC ACCTGCACAT 
GACCCAAATG ACTTTGAGGT TGGAAGACGG AATAACCTTC AGTTCATTAA TATCTTGACA 
GATGATGGGA AAATAAACAG CAATGGGGGT GCACAATTTG AAGGAATGCC AAGATTCACT 
GCTCGAATTT GCATTATTGA AGCACTGAAG GCAAAGGGCT TGTACAAGGG TGCAAAGAAT 
ACCGAAACGA GTTTGGGCAT TTGTTCAAGA ACTAACGACA TAGTGGAGCC TATGATAAAA 
TCCCCGCAGT GGTTTGTTAA TTGTAACACC ATGGCCAAGG TAGCCCTTGA TGCTGTAAGG 
TCTAAGAGGA TTGAGATCAT TCCCCCGCAG TACGAACAAG ACTGGTATAG ATGGCTTGAA 
AATATACGTG ATTGGTGTGT TTCAAGGCAA CATTGGTGGG GACACCGTGT ACCTGCATGG 
TATGTGACAC TAGAAGATGA CCAGGAGAAA ACTCTAGGTT CTGACAATGA CCGCTGGATA 
GTCGCCAAAA GTGAAAGTGC TGCAAGCGTT GAGGCACAGA AAAGTTATCC AGGAAAGAAA 
TTCATCCTAA ATCAGGATCC TGATGTGCTA GACACATGGT TTTCATCTGC TCTCTTCCCC 
TTAGCAGTAC TTGGTTGGCC AGATGATACT GCTGACCTCC GTTCTTTCTA CCCTACTTCA 
GTTCTAGAAA CTGGACTTGA TATTCTCTTC TGTTGGGTAG CACGGATGGT GATGATGGGA 
ACACAACTTG GTGGTGATGT GCCGTTTCAG AAGGTTTATT TGCATCCTAT TGTATGCGAC 
ACACATGGCC GAAAAATGTG CAAGTCACTT GGTAATGTCA TTGATCCTCT TGAGGTGATA 
AATGGCATGA CACTCGAGGG TCTTGTAAAA CGTCTGGAAG AAGGTAATCT GGATCCAGAT 
GAATTAAACC TTGAACGGAA GCTGACAGAT TATCCTGATG GAATTGCTGA ATGTGGTACT 
GATGCCCTCC GCTTTGCACT GATTTCTTAC ACTTCTCAGT CTGACAGGAT AAATCTTGAT 
ATCAAGAGAG TGGTTGGATA CCGACAATGG TGCAATAAAT TGTGGAATGC CATCCGTTTT 
GCCATGGGCA AGCTTGGTGT TCACTACAGT CCACCTGCAA CTGTCGACGT GTCTATCATG 
CCACCAATCT GCAAGTGGAT ACTCTCAGCA CTAAACAAGG CTACCGGCAA AACTGTTACC 
TCTTTAGAAG CATACAAATT CTCTGATGCA ACATCTGCTA TATACTCTTG GTGGCAATAC 
CAGCTATGTG ATGTATTTAT AGAAGCTATA AAACCTTACT TCTTCAACGA ACCTCAAGAG 
TTTGAATCTG CAAGAGTCGC CTCCAGAGAT ACTCTATGGG TTTGCCTGGA GACTGGTTTG 
CGCTTGCTCC ACCCATTCAT GCCTTACATA ACAGAAGAGC TATGGCAGCA TCTTCCACAA 
CCCAAATATT CTTGTAGGCA AGATTCAATT ATGATTTCGG AATATCCATC TCTCGTTGAG 
GAATGGACTA ATGACAATCT TGAAAATGAA ATGGACATTG TCTTAGACAC CGTGAACAAA 
ATAAGGTCTT TGAAGACACG AACAGAAAGA AAAGAAAGAC GTCCTGCTTT TGCACTCTGC 
CGAAGCCAAG ATGTCACTGC CACTATTCAA TGCCATCAAT CTCTAATTGT GTGTCTTTCT 
TCCGTATCAT TTCTTAAGAT CCTGACAGAG AATGACGAAA CTCCAGCTGA TTGTGCAATC 
GCTGTTGTTA ACAAAGATTT GTCGGTGTAC CTTAAGCTTC AAGGAGCCAT AAATGCAGAG 
GCTGAGCGTG AAAAGCTAAG GAAGAAGAGA GATGGAATCC AAAAGCTACA CCATGCTGTG 
ACACACATGA TGGATGCTTC TGGTTATCGC GAGAAGGCAC CTCAAAGTGT CCAGGAAGGG 
GATATGAGAA AGCACACTGC TTTGTTGCGG GAACTGGAGG GCATAAGTGA AGCTGAGAAG 
AAACTAGATG CCAAAACCGA TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015632776.1
CDS	591..3620
