pum2 gene cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

mRNA	Protein	Name
XM_013271988.2	XP_013127442.1	pumilio homolog 2 isoform X2
XM_013271989.2	XP_013127443.1	pumilio homolog 2 isoform X3
XM_003446310.4	XP_003446358.1	pumilio homolog 2 isoform X8
XM_013271989.3	XP_013127443.1	pumilio homolog 2 isoform X3
XM_013271990.3	XP_013127444.1	pumilio homolog 2 isoform X5
XM_005474961.4	XP_005475018.1	pumilio homolog 2 isoform X7
XM_005474962.4	XP_005475019.1	pumilio homolog 2 isoform X9
XM_013271991.2	XP_013127445.1	pumilio homolog 2 isoform X6
XM_005474961.3	XP_005475018.1	pumilio homolog 2 isoform X7
XM_013271986.3	XP_013127440.1	pumilio homolog 2 isoform X1
XM_013271991.3	XP_013127445.1	pumilio homolog 2 isoform X6
XM_003446309.4	XP_003446357.1	pumilio homolog 2 isoform X4
XM_005474962.3	XP_005475019.1	pumilio homolog 2 isoform X9
XM_003446310.5	XP_003446358.1	pumilio homolog 2 isoform X8
XM_013271986.2	XP_013127440.1	pumilio homolog 2 isoform X1
XM_013271990.2	XP_013127444.1	pumilio homolog 2 isoform X5
XM_013271988.3	XP_013127442.1	pumilio homolog 2 isoform X2
XM_003446309.5	XP_003446357.1	pumilio homolog 2 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr40014	XM_013271986.3 Latest version!	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40015	XM_013271988.3 Latest version!	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40016	XM_013271989.3 Latest version!	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40017	XM_003446309.5 Latest version!	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40018	XM_013271990.3 Latest version!	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40019	XM_013271991.3 Latest version!	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40020	XM_005474961.4 Latest version!	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40021	XM_003446310.5 Latest version!	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40022	XM_005474962.4 Latest version!	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OOr40016	XM_013271989.2	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40020	XM_005474961.3	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40019	XM_013271991.2	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40022	XM_005474962.3	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40021	XM_003446310.4	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40014	XM_013271986.2	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40018	XM_013271990.2	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40015	XM_013271988.2	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr40017	XM_003446309.4	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

pum2 ( XM_013271986.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127440.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr40014
Clone ID Related Accession (Same CDS sequence)	XM_013271986.3 , XM_013271986.2
Accession Version	XM_013271986.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_013271986.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGGTAAT TAGCGACATT GTCCGTGAGC TTGATGGCCA TGTGCTGAAG 
TGTGTGAAAG ACCAGAATGG GAACCATGTG GTACAGAAGT GCATTGAATG TGTCCAGCCT 
CAGGCCCTTC AGTTCATCAT TGATGCCTTC CAGGGACAGG TTTTTGTACT TTCCACACAC 
CCCTATGGCT GCAGAGTTAT CCAAAGGATC TTGGAGCACT GCACCCAGGA GCAAACTCTG 
CCCATCCTGG AAGAGCTTCA TCAGCACTCT GAACAGCTGG GCCAGAAATA TCAAGGCGTA 
TCATTGGAGA TGACACCCAA AACATATTAT ACAGTGTCCC GTGATGCACT GTTCAAGGAT 
CAGTACGGTA ACTACGTCAT TCAGCATGTT TTGGAGCATG GCAGACCAGA AGATAAGAGC 
AAGATAGTGG CAGAGGTGCG CGGGAAGGTT CTTGTCCTGA GCCAGCATAA ATTTGCAAGT 
AATGTGGTGG AGAAGTGTGT GATCCACTCC TCGCGTGCAG AGAGAGCTCT GCTGATAGAT 
GAAGTGTGCT GCCAGAAGGA CGGGCCCCAC AGCGCCTTGT ACACCATGAT GAAAGACCAG 
TACGCCAACT ATGTTGTCCA AAGAATGATC GACATGGCAG AACCTGCTCA GCGTAAAATC 
ATCATGCACA AGATCCGGCC TCACATTGCC ACTTTGCGTA AGTACACCTA CGGGAAGCAC 
ATTCTAGCCA AGCTAGAGAA GTACTACATG AAGAGTGGAT CTGAACTGGG TCCAATCGGC 
GGTCCCACGA ATGGTCTGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127440.1
CDS	486..4169
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271986.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271986.3

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGGTAAT TAGCGACATT GTCCGTGAGC TTGATGGCCA TGTGCTGAAG 
TGTGTGAAAG ACCAGAATGG GAACCATGTG GTACAGAAGT GCATTGAATG TGTCCAGCCT 
CAGGCCCTTC AGTTCATCAT TGATGCCTTC CAGGGACAGG TTTTTGTACT TTCCACACAC 
CCCTATGGCT GCAGAGTTAT CCAAAGGATC TTGGAGCACT GCACCCAGGA GCAAACTCTG 
CCCATCCTGG AAGAGCTTCA TCAGCACTCT GAACAGCTGG GCCAGAAATA TCAAGGCGTA 
TCATTGGAGA TGACACCCAA AACATATTAT ACAGTGTCCC GTGATGCACT GTTCAAGGAT 
CAGTACGGTA ACTACGTCAT TCAGCATGTT TTGGAGCATG GCAGACCAGA AGATAAGAGC 
AAGATAGTGG CAGAGGTGCG CGGGAAGGTT CTTGTCCTGA GCCAGCATAA ATTTGCAAGT 
AATGTGGTGG AGAAGTGTGT GATCCACTCC TCGCGTGCAG AGAGAGCTCT GCTGATAGAT 
GAAGTGTGCT GCCAGAAGGA CGGGCCCCAC AGCGCCTTGT ACACCATGAT GAAAGACCAG 
TACGCCAACT ATGTTGTCCA AAGAATGATC GACATGGCAG AACCTGCTCA GCGTAAAATC 
ATCATGCACA AGATCCGGCC TCACATTGCC ACTTTGCGTA AGTACACCTA CGGGAAGCAC 
ATTCTAGCCA AGCTAGAGAA GTACTACATG AAGAGTGGAT CTGAACTGGG TCCAATCGGC 
GGTCCCACGA ATGGTCTGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_013271986.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127440.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr40014
Clone ID Related Accession (Same CDS sequence)	XM_013271986.3 , XM_013271986.2
Accession Version	XM_013271986.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_013271986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGGTAAT TAGCGACATT GTCCGTGAGC TTGATGGCCA TGTGCTGAAG 
TGTGTGAAAG ACCAGAATGG GAACCATGTG GTACAGAAGT GCATTGAATG TGTCCAGCCT 
CAGGCCCTTC AGTTCATCAT TGATGCCTTC CAGGGACAGG TTTTTGTACT TTCCACACAC 
CCCTATGGCT GCAGAGTTAT CCAAAGGATC TTGGAGCACT GCACCCAGGA GCAAACTCTG 
CCCATCCTGG AAGAGCTTCA TCAGCACTCT GAACAGCTGG GCCAGAAATA TCAAGGCGTA 
TCATTGGAGA TGACACCCAA AACATATTAT ACAGTGTCCC GTGATGCACT GTTCAAGGAT 
CAGTACGGTA ACTACGTCAT TCAGCATGTT TTGGAGCATG GCAGACCAGA AGATAAGAGC 
AAGATAGTGG CAGAGGTGCG CGGGAAGGTT CTTGTCCTGA GCCAGCATAA ATTTGCAAGT 
AATGTGGTGG AGAAGTGTGT GATCCACTCC TCGCGTGCAG AGAGAGCTCT GCTGATAGAT 
GAAGTGTGCT GCCAGAAGGA CGGGCCCCAC AGCGCCTTGT ACACCATGAT GAAAGACCAG 
TACGCCAACT ATGTTGTCCA AAGAATGATC GACATGGCAG AACCTGCTCA GCGTAAAATC 
ATCATGCACA AGATCCGGCC TCACATTGCC ACTTTGCGTA AGTACACCTA CGGGAAGCAC 
ATTCTAGCCA AGCTAGAGAA GTACTACATG AAGAGTGGAT CTGAACTGGG TCCAATCGGC 
GGTCCCACGA ATGGTCTGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127440.1
CDS	446..4129
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271986.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271986.2

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGGTAAT TAGCGACATT GTCCGTGAGC TTGATGGCCA TGTGCTGAAG 
TGTGTGAAAG ACCAGAATGG GAACCATGTG GTACAGAAGT GCATTGAATG TGTCCAGCCT 
CAGGCCCTTC AGTTCATCAT TGATGCCTTC CAGGGACAGG TTTTTGTACT TTCCACACAC 
CCCTATGGCT GCAGAGTTAT CCAAAGGATC TTGGAGCACT GCACCCAGGA GCAAACTCTG 
CCCATCCTGG AAGAGCTTCA TCAGCACTCT GAACAGCTGG GCCAGAAATA TCAAGGCGTA 
TCATTGGAGA TGACACCCAA AACATATTAT ACAGTGTCCC GTGATGCACT GTTCAAGGAT 
CAGTACGGTA ACTACGTCAT TCAGCATGTT TTGGAGCATG GCAGACCAGA AGATAAGAGC 
AAGATAGTGG CAGAGGTGCG CGGGAAGGTT CTTGTCCTGA GCCAGCATAA ATTTGCAAGT 
AATGTGGTGG AGAAGTGTGT GATCCACTCC TCGCGTGCAG AGAGAGCTCT GCTGATAGAT 
GAAGTGTGCT GCCAGAAGGA CGGGCCCCAC AGCGCCTTGT ACACCATGAT GAAAGACCAG 
TACGCCAACT ATGTTGTCCA AAGAATGATC GACATGGCAG AACCTGCTCA GCGTAAAATC 
ATCATGCACA AGATCCGGCC TCACATTGCC ACTTTGCGTA AGTACACCTA CGGGAAGCAC 
ATTCTAGCCA AGCTAGAGAA GTACTACATG AAGAGTGGAT CTGAACTGGG TCCAATCGGC 
GGTCCCACGA ATGGTCTGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_013271988.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127442.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X2, mRNA.

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CloneID	OOr40015
Clone ID Related Accession (Same CDS sequence)	XM_013271988.2 , XM_013271988.3
Accession Version	XM_013271988.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_013271988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG TGTCTGCATC TGGCTCCGGT 
AACAACATGT CTGGTCCTCC AGCCAACGGA CTGTACCGCT CCATGCCTCA GGCTCAACCT 
CAGCCGCAGC AGCAGCAGGC TCCTCCACCC AGCAGCGGCC TGCCCTCCAG CTCATTCTAC 
GGATCTGGAT CAGTCCCTAA CACCTCTCAG AGCAGCTCCC TTTTCTCTCA CACCTCTGCT 
GCCCCACCAC CCCCAAGCTC TTCCCTGGGC TTCAGCAGCA CCGGCGGCTC TCTTGGCGTT 
GGCCTGGGCT CTGCTCTTGG GGGTTTTGGC TCTTCTGTAT CCAGCTCGAC CAGTAGCAGT 
GTATCCCGCA GGGATTCCCT GTTGGCAAGT TCTGACCTAT ACAAACGAGG TGGCAGCAGT 
TTAACTCCCA TTGGGCAGCC GTTTTATAAC AGCCTGGGTT ACTCCTCTTC ACCTAGCCCC 
ATTGGCCTTA CACCAGGTCA TTCCCCACTC ACTCCTCCGC CATCTTTGCC CTCTTCCCAT 
GGATCCTCTT CCAGCCTTCA CCTAGGTGGC CTGACAAATG GCAGTGGTCG TTACATTTCT 
GCAGCTCCCG GAGCTGAGGC CAAATACCGG AGCACAGGCA GCACGTCCAG TCTGTTCAAT 
TCCAGCAGCC AGTTGTTCCC TCCCTCACGG CCCCGCTACA GCCGCTCTGA TGTCATGCCA 
TCTGGACGCA GCCGCCTATT GGAAGACTTC AGGAACAACC GTTTTCCAAA CCTCCAGCTC 
CGTGACTTGC CTGGACACAT GGTGGAGTTC TCTCAAGACC AGCACGGATC CAGATTTATC 
CAGCAGAAGC TAGAGAGGGC CACTCCTGCT GAGAGACAGA TGGTGTTTGG AGAGATTTTG 
CAAGCAGCAT ACCAACTGAT GACTGATGTA TTTGGGAATT ATGTCATCCA AAAGTTCTTT 
GAGTTTGGAA GTGCAGACCA GAAACTTGCT TTAGCGACCC GTATCCGCGG ACACGTCCTT 
CCCCTGGCGT TGCAGATGTA TGGGTGCAGG GTCATTCAGA AAGCCCTGGA GTCCATTTCC 
TCAGACCAGC AGGTAATTAG CGACATTGTC CGTGAGCTTG ATGGCCATGT GCTGAAGTGT 
GTGAAAGACC AGAATGGGAA CCATGTGGTA CAGAAGTGCA TTGAATGTGT CCAGCCTCAG 
GCCCTTCAGT TCATCATTGA TGCCTTCCAG GGACAGGTTT TTGTACTTTC CACACACCCC 
TATGGCTGCA GAGTTATCCA AAGGATCTTG GAGCACTGCA CCCAGGAGCA AACTCTGCCC 
ATCCTGGAAG AGCTTCATCA GCACTCTGAA CAGCTGGGCC AGAAATATCA AGGCGTATCA 
TTGGAGATGA CACCCAAAAC ATATTATACA GTGTCCCGTG ATGCACTGTT CAAGGATCAG 
TACGGTAACT ACGTCATTCA GCATGTTTTG GAGCATGGCA GACCAGAAGA TAAGAGCAAG 
ATAGTGGCAG AGGTGCGCGG GAAGGTTCTT GTCCTGAGCC AGCATAAATT TGCAAGTAAT 
GTGGTGGAGA AGTGTGTGAT CCACTCCTCG CGTGCAGAGA GAGCTCTGCT GATAGATGAA 
GTGTGCTGCC AGAAGGACGG GCCCCACAGC GCCTTGTACA CCATGATGAA AGACCAGTAC 
GCCAACTATG TTGTCCAAAG AATGATCGAC ATGGCAGAAC CTGCTCAGCG TAAAATCATC 
ATGCACAAGA TCCGGCCTCA CATTGCCACT TTGCGTAAGT ACACCTACGG GAAGCACATT 
CTAGCCAAGC TAGAGAAGTA CTACATGAAG AGTGGATCTG AACTGGGTCC AATCGGCGGT 
CCCACGAATG GTCTGATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127442.1
CDS	446..4126
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271988.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271988.2

