LOC100697158 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100697158
Entrez Gene ID	100697158
Full Name	teashirt homolog 3
Synonyms	tshz3
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005471081.4	XP_005471138.1	teashirt homolog 3
XM_005471081.3	XP_005471138.1	teashirt homolog 3

Pathways from BioSystems
Gene Ontology

The following LOC100697158 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100697158 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr69488	XM_005471081.4 Latest version!	Oreochromis niloticus teashirt homolog 3 (LOC100697158), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OOr69488	XM_005471081.3	Oreochromis niloticus teashirt homolog 3 (LOC100697158), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100697158 ( XM_005471081.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005471138.1 Oreochromis niloticus teashirt homolog 3 (LOC100697158), mRNA.

Price & Availability↑

CloneID	OOr69488
Clone ID Related Accession (Same CDS sequence)	XM_005471081.4 , XM_005471081.3
Accession Version	XM_005471081.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	teashirt homolog 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_005471081.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGGA GGAAACAAGA AGCGCCGAAG CGCGCAGCAG CTTATTCTCC CGACGAGCTG 
ACAGAGCACC ATGTGGAGGA TGAGGAAGCA GAAGCGGACG ACCTGCCCTC AACCCCTCAG 
GACGACCTTC CCATGAAAGA TGAGTTTGTG GAGCAAAGTG TAGAGACTGC CAAGGAGCAG 
GCATGTGACC AGGAATCAGC TGAGACCACA GAGCTCTCAG GACAAGAGAT GGACAGTGAG 
TCACATGTGA GTGAGACTAG TGACCGTCTT TCAGACTTCG AGAGCCCCTC CAGAAAAGTT 
GAGGGCAGCG TTGTCACAGC ATCTATGAAT GCTGACAGCA AGACGCCTCC CATAGGCATG 
GACACGTTAG AACAAATGAA GGCTATCTAC TCCAGCTTTC TCACCAGCCC CTTGTGGTCA 
CCACTAAATT TTAACTCCAC ACCTCTACAG GCGCAGTCTT CGACTTCTGC AGAGAAACCA 
GCTCGCAGTA ACAGCACCAG TAGCAGTAGT AGCTGTAGCA GTGGTAGCTA TGACTGGCAC 
CAAACTGCAG TGGCAAAGAC ACTACAGCAG CATCCTCCAC AGAGCCGTCA CCCCGCTCAG 
TCAGAGCCCA GCCTCTTTAG TACAGTCCAA CTCCACAGGC AAAACCCAAA GCTGTTTGGC 
TCAATCTTTA CTGGGGCCAG TAAATTCCGT TGTAAGGGCT GTAGCGCTGC TTATGACACT 
CTGGTGGAGC TGACAGTTCA CATGAATGAT ACAGGCCACT ACCGTGATGA CAACCAGGAC 
AAGGCAGGCA GTGGCGCCAA GCGCTGGTCT AAACCACGTA AGCGGTCCCT GTTGGAGATG 
GAGGGAAAAG AGGATGCCCA GAAAGTTTTG AAATGCATGT ATTGTGGCCA TTCCTTTGAA 
TCCCTCCAGG ACCTCAGTGT CCACATGATC AAGACCAAAC ACTACCAGAA AGTGCCTTTG 
AAGGAGCCCA TGGCCCCTGT GGCAGCCAAA ATCATGTCTT CAAAGAAGCG GGGACTCGTG 
GGGTTAGACC TCAGTGCTAC ACCGCGCTCT AGAGAAGGAA CCCCTAAAGC TAAACACCCA 
CAAGGAGAAC TTAGTGAATC CTCACAGAAA CCTTCCTCCA GCCCATACAC TACCCCAAAT 
AACCGCTATG GACACCAGAA TGGTGCTAGC TATGCTTGGC AATTTGAATC CAACAAATTT 