Translation	MLQAQPALDVLKKVEKKHRGKAVEDENPEDFIDQDTPNGQKKLLAPQMANQYCPSTVEKSWYAWWESSGYFRADSASTKPPFVIVMPPPNVTGALHIGHALTVAIEDAMIRWRRMSGYNALWVPGVDHAGIATQVVVEKKLMRERNLTRHDIGPDNFVCEVLKWKERYGGTILNQLHRLGASLDWSREAFTMDEQRSNAVTEAFVRLHKEGLIYRDNRLVNWDCTLLTSISEIEVDHIDLKEETMLKIPGYATPVQFGVLISFAYPLEEGLGEIVVATTRIETMLGDTAIAVHPEDKRYMHLHGRYAVHPFNGRKLKIICDAEIVDPSFGTGAVKIAPAHDPNDFEVGRRNNLQFINILTDDGKINSNGGAQFEGMPRFTARICIIEALKAKGLYKGAKNTETSLGICSRTNDIVEPMIKSPQWFVNCNTMAKVALDAVRSKRIEIIPPQYEQDWYRWLENIRDWCVSRQHWWGHRVPAWYVTLEDDQEKTLGSDNDRWIVAKSESAASVEAQKSYPGKKFILNQDPDVLDTWFSSALFPLAVLGWPDDTADLRSFYPTSVLETGLDILFCWVARMVMMGTQLGGDVPFQKVYLHPIVCDTHGRKMCKSLGNVIDPLEVINGMTLEGLVKRLEEGNLDPDELNLERKLTDYPDGIAECGTDALRFALISYTSQSDRINLDIKRVVGYRQWCNKLWNAIRFAMGKLGVHYSPPATVDVSIMPPICKWILSALNKATGKTVTSLEAYKFSDATSAIYSWWQYQLCDVFIEAIKPYFFNEPQEFESARVASRDTLWVCLETGLRLLHPFMPYITEELWQHLPQPKYSCRQDSIMISEYPSLVEEWTNDNLENEMDIVLDTVNKIRSLKTRTERKERRPAFALCRSQDVTATIQCHQSLIVCLSSVSFLKILTENDETPADCAIAVVNKDLSVYLKLQGAINAEAEREKLRKKRDGIQKLHHAVTHMMDASGYREKAPQSVQEGDMRKHTALLRELEGISEAEKKLDAKTDNI

Target ORF information:

RefSeq Version	XM_015777290.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group valine--tRNA ligase, mitochondrial 1 (LOC4332758), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015777290.1

ORF Insert Sequence:

ATGCTTCAGG CACAGCCAGC TTTAGATGTA CTCAAGAAGG TTGAGAAGAA ACATAGGGGA 
AAAGCTGTGG AGGATGAAAA TCCTGAAGAT TTTATTGATC AAGACACTCC TAATGGGCAG 
AAGAAACTGC TTGCACCTCA AATGGCCAAT CAGTATTGCC CAAGCACGGT TGAGAAATCA 
TGGTATGCCT GGTGGGAATC ATCAGGATAT TTTAGGGCAG ATTCTGCAAG CACAAAGCCA 
CCATTTGTTA TAGTTATGCC ACCTCCAAAT GTGACTGGAG CTCTTCACAT AGGCCATGCT 
CTTACAGTGG CTATAGAGGA TGCGATGATA CGATGGCGGA GGATGTCAGG TTATAATGCT 
CTATGGGTCC CTGGAGTGGA CCATGCTGGC ATTGCTACAC AGGTTGTAGT AGAAAAAAAG 
CTTATGCGTG AAAGGAATCT GACAAGGCAT GACATAGGGC CTGACAATTT TGTTTGTGAA 
GTTCTCAAAT GGAAAGAGCG GTATGGTGGT ACTATATTGA ATCAATTGCA CCGCCTTGGG 
GCCTCACTTG ATTGGTCTCG TGAGGCTTTT ACAATGGATG AGCAAAGGTC AAATGCTGTA 
ACTGAAGCTT TTGTAAGGTT ACACAAGGAA GGCCTGATAT ATAGAGATAA CCGCCTTGTG 
AATTGGGACT GCACACTTCT GACTTCAATT TCTGAGATAG AGGTTGATCA TATTGATCTT 
AAAGAAGAAA CAATGTTAAA GATCCCTGGT TATGCTACTC CTGTACAATT TGGTGTGTTG 
ATATCTTTTG CCTATCCTCT TGAAGAAGGG CTAGGTGAGA TTGTTGTTGC AACAACTCGA 
ATTGAAACAA TGCTTGGTGA TACAGCAATA GCTGTTCATC CTGAGGATAA AAGGTATATG 
CATCTGCATG GACGGTATGC AGTCCACCCC TTCAATGGCC GAAAACTCAA AATAATCTGT 
GATGCTGAGA TAGTTGATCC CAGTTTCGGT ACTGGGGCTG TCAAGATTGC ACCTGCACAT 
