LOC100708325 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100708325
Entrez Gene ID	100708325
Full Name	1-aminocyclopropane-1-carboxylate synthase-like protein 1
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005470975.3	XP_005471032.1	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
XM_003440502.5	XP_003440550.2	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X1
XM_013268686.2	XP_013124140.1	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
XM_013268685.3	XP_013124139.1	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
XM_013268685.2	XP_013124139.1	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
XM_005470975.4	XP_005471032.1	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
XM_003440502.4	XP_003440550.2	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100708325 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100708325 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr69673	XM_003440502.5 Latest version!	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOr69674	XM_013268686.2 Latest version!	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOr69674	XM_005470975.4 Latest version!	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOr69674	XM_013268685.3 Latest version!	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OOr69674	XM_013268685.2	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOr69674	XM_005470975.3	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOr69673	XM_003440502.4	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100708325 ( XM_003440502.5 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003440550.2 Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr69673
Clone ID Related Accession (Same CDS sequence)	XM_003440502.5 , XM_003440502.4
Accession Version	XM_003440502.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_003440502.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTGA CAGAAATGAG TGTTCTCATT TCGGAGGGCT GCATTTTTGA TTTAAGGAGG 
CATGAGCGAG GCAGCAACTG GACTGACCCG GAGATTGTGG AGCTGCTCCA GCTGTGGTCC 
GACGAGTCGG TGCAGATTGA ACTGGAGAGT TCACTGCGCA ACCAGCGCGT GTTTGACCGC 
ATCGCCCACA TTTTGCGTGA AAAGGGCATC TACCGCACAG GAGACCAGTG CAGGGAGAAG 
ATTAAAAAGA TGAAGCTGGA GTACCGCCGC ATCAAGGACA ACCACAAAAT GAGGTCTTGG 
AAGTTTTATG ATGTGATGGA CAGAGTGCTG GCGAATCGGC CGGCTATTAC CTACTCCTCC 
CTAGGCGGAG CTGTCATAGC TCAACAGGTG TTCCAGAGCT CTGGGGCACC TGAGGCATAC 
CTGCAAGGAG TGCCAGCGGG CTCTTTTGGT CCTGCTTCCT CCGGTGGGTT TCTCTTTGGC 
CAGCCTCCAA AAGCTGGAGA TCCGCTGGAT ATCAAATGTG AAGATGTGGA GGAGAGCATG 
CTGAACTCCG GAGAGGCACC GCCTGAGATG TACTACGGTT CTGGAGATGA TCAGGAAACT 
GATGGGCAGT CTCTGCTGGA GCCAGAAGAC ACCCTGGGCC AAGCCGAGAG CACACTTAAT 
ACAAGAATTT CACCTTCAGG TTTTAGTGAC CTGAACATGA CTGGCTCTGC TACAGCCACC 
ACCTTGGCTG CTGGTGGTCC CGTGTCACAC GACACATCCG ACGAACCCAG AAAGGAGGGT 
TCTCGTCAAC AGACCTCAGT GAGACAGAGG AAGCGGCGCC GGGGTGGTAA AACATCATGG 
TGCCATGGGG GCGGTAGATG TGGTGGTCAG GAGAGACTGG ATAAAGCCCT AGCCAGCTTC 
TTGAGCTGGC AGCAGTCTGC AGAGGAGCGT CTCCTCTCAC TGGAGGAGGC GCGGCTGGAG 
AGAGAGCTGC AGGCGGAGGA GCGCAGGGAG CAGCGGGAGG AGAGGAGGGC AGAACAAGAG 
CGTCAGCATG AGCTCCGCCT GTTCAGCATG CTCACGGGGG TGTTGCTCGC TGTCAGAGAG 