CAGATCCTCA AGTGCATGGA GTGTGGGAGT TCCCATAATA CACTCCAAGA GCTGAGGACC 
CACATGATGG TGACAGGACA CTTCCTGAGG GTGACCAGCT CTGTGGGAAA GAGAATCAAA 
ACACTTCCCG AGGCTACCTC CCCCAATCCC GGGAGAGTTA CCACACCTAC AGAACAGAGG 
GTCCAGTCTG TACCTCTTGC ACCCTCTACC TTCTCCCCTC CGCCTCTTCA AACTCAACTT 
ACCACAACTC CCCCAGCCAC CTCCCCTCCC CTCAGAGAGA TCAAGAAAGA GGAGGTTGAG 
GAGGAATGCA CTAAGCAGGA GGCAGTAGGA AATGAAAAGC AAGTTGCAGT TACTGTTAGT 
AAGGAGGAAG ATACTGAGAG GGAGGAAAAA TACGACATTT CGAAATATAA CTACCTTACC 
GAAGAGGACC TGAAGGAAAG CCCTAAAGGG GGATTGGATA TTCTGAAATC ACTGGAAAAC 
ACTGTGACAT CAGCCATCAA TAAGGCACAG AGTGGCAATC CAAGCTGGGG AGGCTATCCT 
AGCATCCATG CTGCCTACCA GTTCCCCAGT GCCCTTAAGC AAGGAAGCAT GGAAAAGAAT 
TCACAAATGA AGTTCTTGTT CAATGGAGGC GATGGAGCGC CGTCCTCCCT TGCCAAGAAC 
CAGCCCCTCA TTTCCCCACC ACTTAGTCAG TCGTCACTTT TTCCCAGCAA CAACTTTCAG 
GCAATGGAAG ACTTAGTGAA AAAAGTGACT GAGAAAGTAG CAAAAGTAGA ACAAAGAGTT 
AAGCAGTTAT CTCCAAAGAG GGAGAACCAG TTCTCCCCCT GCAGCAGTGA GGCTGGAGAA 
TCACACAAGG GAGGGGAGGC AGACTCGCCT CGGGAATGGA GGGAAGTTAC CCCAACCAAT 
AGCGACAGGG GAAGCCATGG TGACAGAGCA TCTCCAGCAA CCGAACCCAA GAGAGAAGCA 
GCAGTGAAAT CCCCGCTTGC CTCTACACTG AGATGCAGCA CCGCCATTAT CACTGGTCAC 
ACTCCTCCAG AGCAGCCCTT TGTCAACCCT CTAAGTGCTC TTCAGTCAGT AATGAACATT 
CACTTGGGGA AAGCAGCCAA GCCCTCTTTG CCAAACCAAG ATCCCTTGAG TCTCCTCTCT 
AGGCTCAGCC AGAGCATGGC TGAGAAGGCC GCTGTGGCTG CTCCTCCATC ACAGACCAAA 
AAGCAAGAAA GTGTAGCTAA TAACAATAGC TTTTGCCAGC CAAATGATGA CCAACCCATG 
GACCTTACAA AAGGGAAAAG TGATCGAGGG GACTCTGTAA ACTCAGCTCC TCTAACGCCA 
TCATCCACAG CCTCCTCAGT CTCCCCCATC TCTCTTGTTA CTCCTGCAAA GCTAACAGTG 
GTCTCTCCAT ACACATCCAG CAGCCCTCTA CACGAAAATG CATTGTCGGA TATCTCAGAC 
ATGCTGAGAA ACCTTACACA GTCCCACCAT GTCACAAAGT CGGCGTCACG CCCACGTGTG 
ACGGATAAAA CTGAAGTTGT GGGCTCTACC CATGACGATG ATGACGTGTC CCTGCATGGC 
CACAAACGAA AGGGTCGTCA CTCCAACTGG AACCCACAGC ACCTCCTTCT TCTGCAGGCC 
CAGTTTGCCT CAAGCTTGAG GCAGACGTCA GAGGGGAAAT ATATCATTAA TGACCTCAGC 
CCACAAGAAA GAATGCACAT ATCTCGTTTC ACGGGTCTCT CCATGACCAC CATCAGCCAC 
TGGCTGGCCA ACGTGAAGTA CCAGCTACGG AGAACGGGCA GAACGAAGTT CCTCAAGAAC 
CTAGATTCCG GTCAACCTAT ATTCTTCTGC AGTGACTGTG CCTCGCAGAT TCGAACCCCA 
GCAGCTTACG TATGCCACCT GGAAGCCCAC TTGGGGTTCA GAATCAGGGA CCTGGCCAAG 
CTGTCTCCCA AACAGACTGT CAGGGACTCT CAGACTCTCA CTGAGAAAGT AGTGCCCCTG 
GAGTCCTTTC TTCCTCCACA GTCGCAAGAT GACTGCAGTA GTAATGGGGG AGTTTATCGC 
TGCCAGCTCT GTGTCCGTAA GTTTGCCACT AAGCATGCCA TCAAGCTTCA CCTCAGCAAG 
AGCCATGGGA AGTCTCCAGA GGACCATCTG CTATACGTGT GTGAGACGGA GAAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005471138.1
CDS	364..3723
