tnk2 gene cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

mRNA	Protein	Name
XM_005458090.3	XP_005458147.1	activated CDC42 kinase 1 isoform X3
XM_019347434.1	XP_019202979.1	activated CDC42 kinase 1 isoform X1
XM_019347436.1	XP_019202981.1	activated CDC42 kinase 1 isoform X4
XM_005458091.3	XP_005458148.1	activated CDC42 kinase 1 isoform X5
XM_025900623.1	XP_025756408.1	activated CDC42 kinase 1 isoform X2
XM_005458091.4	XP_005458148.1	activated CDC42 kinase 1 isoform X3
XM_005458090.4	XP_005458147.1	activated CDC42 kinase 1 isoform X1
XM_025900626.1	XP_025756411.1	activated CDC42 kinase 1 isoform X5
XM_025900624.1	XP_025756409.1	activated CDC42 kinase 1 isoform X4
XM_025900625.1	XP_025756410.1	activated CDC42 kinase 1 isoform X5
XM_019347435.1	XP_019202980.1	activated CDC42 kinase 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr178725	XM_025900623.1 Latest version!	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr178726	XM_025900624.1 Latest version!	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr178727	XM_025900625.1 Latest version!	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr178727	XM_025900626.1 Latest version!	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOr45856	XM_019347434.1 Latest version!	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr45857	XM_019347435.1 Latest version!	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr45858	XM_005458090.4 Latest version!	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr45859	XM_019347436.1 Latest version!	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr45860	XM_005458091.4 Latest version!	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OOr45860	XM_005458091.3	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOr45858	XM_005458090.3	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

tnk2 ( XM_025900623.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_025756408.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOr178725
Clone ID Related Accession (Same CDS sequence)	XM_025900623.1
Accession Version	XM_025900623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCT TTGACAAGCT GAAAAAGACA TTCCCCTTCC TGGCACGCTT TCACGTATAT 
CGAAAACTGG GCAGCAGCAT GCAATGTGAG GAAGGGACGG AATGGTTGCT GGAACTGCTG 
ATGGAAGTGC AGCTGCAGCA GTACTTCTTA CGGATCCGAG ATGACCTTAA TGTCACGCGG 
CTGTCGCACT TTGACTACGT CAAGAATGAA GACCTGGAGA AAATCGGCAT GGGTCGACCT 
GGGCAGAGAA GGCTGTGGGA GGCCGTGAAA AGGAGGAAAG CCATGTGCAA GCGCAAGTCC 
TGGATGAGCA AGGTTTTTAG TGGTAAACGT CCAGATGGAG GAGACGTGCC CCAGCAGGGT 
CAACCGGCTT CCTCGTTTCG TAAGCTGTCT CCCACACCAC CTCTGGGCGT CGGGGAGGGA 
GTCCTAGTCA CGCAGCCCAG CGGTGGCGCT CTCCTCGATG GGCAGCAGCA GGGTCTGACC 
TGCCTCATCC CAGAGAAGGA CTTGACGCTG TTTGAGAAGC TGGGTGACGG CTCATTTGGG 
GTCGTGAAGA GGGGAGAGTG GTTGACACCT GCGGGGAAGG TGCTGAACGT GGCCGTGAAG 
TGTCTGAAGA CAGATGTGCT CAGCCAGCCC GACGCTCTGG AGGATTTCAT CTGCGAGGTC 
AACGTCATGC ACTCGCTGGA CCATCAAAAC CTGATCCGCC TCTACGGTGT GGTGCTCACA 
CACCCAATGA AGATGGTGAC TGAACTGGCA CCTCTCGGTT CTCTGCTGGA GCGTTTGCGC 
TGCGTTCGTC CTCAAGGCCC AGTGTTGATC CACACTCTGT GTCAGTATGC TGTGCAGGTG 
GCTTGTGGCA TGGCTTATCT GGAGCAGAGG AGGTTCATCC ACAGGGACCT GGCAGCGAGG 
AACATCCTGC TGGCCGCGGC TCACAGAGTG AAGATTGGTG ACTTTGGCCT GATGAGGGCG 
CTGCCTAACA ACCACGAGCA CTATGTCATG CAGGAGCATC GCAAGGTCCC CTTTGCATGG 
TGTGCCCCAG AGAGTTTAAA GACCAGAACC TTCTCCCATG CAACAGACAC TTGGATGTTT 
GGAGTCACCC TCTGGGAGAT GTTCACACAT GGTCAGGAGC CTTGGCTGGG TCTTAATGGT 
AGTCAGATTC TCCATAAGAT TGATAAAGAG GGTGAACGCC TCCCTAAGCC AGAGGATTGT 
CCACAGGATA TCTATAACGT CATGCTGCAG TGCTGGGCTC AGAAACCTGA TGACAGACCA 
ACTTTTGTTG CTCTACGCGA GTTCCTGCTC GAGTCTATGC CTACAGACAT GTGTGCTCTC 
CAGGACTTTG ATGAGCCTGA CAAACTCCAG ATCCAGGTCG ATGACATTAT CACCATCATA 
GAGGGGAGGG CAGAGAATTA TTGGTGGCGA GGTCAAAATA AGCGCACCCT TAAGGTCGGG 
CAGTTCCCCA GGAATGTGGT GACTTCTGTA GCAGGGTTGT CTGCACACGA CATCAGCCGT 
CCACTCAAGA ACAGCTTCAT TCACACAGGA CATGGAGACA CCAACCCCCA TCGCTGCTGG 
GGCTTCCCTG ACAGGATCGA TGATTTGTAC CTCGGGAATC CCATGGATCC TCCTGACGTT 
CTGGGCTTGG ACCTCAGTGC TGCACAGCCC ACACAGCTAG CAGGACGAGC TAAAAAGGAG 
CCTCCTCCCC GCCCTCCTCA ACCAGCAGTG TTAATCAAGA AACCTTCCTA TGACCCAGTA 
AATGAGGAGG AGGATCTGAC TTCTGCGGGA CTAAAGAGAT TATCACTTCG GAAAACGGGT 
TCTGTCAAAG GCCTCAAACT AAAACCGGCC GTGTGGGTCT CTGCTTCCAA ACAGGGAGGT 
GGCAGGACTT CAGGCTCAGG CCAGAACACT AACAGTGAAG TGTCCCTCAT TGACTTTGGG 
GAAGAGTACC CCATATCCAC ACCGTCCCCC TCTCCCGTGG TTGAAATTCC AATTCCTCTA 
TTGGCCAAGT TGGCTTTGGA AGCAGAAAAT CTCCTGGACA GGACTCCACC TCAAAGCCCA 
TCCAAATCAT TGCCCCGCCC CCTTCACCCT ACGCCAGTAG TGGACTGGGA TGCGCGGCCA 
TTACCCCCAC CCCCAGCTTA TGACGATGTA GCGCAAGATG AGGATGATAT GGAGGTGAGC 
TCCATCAACA GCTCGGAGCA GCAGAATGAG GCAGAGCATA GTGATGGCCT TAATCCAGAA 
GAAACTCTGT CCTCTGGGCA AAAGGTCAAT GATGAGGCTC TAGTCTCCAG AGGTTCAGAC 
AGACCAGGTC TGGAAGACAA CCTGTTTCTC CCCAGCAACC ACAGCCAGGG CCTGTCCACC 
TCTTTTTCAC AGTCTGCAGA GATCTTTCAA GAACTACAAC AAGAGTGCAT GAGGAGGCTC 
AATGTACCAG CAGCAAGTGC TCCTCGTTCG TGCTCGCCAT TGCAGAGCTC AGTCTTGTGT 
CCCCAGACTC TTCAAAGTCC CCAAACCCAT GAGGGACAAC AGCAGAGTGT CCTCTCCTGT 
AACGAGGACC GACCCCAGAT CCCCCCACGA GTTCCCATCC CCCCTCGCCC CATGAAGAAG 
GGGGACTACA CCTCTGGCCG CTGGTCAAGG GACCTCTCCC TTTCTCCAGT GCCGACTGAA 
ACCACGGAAA ACGTTTCAGG CGCAGGCCAG GAACGACCAC CTCAAATCCC TCCCAGAGAC 
CCTTTGTCTC AGCCTGGATC AAGAACTCCA AGCCCAATGG GTCTGGTAGT GGGTTCCCCC 
CAGCAGAGGC TCTACTCTGT CAGCCCCAAC ACAATGCAGG CTGCCATTAC AACCTGCCCA 
CCAACACACA CCTACAGCTC CTACCTCTCT ACCTCTCCAG GTAAACTGAT GCCTACTACA 
CACAGCTTTG CTTCGGACCC TAAATATGCT GCACCCAAAG TGATCCAGGC CCAGGTGCAG 
GGGAAGGACG CTGCTAGCAA GGGCCCCTGT ATCCTGCCCA TCGTCCGTGA CGGGCGTAAA 
GTCAGTAACA CCCACTATTA CCTTCTGCCA GAGAGGCCAC CGTATCTCGA TCGATTCGAT 
CGCTTCTTCA GGGAGGCTGA AAGCCTTCCC GCCAACGATG TGGAAGAAAA GCATGTACGG 
CAAGCCAACA CAGCCACGGT CAGACCGATG GTGGTCGGCA GTCACACACT GCAGGGGCAC 
ACCCAGGTGC AGGTCCAGCC GGGTGGTGAC CTGAAAGCCA ATTTCTCCTC CAATAATAAC 
AGCAATCTGG GTTGGCCACG GTCAGGGATG AAGAGTTCAG TTAGTCTTCC TCGCGTCTGT 
TCAGACGGGC TGACAGCACC AGCAGTCACT GCTTTCTGTA CCAGGACAGA CGGAGGAGCG 
AACTCAGCTG ATAGGGTCAA GATGGTGCAG GAGGCGGTTC ACGGTGTGAC GATGGAGGAG 
TGCCAAGCCG CACTCCACAA CCACAACTGG AATGTCCAGC AAGCTGTGCA TTATCTAAAG 
GTAGAACAGT TGTTCTGTTT GGGCCTGAGG AGCAGATCAG AGTGTCTAAA GCTGCTGGAG 
ATGTGTGACT GGAACCTGGA GGTGGCCAGC ACTCACATGC TGGATAACTA TGGATCTACA 
ACACGACAAA GACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025756408.1
CDS	468..4085
Translation	MRRFDKLKKTFPFLARFHVYRKLGSSMQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKEPPPRPPQPAVLIKKPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_025900623.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025900623.1

ORF Insert Sequence:

ATGCGACGCT TTGACAAGCT GAAAAAGACA TTCCCCTTCC TGGCACGCTT TCACGTATAT 
CGAAAACTGG GCAGCAGCAT GCAATGTGAG GAAGGGACGG AATGGTTGCT GGAACTGCTG 
ATGGAAGTGC AGCTGCAGCA GTACTTCTTA CGGATCCGAG ATGACCTTAA TGTCACGCGG 
CTGTCGCACT TTGACTACGT CAAGAATGAA GACCTGGAGA AAATCGGCAT GGGTCGACCT 
GGGCAGAGAA GGCTGTGGGA GGCCGTGAAA AGGAGGAAAG CCATGTGCAA GCGCAAGTCC 
TGGATGAGCA AGGTTTTTAG TGGTAAACGT CCAGATGGAG GAGACGTGCC CCAGCAGGGT 
CAACCGGCTT CCTCGTTTCG TAAGCTGTCT CCCACACCAC CTCTGGGCGT CGGGGAGGGA 
GTCCTAGTCA CGCAGCCCAG CGGTGGCGCT CTCCTCGATG GGCAGCAGCA GGGTCTGACC 
TGCCTCATCC CAGAGAAGGA CTTGACGCTG TTTGAGAAGC TGGGTGACGG CTCATTTGGG 
GTCGTGAAGA GGGGAGAGTG GTTGACACCT GCGGGGAAGG TGCTGAACGT GGCCGTGAAG 
TGTCTGAAGA CAGATGTGCT CAGCCAGCCC GACGCTCTGG AGGATTTCAT CTGCGAGGTC 
AACGTCATGC ACTCGCTGGA CCATCAAAAC CTGATCCGCC TCTACGGTGT GGTGCTCACA 
CACCCAATGA AGATGGTGAC TGAACTGGCA CCTCTCGGTT CTCTGCTGGA GCGTTTGCGC 
TGCGTTCGTC CTCAAGGCCC AGTGTTGATC CACACTCTGT GTCAGTATGC TGTGCAGGTG 
GCTTGTGGCA TGGCTTATCT GGAGCAGAGG AGGTTCATCC ACAGGGACCT GGCAGCGAGG 
AACATCCTGC TGGCCGCGGC TCACAGAGTG AAGATTGGTG ACTTTGGCCT GATGAGGGCG 
CTGCCTAACA ACCACGAGCA CTATGTCATG CAGGAGCATC GCAAGGTCCC CTTTGCATGG 
TGTGCCCCAG AGAGTTTAAA GACCAGAACC TTCTCCCATG CAACAGACAC TTGGATGTTT 
GGAGTCACCC TCTGGGAGAT GTTCACACAT GGTCAGGAGC CTTGGCTGGG TCTTAATGGT 
AGTCAGATTC TCCATAAGAT TGATAAAGAG GGTGAACGCC TCCCTAAGCC AGAGGATTGT 
CCACAGGATA TCTATAACGT CATGCTGCAG TGCTGGGCTC AGAAACCTGA TGACAGACCA 
ACTTTTGTTG CTCTACGCGA GTTCCTGCTC GAGTCTATGC CTACAGACAT GTGTGCTCTC 
CAGGACTTTG ATGAGCCTGA CAAACTCCAG ATCCAGGTCG ATGACATTAT CACCATCATA 
GAGGGGAGGG CAGAGAATTA TTGGTGGCGA GGTCAAAATA AGCGCACCCT TAAGGTCGGG 
CAGTTCCCCA GGAATGTGGT GACTTCTGTA GCAGGGTTGT CTGCACACGA CATCAGCCGT 
CCACTCAAGA ACAGCTTCAT TCACACAGGA CATGGAGACA CCAACCCCCA TCGCTGCTGG 
GGCTTCCCTG ACAGGATCGA TGATTTGTAC CTCGGGAATC CCATGGATCC TCCTGACGTT 
CTGGGCTTGG ACCTCAGTGC TGCACAGCCC ACACAGCTAG CAGGACGAGC TAAAAAGGAG 
CCTCCTCCCC GCCCTCCTCA ACCAGCAGTG TTAATCAAGA AACCTTCCTA TGACCCAGTA 
AATGAGGAGG AGGATCTGAC TTCTGCGGGA CTAAAGAGAT TATCACTTCG GAAAACGGGT 
TCTGTCAAAG GCCTCAAACT AAAACCGGCC GTGTGGGTCT CTGCTTCCAA ACAGGGAGGT 
GGCAGGACTT CAGGCTCAGG CCAGAACACT AACAGTGAAG TGTCCCTCAT TGACTTTGGG 
GAAGAGTACC CCATATCCAC ACCGTCCCCC TCTCCCGTGG TTGAAATTCC AATTCCTCTA 
TTGGCCAAGT TGGCTTTGGA AGCAGAAAAT CTCCTGGACA GGACTCCACC TCAAAGCCCA 
TCCAAATCAT TGCCCCGCCC CCTTCACCCT ACGCCAGTAG TGGACTGGGA TGCGCGGCCA 
TTACCCCCAC CCCCAGCTTA TGACGATGTA GCGCAAGATG AGGATGATAT GGAGGTGAGC 
TCCATCAACA GCTCGGAGCA GCAGAATGAG GCAGAGCATA GTGATGGCCT TAATCCAGAA 
GAAACTCTGT CCTCTGGGCA AAAGGTCAAT GATGAGGCTC TAGTCTCCAG AGGTTCAGAC 
AGACCAGGTC TGGAAGACAA CCTGTTTCTC CCCAGCAACC ACAGCCAGGG CCTGTCCACC 
TCTTTTTCAC AGTCTGCAGA GATCTTTCAA GAACTACAAC AAGAGTGCAT GAGGAGGCTC 
AATGTACCAG CAGCAAGTGC TCCTCGTTCG TGCTCGCCAT TGCAGAGCTC AGTCTTGTGT 
CCCCAGACTC TTCAAAGTCC CCAAACCCAT GAGGGACAAC AGCAGAGTGT CCTCTCCTGT 
AACGAGGACC GACCCCAGAT CCCCCCACGA GTTCCCATCC CCCCTCGCCC CATGAAGAAG 
GGGGACTACA CCTCTGGCCG CTGGTCAAGG GACCTCTCCC TTTCTCCAGT GCCGACTGAA 
ACCACGGAAA ACGTTTCAGG CGCAGGCCAG GAACGACCAC CTCAAATCCC TCCCAGAGAC 
CCTTTGTCTC AGCCTGGATC AAGAACTCCA AGCCCAATGG GTCTGGTAGT GGGTTCCCCC 
CAGCAGAGGC TCTACTCTGT CAGCCCCAAC ACAATGCAGG CTGCCATTAC AACCTGCCCA 
CCAACACACA CCTACAGCTC CTACCTCTCT ACCTCTCCAG GTAAACTGAT GCCTACTACA 
CACAGCTTTG CTTCGGACCC TAAATATGCT GCACCCAAAG TGATCCAGGC CCAGGTGCAG 
GGGAAGGACG CTGCTAGCAA GGGCCCCTGT ATCCTGCCCA TCGTCCGTGA CGGGCGTAAA 
GTCAGTAACA CCCACTATTA CCTTCTGCCA GAGAGGCCAC CGTATCTCGA TCGATTCGAT 
CGCTTCTTCA GGGAGGCTGA AAGCCTTCCC GCCAACGATG TGGAAGAAAA GCATGTACGG 
CAAGCCAACA CAGCCACGGT CAGACCGATG GTGGTCGGCA GTCACACACT GCAGGGGCAC 
ACCCAGGTGC AGGTCCAGCC GGGTGGTGAC CTGAAAGCCA ATTTCTCCTC CAATAATAAC 
AGCAATCTGG GTTGGCCACG GTCAGGGATG AAGAGTTCAG TTAGTCTTCC TCGCGTCTGT 
TCAGACGGGC TGACAGCACC AGCAGTCACT GCTTTCTGTA CCAGGACAGA CGGAGGAGCG 
AACTCAGCTG ATAGGGTCAA GATGGTGCAG GAGGCGGTTC ACGGTGTGAC GATGGAGGAG 
TGCCAAGCCG CACTCCACAA CCACAACTGG AATGTCCAGC AAGCTGTGCA TTATCTAAAG 
GTAGAACAGT TGTTCTGTTT GGGCCTGAGG AGCAGATCAG AGTGTCTAAA GCTGCTGGAG 
ATGTGTGACT GGAACCTGGA GGTGGCCAGC ACTCACATGC TGGATAACTA TGGATCTACA 
ACACGACAAA GACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_025900624.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_025756409.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOr178726
Clone ID Related Accession (Same CDS sequence)	XM_025900624.1
Accession Version	XM_025900624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCT TTGACAAGCT GAAAAAGACA TTCCCCTTCC TGGCACGCTT TCACGTATAT 
CGAAAACTGG GCAGCAGCAT GCAATGTGAG GAAGGGACGG AATGGTTGCT GGAACTGCTG 
ATGGAAGTGC AGCTGCAGCA GTACTTCTTA CGGATCCGAG ATGACCTTAA TGTCACGCGG 
CTGTCGCACT TTGACTACGT CAAGAATGAA GACCTGGAGA AAATCGGCAT GGGTCGACCT 
GGGCAGAGAA GGCTGTGGGA GGCCGTGAAA AGGAGGAAAG CCATGTGCAA GCGCAAGTCC 
TGGATGAGCA AGGTTTTTAG TGGTAAACGT CCAGATGGAG GAGACGTGCC CCAGCAGGGT 
CAACCGGCTT CCTCGTTTCG TAAGCTGTCT CCCACACCAC CTCTGGGCGT CGGGGAGGGA 
GTCCTAGTCA CGCAGCCCAG CGGTGGCGCT CTCCTCGATG GGCAGCAGCA GGGTCTGACC 
TGCCTCATCC CAGAGAAGGA CTTGACGCTG TTTGAGAAGC TGGGTGACGG CTCATTTGGG 
GTCGTGAAGA GGGGAGAGTG GTTGACACCT GCGGGGAAGG TGCTGAACGT GGCCGTGAAG 
TGTCTGAAGA CAGATGTGCT CAGCCAGCCC GACGCTCTGG AGGATTTCAT CTGCGAGGTC 
AACGTCATGC ACTCGCTGGA CCATCAAAAC CTGATCCGCC TCTACGGTGT GGTGCTCACA 
CACCCAATGA AGATGGTGAC TGAACTGGCA CCTCTCGGTT CTCTGCTGGA GCGTTTGCGC 
TGCGTTCGTC CTCAAGGCCC AGTGTTGATC CACACTCTGT GTCAGTATGC TGTGCAGGTG 
GCTTGTGGCA TGGCTTATCT GGAGCAGAGG AGGTTCATCC ACAGGGACCT GGCAGCGAGG 
AACATCCTGC TGGCCGCGGC TCACAGAGTG AAGATTGGTG ACTTTGGCCT GATGAGGGCG 
CTGCCTAACA ACCACGAGCA CTATGTCATG CAGGAGCATC GCAAGGTCCC CTTTGCATGG 
TGTGCCCCAG AGAGTTTAAA GACCAGAACC TTCTCCCATG CAACAGACAC TTGGATGTTT 
GGAGTCACCC TCTGGGAGAT GTTCACACAT GGTCAGGAGC CTTGGCTGGG TCTTAATGGT 
AGTCAGATTC TCCATAAGAT TGATAAAGAG GGTGAACGCC TCCCTAAGCC AGAGGATTGT 
CCACAGGATA TCTATAACGT CATGCTGCAG TGCTGGGCTC AGAAACCTGA TGACAGACCA 
ACTTTTGTTG CTCTACGCGA GTTCCTGCTC GAGTCTATGC CTACAGACAT GTGTGCTCTC 
CAGGACTTTG ATGAGCCTGA CAAACTCCAG ATCCAGGTCG ATGACATTAT CACCATCATA 
GAGGGGAGGG CAGAGAATTA TTGGTGGCGA GGTCAAAATA AGCGCACCCT TAAGGTCGGG 
CAGTTCCCCA GGAATGTGGT GACTTCTGTA GCAGGGTTGT CTGCACACGA CATCAGCCGT 
CCACTCAAGA ACAGCTTCAT TCACACAGGA CATGGAGACA CCAACCCCCA TCGCTGCTGG 
GGCTTCCCTG ACAGGATCGA TGATTTGTAC CTCGGGAATC CCATGGATCC TCCTGACGTT 
CTGGGCTTGG ACCTCAGTGC TGCACAGCCC ACACAGCTAG CAGGACGAGC TAAAAAACCT 
TCCTATGACC CAGTAAATGA GGAGGAGGAT CTGACTTCTG CGGGACTAAA GAGATTATCA 
CTTCGGAAAA CGGGTTCTGT CAAAGGCCTC AAACTAAAAC CGGCCGTGTG GGTCTCTGCT 
TCCAAACAGG GAGGTGGCAG GACTTCAGGC TCAGGCCAGA ACACTAACAG TGAAGTGTCC 
CTCATTGACT TTGGGGAAGA GTACCCCATA TCCACACCGT CCCCCTCTCC CGTGGTTGAA 
ATTCCAATTC CTCTATTGGC CAAGTTGGCT TTGGAAGCAG AAAATCTCCT GGACAGGACT 
CCACCTCAAA GCCCATCCAA ATCATTGCCC CGCCCCCTTC ACCCTACGCC AGTAGTGGAC 
TGGGATGCGC GGCCATTACC CCCACCCCCA GCTTATGACG ATGTAGCGCA AGATGAGGAT 
GATATGGAGG TGAGCTCCAT CAACAGCTCG GAGCAGCAGA ATGAGGCAGA GCATAGTGAT 
GGCCTTAATC CAGAAGAAAC TCTGTCCTCT GGGCAAAAGG TCAATGATGA GGCTCTAGTC 
TCCAGAGGTT CAGACAGACC AGGTCTGGAA GACAACCTGT TTCTCCCCAG CAACCACAGC 
CAGGGCCTGT CCACCTCTTT TTCACAGTCT GCAGAGATCT TTCAAGAACT ACAACAAGAG 
TGCATGAGGA GGCTCAATGT ACCAGCAGCA AGTGCTCCTC GTTCGTGCTC GCCATTGCAG 
AGCTCAGTCT TGTGTCCCCA GACTCTTCAA AGTCCCCAAA CCCATGAGGG ACAACAGCAG 
AGTGTCCTCT CCTGTAACGA GGACCGACCC CAGATCCCCC CACGAGTTCC CATCCCCCCT 
CGCCCCATGA AGAAGGGGGA CTACACCTCT GGCCGCTGGT CAAGGGACCT CTCCCTTTCT 
CCAGTGCCGA CTGAAACCAC GGAAAACGTT TCAGGCGCAG GCCAGGAACG ACCACCTCAA 
ATCCCTCCCA GAGACCCTTT GTCTCAGCCT GGATCAAGAA CTCCAAGCCC AATGGGTCTG 
GTAGTGGGTT CCCCCCAGCA GAGGCTCTAC TCTGTCAGCC CCAACACAAT GCAGGCTGCC 
ATTACAACCT GCCCACCAAC ACACACCTAC AGCTCCTACC TCTCTACCTC TCCAGGTAAA 
CTGATGCCTA CTACACACAG CTTTGCTTCG GACCCTAAAT ATGCTGCACC CAAAGTGATC 
CAGGCCCAGG TGCAGGGGAA GGACGCTGCT AGCAAGGGCC CCTGTATCCT GCCCATCGTC 
CGTGACGGGC GTAAAGTCAG TAACACCCAC TATTACCTTC TGCCAGAGAG GCCACCGTAT 
CTCGATCGAT TCGATCGCTT CTTCAGGGAG GCTGAAAGCC TTCCCGCCAA CGATGTGGAA 
GAAAAGCATG TACGGCAAGC CAACACAGCC ACGGTCAGAC CGATGGTGGT CGGCAGTCAC 
ACACTGCAGG GGCACACCCA GGTGCAGGTC CAGCCGGGTG GTGACCTGAA AGCCAATTTC 
TCCTCCAATA ATAACAGCAA TCTGGGTTGG CCACGGTCAG GGATGAAGAG TTCAGTTAGT 
CTTCCTCGCG TCTGTTCAGA CGGGCTGACA GCACCAGCAG TCACTGCTTT CTGTACCAGG 
ACAGACGGAG GAGCGAACTC AGCTGATAGG GTCAAGATGG TGCAGGAGGC GGTTCACGGT 
GTGACGATGG AGGAGTGCCA AGCCGCACTC CACAACCACA ACTGGAATGT CCAGCAAGCT 
GTGCATTATC TAAAGGTAGA ACAGTTGTTC TGTTTGGGCC TGAGGAGCAG ATCAGAGTGT 
CTAAAGCTGC TGGAGATGTG TGACTGGAAC CTGGAGGTGG CCAGCACTCA CATGCTGGAT 
AACTATGGAT CTACAACACG ACAAAGACGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025756409.1
CDS	467..4039
Translation	MRRFDKLKKTFPFLARFHVYRKLGSSMQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_025900624.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025900624.1