GGTACACAAA CCACAACACA AAGCGACCCC GCCACCTCAC CCTTAGCTCG TTTTTCAGCT 
TCGCTGATGA CAACGCCTGC CCCCTCTGCT CCTTCATCTT CATCCCAGCC ACCTCCAGAA 
GCTCCCACGA CAGAGTCTAT TTTTAATCAG GAGACAAAAA AGTCACAGCT CACATCTGTC 
AAGGCCTGCA AAATGTCTCA GAATGCTTTG GAAACACTGA GGGGTGAGGA AGGTCCCGGC 
AATAGCGTCT ACCTGTCTGA GCGTGGTAAT AGCATCCGAC TCCATGAAGG TATTCTCCAG 
GAGGGCTACA TCCAATATGG AATGGACCGG CACCATGATA CAGACAACCC TGATGGTATC 
ATCAACATGG GGACCAGTGA GAACAAACTT TGTTATGATC TTCTTCATAA ACGGCTGACC 
AAGCCTGACA TGCTGCACAT CAACCCATCC TTGTTGCAGT ATTCAGACTG GAGGGGGCAC 
GCTTTCCTCA GGGAGGCAGT TGCAAAATTC CTGACTCACT ACTGTTGTTC TCCAAACCCA 
TTAAAAGCTG ACAACGTTGT GGTGATGAAT GGCTGTGGTT CCCTGTTCTC ATGTATTGCT 
GCAGTCATTT GTGATCCTAA AGATGCCATC CTTATTCCGA CTCCTTTCTA CGGCGTCATC 
ACTGAAGATC TTCATCTGTA TAGTGATGTA AAACTCTTCC ACGTTCCCCT TGATTGTGAG 
GCTGATGGCA AAGATAACCG ACCCTTCCGC CTCACTGTAG GGAAACTAGA AGAAGGTCTG 
CAAAGAGCTA AGGAAGAGGG GTTGACAATT CGCGGCGTTA TCCTGATGAA CCCCCACAAC 
CCTCTGGCTG AAATCTACAG CCTGAAGGAA ATGATTACCT TCCTGGAGTT TGCCAAAAGA 
AATGCTCTCC ATGCCATTGT AGATGAAGTG TACATGCTGA CAGTGTTTGA TGAATCTGTC 
ACCTTTCAGA GTGTCCTCAG TGCAGACAGT TTGCCCGACC CACAGAGGAC GCATGTAATG 
TGGGGAATGA GCAAGGACTT TGCAATGGCA GGAATCAGAA TAGGCACTTT ATATACTGAG 
AACAGAGACC TCGTGGAGGC TTTGGCCAAA TTGGGCTCTT TTCATGGTGT CCCTGGGACT 
ACCCAGCACC AGGTTGCACA ACTGCTTCAG GACAAAGAGT GGATCAATGA GGAATTTTTG 
CCTGAGAACA GACGCAGGCT GAAAGCTGCG TGCAGCTACA TGACTGGAGA GCTGCTCAGT 
ATGGGCATCC CGTTTCTAGA CAGGCCTGCG ACACTCTATG TCTGGGCTGA CCTTAGAAAG 
TATCTCCAAG AGTCATCGTT TGAGGAGGAG CTGTCTCTGT GGAAGTGTTT CCTCAGGCAC 
AAGGTGCTGC TGAGTTGTGG CCAAGCGTTC TGCTGTTCAG TGCCCGGGTG GTTCCGCATC 
GTCTTTGCAG ACCAACAGCA CCACCTTCAG CTTGGCCTTA AGCGAATTAG AGAGGCTTTA 
AAAGAAACTG AAGAAAAAAT GAGAGTCCCG GACATCAAAC AAACCAGTGA GGAGAGCCAA 
AAGCCAGTAA AAAAGGACAA TGCAGGTTCA GACAATGCTG CTACTGTCAA TCCAAAAGCC 
TTAGCCGAGA ACAATTCATC AGATCAGCTG AAGGAGAGGG ATAGCCCTGT TCCTGACACA 
GGCTCACTGG CCACTGAAGA CTGCCAAGCC TCTAAGCCCG CAGAGAGTCT TGACACGCTG 
ATCGGTACTC TCAGGCATCA GATCCGTTCC TCCGATTGGC TGGAGAAAAG CACTCCAGAG 
CTGTCTGCTG GGGAGGACCC GGAAATTTTT GATGTGTTCA AGGTCCTGCT GCAAAGAGCC 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003440550.2
CDS	402..3350
Translation	MRVTEMSVLISEGCIFDLRRHERGSNWTDPEIVELLQLWSDESVQIELESSLRNQRVFDRIAHILREKGIYRTGDQCREKIKKMKLEYRRIKDNHKMRSWKFYDVMDRVLANRPAITYSSLGGAVIAQQVFQSSGAPEAYLQGVPAGSFGPASSGGFLFGQPPKAGDPLDIKCEDVEESMLNSGEAPPEMYYGSGDDQETDGQSLLEPEDTLGQAESTLNTRISPSGFSDLNMTGSATATTLAAGGPVSHDTSDEPRKEGSRQQTSVRQRKRRRGGKTSWCHGGGRCGGQERLDKALASFLSWQQSAEERLLSLEEARLERELQAEERREQREERRAEQERQHELRLFSMLTGVLLAVREGTQTTTQSDPATSPLARFSASLMTTPAPSAPSSSSQPPPEAPTTESIFNQETKKSQLTSVKACKMSQNALETLRGEEGPGNSVYLSERGNSIRLHEGILQEGYIQYGMDRHHDTDNPDGIINMGTSENKLCYDLLHKRLTKPDMLHINPSLLQYSDWRGHAFLREAVAKFLTHYCCSPNPLKADNVVVMNGCGSLFSCIAAVICDPKDAILIPTPFYGVITEDLHLYSDVKLFHVPLDCEADGKDNRPFRLTVGKLEEGLQRAKEEGLTIRGVILMNPHNPLAEIYSLKEMITFLEFAKRNALHAIVDEVYMLTVFDESVTFQSVLSADSLPDPQRTHVMWGMSKDFAMAGIRIGTLYTENRDLVEALAKLGSFHGVPGTTQHQVAQLLQDKEWINEEFLPENRRRLKAACSYMTGELLSMGIPFLDRPATLYVWADLRKYLQESSFEEELSLWKCFLRHKVLLSCGQAFCCSVPGWFRIVFADQQHHLQLGLKRIREALKETEEKMRVPDIKQTSEESQKPVKKDNAGSDNAATVNPKALAENNSSDQLKERDSPVPDTGSLATEDCQASKPAESLDTLIGTLRHQIRSSDWLEKSTPELSAGEDPEIFDVFKVLLQRARK

