SPHK2 cDNA ORF clone, Orycteropus afer afer

Gene
Clones
gRNAs

Summary

Gene Symbol	SPHK2
Entrez Gene ID	103212115
Full Name	sphingosine kinase 2
Gene Type	protein-coding
Organism	Orycteropus afer afer

mRNA and Protein(s)

mRNA	Protein	Name
XM_007958114.1	XP_007956305.1	sphingosine kinase 2

Pathways from BioSystems
Gene Ontology

The following SPHK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPHK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOr125247	XM_007958114.1 Latest version!	Orycteropus afer afer sphingosine kinase 2 (SPHK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPHK2 ( XM_007958114.1 ) cDNA ORF clone, Orycteropus afer afer -> XP_007956305.1 Orycteropus afer afer sphingosine kinase 2 (SPHK2), mRNA.

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CloneID	OOr125247
Clone ID Related Accession (Same CDS sequence)	XM_007958114.1
Accession Version	XM_007958114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Orycteropus afer afer
Product	sphingosine kinase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006922441.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orycteropus afer afer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCCCGC CATTACCACA ACTGGCCGCC AGCACCCCAC TCCTGCACGG GGAGTTCGGC 
TCCTACCCGG CCCGTGGCCC CCGCTTCGCC CTCACGCTCA CGCCGCAGGC CCTGTACATC 
CAGCGACTGC GTCCCAAGCC TGAAGCCCGG CCCCGGGGCG GCCTGGTCCC GCTGGCCGAG 
GTCTCAGGCT GCTGCACCCT ACGCAGCCGC AGCCCACTGG ACTCTGCAGC CTACTTCTGC 
GTCTACACCT ACCCGCAGGG CCGGCGCGGG GCCCGGCGCA GAGCCACGCG CACCTTCCGG 
GCCGACGGGG CAGCCACCTA CGAGGAGAAC CGCACCGAGG CCCATCGCTG GGCGGTCACG 
CTCACATGCC TGCTCCGTGG GCTGCCACTG CCTGGGGATG GCGATATCAC CCCTGACCTG 
CTGCCCCGTC CGCCCCGTTT GCTTTTATTG GTCAACCCCT TTGGGGGGCG GGGCCTGGCC 
TGGCAGTGGT GTAAGAACCA CGTGTTGCCT ATGATCTCAG AAGCTGGGCT GTCCTTCAAC 
CTCATGCAGA CAGAGCGGCC AAACCACGCC CGGGAGCTGG TCCAGGCGCT GAGCCTGAGC 
GAGTGGGACG GCATCATCAC GGTCTCGGGA GACGGGCTGC TCTATGAGGT GCTGAACGGG 
CTCCTCGATC GCCCCGACTG GGAGGAGGCA GTGAGGATGC CCGTGGGCAT CCTCCCCTGC 
GGCTCGGGCA ACGCGCTGGC GGGCGCCGTG AACCTGCACG GGGGGTTTGC ACCAGCCCTG 
GGCCTCGACC TTCTGCTTAA CTGCTCGCTG CTGCTCTGCC GGGGTGGCAG CCACCCACTG 
GACCTGCTCT CTGTGACGCT GGCTTCCGGC TCCCGCTGTT TCTCCTTCCT GTCCGTGGCC 
TGGGGCTTCG TGTCCGACGT GGACATCCAG AGTGAGCGCT TCAGGGCCCT GGGCAGCGCC 
CGCTTCACTC TGGGCACAGT GCTGGGCCTC GCCACGCTGC ACACCTACCG AGGGCGGCTC 
TCCTACCTCC CGGCCACCGG GGAACCCGCC TCGCCCCCGC CCGCCCACGG CCTGCCCCGG 
GCCAAGTCGG AGCTGACCCT GGCCCCAGAG CCAGCCCCGC CCGTGACCCA CTCGCCTCTG 
CACCGCTCAG TGTCCGACCT GCCCCTGCCC CTCCCCCAGC CCACCCTGGC CTCCCCTGGT 
TCACCCGAAC CTCTGCCCTT TCTGTCACTC AACGGCGGCG GCCCAGAGTT GGCCGGGGAT 
TGGGGTGGGG CTGGAGATGC CCCGCTGTCC CCAGACCCAC TGCTGCCCCC GCTCCCCAGC 
TCCCCGCAAG CAGCCGTCCC CTCTCCCCTC CCCACGGGGC CCCCAGAGAG GCCAGCACAC 
TGTGGGCTCC CGCCTTCTCC CGGTGACATC CCGGCCTCTG CCTCCACCTG TGGCCCACCT 
GACCACCTGC TGCCTCCTCT GGGTGCCCCG CTGCCCCCGG GCTGGGTGAC CATGGAGGAA 
GACTTTGTGC TCATGTTGGC TCTTTCACCC AGCCACCTGG GTGCTGACCT GGTGGCAGTC 
CCTCATGCTC GCTTTGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGGGG CGGCATCTCG 
AGGGCCACCC TGTTGCGCCT TTTCTTGGCT ATGGAGCATG GTACCCACTT CGGCCTGGGG 
TGTCCCCAGC TGGGCTATGC CACAGCCCAT GCTTTCCGCC TTGAGCCGCT CACGCCTCGC 
GGCGTGCTCA CGGTGGATGG CGAGCAGGTA GAGTACGGCC CGATGCAGGC ACAGGTGCAC 
CCGGGCCTTG GTACGCTCCT CACTGGGCCG CCTGGCCGGG TGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007956305.1
CDS	110..1957
Translation	MAPPLPQLAASTPLLHGEFGSYPARGPRFALTLTPQALYIQRLRPKPEARPRGGLVPLAEVSGCCTLRSRSPLDSAAYFCVYTYPQGRRGARRRATRTFRADGAATYEENRTEAHRWAVTLTCLLRGLPLPGDGDITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLMQTERPNHARELVQALSLSEWDGIITVSGDGLLYEVLNGLLDRPDWEEAVRMPVGILPCGSGNALAGAVNLHGGFAPALGLDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATGEPASPPPAHGLPRAKSELTLAPEPAPPVTHSPLHRSVSDLPLPLPQPTLASPGSPEPLPFLSLNGGGPELAGDWGGAGDAPLSPDPLLPPLPSSPQAAVPSPLPTGPPERPAHCGLPPSPGDIPASASTCGPPDHLLPPLGAPLPPGWVTMEEDFVLMLALSPSHLGADLVAVPHARFDDGLVHLCWVRGGISRATLLRLFLAMEHGTHFGLGCPQLGYATAHAFRLEPLTPRGVLTVDGEQVEYGPMQAQVHPGLGTLLTGPPGRVP