ORF Insert Sequence:

ATGCGACGCT TTGACAAGCT GAAAAAGACA TTCCCCTTCC TGGCACGCTT TCACGTATAT 
CGAAAACTGG GCAGCAGCAT GCAATGTGAG GAAGGGACGG AATGGTTGCT GGAACTGCTG 
ATGGAAGTGC AGCTGCAGCA GTACTTCTTA CGGATCCGAG ATGACCTTAA TGTCACGCGG 
CTGTCGCACT TTGACTACGT CAAGAATGAA GACCTGGAGA AAATCGGCAT GGGTCGACCT 
GGGCAGAGAA GGCTGTGGGA GGCCGTGAAA AGGAGGAAAG CCATGTGCAA GCGCAAGTCC 
TGGATGAGCA AGGTTTTTAG TGGTAAACGT CCAGATGGAG GAGACGTGCC CCAGCAGGGT 
CAACCGGCTT CCTCGTTTCG TAAGCTGTCT CCCACACCAC CTCTGGGCGT CGGGGAGGGA 
GTCCTAGTCA CGCAGCCCAG CGGTGGCGCT CTCCTCGATG GGCAGCAGCA GGGTCTGACC 
TGCCTCATCC CAGAGAAGGA CTTGACGCTG TTTGAGAAGC TGGGTGACGG CTCATTTGGG 
GTCGTGAAGA GGGGAGAGTG GTTGACACCT GCGGGGAAGG TGCTGAACGT GGCCGTGAAG 
TGTCTGAAGA CAGATGTGCT CAGCCAGCCC GACGCTCTGG AGGATTTCAT CTGCGAGGTC 
AACGTCATGC ACTCGCTGGA CCATCAAAAC CTGATCCGCC TCTACGGTGT GGTGCTCACA 
CACCCAATGA AGATGGTGAC TGAACTGGCA CCTCTCGGTT CTCTGCTGGA GCGTTTGCGC 
TGCGTTCGTC CTCAAGGCCC AGTGTTGATC CACACTCTGT GTCAGTATGC TGTGCAGGTG 
GCTTGTGGCA TGGCTTATCT GGAGCAGAGG AGGTTCATCC ACAGGGACCT GGCAGCGAGG 
AACATCCTGC TGGCCGCGGC TCACAGAGTG AAGATTGGTG ACTTTGGCCT GATGAGGGCG 
CTGCCTAACA ACCACGAGCA CTATGTCATG CAGGAGCATC GCAAGGTCCC CTTTGCATGG 
TGTGCCCCAG AGAGTTTAAA GACCAGAACC TTCTCCCATG CAACAGACAC TTGGATGTTT 
GGAGTCACCC TCTGGGAGAT GTTCACACAT GGTCAGGAGC CTTGGCTGGG TCTTAATGGT 
AGTCAGATTC TCCATAAGAT TGATAAAGAG GGTGAACGCC TCCCTAAGCC AGAGGATTGT 
CCACAGGATA TCTATAACGT CATGCTGCAG TGCTGGGCTC AGAAACCTGA TGACAGACCA 
ACTTTTGTTG CTCTACGCGA GTTCCTGCTC GAGTCTATGC CTACAGACAT GTGTGCTCTC 
CAGGACTTTG ATGAGCCTGA CAAACTCCAG ATCCAGGTCG ATGACATTAT CACCATCATA 
GAGGGGAGGG CAGAGAATTA TTGGTGGCGA GGTCAAAATA AGCGCACCCT TAAGGTCGGG 
CAGTTCCCCA GGAATGTGGT GACTTCTGTA GCAGGGTTGT CTGCACACGA CATCAGCCGT 
CCACTCAAGA ACAGCTTCAT TCACACAGGA CATGGAGACA CCAACCCCCA TCGCTGCTGG 
GGCTTCCCTG ACAGGATCGA TGATTTGTAC CTCGGGAATC CCATGGATCC TCCTGACGTT 
CTGGGCTTGG ACCTCAGTGC TGCACAGCCC ACACAGCTAG CAGGACGAGC TAAAAAACCT 
TCCTATGACC CAGTAAATGA GGAGGAGGAT CTGACTTCTG CGGGACTAAA GAGATTATCA 
CTTCGGAAAA CGGGTTCTGT CAAAGGCCTC AAACTAAAAC CGGCCGTGTG GGTCTCTGCT 
TCCAAACAGG GAGGTGGCAG GACTTCAGGC TCAGGCCAGA ACACTAACAG TGAAGTGTCC 
CTCATTGACT TTGGGGAAGA GTACCCCATA TCCACACCGT CCCCCTCTCC CGTGGTTGAA 
ATTCCAATTC CTCTATTGGC CAAGTTGGCT TTGGAAGCAG AAAATCTCCT GGACAGGACT 
CCACCTCAAA GCCCATCCAA ATCATTGCCC CGCCCCCTTC ACCCTACGCC AGTAGTGGAC 
TGGGATGCGC GGCCATTACC CCCACCCCCA GCTTATGACG ATGTAGCGCA AGATGAGGAT 
GATATGGAGG TGAGCTCCAT CAACAGCTCG GAGCAGCAGA ATGAGGCAGA GCATAGTGAT 
GGCCTTAATC CAGAAGAAAC TCTGTCCTCT GGGCAAAAGG TCAATGATGA GGCTCTAGTC 
TCCAGAGGTT CAGACAGACC AGGTCTGGAA GACAACCTGT TTCTCCCCAG CAACCACAGC 
CAGGGCCTGT CCACCTCTTT TTCACAGTCT GCAGAGATCT TTCAAGAACT ACAACAAGAG 
TGCATGAGGA GGCTCAATGT ACCAGCAGCA AGTGCTCCTC GTTCGTGCTC GCCATTGCAG 
AGCTCAGTCT TGTGTCCCCA GACTCTTCAA AGTCCCCAAA CCCATGAGGG ACAACAGCAG 
AGTGTCCTCT CCTGTAACGA GGACCGACCC CAGATCCCCC CACGAGTTCC CATCCCCCCT 
CGCCCCATGA AGAAGGGGGA CTACACCTCT GGCCGCTGGT CAAGGGACCT CTCCCTTTCT 
CCAGTGCCGA CTGAAACCAC GGAAAACGTT TCAGGCGCAG GCCAGGAACG ACCACCTCAA 
ATCCCTCCCA GAGACCCTTT GTCTCAGCCT GGATCAAGAA CTCCAAGCCC AATGGGTCTG 
GTAGTGGGTT CCCCCCAGCA GAGGCTCTAC TCTGTCAGCC CCAACACAAT GCAGGCTGCC 
ATTACAACCT GCCCACCAAC ACACACCTAC AGCTCCTACC TCTCTACCTC TCCAGGTAAA 
CTGATGCCTA CTACACACAG CTTTGCTTCG GACCCTAAAT ATGCTGCACC CAAAGTGATC 
CAGGCCCAGG TGCAGGGGAA GGACGCTGCT AGCAAGGGCC CCTGTATCCT GCCCATCGTC 
CGTGACGGGC GTAAAGTCAG TAACACCCAC TATTACCTTC TGCCAGAGAG GCCACCGTAT 
CTCGATCGAT TCGATCGCTT CTTCAGGGAG GCTGAAAGCC TTCCCGCCAA CGATGTGGAA 
GAAAAGCATG TACGGCAAGC CAACACAGCC ACGGTCAGAC CGATGGTGGT CGGCAGTCAC 
ACACTGCAGG GGCACACCCA GGTGCAGGTC CAGCCGGGTG GTGACCTGAA AGCCAATTTC 
TCCTCCAATA ATAACAGCAA TCTGGGTTGG CCACGGTCAG GGATGAAGAG TTCAGTTAGT 
CTTCCTCGCG TCTGTTCAGA CGGGCTGACA GCACCAGCAG TCACTGCTTT CTGTACCAGG 
ACAGACGGAG GAGCGAACTC AGCTGATAGG GTCAAGATGG TGCAGGAGGC GGTTCACGGT 
GTGACGATGG AGGAGTGCCA AGCCGCACTC CACAACCACA ACTGGAATGT CCAGCAAGCT 
GTGCATTATC TAAAGGTAGA ACAGTTGTTC TGTTTGGGCC TGAGGAGCAG ATCAGAGTGT 
CTAAAGCTGC TGGAGATGTG TGACTGGAAC CTGGAGGTGG CCAGCACTCA CATGCTGGAT 
AACTATGGAT CTACAACACG ACAAAGACGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_025900626.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_025756411.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X6, mRNA.