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG TGTCTGCATC TGGCTCCGGT 
AACAACATGT CTGGTCCTCC AGCCAACGGA CTGTACCGCT CCATGCCTCA GGCTCAACCT 
CAGCCGCAGC AGCAGCAGGC TCCTCCACCC AGCAGCGGCC TGCCCTCCAG CTCATTCTAC 
GGATCTGGAT CAGTCCCTAA CACCTCTCAG AGCAGCTCCC TTTTCTCTCA CACCTCTGCT 
GCCCCACCAC CCCCAAGCTC TTCCCTGGGC TTCAGCAGCA CCGGCGGCTC TCTTGGCGTT 
GGCCTGGGCT CTGCTCTTGG GGGTTTTGGC TCTTCTGTAT CCAGCTCGAC CAGTAGCAGT 
GTATCCCGCA GGGATTCCCT GTTGGCAAGT TCTGACCTAT ACAAACGAGG TGGCAGCAGT 
TTAACTCCCA TTGGGCAGCC GTTTTATAAC AGCCTGGGTT ACTCCTCTTC ACCTAGCCCC 
ATTGGCCTTA CACCAGGTCA TTCCCCACTC ACTCCTCCGC CATCTTTGCC CTCTTCCCAT 
GGATCCTCTT CCAGCCTTCA CCTAGGTGGC CTGACAAATG GCAGTGGTCG TTACATTTCT 
GCAGCTCCCG GAGCTGAGGC CAAATACCGG AGCACAGGCA GCACGTCCAG TCTGTTCAAT 
TCCAGCAGCC AGTTGTTCCC TCCCTCACGG CCCCGCTACA GCCGCTCTGA TGTCATGCCA 
TCTGGACGCA GCCGCCTATT GGAAGACTTC AGGAACAACC GTTTTCCAAA CCTCCAGCTC 
CGTGACTTGC CTGGACACAT GGTGGAGTTC TCTCAAGACC AGCACGGATC CAGATTTATC 
CAGCAGAAGC TAGAGAGGGC CACTCCTGCT GAGAGACAGA TGGTGTTTGG AGAGATTTTG 
CAAGCAGCAT ACCAACTGAT GACTGATGTA TTTGGGAATT ATGTCATCCA AAAGTTCTTT 
GAGTTTGGAA GTGCAGACCA GAAACTTGCT TTAGCGACCC GTATCCGCGG ACACGTCCTT 
CCCCTGGCGT TGCAGATGTA TGGGTGCAGG GTCATTCAGA AAGCCCTGGA GTCCATTTCC 
TCAGACCAGC AGGTAATTAG CGACATTGTC CGTGAGCTTG ATGGCCATGT GCTGAAGTGT 
GTGAAAGACC AGAATGGGAA CCATGTGGTA CAGAAGTGCA TTGAATGTGT CCAGCCTCAG 
GCCCTTCAGT TCATCATTGA TGCCTTCCAG GGACAGGTTT TTGTACTTTC CACACACCCC 
TATGGCTGCA GAGTTATCCA AAGGATCTTG GAGCACTGCA CCCAGGAGCA AACTCTGCCC 
ATCCTGGAAG AGCTTCATCA GCACTCTGAA CAGCTGGGCC AGAAATATCA AGGCGTATCA 
TTGGAGATGA CACCCAAAAC ATATTATACA GTGTCCCGTG ATGCACTGTT CAAGGATCAG 
TACGGTAACT ACGTCATTCA GCATGTTTTG GAGCATGGCA GACCAGAAGA TAAGAGCAAG 
ATAGTGGCAG AGGTGCGCGG GAAGGTTCTT GTCCTGAGCC AGCATAAATT TGCAAGTAAT 
GTGGTGGAGA AGTGTGTGAT CCACTCCTCG CGTGCAGAGA GAGCTCTGCT GATAGATGAA 
GTGTGCTGCC AGAAGGACGG GCCCCACAGC GCCTTGTACA CCATGATGAA AGACCAGTAC 
GCCAACTATG TTGTCCAAAG AATGATCGAC ATGGCAGAAC CTGCTCAGCG TAAAATCATC 
ATGCACAAGA TCCGGCCTCA CATTGCCACT TTGCGTAAGT ACACCTACGG GAAGCACATT 
CTAGCCAAGC TAGAGAAGTA CTACATGAAG AGTGGATCTG AACTGGGTCC AATCGGCGGT 
CCCACGAATG GTCTGATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_013271988.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127442.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOr40015
Clone ID Related Accession (Same CDS sequence)	XM_013271988.2 , XM_013271988.3
Accession Version	XM_013271988.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_013271988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG TGTCTGCATC TGGCTCCGGT 
AACAACATGT CTGGTCCTCC AGCCAACGGA CTGTACCGCT CCATGCCTCA GGCTCAACCT 
CAGCCGCAGC AGCAGCAGGC TCCTCCACCC AGCAGCGGCC TGCCCTCCAG CTCATTCTAC 
GGATCTGGAT CAGTCCCTAA CACCTCTCAG AGCAGCTCCC TTTTCTCTCA CACCTCTGCT 
GCCCCACCAC CCCCAAGCTC TTCCCTGGGC TTCAGCAGCA CCGGCGGCTC TCTTGGCGTT 
GGCCTGGGCT CTGCTCTTGG GGGTTTTGGC TCTTCTGTAT CCAGCTCGAC CAGTAGCAGT 
GTATCCCGCA GGGATTCCCT GTTGGCAAGT TCTGACCTAT ACAAACGAGG TGGCAGCAGT 
TTAACTCCCA TTGGGCAGCC GTTTTATAAC AGCCTGGGTT ACTCCTCTTC ACCTAGCCCC 
ATTGGCCTTA CACCAGGTCA TTCCCCACTC ACTCCTCCGC CATCTTTGCC CTCTTCCCAT 
GGATCCTCTT CCAGCCTTCA CCTAGGTGGC CTGACAAATG GCAGTGGTCG TTACATTTCT 
GCAGCTCCCG GAGCTGAGGC CAAATACCGG AGCACAGGCA GCACGTCCAG TCTGTTCAAT 
TCCAGCAGCC AGTTGTTCCC TCCCTCACGG CCCCGCTACA GCCGCTCTGA TGTCATGCCA 
TCTGGACGCA GCCGCCTATT GGAAGACTTC AGGAACAACC GTTTTCCAAA CCTCCAGCTC 
CGTGACTTGC CTGGACACAT GGTGGAGTTC TCTCAAGACC AGCACGGATC CAGATTTATC 
CAGCAGAAGC TAGAGAGGGC CACTCCTGCT GAGAGACAGA TGGTGTTTGG AGAGATTTTG 
CAAGCAGCAT ACCAACTGAT GACTGATGTA TTTGGGAATT ATGTCATCCA AAAGTTCTTT 
GAGTTTGGAA GTGCAGACCA GAAACTTGCT TTAGCGACCC GTATCCGCGG ACACGTCCTT 
CCCCTGGCGT TGCAGATGTA TGGGTGCAGG GTCATTCAGA AAGCCCTGGA GTCCATTTCC 
TCAGACCAGC AGGTAATTAG CGACATTGTC CGTGAGCTTG ATGGCCATGT GCTGAAGTGT 
GTGAAAGACC AGAATGGGAA CCATGTGGTA CAGAAGTGCA TTGAATGTGT CCAGCCTCAG 
GCCCTTCAGT TCATCATTGA TGCCTTCCAG GGACAGGTTT TTGTACTTTC CACACACCCC 
TATGGCTGCA GAGTTATCCA AAGGATCTTG GAGCACTGCA CCCAGGAGCA AACTCTGCCC 
ATCCTGGAAG AGCTTCATCA GCACTCTGAA CAGCTGGGCC AGAAATATCA AGGCGTATCA 
TTGGAGATGA CACCCAAAAC ATATTATACA GTGTCCCGTG ATGCACTGTT CAAGGATCAG 
TACGGTAACT ACGTCATTCA GCATGTTTTG GAGCATGGCA GACCAGAAGA TAAGAGCAAG 
ATAGTGGCAG AGGTGCGCGG GAAGGTTCTT GTCCTGAGCC AGCATAAATT TGCAAGTAAT 
GTGGTGGAGA AGTGTGTGAT CCACTCCTCG CGTGCAGAGA GAGCTCTGCT GATAGATGAA 
GTGTGCTGCC AGAAGGACGG GCCCCACAGC GCCTTGTACA CCATGATGAA AGACCAGTAC 
GCCAACTATG TTGTCCAAAG AATGATCGAC ATGGCAGAAC CTGCTCAGCG TAAAATCATC 
ATGCACAAGA TCCGGCCTCA CATTGCCACT TTGCGTAAGT ACACCTACGG GAAGCACATT 
CTAGCCAAGC TAGAGAAGTA CTACATGAAG AGTGGATCTG AACTGGGTCC AATCGGCGGT 
CCCACGAATG GTCTGATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127442.1
CDS	486..4166
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271988.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271988.3

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG TGTCTGCATC TGGCTCCGGT 
AACAACATGT CTGGTCCTCC AGCCAACGGA CTGTACCGCT CCATGCCTCA GGCTCAACCT 
CAGCCGCAGC AGCAGCAGGC TCCTCCACCC AGCAGCGGCC TGCCCTCCAG CTCATTCTAC 
GGATCTGGAT CAGTCCCTAA CACCTCTCAG AGCAGCTCCC TTTTCTCTCA CACCTCTGCT 
GCCCCACCAC CCCCAAGCTC TTCCCTGGGC TTCAGCAGCA CCGGCGGCTC TCTTGGCGTT 
GGCCTGGGCT CTGCTCTTGG GGGTTTTGGC TCTTCTGTAT CCAGCTCGAC CAGTAGCAGT 
GTATCCCGCA GGGATTCCCT GTTGGCAAGT TCTGACCTAT ACAAACGAGG TGGCAGCAGT 
TTAACTCCCA TTGGGCAGCC GTTTTATAAC AGCCTGGGTT ACTCCTCTTC ACCTAGCCCC 
ATTGGCCTTA CACCAGGTCA TTCCCCACTC ACTCCTCCGC CATCTTTGCC CTCTTCCCAT 
GGATCCTCTT CCAGCCTTCA CCTAGGTGGC CTGACAAATG GCAGTGGTCG TTACATTTCT 
GCAGCTCCCG GAGCTGAGGC CAAATACCGG AGCACAGGCA GCACGTCCAG TCTGTTCAAT 
TCCAGCAGCC AGTTGTTCCC TCCCTCACGG CCCCGCTACA GCCGCTCTGA TGTCATGCCA 
TCTGGACGCA GCCGCCTATT GGAAGACTTC AGGAACAACC GTTTTCCAAA CCTCCAGCTC 
CGTGACTTGC CTGGACACAT GGTGGAGTTC TCTCAAGACC AGCACGGATC CAGATTTATC 
CAGCAGAAGC TAGAGAGGGC CACTCCTGCT GAGAGACAGA TGGTGTTTGG AGAGATTTTG 
CAAGCAGCAT ACCAACTGAT GACTGATGTA TTTGGGAATT ATGTCATCCA AAAGTTCTTT 
GAGTTTGGAA GTGCAGACCA GAAACTTGCT TTAGCGACCC GTATCCGCGG ACACGTCCTT 
CCCCTGGCGT TGCAGATGTA TGGGTGCAGG GTCATTCAGA AAGCCCTGGA GTCCATTTCC 
TCAGACCAGC AGGTAATTAG CGACATTGTC CGTGAGCTTG ATGGCCATGT GCTGAAGTGT 
GTGAAAGACC AGAATGGGAA CCATGTGGTA CAGAAGTGCA TTGAATGTGT CCAGCCTCAG 
GCCCTTCAGT TCATCATTGA TGCCTTCCAG GGACAGGTTT TTGTACTTTC CACACACCCC 
TATGGCTGCA GAGTTATCCA AAGGATCTTG GAGCACTGCA CCCAGGAGCA AACTCTGCCC 
ATCCTGGAAG AGCTTCATCA GCACTCTGAA CAGCTGGGCC AGAAATATCA AGGCGTATCA 
TTGGAGATGA CACCCAAAAC ATATTATACA GTGTCCCGTG ATGCACTGTT CAAGGATCAG 
TACGGTAACT ACGTCATTCA GCATGTTTTG GAGCATGGCA GACCAGAAGA TAAGAGCAAG 
ATAGTGGCAG AGGTGCGCGG GAAGGTTCTT GTCCTGAGCC AGCATAAATT TGCAAGTAAT 
GTGGTGGAGA AGTGTGTGAT CCACTCCTCG CGTGCAGAGA GAGCTCTGCT GATAGATGAA 
GTGTGCTGCC AGAAGGACGG GCCCCACAGC GCCTTGTACA CCATGATGAA AGACCAGTAC 
GCCAACTATG TTGTCCAAAG AATGATCGAC ATGGCAGAAC CTGCTCAGCG TAAAATCATC 
ATGCACAAGA TCCGGCCTCA CATTGCCACT TTGCGTAAGT ACACCTACGG GAAGCACATT 
CTAGCCAAGC TAGAGAAGTA CTACATGAAG AGTGGATCTG AACTGGGTCC AATCGGCGGT 
CCCACGAATG GTCTGATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_013271989.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127443.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOr40016
Clone ID Related Accession (Same CDS sequence)	XM_013271989.2 , XM_013271989.3
Accession Version	XM_013271989.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_013271989.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGAGCGA CATTGTCCGT GAGCTTGATG GCCATGTGCT GAAGTGTGTG 
AAAGACCAGA ATGGGAACCA TGTGGTACAG AAGTGCATTG AATGTGTCCA GCCTCAGGCC 
CTTCAGTTCA TCATTGATGC CTTCCAGGGA CAGGTTTTTG TACTTTCCAC ACACCCCTAT 
GGCTGCAGAG TTATCCAAAG GATCTTGGAG CACTGCACCC AGGAGCAAAC TCTGCCCATC 
CTGGAAGAGC TTCATCAGCA CTCTGAACAG CTGGGCCAGA AATATCAAGG CGTATCATTG 
GAGATGACAC CCAAAACATA TTATACAGTG TCCCGTGATG CACTGTTCAA GGATCAGTAC 
GGTAACTACG TCATTCAGCA TGTTTTGGAG CATGGCAGAC CAGAAGATAA GAGCAAGATA 
GTGGCAGAGG TGCGCGGGAA GGTTCTTGTC CTGAGCCAGC ATAAATTTGC AAGTAATGTG 
GTGGAGAAGT GTGTGATCCA CTCCTCGCGT GCAGAGAGAG CTCTGCTGAT AGATGAAGTG 
TGCTGCCAGA AGGACGGGCC CCACAGCGCC TTGTACACCA TGATGAAAGA CCAGTACGCC 
AACTATGTTG TCCAAAGAAT GATCGACATG GCAGAACCTG CTCAGCGTAA AATCATCATG 
CACAAGATCC GGCCTCACAT TGCCACTTTG CGTAAGTACA CCTACGGGAA GCACATTCTA 
GCCAAGCTAG AGAAGTACTA CATGAAGAGT GGATCTGAAC TGGGTCCAAT CGGCGGTCCC 
ACGAATGGTC TGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127443.1
CDS	446..4123
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271989.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271989.2