Target ORF information:

RefSeq Version	XM_007958114.1
Organism	Orycteropus afer afer
Definition	Orycteropus afer afer sphingosine kinase 2 (SPHK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007958114.1

ORF Insert Sequence:

ATGGCCCCGC CATTACCACA ACTGGCCGCC AGCACCCCAC TCCTGCACGG GGAGTTCGGC 
TCCTACCCGG CCCGTGGCCC CCGCTTCGCC CTCACGCTCA CGCCGCAGGC CCTGTACATC 
CAGCGACTGC GTCCCAAGCC TGAAGCCCGG CCCCGGGGCG GCCTGGTCCC GCTGGCCGAG 
GTCTCAGGCT GCTGCACCCT ACGCAGCCGC AGCCCACTGG ACTCTGCAGC CTACTTCTGC 
GTCTACACCT ACCCGCAGGG CCGGCGCGGG GCCCGGCGCA GAGCCACGCG CACCTTCCGG 
GCCGACGGGG CAGCCACCTA CGAGGAGAAC CGCACCGAGG CCCATCGCTG GGCGGTCACG 
CTCACATGCC TGCTCCGTGG GCTGCCACTG CCTGGGGATG GCGATATCAC CCCTGACCTG 
CTGCCCCGTC CGCCCCGTTT GCTTTTATTG GTCAACCCCT TTGGGGGGCG GGGCCTGGCC 
TGGCAGTGGT GTAAGAACCA CGTGTTGCCT ATGATCTCAG AAGCTGGGCT GTCCTTCAAC 
CTCATGCAGA CAGAGCGGCC AAACCACGCC CGGGAGCTGG TCCAGGCGCT GAGCCTGAGC 
GAGTGGGACG GCATCATCAC GGTCTCGGGA GACGGGCTGC TCTATGAGGT GCTGAACGGG 
CTCCTCGATC GCCCCGACTG GGAGGAGGCA GTGAGGATGC CCGTGGGCAT CCTCCCCTGC 
GGCTCGGGCA ACGCGCTGGC GGGCGCCGTG AACCTGCACG GGGGGTTTGC ACCAGCCCTG 
GGCCTCGACC TTCTGCTTAA CTGCTCGCTG CTGCTCTGCC GGGGTGGCAG CCACCCACTG 
GACCTGCTCT CTGTGACGCT GGCTTCCGGC TCCCGCTGTT TCTCCTTCCT GTCCGTGGCC 
TGGGGCTTCG TGTCCGACGT GGACATCCAG AGTGAGCGCT TCAGGGCCCT GGGCAGCGCC 
CGCTTCACTC TGGGCACAGT GCTGGGCCTC GCCACGCTGC ACACCTACCG AGGGCGGCTC 
TCCTACCTCC CGGCCACCGG GGAACCCGCC TCGCCCCCGC CCGCCCACGG CCTGCCCCGG 
GCCAAGTCGG AGCTGACCCT GGCCCCAGAG CCAGCCCCGC CCGTGACCCA CTCGCCTCTG 
CACCGCTCAG TGTCCGACCT GCCCCTGCCC CTCCCCCAGC CCACCCTGGC CTCCCCTGGT 
TCACCCGAAC CTCTGCCCTT TCTGTCACTC AACGGCGGCG GCCCAGAGTT GGCCGGGGAT 
TGGGGTGGGG CTGGAGATGC CCCGCTGTCC CCAGACCCAC TGCTGCCCCC GCTCCCCAGC 
TCCCCGCAAG CAGCCGTCCC CTCTCCCCTC CCCACGGGGC CCCCAGAGAG GCCAGCACAC 
TGTGGGCTCC CGCCTTCTCC CGGTGACATC CCGGCCTCTG CCTCCACCTG TGGCCCACCT 
GACCACCTGC TGCCTCCTCT GGGTGCCCCG CTGCCCCCGG GCTGGGTGAC CATGGAGGAA 
GACTTTGTGC TCATGTTGGC TCTTTCACCC AGCCACCTGG GTGCTGACCT GGTGGCAGTC 
CCTCATGCTC GCTTTGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGGGG CGGCATCTCG 
AGGGCCACCC TGTTGCGCCT TTTCTTGGCT ATGGAGCATG GTACCCACTT CGGCCTGGGG 
TGTCCCCAGC TGGGCTATGC CACAGCCCAT GCTTTCCGCC TTGAGCCGCT CACGCCTCGC 
GGCGTGCTCA CGGTGGATGG CGAGCAGGTA GAGTACGGCC CGATGCAGGC ACAGGTGCAC 
CCGGGCCTTG GTACGCTCCT CACTGGGCCG CCTGGCCGGG TGCCCTGA