Target ORF information:

RefSeq Version	XM_003440502.5
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003440502.5

ORF Insert Sequence:

ATGAGAGTGA CAGAAATGAG TGTTCTCATT TCGGAGGGCT GCATTTTTGA TTTAAGGAGG 
CATGAGCGAG GCAGCAACTG GACTGACCCG GAGATTGTGG AGCTGCTCCA GCTGTGGTCC 
GACGAGTCGG TGCAGATTGA ACTGGAGAGT TCACTGCGCA ACCAGCGCGT GTTTGACCGC 
ATCGCCCACA TTTTGCGTGA AAAGGGCATC TACCGCACAG GAGACCAGTG CAGGGAGAAG 
ATTAAAAAGA TGAAGCTGGA GTACCGCCGC ATCAAGGACA ACCACAAAAT GAGGTCTTGG 
AAGTTTTATG ATGTGATGGA CAGAGTGCTG GCGAATCGGC CGGCTATTAC CTACTCCTCC 
CTAGGCGGAG CTGTCATAGC TCAACAGGTG TTCCAGAGCT CTGGGGCACC TGAGGCATAC 
CTGCAAGGAG TGCCAGCGGG CTCTTTTGGT CCTGCTTCCT CCGGTGGGTT TCTCTTTGGC 
CAGCCTCCAA AAGCTGGAGA TCCGCTGGAT ATCAAATGTG AAGATGTGGA GGAGAGCATG 
CTGAACTCCG GAGAGGCACC GCCTGAGATG TACTACGGTT CTGGAGATGA TCAGGAAACT 
GATGGGCAGT CTCTGCTGGA GCCAGAAGAC ACCCTGGGCC AAGCCGAGAG CACACTTAAT 
ACAAGAATTT CACCTTCAGG TTTTAGTGAC CTGAACATGA CTGGCTCTGC TACAGCCACC 
ACCTTGGCTG CTGGTGGTCC CGTGTCACAC GACACATCCG ACGAACCCAG AAAGGAGGGT 
TCTCGTCAAC AGACCTCAGT GAGACAGAGG AAGCGGCGCC GGGGTGGTAA AACATCATGG 
TGCCATGGGG GCGGTAGATG TGGTGGTCAG GAGAGACTGG ATAAAGCCCT AGCCAGCTTC 
TTGAGCTGGC AGCAGTCTGC AGAGGAGCGT CTCCTCTCAC TGGAGGAGGC GCGGCTGGAG 
AGAGAGCTGC AGGCGGAGGA GCGCAGGGAG CAGCGGGAGG AGAGGAGGGC AGAACAAGAG 
CGTCAGCATG AGCTCCGCCT GTTCAGCATG CTCACGGGGG TGTTGCTCGC TGTCAGAGAG 
GGTACACAAA CCACAACACA AAGCGACCCC GCCACCTCAC CCTTAGCTCG TTTTTCAGCT 
TCGCTGATGA CAACGCCTGC CCCCTCTGCT CCTTCATCTT CATCCCAGCC ACCTCCAGAA 
GCTCCCACGA CAGAGTCTAT TTTTAATCAG GAGACAAAAA AGTCACAGCT CACATCTGTC 
AAGGCCTGCA AAATGTCTCA GAATGCTTTG GAAACACTGA GGGGTGAGGA AGGTCCCGGC 
AATAGCGTCT ACCTGTCTGA GCGTGGTAAT AGCATCCGAC TCCATGAAGG TATTCTCCAG 
GAGGGCTACA TCCAATATGG AATGGACCGG CACCATGATA CAGACAACCC TGATGGTATC 
ATCAACATGG GGACCAGTGA GAACAAACTT TGTTATGATC TTCTTCATAA ACGGCTGACC 
AAGCCTGACA TGCTGCACAT CAACCCATCC TTGTTGCAGT ATTCAGACTG GAGGGGGCAC 
GCTTTCCTCA GGGAGGCAGT TGCAAAATTC CTGACTCACT ACTGTTGTTC TCCAAACCCA 
TTAAAAGCTG ACAACGTTGT GGTGATGAAT GGCTGTGGTT CCCTGTTCTC ATGTATTGCT 
GCAGTCATTT GTGATCCTAA AGATGCCATC CTTATTCCGA CTCCTTTCTA CGGCGTCATC 
ACTGAAGATC TTCATCTGTA TAGTGATGTA AAACTCTTCC ACGTTCCCCT TGATTGTGAG 
GCTGATGGCA AAGATAACCG ACCCTTCCGC CTCACTGTAG GGAAACTAGA AGAAGGTCTG 
CAAAGAGCTA AGGAAGAGGG GTTGACAATT CGCGGCGTTA TCCTGATGAA CCCCCACAAC 
CCTCTGGCTG AAATCTACAG CCTGAAGGAA ATGATTACCT TCCTGGAGTT TGCCAAAAGA 
AATGCTCTCC ATGCCATTGT AGATGAAGTG TACATGCTGA CAGTGTTTGA TGAATCTGTC 
ACCTTTCAGA GTGTCCTCAG TGCAGACAGT TTGCCCGACC CACAGAGGAC GCATGTAATG 
TGGGGAATGA GCAAGGACTT TGCAATGGCA GGAATCAGAA TAGGCACTTT ATATACTGAG 
AACAGAGACC TCGTGGAGGC TTTGGCCAAA TTGGGCTCTT TTCATGGTGT CCCTGGGACT 
ACCCAGCACC AGGTTGCACA ACTGCTTCAG GACAAAGAGT GGATCAATGA GGAATTTTTG 
CCTGAGAACA GACGCAGGCT GAAAGCTGCG TGCAGCTACA TGACTGGAGA GCTGCTCAGT 
ATGGGCATCC CGTTTCTAGA CAGGCCTGCG ACACTCTATG TCTGGGCTGA CCTTAGAAAG 
TATCTCCAAG AGTCATCGTT TGAGGAGGAG CTGTCTCTGT GGAAGTGTTT CCTCAGGCAC 
AAGGTGCTGC TGAGTTGTGG CCAAGCGTTC TGCTGTTCAG TGCCCGGGTG GTTCCGCATC 
GTCTTTGCAG ACCAACAGCA CCACCTTCAG CTTGGCCTTA AGCGAATTAG AGAGGCTTTA 
AAAGAAACTG AAGAAAAAAT GAGAGTCCCG GACATCAAAC AAACCAGTGA GGAGAGCCAA 
AAGCCAGTAA AAAAGGACAA TGCAGGTTCA GACAATGCTG CTACTGTCAA TCCAAAAGCC 
TTAGCCGAGA ACAATTCATC AGATCAGCTG AAGGAGAGGG ATAGCCCTGT TCCTGACACA 
GGCTCACTGG CCACTGAAGA CTGCCAAGCC TCTAAGCCCG CAGAGAGTCT TGACACGCTG 
ATCGGTACTC TCAGGCATCA GATCCGTTCC TCCGATTGGC TGGAGAAAAG CACTCCAGAG 
CTGTCTGCTG GGGAGGACCC GGAAATTTTT GATGTGTTCA AGGTCCTGCT GCAAAGAGCC 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100708325 ( XM_003440502.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003440550.2 Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr69673
Clone ID Related Accession (Same CDS sequence)	XM_003440502.5 , XM_003440502.4
Accession Version	XM_003440502.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_003440502.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTGA CAGAAATGAG TGTTCTCATT TCGGAGGGCT GCATTTTTGA TTTAAGGAGG 
CATGAGCGAG GCAGCAACTG GACTGACCCG GAGATTGTGG AGCTGCTCCA GCTGTGGTCC 
GACGAGTCGG TGCAGATTGA ACTGGAGAGT TCACTGCGCA ACCAGCGCGT GTTTGACCGC 
ATCGCCCACA TTTTGCGTGA AAAGGGCATC TACCGCACAG GAGACCAGTG CAGGGAGAAG 