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGAGCGA CATTGTCCGT GAGCTTGATG GCCATGTGCT GAAGTGTGTG 
AAAGACCAGA ATGGGAACCA TGTGGTACAG AAGTGCATTG AATGTGTCCA GCCTCAGGCC 
CTTCAGTTCA TCATTGATGC CTTCCAGGGA CAGGTTTTTG TACTTTCCAC ACACCCCTAT 
GGCTGCAGAG TTATCCAAAG GATCTTGGAG CACTGCACCC AGGAGCAAAC TCTGCCCATC 
CTGGAAGAGC TTCATCAGCA CTCTGAACAG CTGGGCCAGA AATATCAAGG CGTATCATTG 
GAGATGACAC CCAAAACATA TTATACAGTG TCCCGTGATG CACTGTTCAA GGATCAGTAC 
GGTAACTACG TCATTCAGCA TGTTTTGGAG CATGGCAGAC CAGAAGATAA GAGCAAGATA 
GTGGCAGAGG TGCGCGGGAA GGTTCTTGTC CTGAGCCAGC ATAAATTTGC AAGTAATGTG 
GTGGAGAAGT GTGTGATCCA CTCCTCGCGT GCAGAGAGAG CTCTGCTGAT AGATGAAGTG 
TGCTGCCAGA AGGACGGGCC CCACAGCGCC TTGTACACCA TGATGAAAGA CCAGTACGCC 
AACTATGTTG TCCAAAGAAT GATCGACATG GCAGAACCTG CTCAGCGTAA AATCATCATG 
CACAAGATCC GGCCTCACAT TGCCACTTTG CGTAAGTACA CCTACGGGAA GCACATTCTA 
GCCAAGCTAG AGAAGTACTA CATGAAGAGT GGATCTGAAC TGGGTCCAAT CGGCGGTCCC 
ACGAATGGTC TGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_013271989.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127443.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X3, mRNA.

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CloneID	OOr40016
Clone ID Related Accession (Same CDS sequence)	XM_013271989.2 , XM_013271989.3
Accession Version	XM_013271989.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_013271989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGAGCGA CATTGTCCGT GAGCTTGATG GCCATGTGCT GAAGTGTGTG 
AAAGACCAGA ATGGGAACCA TGTGGTACAG AAGTGCATTG AATGTGTCCA GCCTCAGGCC 
CTTCAGTTCA TCATTGATGC CTTCCAGGGA CAGGTTTTTG TACTTTCCAC ACACCCCTAT 
GGCTGCAGAG TTATCCAAAG GATCTTGGAG CACTGCACCC AGGAGCAAAC TCTGCCCATC 
CTGGAAGAGC TTCATCAGCA CTCTGAACAG CTGGGCCAGA AATATCAAGG CGTATCATTG 
GAGATGACAC CCAAAACATA TTATACAGTG TCCCGTGATG CACTGTTCAA GGATCAGTAC 
GGTAACTACG TCATTCAGCA TGTTTTGGAG CATGGCAGAC CAGAAGATAA GAGCAAGATA 
GTGGCAGAGG TGCGCGGGAA GGTTCTTGTC CTGAGCCAGC ATAAATTTGC AAGTAATGTG 
GTGGAGAAGT GTGTGATCCA CTCCTCGCGT GCAGAGAGAG CTCTGCTGAT AGATGAAGTG 
TGCTGCCAGA AGGACGGGCC CCACAGCGCC TTGTACACCA TGATGAAAGA CCAGTACGCC 
AACTATGTTG TCCAAAGAAT GATCGACATG GCAGAACCTG CTCAGCGTAA AATCATCATG 
CACAAGATCC GGCCTCACAT TGCCACTTTG CGTAAGTACA CCTACGGGAA GCACATTCTA 
GCCAAGCTAG AGAAGTACTA CATGAAGAGT GGATCTGAAC TGGGTCCAAT CGGCGGTCCC 
ACGAATGGTC TGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127443.1
CDS	486..4163
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271989.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271989.3

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGAGCGA CATTGTCCGT GAGCTTGATG GCCATGTGCT GAAGTGTGTG 
AAAGACCAGA ATGGGAACCA TGTGGTACAG AAGTGCATTG AATGTGTCCA GCCTCAGGCC 
CTTCAGTTCA TCATTGATGC CTTCCAGGGA CAGGTTTTTG TACTTTCCAC ACACCCCTAT 
GGCTGCAGAG TTATCCAAAG GATCTTGGAG CACTGCACCC AGGAGCAAAC TCTGCCCATC 
CTGGAAGAGC TTCATCAGCA CTCTGAACAG CTGGGCCAGA AATATCAAGG CGTATCATTG 
GAGATGACAC CCAAAACATA TTATACAGTG TCCCGTGATG CACTGTTCAA GGATCAGTAC 
GGTAACTACG TCATTCAGCA TGTTTTGGAG CATGGCAGAC CAGAAGATAA GAGCAAGATA 
GTGGCAGAGG TGCGCGGGAA GGTTCTTGTC CTGAGCCAGC ATAAATTTGC AAGTAATGTG 
GTGGAGAAGT GTGTGATCCA CTCCTCGCGT GCAGAGAGAG CTCTGCTGAT AGATGAAGTG 
TGCTGCCAGA AGGACGGGCC CCACAGCGCC TTGTACACCA TGATGAAAGA CCAGTACGCC 
AACTATGTTG TCCAAAGAAT GATCGACATG GCAGAACCTG CTCAGCGTAA AATCATCATG 
CACAAGATCC GGCCTCACAT TGCCACTTTG CGTAAGTACA CCTACGGGAA GCACATTCTA 
GCCAAGCTAG AGAAGTACTA CATGAAGAGT GGATCTGAAC TGGGTCCAAT CGGCGGTCCC 
ACGAATGGTC TGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_003446309.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003446357.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOr40017
Clone ID Related Accession (Same CDS sequence)	XM_003446309.4 , XM_003446309.5
Accession Version	XM_003446309.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_003446309.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGGTAA TTAGCGACAT TGTCCGTGAG 
CTTGATGGCC ATGTGCTGAA GTGTGTGAAA GACCAGAATG GGAACCATGT GGTACAGAAG 
TGCATTGAAT GTGTCCAGCC TCAGGCCCTT CAGTTCATCA TTGATGCCTT CCAGGGACAG 
GTTTTTGTAC TTTCCACACA CCCCTATGGC TGCAGAGTTA TCCAAAGGAT CTTGGAGCAC 
TGCACCCAGG AGCAAACTCT GCCCATCCTG GAAGAGCTTC ATCAGCACTC TGAACAGCTG 
GGCCAGAAAT ATCAAGGCGT ATCATTGGAG ATGACACCCA AAACATATTA TACAGTGTCC 
CGTGATGCAC TGTTCAAGGA TCAGTACGGT AACTACGTCA TTCAGCATGT TTTGGAGCAT 
GGCAGACCAG AAGATAAGAG CAAGATAGTG GCAGAGGTGC GCGGGAAGGT TCTTGTCCTG 
AGCCAGCATA AATTTGCAAG TAATGTGGTG GAGAAGTGTG TGATCCACTC CTCGCGTGCA 
GAGAGAGCTC TGCTGATAGA TGAAGTGTGC TGCCAGAAGG ACGGGCCCCA CAGCGCCTTG 
TACACCATGA TGAAAGACCA GTACGCCAAC TATGTTGTCC AAAGAATGAT CGACATGGCA 
GAACCTGCTC AGCGTAAAAT CATCATGCAC AAGATCCGGC CTCACATTGC CACTTTGCGT 
AAGTACACCT ACGGGAAGCA CATTCTAGCC AAGCTAGAGA AGTACTACAT GAAGAGTGGA 
TCTGAACTGG GTCCAATCGG CGGTCCCACG AATGGTCTGA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003446357.1
CDS	446..4090
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_003446309.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003446309.4

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGGTAA TTAGCGACAT TGTCCGTGAG 
CTTGATGGCC ATGTGCTGAA GTGTGTGAAA GACCAGAATG GGAACCATGT GGTACAGAAG 
TGCATTGAAT GTGTCCAGCC TCAGGCCCTT CAGTTCATCA TTGATGCCTT CCAGGGACAG 
GTTTTTGTAC TTTCCACACA CCCCTATGGC TGCAGAGTTA TCCAAAGGAT CTTGGAGCAC 
TGCACCCAGG AGCAAACTCT GCCCATCCTG GAAGAGCTTC ATCAGCACTC TGAACAGCTG 
GGCCAGAAAT ATCAAGGCGT ATCATTGGAG ATGACACCCA AAACATATTA TACAGTGTCC 
CGTGATGCAC TGTTCAAGGA TCAGTACGGT AACTACGTCA TTCAGCATGT TTTGGAGCAT 
GGCAGACCAG AAGATAAGAG CAAGATAGTG GCAGAGGTGC GCGGGAAGGT TCTTGTCCTG 
AGCCAGCATA AATTTGCAAG TAATGTGGTG GAGAAGTGTG TGATCCACTC CTCGCGTGCA 
GAGAGAGCTC TGCTGATAGA TGAAGTGTGC TGCCAGAAGG ACGGGCCCCA CAGCGCCTTG 
TACACCATGA TGAAAGACCA GTACGCCAAC TATGTTGTCC AAAGAATGAT CGACATGGCA 
GAACCTGCTC AGCGTAAAAT CATCATGCAC AAGATCCGGC CTCACATTGC CACTTTGCGT 
AAGTACACCT ACGGGAAGCA CATTCTAGCC AAGCTAGAGA AGTACTACAT GAAGAGTGGA 
TCTGAACTGG GTCCAATCGG CGGTCCCACG AATGGTCTGA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_003446309.5 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003446357.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOr40017
Clone ID Related Accession (Same CDS sequence)	XM_003446309.4 , XM_003446309.5
Accession Version	XM_003446309.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_003446309.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGGTAA TTAGCGACAT TGTCCGTGAG 
CTTGATGGCC ATGTGCTGAA GTGTGTGAAA GACCAGAATG GGAACCATGT GGTACAGAAG 
TGCATTGAAT GTGTCCAGCC TCAGGCCCTT CAGTTCATCA TTGATGCCTT CCAGGGACAG 
GTTTTTGTAC TTTCCACACA CCCCTATGGC TGCAGAGTTA TCCAAAGGAT CTTGGAGCAC 
TGCACCCAGG AGCAAACTCT GCCCATCCTG GAAGAGCTTC ATCAGCACTC TGAACAGCTG 
GGCCAGAAAT ATCAAGGCGT ATCATTGGAG ATGACACCCA AAACATATTA TACAGTGTCC 
CGTGATGCAC TGTTCAAGGA TCAGTACGGT AACTACGTCA TTCAGCATGT TTTGGAGCAT 
GGCAGACCAG AAGATAAGAG CAAGATAGTG GCAGAGGTGC GCGGGAAGGT TCTTGTCCTG 
AGCCAGCATA AATTTGCAAG TAATGTGGTG GAGAAGTGTG TGATCCACTC CTCGCGTGCA 
GAGAGAGCTC TGCTGATAGA TGAAGTGTGC TGCCAGAAGG ACGGGCCCCA CAGCGCCTTG 
TACACCATGA TGAAAGACCA GTACGCCAAC TATGTTGTCC AAAGAATGAT CGACATGGCA 
GAACCTGCTC AGCGTAAAAT CATCATGCAC AAGATCCGGC CTCACATTGC CACTTTGCGT 
AAGTACACCT ACGGGAAGCA CATTCTAGCC AAGCTAGAGA AGTACTACAT GAAGAGTGGA 
TCTGAACTGG GTCCAATCGG CGGTCCCACG AATGGTCTGA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003446357.1
CDS	486..4130
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_003446309.5
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003446309.5

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGGTAA TTAGCGACAT TGTCCGTGAG 
CTTGATGGCC ATGTGCTGAA GTGTGTGAAA GACCAGAATG GGAACCATGT GGTACAGAAG 
TGCATTGAAT GTGTCCAGCC TCAGGCCCTT CAGTTCATCA TTGATGCCTT CCAGGGACAG 
GTTTTTGTAC TTTCCACACA CCCCTATGGC TGCAGAGTTA TCCAAAGGAT CTTGGAGCAC 
TGCACCCAGG AGCAAACTCT GCCCATCCTG GAAGAGCTTC ATCAGCACTC TGAACAGCTG 
GGCCAGAAAT ATCAAGGCGT ATCATTGGAG ATGACACCCA AAACATATTA TACAGTGTCC 
CGTGATGCAC TGTTCAAGGA TCAGTACGGT AACTACGTCA TTCAGCATGT TTTGGAGCAT 
GGCAGACCAG AAGATAAGAG CAAGATAGTG GCAGAGGTGC GCGGGAAGGT TCTTGTCCTG 
AGCCAGCATA AATTTGCAAG TAATGTGGTG GAGAAGTGTG TGATCCACTC CTCGCGTGCA 
GAGAGAGCTC TGCTGATAGA TGAAGTGTGC TGCCAGAAGG ACGGGCCCCA CAGCGCCTTG 
TACACCATGA TGAAAGACCA GTACGCCAAC TATGTTGTCC AAAGAATGAT CGACATGGCA 
GAACCTGCTC AGCGTAAAAT CATCATGCAC AAGATCCGGC CTCACATTGC CACTTTGCGT 
AAGTACACCT ACGGGAAGCA CATTCTAGCC AAGCTAGAGA AGTACTACAT GAAGAGTGGA 
TCTGAACTGG GTCCAATCGG CGGTCCCACG AATGGTCTGA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_013271990.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127444.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X5, mRNA.