GACCCAAATG ACTTTGAGGT TGGAAGACGG AATAACCTTC AGTTCATTAA TATCTTGACA 
GATGATGGGA AAATAAACAG CAATGGGGGT GCACAATTTG AAGGAATGCC AAGATTCACT 
GCTCGAATTT GCATTATTGA AGCACTGAAG GCAAAGGGCT TGTACAAGGG TGCAAAGAAT 
ACCGAAACGA GTTTGGGCAT TTGTTCAAGA ACTAACGACA TAGTGGAGCC TATGATAAAA 
TCCCCGCAGT GGTTTGTTAA TTGTAACACC ATGGCCAAGG TAGCCCTTGA TGCTGTAAGG 
TCTAAGAGGA TTGAGATCAT TCCCCCGCAG TACGAACAAG ACTGGTATAG ATGGCTTGAA 
AATATACGTG ATTGGTGTGT TTCAAGGCAA CATTGGTGGG GACACCGTGT ACCTGCATGG 
TATGTGACAC TAGAAGATGA CCAGGAGAAA ACTCTAGGTT CTGACAATGA CCGCTGGATA 
GTCGCCAAAA GTGAAAGTGC TGCAAGCGTT GAGGCACAGA AAAGTTATCC AGGAAAGAAA 
TTCATCCTAA ATCAGGATCC TGATGTGCTA GACACATGGT TTTCATCTGC TCTCTTCCCC 
TTAGCAGTAC TTGGTTGGCC AGATGATACT GCTGACCTCC GTTCTTTCTA CCCTACTTCA 
GTTCTAGAAA CTGGACTTGA TATTCTCTTC TGTTGGGTAG CACGGATGGT GATGATGGGA 
ACACAACTTG GTGGTGATGT GCCGTTTCAG AAGGTTTATT TGCATCCTAT TGTATGCGAC 
ACACATGGCC GAAAAATGTG CAAGTCACTT GGTAATGTCA TTGATCCTCT TGAGGTGATA 
AATGGCATGA CACTCGAGGG TCTTGTAAAA CGTCTGGAAG AAGGTAATCT GGATCCAGAT 
GAATTAAACC TTGAACGGAA GCTGACAGAT TATCCTGATG GAATTGCTGA ATGTGGTACT 
GATGCCCTCC GCTTTGCACT GATTTCTTAC ACTTCTCAGT CTGACAGGAT AAATCTTGAT 
ATCAAGAGAG TGGTTGGATA CCGACAATGG TGCAATAAAT TGTGGAATGC CATCCGTTTT 
GCCATGGGCA AGCTTGGTGT TCACTACAGT CCACCTGCAA CTGTCGACGT GTCTATCATG 
CCACCAATCT GCAAGTGGAT ACTCTCAGCA CTAAACAAGG CTACCGGCAA AACTGTTACC 
TCTTTAGAAG CATACAAATT CTCTGATGCA ACATCTGCTA TATACTCTTG GTGGCAATAC 
CAGCTATGTG ATGTATTTAT AGAAGCTATA AAACCTTACT TCTTCAACGA ACCTCAAGAG 
TTTGAATCTG CAAGAGTCGC CTCCAGAGAT ACTCTATGGG TTTGCCTGGA GACTGGTTTG 
CGCTTGCTCC ACCCATTCAT GCCTTACATA ACAGAAGAGC TATGGCAGCA TCTTCCACAA 
CCCAAATATT CTTGTAGGCA AGATTCAATT ATGATTTCGG AATATCCATC TCTCGTTGAG 
GAATGGACTA ATGACAATCT TGAAAATGAA ATGGACATTG TCTTAGACAC CGTGAACAAA 
ATAAGGTCTT TGAAGACACG AACAGAAAGA AAAGAAAGAC GTCCTGCTTT TGCACTCTGC 
CGAAGCCAAG ATGTCACTGC CACTATTCAA TGCCATCAAT CTCTAATTGT GTGTCTTTCT 
TCCGTATCAT TTCTTAAGAT CCTGACAGAG AATGACGAAA CTCCAGCTGA TTGTGCAATC 
GCTGTTGTTA ACAAAGATTT GTCGGTGTAC CTTAAGCTTC AAGGAGCCAT AAATGCAGAG 
GCTGAGCGTG AAAAGCTAAG GAAGAAGAGA GATGGAATCC AAAAGCTACA CCATGCTGTG 
ACACACATGA TGGATGCTTC TGGTTATCGC GAGAAGGCAC CTCAAAGTGT CCAGGAAGGG 
GATATGAGAA AGCACACTGC TTTGTTGCGG GAACTGGAGG GCATAAGTGA AGCTGAGAAG 
AAACTAGATG CCAAAACCGA TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC4332758 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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