ATTAAAAAGA TGAAGCTGGA GTACCGCCGC ATCAAGGACA ACCACAAAAT GAGGTCTTGG 
AAGTTTTATG ATGTGATGGA CAGAGTGCTG GCGAATCGGC CGGCTATTAC CTACTCCTCC 
CTAGGCGGAG CTGTCATAGC TCAACAGGTG TTCCAGAGCT CTGGGGCACC TGAGGCATAC 
CTGCAAGGAG TGCCAGCGGG CTCTTTTGGT CCTGCTTCCT CCGGTGGGTT TCTCTTTGGC 
CAGCCTCCAA AAGCTGGAGA TCCGCTGGAT ATCAAATGTG AAGATGTGGA GGAGAGCATG 
CTGAACTCCG GAGAGGCACC GCCTGAGATG TACTACGGTT CTGGAGATGA TCAGGAAACT 
GATGGGCAGT CTCTGCTGGA GCCAGAAGAC ACCCTGGGCC AAGCCGAGAG CACACTTAAT 
ACAAGAATTT CACCTTCAGG TTTTAGTGAC CTGAACATGA CTGGCTCTGC TACAGCCACC 
ACCTTGGCTG CTGGTGGTCC CGTGTCACAC GACACATCCG ACGAACCCAG AAAGGAGGGT 
TCTCGTCAAC AGACCTCAGT GAGACAGAGG AAGCGGCGCC GGGGTGGTAA AACATCATGG 
TGCCATGGGG GCGGTAGATG TGGTGGTCAG GAGAGACTGG ATAAAGCCCT AGCCAGCTTC 
TTGAGCTGGC AGCAGTCTGC AGAGGAGCGT CTCCTCTCAC TGGAGGAGGC GCGGCTGGAG 
AGAGAGCTGC AGGCGGAGGA GCGCAGGGAG CAGCGGGAGG AGAGGAGGGC AGAACAAGAG 
CGTCAGCATG AGCTCCGCCT GTTCAGCATG CTCACGGGGG TGTTGCTCGC TGTCAGAGAG 
GGTACACAAA CCACAACACA AAGCGACCCC GCCACCTCAC CCTTAGCTCG TTTTTCAGCT 
TCGCTGATGA CAACGCCTGC CCCCTCTGCT CCTTCATCTT CATCCCAGCC ACCTCCAGAA 
GCTCCCACGA CAGAGTCTAT TTTTAATCAG GAGACAAAAA AGTCACAGCT CACATCTGTC 
AAGGCCTGCA AAATGTCTCA GAATGCTTTG GAAACACTGA GGGGTGAGGA AGGTCCCGGC 
AATAGCGTCT ACCTGTCTGA GCGTGGTAAT AGCATCCGAC TCCATGAAGG TATTCTCCAG 
GAGGGCTACA TCCAATATGG AATGGACCGG CACCATGATA CAGACAACCC TGATGGTATC 
ATCAACATGG GGACCAGTGA GAACAAACTT TGTTATGATC TTCTTCATAA ACGGCTGACC 
AAGCCTGACA TGCTGCACAT CAACCCATCC TTGTTGCAGT ATTCAGACTG GAGGGGGCAC 
GCTTTCCTCA GGGAGGCAGT TGCAAAATTC CTGACTCACT ACTGTTGTTC TCCAAACCCA 
TTAAAAGCTG ACAACGTTGT GGTGATGAAT GGCTGTGGTT CCCTGTTCTC ATGTATTGCT 
GCAGTCATTT GTGATCCTAA AGATGCCATC CTTATTCCGA CTCCTTTCTA CGGCGTCATC 
ACTGAAGATC TTCATCTGTA TAGTGATGTA AAACTCTTCC ACGTTCCCCT TGATTGTGAG 
GCTGATGGCA AAGATAACCG ACCCTTCCGC CTCACTGTAG GGAAACTAGA AGAAGGTCTG 
CAAAGAGCTA AGGAAGAGGG GTTGACAATT CGCGGCGTTA TCCTGATGAA CCCCCACAAC 
CCTCTGGCTG AAATCTACAG CCTGAAGGAA ATGATTACCT TCCTGGAGTT TGCCAAAAGA 
AATGCTCTCC ATGCCATTGT AGATGAAGTG TACATGCTGA CAGTGTTTGA TGAATCTGTC 
ACCTTTCAGA GTGTCCTCAG TGCAGACAGT TTGCCCGACC CACAGAGGAC GCATGTAATG 
TGGGGAATGA GCAAGGACTT TGCAATGGCA GGAATCAGAA TAGGCACTTT ATATACTGAG 
AACAGAGACC TCGTGGAGGC TTTGGCCAAA TTGGGCTCTT TTCATGGTGT CCCTGGGACT 
ACCCAGCACC AGGTTGCACA ACTGCTTCAG GACAAAGAGT GGATCAATGA GGAATTTTTG 
CCTGAGAACA GACGCAGGCT GAAAGCTGCG TGCAGCTACA TGACTGGAGA GCTGCTCAGT 
ATGGGCATCC CGTTTCTAGA CAGGCCTGCG ACACTCTATG TCTGGGCTGA CCTTAGAAAG 
TATCTCCAAG AGTCATCGTT TGAGGAGGAG CTGTCTCTGT GGAAGTGTTT CCTCAGGCAC 
AAGGTGCTGC TGAGTTGTGG CCAAGCGTTC TGCTGTTCAG TGCCCGGGTG GTTCCGCATC 
GTCTTTGCAG ACCAACAGCA CCACCTTCAG CTTGGCCTTA AGCGAATTAG AGAGGCTTTA 
AAAGAAACTG AAGAAAAAAT GAGAGTCCCG GACATCAAAC AAACCAGTGA GGAGAGCCAA 
AAGCCAGTAA AAAAGGACAA TGCAGGTTCA GACAATGCTG CTACTGTCAA TCCAAAAGCC 
TTAGCCGAGA ACAATTCATC AGATCAGCTG AAGGAGAGGG ATAGCCCTGT TCCTGACACA 
GGCTCACTGG CCACTGAAGA CTGCCAAGCC TCTAAGCCCG CAGAGAGTCT TGACACGCTG 
ATCGGTACTC TCAGGCATCA GATCCGTTCC TCCGATTGGC TGGAGAAAAG CACTCCAGAG 
CTGTCTGCTG GGGAGGACCC GGAAATTTTT GATGTGTTCA AGGTCCTGCT GCAAAGAGCC 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003440550.2
CDS	433..3381
Translation	MRVTEMSVLISEGCIFDLRRHERGSNWTDPEIVELLQLWSDESVQIELESSLRNQRVFDRIAHILREKGIYRTGDQCREKIKKMKLEYRRIKDNHKMRSWKFYDVMDRVLANRPAITYSSLGGAVIAQQVFQSSGAPEAYLQGVPAGSFGPASSGGFLFGQPPKAGDPLDIKCEDVEESMLNSGEAPPEMYYGSGDDQETDGQSLLEPEDTLGQAESTLNTRISPSGFSDLNMTGSATATTLAAGGPVSHDTSDEPRKEGSRQQTSVRQRKRRRGGKTSWCHGGGRCGGQERLDKALASFLSWQQSAEERLLSLEEARLERELQAEERREQREERRAEQERQHELRLFSMLTGVLLAVREGTQTTTQSDPATSPLARFSASLMTTPAPSAPSSSSQPPPEAPTTESIFNQETKKSQLTSVKACKMSQNALETLRGEEGPGNSVYLSERGNSIRLHEGILQEGYIQYGMDRHHDTDNPDGIINMGTSENKLCYDLLHKRLTKPDMLHINPSLLQYSDWRGHAFLREAVAKFLTHYCCSPNPLKADNVVVMNGCGSLFSCIAAVICDPKDAILIPTPFYGVITEDLHLYSDVKLFHVPLDCEADGKDNRPFRLTVGKLEEGLQRAKEEGLTIRGVILMNPHNPLAEIYSLKEMITFLEFAKRNALHAIVDEVYMLTVFDESVTFQSVLSADSLPDPQRTHVMWGMSKDFAMAGIRIGTLYTENRDLVEALAKLGSFHGVPGTTQHQVAQLLQDKEWINEEFLPENRRRLKAACSYMTGELLSMGIPFLDRPATLYVWADLRKYLQESSFEEELSLWKCFLRHKVLLSCGQAFCCSVPGWFRIVFADQQHHLQLGLKRIREALKETEEKMRVPDIKQTSEESQKPVKKDNAGSDNAATVNPKALAENNSSDQLKERDSPVPDTGSLATEDCQASKPAESLDTLIGTLRHQIRSSDWLEKSTPELSAGEDPEIFDVFKVLLQRARK