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CloneID	OOr40018
Clone ID Related Accession (Same CDS sequence)	XM_013271990.3 , XM_013271990.2
Accession Version	XM_013271990.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_013271990.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGGTAAT TAGCGACATT GTCCGTGAGC TTGATGGCCA TGTGCTGAAG 
TGTGTGAAAG ACCAGAATGG GAACCATGTG GTACAGAAGT GCATTGAATG TGTCCAGCCT 
CAGGCCCTTC AGTTCATCAT TGATGCCTTC CAGGGACAGG TTTTTGTACT TTCCACACAC 
CCCTATGGCT GCAGAGTTAT CCAAAGGATC TTGGAGCACT GCACCCAGGA GCAAACTCTG 
CCCATCCTGG AAGAGCTTCA TCAGCACTCT GAACAGCTGG GCCAGGATCA GTACGGTAAC 
TACGTCATTC AGCATGTTTT GGAGCATGGC AGACCAGAAG ATAAGAGCAA GATAGTGGCA 
GAGGTGCGCG GGAAGGTTCT TGTCCTGAGC CAGCATAAAT TTGCAAGTAA TGTGGTGGAG 
AAGTGTGTGA TCCACTCCTC GCGTGCAGAG AGAGCTCTGC TGATAGATGA AGTGTGCTGC 
CAGAAGGACG GGCCCCACAG CGCCTTGTAC ACCATGATGA AAGACCAGTA CGCCAACTAT 
GTTGTCCAAA GAATGATCGA CATGGCAGAA CCTGCTCAGC GTAAAATCAT CATGCACAAG 
ATCCGGCCTC ACATTGCCAC TTTGCGTAAG TACACCTACG GGAAGCACAT TCTAGCCAAG 
CTAGAGAAGT ACTACATGAA GAGTGGATCT GAACTGGGTC CAATCGGCGG TCCCACGAAT 
GGTCTGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127444.1
CDS	486..4097
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271990.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271990.3

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGGTAAT TAGCGACATT GTCCGTGAGC TTGATGGCCA TGTGCTGAAG 
TGTGTGAAAG ACCAGAATGG GAACCATGTG GTACAGAAGT GCATTGAATG TGTCCAGCCT 
CAGGCCCTTC AGTTCATCAT TGATGCCTTC CAGGGACAGG TTTTTGTACT TTCCACACAC 
CCCTATGGCT GCAGAGTTAT CCAAAGGATC TTGGAGCACT GCACCCAGGA GCAAACTCTG 
CCCATCCTGG AAGAGCTTCA TCAGCACTCT GAACAGCTGG GCCAGGATCA GTACGGTAAC 
TACGTCATTC AGCATGTTTT GGAGCATGGC AGACCAGAAG ATAAGAGCAA GATAGTGGCA 
GAGGTGCGCG GGAAGGTTCT TGTCCTGAGC CAGCATAAAT TTGCAAGTAA TGTGGTGGAG 
AAGTGTGTGA TCCACTCCTC GCGTGCAGAG AGAGCTCTGC TGATAGATGA AGTGTGCTGC 
CAGAAGGACG GGCCCCACAG CGCCTTGTAC ACCATGATGA AAGACCAGTA CGCCAACTAT 
GTTGTCCAAA GAATGATCGA CATGGCAGAA CCTGCTCAGC GTAAAATCAT CATGCACAAG 
ATCCGGCCTC ACATTGCCAC TTTGCGTAAG TACACCTACG GGAAGCACAT TCTAGCCAAG 
CTAGAGAAGT ACTACATGAA GAGTGGATCT GAACTGGGTC CAATCGGCGG TCCCACGAAT 
GGTCTGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_013271990.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127444.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOr40018
Clone ID Related Accession (Same CDS sequence)	XM_013271990.3 , XM_013271990.2
Accession Version	XM_013271990.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_013271990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGGTAAT TAGCGACATT GTCCGTGAGC TTGATGGCCA TGTGCTGAAG 
TGTGTGAAAG ACCAGAATGG GAACCATGTG GTACAGAAGT GCATTGAATG TGTCCAGCCT 
CAGGCCCTTC AGTTCATCAT TGATGCCTTC CAGGGACAGG TTTTTGTACT TTCCACACAC 
CCCTATGGCT GCAGAGTTAT CCAAAGGATC TTGGAGCACT GCACCCAGGA GCAAACTCTG 
CCCATCCTGG AAGAGCTTCA TCAGCACTCT GAACAGCTGG GCCAGGATCA GTACGGTAAC 
TACGTCATTC AGCATGTTTT GGAGCATGGC AGACCAGAAG ATAAGAGCAA GATAGTGGCA 
GAGGTGCGCG GGAAGGTTCT TGTCCTGAGC CAGCATAAAT TTGCAAGTAA TGTGGTGGAG 
AAGTGTGTGA TCCACTCCTC GCGTGCAGAG AGAGCTCTGC TGATAGATGA AGTGTGCTGC 
CAGAAGGACG GGCCCCACAG CGCCTTGTAC ACCATGATGA AAGACCAGTA CGCCAACTAT 
GTTGTCCAAA GAATGATCGA CATGGCAGAA CCTGCTCAGC GTAAAATCAT CATGCACAAG 
ATCCGGCCTC ACATTGCCAC TTTGCGTAAG TACACCTACG GGAAGCACAT TCTAGCCAAG 
CTAGAGAAGT ACTACATGAA GAGTGGATCT GAACTGGGTC CAATCGGCGG TCCCACGAAT 
GGTCTGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127444.1
CDS	446..4057
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271990.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271990.2

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGGTAAT TAGCGACATT GTCCGTGAGC TTGATGGCCA TGTGCTGAAG 
TGTGTGAAAG ACCAGAATGG GAACCATGTG GTACAGAAGT GCATTGAATG TGTCCAGCCT 
CAGGCCCTTC AGTTCATCAT TGATGCCTTC CAGGGACAGG TTTTTGTACT TTCCACACAC 
CCCTATGGCT GCAGAGTTAT CCAAAGGATC TTGGAGCACT GCACCCAGGA GCAAACTCTG 
CCCATCCTGG AAGAGCTTCA TCAGCACTCT GAACAGCTGG GCCAGGATCA GTACGGTAAC 
TACGTCATTC AGCATGTTTT GGAGCATGGC AGACCAGAAG ATAAGAGCAA GATAGTGGCA 
GAGGTGCGCG GGAAGGTTCT TGTCCTGAGC CAGCATAAAT TTGCAAGTAA TGTGGTGGAG 
AAGTGTGTGA TCCACTCCTC GCGTGCAGAG AGAGCTCTGC TGATAGATGA AGTGTGCTGC 
CAGAAGGACG GGCCCCACAG CGCCTTGTAC ACCATGATGA AAGACCAGTA CGCCAACTAT 
GTTGTCCAAA GAATGATCGA CATGGCAGAA CCTGCTCAGC GTAAAATCAT CATGCACAAG 
ATCCGGCCTC ACATTGCCAC TTTGCGTAAG TACACCTACG GGAAGCACAT TCTAGCCAAG 
CTAGAGAAGT ACTACATGAA GAGTGGATCT GAACTGGGTC CAATCGGCGG TCCCACGAAT 
GGTCTGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_013271991.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127445.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X6, mRNA.

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CloneID	OOr40019
Clone ID Related Accession (Same CDS sequence)	XM_013271991.2 , XM_013271991.3
Accession Version	XM_013271991.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_013271991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGAGCGA CATTGTCCGT GAGCTTGATG GCCATGTGCT GAAGTGTGTG 
AAAGACCAGA ATGGGAACCA TGTGGTACAG AAGTGCATTG AATGTGTCCA GCCTCAGGCC 
CTTCAGTTCA TCATTGATGC CTTCCAGGGA CAGGTTTTTG TACTTTCCAC ACACCCCTAT 
GGCTGCAGAG TTATCCAAAG GATCTTGGAG CACTGCACCC AGGAGCAAAC TCTGCCCATC 
CTGGAAGAGC TTCATCAGCA CTCTGAACAG CTGGGCCAGG ATCAGTACGG TAACTACGTC 
ATTCAGCATG TTTTGGAGCA TGGCAGACCA GAAGATAAGA GCAAGATAGT GGCAGAGGTG 
CGCGGGAAGG TTCTTGTCCT GAGCCAGCAT AAATTTGCAA GTAATGTGGT GGAGAAGTGT 
GTGATCCACT CCTCGCGTGC AGAGAGAGCT CTGCTGATAG ATGAAGTGTG CTGCCAGAAG 
GACGGGCCCC ACAGCGCCTT GTACACCATG ATGAAAGACC AGTACGCCAA CTATGTTGTC 
CAAAGAATGA TCGACATGGC AGAACCTGCT CAGCGTAAAA TCATCATGCA CAAGATCCGG 
CCTCACATTG CCACTTTGCG TAAGTACACC TACGGGAAGC ACATTCTAGC CAAGCTAGAG 
AAGTACTACA TGAAGAGTGG ATCTGAACTG GGTCCAATCG GCGGTCCCAC GAATGGTCTG 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127445.1
CDS	446..4051
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271991.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271991.2

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGAGCGA CATTGTCCGT GAGCTTGATG GCCATGTGCT GAAGTGTGTG 
AAAGACCAGA ATGGGAACCA TGTGGTACAG AAGTGCATTG AATGTGTCCA GCCTCAGGCC 
CTTCAGTTCA TCATTGATGC CTTCCAGGGA CAGGTTTTTG TACTTTCCAC ACACCCCTAT 
GGCTGCAGAG TTATCCAAAG GATCTTGGAG CACTGCACCC AGGAGCAAAC TCTGCCCATC 
CTGGAAGAGC TTCATCAGCA CTCTGAACAG CTGGGCCAGG ATCAGTACGG TAACTACGTC 
ATTCAGCATG TTTTGGAGCA TGGCAGACCA GAAGATAAGA GCAAGATAGT GGCAGAGGTG 
CGCGGGAAGG TTCTTGTCCT GAGCCAGCAT AAATTTGCAA GTAATGTGGT GGAGAAGTGT 
GTGATCCACT CCTCGCGTGC AGAGAGAGCT CTGCTGATAG ATGAAGTGTG CTGCCAGAAG 
GACGGGCCCC ACAGCGCCTT GTACACCATG ATGAAAGACC AGTACGCCAA CTATGTTGTC 
CAAAGAATGA TCGACATGGC AGAACCTGCT CAGCGTAAAA TCATCATGCA CAAGATCCGG 
CCTCACATTG CCACTTTGCG TAAGTACACC TACGGGAAGC ACATTCTAGC CAAGCTAGAG 
AAGTACTACA TGAAGAGTGG ATCTGAACTG GGTCCAATCG GCGGTCCCAC GAATGGTCTG 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_013271991.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_013127445.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X6, mRNA.

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CloneID	OOr40019
Clone ID Related Accession (Same CDS sequence)	XM_013271991.2 , XM_013271991.3
Accession Version	XM_013271991.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_013271991.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGAGCGA CATTGTCCGT GAGCTTGATG GCCATGTGCT GAAGTGTGTG 
AAAGACCAGA ATGGGAACCA TGTGGTACAG AAGTGCATTG AATGTGTCCA GCCTCAGGCC 
CTTCAGTTCA TCATTGATGC CTTCCAGGGA CAGGTTTTTG TACTTTCCAC ACACCCCTAT 
GGCTGCAGAG TTATCCAAAG GATCTTGGAG CACTGCACCC AGGAGCAAAC TCTGCCCATC 
CTGGAAGAGC TTCATCAGCA CTCTGAACAG CTGGGCCAGG ATCAGTACGG TAACTACGTC 
ATTCAGCATG TTTTGGAGCA TGGCAGACCA GAAGATAAGA GCAAGATAGT GGCAGAGGTG 
CGCGGGAAGG TTCTTGTCCT GAGCCAGCAT AAATTTGCAA GTAATGTGGT GGAGAAGTGT 
GTGATCCACT CCTCGCGTGC AGAGAGAGCT CTGCTGATAG ATGAAGTGTG CTGCCAGAAG 
GACGGGCCCC ACAGCGCCTT GTACACCATG ATGAAAGACC AGTACGCCAA CTATGTTGTC 
CAAAGAATGA TCGACATGGC AGAACCTGCT CAGCGTAAAA TCATCATGCA CAAGATCCGG 
CCTCACATTG CCACTTTGCG TAAGTACACC TACGGGAAGC ACATTCTAGC CAAGCTAGAG 
AAGTACTACA TGAAGAGTGG ATCTGAACTG GGTCCAATCG GCGGTCCCAC GAATGGTCTG 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013127445.1
CDS	486..4091
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_013271991.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013271991.3