Translation	MPRRKQEAPKRAAAYSPDELTEHHVEDEEAEADDLPSTPQDDLPMKDEFVEQSVETAKEQACDQESAETTELSGQEMDSESHVSETSDRLSDFESPSRKVEGSVVTASMNADSKTPPIGMDTLEQMKAIYSSFLTSPLWSPLNFNSTPLQAQSSTSAEKPARSNSTSSSSSCSSGSYDWHQTAVAKTLQQHPPQSRHPAQSEPSLFSTVQLHRQNPKLFGSIFTGASKFRCKGCSAAYDTLVELTVHMNDTGHYRDDNQDKAGSGAKRWSKPRKRSLLEMEGKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPLKEPMAPVAAKIMSSKKRGLVGLDLSATPRSREGTPKAKHPQGELSESSQKPSSSPYTTPNNRYGHQNGASYAWQFESNKFQILKCMECGSSHNTLQELRTHMMVTGHFLRVTSSVGKRIKTLPEATSPNPGRVTTPTEQRVQSVPLAPSTFSPPPLQTQLTTTPPATSPPLREIKKEEVEEECTKQEAVGNEKQVAVTVSKEEDTEREEKYDISKYNYLTEEDLKESPKGGLDILKSLENTVTSAINKAQSGNPSWGGYPSIHAAYQFPSALKQGSMEKNSQMKFLFNGGDGAPSSLAKNQPLISPPLSQSSLFPSNNFQAMEDLVKKVTEKVAKVEQRVKQLSPKRENQFSPCSSEAGESHKGGEADSPREWREVTPTNSDRGSHGDRASPATEPKREAAVKSPLASTLRCSTAIITGHTPPEQPFVNPLSALQSVMNIHLGKAAKPSLPNQDPLSLLSRLSQSMAEKAAVAAPPSQTKKQESVANNNSFCQPNDDQPMDLTKGKSDRGDSVNSAPLTPSSTASSVSPISLVTPAKLTVVSPYTSSSPLHENALSDISDMLRNLTQSHHVTKSASRPRVTDKTEVVGSTHDDDDVSLHGHKRKGRHSNWNPQHLLLLQAQFASSLRQTSEGKYIINDLSPQERMHISRFTGLSMTTISHWLANVKYQLRRTGRTKFLKNLDSGQPIFFCSDCASQIRTPAAYVCHLEAHLGFRIRDLAKLSPKQTVRDSQTLTEKVVPLESFLPPQSQDDCSSNGGVYRCQLCVRKFATKHAIKLHLSKSHGKSPEDHLLYVCETEKC

Target ORF information:

RefSeq Version	XM_005471081.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus teashirt homolog 3 (LOC100697158), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005471081.4

ORF Insert Sequence:

ATGCCACGGA GGAAACAAGA AGCGCCGAAG CGCGCAGCAG CTTATTCTCC CGACGAGCTG 
ACAGAGCACC ATGTGGAGGA TGAGGAAGCA GAAGCGGACG ACCTGCCCTC AACCCCTCAG 
GACGACCTTC CCATGAAAGA TGAGTTTGTG GAGCAAAGTG TAGAGACTGC CAAGGAGCAG 
GCATGTGACC AGGAATCAGC TGAGACCACA GAGCTCTCAG GACAAGAGAT GGACAGTGAG 
TCACATGTGA GTGAGACTAG TGACCGTCTT TCAGACTTCG AGAGCCCCTC CAGAAAAGTT 
GAGGGCAGCG TTGTCACAGC ATCTATGAAT GCTGACAGCA AGACGCCTCC CATAGGCATG 
GACACGTTAG AACAAATGAA GGCTATCTAC TCCAGCTTTC TCACCAGCCC CTTGTGGTCA 
CCACTAAATT TTAACTCCAC ACCTCTACAG GCGCAGTCTT CGACTTCTGC AGAGAAACCA 
GCTCGCAGTA ACAGCACCAG TAGCAGTAGT AGCTGTAGCA GTGGTAGCTA TGACTGGCAC 
CAAACTGCAG TGGCAAAGAC ACTACAGCAG CATCCTCCAC AGAGCCGTCA CCCCGCTCAG 
TCAGAGCCCA GCCTCTTTAG TACAGTCCAA CTCCACAGGC AAAACCCAAA GCTGTTTGGC 
TCAATCTTTA CTGGGGCCAG TAAATTCCGT TGTAAGGGCT GTAGCGCTGC TTATGACACT 
CTGGTGGAGC TGACAGTTCA CATGAATGAT ACAGGCCACT ACCGTGATGA CAACCAGGAC 
AAGGCAGGCA GTGGCGCCAA GCGCTGGTCT AAACCACGTA AGCGGTCCCT GTTGGAGATG 
GAGGGAAAAG AGGATGCCCA GAAAGTTTTG AAATGCATGT ATTGTGGCCA TTCCTTTGAA 
TCCCTCCAGG ACCTCAGTGT CCACATGATC AAGACCAAAC ACTACCAGAA AGTGCCTTTG 
AAGGAGCCCA TGGCCCCTGT GGCAGCCAAA ATCATGTCTT CAAAGAAGCG GGGACTCGTG 
GGGTTAGACC TCAGTGCTAC ACCGCGCTCT AGAGAAGGAA CCCCTAAAGC TAAACACCCA 
CAAGGAGAAC TTAGTGAATC CTCACAGAAA CCTTCCTCCA GCCCATACAC TACCCCAAAT 
AACCGCTATG GACACCAGAA TGGTGCTAGC TATGCTTGGC AATTTGAATC CAACAAATTT 