Target ORF information:

RefSeq Version	XM_003440502.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003440502.4

ORF Insert Sequence:

ATGAGAGTGA CAGAAATGAG TGTTCTCATT TCGGAGGGCT GCATTTTTGA TTTAAGGAGG 
CATGAGCGAG GCAGCAACTG GACTGACCCG GAGATTGTGG AGCTGCTCCA GCTGTGGTCC 
GACGAGTCGG TGCAGATTGA ACTGGAGAGT TCACTGCGCA ACCAGCGCGT GTTTGACCGC 
ATCGCCCACA TTTTGCGTGA AAAGGGCATC TACCGCACAG GAGACCAGTG CAGGGAGAAG 
ATTAAAAAGA TGAAGCTGGA GTACCGCCGC ATCAAGGACA ACCACAAAAT GAGGTCTTGG 
AAGTTTTATG ATGTGATGGA CAGAGTGCTG GCGAATCGGC CGGCTATTAC CTACTCCTCC 
CTAGGCGGAG CTGTCATAGC TCAACAGGTG TTCCAGAGCT CTGGGGCACC TGAGGCATAC 
CTGCAAGGAG TGCCAGCGGG CTCTTTTGGT CCTGCTTCCT CCGGTGGGTT TCTCTTTGGC 
CAGCCTCCAA AAGCTGGAGA TCCGCTGGAT ATCAAATGTG AAGATGTGGA GGAGAGCATG 
CTGAACTCCG GAGAGGCACC GCCTGAGATG TACTACGGTT CTGGAGATGA TCAGGAAACT 
GATGGGCAGT CTCTGCTGGA GCCAGAAGAC ACCCTGGGCC AAGCCGAGAG CACACTTAAT 
ACAAGAATTT CACCTTCAGG TTTTAGTGAC CTGAACATGA CTGGCTCTGC TACAGCCACC 
ACCTTGGCTG CTGGTGGTCC CGTGTCACAC GACACATCCG ACGAACCCAG AAAGGAGGGT 
TCTCGTCAAC AGACCTCAGT GAGACAGAGG AAGCGGCGCC GGGGTGGTAA AACATCATGG 
TGCCATGGGG GCGGTAGATG TGGTGGTCAG GAGAGACTGG ATAAAGCCCT AGCCAGCTTC 
TTGAGCTGGC AGCAGTCTGC AGAGGAGCGT CTCCTCTCAC TGGAGGAGGC GCGGCTGGAG 
AGAGAGCTGC AGGCGGAGGA GCGCAGGGAG CAGCGGGAGG AGAGGAGGGC AGAACAAGAG 
CGTCAGCATG AGCTCCGCCT GTTCAGCATG CTCACGGGGG TGTTGCTCGC TGTCAGAGAG 
GGTACACAAA CCACAACACA AAGCGACCCC GCCACCTCAC CCTTAGCTCG TTTTTCAGCT 
TCGCTGATGA CAACGCCTGC CCCCTCTGCT CCTTCATCTT CATCCCAGCC ACCTCCAGAA 
GCTCCCACGA CAGAGTCTAT TTTTAATCAG GAGACAAAAA AGTCACAGCT CACATCTGTC 
AAGGCCTGCA AAATGTCTCA GAATGCTTTG GAAACACTGA GGGGTGAGGA AGGTCCCGGC 
AATAGCGTCT ACCTGTCTGA GCGTGGTAAT AGCATCCGAC TCCATGAAGG TATTCTCCAG 
GAGGGCTACA TCCAATATGG AATGGACCGG CACCATGATA CAGACAACCC TGATGGTATC 
ATCAACATGG GGACCAGTGA GAACAAACTT TGTTATGATC TTCTTCATAA ACGGCTGACC 
AAGCCTGACA TGCTGCACAT CAACCCATCC TTGTTGCAGT ATTCAGACTG GAGGGGGCAC 
GCTTTCCTCA GGGAGGCAGT TGCAAAATTC CTGACTCACT ACTGTTGTTC TCCAAACCCA 
TTAAAAGCTG ACAACGTTGT GGTGATGAAT GGCTGTGGTT CCCTGTTCTC ATGTATTGCT 
GCAGTCATTT GTGATCCTAA AGATGCCATC CTTATTCCGA CTCCTTTCTA CGGCGTCATC 
ACTGAAGATC TTCATCTGTA TAGTGATGTA AAACTCTTCC ACGTTCCCCT TGATTGTGAG 
GCTGATGGCA AAGATAACCG ACCCTTCCGC CTCACTGTAG GGAAACTAGA AGAAGGTCTG 
CAAAGAGCTA AGGAAGAGGG GTTGACAATT CGCGGCGTTA TCCTGATGAA CCCCCACAAC 
CCTCTGGCTG AAATCTACAG CCTGAAGGAA ATGATTACCT TCCTGGAGTT TGCCAAAAGA 
AATGCTCTCC ATGCCATTGT AGATGAAGTG TACATGCTGA CAGTGTTTGA TGAATCTGTC 
ACCTTTCAGA GTGTCCTCAG TGCAGACAGT TTGCCCGACC CACAGAGGAC GCATGTAATG 
TGGGGAATGA GCAAGGACTT TGCAATGGCA GGAATCAGAA TAGGCACTTT ATATACTGAG 
AACAGAGACC TCGTGGAGGC TTTGGCCAAA TTGGGCTCTT TTCATGGTGT CCCTGGGACT 
ACCCAGCACC AGGTTGCACA ACTGCTTCAG GACAAAGAGT GGATCAATGA GGAATTTTTG 
CCTGAGAACA GACGCAGGCT GAAAGCTGCG TGCAGCTACA TGACTGGAGA GCTGCTCAGT 
ATGGGCATCC CGTTTCTAGA CAGGCCTGCG ACACTCTATG TCTGGGCTGA CCTTAGAAAG 
TATCTCCAAG AGTCATCGTT TGAGGAGGAG CTGTCTCTGT GGAAGTGTTT CCTCAGGCAC 
AAGGTGCTGC TGAGTTGTGG CCAAGCGTTC TGCTGTTCAG TGCCCGGGTG GTTCCGCATC 
GTCTTTGCAG ACCAACAGCA CCACCTTCAG CTTGGCCTTA AGCGAATTAG AGAGGCTTTA 
AAAGAAACTG AAGAAAAAAT GAGAGTCCCG GACATCAAAC AAACCAGTGA GGAGAGCCAA 
AAGCCAGTAA AAAAGGACAA TGCAGGTTCA GACAATGCTG CTACTGTCAA TCCAAAAGCC 
TTAGCCGAGA ACAATTCATC AGATCAGCTG AAGGAGAGGG ATAGCCCTGT TCCTGACACA 
GGCTCACTGG CCACTGAAGA CTGCCAAGCC TCTAAGCCCG CAGAGAGTCT TGACACGCTG 
ATCGGTACTC TCAGGCATCA GATCCGTTCC TCCGATTGGC TGGAGAAAAG CACTCCAGAG 
CTGTCTGCTG GGGAGGACCC GGAAATTTTT GATGTGTTCA AGGTCCTGCT GCAAAGAGCC 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100708325 ( XM_005470975.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005471032.1 Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOr69674
Clone ID Related Accession (Same CDS sequence)	XM_005470975.3 , XM_013268686.2 , XM_013268685.3 , XM_013268685.2 , XM_005470975.4
Accession Version	XM_005470975.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_005470975.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005471032.1
CDS	564..3473
Translation	MDFRGRRHERGSNWTDPEIVELLQLWSDESVQIELESSLRNQRVFDRIAHILREKGIYRTGDQCREKIKKMKLEYRRIKDNHKMRSWKFYDVMDRVLANRPAITYSSLGGAVIAQQVFQSSGAPEAYLQGVPAGSFGPASSGGFLFGQPPKAGDPLDIKCEDVEESMLNSGEAPPEMYYGSGDDQETDGQSLLEPEDTLGQAESTLNTRISPSGFSDLNMTGSATATTLAAGGPVSHDTSDEPRKEGSRQQTSVRQRKRRRGGKTSWCHGGGRCGGQERLDKALASFLSWQQSAEERLLSLEEARLERELQAEERREQREERRAEQERQHELRLFSMLTGVLLAVREGTQTTTQSDPATSPLARFSASLMTTPAPSAPSSSSQPPPEAPTTESIFNQETKKSQLTSVKACKMSQNALETLRGEEGPGNSVYLSERGNSIRLHEGILQEGYIQYGMDRHHDTDNPDGIINMGTSENKLCYDLLHKRLTKPDMLHINPSLLQYSDWRGHAFLREAVAKFLTHYCCSPNPLKADNVVVMNGCGSLFSCIAAVICDPKDAILIPTPFYGVITEDLHLYSDVKLFHVPLDCEADGKDNRPFRLTVGKLEEGLQRAKEEGLTIRGVILMNPHNPLAEIYSLKEMITFLEFAKRNALHAIVDEVYMLTVFDESVTFQSVLSADSLPDPQRTHVMWGMSKDFAMAGIRIGTLYTENRDLVEALAKLGSFHGVPGTTQHQVAQLLQDKEWINEEFLPENRRRLKAACSYMTGELLSMGIPFLDRPATLYVWADLRKYLQESSFEEELSLWKCFLRHKVLLSCGQAFCCSVPGWFRIVFADQQHHLQLGLKRIREALKETEEKMRVPDIKQTSEESQKPVKKDNAGSDNAATVNPKALAENNSSDQLKERDSPVPDTGSLATEDCQASKPAESLDTLIGTLRHQIRSSDWLEKSTPELSAGEDPEIFDVFKVLLQRARK