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCATCAT TCAGTGTCTC AGCCAATCAT GGTGCAGCGA 
CGGCCAGGCC AGGGTTTCCA TGGGAATGGT GATGCCAATT CTGTGCTTTC ACCTCGCTCA 
GAAGGCGGAG GTCTCGGCGT GAGCATGGTG GAGTACGTCC TGAGTTCGTC CCCTGGTGAC 
AAGATGGATG GCCGCTACAG GAATGGTGGC TATGGTGCAG GAGATGCTGA CCAAGATGGG 
AGAGAGAAAA GTGATGCGCA AGAGAAGGTG TCGCCTTTTG AAGAGGACAA AAGCCCTGAG 
ATGAAGGTGG GAGAGGAGAG TGATCCTACT AAAGCCAATG GAAGAGGTCT GCTGAACGGC 
ATGGACAGAG ACTGTAAAGA CTTCAACCCA ACTCCTGGAA GCCGTCAAGC TTCTCCCACT 
GAGGCTGTGG AGAGGATGGG TCCCAGTCAG ACAGGTTTGG AGATGATGGG GCAGCACCAC 
CACCCTCATG TTCTCCAACA GCAGAACCCC TCCCAAAACA AGGTCCAAGC TGAGGATTTC 
CAGAACCAGG AGGCCCAGAA CATGGGCGGT ATGGAGCAGC AAGCTGGTGT GGAGTCCTTA 
CAGTTTGACT ATGCTGGTAA TCAGATTCAG GTGGACTCTT CAGGGACTCC AGTTGGATTG 
TTTGACTACA ACTCTCAGCA GCAGTTGTTC CAGAGGTCTA ATCCATTGAC TGTTCAACAG 
CTCACTGCAG CTCAGCAGCA ACAATATGCA CTGGCTGCAG CCCAGCAGCA GCATCTTGCT 
GGCCTTGCTC CTGCGTTTGT GCCAAACCCT TACATCATTA ATGCTGCCCC ACCTGGAGCT 
GATCCCTACA CTGCTGCTGG GTTGGCAGCA GCAGCAACGC TTGCAGGCCC CACAGTTGTT 
CCACCACAGT ACTACGGCGT CCCTTGGGGT GTGTACCCAG CTAATCTTTT CCAGCAGCAG 
CCTGCATCTA CTGCCAATCA CTCGGCCAAT CAGCAAGCAT CCAGTCAAGG GCCAGGGCCA 
GGGCAACCAC AGGTGATGCG CACTGGAACC AACCAGCGAC CTCTTACACC TGGGCAAGGC 
CAGCAGAGCC AGCAGGAATC CCTAGCTGCA GCAGCTGCTG CAAACCCTGC ATTGGCATAC 
ACAGGAATGC CTGGTTATCA GGTTTTGGCC CCTGCAGCTT ACTATGACCA GACTGGAGCT 
TTGGTAATGG GCCCTGGTGC CCGCACCGGT CTGGGTGGGC CTGTTCGTCT AGTCCAAACC 
CCACTTCTTA TCAACCATGC AGCGGCGCAG GCTGCAGCAG CAGTGTCTGC ATCTGGCTCC 
GGTAACAACA TGTCTGGTCC TCCAGCCAAC GGACTGTACC GCTCCATGCC TCAGGCTCAA 
CCTCAGCCGC AGCAGCAGCA GGCTCCTCCA CCCAGCAGCG GCCTGCCCTC CAGCTCATTC 
TACGGATCTG GATCAGTCCC TAACACCTCT CAGAGCAGCT CCCTTTTCTC TCACACCTCT 
GCTGCCCCAC CACCCCCAAG CTCTTCCCTG GGCTTCAGCA GCACCGGCGG CTCTCTTGGC 
GTTGGCCTGG GCTCTGCTCT TGGGGGTTTT GGCTCTTCTG TATCCAGCTC GACCAGTAGC 
AGTGTATCCC GCAGGGATTC CCTGTTGGCA AGTTCTGACC TATACAAACG AGGTGGCAGC 
AGTTTAACTC CCATTGGGCA GCCGTTTTAT AACAGCCTGG GTTACTCCTC TTCACCTAGC 
CCCATTGGCC TTACACCAGG TCATTCCCCA CTCACTCCTC CGCCATCTTT GCCCTCTTCC 
CATGGATCCT CTTCCAGCCT TCACCTAGGT GGCCTGACAA ATGGCAGTGG TCGTTACATT 
TCTGCAGCTC CCGGAGCTGA GGCCAAATAC CGGAGCACAG GCAGCACGTC CAGTCTGTTC 
AATTCCAGCA GCCAGTTGTT CCCTCCCTCA CGGCCCCGCT ACAGCCGCTC TGATGTCATG 
CCATCTGGAC GCAGCCGCCT ATTGGAAGAC TTCAGGAACA ACCGTTTTCC AAACCTCCAG 
CTCCGTGACT TGCCTGGACA CATGGTGGAG TTCTCTCAAG ACCAGCACGG ATCCAGATTT 
ATCCAGCAGA AGCTAGAGAG GGCCACTCCT GCTGAGAGAC AGATGGTGTT TGGAGAGATT 
TTGCAAGCAG CATACCAACT GATGACTGAT GTATTTGGGA ATTATGTCAT CCAAAAGTTC 
TTTGAGTTTG GAAGTGCAGA CCAGAAACTT GCTTTAGCGA CCCGTATCCG CGGACACGTC 
CTTCCCCTGG CGTTGCAGAT GTATGGGTGC AGGGTCATTC AGAAAGCCCT GGAGTCCATT 
TCCTCAGACC AGCAGAGCGA CATTGTCCGT GAGCTTGATG GCCATGTGCT GAAGTGTGTG 
AAAGACCAGA ATGGGAACCA TGTGGTACAG AAGTGCATTG AATGTGTCCA GCCTCAGGCC 
CTTCAGTTCA TCATTGATGC CTTCCAGGGA CAGGTTTTTG TACTTTCCAC ACACCCCTAT 
GGCTGCAGAG TTATCCAAAG GATCTTGGAG CACTGCACCC AGGAGCAAAC TCTGCCCATC 
CTGGAAGAGC TTCATCAGCA CTCTGAACAG CTGGGCCAGG ATCAGTACGG TAACTACGTC 
ATTCAGCATG TTTTGGAGCA TGGCAGACCA GAAGATAAGA GCAAGATAGT GGCAGAGGTG 
CGCGGGAAGG TTCTTGTCCT GAGCCAGCAT AAATTTGCAA GTAATGTGGT GGAGAAGTGT 
GTGATCCACT CCTCGCGTGC AGAGAGAGCT CTGCTGATAG ATGAAGTGTG CTGCCAGAAG 
GACGGGCCCC ACAGCGCCTT GTACACCATG ATGAAAGACC AGTACGCCAA CTATGTTGTC 
CAAAGAATGA TCGACATGGC AGAACCTGCT CAGCGTAAAA TCATCATGCA CAAGATCCGG 
CCTCACATTG CCACTTTGCG TAAGTACACC TACGGGAAGC ACATTCTAGC CAAGCTAGAG 
AAGTACTACA TGAAGAGTGG ATCTGAACTG GGTCCAATCG GCGGTCCCAC GAATGGTCTG 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_005474961.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005475018.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OOr40020
Clone ID Related Accession (Same CDS sequence)	XM_005474961.4 , XM_005474961.3
Accession Version	XM_005474961.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_005474961.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCAAGGC GGAGGTCTCG GCGTGAGCAT GGTGGAGTAC 
GTCCTGAGTT CGTCCCCTGG TGACAAGATG GATGGCCGCT ACAGGAATGG TGGCTATGGT 
GCAGGAGATG CTGACCAAGA TGGGAGAGAG AAAAGTGATG CGCAAGAGAA GGTGTCGCCT 
TTTGAAGAGG ACAAAAGCCC TGAGATGAAG GTGGGAGAGG AGAGTGATCC TACTAAAGCC 
AATGGAAGAG GTCTGCTGAA CGGCATGGAC AGAGACTGTA AAGACTTCAA CCCAACTCCT 
GGAAGCCGTC AAGCTTCTCC CACTGAGGCT GTGGAGAGGA TGGGTCCCAG TCAGACAGGT 
TTGGAGATGA TGGGGCAGCA CCACCACCCT CATGTTCTCC AACAGCAGAA CCCCTCCCAA 
AACAAGGTCC AAGCTGAGGA TTTCCAGAAC CAGGAGGCCC AGAACATGGG CGGTATGGAG 
CAGCAAGCTG GTGTGGAGTC CTTACAGTTT GACTATGCTG GTAATCAGAT TCAGGTGGAC 
TCTTCAGGGA CTCCAGTTGG ATTGTTTGAC TACAACTCTC AGCAGCAGTT GTTCCAGAGG 
TCTAATCCAT TGACTGTTCA ACAGCTCACT GCAGCTCAGC AGCAACAATA TGCACTGGCT 
GCAGCCCAGC AGCAGCATCT TGCTGGCCTT GCTCCTGCGT TTGTGCCAAA CCCTTACATC 
ATTAATGCTG CCCCACCTGG AGCTGATCCC TACACTGCTG CTGGGTTGGC AGCAGCAGCA 
ACGCTTGCAG GCCCCACAGT TGTTCCACCA CAGTACTACG GCGTCCCTTG GGGTGTGTAC 
CCAGCTAATC TTTTCCAGCA GCAGCCTGCA TCTACTGCCA ATCACTCGGC CAATCAGCAA 
GCATCCAGTC AAGGGCCAGG GCCAGGGCAA CCACAGGTGA TGCGCACTGG AACCAACCAG 
CGACCTCTTA CACCTGGGCA AGGCCAGCAG AGCCAGCAGG AATCCCTAGC TGCAGCAGCT 
GCTGCAAACC CTGCATTGGC ATACACAGGA ATGCCTGGTT ATCAGGTTTT GGCCCCTGCA 
GCTTACTATG ACCAGACTGG AGCTTTGGTA ATGGGCCCTG GTGCCCGCAC CGGTCTGGGT 
GGGCCTGTTC GTCTAGTCCA AACCCCACTT CTTATCAACC ATGCAGCGGC GCAGGCTGCA 
GCAGCAGTGT CTGCATCTGG CTCCGGTAAC AACATGTCTG GTCCTCCAGC CAACGGACTG 
TACCGCTCCA TGCCTCAGGC TCAACCTCAG CCGCAGCAGC AGCAGGCTCC TCCACCCAGC 
AGCGGCCTGC CCTCCAGCTC ATTCTACGGA TCTGGATCAG TCCCTAACAC CTCTCAGAGC 
AGCTCCCTTT TCTCTCACAC CTCTGCTGCC CCACCACCCC CAAGCTCTTC CCTGGGCTTC 
AGCAGCACCG GCGGCTCTCT TGGCGTTGGC CTGGGCTCTG CTCTTGGGGG TTTTGGCTCT 
TCTGTATCCA GCTCGACCAG TAGCAGTGTA TCCCGCAGGG ATTCCCTGTT GGCAAGTTCT 
GACCTATACA AACGAGGTGG CAGCAGTTTA ACTCCCATTG GGCAGCCGTT TTATAACAGC 
CTGGGTTACT CCTCTTCACC TAGCCCCATT GGCCTTACAC CAGGTCATTC CCCACTCACT 
CCTCCGCCAT CTTTGCCCTC TTCCCATGGA TCCTCTTCCA GCCTTCACCT AGGTGGCCTG 
ACAAATGGCA GTGGTCGTTA CATTTCTGCA GCTCCCGGAG CTGAGGCCAA ATACCGGAGC 
ACAGGCAGCA CGTCCAGTCT GTTCAATTCC AGCAGCCAGT TGTTCCCTCC CTCACGGCCC 
CGCTACAGCC GCTCTGATGT CATGCCATCT GGACGCAGCC GCCTATTGGA AGACTTCAGG 
AACAACCGTT TTCCAAACCT CCAGCTCCGT GACTTGCCTG GACACATGGT GGAGTTCTCT 
CAAGACCAGC ACGGATCCAG ATTTATCCAG CAGAAGCTAG AGAGGGCCAC TCCTGCTGAG 
AGACAGATGG TGTTTGGAGA GATTTTGCAA GCAGCATACC AACTGATGAC TGATGTATTT 
GGGAATTATG TCATCCAAAA GTTCTTTGAG TTTGGAAGTG CAGACCAGAA ACTTGCTTTA 
GCGACCCGTA TCCGCGGACA CGTCCTTCCC CTGGCGTTGC AGATGTATGG GTGCAGGGTC 
ATTCAGAAAG CCCTGGAGTC CATTTCCTCA GACCAGCAGG TAATTAGCGA CATTGTCCGT 
GAGCTTGATG GCCATGTGCT GAAGTGTGTG AAAGACCAGA ATGGGAACCA TGTGGTACAG 
AAGTGCATTG AATGTGTCCA GCCTCAGGCC CTTCAGTTCA TCATTGATGC CTTCCAGGGA 
CAGGTTTTTG TACTTTCCAC ACACCCCTAT GGCTGCAGAG TTATCCAAAG GATCTTGGAG 
CACTGCACCC AGGAGCAAAC TCTGCCCATC CTGGAAGAGC TTCATCAGCA CTCTGAACAG 
CTGGGCCAGA AATATCAAGG CGTATCATTG GAGATGACAC CCAAAACATA TTATACAGTG 
TCCCGTGATG CACTGTTCAA GGATCAGTAC GGTAACTACG TCATTCAGCA TGTTTTGGAG 
CATGGCAGAC CAGAAGATAA GAGCAAGATA GTGGCAGAGG TGCGCGGGAA GGTTCTTGTC 
CTGAGCCAGC ATAAATTTGC AAGTAATGTG GTGGAGAAGT GTGTGATCCA CTCCTCGCGT 
GCAGAGAGAG CTCTGCTGAT AGATGAAGTG TGCTGCCAGA AGGACGGGCC CCACAGCGCC 
TTGTACACCA TGATGAAAGA CCAGTACGCC AACTATGTTG TCCAAAGAAT GATCGACATG 
GCAGAACCTG CTCAGCGTAA AATCATCATG CACAAGATCC GGCCTCACAT TGCCACTTTG 
CGTAAGTACA CCTACGGGAA GCACATTCTA GCCAAGCTAG AGAAGTACTA CATGAAGAGT 
GGATCTGAAC TGGGTCCAAT CGGCGGTCCC ACGAATGGTC TGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005475018.1
CDS	486..4073
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSQGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_005474961.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005474961.4

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCAAGGC GGAGGTCTCG GCGTGAGCAT GGTGGAGTAC 
GTCCTGAGTT CGTCCCCTGG TGACAAGATG GATGGCCGCT ACAGGAATGG TGGCTATGGT 
GCAGGAGATG CTGACCAAGA TGGGAGAGAG AAAAGTGATG CGCAAGAGAA GGTGTCGCCT 
TTTGAAGAGG ACAAAAGCCC TGAGATGAAG GTGGGAGAGG AGAGTGATCC TACTAAAGCC 
AATGGAAGAG GTCTGCTGAA CGGCATGGAC AGAGACTGTA AAGACTTCAA CCCAACTCCT 
GGAAGCCGTC AAGCTTCTCC CACTGAGGCT GTGGAGAGGA TGGGTCCCAG TCAGACAGGT 
TTGGAGATGA TGGGGCAGCA CCACCACCCT CATGTTCTCC AACAGCAGAA CCCCTCCCAA 
AACAAGGTCC AAGCTGAGGA TTTCCAGAAC CAGGAGGCCC AGAACATGGG CGGTATGGAG 
CAGCAAGCTG GTGTGGAGTC CTTACAGTTT GACTATGCTG GTAATCAGAT TCAGGTGGAC 
TCTTCAGGGA CTCCAGTTGG ATTGTTTGAC TACAACTCTC AGCAGCAGTT GTTCCAGAGG 
TCTAATCCAT TGACTGTTCA ACAGCTCACT GCAGCTCAGC AGCAACAATA TGCACTGGCT 
GCAGCCCAGC AGCAGCATCT TGCTGGCCTT GCTCCTGCGT TTGTGCCAAA CCCTTACATC 
ATTAATGCTG CCCCACCTGG AGCTGATCCC TACACTGCTG CTGGGTTGGC AGCAGCAGCA 
ACGCTTGCAG GCCCCACAGT TGTTCCACCA CAGTACTACG GCGTCCCTTG GGGTGTGTAC 
CCAGCTAATC TTTTCCAGCA GCAGCCTGCA TCTACTGCCA ATCACTCGGC CAATCAGCAA 
GCATCCAGTC AAGGGCCAGG GCCAGGGCAA CCACAGGTGA TGCGCACTGG AACCAACCAG 
CGACCTCTTA CACCTGGGCA AGGCCAGCAG AGCCAGCAGG AATCCCTAGC TGCAGCAGCT 
GCTGCAAACC CTGCATTGGC ATACACAGGA ATGCCTGGTT ATCAGGTTTT GGCCCCTGCA 
GCTTACTATG ACCAGACTGG AGCTTTGGTA ATGGGCCCTG GTGCCCGCAC CGGTCTGGGT 
GGGCCTGTTC GTCTAGTCCA AACCCCACTT CTTATCAACC ATGCAGCGGC GCAGGCTGCA 
GCAGCAGTGT CTGCATCTGG CTCCGGTAAC AACATGTCTG GTCCTCCAGC CAACGGACTG 
TACCGCTCCA TGCCTCAGGC TCAACCTCAG CCGCAGCAGC AGCAGGCTCC TCCACCCAGC 
AGCGGCCTGC CCTCCAGCTC ATTCTACGGA TCTGGATCAG TCCCTAACAC CTCTCAGAGC 
AGCTCCCTTT TCTCTCACAC CTCTGCTGCC CCACCACCCC CAAGCTCTTC CCTGGGCTTC 
AGCAGCACCG GCGGCTCTCT TGGCGTTGGC CTGGGCTCTG CTCTTGGGGG TTTTGGCTCT 
TCTGTATCCA GCTCGACCAG TAGCAGTGTA TCCCGCAGGG ATTCCCTGTT GGCAAGTTCT 
GACCTATACA AACGAGGTGG CAGCAGTTTA ACTCCCATTG GGCAGCCGTT TTATAACAGC 
CTGGGTTACT CCTCTTCACC TAGCCCCATT GGCCTTACAC CAGGTCATTC CCCACTCACT 
CCTCCGCCAT CTTTGCCCTC TTCCCATGGA TCCTCTTCCA GCCTTCACCT AGGTGGCCTG 
ACAAATGGCA GTGGTCGTTA CATTTCTGCA GCTCCCGGAG CTGAGGCCAA ATACCGGAGC 
ACAGGCAGCA CGTCCAGTCT GTTCAATTCC AGCAGCCAGT TGTTCCCTCC CTCACGGCCC 
CGCTACAGCC GCTCTGATGT CATGCCATCT GGACGCAGCC GCCTATTGGA AGACTTCAGG 
AACAACCGTT TTCCAAACCT CCAGCTCCGT GACTTGCCTG GACACATGGT GGAGTTCTCT 
CAAGACCAGC ACGGATCCAG ATTTATCCAG CAGAAGCTAG AGAGGGCCAC TCCTGCTGAG 
AGACAGATGG TGTTTGGAGA GATTTTGCAA GCAGCATACC AACTGATGAC TGATGTATTT 
GGGAATTATG TCATCCAAAA GTTCTTTGAG TTTGGAAGTG CAGACCAGAA ACTTGCTTTA 
GCGACCCGTA TCCGCGGACA CGTCCTTCCC CTGGCGTTGC AGATGTATGG GTGCAGGGTC 
ATTCAGAAAG CCCTGGAGTC CATTTCCTCA GACCAGCAGG TAATTAGCGA CATTGTCCGT 
GAGCTTGATG GCCATGTGCT GAAGTGTGTG AAAGACCAGA ATGGGAACCA TGTGGTACAG 
AAGTGCATTG AATGTGTCCA GCCTCAGGCC CTTCAGTTCA TCATTGATGC CTTCCAGGGA 
CAGGTTTTTG TACTTTCCAC ACACCCCTAT GGCTGCAGAG TTATCCAAAG GATCTTGGAG 
CACTGCACCC AGGAGCAAAC TCTGCCCATC CTGGAAGAGC TTCATCAGCA CTCTGAACAG 
CTGGGCCAGA AATATCAAGG CGTATCATTG GAGATGACAC CCAAAACATA TTATACAGTG 
TCCCGTGATG CACTGTTCAA GGATCAGTAC GGTAACTACG TCATTCAGCA TGTTTTGGAG 
CATGGCAGAC CAGAAGATAA GAGCAAGATA GTGGCAGAGG TGCGCGGGAA GGTTCTTGTC 
CTGAGCCAGC ATAAATTTGC AAGTAATGTG GTGGAGAAGT GTGTGATCCA CTCCTCGCGT 
GCAGAGAGAG CTCTGCTGAT AGATGAAGTG TGCTGCCAGA AGGACGGGCC CCACAGCGCC 
TTGTACACCA TGATGAAAGA CCAGTACGCC AACTATGTTG TCCAAAGAAT GATCGACATG 
GCAGAACCTG CTCAGCGTAA AATCATCATG CACAAGATCC GGCCTCACAT TGCCACTTTG 
CGTAAGTACA CCTACGGGAA GCACATTCTA GCCAAGCTAG AGAAGTACTA CATGAAGAGT 
GGATCTGAAC TGGGTCCAAT CGGCGGTCCC ACGAATGGTC TGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_005474961.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005475018.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X7, mRNA.

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CloneID	OOr40020
Clone ID Related Accession (Same CDS sequence)	XM_005474961.4 , XM_005474961.3
Accession Version	XM_005474961.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_005474961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCAAGGC GGAGGTCTCG GCGTGAGCAT GGTGGAGTAC 
GTCCTGAGTT CGTCCCCTGG TGACAAGATG GATGGCCGCT ACAGGAATGG TGGCTATGGT 
GCAGGAGATG CTGACCAAGA TGGGAGAGAG AAAAGTGATG CGCAAGAGAA GGTGTCGCCT 
TTTGAAGAGG ACAAAAGCCC TGAGATGAAG GTGGGAGAGG AGAGTGATCC TACTAAAGCC 
AATGGAAGAG GTCTGCTGAA CGGCATGGAC AGAGACTGTA AAGACTTCAA CCCAACTCCT 
GGAAGCCGTC AAGCTTCTCC CACTGAGGCT GTGGAGAGGA TGGGTCCCAG TCAGACAGGT 
TTGGAGATGA TGGGGCAGCA CCACCACCCT CATGTTCTCC AACAGCAGAA CCCCTCCCAA 
AACAAGGTCC AAGCTGAGGA TTTCCAGAAC CAGGAGGCCC AGAACATGGG CGGTATGGAG 
CAGCAAGCTG GTGTGGAGTC CTTACAGTTT GACTATGCTG GTAATCAGAT TCAGGTGGAC 
TCTTCAGGGA CTCCAGTTGG ATTGTTTGAC TACAACTCTC AGCAGCAGTT GTTCCAGAGG 
TCTAATCCAT TGACTGTTCA ACAGCTCACT GCAGCTCAGC AGCAACAATA TGCACTGGCT 
GCAGCCCAGC AGCAGCATCT TGCTGGCCTT GCTCCTGCGT TTGTGCCAAA CCCTTACATC 
ATTAATGCTG CCCCACCTGG AGCTGATCCC TACACTGCTG CTGGGTTGGC AGCAGCAGCA 
ACGCTTGCAG GCCCCACAGT TGTTCCACCA CAGTACTACG GCGTCCCTTG GGGTGTGTAC 
CCAGCTAATC TTTTCCAGCA GCAGCCTGCA TCTACTGCCA ATCACTCGGC CAATCAGCAA 
GCATCCAGTC AAGGGCCAGG GCCAGGGCAA CCACAGGTGA TGCGCACTGG AACCAACCAG 
CGACCTCTTA CACCTGGGCA AGGCCAGCAG AGCCAGCAGG AATCCCTAGC TGCAGCAGCT 
GCTGCAAACC CTGCATTGGC ATACACAGGA ATGCCTGGTT ATCAGGTTTT GGCCCCTGCA 
GCTTACTATG ACCAGACTGG AGCTTTGGTA ATGGGCCCTG GTGCCCGCAC CGGTCTGGGT 
GGGCCTGTTC GTCTAGTCCA AACCCCACTT CTTATCAACC ATGCAGCGGC GCAGGCTGCA 
GCAGCAGTGT CTGCATCTGG CTCCGGTAAC AACATGTCTG GTCCTCCAGC CAACGGACTG 
TACCGCTCCA TGCCTCAGGC TCAACCTCAG CCGCAGCAGC AGCAGGCTCC TCCACCCAGC 
AGCGGCCTGC CCTCCAGCTC ATTCTACGGA TCTGGATCAG TCCCTAACAC CTCTCAGAGC 
AGCTCCCTTT TCTCTCACAC CTCTGCTGCC CCACCACCCC CAAGCTCTTC CCTGGGCTTC 
AGCAGCACCG GCGGCTCTCT TGGCGTTGGC CTGGGCTCTG CTCTTGGGGG TTTTGGCTCT 
TCTGTATCCA GCTCGACCAG TAGCAGTGTA TCCCGCAGGG ATTCCCTGTT GGCAAGTTCT 
GACCTATACA AACGAGGTGG CAGCAGTTTA ACTCCCATTG GGCAGCCGTT TTATAACAGC 
CTGGGTTACT CCTCTTCACC TAGCCCCATT GGCCTTACAC CAGGTCATTC CCCACTCACT 
CCTCCGCCAT CTTTGCCCTC TTCCCATGGA TCCTCTTCCA GCCTTCACCT AGGTGGCCTG 
ACAAATGGCA GTGGTCGTTA CATTTCTGCA GCTCCCGGAG CTGAGGCCAA ATACCGGAGC 
ACAGGCAGCA CGTCCAGTCT GTTCAATTCC AGCAGCCAGT TGTTCCCTCC CTCACGGCCC 
CGCTACAGCC GCTCTGATGT CATGCCATCT GGACGCAGCC GCCTATTGGA AGACTTCAGG 
AACAACCGTT TTCCAAACCT CCAGCTCCGT GACTTGCCTG GACACATGGT GGAGTTCTCT 
CAAGACCAGC ACGGATCCAG ATTTATCCAG CAGAAGCTAG AGAGGGCCAC TCCTGCTGAG 
AGACAGATGG TGTTTGGAGA GATTTTGCAA GCAGCATACC AACTGATGAC TGATGTATTT 
GGGAATTATG TCATCCAAAA GTTCTTTGAG TTTGGAAGTG CAGACCAGAA ACTTGCTTTA 
GCGACCCGTA TCCGCGGACA CGTCCTTCCC CTGGCGTTGC AGATGTATGG GTGCAGGGTC 
ATTCAGAAAG CCCTGGAGTC CATTTCCTCA GACCAGCAGG TAATTAGCGA CATTGTCCGT 
GAGCTTGATG GCCATGTGCT GAAGTGTGTG AAAGACCAGA ATGGGAACCA TGTGGTACAG 
AAGTGCATTG AATGTGTCCA GCCTCAGGCC CTTCAGTTCA TCATTGATGC CTTCCAGGGA 
CAGGTTTTTG TACTTTCCAC ACACCCCTAT GGCTGCAGAG TTATCCAAAG GATCTTGGAG 
CACTGCACCC AGGAGCAAAC TCTGCCCATC CTGGAAGAGC TTCATCAGCA CTCTGAACAG 
CTGGGCCAGA AATATCAAGG CGTATCATTG GAGATGACAC CCAAAACATA TTATACAGTG 
TCCCGTGATG CACTGTTCAA GGATCAGTAC GGTAACTACG TCATTCAGCA TGTTTTGGAG 
CATGGCAGAC CAGAAGATAA GAGCAAGATA GTGGCAGAGG TGCGCGGGAA GGTTCTTGTC 
CTGAGCCAGC ATAAATTTGC AAGTAATGTG GTGGAGAAGT GTGTGATCCA CTCCTCGCGT 
GCAGAGAGAG CTCTGCTGAT AGATGAAGTG TGCTGCCAGA AGGACGGGCC CCACAGCGCC 
TTGTACACCA TGATGAAAGA CCAGTACGCC AACTATGTTG TCCAAAGAAT GATCGACATG 
GCAGAACCTG CTCAGCGTAA AATCATCATG CACAAGATCC GGCCTCACAT TGCCACTTTG 
CGTAAGTACA CCTACGGGAA GCACATTCTA GCCAAGCTAG AGAAGTACTA CATGAAGAGT 
GGATCTGAAC TGGGTCCAAT CGGCGGTCCC ACGAATGGTC TGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005475018.1
CDS	446..4033
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQTAALGAFSDQSSVVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSQGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSRDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_005474961.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005474961.3