CAGATCCTCA AGTGCATGGA GTGTGGGAGT TCCCATAATA CACTCCAAGA GCTGAGGACC 
CACATGATGG TGACAGGACA CTTCCTGAGG GTGACCAGCT CTGTGGGAAA GAGAATCAAA 
ACACTTCCCG AGGCTACCTC CCCCAATCCC GGGAGAGTTA CCACACCTAC AGAACAGAGG 
GTCCAGTCTG TACCTCTTGC ACCCTCTACC TTCTCCCCTC CGCCTCTTCA AACTCAACTT 
ACCACAACTC CCCCAGCCAC CTCCCCTCCC CTCAGAGAGA TCAAGAAAGA GGAGGTTGAG 
GAGGAATGCA CTAAGCAGGA GGCAGTAGGA AATGAAAAGC AAGTTGCAGT TACTGTTAGT 
AAGGAGGAAG ATACTGAGAG GGAGGAAAAA TACGACATTT CGAAATATAA CTACCTTACC 
GAAGAGGACC TGAAGGAAAG CCCTAAAGGG GGATTGGATA TTCTGAAATC ACTGGAAAAC 
ACTGTGACAT CAGCCATCAA TAAGGCACAG AGTGGCAATC CAAGCTGGGG AGGCTATCCT 
AGCATCCATG CTGCCTACCA GTTCCCCAGT GCCCTTAAGC AAGGAAGCAT GGAAAAGAAT 
TCACAAATGA AGTTCTTGTT CAATGGAGGC GATGGAGCGC CGTCCTCCCT TGCCAAGAAC 
CAGCCCCTCA TTTCCCCACC ACTTAGTCAG TCGTCACTTT TTCCCAGCAA CAACTTTCAG 
GCAATGGAAG ACTTAGTGAA AAAAGTGACT GAGAAAGTAG CAAAAGTAGA ACAAAGAGTT 
AAGCAGTTAT CTCCAAAGAG GGAGAACCAG TTCTCCCCCT GCAGCAGTGA GGCTGGAGAA 
TCACACAAGG GAGGGGAGGC AGACTCGCCT CGGGAATGGA GGGAAGTTAC CCCAACCAAT 
AGCGACAGGG GAAGCCATGG TGACAGAGCA TCTCCAGCAA CCGAACCCAA GAGAGAAGCA 
GCAGTGAAAT CCCCGCTTGC CTCTACACTG AGATGCAGCA CCGCCATTAT CACTGGTCAC 
ACTCCTCCAG AGCAGCCCTT TGTCAACCCT CTAAGTGCTC TTCAGTCAGT AATGAACATT 
CACTTGGGGA AAGCAGCCAA GCCCTCTTTG CCAAACCAAG ATCCCTTGAG TCTCCTCTCT 
AGGCTCAGCC AGAGCATGGC TGAGAAGGCC GCTGTGGCTG CTCCTCCATC ACAGACCAAA 
AAGCAAGAAA GTGTAGCTAA TAACAATAGC TTTTGCCAGC CAAATGATGA CCAACCCATG 
GACCTTACAA AAGGGAAAAG TGATCGAGGG GACTCTGTAA ACTCAGCTCC TCTAACGCCA 
TCATCCACAG CCTCCTCAGT CTCCCCCATC TCTCTTGTTA CTCCTGCAAA GCTAACAGTG 
GTCTCTCCAT ACACATCCAG CAGCCCTCTA CACGAAAATG CATTGTCGGA TATCTCAGAC 
ATGCTGAGAA ACCTTACACA GTCCCACCAT GTCACAAAGT CGGCGTCACG CCCACGTGTG 
ACGGATAAAA CTGAAGTTGT GGGCTCTACC CATGACGATG ATGACGTGTC CCTGCATGGC 
CACAAACGAA AGGGTCGTCA CTCCAACTGG AACCCACAGC ACCTCCTTCT TCTGCAGGCC 
CAGTTTGCCT CAAGCTTGAG GCAGACGTCA GAGGGGAAAT ATATCATTAA TGACCTCAGC 
CCACAAGAAA GAATGCACAT ATCTCGTTTC ACGGGTCTCT CCATGACCAC CATCAGCCAC 
TGGCTGGCCA ACGTGAAGTA CCAGCTACGG AGAACGGGCA GAACGAAGTT CCTCAAGAAC 
CTAGATTCCG GTCAACCTAT ATTCTTCTGC AGTGACTGTG CCTCGCAGAT TCGAACCCCA 
GCAGCTTACG TATGCCACCT GGAAGCCCAC TTGGGGTTCA GAATCAGGGA CCTGGCCAAG 
CTGTCTCCCA AACAGACTGT CAGGGACTCT CAGACTCTCA CTGAGAAAGT AGTGCCCCTG 
GAGTCCTTTC TTCCTCCACA GTCGCAAGAT GACTGCAGTA GTAATGGGGG AGTTTATCGC 
TGCCAGCTCT GTGTCCGTAA GTTTGCCACT AAGCATGCCA TCAAGCTTCA CCTCAGCAAG 
AGCCATGGGA AGTCTCCAGA GGACCATCTG CTATACGTGT GTGAGACGGA GAAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100697158 ( XM_005471081.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005471138.1 Oreochromis niloticus teashirt homolog 3 (LOC100697158), mRNA.

Price & Availability↑

CloneID	OOr69488
Clone ID Related Accession (Same CDS sequence)	XM_005471081.4 , XM_005471081.3
Accession Version	XM_005471081.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	teashirt homolog 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_005471081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGGA GGAAACAAGA AGCGCCGAAG CGCGCAGCAG CTTATTCTCC CGACGAGCTG 
ACAGAGCACC ATGTGGAGGA TGAGGAAGCA GAAGCGGACG ACCTGCCCTC AACCCCTCAG 
GACGACCTTC CCATGAAAGA TGAGTTTGTG GAGCAAAGTG TAGAGACTGC CAAGGAGCAG 
GCATGTGACC AGGAATCAGC TGAGACCACA GAGCTCTCAG GACAAGAGAT GGACAGTGAG 
TCACATGTGA GTGAGACTAG TGACCGTCTT TCAGACTTCG AGAGCCCCTC CAGAAAAGTT 
GAGGGCAGCG TTGTCACAGC ATCTATGAAT GCTGACAGCA AGACGCCTCC CATAGGCATG 
GACACGTTAG AACAAATGAA GGCTATCTAC TCCAGCTTTC TCACCAGCCC CTTGTGGTCA 
CCACTAAATT TTAACTCCAC ACCTCTACAG GCGCAGTCTT CGACTTCTGC AGAGAAACCA 
GCTCGCAGTA ACAGCACCAG TAGCAGTAGT AGCTGTAGCA GTGGTAGCTA TGACTGGCAC 
CAAACTGCAG TGGCAAAGAC ACTACAGCAG CATCCTCCAC AGAGCCGTCA CCCCGCTCAG 
TCAGAGCCCA GCCTCTTTAG TACAGTCCAA CTCCACAGGC AAAACCCAAA GCTGTTTGGC 
TCAATCTTTA CTGGGGCCAG TAAATTCCGT TGTAAGGGCT GTAGCGCTGC TTATGACACT 
CTGGTGGAGC TGACAGTTCA CATGAATGAT ACAGGCCACT ACCGTGATGA CAACCAGGAC 
AAGGCAGGCA GTGGCGCCAA GCGCTGGTCT AAACCACGTA AGCGGTCCCT GTTGGAGATG 
GAGGGAAAAG AGGATGCCCA GAAAGTTTTG AAATGCATGT ATTGTGGCCA TTCCTTTGAA 
TCCCTCCAGG ACCTCAGTGT CCACATGATC AAGACCAAAC ACTACCAGAA AGTGCCTTTG 
AAGGAGCCCA TGGCCCCTGT GGCAGCCAAA ATCATGTCTT CAAAGAAGCG GGGACTCGTG 
GGGTTAGACC TCAGTGCTAC ACCGCGCTCT AGAGAAGGAA CCCCTAAAGC TAAACACCCA 
CAAGGAGAAC TTAGTGAATC CTCACAGAAA CCTTCCTCCA GCCCATACAC TACCCCAAAT 
AACCGCTATG GACACCAGAA TGGTGCTAGC TATGCTTGGC AATTTGAATC CAACAAATTT 
CAGATCCTCA AGTGCATGGA GTGTGGGAGT TCCCATAATA CACTCCAAGA GCTGAGGACC 
CACATGATGG TGACAGGACA CTTCCTGAGG GTGACCAGCT CTGTGGGAAA GAGAATCAAA 
ACACTTCCCG AGGCTACCTC CCCCAATCCC GGGAGAGTTA CCACACCTAC AGAACAGAGG 
GTCCAGTCTG TACCTCTTGC ACCCTCTACC TTCTCCCCTC CGCCTCTTCA AACTCAACTT 
ACCACAACTC CCCCAGCCAC CTCCCCTCCC CTCAGAGAGA TCAAGAAAGA GGAGGTTGAG 
GAGGAATGCA CTAAGCAGGA GGCAGTAGGA AATGAAAAGC AAGTTGCAGT TACTGTTAGT 
AAGGAGGAAG ATACTGAGAG GGAGGAAAAA TACGACATTT CGAAATATAA CTACCTTACC 
GAAGAGGACC TGAAGGAAAG CCCTAAAGGG GGATTGGATA TTCTGAAATC ACTGGAAAAC 
ACTGTGACAT CAGCCATCAA TAAGGCACAG AGTGGCAATC CAAGCTGGGG AGGCTATCCT 
AGCATCCATG CTGCCTACCA GTTCCCCAGT GCCCTTAAGC AAGGAAGCAT GGAAAAGAAT 
TCACAAATGA AGTTCTTGTT CAATGGAGGC GATGGAGCGC CGTCCTCCCT TGCCAAGAAC 
CAGCCCCTCA TTTCCCCACC ACTTAGTCAG TCGTCACTTT TTCCCAGCAA CAACTTTCAG 
GCAATGGAAG ACTTAGTGAA AAAAGTGACT GAGAAAGTAG CAAAAGTAGA ACAAAGAGTT 
AAGCAGTTAT CTCCAAAGAG GGAGAACCAG TTCTCCCCCT GCAGCAGTGA GGCTGGAGAA 
TCACACAAGG GAGGGGAGGC AGACTCGCCT CGGGAATGGA GGGAAGTTAC CCCAACCAAT 
AGCGACAGGG GAAGCCATGG TGACAGAGCA TCTCCAGCAA CCGAACCCAA GAGAGAAGCA 
GCAGTGAAAT CCCCGCTTGC CTCTACACTG AGATGCAGCA CCGCCATTAT CACTGGTCAC 
ACTCCTCCAG AGCAGCCCTT TGTCAACCCT CTAAGTGCTC TTCAGTCAGT AATGAACATT 
CACTTGGGGA AAGCAGCCAA GCCCTCTTTG CCAAACCAAG ATCCCTTGAG TCTCCTCTCT 
AGGCTCAGCC AGAGCATGGC TGAGAAGGCC GCTGTGGCTG CTCCTCCATC ACAGACCAAA 
AAGCAAGAAA GTGTAGCTAA TAACAATAGC TTTTGCCAGC CAAATGATGA CCAACCCATG 
GACCTTACAA AAGGGAAAAG TGATCGAGGG GACTCTGTAA ACTCAGCTCC TCTAACGCCA 
TCATCCACAG CCTCCTCAGT CTCCCCCATC TCTCTTGTTA CTCCTGCAAA GCTAACAGTG 
GTCTCTCCAT ACACATCCAG CAGCCCTCTA CACGAAAATG CATTGTCGGA TATCTCAGAC 
ATGCTGAGAA ACCTTACACA GTCCCACCAT GTCACAAAGT CGGCGTCACG CCCACGTGTG 
ACGGATAAAA CTGAAGTTGT GGGCTCTACC CATGACGATG ATGACGTGTC CCTGCATGGC 
CACAAACGAA AGGGTCGTCA CTCCAACTGG AACCCACAGC ACCTCCTTCT TCTGCAGGCC 
CAGTTTGCCT CAAGCTTGAG GCAGACGTCA GAGGGGAAAT ATATCATTAA TGACCTCAGC 
CCACAAGAAA GAATGCACAT ATCTCGTTTC ACGGGTCTCT CCATGACCAC CATCAGCCAC 
TGGCTGGCCA ACGTGAAGTA CCAGCTACGG AGAACGGGCA GAACGAAGTT CCTCAAGAAC 
CTAGATTCCG GTCAACCTAT ATTCTTCTGC AGTGACTGTG CCTCGCAGAT TCGAACCCCA 
GCAGCTTACG TATGCCACCT GGAAGCCCAC TTGGGGTTCA GAATCAGGGA CCTGGCCAAG 
CTGTCTCCCA AACAGACTGT CAGGGACTCT CAGACTCTCA CTGAGAAAGT AGTGCCCCTG 
GAGTCCTTTC TTCCTCCACA GTCGCAAGAT GACTGCAGTA GTAATGGGGG AGTTTATCGC 
TGCCAGCTCT GTGTCCGTAA GTTTGCCACT AAGCATGCCA TCAAGCTTCA CCTCAGCAAG 
AGCCATGGGA AGTCTCCAGA GGACCATCTG CTATACGTGT GTGAGACGGA GAAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005471138.1
CDS	360..3719
Translation	MPRRKQEAPKRAAAYSPDELTEHHVEDEEAEADDLPSTPQDDLPMKDEFVEQSVETAKEQACDQESAETTELSGQEMDSESHVSETSDRLSDFESPSRKVEGSVVTASMNADSKTPPIGMDTLEQMKAIYSSFLTSPLWSPLNFNSTPLQAQSSTSAEKPARSNSTSSSSSCSSGSYDWHQTAVAKTLQQHPPQSRHPAQSEPSLFSTVQLHRQNPKLFGSIFTGASKFRCKGCSAAYDTLVELTVHMNDTGHYRDDNQDKAGSGAKRWSKPRKRSLLEMEGKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPLKEPMAPVAAKIMSSKKRGLVGLDLSATPRSREGTPKAKHPQGELSESSQKPSSSPYTTPNNRYGHQNGASYAWQFESNKFQILKCMECGSSHNTLQELRTHMMVTGHFLRVTSSVGKRIKTLPEATSPNPGRVTTPTEQRVQSVPLAPSTFSPPPLQTQLTTTPPATSPPLREIKKEEVEEECTKQEAVGNEKQVAVTVSKEEDTEREEKYDISKYNYLTEEDLKESPKGGLDILKSLENTVTSAINKAQSGNPSWGGYPSIHAAYQFPSALKQGSMEKNSQMKFLFNGGDGAPSSLAKNQPLISPPLSQSSLFPSNNFQAMEDLVKKVTEKVAKVEQRVKQLSPKRENQFSPCSSEAGESHKGGEADSPREWREVTPTNSDRGSHGDRASPATEPKREAAVKSPLASTLRCSTAIITGHTPPEQPFVNPLSALQSVMNIHLGKAAKPSLPNQDPLSLLSRLSQSMAEKAAVAAPPSQTKKQESVANNNSFCQPNDDQPMDLTKGKSDRGDSVNSAPLTPSSTASSVSPISLVTPAKLTVVSPYTSSSPLHENALSDISDMLRNLTQSHHVTKSASRPRVTDKTEVVGSTHDDDDVSLHGHKRKGRHSNWNPQHLLLLQAQFASSLRQTSEGKYIINDLSPQERMHISRFTGLSMTTISHWLANVKYQLRRTGRTKFLKNLDSGQPIFFCSDCASQIRTPAAYVCHLEAHLGFRIRDLAKLSPKQTVRDSQTLTEKVVPLESFLPPQSQDDCSSNGGVYRCQLCVRKFATKHAIKLHLSKSHGKSPEDHLLYVCETEKC

Target ORF information:

RefSeq Version	XM_005471081.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus teashirt homolog 3 (LOC100697158), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005471081.3

ORF Insert Sequence:

ATGCCACGGA GGAAACAAGA AGCGCCGAAG CGCGCAGCAG CTTATTCTCC CGACGAGCTG 
ACAGAGCACC ATGTGGAGGA TGAGGAAGCA GAAGCGGACG ACCTGCCCTC AACCCCTCAG 
GACGACCTTC CCATGAAAGA TGAGTTTGTG GAGCAAAGTG TAGAGACTGC CAAGGAGCAG 
GCATGTGACC AGGAATCAGC TGAGACCACA GAGCTCTCAG GACAAGAGAT GGACAGTGAG 
TCACATGTGA GTGAGACTAG TGACCGTCTT TCAGACTTCG AGAGCCCCTC CAGAAAAGTT 
GAGGGCAGCG TTGTCACAGC ATCTATGAAT GCTGACAGCA AGACGCCTCC CATAGGCATG 
GACACGTTAG AACAAATGAA GGCTATCTAC TCCAGCTTTC TCACCAGCCC CTTGTGGTCA 
CCACTAAATT TTAACTCCAC ACCTCTACAG GCGCAGTCTT CGACTTCTGC AGAGAAACCA 
GCTCGCAGTA ACAGCACCAG TAGCAGTAGT AGCTGTAGCA GTGGTAGCTA TGACTGGCAC 
CAAACTGCAG TGGCAAAGAC ACTACAGCAG CATCCTCCAC AGAGCCGTCA CCCCGCTCAG 
TCAGAGCCCA GCCTCTTTAG TACAGTCCAA CTCCACAGGC AAAACCCAAA GCTGTTTGGC 
TCAATCTTTA CTGGGGCCAG TAAATTCCGT TGTAAGGGCT GTAGCGCTGC TTATGACACT 
CTGGTGGAGC TGACAGTTCA CATGAATGAT ACAGGCCACT ACCGTGATGA CAACCAGGAC 
AAGGCAGGCA GTGGCGCCAA GCGCTGGTCT AAACCACGTA AGCGGTCCCT GTTGGAGATG 
GAGGGAAAAG AGGATGCCCA GAAAGTTTTG AAATGCATGT ATTGTGGCCA TTCCTTTGAA 
TCCCTCCAGG ACCTCAGTGT CCACATGATC AAGACCAAAC ACTACCAGAA AGTGCCTTTG 
AAGGAGCCCA TGGCCCCTGT GGCAGCCAAA ATCATGTCTT CAAAGAAGCG GGGACTCGTG 
GGGTTAGACC TCAGTGCTAC ACCGCGCTCT AGAGAAGGAA CCCCTAAAGC TAAACACCCA 
CAAGGAGAAC TTAGTGAATC CTCACAGAAA CCTTCCTCCA GCCCATACAC TACCCCAAAT 
AACCGCTATG GACACCAGAA TGGTGCTAGC TATGCTTGGC AATTTGAATC CAACAAATTT 
CAGATCCTCA AGTGCATGGA GTGTGGGAGT TCCCATAATA CACTCCAAGA GCTGAGGACC 
CACATGATGG TGACAGGACA CTTCCTGAGG GTGACCAGCT CTGTGGGAAA GAGAATCAAA 
ACACTTCCCG AGGCTACCTC CCCCAATCCC GGGAGAGTTA CCACACCTAC AGAACAGAGG 
GTCCAGTCTG TACCTCTTGC ACCCTCTACC TTCTCCCCTC CGCCTCTTCA AACTCAACTT 
ACCACAACTC CCCCAGCCAC CTCCCCTCCC CTCAGAGAGA TCAAGAAAGA GGAGGTTGAG 
GAGGAATGCA CTAAGCAGGA GGCAGTAGGA AATGAAAAGC AAGTTGCAGT TACTGTTAGT 
AAGGAGGAAG ATACTGAGAG GGAGGAAAAA TACGACATTT CGAAATATAA CTACCTTACC 
GAAGAGGACC TGAAGGAAAG CCCTAAAGGG GGATTGGATA TTCTGAAATC ACTGGAAAAC 
ACTGTGACAT CAGCCATCAA TAAGGCACAG AGTGGCAATC CAAGCTGGGG AGGCTATCCT 
AGCATCCATG CTGCCTACCA GTTCCCCAGT GCCCTTAAGC AAGGAAGCAT GGAAAAGAAT 
TCACAAATGA AGTTCTTGTT CAATGGAGGC GATGGAGCGC CGTCCTCCCT TGCCAAGAAC 
CAGCCCCTCA TTTCCCCACC ACTTAGTCAG TCGTCACTTT TTCCCAGCAA CAACTTTCAG 
GCAATGGAAG ACTTAGTGAA AAAAGTGACT GAGAAAGTAG CAAAAGTAGA ACAAAGAGTT 
AAGCAGTTAT CTCCAAAGAG GGAGAACCAG TTCTCCCCCT GCAGCAGTGA GGCTGGAGAA 
TCACACAAGG GAGGGGAGGC AGACTCGCCT CGGGAATGGA GGGAAGTTAC CCCAACCAAT 
AGCGACAGGG GAAGCCATGG TGACAGAGCA TCTCCAGCAA CCGAACCCAA GAGAGAAGCA 
GCAGTGAAAT CCCCGCTTGC CTCTACACTG AGATGCAGCA CCGCCATTAT CACTGGTCAC 
ACTCCTCCAG AGCAGCCCTT TGTCAACCCT CTAAGTGCTC TTCAGTCAGT AATGAACATT 
CACTTGGGGA AAGCAGCCAA GCCCTCTTTG CCAAACCAAG ATCCCTTGAG TCTCCTCTCT 
AGGCTCAGCC AGAGCATGGC TGAGAAGGCC GCTGTGGCTG CTCCTCCATC ACAGACCAAA 
AAGCAAGAAA GTGTAGCTAA TAACAATAGC TTTTGCCAGC CAAATGATGA CCAACCCATG 
GACCTTACAA AAGGGAAAAG TGATCGAGGG GACTCTGTAA ACTCAGCTCC TCTAACGCCA 
TCATCCACAG CCTCCTCAGT CTCCCCCATC TCTCTTGTTA CTCCTGCAAA GCTAACAGTG 
GTCTCTCCAT ACACATCCAG CAGCCCTCTA CACGAAAATG CATTGTCGGA TATCTCAGAC 
ATGCTGAGAA ACCTTACACA GTCCCACCAT GTCACAAAGT CGGCGTCACG CCCACGTGTG 
ACGGATAAAA CTGAAGTTGT GGGCTCTACC CATGACGATG ATGACGTGTC CCTGCATGGC 
CACAAACGAA AGGGTCGTCA CTCCAACTGG AACCCACAGC ACCTCCTTCT TCTGCAGGCC 
CAGTTTGCCT CAAGCTTGAG GCAGACGTCA GAGGGGAAAT ATATCATTAA TGACCTCAGC 
CCACAAGAAA GAATGCACAT ATCTCGTTTC ACGGGTCTCT CCATGACCAC CATCAGCCAC 
TGGCTGGCCA ACGTGAAGTA CCAGCTACGG AGAACGGGCA GAACGAAGTT CCTCAAGAAC 
CTAGATTCCG GTCAACCTAT ATTCTTCTGC AGTGACTGTG CCTCGCAGAT TCGAACCCCA 
GCAGCTTACG TATGCCACCT GGAAGCCCAC TTGGGGTTCA GAATCAGGGA CCTGGCCAAG 
CTGTCTCCCA AACAGACTGT CAGGGACTCT CAGACTCTCA CTGAGAAAGT AGTGCCCCTG 
GAGTCCTTTC TTCCTCCACA GTCGCAAGAT GACTGCAGTA GTAATGGGGG AGTTTATCGC 
TGCCAGCTCT GTGTCCGTAA GTTTGCCACT AAGCATGCCA TCAAGCTTCA CCTCAGCAAG 
AGCCATGGGA AGTCTCCAGA GGACCATCTG CTATACGTGT GTGAGACGGA GAAATGTTAA