Target ORF information:

RefSeq Version	XM_005470975.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005470975.3

ORF Insert Sequence:

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100708325 ( XM_013268686.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013124140.1 Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOr69674
Clone ID Related Accession (Same CDS sequence)	XM_005470975.3 , XM_013268686.2 , XM_013268685.3 , XM_013268685.2 , XM_005470975.4
Accession Version	XM_013268686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_013268686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013124140.1
CDS	510..3419
Translation	MDFRGRRHERGSNWTDPEIVELLQLWSDESVQIELESSLRNQRVFDRIAHILREKGIYRTGDQCREKIKKMKLEYRRIKDNHKMRSWKFYDVMDRVLANRPAITYSSLGGAVIAQQVFQSSGAPEAYLQGVPAGSFGPASSGGFLFGQPPKAGDPLDIKCEDVEESMLNSGEAPPEMYYGSGDDQETDGQSLLEPEDTLGQAESTLNTRISPSGFSDLNMTGSATATTLAAGGPVSHDTSDEPRKEGSRQQTSVRQRKRRRGGKTSWCHGGGRCGGQERLDKALASFLSWQQSAEERLLSLEEARLERELQAEERREQREERRAEQERQHELRLFSMLTGVLLAVREGTQTTTQSDPATSPLARFSASLMTTPAPSAPSSSSQPPPEAPTTESIFNQETKKSQLTSVKACKMSQNALETLRGEEGPGNSVYLSERGNSIRLHEGILQEGYIQYGMDRHHDTDNPDGIINMGTSENKLCYDLLHKRLTKPDMLHINPSLLQYSDWRGHAFLREAVAKFLTHYCCSPNPLKADNVVVMNGCGSLFSCIAAVICDPKDAILIPTPFYGVITEDLHLYSDVKLFHVPLDCEADGKDNRPFRLTVGKLEEGLQRAKEEGLTIRGVILMNPHNPLAEIYSLKEMITFLEFAKRNALHAIVDEVYMLTVFDESVTFQSVLSADSLPDPQRTHVMWGMSKDFAMAGIRIGTLYTENRDLVEALAKLGSFHGVPGTTQHQVAQLLQDKEWINEEFLPENRRRLKAACSYMTGELLSMGIPFLDRPATLYVWADLRKYLQESSFEEELSLWKCFLRHKVLLSCGQAFCCSVPGWFRIVFADQQHHLQLGLKRIREALKETEEKMRVPDIKQTSEESQKPVKKDNAGSDNAATVNPKALAENNSSDQLKERDSPVPDTGSLATEDCQASKPAESLDTLIGTLRHQIRSSDWLEKSTPELSAGEDPEIFDVFKVLLQRARK

Target ORF information:

RefSeq Version	XM_013268686.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013268686.2

ORF Insert Sequence:

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100708325 ( XM_013268685.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013124139.1 Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOr69674
Clone ID Related Accession (Same CDS sequence)	XM_005470975.3 , XM_013268686.2 , XM_013268685.3 , XM_013268685.2 , XM_005470975.4
Accession Version	XM_013268685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_013268685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013124139.1
CDS	622..3531
Translation	MDFRGRRHERGSNWTDPEIVELLQLWSDESVQIELESSLRNQRVFDRIAHILREKGIYRTGDQCREKIKKMKLEYRRIKDNHKMRSWKFYDVMDRVLANRPAITYSSLGGAVIAQQVFQSSGAPEAYLQGVPAGSFGPASSGGFLFGQPPKAGDPLDIKCEDVEESMLNSGEAPPEMYYGSGDDQETDGQSLLEPEDTLGQAESTLNTRISPSGFSDLNMTGSATATTLAAGGPVSHDTSDEPRKEGSRQQTSVRQRKRRRGGKTSWCHGGGRCGGQERLDKALASFLSWQQSAEERLLSLEEARLERELQAEERREQREERRAEQERQHELRLFSMLTGVLLAVREGTQTTTQSDPATSPLARFSASLMTTPAPSAPSSSSQPPPEAPTTESIFNQETKKSQLTSVKACKMSQNALETLRGEEGPGNSVYLSERGNSIRLHEGILQEGYIQYGMDRHHDTDNPDGIINMGTSENKLCYDLLHKRLTKPDMLHINPSLLQYSDWRGHAFLREAVAKFLTHYCCSPNPLKADNVVVMNGCGSLFSCIAAVICDPKDAILIPTPFYGVITEDLHLYSDVKLFHVPLDCEADGKDNRPFRLTVGKLEEGLQRAKEEGLTIRGVILMNPHNPLAEIYSLKEMITFLEFAKRNALHAIVDEVYMLTVFDESVTFQSVLSADSLPDPQRTHVMWGMSKDFAMAGIRIGTLYTENRDLVEALAKLGSFHGVPGTTQHQVAQLLQDKEWINEEFLPENRRRLKAACSYMTGELLSMGIPFLDRPATLYVWADLRKYLQESSFEEELSLWKCFLRHKVLLSCGQAFCCSVPGWFRIVFADQQHHLQLGLKRIREALKETEEKMRVPDIKQTSEESQKPVKKDNAGSDNAATVNPKALAENNSSDQLKERDSPVPDTGSLATEDCQASKPAESLDTLIGTLRHQIRSSDWLEKSTPELSAGEDPEIFDVFKVLLQRARK