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GACTGCTGCA 
CTCGGGGCTT TCAGTGATCA AAGCAGCGTA GTCCGTGCAG TGGATGAAAT GAACTATGAT 
TTTCAAGCTC TTGCTTTGGA GTCGAGGGGG ATGGGAGAGC TTTTGCCAGC AAAAAAGCTC 
TGGGATTCTG ATGAGCTGGC CAAAGATGGC AGGAAAGGAA TGCTTCTTGG AGAAGAGTGG 
AGAGACAATG CATGGGGATC ATCTCAAGGC GGAGGTCTCG GCGTGAGCAT GGTGGAGTAC 
GTCCTGAGTT CGTCCCCTGG TGACAAGATG GATGGCCGCT ACAGGAATGG TGGCTATGGT 
GCAGGAGATG CTGACCAAGA TGGGAGAGAG AAAAGTGATG CGCAAGAGAA GGTGTCGCCT 
TTTGAAGAGG ACAAAAGCCC TGAGATGAAG GTGGGAGAGG AGAGTGATCC TACTAAAGCC 
AATGGAAGAG GTCTGCTGAA CGGCATGGAC AGAGACTGTA AAGACTTCAA CCCAACTCCT 
GGAAGCCGTC AAGCTTCTCC CACTGAGGCT GTGGAGAGGA TGGGTCCCAG TCAGACAGGT 
TTGGAGATGA TGGGGCAGCA CCACCACCCT CATGTTCTCC AACAGCAGAA CCCCTCCCAA 
AACAAGGTCC AAGCTGAGGA TTTCCAGAAC CAGGAGGCCC AGAACATGGG CGGTATGGAG 
CAGCAAGCTG GTGTGGAGTC CTTACAGTTT GACTATGCTG GTAATCAGAT TCAGGTGGAC 
TCTTCAGGGA CTCCAGTTGG ATTGTTTGAC TACAACTCTC AGCAGCAGTT GTTCCAGAGG 
TCTAATCCAT TGACTGTTCA ACAGCTCACT GCAGCTCAGC AGCAACAATA TGCACTGGCT 
GCAGCCCAGC AGCAGCATCT TGCTGGCCTT GCTCCTGCGT TTGTGCCAAA CCCTTACATC 
ATTAATGCTG CCCCACCTGG AGCTGATCCC TACACTGCTG CTGGGTTGGC AGCAGCAGCA 
ACGCTTGCAG GCCCCACAGT TGTTCCACCA CAGTACTACG GCGTCCCTTG GGGTGTGTAC 
CCAGCTAATC TTTTCCAGCA GCAGCCTGCA TCTACTGCCA ATCACTCGGC CAATCAGCAA 
GCATCCAGTC AAGGGCCAGG GCCAGGGCAA CCACAGGTGA TGCGCACTGG AACCAACCAG 
CGACCTCTTA CACCTGGGCA AGGCCAGCAG AGCCAGCAGG AATCCCTAGC TGCAGCAGCT 
GCTGCAAACC CTGCATTGGC ATACACAGGA ATGCCTGGTT ATCAGGTTTT GGCCCCTGCA 
GCTTACTATG ACCAGACTGG AGCTTTGGTA ATGGGCCCTG GTGCCCGCAC CGGTCTGGGT 
GGGCCTGTTC GTCTAGTCCA AACCCCACTT CTTATCAACC ATGCAGCGGC GCAGGCTGCA 
GCAGCAGTGT CTGCATCTGG CTCCGGTAAC AACATGTCTG GTCCTCCAGC CAACGGACTG 
TACCGCTCCA TGCCTCAGGC TCAACCTCAG CCGCAGCAGC AGCAGGCTCC TCCACCCAGC 
AGCGGCCTGC CCTCCAGCTC ATTCTACGGA TCTGGATCAG TCCCTAACAC CTCTCAGAGC 
AGCTCCCTTT TCTCTCACAC CTCTGCTGCC CCACCACCCC CAAGCTCTTC CCTGGGCTTC 
AGCAGCACCG GCGGCTCTCT TGGCGTTGGC CTGGGCTCTG CTCTTGGGGG TTTTGGCTCT 
TCTGTATCCA GCTCGACCAG TAGCAGTGTA TCCCGCAGGG ATTCCCTGTT GGCAAGTTCT 
GACCTATACA AACGAGGTGG CAGCAGTTTA ACTCCCATTG GGCAGCCGTT TTATAACAGC 
CTGGGTTACT CCTCTTCACC TAGCCCCATT GGCCTTACAC CAGGTCATTC CCCACTCACT 
CCTCCGCCAT CTTTGCCCTC TTCCCATGGA TCCTCTTCCA GCCTTCACCT AGGTGGCCTG 
ACAAATGGCA GTGGTCGTTA CATTTCTGCA GCTCCCGGAG CTGAGGCCAA ATACCGGAGC 
ACAGGCAGCA CGTCCAGTCT GTTCAATTCC AGCAGCCAGT TGTTCCCTCC CTCACGGCCC 
CGCTACAGCC GCTCTGATGT CATGCCATCT GGACGCAGCC GCCTATTGGA AGACTTCAGG 
AACAACCGTT TTCCAAACCT CCAGCTCCGT GACTTGCCTG GACACATGGT GGAGTTCTCT 
CAAGACCAGC ACGGATCCAG ATTTATCCAG CAGAAGCTAG AGAGGGCCAC TCCTGCTGAG 
AGACAGATGG TGTTTGGAGA GATTTTGCAA GCAGCATACC AACTGATGAC TGATGTATTT 
GGGAATTATG TCATCCAAAA GTTCTTTGAG TTTGGAAGTG CAGACCAGAA ACTTGCTTTA 
GCGACCCGTA TCCGCGGACA CGTCCTTCCC CTGGCGTTGC AGATGTATGG GTGCAGGGTC 
ATTCAGAAAG CCCTGGAGTC CATTTCCTCA GACCAGCAGG TAATTAGCGA CATTGTCCGT 
GAGCTTGATG GCCATGTGCT GAAGTGTGTG AAAGACCAGA ATGGGAACCA TGTGGTACAG 
AAGTGCATTG AATGTGTCCA GCCTCAGGCC CTTCAGTTCA TCATTGATGC CTTCCAGGGA 
CAGGTTTTTG TACTTTCCAC ACACCCCTAT GGCTGCAGAG TTATCCAAAG GATCTTGGAG 
CACTGCACCC AGGAGCAAAC TCTGCCCATC CTGGAAGAGC TTCATCAGCA CTCTGAACAG 
CTGGGCCAGA AATATCAAGG CGTATCATTG GAGATGACAC CCAAAACATA TTATACAGTG 
TCCCGTGATG CACTGTTCAA GGATCAGTAC GGTAACTACG TCATTCAGCA TGTTTTGGAG 
CATGGCAGAC CAGAAGATAA GAGCAAGATA GTGGCAGAGG TGCGCGGGAA GGTTCTTGTC 
CTGAGCCAGC ATAAATTTGC AAGTAATGTG GTGGAGAAGT GTGTGATCCA CTCCTCGCGT 
GCAGAGAGAG CTCTGCTGAT AGATGAAGTG TGCTGCCAGA AGGACGGGCC CCACAGCGCC 
TTGTACACCA TGATGAAAGA CCAGTACGCC AACTATGTTG TCCAAAGAAT GATCGACATG 
GCAGAACCTG CTCAGCGTAA AATCATCATG CACAAGATCC GGCCTCACAT TGCCACTTTG 
CGTAAGTACA CCTACGGGAA GCACATTCTA GCCAAGCTAG AGAAGTACTA CATGAAGAGT 
GGATCTGAAC TGGGTCCAAT CGGCGGTCCC ACGAATGGTC TGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_003446310.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003446358.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X8, mRNA.

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CloneID	OOr40021
Clone ID Related Accession (Same CDS sequence)	XM_003446310.4 , XM_003446310.5
Accession Version	XM_003446310.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_003446310.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGAGCG ACATTGTCCG TGAGCTTGAT 
GGCCATGTGC TGAAGTGTGT GAAAGACCAG AATGGGAACC ATGTGGTACA GAAGTGCATT 
GAATGTGTCC AGCCTCAGGC CCTTCAGTTC ATCATTGATG CCTTCCAGGG ACAGGTTTTT 
GTACTTTCCA CACACCCCTA TGGCTGCAGA GTTATCCAAA GGATCTTGGA GCACTGCACC 
CAGGAGCAAA CTCTGCCCAT CCTGGAAGAG CTTCATCAGC ACTCTGAACA GCTGGGCCAG 
GATCAGTACG GTAACTACGT CATTCAGCAT GTTTTGGAGC ATGGCAGACC AGAAGATAAG 
AGCAAGATAG TGGCAGAGGT GCGCGGGAAG GTTCTTGTCC TGAGCCAGCA TAAATTTGCA 
AGTAATGTGG TGGAGAAGTG TGTGATCCAC TCCTCGCGTG CAGAGAGAGC TCTGCTGATA 
GATGAAGTGT GCTGCCAGAA GGACGGGCCC CACAGCGCCT TGTACACCAT GATGAAAGAC 
CAGTACGCCA ACTATGTTGT CCAAAGAATG ATCGACATGG CAGAACCTGC TCAGCGTAAA 
ATCATCATGC ACAAGATCCG GCCTCACATT GCCACTTTGC GTAAGTACAC CTACGGGAAG 
CACATTCTAG CCAAGCTAGA GAAGTACTAC ATGAAGAGTG GATCTGAACT GGGTCCAATC 
GGCGGTCCCA CGAATGGTCT GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003446358.1
CDS	446..4012
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_003446310.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003446310.4

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGAGCG ACATTGTCCG TGAGCTTGAT 
GGCCATGTGC TGAAGTGTGT GAAAGACCAG AATGGGAACC ATGTGGTACA GAAGTGCATT 
GAATGTGTCC AGCCTCAGGC CCTTCAGTTC ATCATTGATG CCTTCCAGGG ACAGGTTTTT 
GTACTTTCCA CACACCCCTA TGGCTGCAGA GTTATCCAAA GGATCTTGGA GCACTGCACC 
CAGGAGCAAA CTCTGCCCAT CCTGGAAGAG CTTCATCAGC ACTCTGAACA GCTGGGCCAG 
GATCAGTACG GTAACTACGT CATTCAGCAT GTTTTGGAGC ATGGCAGACC AGAAGATAAG 
AGCAAGATAG TGGCAGAGGT GCGCGGGAAG GTTCTTGTCC TGAGCCAGCA TAAATTTGCA 
AGTAATGTGG TGGAGAAGTG TGTGATCCAC TCCTCGCGTG CAGAGAGAGC TCTGCTGATA 
GATGAAGTGT GCTGCCAGAA GGACGGGCCC CACAGCGCCT TGTACACCAT GATGAAAGAC 
CAGTACGCCA ACTATGTTGT CCAAAGAATG ATCGACATGG CAGAACCTGC TCAGCGTAAA 
ATCATCATGC ACAAGATCCG GCCTCACATT GCCACTTTGC GTAAGTACAC CTACGGGAAG 
CACATTCTAG CCAAGCTAGA GAAGTACTAC ATGAAGAGTG GATCTGAACT GGGTCCAATC 
GGCGGTCCCA CGAATGGTCT GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_003446310.5 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003446358.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OOr40021
Clone ID Related Accession (Same CDS sequence)	XM_003446310.4 , XM_003446310.5
Accession Version	XM_003446310.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_003446310.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGAGCG ACATTGTCCG TGAGCTTGAT 
GGCCATGTGC TGAAGTGTGT GAAAGACCAG AATGGGAACC ATGTGGTACA GAAGTGCATT 
GAATGTGTCC AGCCTCAGGC CCTTCAGTTC ATCATTGATG CCTTCCAGGG ACAGGTTTTT 
GTACTTTCCA CACACCCCTA TGGCTGCAGA GTTATCCAAA GGATCTTGGA GCACTGCACC 
CAGGAGCAAA CTCTGCCCAT CCTGGAAGAG CTTCATCAGC ACTCTGAACA GCTGGGCCAG 
GATCAGTACG GTAACTACGT CATTCAGCAT GTTTTGGAGC ATGGCAGACC AGAAGATAAG 
AGCAAGATAG TGGCAGAGGT GCGCGGGAAG GTTCTTGTCC TGAGCCAGCA TAAATTTGCA 
AGTAATGTGG TGGAGAAGTG TGTGATCCAC TCCTCGCGTG CAGAGAGAGC TCTGCTGATA 
GATGAAGTGT GCTGCCAGAA GGACGGGCCC CACAGCGCCT TGTACACCAT GATGAAAGAC 
CAGTACGCCA ACTATGTTGT CCAAAGAATG ATCGACATGG CAGAACCTGC TCAGCGTAAA 
ATCATCATGC ACAAGATCCG GCCTCACATT GCCACTTTGC GTAAGTACAC CTACGGGAAG 
CACATTCTAG CCAAGCTAGA GAAGTACTAC ATGAAGAGTG GATCTGAACT GGGTCCAATC 
GGCGGTCCCA CGAATGGTCT GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003446358.1
CDS	486..4052
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_003446310.5
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003446310.5

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGAGCG ACATTGTCCG TGAGCTTGAT 
GGCCATGTGC TGAAGTGTGT GAAAGACCAG AATGGGAACC ATGTGGTACA GAAGTGCATT 
GAATGTGTCC AGCCTCAGGC CCTTCAGTTC ATCATTGATG CCTTCCAGGG ACAGGTTTTT 
GTACTTTCCA CACACCCCTA TGGCTGCAGA GTTATCCAAA GGATCTTGGA GCACTGCACC 
CAGGAGCAAA CTCTGCCCAT CCTGGAAGAG CTTCATCAGC ACTCTGAACA GCTGGGCCAG 
GATCAGTACG GTAACTACGT CATTCAGCAT GTTTTGGAGC ATGGCAGACC AGAAGATAAG 
AGCAAGATAG TGGCAGAGGT GCGCGGGAAG GTTCTTGTCC TGAGCCAGCA TAAATTTGCA 
AGTAATGTGG TGGAGAAGTG TGTGATCCAC TCCTCGCGTG CAGAGAGAGC TCTGCTGATA 
GATGAAGTGT GCTGCCAGAA GGACGGGCCC CACAGCGCCT TGTACACCAT GATGAAAGAC 
CAGTACGCCA ACTATGTTGT CCAAAGAATG ATCGACATGG CAGAACCTGC TCAGCGTAAA 
ATCATCATGC ACAAGATCCG GCCTCACATT GCCACTTTGC GTAAGTACAC CTACGGGAAG 
CACATTCTAG CCAAGCTAGA GAAGTACTAC ATGAAGAGTG GATCTGAACT GGGTCCAATC 
GGCGGTCCCA CGAATGGTCT GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_005474962.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005475019.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X9, mRNA.

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CloneID	OOr40022
Clone ID Related Accession (Same CDS sequence)	XM_005474962.4 , XM_005474962.3
Accession Version	XM_005474962.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_005474962.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGGTAA TTAGCGACAT TGTCCGTGAG 
CTTGATGGCC ATGTGCTGAA GTGTGTGAAA GACCAGAATG GGAACCATGT GGTACAGAAG 
TGCATTGAAT GTGTCCAGCC TCAGGCCCTT CAGTTCATCA TTGATGCCTT CCAGGGACAG 
GTTTTTGTAC TTTCCACACA CCCCTATGGC TGCAGAGTTA TCCAAAGGAT CTTGGAGCAC 
TGCACCCAGG AGCAAACTCT GCCCATCCTG GAAGAGCTTC ATCAGCACTC TGAACAGCTG 
GGCCAGGATC AGTACGGTAA CTACGTCATT CAGCATGTTT TGGAGCATGG CAGACCAGAA 
GATAAGAGCA AGATAGTGGC AGAGGTGCGC GGGAAGGTTC TTGTCCTGAG CCAGCATAAA 
TTTGCAAGTA ATGTGGTGGA GAAGTGTGTG ATCCACTCCT CGCGTGCAGA GAGAGCTCTG 
CTGATAGATG AAGTGTGCTG CCAGAAGGAC GGGCCCCACA GCGCCTTGTA CACCATGATG 
AAAGACCAGT ACGCCAACTA TGTTGTCCAA AGAATGATCG ACATGGCAGA ACCTGCTCAG 
CGTAAAATCA TCATGCACAA GATCCGGCCT CACATTGCCA CTTTGCGTAA GTACACCTAC 
GGGAAGCACA TTCTAGCCAA GCTAGAGAAG TACTACATGA AGAGTGGATC TGAACTGGGT 
CCAATCGGCG GTCCCACGAA TGGTCTGATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005475019.1
CDS	486..4058
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_005474962.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005474962.4

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGGTAA TTAGCGACAT TGTCCGTGAG 
CTTGATGGCC ATGTGCTGAA GTGTGTGAAA GACCAGAATG GGAACCATGT GGTACAGAAG 
TGCATTGAAT GTGTCCAGCC TCAGGCCCTT CAGTTCATCA TTGATGCCTT CCAGGGACAG 
GTTTTTGTAC TTTCCACACA CCCCTATGGC TGCAGAGTTA TCCAAAGGAT CTTGGAGCAC 
TGCACCCAGG AGCAAACTCT GCCCATCCTG GAAGAGCTTC ATCAGCACTC TGAACAGCTG 
GGCCAGGATC AGTACGGTAA CTACGTCATT CAGCATGTTT TGGAGCATGG CAGACCAGAA 
GATAAGAGCA AGATAGTGGC AGAGGTGCGC GGGAAGGTTC TTGTCCTGAG CCAGCATAAA 
TTTGCAAGTA ATGTGGTGGA GAAGTGTGTG ATCCACTCCT CGCGTGCAGA GAGAGCTCTG 
CTGATAGATG AAGTGTGCTG CCAGAAGGAC GGGCCCCACA GCGCCTTGTA CACCATGATG 
AAAGACCAGT ACGCCAACTA TGTTGTCCAA AGAATGATCG ACATGGCAGA ACCTGCTCAG 
CGTAAAATCA TCATGCACAA GATCCGGCCT CACATTGCCA CTTTGCGTAA GTACACCTAC 
GGGAAGCACA TTCTAGCCAA GCTAGAGAAG TACTACATGA AGAGTGGATC TGAACTGGGT 
CCAATCGGCG GTCCCACGAA TGGTCTGATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum2 ( XM_005474962.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005475019.1 Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OOr40022
Clone ID Related Accession (Same CDS sequence)	XM_005474962.4 , XM_005474962.3
Accession Version	XM_005474962.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	pumilio homolog 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_005474962.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGGTAA TTAGCGACAT TGTCCGTGAG 
CTTGATGGCC ATGTGCTGAA GTGTGTGAAA GACCAGAATG GGAACCATGT GGTACAGAAG 
TGCATTGAAT GTGTCCAGCC TCAGGCCCTT CAGTTCATCA TTGATGCCTT CCAGGGACAG 
GTTTTTGTAC TTTCCACACA CCCCTATGGC TGCAGAGTTA TCCAAAGGAT CTTGGAGCAC 
TGCACCCAGG AGCAAACTCT GCCCATCCTG GAAGAGCTTC ATCAGCACTC TGAACAGCTG 
GGCCAGGATC AGTACGGTAA CTACGTCATT CAGCATGTTT TGGAGCATGG CAGACCAGAA 
GATAAGAGCA AGATAGTGGC AGAGGTGCGC GGGAAGGTTC TTGTCCTGAG CCAGCATAAA 
TTTGCAAGTA ATGTGGTGGA GAAGTGTGTG ATCCACTCCT CGCGTGCAGA GAGAGCTCTG 
CTGATAGATG AAGTGTGCTG CCAGAAGGAC GGGCCCCACA GCGCCTTGTA CACCATGATG 
AAAGACCAGT ACGCCAACTA TGTTGTCCAA AGAATGATCG ACATGGCAGA ACCTGCTCAG 
CGTAAAATCA TCATGCACAA GATCCGGCCT CACATTGCCA CTTTGCGTAA GTACACCTAC 
GGGAAGCACA TTCTAGCCAA GCTAGAGAAG TACTACATGA AGAGTGGATC TGAACTGGGT 
CCAATCGGCG GTCCCACGAA TGGTCTGATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005475019.1
CDS	446..4018
Translation	MSIPCSILGMNDVAWQETRGGMLHANGAPESGGVRVHGGGPLATVGGAGQAPGGPHIQGMDRVPNPTPGTPQPPLSGRSQDDATVGYFFQRQPGEQLVSCTPSKHRWPTGDANHVDQVRAVDEMNYDFQALALESRGMGELLPAKKLWDSDELAKDGRKGMLLGEEWRDNAWGSSHHSVSQPIMVQRRPGQGFHGNGDANSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGAGDADQDGREKSDAQEKVSPFEEDKSPEMKVGEESDPTKANGRGLLNGMDRDCKDFNPTPGSRQASPTEAVERMGPSQTGLEMMGQHHHPHVLQQQNPSQNKVQAEDFQNQEAQNMGGMEQQAGVESLQFDYAGNQIQVDSSGTPVGLFDYNSQQQLFQRSNPLTVQQLTAAQQQQYALAAAQQQHLAGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPTVVPPQYYGVPWGVYPANLFQQQPASTANHSANQQASSQGPGPGQPQVMRTGTNQRPLTPGQGQQSQQESLAAAAAANPALAYTGMPGYQVLAPAAYYDQTGALVMGPGARTGLGGPVRLVQTPLLINHAAAQAAAAVSASGSGNNMSGPPANGLYRSMPQAQPQPQQQQAPPPSSGLPSSSFYGSGSVPNTSQSSSLFSHTSAAPPPPSSSLGFSSTGGSLGVGLGSALGGFGSSVSSSTSSSVSRRDSLLASSDLYKRGGSSLTPIGQPFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSTGSTSSLFNSSSQLFPPSRPRYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATPAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVQKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLVLSQHKFASNVVEKCVIHSSRAERALLIDEVCCQKDGPHSALYTMMKDQYANYVVQRMIDMAEPAQRKIIMHKIRPHIATLRKYTYGKHILAKLEKYYMKSGSELGPIGGPTNGLM

Target ORF information:

RefSeq Version	XM_005474962.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus pumilio RNA binding family member 2 (pum2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005474962.3

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTGG CCTGGCAGGA GACAAGAGGT 
GGGATGCTGC ATGCAAACGG TGCTCCTGAG TCTGGGGGTG TCCGAGTTCA TGGCGGAGGG 
CCCCTAGCCA CAGTTGGGGG AGCTGGTCAG GCTCCTGGAG GGCCACATAT ACAGGGCATG 
GACAGGGTTC CTAACCCTAC CCCAGGTACC CCACAGCCCC CGCTGAGCGG GAGGTCTCAG 
GATGACGCCA CAGTAGGATA CTTCTTTCAG AGGCAGCCTG GGGAGCAGCT TGTGAGTTGC 
ACACCTAGCA AGCATCGCTG GCCTACTGGG GATGCCAATC ATGTCGATCA GGTCCGTGCA 
GTGGATGAAA TGAACTATGA TTTTCAAGCT CTTGCTTTGG AGTCGAGGGG GATGGGAGAG 
CTTTTGCCAG CAAAAAAGCT CTGGGATTCT GATGAGCTGG CCAAAGATGG CAGGAAAGGA 
ATGCTTCTTG GAGAAGAGTG GAGAGACAAT GCATGGGGAT CATCTCATCA TTCAGTGTCT 
CAGCCAATCA TGGTGCAGCG ACGGCCAGGC CAGGGTTTCC ATGGGAATGG TGATGCCAAT 
TCTGTGCTTT CACCTCGCTC AGAAGGCGGA GGTCTCGGCG TGAGCATGGT GGAGTACGTC 
CTGAGTTCGT CCCCTGGTGA CAAGATGGAT GGCCGCTACA GGAATGGTGG CTATGGTGCA 
GGAGATGCTG ACCAAGATGG GAGAGAGAAA AGTGATGCGC AAGAGAAGGT GTCGCCTTTT 
GAAGAGGACA AAAGCCCTGA GATGAAGGTG GGAGAGGAGA GTGATCCTAC TAAAGCCAAT 
GGAAGAGGTC TGCTGAACGG CATGGACAGA GACTGTAAAG ACTTCAACCC AACTCCTGGA 
AGCCGTCAAG CTTCTCCCAC TGAGGCTGTG GAGAGGATGG GTCCCAGTCA GACAGGTTTG 
GAGATGATGG GGCAGCACCA CCACCCTCAT GTTCTCCAAC AGCAGAACCC CTCCCAAAAC 
AAGGTCCAAG CTGAGGATTT CCAGAACCAG GAGGCCCAGA ACATGGGCGG TATGGAGCAG 
CAAGCTGGTG TGGAGTCCTT ACAGTTTGAC TATGCTGGTA ATCAGATTCA GGTGGACTCT 
TCAGGGACTC CAGTTGGATT GTTTGACTAC AACTCTCAGC AGCAGTTGTT CCAGAGGTCT 
AATCCATTGA CTGTTCAACA GCTCACTGCA GCTCAGCAGC AACAATATGC ACTGGCTGCA 
GCCCAGCAGC AGCATCTTGC TGGCCTTGCT CCTGCGTTTG TGCCAAACCC TTACATCATT 
AATGCTGCCC CACCTGGAGC TGATCCCTAC ACTGCTGCTG GGTTGGCAGC AGCAGCAACG 
CTTGCAGGCC CCACAGTTGT TCCACCACAG TACTACGGCG TCCCTTGGGG TGTGTACCCA 
GCTAATCTTT TCCAGCAGCA GCCTGCATCT ACTGCCAATC ACTCGGCCAA TCAGCAAGCA 
TCCAGTCAAG GGCCAGGGCC AGGGCAACCA CAGGTGATGC GCACTGGAAC CAACCAGCGA 
CCTCTTACAC CTGGGCAAGG CCAGCAGAGC CAGCAGGAAT CCCTAGCTGC AGCAGCTGCT 
GCAAACCCTG CATTGGCATA CACAGGAATG CCTGGTTATC AGGTTTTGGC CCCTGCAGCT 
TACTATGACC AGACTGGAGC TTTGGTAATG GGCCCTGGTG CCCGCACCGG TCTGGGTGGG 
CCTGTTCGTC TAGTCCAAAC CCCACTTCTT ATCAACCATG CAGCGGCGCA GGCTGCAGCA 
GCAGTGTCTG CATCTGGCTC CGGTAACAAC ATGTCTGGTC CTCCAGCCAA CGGACTGTAC 
CGCTCCATGC CTCAGGCTCA ACCTCAGCCG CAGCAGCAGC AGGCTCCTCC ACCCAGCAGC 
GGCCTGCCCT CCAGCTCATT CTACGGATCT GGATCAGTCC CTAACACCTC TCAGAGCAGC 
TCCCTTTTCT CTCACACCTC TGCTGCCCCA CCACCCCCAA GCTCTTCCCT GGGCTTCAGC 
AGCACCGGCG GCTCTCTTGG CGTTGGCCTG GGCTCTGCTC TTGGGGGTTT TGGCTCTTCT 
GTATCCAGCT CGACCAGTAG CAGTGTATCC CGCAGGGATT CCCTGTTGGC AAGTTCTGAC 
CTATACAAAC GAGGTGGCAG CAGTTTAACT CCCATTGGGC AGCCGTTTTA TAACAGCCTG 
GGTTACTCCT CTTCACCTAG CCCCATTGGC CTTACACCAG GTCATTCCCC ACTCACTCCT 
CCGCCATCTT TGCCCTCTTC CCATGGATCC TCTTCCAGCC TTCACCTAGG TGGCCTGACA 
AATGGCAGTG GTCGTTACAT TTCTGCAGCT CCCGGAGCTG AGGCCAAATA CCGGAGCACA 
GGCAGCACGT CCAGTCTGTT CAATTCCAGC AGCCAGTTGT TCCCTCCCTC ACGGCCCCGC 
TACAGCCGCT CTGATGTCAT GCCATCTGGA CGCAGCCGCC TATTGGAAGA CTTCAGGAAC 
AACCGTTTTC CAAACCTCCA GCTCCGTGAC TTGCCTGGAC ACATGGTGGA GTTCTCTCAA 
GACCAGCACG GATCCAGATT TATCCAGCAG AAGCTAGAGA GGGCCACTCC TGCTGAGAGA 
CAGATGGTGT TTGGAGAGAT TTTGCAAGCA GCATACCAAC TGATGACTGA TGTATTTGGG 
AATTATGTCA TCCAAAAGTT CTTTGAGTTT GGAAGTGCAG ACCAGAAACT TGCTTTAGCG 
ACCCGTATCC GCGGACACGT CCTTCCCCTG GCGTTGCAGA TGTATGGGTG CAGGGTCATT 
CAGAAAGCCC TGGAGTCCAT TTCCTCAGAC CAGCAGGTAA TTAGCGACAT TGTCCGTGAG 
CTTGATGGCC ATGTGCTGAA GTGTGTGAAA GACCAGAATG GGAACCATGT GGTACAGAAG 
TGCATTGAAT GTGTCCAGCC TCAGGCCCTT CAGTTCATCA TTGATGCCTT CCAGGGACAG 
GTTTTTGTAC TTTCCACACA CCCCTATGGC TGCAGAGTTA TCCAAAGGAT CTTGGAGCAC 
TGCACCCAGG AGCAAACTCT GCCCATCCTG GAAGAGCTTC ATCAGCACTC TGAACAGCTG 
GGCCAGGATC AGTACGGTAA CTACGTCATT CAGCATGTTT TGGAGCATGG CAGACCAGAA 
GATAAGAGCA AGATAGTGGC AGAGGTGCGC GGGAAGGTTC TTGTCCTGAG CCAGCATAAA 
TTTGCAAGTA ATGTGGTGGA GAAGTGTGTG ATCCACTCCT CGCGTGCAGA GAGAGCTCTG 
CTGATAGATG AAGTGTGCTG CCAGAAGGAC GGGCCCCACA GCGCCTTGTA CACCATGATG 
AAAGACCAGT ACGCCAACTA TGTTGTCCAA AGAATGATCG ACATGGCAGA ACCTGCTCAG 
CGTAAAATCA TCATGCACAA GATCCGGCCT CACATTGCCA CTTTGCGTAA GTACACCTAC 
GGGAAGCACA TTCTAGCCAA GCTAGAGAAG TACTACATGA AGAGTGGATC TGAACTGGGT 
CCAATCGGCG GTCCCACGAA TGGTCTGATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	pum2
Entrez Gene ID	100701094
Full Name	pumilio RNA binding family member 2
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

pum2 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene

Clones

gRNAs

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