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CloneID	OOr178727
Clone ID Related Accession (Same CDS sequence)	XM_025900626.1 , XM_025900625.1
Accession Version	XM_025900626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATGTG AGGAAGGGAC GGAATGGTTG CTGGAACTGC TGATGGAAGT GCAGCTGCAG 
CAGTACTTCT TACGGATCCG AGATGACCTT AATGTCACGC GGCTGTCGCA CTTTGACTAC 
GTCAAGAATG AAGACCTGGA GAAAATCGGC ATGGGTCGAC CTGGGCAGAG AAGGCTGTGG 
GAGGCCGTGA AAAGGAGGAA AGCCATGTGC AAGCGCAAGT CCTGGATGAG CAAGGTTTTT 
AGTGGTAAAC GTCCAGATGG AGGAGACGTG CCCCAGCAGG GTCAACCGGC TTCCTCGTTT 
CGTAAGCTGT CTCCCACACC ACCTCTGGGC GTCGGGGAGG GAGTCCTAGT CACGCAGCCC 
AGCGGTGGCG CTCTCCTCGA TGGGCAGCAG CAGGGTCTGA CCTGCCTCAT CCCAGAGAAG 
GACTTGACGC TGTTTGAGAA GCTGGGTGAC GGCTCATTTG GGGTCGTGAA GAGGGGAGAG 
TGGTTGACAC CTGCGGGGAA GGTGCTGAAC GTGGCCGTGA AGTGTCTGAA GACAGATGTG 
CTCAGCCAGC CCGACGCTCT GGAGGATTTC ATCTGCGAGG TCAACGTCAT GCACTCGCTG 
GACCATCAAA ACCTGATCCG CCTCTACGGT GTGGTGCTCA CACACCCAAT GAAGATGGTG 
ACTGAACTGG CACCTCTCGG TTCTCTGCTG GAGCGTTTGC GCTGCGTTCG TCCTCAAGGC 
CCAGTGTTGA TCCACACTCT GTGTCAGTAT GCTGTGCAGG TGGCTTGTGG CATGGCTTAT 
CTGGAGCAGA GGAGGTTCAT CCACAGGGAC CTGGCAGCGA GGAACATCCT GCTGGCCGCG 
GCTCACAGAG TGAAGATTGG TGACTTTGGC CTGATGAGGG CGCTGCCTAA CAACCACGAG 
CACTATGTCA TGCAGGAGCA TCGCAAGGTC CCCTTTGCAT GGTGTGCCCC AGAGAGTTTA 
AAGACCAGAA CCTTCTCCCA TGCAACAGAC ACTTGGATGT TTGGAGTCAC CCTCTGGGAG 
ATGTTCACAC ATGGTCAGGA GCCTTGGCTG GGTCTTAATG GTAGTCAGAT TCTCCATAAG 
ATTGATAAAG AGGGTGAACG CCTCCCTAAG CCAGAGGATT GTCCACAGGA TATCTATAAC 
GTCATGCTGC AGTGCTGGGC TCAGAAACCT GATGACAGAC CAACTTTTGT TGCTCTACGC 
GAGTTCCTGC TCGAGTCTAT GCCTACAGAC ATGTGTGCTC TCCAGGACTT TGATGAGCCT 
GACAAACTCC AGATCCAGGT CGATGACATT ATCACCATCA TAGAGGGGAG GGCAGAGAAT 
TATTGGTGGC GAGGTCAAAA TAAGCGCACC CTTAAGGTCG GGCAGTTCCC CAGGAATGTG 
GTGACTTCTG TAGCAGGGTT GTCTGCACAC GACATCAGCC GTCCACTCAA GAACAGCTTC 
ATTCACACAG GACATGGAGA CACCAACCCC CATCGCTGCT GGGGCTTCCC TGACAGGATC 
GATGATTTGT ACCTCGGGAA TCCCATGGAT CCTCCTGACG TTCTGGGCTT GGACCTCAGT 
GCTGCACAGC CCACACAGCT AGCAGGACGA GCTAAAAAGG AGCCTCCTCC CCGCCCTCCT 
CAACCAGCAG TGTTAATCAA GAAACCTTCC TATGACCCAG TAAATGAGGA GGAGGATCTG 
ACTTCTGCGG GACTAAAGAG ATTATCACTT CGGAAAACGG GTTCTGTCAA AGGCCTCAAA 
CTAAAACCGG CCGTGTGGGT CTCTGCTTCC AAACAGGGAG GTGGCAGGAC TTCAGGCTCA 
GGCCAGAACA CTAACAGTGA AGTGTCCCTC ATTGACTTTG GGGAAGAGTA CCCCATATCC 
ACACCGTCCC CCTCTCCCGT GGTTGAAATT CCAATTCCTC TATTGGCCAA GTTGGCTTTG 
GAAGCAGAAA ATCTCCTGGA CAGGACTCCA CCTCAAAGCC CATCCAAATC ATTGCCCCGC 
CCCCTTCACC CTACGCCAGT AGTGGACTGG GATGCGCGGC CATTACCCCC ACCCCCAGCT 
TATGACGATG TAGCGCAAGA TGAGGATGAT ATGGAGGTGA GCTCCATCAA CAGCTCGGAG 
CAGCAGAATG AGGCAGAGCA TAGTGATGGC CTTAATCCAG AAGAAACTCT GTCCTCTGGG 
CAAAAGGTCA ATGATGAGGC TCTAGTCTCC AGAGGTTCAG ACAGACCAGG TCTGGAAGAC 
AACCTGTTTC TCCCCAGCAA CCACAGCCAG GGCCTGTCCA CCTCTTTTTC ACAGTCTGCA 
GAGATCTTTC AAGAACTACA ACAAGAGTGC ATGAGGAGGC TCAATGTACC AGCAGCAAGT 
GCTCCTCGTT CGTGCTCGCC ATTGCAGAGC TCAGTCTTGT GTCCCCAGAC TCTTCAAAGT 
CCCCAAACCC ATGAGGGACA ACAGCAGAGT GTCCTCTCCT GTAACGAGGA CCGACCCCAG 
ATCCCCCCAC GAGTTCCCAT CCCCCCTCGC CCCATGAAGA AGGGGGACTA CACCTCTGGC 
CGCTGGTCAA GGGACCTCTC CCTTTCTCCA GTGCCGACTG AAACCACGGA AAACGTTTCA 
GGCGCAGGCC AGGAACGACC ACCTCAAATC CCTCCCAGAG ACCCTTTGTC TCAGCCTGGA 
TCAAGAACTC CAAGCCCAAT GGGTCTGGTA GTGGGTTCCC CCCAGCAGAG GCTCTACTCT 
GTCAGCCCCA ACACAATGCA GGCTGCCATT ACAACCTGCC CACCAACACA CACCTACAGC 
TCCTACCTCT CTACCTCTCC AGGTAAACTG ATGCCTACTA CACACAGCTT TGCTTCGGAC 
CCTAAATATG CTGCACCCAA AGTGATCCAG GCCCAGGTGC AGGGGAAGGA CGCTGCTAGC 
AAGGGCCCCT GTATCCTGCC CATCGTCCGT GACGGGCGTA AAGTCAGTAA CACCCACTAT 
TACCTTCTGC CAGAGAGGCC ACCGTATCTC GATCGATTCG ATCGCTTCTT CAGGGAGGCT 
GAAAGCCTTC CCGCCAACGA TGTGGAAGAA AAGCATGTAC GGCAAGCCAA CACAGCCACG 
GTCAGACCGA TGGTGGTCGG CAGTCACACA CTGCAGGGGC ACACCCAGGT GCAGGTCCAG 
CCGGGTGGTG ACCTGAAAGC CAATTTCTCC TCCAATAATA ACAGCAATCT GGGTTGGCCA 
CGGTCAGGGA TGAAGAGTTC AGTTAGTCTT CCTCGCGTCT GTTCAGACGG GCTGACAGCA 
CCAGCAGTCA CTGCTTTCTG TACCAGGACA GACGGAGGAG CGAACTCAGC TGATAGGGTC 
AAGATGGTGC AGGAGGCGGT TCACGGTGTG ACGATGGAGG AGTGCCAAGC CGCACTCCAC 
AACCACAACT GGAATGTCCA GCAAGCTGTG CATTATCTAA AGGTAGAACA GTTGTTCTGT 
TTGGGCCTGA GGAGCAGATC AGAGTGTCTA AAGCTGCTGG AGATGTGTGA CTGGAACCTG 
GAGGTGGCCA GCACTCACAT GCTGGATAAC TATGGATCTA CAACACGACA AAGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025756411.1
CDS	388..3927
Translation	MQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKEPPPRPPQPAVLIKKPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_025900626.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025900626.1

ORF Insert Sequence:

ATGCAATGTG AGGAAGGGAC GGAATGGTTG CTGGAACTGC TGATGGAAGT GCAGCTGCAG 
CAGTACTTCT TACGGATCCG AGATGACCTT AATGTCACGC GGCTGTCGCA CTTTGACTAC 
GTCAAGAATG AAGACCTGGA GAAAATCGGC ATGGGTCGAC CTGGGCAGAG AAGGCTGTGG 
GAGGCCGTGA AAAGGAGGAA AGCCATGTGC AAGCGCAAGT CCTGGATGAG CAAGGTTTTT 
AGTGGTAAAC GTCCAGATGG AGGAGACGTG CCCCAGCAGG GTCAACCGGC TTCCTCGTTT 
CGTAAGCTGT CTCCCACACC ACCTCTGGGC GTCGGGGAGG GAGTCCTAGT CACGCAGCCC 
AGCGGTGGCG CTCTCCTCGA TGGGCAGCAG CAGGGTCTGA CCTGCCTCAT CCCAGAGAAG 
GACTTGACGC TGTTTGAGAA GCTGGGTGAC GGCTCATTTG GGGTCGTGAA GAGGGGAGAG 
TGGTTGACAC CTGCGGGGAA GGTGCTGAAC GTGGCCGTGA AGTGTCTGAA GACAGATGTG 
CTCAGCCAGC CCGACGCTCT GGAGGATTTC ATCTGCGAGG TCAACGTCAT GCACTCGCTG 
GACCATCAAA ACCTGATCCG CCTCTACGGT GTGGTGCTCA CACACCCAAT GAAGATGGTG 
ACTGAACTGG CACCTCTCGG TTCTCTGCTG GAGCGTTTGC GCTGCGTTCG TCCTCAAGGC 
CCAGTGTTGA TCCACACTCT GTGTCAGTAT GCTGTGCAGG TGGCTTGTGG CATGGCTTAT 
CTGGAGCAGA GGAGGTTCAT CCACAGGGAC CTGGCAGCGA GGAACATCCT GCTGGCCGCG 
GCTCACAGAG TGAAGATTGG TGACTTTGGC CTGATGAGGG CGCTGCCTAA CAACCACGAG 
CACTATGTCA TGCAGGAGCA TCGCAAGGTC CCCTTTGCAT GGTGTGCCCC AGAGAGTTTA 
AAGACCAGAA CCTTCTCCCA TGCAACAGAC ACTTGGATGT TTGGAGTCAC CCTCTGGGAG 
ATGTTCACAC ATGGTCAGGA GCCTTGGCTG GGTCTTAATG GTAGTCAGAT TCTCCATAAG 
ATTGATAAAG AGGGTGAACG CCTCCCTAAG CCAGAGGATT GTCCACAGGA TATCTATAAC 
GTCATGCTGC AGTGCTGGGC TCAGAAACCT GATGACAGAC CAACTTTTGT TGCTCTACGC 
GAGTTCCTGC TCGAGTCTAT GCCTACAGAC ATGTGTGCTC TCCAGGACTT TGATGAGCCT 
GACAAACTCC AGATCCAGGT CGATGACATT ATCACCATCA TAGAGGGGAG GGCAGAGAAT 
TATTGGTGGC GAGGTCAAAA TAAGCGCACC CTTAAGGTCG GGCAGTTCCC CAGGAATGTG 
GTGACTTCTG TAGCAGGGTT GTCTGCACAC GACATCAGCC GTCCACTCAA GAACAGCTTC 
ATTCACACAG GACATGGAGA CACCAACCCC CATCGCTGCT GGGGCTTCCC TGACAGGATC 
GATGATTTGT ACCTCGGGAA TCCCATGGAT CCTCCTGACG TTCTGGGCTT GGACCTCAGT 
GCTGCACAGC CCACACAGCT AGCAGGACGA GCTAAAAAGG AGCCTCCTCC CCGCCCTCCT 
CAACCAGCAG TGTTAATCAA GAAACCTTCC TATGACCCAG TAAATGAGGA GGAGGATCTG 
ACTTCTGCGG GACTAAAGAG ATTATCACTT CGGAAAACGG GTTCTGTCAA AGGCCTCAAA 
CTAAAACCGG CCGTGTGGGT CTCTGCTTCC AAACAGGGAG GTGGCAGGAC TTCAGGCTCA 
GGCCAGAACA CTAACAGTGA AGTGTCCCTC ATTGACTTTG GGGAAGAGTA CCCCATATCC 
ACACCGTCCC CCTCTCCCGT GGTTGAAATT CCAATTCCTC TATTGGCCAA GTTGGCTTTG 
GAAGCAGAAA ATCTCCTGGA CAGGACTCCA CCTCAAAGCC CATCCAAATC ATTGCCCCGC 
CCCCTTCACC CTACGCCAGT AGTGGACTGG GATGCGCGGC CATTACCCCC ACCCCCAGCT 
TATGACGATG TAGCGCAAGA TGAGGATGAT ATGGAGGTGA GCTCCATCAA CAGCTCGGAG 
CAGCAGAATG AGGCAGAGCA TAGTGATGGC CTTAATCCAG AAGAAACTCT GTCCTCTGGG 
CAAAAGGTCA ATGATGAGGC TCTAGTCTCC AGAGGTTCAG ACAGACCAGG TCTGGAAGAC 
AACCTGTTTC TCCCCAGCAA CCACAGCCAG GGCCTGTCCA CCTCTTTTTC ACAGTCTGCA 
GAGATCTTTC AAGAACTACA ACAAGAGTGC ATGAGGAGGC TCAATGTACC AGCAGCAAGT 
GCTCCTCGTT CGTGCTCGCC ATTGCAGAGC TCAGTCTTGT GTCCCCAGAC TCTTCAAAGT 
CCCCAAACCC ATGAGGGACA ACAGCAGAGT GTCCTCTCCT GTAACGAGGA CCGACCCCAG 
ATCCCCCCAC GAGTTCCCAT CCCCCCTCGC CCCATGAAGA AGGGGGACTA CACCTCTGGC 
CGCTGGTCAA GGGACCTCTC CCTTTCTCCA GTGCCGACTG AAACCACGGA AAACGTTTCA 
GGCGCAGGCC AGGAACGACC ACCTCAAATC CCTCCCAGAG ACCCTTTGTC TCAGCCTGGA 
TCAAGAACTC CAAGCCCAAT GGGTCTGGTA GTGGGTTCCC CCCAGCAGAG GCTCTACTCT 
GTCAGCCCCA ACACAATGCA GGCTGCCATT ACAACCTGCC CACCAACACA CACCTACAGC 
TCCTACCTCT CTACCTCTCC AGGTAAACTG ATGCCTACTA CACACAGCTT TGCTTCGGAC 
CCTAAATATG CTGCACCCAA AGTGATCCAG GCCCAGGTGC AGGGGAAGGA CGCTGCTAGC 
AAGGGCCCCT GTATCCTGCC CATCGTCCGT GACGGGCGTA AAGTCAGTAA CACCCACTAT 
TACCTTCTGC CAGAGAGGCC ACCGTATCTC GATCGATTCG ATCGCTTCTT CAGGGAGGCT 
GAAAGCCTTC CCGCCAACGA TGTGGAAGAA AAGCATGTAC GGCAAGCCAA CACAGCCACG 
GTCAGACCGA TGGTGGTCGG CAGTCACACA CTGCAGGGGC ACACCCAGGT GCAGGTCCAG 
CCGGGTGGTG ACCTGAAAGC CAATTTCTCC TCCAATAATA ACAGCAATCT GGGTTGGCCA 
CGGTCAGGGA TGAAGAGTTC AGTTAGTCTT CCTCGCGTCT GTTCAGACGG GCTGACAGCA 
CCAGCAGTCA CTGCTTTCTG TACCAGGACA GACGGAGGAG CGAACTCAGC TGATAGGGTC 
AAGATGGTGC AGGAGGCGGT TCACGGTGTG ACGATGGAGG AGTGCCAAGC CGCACTCCAC 
AACCACAACT GGAATGTCCA GCAAGCTGTG CATTATCTAA AGGTAGAACA GTTGTTCTGT 
TTGGGCCTGA GGAGCAGATC AGAGTGTCTA AAGCTGCTGG AGATGTGTGA CTGGAACCTG 
GAGGTGGCCA GCACTCACAT GCTGGATAAC TATGGATCTA CAACACGACA AAGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_025900625.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_025756410.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X5, mRNA.

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CloneID	OOr178727
Clone ID Related Accession (Same CDS sequence)	XM_025900626.1 , XM_025900625.1
Accession Version	XM_025900625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATGTG AGGAAGGGAC GGAATGGTTG CTGGAACTGC TGATGGAAGT GCAGCTGCAG 
CAGTACTTCT TACGGATCCG AGATGACCTT AATGTCACGC GGCTGTCGCA CTTTGACTAC 
GTCAAGAATG AAGACCTGGA GAAAATCGGC ATGGGTCGAC CTGGGCAGAG AAGGCTGTGG 
GAGGCCGTGA AAAGGAGGAA AGCCATGTGC AAGCGCAAGT CCTGGATGAG CAAGGTTTTT 
AGTGGTAAAC GTCCAGATGG AGGAGACGTG CCCCAGCAGG GTCAACCGGC TTCCTCGTTT 
CGTAAGCTGT CTCCCACACC ACCTCTGGGC GTCGGGGAGG GAGTCCTAGT CACGCAGCCC 
AGCGGTGGCG CTCTCCTCGA TGGGCAGCAG CAGGGTCTGA CCTGCCTCAT CCCAGAGAAG 
GACTTGACGC TGTTTGAGAA GCTGGGTGAC GGCTCATTTG GGGTCGTGAA GAGGGGAGAG 
TGGTTGACAC CTGCGGGGAA GGTGCTGAAC GTGGCCGTGA AGTGTCTGAA GACAGATGTG 
CTCAGCCAGC CCGACGCTCT GGAGGATTTC ATCTGCGAGG TCAACGTCAT GCACTCGCTG 
GACCATCAAA ACCTGATCCG CCTCTACGGT GTGGTGCTCA CACACCCAAT GAAGATGGTG 
ACTGAACTGG CACCTCTCGG TTCTCTGCTG GAGCGTTTGC GCTGCGTTCG TCCTCAAGGC 
CCAGTGTTGA TCCACACTCT GTGTCAGTAT GCTGTGCAGG TGGCTTGTGG CATGGCTTAT 
CTGGAGCAGA GGAGGTTCAT CCACAGGGAC CTGGCAGCGA GGAACATCCT GCTGGCCGCG 
GCTCACAGAG TGAAGATTGG TGACTTTGGC CTGATGAGGG CGCTGCCTAA CAACCACGAG 
CACTATGTCA TGCAGGAGCA TCGCAAGGTC CCCTTTGCAT GGTGTGCCCC AGAGAGTTTA 
AAGACCAGAA CCTTCTCCCA TGCAACAGAC ACTTGGATGT TTGGAGTCAC CCTCTGGGAG 
ATGTTCACAC ATGGTCAGGA GCCTTGGCTG GGTCTTAATG GTAGTCAGAT TCTCCATAAG 
ATTGATAAAG AGGGTGAACG CCTCCCTAAG CCAGAGGATT GTCCACAGGA TATCTATAAC 
GTCATGCTGC AGTGCTGGGC TCAGAAACCT GATGACAGAC CAACTTTTGT TGCTCTACGC 
GAGTTCCTGC TCGAGTCTAT GCCTACAGAC ATGTGTGCTC TCCAGGACTT TGATGAGCCT 
GACAAACTCC AGATCCAGGT CGATGACATT ATCACCATCA TAGAGGGGAG GGCAGAGAAT 
TATTGGTGGC GAGGTCAAAA TAAGCGCACC CTTAAGGTCG GGCAGTTCCC CAGGAATGTG 
GTGACTTCTG TAGCAGGGTT GTCTGCACAC GACATCAGCC GTCCACTCAA GAACAGCTTC 
ATTCACACAG GACATGGAGA CACCAACCCC CATCGCTGCT GGGGCTTCCC TGACAGGATC 
GATGATTTGT ACCTCGGGAA TCCCATGGAT CCTCCTGACG TTCTGGGCTT GGACCTCAGT 
GCTGCACAGC CCACACAGCT AGCAGGACGA GCTAAAAAGG AGCCTCCTCC CCGCCCTCCT 
CAACCAGCAG TGTTAATCAA GAAACCTTCC TATGACCCAG TAAATGAGGA GGAGGATCTG 
ACTTCTGCGG GACTAAAGAG ATTATCACTT CGGAAAACGG GTTCTGTCAA AGGCCTCAAA 
CTAAAACCGG CCGTGTGGGT CTCTGCTTCC AAACAGGGAG GTGGCAGGAC TTCAGGCTCA 
GGCCAGAACA CTAACAGTGA AGTGTCCCTC ATTGACTTTG GGGAAGAGTA CCCCATATCC 
ACACCGTCCC CCTCTCCCGT GGTTGAAATT CCAATTCCTC TATTGGCCAA GTTGGCTTTG 
GAAGCAGAAA ATCTCCTGGA CAGGACTCCA CCTCAAAGCC CATCCAAATC ATTGCCCCGC 
CCCCTTCACC CTACGCCAGT AGTGGACTGG GATGCGCGGC CATTACCCCC ACCCCCAGCT 
TATGACGATG TAGCGCAAGA TGAGGATGAT ATGGAGGTGA GCTCCATCAA CAGCTCGGAG 
CAGCAGAATG AGGCAGAGCA TAGTGATGGC CTTAATCCAG AAGAAACTCT GTCCTCTGGG 
CAAAAGGTCA ATGATGAGGC TCTAGTCTCC AGAGGTTCAG ACAGACCAGG TCTGGAAGAC 
AACCTGTTTC TCCCCAGCAA CCACAGCCAG GGCCTGTCCA CCTCTTTTTC ACAGTCTGCA 
GAGATCTTTC AAGAACTACA ACAAGAGTGC ATGAGGAGGC TCAATGTACC AGCAGCAAGT 
GCTCCTCGTT CGTGCTCGCC ATTGCAGAGC TCAGTCTTGT GTCCCCAGAC TCTTCAAAGT 
CCCCAAACCC ATGAGGGACA ACAGCAGAGT GTCCTCTCCT GTAACGAGGA CCGACCCCAG 
ATCCCCCCAC GAGTTCCCAT CCCCCCTCGC CCCATGAAGA AGGGGGACTA CACCTCTGGC 
CGCTGGTCAA GGGACCTCTC CCTTTCTCCA GTGCCGACTG AAACCACGGA AAACGTTTCA 
GGCGCAGGCC AGGAACGACC ACCTCAAATC CCTCCCAGAG ACCCTTTGTC TCAGCCTGGA 
TCAAGAACTC CAAGCCCAAT GGGTCTGGTA GTGGGTTCCC CCCAGCAGAG GCTCTACTCT 
GTCAGCCCCA ACACAATGCA GGCTGCCATT ACAACCTGCC CACCAACACA CACCTACAGC 
TCCTACCTCT CTACCTCTCC AGGTAAACTG ATGCCTACTA CACACAGCTT TGCTTCGGAC 
CCTAAATATG CTGCACCCAA AGTGATCCAG GCCCAGGTGC AGGGGAAGGA CGCTGCTAGC 
AAGGGCCCCT GTATCCTGCC CATCGTCCGT GACGGGCGTA AAGTCAGTAA CACCCACTAT 
TACCTTCTGC CAGAGAGGCC ACCGTATCTC GATCGATTCG ATCGCTTCTT CAGGGAGGCT 
GAAAGCCTTC CCGCCAACGA TGTGGAAGAA AAGCATGTAC GGCAAGCCAA CACAGCCACG 
GTCAGACCGA TGGTGGTCGG CAGTCACACA CTGCAGGGGC ACACCCAGGT GCAGGTCCAG 
CCGGGTGGTG ACCTGAAAGC CAATTTCTCC TCCAATAATA ACAGCAATCT GGGTTGGCCA 
CGGTCAGGGA TGAAGAGTTC AGTTAGTCTT CCTCGCGTCT GTTCAGACGG GCTGACAGCA 
CCAGCAGTCA CTGCTTTCTG TACCAGGACA GACGGAGGAG CGAACTCAGC TGATAGGGTC 
AAGATGGTGC AGGAGGCGGT TCACGGTGTG ACGATGGAGG AGTGCCAAGC CGCACTCCAC 
AACCACAACT GGAATGTCCA GCAAGCTGTG CATTATCTAA AGGTAGAACA GTTGTTCTGT 
TTGGGCCTGA GGAGCAGATC AGAGTGTCTA AAGCTGCTGG AGATGTGTGA CTGGAACCTG 
GAGGTGGCCA GCACTCACAT GCTGGATAAC TATGGATCTA CAACACGACA AAGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025756410.1
CDS	511..4050
Translation	MQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKEPPPRPPQPAVLIKKPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_025900625.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025900625.1

ORF Insert Sequence:

ATGCAATGTG AGGAAGGGAC GGAATGGTTG CTGGAACTGC TGATGGAAGT GCAGCTGCAG 
CAGTACTTCT TACGGATCCG AGATGACCTT AATGTCACGC GGCTGTCGCA CTTTGACTAC 
GTCAAGAATG AAGACCTGGA GAAAATCGGC ATGGGTCGAC CTGGGCAGAG AAGGCTGTGG 
GAGGCCGTGA AAAGGAGGAA AGCCATGTGC AAGCGCAAGT CCTGGATGAG CAAGGTTTTT 
AGTGGTAAAC GTCCAGATGG AGGAGACGTG CCCCAGCAGG GTCAACCGGC TTCCTCGTTT 
CGTAAGCTGT CTCCCACACC ACCTCTGGGC GTCGGGGAGG GAGTCCTAGT CACGCAGCCC 
AGCGGTGGCG CTCTCCTCGA TGGGCAGCAG CAGGGTCTGA CCTGCCTCAT CCCAGAGAAG 
GACTTGACGC TGTTTGAGAA GCTGGGTGAC GGCTCATTTG GGGTCGTGAA GAGGGGAGAG 
TGGTTGACAC CTGCGGGGAA GGTGCTGAAC GTGGCCGTGA AGTGTCTGAA GACAGATGTG 
CTCAGCCAGC CCGACGCTCT GGAGGATTTC ATCTGCGAGG TCAACGTCAT GCACTCGCTG 
GACCATCAAA ACCTGATCCG CCTCTACGGT GTGGTGCTCA CACACCCAAT GAAGATGGTG 
ACTGAACTGG CACCTCTCGG TTCTCTGCTG GAGCGTTTGC GCTGCGTTCG TCCTCAAGGC 
CCAGTGTTGA TCCACACTCT GTGTCAGTAT GCTGTGCAGG TGGCTTGTGG CATGGCTTAT 
CTGGAGCAGA GGAGGTTCAT CCACAGGGAC CTGGCAGCGA GGAACATCCT GCTGGCCGCG 
GCTCACAGAG TGAAGATTGG TGACTTTGGC CTGATGAGGG CGCTGCCTAA CAACCACGAG 
CACTATGTCA TGCAGGAGCA TCGCAAGGTC CCCTTTGCAT GGTGTGCCCC AGAGAGTTTA 
AAGACCAGAA CCTTCTCCCA TGCAACAGAC ACTTGGATGT TTGGAGTCAC CCTCTGGGAG 
ATGTTCACAC ATGGTCAGGA GCCTTGGCTG GGTCTTAATG GTAGTCAGAT TCTCCATAAG 
ATTGATAAAG AGGGTGAACG CCTCCCTAAG CCAGAGGATT GTCCACAGGA TATCTATAAC 
GTCATGCTGC AGTGCTGGGC TCAGAAACCT GATGACAGAC CAACTTTTGT TGCTCTACGC 
GAGTTCCTGC TCGAGTCTAT GCCTACAGAC ATGTGTGCTC TCCAGGACTT TGATGAGCCT 
GACAAACTCC AGATCCAGGT CGATGACATT ATCACCATCA TAGAGGGGAG GGCAGAGAAT 
TATTGGTGGC GAGGTCAAAA TAAGCGCACC CTTAAGGTCG GGCAGTTCCC CAGGAATGTG 
GTGACTTCTG TAGCAGGGTT GTCTGCACAC GACATCAGCC GTCCACTCAA GAACAGCTTC 
ATTCACACAG GACATGGAGA CACCAACCCC CATCGCTGCT GGGGCTTCCC TGACAGGATC 
GATGATTTGT ACCTCGGGAA TCCCATGGAT CCTCCTGACG TTCTGGGCTT GGACCTCAGT 
GCTGCACAGC CCACACAGCT AGCAGGACGA GCTAAAAAGG AGCCTCCTCC CCGCCCTCCT 
CAACCAGCAG TGTTAATCAA GAAACCTTCC TATGACCCAG TAAATGAGGA GGAGGATCTG 
ACTTCTGCGG GACTAAAGAG ATTATCACTT CGGAAAACGG GTTCTGTCAA AGGCCTCAAA 
CTAAAACCGG CCGTGTGGGT CTCTGCTTCC AAACAGGGAG GTGGCAGGAC TTCAGGCTCA 
GGCCAGAACA CTAACAGTGA AGTGTCCCTC ATTGACTTTG GGGAAGAGTA CCCCATATCC 
ACACCGTCCC CCTCTCCCGT GGTTGAAATT CCAATTCCTC TATTGGCCAA GTTGGCTTTG 
GAAGCAGAAA ATCTCCTGGA CAGGACTCCA CCTCAAAGCC CATCCAAATC ATTGCCCCGC 
CCCCTTCACC CTACGCCAGT AGTGGACTGG GATGCGCGGC CATTACCCCC ACCCCCAGCT 
TATGACGATG TAGCGCAAGA TGAGGATGAT ATGGAGGTGA GCTCCATCAA CAGCTCGGAG 
CAGCAGAATG AGGCAGAGCA TAGTGATGGC CTTAATCCAG AAGAAACTCT GTCCTCTGGG 
CAAAAGGTCA ATGATGAGGC TCTAGTCTCC AGAGGTTCAG ACAGACCAGG TCTGGAAGAC 
AACCTGTTTC TCCCCAGCAA CCACAGCCAG GGCCTGTCCA CCTCTTTTTC ACAGTCTGCA 
GAGATCTTTC AAGAACTACA ACAAGAGTGC ATGAGGAGGC TCAATGTACC AGCAGCAAGT 
GCTCCTCGTT CGTGCTCGCC ATTGCAGAGC TCAGTCTTGT GTCCCCAGAC TCTTCAAAGT 
CCCCAAACCC ATGAGGGACA ACAGCAGAGT GTCCTCTCCT GTAACGAGGA CCGACCCCAG 
ATCCCCCCAC GAGTTCCCAT CCCCCCTCGC CCCATGAAGA AGGGGGACTA CACCTCTGGC 
CGCTGGTCAA GGGACCTCTC CCTTTCTCCA GTGCCGACTG AAACCACGGA AAACGTTTCA 
GGCGCAGGCC AGGAACGACC ACCTCAAATC CCTCCCAGAG ACCCTTTGTC TCAGCCTGGA 
TCAAGAACTC CAAGCCCAAT GGGTCTGGTA GTGGGTTCCC CCCAGCAGAG GCTCTACTCT 
GTCAGCCCCA ACACAATGCA GGCTGCCATT ACAACCTGCC CACCAACACA CACCTACAGC 
TCCTACCTCT CTACCTCTCC AGGTAAACTG ATGCCTACTA CACACAGCTT TGCTTCGGAC 
CCTAAATATG CTGCACCCAA AGTGATCCAG GCCCAGGTGC AGGGGAAGGA CGCTGCTAGC 
AAGGGCCCCT GTATCCTGCC CATCGTCCGT GACGGGCGTA AAGTCAGTAA CACCCACTAT 
TACCTTCTGC CAGAGAGGCC ACCGTATCTC GATCGATTCG ATCGCTTCTT CAGGGAGGCT 
GAAAGCCTTC CCGCCAACGA TGTGGAAGAA AAGCATGTAC GGCAAGCCAA CACAGCCACG 
GTCAGACCGA TGGTGGTCGG CAGTCACACA CTGCAGGGGC ACACCCAGGT GCAGGTCCAG 
CCGGGTGGTG ACCTGAAAGC CAATTTCTCC TCCAATAATA ACAGCAATCT GGGTTGGCCA 
CGGTCAGGGA TGAAGAGTTC AGTTAGTCTT CCTCGCGTCT GTTCAGACGG GCTGACAGCA 
CCAGCAGTCA CTGCTTTCTG TACCAGGACA GACGGAGGAG CGAACTCAGC TGATAGGGTC 
AAGATGGTGC AGGAGGCGGT TCACGGTGTG ACGATGGAGG AGTGCCAAGC CGCACTCCAC 
AACCACAACT GGAATGTCCA GCAAGCTGTG CATTATCTAA AGGTAGAACA GTTGTTCTGT 
TTGGGCCTGA GGAGCAGATC AGAGTGTCTA AAGCTGCTGG AGATGTGTGA CTGGAACCTG 
GAGGTGGCCA GCACTCACAT GCTGGATAAC TATGGATCTA CAACACGACA AAGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_019347434.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019202979.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr45856
Clone ID Related Accession (Same CDS sequence)	XM_019347434.1
Accession Version	XM_019347434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAGGAGCC TCCTCCCCGC CCTCCTCAAC CAGCAGTGTT AATCAAGAGT 
AAGTCTGGTT TCTCTCAGCA GCTCTTCACC CATTGCTCCT GCCCTCTCTG TTCCCTCCTA 
GCTCCACTCC TCAAAGAACC TTCCTATGAC CCAGTAAATG AGGAGGAGGA TCTGACTTCT 
GCGGGACTAA AGAGATTATC ACTTCGGAAA ACGGGTTCTG TCAAAGGCCT CAAACTAAAA 
CCGGCCGTGT GGGTCTCTGC TTCCAAACAG GGAGGTGGCA GGACTTCAGG CTCAGGCCAG 
AACACTAACA GTGAAGTGTC CCTCATTGAC TTTGGGGAAG AGTACCCCAT ATCCACACCG 
TCCCCCTCTC CCGTGGTTGA AATTCCAATT CCTCTATTGG CCAAGTTGGC TTTGGAAGCA 
GAAAATCTCC TGGACAGGAC TCCACCTCAA AGCCCATCCA AATCATTGCC CCGCCCCCTT 
CACCCTACGC CAGTAGTGGA CTGGGATGCG CGGCCATTAC CCCCACCCCC AGCTTATGAC 
GATGTAGCGC AAGATGAGGA TGATATGGAG GTGAGCTCCA TCAACAGCTC GGAGCAGCAG 
AATGAGGCAG AGCATAGTGA TGGCCTTAAT CCAGAAGAAA CTCTGTCCTC TGGGCAAAAG 
GTCAATGATG AGGCTCTAGT CTCCAGAGGT TCAGACAGAC CAGGTCTGGA AGACAACCTG 
TTTCTCCCCA GCAACCACAG CCAGGGCCTG TCCACCTCTT TTTCACAGTC TGCAGAGATC 
TTTCAAGAAC TACAACAAGA GTGCATGAGG AGGCTCAATG TACCAGCAGC AAGTGCTCCT 
CGTTCGTGCT CGCCATTGCA GAGCTCAGTC TTGTGTCCCC AGACTCTTCA AAGTCCCCAA 
ACCCATGAGG GACAACAGCA GAGTGTCCTC TCCTGTAACG AGGACCGACC CCAGATCCCC 
CCACGAGTTC CCATCCCCCC TCGCCCCATG AAGAAGGGGG ACTACACCTC TGGCCGCTGG 
TCAAGGGACC TCTCCCTTTC TCCAGTGCCG ACTGAAACCA CGGAAAACGT TTCAGGCGCA 
GGCCAGGAAC GACCACCTCA AATCCCTCCC AGAGACCCTT TGTCTCAGCC TGGATCAAGA 
ACTCCAAGCC CAATGGGTCT GGTAGTGGGT TCCCCCCAGC AGAGGCTCTA CTCTGTCAGC 
CCCAACACAA TGCAGGCTGC CATTACAACC TGCCCACCAA CACACACCTA CAGCTCCTAC 
CTCTCTACCT CTCCAGGTAA ACTGATGCCT ACTACACACA GCTTTGCTTC GGACCCTAAA 
TATGCTGCAC CCAAAGTGAT CCAGGCCCAG GTGCAGGGGA AGGACGCTGC TAGCAAGGGC 
CCCTGTATCC TGCCCATCGT CCGTGACGGG CGTAAAGTCA GTAACACCCA CTATTACCTT 
CTGCCAGAGA GGCCACCGTA TCTCGATCGA TTCGATCGCT TCTTCAGGGA GGCTGAAAGC 
CTTCCCGCCA ACGATGTGGA AGAAAAGCAT GTACGGCAAG CCAACACAGC CACGGTCAGA 
CCGATGGTGG TCGGCAGTCA CACACTGCAG GGGCACACCC AGGTGCAGGT CCAGCCGGGT 
GGTGACCTGA AAGCCAATTT CTCCTCCAAT AATAACAGCA ATCTGGGTTG GCCACGGTCA 
GGGATGAAGA GTTCAGTTAG TCTTCCTCGC GTCTGTTCAG ACGGGCTGAC AGCACCAGCA 
GTCACTGCTT TCTGTACCAG GACAGACGGA GGAGCGAACT CAGCTGATAG GGTCAAGATG 
GTGCAGGAGG CGGTTCACGG TGTGACGATG GAGGAGTGCC AAGCCGCACT CCACAACCAC 
AACTGGAATG TCCAGCAAGC TGTGCATTAT CTAAAGGTAG AACAGTTGTT CTGTTTGGGC 
CTGAGGAGCA GATCAGAGTG TCTAAAGCTG CTGGAGATGT GTGACTGGAA CCTGGAGGTG 
GCCAGCACTC ACATGCTGGA TAACTATGGA TCTACAACAC GACAAAGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019202979.1
CDS	1334..5047
Translation	MGETAEYQRLQETTESDYQRLPSDDEEKLGSSMQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKEPPPRPPQPAVLIKSKSGFSQQLFTHCSCPLCSLLAPLLKEPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_019347434.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019347434.1

ORF Insert Sequence:

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAGGAGCC TCCTCCCCGC CCTCCTCAAC CAGCAGTGTT AATCAAGAGT 
AAGTCTGGTT TCTCTCAGCA GCTCTTCACC CATTGCTCCT GCCCTCTCTG TTCCCTCCTA 
GCTCCACTCC TCAAAGAACC TTCCTATGAC CCAGTAAATG AGGAGGAGGA TCTGACTTCT 
GCGGGACTAA AGAGATTATC ACTTCGGAAA ACGGGTTCTG TCAAAGGCCT CAAACTAAAA 
CCGGCCGTGT GGGTCTCTGC TTCCAAACAG GGAGGTGGCA GGACTTCAGG CTCAGGCCAG 
AACACTAACA GTGAAGTGTC CCTCATTGAC TTTGGGGAAG AGTACCCCAT ATCCACACCG 
TCCCCCTCTC CCGTGGTTGA AATTCCAATT CCTCTATTGG CCAAGTTGGC TTTGGAAGCA 
GAAAATCTCC TGGACAGGAC TCCACCTCAA AGCCCATCCA AATCATTGCC CCGCCCCCTT 
CACCCTACGC CAGTAGTGGA CTGGGATGCG CGGCCATTAC CCCCACCCCC AGCTTATGAC 
GATGTAGCGC AAGATGAGGA TGATATGGAG GTGAGCTCCA TCAACAGCTC GGAGCAGCAG 
AATGAGGCAG AGCATAGTGA TGGCCTTAAT CCAGAAGAAA CTCTGTCCTC TGGGCAAAAG 
GTCAATGATG AGGCTCTAGT CTCCAGAGGT TCAGACAGAC CAGGTCTGGA AGACAACCTG 
TTTCTCCCCA GCAACCACAG CCAGGGCCTG TCCACCTCTT TTTCACAGTC TGCAGAGATC 
TTTCAAGAAC TACAACAAGA GTGCATGAGG AGGCTCAATG TACCAGCAGC AAGTGCTCCT 
CGTTCGTGCT CGCCATTGCA GAGCTCAGTC TTGTGTCCCC AGACTCTTCA AAGTCCCCAA 
ACCCATGAGG GACAACAGCA GAGTGTCCTC TCCTGTAACG AGGACCGACC CCAGATCCCC 
CCACGAGTTC CCATCCCCCC TCGCCCCATG AAGAAGGGGG ACTACACCTC TGGCCGCTGG 
TCAAGGGACC TCTCCCTTTC TCCAGTGCCG ACTGAAACCA CGGAAAACGT TTCAGGCGCA 
GGCCAGGAAC GACCACCTCA AATCCCTCCC AGAGACCCTT TGTCTCAGCC TGGATCAAGA 
ACTCCAAGCC CAATGGGTCT GGTAGTGGGT TCCCCCCAGC AGAGGCTCTA CTCTGTCAGC 
CCCAACACAA TGCAGGCTGC CATTACAACC TGCCCACCAA CACACACCTA CAGCTCCTAC 
CTCTCTACCT CTCCAGGTAA ACTGATGCCT ACTACACACA GCTTTGCTTC GGACCCTAAA 
TATGCTGCAC CCAAAGTGAT CCAGGCCCAG GTGCAGGGGA AGGACGCTGC TAGCAAGGGC 
CCCTGTATCC TGCCCATCGT CCGTGACGGG CGTAAAGTCA GTAACACCCA CTATTACCTT 
CTGCCAGAGA GGCCACCGTA TCTCGATCGA TTCGATCGCT TCTTCAGGGA GGCTGAAAGC 
CTTCCCGCCA ACGATGTGGA AGAAAAGCAT GTACGGCAAG CCAACACAGC CACGGTCAGA 
CCGATGGTGG TCGGCAGTCA CACACTGCAG GGGCACACCC AGGTGCAGGT CCAGCCGGGT 
GGTGACCTGA AAGCCAATTT CTCCTCCAAT AATAACAGCA ATCTGGGTTG GCCACGGTCA 
GGGATGAAGA GTTCAGTTAG TCTTCCTCGC GTCTGTTCAG ACGGGCTGAC AGCACCAGCA 
GTCACTGCTT TCTGTACCAG GACAGACGGA GGAGCGAACT CAGCTGATAG GGTCAAGATG 
GTGCAGGAGG CGGTTCACGG TGTGACGATG GAGGAGTGCC AAGCCGCACT CCACAACCAC 
AACTGGAATG TCCAGCAAGC TGTGCATTAT CTAAAGGTAG AACAGTTGTT CTGTTTGGGC 
CTGAGGAGCA GATCAGAGTG TCTAAAGCTG CTGGAGATGT GTGACTGGAA CCTGGAGGTG 
GCCAGCACTC ACATGCTGGA TAACTATGGA TCTACAACAC GACAAAGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_019347435.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019202980.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X2, mRNA.

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CloneID	OOr45857
Clone ID Related Accession (Same CDS sequence)	XM_019347435.1
Accession Version	XM_019347435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCT TTGACAAGCT GAAAAAGACA TTCCCCTTCC TGGCACGCTT TCACGTATAT 
CGAAAACTGG GCAGCAGCAT GCAATGTGAG GAAGGGACGG AATGGTTGCT GGAACTGCTG 
ATGGAAGTGC AGCTGCAGCA GTACTTCTTA CGGATCCGAG ATGACCTTAA TGTCACGCGG 
CTGTCGCACT TTGACTACGT CAAGAATGAA GACCTGGAGA AAATCGGCAT GGGTCGACCT 
GGGCAGAGAA GGCTGTGGGA GGCCGTGAAA AGGAGGAAAG CCATGTGCAA GCGCAAGTCC 
TGGATGAGCA AGGTTTTTAG TGGTAAACGT CCAGATGGAG GAGACGTGCC CCAGCAGGGT 
CAACCGGCTT CCTCGTTTCG TAAGCTGTCT CCCACACCAC CTCTGGGCGT CGGGGAGGGA 
GTCCTAGTCA CGCAGCCCAG CGGTGGCGCT CTCCTCGATG GGCAGCAGCA GGGTCTGACC 
TGCCTCATCC CAGAGAAGGA CTTGACGCTG TTTGAGAAGC TGGGTGACGG CTCATTTGGG 
GTCGTGAAGA GGGGAGAGTG GTTGACACCT GCGGGGAAGG TGCTGAACGT GGCCGTGAAG 
TGTCTGAAGA CAGATGTGCT CAGCCAGCCC GACGCTCTGG AGGATTTCAT CTGCGAGGTC 
AACGTCATGC ACTCGCTGGA CCATCAAAAC CTGATCCGCC TCTACGGTGT GGTGCTCACA 
CACCCAATGA AGATGGTGAC TGAACTGGCA CCTCTCGGTT CTCTGCTGGA GCGTTTGCGC 
TGCGTTCGTC CTCAAGGCCC AGTGTTGATC CACACTCTGT GTCAGTATGC TGTGCAGGTG 
GCTTGTGGCA TGGCTTATCT GGAGCAGAGG AGGTTCATCC ACAGGGACCT GGCAGCGAGG 
AACATCCTGC TGGCCGCGGC TCACAGAGTG AAGATTGGTG ACTTTGGCCT GATGAGGGCG 
CTGCCTAACA ACCACGAGCA CTATGTCATG CAGGAGCATC GCAAGGTCCC CTTTGCATGG 
TGTGCCCCAG AGAGTTTAAA GACCAGAACC TTCTCCCATG CAACAGACAC TTGGATGTTT 
GGAGTCACCC TCTGGGAGAT GTTCACACAT GGTCAGGAGC CTTGGCTGGG TCTTAATGGT 
AGTCAGATTC TCCATAAGAT TGATAAAGAG GGTGAACGCC TCCCTAAGCC AGAGGATTGT 
CCACAGGATA TCTATAACGT CATGCTGCAG TGCTGGGCTC AGAAACCTGA TGACAGACCA 
ACTTTTGTTG CTCTACGCGA GTTCCTGCTC GAGTCTATGC CTACAGACAT GTGTGCTCTC 
CAGGACTTTG ATGAGCCTGA CAAACTCCAG ATCCAGGTCG ATGACATTAT CACCATCATA 
GAGGGGAGGG CAGAGAATTA TTGGTGGCGA GGTCAAAATA AGCGCACCCT TAAGGTCGGG 
CAGTTCCCCA GGAATGTGGT GACTTCTGTA GCAGGGTTGT CTGCACACGA CATCAGCCGT 
CCACTCAAGA ACAGCTTCAT TCACACAGGA CATGGAGACA CCAACCCCCA TCGCTGCTGG 
GGCTTCCCTG ACAGGATCGA TGATTTGTAC CTCGGGAATC CCATGGATCC TCCTGACGTT 
CTGGGCTTGG ACCTCAGTGC TGCACAGCCC ACACAGCTAG CAGGACGAGC TAAAAAGGAG 
CCTCCTCCCC GCCCTCCTCA ACCAGCAGTG TTAATCAAGA GTAAGTCTGG TTTCTCTCAG 
CAGCTCTTCA CCCATTGCTC CTGCCCTCTC TGTTCCCTCC TAGCTCCACT CCTCAAAGAA 
CCTTCCTATG ACCCAGTAAA TGAGGAGGAG GATCTGACTT CTGCGGGACT AAAGAGATTA 
TCACTTCGGA AAACGGGTTC TGTCAAAGGC CTCAAACTAA AACCGGCCGT GTGGGTCTCT 
GCTTCCAAAC AGGGAGGTGG CAGGACTTCA GGCTCAGGCC AGAACACTAA CAGTGAAGTG 
TCCCTCATTG ACTTTGGGGA AGAGTACCCC ATATCCACAC CGTCCCCCTC TCCCGTGGTT 
GAAATTCCAA TTCCTCTATT GGCCAAGTTG GCTTTGGAAG CAGAAAATCT CCTGGACAGG 
ACTCCACCTC AAAGCCCATC CAAATCATTG CCCCGCCCCC TTCACCCTAC GCCAGTAGTG 
GACTGGGATG CGCGGCCATT ACCCCCACCC CCAGCTTATG ACGATGTAGC GCAAGATGAG 
GATGATATGG AGGTGAGCTC CATCAACAGC TCGGAGCAGC AGAATGAGGC AGAGCATAGT 
GATGGCCTTA ATCCAGAAGA AACTCTGTCC TCTGGGCAAA AGGTCAATGA TGAGGCTCTA 
GTCTCCAGAG GTTCAGACAG ACCAGGTCTG GAAGACAACC TGTTTCTCCC CAGCAACCAC 
AGCCAGGGCC TGTCCACCTC TTTTTCACAG TCTGCAGAGA TCTTTCAAGA ACTACAACAA 
GAGTGCATGA GGAGGCTCAA TGTACCAGCA GCAAGTGCTC CTCGTTCGTG CTCGCCATTG 
CAGAGCTCAG TCTTGTGTCC CCAGACTCTT CAAAGTCCCC AAACCCATGA GGGACAACAG 
CAGAGTGTCC TCTCCTGTAA CGAGGACCGA CCCCAGATCC CCCCACGAGT TCCCATCCCC 
CCTCGCCCCA TGAAGAAGGG GGACTACACC TCTGGCCGCT GGTCAAGGGA CCTCTCCCTT 
TCTCCAGTGC CGACTGAAAC CACGGAAAAC GTTTCAGGCG CAGGCCAGGA ACGACCACCT 
CAAATCCCTC CCAGAGACCC TTTGTCTCAG CCTGGATCAA GAACTCCAAG CCCAATGGGT 
CTGGTAGTGG GTTCCCCCCA GCAGAGGCTC TACTCTGTCA GCCCCAACAC AATGCAGGCT 
GCCATTACAA CCTGCCCACC AACACACACC TACAGCTCCT ACCTCTCTAC CTCTCCAGGT 
AAACTGATGC CTACTACACA CAGCTTTGCT TCGGACCCTA AATATGCTGC ACCCAAAGTG 
ATCCAGGCCC AGGTGCAGGG GAAGGACGCT GCTAGCAAGG GCCCCTGTAT CCTGCCCATC 
GTCCGTGACG GGCGTAAAGT CAGTAACACC CACTATTACC TTCTGCCAGA GAGGCCACCG 
TATCTCGATC GATTCGATCG CTTCTTCAGG GAGGCTGAAA GCCTTCCCGC CAACGATGTG 
GAAGAAAAGC ATGTACGGCA AGCCAACACA GCCACGGTCA GACCGATGGT GGTCGGCAGT 
CACACACTGC AGGGGCACAC CCAGGTGCAG GTCCAGCCGG GTGGTGACCT GAAAGCCAAT 
TTCTCCTCCA ATAATAACAG CAATCTGGGT TGGCCACGGT CAGGGATGAA GAGTTCAGTT 
AGTCTTCCTC GCGTCTGTTC AGACGGGCTG ACAGCACCAG CAGTCACTGC TTTCTGTACC 
AGGACAGACG GAGGAGCGAA CTCAGCTGAT AGGGTCAAGA TGGTGCAGGA GGCGGTTCAC 
GGTGTGACGA TGGAGGAGTG CCAAGCCGCA CTCCACAACC ACAACTGGAA TGTCCAGCAA 
GCTGTGCATT ATCTAAAGGT AGAACAGTTG TTCTGTTTGG GCCTGAGGAG CAGATCAGAG 
TGTCTAAAGC TGCTGGAGAT GTGTGACTGG AACCTGGAGG TGGCCAGCAC TCACATGCTG 
GATAACTATG GATCTACAAC ACGACAAAGA CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019202980.1
CDS	513..4208
Translation	MRRFDKLKKTFPFLARFHVYRKLGSSMQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKEPPPRPPQPAVLIKSKSGFSQQLFTHCSCPLCSLLAPLLKEPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_019347435.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019347435.1

ORF Insert Sequence:

ATGCGACGCT TTGACAAGCT GAAAAAGACA TTCCCCTTCC TGGCACGCTT TCACGTATAT 
CGAAAACTGG GCAGCAGCAT GCAATGTGAG GAAGGGACGG AATGGTTGCT GGAACTGCTG 
ATGGAAGTGC AGCTGCAGCA GTACTTCTTA CGGATCCGAG ATGACCTTAA TGTCACGCGG 
CTGTCGCACT TTGACTACGT CAAGAATGAA GACCTGGAGA AAATCGGCAT GGGTCGACCT 
GGGCAGAGAA GGCTGTGGGA GGCCGTGAAA AGGAGGAAAG CCATGTGCAA GCGCAAGTCC 
TGGATGAGCA AGGTTTTTAG TGGTAAACGT CCAGATGGAG GAGACGTGCC CCAGCAGGGT 
CAACCGGCTT CCTCGTTTCG TAAGCTGTCT CCCACACCAC CTCTGGGCGT CGGGGAGGGA 
GTCCTAGTCA CGCAGCCCAG CGGTGGCGCT CTCCTCGATG GGCAGCAGCA GGGTCTGACC 
TGCCTCATCC CAGAGAAGGA CTTGACGCTG TTTGAGAAGC TGGGTGACGG CTCATTTGGG 
GTCGTGAAGA GGGGAGAGTG GTTGACACCT GCGGGGAAGG TGCTGAACGT GGCCGTGAAG 
TGTCTGAAGA CAGATGTGCT CAGCCAGCCC GACGCTCTGG AGGATTTCAT CTGCGAGGTC 
AACGTCATGC ACTCGCTGGA CCATCAAAAC CTGATCCGCC TCTACGGTGT GGTGCTCACA 
CACCCAATGA AGATGGTGAC TGAACTGGCA CCTCTCGGTT CTCTGCTGGA GCGTTTGCGC 
TGCGTTCGTC CTCAAGGCCC AGTGTTGATC CACACTCTGT GTCAGTATGC TGTGCAGGTG 
GCTTGTGGCA TGGCTTATCT GGAGCAGAGG AGGTTCATCC ACAGGGACCT GGCAGCGAGG 
AACATCCTGC TGGCCGCGGC TCACAGAGTG AAGATTGGTG ACTTTGGCCT GATGAGGGCG 
CTGCCTAACA ACCACGAGCA CTATGTCATG CAGGAGCATC GCAAGGTCCC CTTTGCATGG 
TGTGCCCCAG AGAGTTTAAA GACCAGAACC TTCTCCCATG CAACAGACAC TTGGATGTTT 
GGAGTCACCC TCTGGGAGAT GTTCACACAT GGTCAGGAGC CTTGGCTGGG TCTTAATGGT 
AGTCAGATTC TCCATAAGAT TGATAAAGAG GGTGAACGCC TCCCTAAGCC AGAGGATTGT 
CCACAGGATA TCTATAACGT CATGCTGCAG TGCTGGGCTC AGAAACCTGA TGACAGACCA 
ACTTTTGTTG CTCTACGCGA GTTCCTGCTC GAGTCTATGC CTACAGACAT GTGTGCTCTC 
CAGGACTTTG ATGAGCCTGA CAAACTCCAG ATCCAGGTCG ATGACATTAT CACCATCATA 
GAGGGGAGGG CAGAGAATTA TTGGTGGCGA GGTCAAAATA AGCGCACCCT TAAGGTCGGG 
CAGTTCCCCA GGAATGTGGT GACTTCTGTA GCAGGGTTGT CTGCACACGA CATCAGCCGT 
CCACTCAAGA ACAGCTTCAT TCACACAGGA CATGGAGACA CCAACCCCCA TCGCTGCTGG 
GGCTTCCCTG ACAGGATCGA TGATTTGTAC CTCGGGAATC CCATGGATCC TCCTGACGTT 
CTGGGCTTGG ACCTCAGTGC TGCACAGCCC ACACAGCTAG CAGGACGAGC TAAAAAGGAG 
CCTCCTCCCC GCCCTCCTCA ACCAGCAGTG TTAATCAAGA GTAAGTCTGG TTTCTCTCAG 
CAGCTCTTCA CCCATTGCTC CTGCCCTCTC TGTTCCCTCC TAGCTCCACT CCTCAAAGAA 
CCTTCCTATG ACCCAGTAAA TGAGGAGGAG GATCTGACTT CTGCGGGACT AAAGAGATTA 
TCACTTCGGA AAACGGGTTC TGTCAAAGGC CTCAAACTAA AACCGGCCGT GTGGGTCTCT 
GCTTCCAAAC AGGGAGGTGG CAGGACTTCA GGCTCAGGCC AGAACACTAA CAGTGAAGTG 
TCCCTCATTG ACTTTGGGGA AGAGTACCCC ATATCCACAC CGTCCCCCTC TCCCGTGGTT 
GAAATTCCAA TTCCTCTATT GGCCAAGTTG GCTTTGGAAG CAGAAAATCT CCTGGACAGG 
ACTCCACCTC AAAGCCCATC CAAATCATTG CCCCGCCCCC TTCACCCTAC GCCAGTAGTG 
GACTGGGATG CGCGGCCATT ACCCCCACCC CCAGCTTATG ACGATGTAGC GCAAGATGAG 
GATGATATGG AGGTGAGCTC CATCAACAGC TCGGAGCAGC AGAATGAGGC AGAGCATAGT 
GATGGCCTTA ATCCAGAAGA AACTCTGTCC TCTGGGCAAA AGGTCAATGA TGAGGCTCTA 
GTCTCCAGAG GTTCAGACAG ACCAGGTCTG GAAGACAACC TGTTTCTCCC CAGCAACCAC 
AGCCAGGGCC TGTCCACCTC TTTTTCACAG TCTGCAGAGA TCTTTCAAGA ACTACAACAA 
GAGTGCATGA GGAGGCTCAA TGTACCAGCA GCAAGTGCTC CTCGTTCGTG CTCGCCATTG 
CAGAGCTCAG TCTTGTGTCC CCAGACTCTT CAAAGTCCCC AAACCCATGA GGGACAACAG 
CAGAGTGTCC TCTCCTGTAA CGAGGACCGA CCCCAGATCC CCCCACGAGT TCCCATCCCC 
CCTCGCCCCA TGAAGAAGGG GGACTACACC TCTGGCCGCT GGTCAAGGGA CCTCTCCCTT 
TCTCCAGTGC CGACTGAAAC CACGGAAAAC GTTTCAGGCG CAGGCCAGGA ACGACCACCT 
CAAATCCCTC CCAGAGACCC TTTGTCTCAG CCTGGATCAA GAACTCCAAG CCCAATGGGT 
CTGGTAGTGG GTTCCCCCCA GCAGAGGCTC TACTCTGTCA GCCCCAACAC AATGCAGGCT 
GCCATTACAA CCTGCCCACC AACACACACC TACAGCTCCT ACCTCTCTAC CTCTCCAGGT 
AAACTGATGC CTACTACACA CAGCTTTGCT TCGGACCCTA AATATGCTGC ACCCAAAGTG 
ATCCAGGCCC AGGTGCAGGG GAAGGACGCT GCTAGCAAGG GCCCCTGTAT CCTGCCCATC 
GTCCGTGACG GGCGTAAAGT CAGTAACACC CACTATTACC TTCTGCCAGA GAGGCCACCG 
TATCTCGATC GATTCGATCG CTTCTTCAGG GAGGCTGAAA GCCTTCCCGC CAACGATGTG 
GAAGAAAAGC ATGTACGGCA AGCCAACACA GCCACGGTCA GACCGATGGT GGTCGGCAGT 
CACACACTGC AGGGGCACAC CCAGGTGCAG GTCCAGCCGG GTGGTGACCT GAAAGCCAAT 
TTCTCCTCCA ATAATAACAG CAATCTGGGT TGGCCACGGT CAGGGATGAA GAGTTCAGTT 
AGTCTTCCTC GCGTCTGTTC AGACGGGCTG ACAGCACCAG CAGTCACTGC TTTCTGTACC 
AGGACAGACG GAGGAGCGAA CTCAGCTGAT AGGGTCAAGA TGGTGCAGGA GGCGGTTCAC 
GGTGTGACGA TGGAGGAGTG CCAAGCCGCA CTCCACAACC ACAACTGGAA TGTCCAGCAA 
GCTGTGCATT ATCTAAAGGT AGAACAGTTG TTCTGTTTGG GCCTGAGGAG CAGATCAGAG 
TGTCTAAAGC TGCTGGAGAT GTGTGACTGG AACCTGGAGG TGGCCAGCAC TCACATGCTG 
GATAACTATG GATCTACAAC ACGACAAAGA CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_005458090.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005458147.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X3, mRNA.

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CloneID	OOr45858
Clone ID Related Accession (Same CDS sequence)	XM_005458090.3 , XM_005458090.4
Accession Version	XM_005458090.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_005458090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAGGAGCC TCCTCCCCGC CCTCCTCAAC CAGCAGTGTT AATCAAGAAA 
CCTTCCTATG ACCCAGTAAA TGAGGAGGAG GATCTGACTT CTGCGGGACT AAAGAGATTA 
TCACTTCGGA AAACGGGTTC TGTCAAAGGC CTCAAACTAA AACCGGCCGT GTGGGTCTCT 
GCTTCCAAAC AGGGAGGTGG CAGGACTTCA GGCTCAGGCC AGAACACTAA CAGTGAAGTG 
TCCCTCATTG ACTTTGGGGA AGAGTACCCC ATATCCACAC CGTCCCCCTC TCCCGTGGTT 
GAAATTCCAA TTCCTCTATT GGCCAAGTTG GCTTTGGAAG CAGAAAATCT CCTGGACAGG 
ACTCCACCTC AAAGCCCATC CAAATCATTG CCCCGCCCCC TTCACCCTAC GCCAGTAGTG 
GACTGGGATG CGCGGCCATT ACCCCCACCC CCAGCTTATG ACGATGTAGC GCAAGATGAG 
GATGATATGG AGGTGAGCTC CATCAACAGC TCGGAGCAGC AGAATGAGGC AGAGCATAGT 
GATGGCCTTA ATCCAGAAGA AACTCTGTCC TCTGGGCAAA AGGTCAATGA TGAGGCTCTA 
GTCTCCAGAG GTTCAGACAG ACCAGGTCTG GAAGACAACC TGTTTCTCCC CAGCAACCAC 
AGCCAGGGCC TGTCCACCTC TTTTTCACAG TCTGCAGAGA TCTTTCAAGA ACTACAACAA 
GAGTGCATGA GGAGGCTCAA TGTACCAGCA GCAAGTGCTC CTCGTTCGTG CTCGCCATTG 
CAGAGCTCAG TCTTGTGTCC CCAGACTCTT CAAAGTCCCC AAACCCATGA GGGACAACAG 
CAGAGTGTCC TCTCCTGTAA CGAGGACCGA CCCCAGATCC CCCCACGAGT TCCCATCCCC 
CCTCGCCCCA TGAAGAAGGG GGACTACACC TCTGGCCGCT GGTCAAGGGA CCTCTCCCTT 
TCTCCAGTGC CGACTGAAAC CACGGAAAAC GTTTCAGGCG CAGGCCAGGA ACGACCACCT 
CAAATCCCTC CCAGAGACCC TTTGTCTCAG CCTGGATCAA GAACTCCAAG CCCAATGGGT 
CTGGTAGTGG GTTCCCCCCA GCAGAGGCTC TACTCTGTCA GCCCCAACAC AATGCAGGCT 
GCCATTACAA CCTGCCCACC AACACACACC TACAGCTCCT ACCTCTCTAC CTCTCCAGGT 
AAACTGATGC CTACTACACA CAGCTTTGCT TCGGACCCTA AATATGCTGC ACCCAAAGTG 
ATCCAGGCCC AGGTGCAGGG GAAGGACGCT GCTAGCAAGG GCCCCTGTAT CCTGCCCATC 
GTCCGTGACG GGCGTAAAGT CAGTAACACC CACTATTACC TTCTGCCAGA GAGGCCACCG 
TATCTCGATC GATTCGATCG CTTCTTCAGG GAGGCTGAAA GCCTTCCCGC CAACGATGTG 
GAAGAAAAGC ATGTACGGCA AGCCAACACA GCCACGGTCA GACCGATGGT GGTCGGCAGT 
CACACACTGC AGGGGCACAC CCAGGTGCAG GTCCAGCCGG GTGGTGACCT GAAAGCCAAT 
TTCTCCTCCA ATAATAACAG CAATCTGGGT TGGCCACGGT CAGGGATGAA GAGTTCAGTT 
AGTCTTCCTC GCGTCTGTTC AGACGGGCTG ACAGCACCAG CAGTCACTGC TTTCTGTACC 
AGGACAGACG GAGGAGCGAA CTCAGCTGAT AGGGTCAAGA TGGTGCAGGA GGCGGTTCAC 
GGTGTGACGA TGGAGGAGTG CCAAGCCGCA CTCCACAACC ACAACTGGAA TGTCCAGCAA 
GCTGTGCATT ATCTAAAGGT AGAACAGTTG TTCTGTTTGG GCCTGAGGAG CAGATCAGAG 
TGTCTAAAGC TGCTGGAGAT GTGTGACTGG AACCTGGAGG TGGCCAGCAC TCACATGCTG 
GATAACTATG GATCTACAAC ACGACAAAGA CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005458147.1
CDS	1333..4968
Translation	MGETAEYQRLQETTESDYQRLPSDDEEKLGSSMQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKEPPPRPPQPAVLIKKPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_005458090.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005458090.3

ORF Insert Sequence:

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAGGAGCC TCCTCCCCGC CCTCCTCAAC CAGCAGTGTT AATCAAGAAA 
CCTTCCTATG ACCCAGTAAA TGAGGAGGAG GATCTGACTT CTGCGGGACT AAAGAGATTA 
TCACTTCGGA AAACGGGTTC TGTCAAAGGC CTCAAACTAA AACCGGCCGT GTGGGTCTCT 
GCTTCCAAAC AGGGAGGTGG CAGGACTTCA GGCTCAGGCC AGAACACTAA CAGTGAAGTG 
TCCCTCATTG ACTTTGGGGA AGAGTACCCC ATATCCACAC CGTCCCCCTC TCCCGTGGTT 
GAAATTCCAA TTCCTCTATT GGCCAAGTTG GCTTTGGAAG CAGAAAATCT CCTGGACAGG 
ACTCCACCTC AAAGCCCATC CAAATCATTG CCCCGCCCCC TTCACCCTAC GCCAGTAGTG 
GACTGGGATG CGCGGCCATT ACCCCCACCC CCAGCTTATG ACGATGTAGC GCAAGATGAG 
GATGATATGG AGGTGAGCTC CATCAACAGC TCGGAGCAGC AGAATGAGGC AGAGCATAGT 
GATGGCCTTA ATCCAGAAGA AACTCTGTCC TCTGGGCAAA AGGTCAATGA TGAGGCTCTA 
GTCTCCAGAG GTTCAGACAG ACCAGGTCTG GAAGACAACC TGTTTCTCCC CAGCAACCAC 
AGCCAGGGCC TGTCCACCTC TTTTTCACAG TCTGCAGAGA TCTTTCAAGA ACTACAACAA 
GAGTGCATGA GGAGGCTCAA TGTACCAGCA GCAAGTGCTC CTCGTTCGTG CTCGCCATTG 
CAGAGCTCAG TCTTGTGTCC CCAGACTCTT CAAAGTCCCC AAACCCATGA GGGACAACAG 
CAGAGTGTCC TCTCCTGTAA CGAGGACCGA CCCCAGATCC CCCCACGAGT TCCCATCCCC 
CCTCGCCCCA TGAAGAAGGG GGACTACACC TCTGGCCGCT GGTCAAGGGA CCTCTCCCTT 
TCTCCAGTGC CGACTGAAAC CACGGAAAAC GTTTCAGGCG CAGGCCAGGA ACGACCACCT 
CAAATCCCTC CCAGAGACCC TTTGTCTCAG CCTGGATCAA GAACTCCAAG CCCAATGGGT 
CTGGTAGTGG GTTCCCCCCA GCAGAGGCTC TACTCTGTCA GCCCCAACAC AATGCAGGCT 
GCCATTACAA CCTGCCCACC AACACACACC TACAGCTCCT ACCTCTCTAC CTCTCCAGGT 
AAACTGATGC CTACTACACA CAGCTTTGCT TCGGACCCTA AATATGCTGC ACCCAAAGTG 
ATCCAGGCCC AGGTGCAGGG GAAGGACGCT GCTAGCAAGG GCCCCTGTAT CCTGCCCATC 
GTCCGTGACG GGCGTAAAGT CAGTAACACC CACTATTACC TTCTGCCAGA GAGGCCACCG 
TATCTCGATC GATTCGATCG CTTCTTCAGG GAGGCTGAAA GCCTTCCCGC CAACGATGTG 
GAAGAAAAGC ATGTACGGCA AGCCAACACA GCCACGGTCA GACCGATGGT GGTCGGCAGT 
CACACACTGC AGGGGCACAC CCAGGTGCAG GTCCAGCCGG GTGGTGACCT GAAAGCCAAT 
TTCTCCTCCA ATAATAACAG CAATCTGGGT TGGCCACGGT CAGGGATGAA GAGTTCAGTT 
AGTCTTCCTC GCGTCTGTTC AGACGGGCTG ACAGCACCAG CAGTCACTGC TTTCTGTACC 
AGGACAGACG GAGGAGCGAA CTCAGCTGAT AGGGTCAAGA TGGTGCAGGA GGCGGTTCAC 
GGTGTGACGA TGGAGGAGTG CCAAGCCGCA CTCCACAACC ACAACTGGAA TGTCCAGCAA 
GCTGTGCATT ATCTAAAGGT AGAACAGTTG TTCTGTTTGG GCCTGAGGAG CAGATCAGAG 
TGTCTAAAGC TGCTGGAGAT GTGTGACTGG AACCTGGAGG TGGCCAGCAC TCACATGCTG 
GATAACTATG GATCTACAAC ACGACAAAGA CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_005458090.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005458147.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X1, mRNA.

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CloneID	OOr45858
Clone ID Related Accession (Same CDS sequence)	XM_005458090.3 , XM_005458090.4
Accession Version	XM_005458090.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_005458090.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAGGAGCC TCCTCCCCGC CCTCCTCAAC CAGCAGTGTT AATCAAGAAA 
CCTTCCTATG ACCCAGTAAA TGAGGAGGAG GATCTGACTT CTGCGGGACT AAAGAGATTA 
TCACTTCGGA AAACGGGTTC TGTCAAAGGC CTCAAACTAA AACCGGCCGT GTGGGTCTCT 
GCTTCCAAAC AGGGAGGTGG CAGGACTTCA GGCTCAGGCC AGAACACTAA CAGTGAAGTG 
TCCCTCATTG ACTTTGGGGA AGAGTACCCC ATATCCACAC CGTCCCCCTC TCCCGTGGTT 
GAAATTCCAA TTCCTCTATT GGCCAAGTTG GCTTTGGAAG CAGAAAATCT CCTGGACAGG 
ACTCCACCTC AAAGCCCATC CAAATCATTG CCCCGCCCCC TTCACCCTAC GCCAGTAGTG 
GACTGGGATG CGCGGCCATT ACCCCCACCC CCAGCTTATG ACGATGTAGC GCAAGATGAG 
GATGATATGG AGGTGAGCTC CATCAACAGC TCGGAGCAGC AGAATGAGGC AGAGCATAGT 
GATGGCCTTA ATCCAGAAGA AACTCTGTCC TCTGGGCAAA AGGTCAATGA TGAGGCTCTA 
GTCTCCAGAG GTTCAGACAG ACCAGGTCTG GAAGACAACC TGTTTCTCCC CAGCAACCAC 
AGCCAGGGCC TGTCCACCTC TTTTTCACAG TCTGCAGAGA TCTTTCAAGA ACTACAACAA 
GAGTGCATGA GGAGGCTCAA TGTACCAGCA GCAAGTGCTC CTCGTTCGTG CTCGCCATTG 
CAGAGCTCAG TCTTGTGTCC CCAGACTCTT CAAAGTCCCC AAACCCATGA GGGACAACAG 
CAGAGTGTCC TCTCCTGTAA CGAGGACCGA CCCCAGATCC CCCCACGAGT TCCCATCCCC 
CCTCGCCCCA TGAAGAAGGG GGACTACACC TCTGGCCGCT GGTCAAGGGA CCTCTCCCTT 
TCTCCAGTGC CGACTGAAAC CACGGAAAAC GTTTCAGGCG CAGGCCAGGA ACGACCACCT 
CAAATCCCTC CCAGAGACCC TTTGTCTCAG CCTGGATCAA GAACTCCAAG CCCAATGGGT 
CTGGTAGTGG GTTCCCCCCA GCAGAGGCTC TACTCTGTCA GCCCCAACAC AATGCAGGCT 
GCCATTACAA CCTGCCCACC AACACACACC TACAGCTCCT ACCTCTCTAC CTCTCCAGGT 
AAACTGATGC CTACTACACA CAGCTTTGCT TCGGACCCTA AATATGCTGC ACCCAAAGTG 
ATCCAGGCCC AGGTGCAGGG GAAGGACGCT GCTAGCAAGG GCCCCTGTAT CCTGCCCATC 
GTCCGTGACG GGCGTAAAGT CAGTAACACC CACTATTACC TTCTGCCAGA GAGGCCACCG 
TATCTCGATC GATTCGATCG CTTCTTCAGG GAGGCTGAAA GCCTTCCCGC CAACGATGTG 
GAAGAAAAGC ATGTACGGCA AGCCAACACA GCCACGGTCA GACCGATGGT GGTCGGCAGT 
CACACACTGC AGGGGCACAC CCAGGTGCAG GTCCAGCCGG GTGGTGACCT GAAAGCCAAT 
TTCTCCTCCA ATAATAACAG CAATCTGGGT TGGCCACGGT CAGGGATGAA GAGTTCAGTT 
AGTCTTCCTC GCGTCTGTTC AGACGGGCTG ACAGCACCAG CAGTCACTGC TTTCTGTACC 
AGGACAGACG GAGGAGCGAA CTCAGCTGAT AGGGTCAAGA TGGTGCAGGA GGCGGTTCAC 
GGTGTGACGA TGGAGGAGTG CCAAGCCGCA CTCCACAACC ACAACTGGAA TGTCCAGCAA 
GCTGTGCATT ATCTAAAGGT AGAACAGTTG TTCTGTTTGG GCCTGAGGAG CAGATCAGAG 
TGTCTAAAGC TGCTGGAGAT GTGTGACTGG AACCTGGAGG TGGCCAGCAC TCACATGCTG 
GATAACTATG GATCTACAAC ACGACAAAGA CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005458147.1
CDS	1306..4941
Translation	MGETAEYQRLQETTESDYQRLPSDDEEKLGSSMQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKEPPPRPPQPAVLIKKPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_005458090.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005458090.4

ORF Insert Sequence:

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAGGAGCC TCCTCCCCGC CCTCCTCAAC CAGCAGTGTT AATCAAGAAA 
CCTTCCTATG ACCCAGTAAA TGAGGAGGAG GATCTGACTT CTGCGGGACT AAAGAGATTA 
TCACTTCGGA AAACGGGTTC TGTCAAAGGC CTCAAACTAA AACCGGCCGT GTGGGTCTCT 
GCTTCCAAAC AGGGAGGTGG CAGGACTTCA GGCTCAGGCC AGAACACTAA CAGTGAAGTG 
TCCCTCATTG ACTTTGGGGA AGAGTACCCC ATATCCACAC CGTCCCCCTC TCCCGTGGTT 
GAAATTCCAA TTCCTCTATT GGCCAAGTTG GCTTTGGAAG CAGAAAATCT CCTGGACAGG 
ACTCCACCTC AAAGCCCATC CAAATCATTG CCCCGCCCCC TTCACCCTAC GCCAGTAGTG 
GACTGGGATG CGCGGCCATT ACCCCCACCC CCAGCTTATG ACGATGTAGC GCAAGATGAG 
GATGATATGG AGGTGAGCTC CATCAACAGC TCGGAGCAGC AGAATGAGGC AGAGCATAGT 
GATGGCCTTA ATCCAGAAGA AACTCTGTCC TCTGGGCAAA AGGTCAATGA TGAGGCTCTA 
GTCTCCAGAG GTTCAGACAG ACCAGGTCTG GAAGACAACC TGTTTCTCCC CAGCAACCAC 
AGCCAGGGCC TGTCCACCTC TTTTTCACAG TCTGCAGAGA TCTTTCAAGA ACTACAACAA 
GAGTGCATGA GGAGGCTCAA TGTACCAGCA GCAAGTGCTC CTCGTTCGTG CTCGCCATTG 
CAGAGCTCAG TCTTGTGTCC CCAGACTCTT CAAAGTCCCC AAACCCATGA GGGACAACAG 
CAGAGTGTCC TCTCCTGTAA CGAGGACCGA CCCCAGATCC CCCCACGAGT TCCCATCCCC 
CCTCGCCCCA TGAAGAAGGG GGACTACACC TCTGGCCGCT GGTCAAGGGA CCTCTCCCTT 
TCTCCAGTGC CGACTGAAAC CACGGAAAAC GTTTCAGGCG CAGGCCAGGA ACGACCACCT 
CAAATCCCTC CCAGAGACCC TTTGTCTCAG CCTGGATCAA GAACTCCAAG CCCAATGGGT 
CTGGTAGTGG GTTCCCCCCA GCAGAGGCTC TACTCTGTCA GCCCCAACAC AATGCAGGCT 
GCCATTACAA CCTGCCCACC AACACACACC TACAGCTCCT ACCTCTCTAC CTCTCCAGGT 
AAACTGATGC CTACTACACA CAGCTTTGCT TCGGACCCTA AATATGCTGC ACCCAAAGTG 
ATCCAGGCCC AGGTGCAGGG GAAGGACGCT GCTAGCAAGG GCCCCTGTAT CCTGCCCATC 
GTCCGTGACG GGCGTAAAGT CAGTAACACC CACTATTACC TTCTGCCAGA GAGGCCACCG 
TATCTCGATC GATTCGATCG CTTCTTCAGG GAGGCTGAAA GCCTTCCCGC CAACGATGTG 
GAAGAAAAGC ATGTACGGCA AGCCAACACA GCCACGGTCA GACCGATGGT GGTCGGCAGT 
CACACACTGC AGGGGCACAC CCAGGTGCAG GTCCAGCCGG GTGGTGACCT GAAAGCCAAT 
TTCTCCTCCA ATAATAACAG CAATCTGGGT TGGCCACGGT CAGGGATGAA GAGTTCAGTT 
AGTCTTCCTC GCGTCTGTTC AGACGGGCTG ACAGCACCAG CAGTCACTGC TTTCTGTACC 
AGGACAGACG GAGGAGCGAA CTCAGCTGAT AGGGTCAAGA TGGTGCAGGA GGCGGTTCAC 
GGTGTGACGA TGGAGGAGTG CCAAGCCGCA CTCCACAACC ACAACTGGAA TGTCCAGCAA 
GCTGTGCATT ATCTAAAGGT AGAACAGTTG TTCTGTTTGG GCCTGAGGAG CAGATCAGAG 
TGTCTAAAGC TGCTGGAGAT GTGTGACTGG AACCTGGAGG TGGCCAGCAC TCACATGCTG 
GATAACTATG GATCTACAAC ACGACAAAGA CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_019347436.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_019202981.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X4, mRNA.

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CloneID	OOr45859
Clone ID Related Accession (Same CDS sequence)	XM_019347436.1
Accession Version	XM_019347436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATGTG AGGAAGGGAC GGAATGGTTG CTGGAACTGC TGATGGAAGT GCAGCTGCAG 
CAGTACTTCT TACGGATCCG AGATGACCTT AATGTCACGC GGCTGTCGCA CTTTGACTAC 
GTCAAGAATG AAGACCTGGA GAAAATCGGC ATGGGTCGAC CTGGGCAGAG AAGGCTGTGG 
GAGGCCGTGA AAAGGAGGAA AGCCATGTGC AAGCGCAAGT CCTGGATGAG CAAGGTTTTT 
AGTGGTAAAC GTCCAGATGG AGGAGACGTG CCCCAGCAGG GTCAACCGGC TTCCTCGTTT 
CGTAAGCTGT CTCCCACACC ACCTCTGGGC GTCGGGGAGG GAGTCCTAGT CACGCAGCCC 
AGCGGTGGCG CTCTCCTCGA TGGGCAGCAG CAGGGTCTGA CCTGCCTCAT CCCAGAGAAG 
GACTTGACGC TGTTTGAGAA GCTGGGTGAC GGCTCATTTG GGGTCGTGAA GAGGGGAGAG 
TGGTTGACAC CTGCGGGGAA GGTGCTGAAC GTGGCCGTGA AGTGTCTGAA GACAGATGTG 
CTCAGCCAGC CCGACGCTCT GGAGGATTTC ATCTGCGAGG TCAACGTCAT GCACTCGCTG 
GACCATCAAA ACCTGATCCG CCTCTACGGT GTGGTGCTCA CACACCCAAT GAAGATGGTG 
ACTGAACTGG CACCTCTCGG TTCTCTGCTG GAGCGTTTGC GCTGCGTTCG TCCTCAAGGC 
CCAGTGTTGA TCCACACTCT GTGTCAGTAT GCTGTGCAGG TGGCTTGTGG CATGGCTTAT 
CTGGAGCAGA GGAGGTTCAT CCACAGGGAC CTGGCAGCGA GGAACATCCT GCTGGCCGCG 
GCTCACAGAG TGAAGATTGG TGACTTTGGC CTGATGAGGG CGCTGCCTAA CAACCACGAG 
CACTATGTCA TGCAGGAGCA TCGCAAGGTC CCCTTTGCAT GGTGTGCCCC AGAGAGTTTA 
AAGACCAGAA CCTTCTCCCA TGCAACAGAC ACTTGGATGT TTGGAGTCAC CCTCTGGGAG 
ATGTTCACAC ATGGTCAGGA GCCTTGGCTG GGTCTTAATG GTAGTCAGAT TCTCCATAAG 
ATTGATAAAG AGGGTGAACG CCTCCCTAAG CCAGAGGATT GTCCACAGGA TATCTATAAC 
GTCATGCTGC AGTGCTGGGC TCAGAAACCT GATGACAGAC CAACTTTTGT TGCTCTACGC 
GAGTTCCTGC TCGAGTCTAT GCCTACAGAC ATGTGTGCTC TCCAGGACTT TGATGAGCCT 
GACAAACTCC AGATCCAGGT CGATGACATT ATCACCATCA TAGAGGGGAG GGCAGAGAAT 
TATTGGTGGC GAGGTCAAAA TAAGCGCACC CTTAAGGTCG GGCAGTTCCC CAGGAATGTG 
GTGACTTCTG TAGCAGGGTT GTCTGCACAC GACATCAGCC GTCCACTCAA GAACAGCTTC 
ATTCACACAG GACATGGAGA CACCAACCCC CATCGCTGCT GGGGCTTCCC TGACAGGATC 
GATGATTTGT ACCTCGGGAA TCCCATGGAT CCTCCTGACG TTCTGGGCTT GGACCTCAGT 
GCTGCACAGC CCACACAGCT AGCAGGACGA GCTAAAAAGG AGCCTCCTCC CCGCCCTCCT 
CAACCAGCAG TGTTAATCAA GAGTAAGTCT GGTTTCTCTC AGCAGCTCTT CACCCATTGC 
TCCTGCCCTC TCTGTTCCCT CCTAGCTCCA CTCCTCAAAG AACCTTCCTA TGACCCAGTA 
AATGAGGAGG AGGATCTGAC TTCTGCGGGA CTAAAGAGAT TATCACTTCG GAAAACGGGT 
TCTGTCAAAG GCCTCAAACT AAAACCGGCC GTGTGGGTCT CTGCTTCCAA ACAGGGAGGT 
GGCAGGACTT CAGGCTCAGG CCAGAACACT AACAGTGAAG TGTCCCTCAT TGACTTTGGG 
GAAGAGTACC CCATATCCAC ACCGTCCCCC TCTCCCGTGG TTGAAATTCC AATTCCTCTA 
TTGGCCAAGT TGGCTTTGGA AGCAGAAAAT CTCCTGGACA GGACTCCACC TCAAAGCCCA 
TCCAAATCAT TGCCCCGCCC CCTTCACCCT ACGCCAGTAG TGGACTGGGA TGCGCGGCCA 
TTACCCCCAC CCCCAGCTTA TGACGATGTA GCGCAAGATG AGGATGATAT GGAGGTGAGC 
TCCATCAACA GCTCGGAGCA GCAGAATGAG GCAGAGCATA GTGATGGCCT TAATCCAGAA 
GAAACTCTGT CCTCTGGGCA AAAGGTCAAT GATGAGGCTC TAGTCTCCAG AGGTTCAGAC 
AGACCAGGTC TGGAAGACAA CCTGTTTCTC CCCAGCAACC ACAGCCAGGG CCTGTCCACC 
TCTTTTTCAC AGTCTGCAGA GATCTTTCAA GAACTACAAC AAGAGTGCAT GAGGAGGCTC 
AATGTACCAG CAGCAAGTGC TCCTCGTTCG TGCTCGCCAT TGCAGAGCTC AGTCTTGTGT 
CCCCAGACTC TTCAAAGTCC CCAAACCCAT GAGGGACAAC AGCAGAGTGT CCTCTCCTGT 
AACGAGGACC GACCCCAGAT CCCCCCACGA GTTCCCATCC CCCCTCGCCC CATGAAGAAG 
GGGGACTACA CCTCTGGCCG CTGGTCAAGG GACCTCTCCC TTTCTCCAGT GCCGACTGAA 
ACCACGGAAA ACGTTTCAGG CGCAGGCCAG GAACGACCAC CTCAAATCCC TCCCAGAGAC 
CCTTTGTCTC AGCCTGGATC AAGAACTCCA AGCCCAATGG GTCTGGTAGT GGGTTCCCCC 
CAGCAGAGGC TCTACTCTGT CAGCCCCAAC ACAATGCAGG CTGCCATTAC AACCTGCCCA 
CCAACACACA CCTACAGCTC CTACCTCTCT ACCTCTCCAG GTAAACTGAT GCCTACTACA 
CACAGCTTTG CTTCGGACCC TAAATATGCT GCACCCAAAG TGATCCAGGC CCAGGTGCAG 
GGGAAGGACG CTGCTAGCAA GGGCCCCTGT ATCCTGCCCA TCGTCCGTGA CGGGCGTAAA 
GTCAGTAACA CCCACTATTA CCTTCTGCCA GAGAGGCCAC CGTATCTCGA TCGATTCGAT 
CGCTTCTTCA GGGAGGCTGA AAGCCTTCCC GCCAACGATG TGGAAGAAAA GCATGTACGG 
CAAGCCAACA CAGCCACGGT CAGACCGATG GTGGTCGGCA GTCACACACT GCAGGGGCAC 
ACCCAGGTGC AGGTCCAGCC GGGTGGTGAC CTGAAAGCCA ATTTCTCCTC CAATAATAAC 
AGCAATCTGG GTTGGCCACG GTCAGGGATG AAGAGTTCAG TTAGTCTTCC TCGCGTCTGT 
TCAGACGGGC TGACAGCACC AGCAGTCACT GCTTTCTGTA CCAGGACAGA CGGAGGAGCG 
AACTCAGCTG ATAGGGTCAA GATGGTGCAG GAGGCGGTTC ACGGTGTGAC GATGGAGGAG 
TGCCAAGCCG CACTCCACAA CCACAACTGG AATGTCCAGC AAGCTGTGCA TTATCTAAAG 
GTAGAACAGT TGTTCTGTTT GGGCCTGAGG AGCAGATCAG AGTGTCTAAA GCTGCTGGAG 
ATGTGTGACT GGAACCTGGA GGTGGCCAGC ACTCACATGC TGGATAACTA TGGATCTACA 
ACACGACAAA GACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019202981.1
CDS	513..4130
Translation	MQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKEPPPRPPQPAVLIKSKSGFSQQLFTHCSCPLCSLLAPLLKEPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_019347436.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019347436.1

ORF Insert Sequence:

ATGCAATGTG AGGAAGGGAC GGAATGGTTG CTGGAACTGC TGATGGAAGT GCAGCTGCAG 
CAGTACTTCT TACGGATCCG AGATGACCTT AATGTCACGC GGCTGTCGCA CTTTGACTAC 
GTCAAGAATG AAGACCTGGA GAAAATCGGC ATGGGTCGAC CTGGGCAGAG AAGGCTGTGG 
GAGGCCGTGA AAAGGAGGAA AGCCATGTGC AAGCGCAAGT CCTGGATGAG CAAGGTTTTT 
AGTGGTAAAC GTCCAGATGG AGGAGACGTG CCCCAGCAGG GTCAACCGGC TTCCTCGTTT 
CGTAAGCTGT CTCCCACACC ACCTCTGGGC GTCGGGGAGG GAGTCCTAGT CACGCAGCCC 
AGCGGTGGCG CTCTCCTCGA TGGGCAGCAG CAGGGTCTGA CCTGCCTCAT CCCAGAGAAG 
GACTTGACGC TGTTTGAGAA GCTGGGTGAC GGCTCATTTG GGGTCGTGAA GAGGGGAGAG 
TGGTTGACAC CTGCGGGGAA GGTGCTGAAC GTGGCCGTGA AGTGTCTGAA GACAGATGTG 
CTCAGCCAGC CCGACGCTCT GGAGGATTTC ATCTGCGAGG TCAACGTCAT GCACTCGCTG 
GACCATCAAA ACCTGATCCG CCTCTACGGT GTGGTGCTCA CACACCCAAT GAAGATGGTG 
ACTGAACTGG CACCTCTCGG TTCTCTGCTG GAGCGTTTGC GCTGCGTTCG TCCTCAAGGC 
CCAGTGTTGA TCCACACTCT GTGTCAGTAT GCTGTGCAGG TGGCTTGTGG CATGGCTTAT 
CTGGAGCAGA GGAGGTTCAT CCACAGGGAC CTGGCAGCGA GGAACATCCT GCTGGCCGCG 
GCTCACAGAG TGAAGATTGG TGACTTTGGC CTGATGAGGG CGCTGCCTAA CAACCACGAG 
CACTATGTCA TGCAGGAGCA TCGCAAGGTC CCCTTTGCAT GGTGTGCCCC AGAGAGTTTA 
AAGACCAGAA CCTTCTCCCA TGCAACAGAC ACTTGGATGT TTGGAGTCAC CCTCTGGGAG 
ATGTTCACAC ATGGTCAGGA GCCTTGGCTG GGTCTTAATG GTAGTCAGAT TCTCCATAAG 
ATTGATAAAG AGGGTGAACG CCTCCCTAAG CCAGAGGATT GTCCACAGGA TATCTATAAC 
GTCATGCTGC AGTGCTGGGC TCAGAAACCT GATGACAGAC CAACTTTTGT TGCTCTACGC 
GAGTTCCTGC TCGAGTCTAT GCCTACAGAC ATGTGTGCTC TCCAGGACTT TGATGAGCCT 
GACAAACTCC AGATCCAGGT CGATGACATT ATCACCATCA TAGAGGGGAG GGCAGAGAAT 
TATTGGTGGC GAGGTCAAAA TAAGCGCACC CTTAAGGTCG GGCAGTTCCC CAGGAATGTG 
GTGACTTCTG TAGCAGGGTT GTCTGCACAC GACATCAGCC GTCCACTCAA GAACAGCTTC 
ATTCACACAG GACATGGAGA CACCAACCCC CATCGCTGCT GGGGCTTCCC TGACAGGATC 
GATGATTTGT ACCTCGGGAA TCCCATGGAT CCTCCTGACG TTCTGGGCTT GGACCTCAGT 
GCTGCACAGC CCACACAGCT AGCAGGACGA GCTAAAAAGG AGCCTCCTCC CCGCCCTCCT 
CAACCAGCAG TGTTAATCAA GAGTAAGTCT GGTTTCTCTC AGCAGCTCTT CACCCATTGC 
TCCTGCCCTC TCTGTTCCCT CCTAGCTCCA CTCCTCAAAG AACCTTCCTA TGACCCAGTA 
AATGAGGAGG AGGATCTGAC TTCTGCGGGA CTAAAGAGAT TATCACTTCG GAAAACGGGT 
TCTGTCAAAG GCCTCAAACT AAAACCGGCC GTGTGGGTCT CTGCTTCCAA ACAGGGAGGT 
GGCAGGACTT CAGGCTCAGG CCAGAACACT AACAGTGAAG TGTCCCTCAT TGACTTTGGG 
GAAGAGTACC CCATATCCAC ACCGTCCCCC TCTCCCGTGG TTGAAATTCC AATTCCTCTA 
TTGGCCAAGT TGGCTTTGGA AGCAGAAAAT CTCCTGGACA GGACTCCACC TCAAAGCCCA 
TCCAAATCAT TGCCCCGCCC CCTTCACCCT ACGCCAGTAG TGGACTGGGA TGCGCGGCCA 
TTACCCCCAC CCCCAGCTTA TGACGATGTA GCGCAAGATG AGGATGATAT GGAGGTGAGC 
TCCATCAACA GCTCGGAGCA GCAGAATGAG GCAGAGCATA GTGATGGCCT TAATCCAGAA 
GAAACTCTGT CCTCTGGGCA AAAGGTCAAT GATGAGGCTC TAGTCTCCAG AGGTTCAGAC 
AGACCAGGTC TGGAAGACAA CCTGTTTCTC CCCAGCAACC ACAGCCAGGG CCTGTCCACC 
TCTTTTTCAC AGTCTGCAGA GATCTTTCAA GAACTACAAC AAGAGTGCAT GAGGAGGCTC 
AATGTACCAG CAGCAAGTGC TCCTCGTTCG TGCTCGCCAT TGCAGAGCTC AGTCTTGTGT 
CCCCAGACTC TTCAAAGTCC CCAAACCCAT GAGGGACAAC AGCAGAGTGT CCTCTCCTGT 
AACGAGGACC GACCCCAGAT CCCCCCACGA GTTCCCATCC CCCCTCGCCC CATGAAGAAG 
GGGGACTACA CCTCTGGCCG CTGGTCAAGG GACCTCTCCC TTTCTCCAGT GCCGACTGAA 
ACCACGGAAA ACGTTTCAGG CGCAGGCCAG GAACGACCAC CTCAAATCCC TCCCAGAGAC 
CCTTTGTCTC AGCCTGGATC AAGAACTCCA AGCCCAATGG GTCTGGTAGT GGGTTCCCCC 
CAGCAGAGGC TCTACTCTGT CAGCCCCAAC ACAATGCAGG CTGCCATTAC AACCTGCCCA 
CCAACACACA CCTACAGCTC CTACCTCTCT ACCTCTCCAG GTAAACTGAT GCCTACTACA 
CACAGCTTTG CTTCGGACCC TAAATATGCT GCACCCAAAG TGATCCAGGC CCAGGTGCAG 
GGGAAGGACG CTGCTAGCAA GGGCCCCTGT ATCCTGCCCA TCGTCCGTGA CGGGCGTAAA 
GTCAGTAACA CCCACTATTA CCTTCTGCCA GAGAGGCCAC CGTATCTCGA TCGATTCGAT 
CGCTTCTTCA GGGAGGCTGA AAGCCTTCCC GCCAACGATG TGGAAGAAAA GCATGTACGG 
CAAGCCAACA CAGCCACGGT CAGACCGATG GTGGTCGGCA GTCACACACT GCAGGGGCAC 
ACCCAGGTGC AGGTCCAGCC GGGTGGTGAC CTGAAAGCCA ATTTCTCCTC CAATAATAAC 
AGCAATCTGG GTTGGCCACG GTCAGGGATG AAGAGTTCAG TTAGTCTTCC TCGCGTCTGT 
TCAGACGGGC TGACAGCACC AGCAGTCACT GCTTTCTGTA CCAGGACAGA CGGAGGAGCG 
AACTCAGCTG ATAGGGTCAA GATGGTGCAG GAGGCGGTTC ACGGTGTGAC GATGGAGGAG 
TGCCAAGCCG CACTCCACAA CCACAACTGG AATGTCCAGC AAGCTGTGCA TTATCTAAAG 
GTAGAACAGT TGTTCTGTTT GGGCCTGAGG AGCAGATCAG AGTGTCTAAA GCTGCTGGAG 
ATGTGTGACT GGAACCTGGA GGTGGCCAGC ACTCACATGC TGGATAACTA TGGATCTACA 
ACACGACAAA GACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_005458091.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005458148.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOr45860
Clone ID Related Accession (Same CDS sequence)	XM_005458091.3 , XM_005458091.4
Accession Version	XM_005458091.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_005458091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAACCTTC CTATGACCCA GTAAATGAGG AGGAGGATCT GACTTCTGCG 
GGACTAAAGA GATTATCACT TCGGAAAACG GGTTCTGTCA AAGGCCTCAA ACTAAAACCG 
GCCGTGTGGG TCTCTGCTTC CAAACAGGGA GGTGGCAGGA CTTCAGGCTC AGGCCAGAAC 
ACTAACAGTG AAGTGTCCCT CATTGACTTT GGGGAAGAGT ACCCCATATC CACACCGTCC 
CCCTCTCCCG TGGTTGAAAT TCCAATTCCT CTATTGGCCA AGTTGGCTTT GGAAGCAGAA 
AATCTCCTGG ACAGGACTCC ACCTCAAAGC CCATCCAAAT CATTGCCCCG CCCCCTTCAC 
CCTACGCCAG TAGTGGACTG GGATGCGCGG CCATTACCCC CACCCCCAGC TTATGACGAT 
GTAGCGCAAG ATGAGGATGA TATGGAGGTG AGCTCCATCA ACAGCTCGGA GCAGCAGAAT 
GAGGCAGAGC ATAGTGATGG CCTTAATCCA GAAGAAACTC TGTCCTCTGG GCAAAAGGTC 
AATGATGAGG CTCTAGTCTC CAGAGGTTCA GACAGACCAG GTCTGGAAGA CAACCTGTTT 
CTCCCCAGCA ACCACAGCCA GGGCCTGTCC ACCTCTTTTT CACAGTCTGC AGAGATCTTT 
CAAGAACTAC AACAAGAGTG CATGAGGAGG CTCAATGTAC CAGCAGCAAG TGCTCCTCGT 
TCGTGCTCGC CATTGCAGAG CTCAGTCTTG TGTCCCCAGA CTCTTCAAAG TCCCCAAACC 
CATGAGGGAC AACAGCAGAG TGTCCTCTCC TGTAACGAGG ACCGACCCCA GATCCCCCCA 
CGAGTTCCCA TCCCCCCTCG CCCCATGAAG AAGGGGGACT ACACCTCTGG CCGCTGGTCA 
AGGGACCTCT CCCTTTCTCC AGTGCCGACT GAAACCACGG AAAACGTTTC AGGCGCAGGC 
CAGGAACGAC CACCTCAAAT CCCTCCCAGA GACCCTTTGT CTCAGCCTGG ATCAAGAACT 
CCAAGCCCAA TGGGTCTGGT AGTGGGTTCC CCCCAGCAGA GGCTCTACTC TGTCAGCCCC 
AACACAATGC AGGCTGCCAT TACAACCTGC CCACCAACAC ACACCTACAG CTCCTACCTC 
TCTACCTCTC CAGGTAAACT GATGCCTACT ACACACAGCT TTGCTTCGGA CCCTAAATAT 
GCTGCACCCA AAGTGATCCA GGCCCAGGTG CAGGGGAAGG ACGCTGCTAG CAAGGGCCCC 
TGTATCCTGC CCATCGTCCG TGACGGGCGT AAAGTCAGTA ACACCCACTA TTACCTTCTG 
CCAGAGAGGC CACCGTATCT CGATCGATTC GATCGCTTCT TCAGGGAGGC TGAAAGCCTT 
CCCGCCAACG ATGTGGAAGA AAAGCATGTA CGGCAAGCCA ACACAGCCAC GGTCAGACCG 
ATGGTGGTCG GCAGTCACAC ACTGCAGGGG CACACCCAGG TGCAGGTCCA GCCGGGTGGT 
GACCTGAAAG CCAATTTCTC CTCCAATAAT AACAGCAATC TGGGTTGGCC ACGGTCAGGG 
ATGAAGAGTT CAGTTAGTCT TCCTCGCGTC TGTTCAGACG GGCTGACAGC ACCAGCAGTC 
ACTGCTTTCT GTACCAGGAC AGACGGAGGA GCGAACTCAG CTGATAGGGT CAAGATGGTG 
CAGGAGGCGG TTCACGGTGT GACGATGGAG GAGTGCCAAG CCGCACTCCA CAACCACAAC 
TGGAATGTCC AGCAAGCTGT GCATTATCTA AAGGTAGAAC AGTTGTTCTG TTTGGGCCTG 
AGGAGCAGAT CAGAGTGTCT AAAGCTGCTG GAGATGTGTG ACTGGAACCT GGAGGTGGCC 
AGCACTCACA TGCTGGATAA CTATGGATCT ACAACACGAC AAAGACGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005458148.1
CDS	1332..4922
Translation	MGETAEYQRLQETTESDYQRLPSDDEEKLGSSMQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_005458091.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005458091.3

ORF Insert Sequence:

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAACCTTC CTATGACCCA GTAAATGAGG AGGAGGATCT GACTTCTGCG 
GGACTAAAGA GATTATCACT TCGGAAAACG GGTTCTGTCA AAGGCCTCAA ACTAAAACCG 
GCCGTGTGGG TCTCTGCTTC CAAACAGGGA GGTGGCAGGA CTTCAGGCTC AGGCCAGAAC 
ACTAACAGTG AAGTGTCCCT CATTGACTTT GGGGAAGAGT ACCCCATATC CACACCGTCC 
CCCTCTCCCG TGGTTGAAAT TCCAATTCCT CTATTGGCCA AGTTGGCTTT GGAAGCAGAA 
AATCTCCTGG ACAGGACTCC ACCTCAAAGC CCATCCAAAT CATTGCCCCG CCCCCTTCAC 
CCTACGCCAG TAGTGGACTG GGATGCGCGG CCATTACCCC CACCCCCAGC TTATGACGAT 
GTAGCGCAAG ATGAGGATGA TATGGAGGTG AGCTCCATCA ACAGCTCGGA GCAGCAGAAT 
GAGGCAGAGC ATAGTGATGG CCTTAATCCA GAAGAAACTC TGTCCTCTGG GCAAAAGGTC 
AATGATGAGG CTCTAGTCTC CAGAGGTTCA GACAGACCAG GTCTGGAAGA CAACCTGTTT 
CTCCCCAGCA ACCACAGCCA GGGCCTGTCC ACCTCTTTTT CACAGTCTGC AGAGATCTTT 
CAAGAACTAC AACAAGAGTG CATGAGGAGG CTCAATGTAC CAGCAGCAAG TGCTCCTCGT 
TCGTGCTCGC CATTGCAGAG CTCAGTCTTG TGTCCCCAGA CTCTTCAAAG TCCCCAAACC 
CATGAGGGAC AACAGCAGAG TGTCCTCTCC TGTAACGAGG ACCGACCCCA GATCCCCCCA 
CGAGTTCCCA TCCCCCCTCG CCCCATGAAG AAGGGGGACT ACACCTCTGG CCGCTGGTCA 
AGGGACCTCT CCCTTTCTCC AGTGCCGACT GAAACCACGG AAAACGTTTC AGGCGCAGGC 
CAGGAACGAC CACCTCAAAT CCCTCCCAGA GACCCTTTGT CTCAGCCTGG ATCAAGAACT 
CCAAGCCCAA TGGGTCTGGT AGTGGGTTCC CCCCAGCAGA GGCTCTACTC TGTCAGCCCC 
AACACAATGC AGGCTGCCAT TACAACCTGC CCACCAACAC ACACCTACAG CTCCTACCTC 
TCTACCTCTC CAGGTAAACT GATGCCTACT ACACACAGCT TTGCTTCGGA CCCTAAATAT 
GCTGCACCCA AAGTGATCCA GGCCCAGGTG CAGGGGAAGG ACGCTGCTAG CAAGGGCCCC 
TGTATCCTGC CCATCGTCCG TGACGGGCGT AAAGTCAGTA ACACCCACTA TTACCTTCTG 
CCAGAGAGGC CACCGTATCT CGATCGATTC GATCGCTTCT TCAGGGAGGC TGAAAGCCTT 
CCCGCCAACG ATGTGGAAGA AAAGCATGTA CGGCAAGCCA ACACAGCCAC GGTCAGACCG 
ATGGTGGTCG GCAGTCACAC ACTGCAGGGG CACACCCAGG TGCAGGTCCA GCCGGGTGGT 
GACCTGAAAG CCAATTTCTC CTCCAATAAT AACAGCAATC TGGGTTGGCC ACGGTCAGGG 
ATGAAGAGTT CAGTTAGTCT TCCTCGCGTC TGTTCAGACG GGCTGACAGC ACCAGCAGTC 
ACTGCTTTCT GTACCAGGAC AGACGGAGGA GCGAACTCAG CTGATAGGGT CAAGATGGTG 
CAGGAGGCGG TTCACGGTGT GACGATGGAG GAGTGCCAAG CCGCACTCCA CAACCACAAC 
TGGAATGTCC AGCAAGCTGT GCATTATCTA AAGGTAGAAC AGTTGTTCTG TTTGGGCCTG 
AGGAGCAGAT CAGAGTGTCT AAAGCTGCTG GAGATGTGTG ACTGGAACCT GGAGGTGGCC 
AGCACTCACA TGCTGGATAA CTATGGATCT ACAACACGAC AAAGACGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tnk2 ( XM_005458091.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005458148.1 Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X3, mRNA.

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CloneID	OOr45860
Clone ID Related Accession (Same CDS sequence)	XM_005458091.3 , XM_005458091.4
Accession Version	XM_005458091.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	activated CDC42 kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031982.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_005458091.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAACCTTC CTATGACCCA GTAAATGAGG AGGAGGATCT GACTTCTGCG 
GGACTAAAGA GATTATCACT TCGGAAAACG GGTTCTGTCA AAGGCCTCAA ACTAAAACCG 
GCCGTGTGGG TCTCTGCTTC CAAACAGGGA GGTGGCAGGA CTTCAGGCTC AGGCCAGAAC 
ACTAACAGTG AAGTGTCCCT CATTGACTTT GGGGAAGAGT ACCCCATATC CACACCGTCC 
CCCTCTCCCG TGGTTGAAAT TCCAATTCCT CTATTGGCCA AGTTGGCTTT GGAAGCAGAA 
AATCTCCTGG ACAGGACTCC ACCTCAAAGC CCATCCAAAT CATTGCCCCG CCCCCTTCAC 
CCTACGCCAG TAGTGGACTG GGATGCGCGG CCATTACCCC CACCCCCAGC TTATGACGAT 
GTAGCGCAAG ATGAGGATGA TATGGAGGTG AGCTCCATCA ACAGCTCGGA GCAGCAGAAT 
GAGGCAGAGC ATAGTGATGG CCTTAATCCA GAAGAAACTC TGTCCTCTGG GCAAAAGGTC 
AATGATGAGG CTCTAGTCTC CAGAGGTTCA GACAGACCAG GTCTGGAAGA CAACCTGTTT 
CTCCCCAGCA ACCACAGCCA GGGCCTGTCC ACCTCTTTTT CACAGTCTGC AGAGATCTTT 
CAAGAACTAC AACAAGAGTG CATGAGGAGG CTCAATGTAC CAGCAGCAAG TGCTCCTCGT 
TCGTGCTCGC CATTGCAGAG CTCAGTCTTG TGTCCCCAGA CTCTTCAAAG TCCCCAAACC 
CATGAGGGAC AACAGCAGAG TGTCCTCTCC TGTAACGAGG ACCGACCCCA GATCCCCCCA 
CGAGTTCCCA TCCCCCCTCG CCCCATGAAG AAGGGGGACT ACACCTCTGG CCGCTGGTCA 
AGGGACCTCT CCCTTTCTCC AGTGCCGACT GAAACCACGG AAAACGTTTC AGGCGCAGGC 
CAGGAACGAC CACCTCAAAT CCCTCCCAGA GACCCTTTGT CTCAGCCTGG ATCAAGAACT 
CCAAGCCCAA TGGGTCTGGT AGTGGGTTCC CCCCAGCAGA GGCTCTACTC TGTCAGCCCC 
AACACAATGC AGGCTGCCAT TACAACCTGC CCACCAACAC ACACCTACAG CTCCTACCTC 
TCTACCTCTC CAGGTAAACT GATGCCTACT ACACACAGCT TTGCTTCGGA CCCTAAATAT 
GCTGCACCCA AAGTGATCCA GGCCCAGGTG CAGGGGAAGG ACGCTGCTAG CAAGGGCCCC 
TGTATCCTGC CCATCGTCCG TGACGGGCGT AAAGTCAGTA ACACCCACTA TTACCTTCTG 
CCAGAGAGGC CACCGTATCT CGATCGATTC GATCGCTTCT TCAGGGAGGC TGAAAGCCTT 
CCCGCCAACG ATGTGGAAGA AAAGCATGTA CGGCAAGCCA ACACAGCCAC GGTCAGACCG 
ATGGTGGTCG GCAGTCACAC ACTGCAGGGG CACACCCAGG TGCAGGTCCA GCCGGGTGGT 
GACCTGAAAG CCAATTTCTC CTCCAATAAT AACAGCAATC TGGGTTGGCC ACGGTCAGGG 
ATGAAGAGTT CAGTTAGTCT TCCTCGCGTC TGTTCAGACG GGCTGACAGC ACCAGCAGTC 
ACTGCTTTCT GTACCAGGAC AGACGGAGGA GCGAACTCAG CTGATAGGGT CAAGATGGTG 
CAGGAGGCGG TTCACGGTGT GACGATGGAG GAGTGCCAAG CCGCACTCCA CAACCACAAC 
TGGAATGTCC AGCAAGCTGT GCATTATCTA AAGGTAGAAC AGTTGTTCTG TTTGGGCCTG 
AGGAGCAGAT CAGAGTGTCT AAAGCTGCTG GAGATGTGTG ACTGGAACCT GGAGGTGGCC 
AGCACTCACA TGCTGGATAA CTATGGATCT ACAACACGAC AAAGACGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005458148.1
CDS	1305..4895
Translation	MGETAEYQRLQETTESDYQRLPSDDEEKLGSSMQCEEGTEWLLELLMEVQLQQYFLRIRDDLNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRPDGGDVPQQGQPASSFRKLSPTPPLGVGEGVLVTQPSGGALLDGQQQGLTCLIPEKDLTLFEKLGDGSFGVVKRGEWLTPAGKVLNVAVKCLKTDVLSQPDALEDFICEVNVMHSLDHQNLIRLYGVVLTHPMKMVTELAPLGSLLERLRCVRPQGPVLIHTLCQYAVQVACGMAYLEQRRFIHRDLAARNILLAAAHRVKIGDFGLMRALPNNHEHYVMQEHRKVPFAWCAPESLKTRTFSHATDTWMFGVTLWEMFTHGQEPWLGLNGSQILHKIDKEGERLPKPEDCPQDIYNVMLQCWAQKPDDRPTFVALREFLLESMPTDMCALQDFDEPDKLQIQVDDIITIIEGRAENYWWRGQNKRTLKVGQFPRNVVTSVAGLSAHDISRPLKNSFIHTGHGDTNPHRCWGFPDRIDDLYLGNPMDPPDVLGLDLSAAQPTQLAGRAKKPSYDPVNEEEDLTSAGLKRLSLRKTGSVKGLKLKPAVWVSASKQGGGRTSGSGQNTNSEVSLIDFGEEYPISTPSPSPVVEIPIPLLAKLALEAENLLDRTPPQSPSKSLPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDMEVSSINSSEQQNEAEHSDGLNPEETLSSGQKVNDEALVSRGSDRPGLEDNLFLPSNHSQGLSTSFSQSAEIFQELQQECMRRLNVPAASAPRSCSPLQSSVLCPQTLQSPQTHEGQQQSVLSCNEDRPQIPPRVPIPPRPMKKGDYTSGRWSRDLSLSPVPTETTENVSGAGQERPPQIPPRDPLSQPGSRTPSPMGLVVGSPQQRLYSVSPNTMQAAITTCPPTHTYSSYLSTSPGKLMPTTHSFASDPKYAAPKVIQAQVQGKDAASKGPCILPIVRDGRKVSNTHYYLLPERPPYLDRFDRFFREAESLPANDVEEKHVRQANTATVRPMVVGSHTLQGHTQVQVQPGGDLKANFSSNNNSNLGWPRSGMKSSVSLPRVCSDGLTAPAVTAFCTRTDGGANSADRVKMVQEAVHGVTMEECQAALHNHNWNVQQAVHYLKVEQLFCLGLRSRSECLKLLEMCDWNLEVASTHMLDNYGSTTRQRR

Target ORF information:

RefSeq Version	XM_005458091.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus tyrosine kinase non receptor 2 (tnk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005458091.4

ORF Insert Sequence:

ATGGGGGAGA CGGCAGAATA CCAGAGACTG CAGGAGACGA CAGAGTCGGA TTACCAAAGG 
CTGCCTAGCG ATGATGAAGA GAAACTGGGC AGCAGCATGC AATGTGAGGA AGGGACGGAA 
TGGTTGCTGG AACTGCTGAT GGAAGTGCAG CTGCAGCAGT ACTTCTTACG GATCCGAGAT 
GACCTTAATG TCACGCGGCT GTCGCACTTT GACTACGTCA AGAATGAAGA CCTGGAGAAA 
ATCGGCATGG GTCGACCTGG GCAGAGAAGG CTGTGGGAGG CCGTGAAAAG GAGGAAAGCC 
ATGTGCAAGC GCAAGTCCTG GATGAGCAAG GTTTTTAGTG GTAAACGTCC AGATGGAGGA 
GACGTGCCCC AGCAGGGTCA ACCGGCTTCC TCGTTTCGTA AGCTGTCTCC CACACCACCT 
CTGGGCGTCG GGGAGGGAGT CCTAGTCACG CAGCCCAGCG GTGGCGCTCT CCTCGATGGG 
CAGCAGCAGG GTCTGACCTG CCTCATCCCA GAGAAGGACT TGACGCTGTT TGAGAAGCTG 
GGTGACGGCT CATTTGGGGT CGTGAAGAGG GGAGAGTGGT TGACACCTGC GGGGAAGGTG 
CTGAACGTGG CCGTGAAGTG TCTGAAGACA GATGTGCTCA GCCAGCCCGA CGCTCTGGAG 
GATTTCATCT GCGAGGTCAA CGTCATGCAC TCGCTGGACC ATCAAAACCT GATCCGCCTC 
TACGGTGTGG TGCTCACACA CCCAATGAAG ATGGTGACTG AACTGGCACC TCTCGGTTCT 
CTGCTGGAGC GTTTGCGCTG CGTTCGTCCT CAAGGCCCAG TGTTGATCCA CACTCTGTGT 
CAGTATGCTG TGCAGGTGGC TTGTGGCATG GCTTATCTGG AGCAGAGGAG GTTCATCCAC 
AGGGACCTGG CAGCGAGGAA CATCCTGCTG GCCGCGGCTC ACAGAGTGAA GATTGGTGAC 
TTTGGCCTGA TGAGGGCGCT GCCTAACAAC CACGAGCACT ATGTCATGCA GGAGCATCGC 
AAGGTCCCCT TTGCATGGTG TGCCCCAGAG AGTTTAAAGA CCAGAACCTT CTCCCATGCA 
ACAGACACTT GGATGTTTGG AGTCACCCTC TGGGAGATGT TCACACATGG TCAGGAGCCT 
TGGCTGGGTC TTAATGGTAG TCAGATTCTC CATAAGATTG ATAAAGAGGG TGAACGCCTC 
CCTAAGCCAG AGGATTGTCC ACAGGATATC TATAACGTCA TGCTGCAGTG CTGGGCTCAG 
AAACCTGATG ACAGACCAAC TTTTGTTGCT CTACGCGAGT TCCTGCTCGA GTCTATGCCT 
ACAGACATGT GTGCTCTCCA GGACTTTGAT GAGCCTGACA AACTCCAGAT CCAGGTCGAT 
GACATTATCA CCATCATAGA GGGGAGGGCA GAGAATTATT GGTGGCGAGG TCAAAATAAG 
CGCACCCTTA AGGTCGGGCA GTTCCCCAGG AATGTGGTGA CTTCTGTAGC AGGGTTGTCT 
GCACACGACA TCAGCCGTCC ACTCAAGAAC AGCTTCATTC ACACAGGACA TGGAGACACC 
AACCCCCATC GCTGCTGGGG CTTCCCTGAC AGGATCGATG ATTTGTACCT CGGGAATCCC 
ATGGATCCTC CTGACGTTCT GGGCTTGGAC CTCAGTGCTG CACAGCCCAC ACAGCTAGCA 
GGACGAGCTA AAAAACCTTC CTATGACCCA GTAAATGAGG AGGAGGATCT GACTTCTGCG 
GGACTAAAGA GATTATCACT TCGGAAAACG GGTTCTGTCA AAGGCCTCAA ACTAAAACCG 
GCCGTGTGGG TCTCTGCTTC CAAACAGGGA GGTGGCAGGA CTTCAGGCTC AGGCCAGAAC 
ACTAACAGTG AAGTGTCCCT CATTGACTTT GGGGAAGAGT ACCCCATATC CACACCGTCC 
CCCTCTCCCG TGGTTGAAAT TCCAATTCCT CTATTGGCCA AGTTGGCTTT GGAAGCAGAA 
AATCTCCTGG ACAGGACTCC ACCTCAAAGC CCATCCAAAT CATTGCCCCG CCCCCTTCAC 
CCTACGCCAG TAGTGGACTG GGATGCGCGG CCATTACCCC CACCCCCAGC TTATGACGAT 
GTAGCGCAAG ATGAGGATGA TATGGAGGTG AGCTCCATCA ACAGCTCGGA GCAGCAGAAT 
GAGGCAGAGC ATAGTGATGG CCTTAATCCA GAAGAAACTC TGTCCTCTGG GCAAAAGGTC 
AATGATGAGG CTCTAGTCTC CAGAGGTTCA GACAGACCAG GTCTGGAAGA CAACCTGTTT 
CTCCCCAGCA ACCACAGCCA GGGCCTGTCC ACCTCTTTTT CACAGTCTGC AGAGATCTTT 
CAAGAACTAC AACAAGAGTG CATGAGGAGG CTCAATGTAC CAGCAGCAAG TGCTCCTCGT 
TCGTGCTCGC CATTGCAGAG CTCAGTCTTG TGTCCCCAGA CTCTTCAAAG TCCCCAAACC 
CATGAGGGAC AACAGCAGAG TGTCCTCTCC TGTAACGAGG ACCGACCCCA GATCCCCCCA 
CGAGTTCCCA TCCCCCCTCG CCCCATGAAG AAGGGGGACT ACACCTCTGG CCGCTGGTCA 
AGGGACCTCT CCCTTTCTCC AGTGCCGACT GAAACCACGG AAAACGTTTC AGGCGCAGGC 
CAGGAACGAC CACCTCAAAT CCCTCCCAGA GACCCTTTGT CTCAGCCTGG ATCAAGAACT 
CCAAGCCCAA TGGGTCTGGT AGTGGGTTCC CCCCAGCAGA GGCTCTACTC TGTCAGCCCC 
AACACAATGC AGGCTGCCAT TACAACCTGC CCACCAACAC ACACCTACAG CTCCTACCTC 
TCTACCTCTC CAGGTAAACT GATGCCTACT ACACACAGCT TTGCTTCGGA CCCTAAATAT 
GCTGCACCCA AAGTGATCCA GGCCCAGGTG CAGGGGAAGG ACGCTGCTAG CAAGGGCCCC 
TGTATCCTGC CCATCGTCCG TGACGGGCGT AAAGTCAGTA ACACCCACTA TTACCTTCTG 
CCAGAGAGGC CACCGTATCT CGATCGATTC GATCGCTTCT TCAGGGAGGC TGAAAGCCTT 
CCCGCCAACG ATGTGGAAGA AAAGCATGTA CGGCAAGCCA ACACAGCCAC GGTCAGACCG 
ATGGTGGTCG GCAGTCACAC ACTGCAGGGG CACACCCAGG TGCAGGTCCA GCCGGGTGGT 
GACCTGAAAG CCAATTTCTC CTCCAATAAT AACAGCAATC TGGGTTGGCC ACGGTCAGGG 
ATGAAGAGTT CAGTTAGTCT TCCTCGCGTC TGTTCAGACG GGCTGACAGC ACCAGCAGTC 
ACTGCTTTCT GTACCAGGAC AGACGGAGGA GCGAACTCAG CTGATAGGGT CAAGATGGTG 
CAGGAGGCGG TTCACGGTGT GACGATGGAG GAGTGCCAAG CCGCACTCCA CAACCACAAC 
TGGAATGTCC AGCAAGCTGT GCATTATCTA AAGGTAGAAC AGTTGTTCTG TTTGGGCCTG 
AGGAGCAGAT CAGAGTGTCT AAAGCTGCTG GAGATGTGTG ACTGGAACCT GGAGGTGGCC 
AGCACTCACA TGCTGGATAA CTATGGATCT ACAACACGAC AAAGACGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tnk2
Entrez Gene ID	100695859
Full Name	tyrosine kinase non receptor 2
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tnk2 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene

Clones

gRNAs

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