Target ORF information:

RefSeq Version	XM_013268685.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013268685.3

ORF Insert Sequence:

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100708325 ( XM_013268685.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013124139.1 Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOr69674
Clone ID Related Accession (Same CDS sequence)	XM_005470975.3 , XM_013268686.2 , XM_013268685.3 , XM_013268685.2 , XM_005470975.4
Accession Version	XM_013268685.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_013268685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013124139.1
CDS	654..3563
Translation	MDFRGRRHERGSNWTDPEIVELLQLWSDESVQIELESSLRNQRVFDRIAHILREKGIYRTGDQCREKIKKMKLEYRRIKDNHKMRSWKFYDVMDRVLANRPAITYSSLGGAVIAQQVFQSSGAPEAYLQGVPAGSFGPASSGGFLFGQPPKAGDPLDIKCEDVEESMLNSGEAPPEMYYGSGDDQETDGQSLLEPEDTLGQAESTLNTRISPSGFSDLNMTGSATATTLAAGGPVSHDTSDEPRKEGSRQQTSVRQRKRRRGGKTSWCHGGGRCGGQERLDKALASFLSWQQSAEERLLSLEEARLERELQAEERREQREERRAEQERQHELRLFSMLTGVLLAVREGTQTTTQSDPATSPLARFSASLMTTPAPSAPSSSSQPPPEAPTTESIFNQETKKSQLTSVKACKMSQNALETLRGEEGPGNSVYLSERGNSIRLHEGILQEGYIQYGMDRHHDTDNPDGIINMGTSENKLCYDLLHKRLTKPDMLHINPSLLQYSDWRGHAFLREAVAKFLTHYCCSPNPLKADNVVVMNGCGSLFSCIAAVICDPKDAILIPTPFYGVITEDLHLYSDVKLFHVPLDCEADGKDNRPFRLTVGKLEEGLQRAKEEGLTIRGVILMNPHNPLAEIYSLKEMITFLEFAKRNALHAIVDEVYMLTVFDESVTFQSVLSADSLPDPQRTHVMWGMSKDFAMAGIRIGTLYTENRDLVEALAKLGSFHGVPGTTQHQVAQLLQDKEWINEEFLPENRRRLKAACSYMTGELLSMGIPFLDRPATLYVWADLRKYLQESSFEEELSLWKCFLRHKVLLSCGQAFCCSVPGWFRIVFADQQHHLQLGLKRIREALKETEEKMRVPDIKQTSEESQKPVKKDNAGSDNAATVNPKALAENNSSDQLKERDSPVPDTGSLATEDCQASKPAESLDTLIGTLRHQIRSSDWLEKSTPELSAGEDPEIFDVFKVLLQRARK

Target ORF information:

RefSeq Version	XM_013268685.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013268685.2

ORF Insert Sequence:

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100708325 ( XM_005470975.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005471032.1 Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X3, mRNA.

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CloneID	OOr69674
Clone ID Related Accession (Same CDS sequence)	XM_005470975.3 , XM_013268686.2 , XM_013268685.3 , XM_013268685.2 , XM_005470975.4
Accession Version	XM_005470975.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	1-aminocyclopropane-1-carboxylate synthase-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_005470975.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005471032.1
CDS	532..3441
Translation	MDFRGRRHERGSNWTDPEIVELLQLWSDESVQIELESSLRNQRVFDRIAHILREKGIYRTGDQCREKIKKMKLEYRRIKDNHKMRSWKFYDVMDRVLANRPAITYSSLGGAVIAQQVFQSSGAPEAYLQGVPAGSFGPASSGGFLFGQPPKAGDPLDIKCEDVEESMLNSGEAPPEMYYGSGDDQETDGQSLLEPEDTLGQAESTLNTRISPSGFSDLNMTGSATATTLAAGGPVSHDTSDEPRKEGSRQQTSVRQRKRRRGGKTSWCHGGGRCGGQERLDKALASFLSWQQSAEERLLSLEEARLERELQAEERREQREERRAEQERQHELRLFSMLTGVLLAVREGTQTTTQSDPATSPLARFSASLMTTPAPSAPSSSSQPPPEAPTTESIFNQETKKSQLTSVKACKMSQNALETLRGEEGPGNSVYLSERGNSIRLHEGILQEGYIQYGMDRHHDTDNPDGIINMGTSENKLCYDLLHKRLTKPDMLHINPSLLQYSDWRGHAFLREAVAKFLTHYCCSPNPLKADNVVVMNGCGSLFSCIAAVICDPKDAILIPTPFYGVITEDLHLYSDVKLFHVPLDCEADGKDNRPFRLTVGKLEEGLQRAKEEGLTIRGVILMNPHNPLAEIYSLKEMITFLEFAKRNALHAIVDEVYMLTVFDESVTFQSVLSADSLPDPQRTHVMWGMSKDFAMAGIRIGTLYTENRDLVEALAKLGSFHGVPGTTQHQVAQLLQDKEWINEEFLPENRRRLKAACSYMTGELLSMGIPFLDRPATLYVWADLRKYLQESSFEEELSLWKCFLRHKVLLSCGQAFCCSVPGWFRIVFADQQHHLQLGLKRIREALKETEEKMRVPDIKQTSEESQKPVKKDNAGSDNAATVNPKALAENNSSDQLKERDSPVPDTGSLATEDCQASKPAESLDTLIGTLRHQIRSSDWLEKSTPELSAGEDPEIFDVFKVLLQRARK

Target ORF information:

RefSeq Version	XM_005470975.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus 1-aminocyclopropane-1-carboxylate synthase-like protein 1 (LOC100708325), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005470975.4

ORF Insert Sequence:

ATGGACTTCC GCGGCAGGAG GCATGAGCGA GGCAGCAACT GGACTGACCC GGAGATTGTG 
GAGCTGCTCC AGCTGTGGTC CGACGAGTCG GTGCAGATTG AACTGGAGAG TTCACTGCGC 
AACCAGCGCG TGTTTGACCG CATCGCCCAC ATTTTGCGTG AAAAGGGCAT CTACCGCACA 
GGAGACCAGT GCAGGGAGAA GATTAAAAAG ATGAAGCTGG AGTACCGCCG CATCAAGGAC 
AACCACAAAA TGAGGTCTTG GAAGTTTTAT GATGTGATGG ACAGAGTGCT GGCGAATCGG 
CCGGCTATTA CCTACTCCTC CCTAGGCGGA GCTGTCATAG CTCAACAGGT GTTCCAGAGC 
TCTGGGGCAC CTGAGGCATA CCTGCAAGGA GTGCCAGCGG GCTCTTTTGG TCCTGCTTCC 
TCCGGTGGGT TTCTCTTTGG CCAGCCTCCA AAAGCTGGAG ATCCGCTGGA TATCAAATGT 
GAAGATGTGG AGGAGAGCAT GCTGAACTCC GGAGAGGCAC CGCCTGAGAT GTACTACGGT 
TCTGGAGATG ATCAGGAAAC TGATGGGCAG TCTCTGCTGG AGCCAGAAGA CACCCTGGGC 
CAAGCCGAGA GCACACTTAA TACAAGAATT TCACCTTCAG GTTTTAGTGA CCTGAACATG 
ACTGGCTCTG CTACAGCCAC CACCTTGGCT GCTGGTGGTC CCGTGTCACA CGACACATCC 
GACGAACCCA GAAAGGAGGG TTCTCGTCAA CAGACCTCAG TGAGACAGAG GAAGCGGCGC 
CGGGGTGGTA AAACATCATG GTGCCATGGG GGCGGTAGAT GTGGTGGTCA GGAGAGACTG 
GATAAAGCCC TAGCCAGCTT CTTGAGCTGG CAGCAGTCTG CAGAGGAGCG TCTCCTCTCA 
CTGGAGGAGG CGCGGCTGGA GAGAGAGCTG CAGGCGGAGG AGCGCAGGGA GCAGCGGGAG 
GAGAGGAGGG CAGAACAAGA GCGTCAGCAT GAGCTCCGCC TGTTCAGCAT GCTCACGGGG 
GTGTTGCTCG CTGTCAGAGA GGGTACACAA ACCACAACAC AAAGCGACCC CGCCACCTCA 
CCCTTAGCTC GTTTTTCAGC TTCGCTGATG ACAACGCCTG CCCCCTCTGC TCCTTCATCT 
TCATCCCAGC CACCTCCAGA AGCTCCCACG ACAGAGTCTA TTTTTAATCA GGAGACAAAA 
AAGTCACAGC TCACATCTGT CAAGGCCTGC AAAATGTCTC AGAATGCTTT GGAAACACTG 
AGGGGTGAGG AAGGTCCCGG CAATAGCGTC TACCTGTCTG AGCGTGGTAA TAGCATCCGA 
CTCCATGAAG GTATTCTCCA GGAGGGCTAC ATCCAATATG GAATGGACCG GCACCATGAT 
ACAGACAACC CTGATGGTAT CATCAACATG GGGACCAGTG AGAACAAACT TTGTTATGAT 
CTTCTTCATA AACGGCTGAC CAAGCCTGAC ATGCTGCACA TCAACCCATC CTTGTTGCAG 
TATTCAGACT GGAGGGGGCA CGCTTTCCTC AGGGAGGCAG TTGCAAAATT CCTGACTCAC 
TACTGTTGTT CTCCAAACCC ATTAAAAGCT GACAACGTTG TGGTGATGAA TGGCTGTGGT 
TCCCTGTTCT CATGTATTGC TGCAGTCATT TGTGATCCTA AAGATGCCAT CCTTATTCCG 
ACTCCTTTCT ACGGCGTCAT CACTGAAGAT CTTCATCTGT ATAGTGATGT AAAACTCTTC 
CACGTTCCCC TTGATTGTGA GGCTGATGGC AAAGATAACC GACCCTTCCG CCTCACTGTA 
GGGAAACTAG AAGAAGGTCT GCAAAGAGCT AAGGAAGAGG GGTTGACAAT TCGCGGCGTT 
ATCCTGATGA ACCCCCACAA CCCTCTGGCT GAAATCTACA GCCTGAAGGA AATGATTACC 
TTCCTGGAGT TTGCCAAAAG AAATGCTCTC CATGCCATTG TAGATGAAGT GTACATGCTG 
ACAGTGTTTG ATGAATCTGT CACCTTTCAG AGTGTCCTCA GTGCAGACAG TTTGCCCGAC 
CCACAGAGGA CGCATGTAAT GTGGGGAATG AGCAAGGACT TTGCAATGGC AGGAATCAGA 
ATAGGCACTT TATATACTGA GAACAGAGAC CTCGTGGAGG CTTTGGCCAA ATTGGGCTCT 
TTTCATGGTG TCCCTGGGAC TACCCAGCAC CAGGTTGCAC AACTGCTTCA GGACAAAGAG 
TGGATCAATG AGGAATTTTT GCCTGAGAAC AGACGCAGGC TGAAAGCTGC GTGCAGCTAC 
ATGACTGGAG AGCTGCTCAG TATGGGCATC CCGTTTCTAG ACAGGCCTGC GACACTCTAT 
GTCTGGGCTG ACCTTAGAAA GTATCTCCAA GAGTCATCGT TTGAGGAGGA GCTGTCTCTG 
TGGAAGTGTT TCCTCAGGCA CAAGGTGCTG CTGAGTTGTG GCCAAGCGTT CTGCTGTTCA 
GTGCCCGGGT GGTTCCGCAT CGTCTTTGCA GACCAACAGC ACCACCTTCA GCTTGGCCTT 
AAGCGAATTA GAGAGGCTTT AAAAGAAACT GAAGAAAAAA TGAGAGTCCC GGACATCAAA 
CAAACCAGTG AGGAGAGCCA AAAGCCAGTA AAAAAGGACA ATGCAGGTTC AGACAATGCT 
GCTACTGTCA ATCCAAAAGC CTTAGCCGAG AACAATTCAT CAGATCAGCT GAAGGAGAGG 
GATAGCCCTG TTCCTGACAC AGGCTCACTG GCCACTGAAG ACTGCCAAGC CTCTAAGCCC 
GCAGAGAGTC TTGACACGCT GATCGGTACT CTCAGGCATC AGATCCGTTC CTCCGATTGG 
CTGGAGAAAA GCACTCCAGA GCTGTCTGCT GGGGAGGACC CGGAAATTTT TGATGTGTTC 
AAGGTCCTGC TGCAAAGAGC CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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LOC100708325 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム