il-6 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	il-6
Entrez Gene ID	100136689
Full Name	interleukin-6
Synonyms	il6
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124657.1	NP_001118129.1	interleukin-6 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Distinct Differentiation Programs Triggered by IL-6 and LPS in Teleost IgM(+) B Cells in The Absence of Germinal Centers.
Ab?s B, Wang T, Castro R, Granja AG, Leal E, Havixbeck J, Luque A, Barreda DR, Secombes CJ, Tafalla C
Scientific reports630004(2016 Aug)

Cloning and characterization of the proximal promoter region of rainbow trout (Oncorhynchus mykiss) interleukin-6 gene.
Zante MD, Borchel A, Brunner RM, Goldammer T, Rebl A
Fish & shellfish immunology43(1)249-56(2015 Mar)

Bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-6: effects on macrophage growth and antimicrobial peptide gene expression.
Costa MM, Maehr T, Diaz-Rosales P, Secombes CJ, Wang T
Molecular immunology48(15-16)1903-16(2011 Sep)

Peptidoglycan, not endotoxin, is the key mediator of cytokine gene expression induced in rainbow trout macrophages by crude LPS.
MacKenzie SA, Roher N, Bolta?a S, Goetz FW
Molecular immunology47(7-8)1450-7(2010 Apr)

Cloning and expression analysis of an IL-6 homolog in rainbow trout (Oncorhynchus mykiss).
Iliev DB, Castellana B, Mackenzie S, Planas JV, Goetz FW
Molecular immunology44(7)1803-7(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IL6 mRNA abundance was significantly increased in spleen, liver, and gill of rainbow trout after experimental infection with Aeromonas salmonicida.
Title: Cloning and characterization of the proximal promoter region of rainbow trout (Oncorhynchus mykiss) interleukin-6 gene.

in this paper we present for the first time in fish the functional characterisation of IL-6, using rainbow trout
Title: Bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-6: effects on macrophage growth and antimicrobial peptide gene expression.

found that peptidoglycans derived from Gram-negative bacteria (Escherichia coli 0111:B4 and K12), are potent inducers of IL-1beta and IL-6 gene expression and were equal to, or more potent than, crude LPS.
Title: Peptidoglycan, not endotoxin, is the key mediator of cytokine gene expression induced in rainbow trout macrophages by crude LPS.

The following il-6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il-6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00097	NM_001124657.1 Latest version!	Oncorhynchus mykiss interleukin-6 (il-6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il-6 ( NM_001124657.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001118129.1 Oncorhynchus mykiss interleukin-6 (il-6), mRNA.

Price & Availability↑

CloneID	OOf00097
Clone ID Related Accession (Same CDS sequence)	NM_001124657.1
Accession Version	NM_001124657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Oncorhynchus mykiss(rainbow trout)
Product	interleukin-6 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ866150.1. ##Evidence-Data-START## Transcript exon combination :: DQ866150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00041049, SAMN00631607 [ECO:0000348] ##Evidence-Data-END##

ATGAATTCTT CTACACGCTA TCTCTCACTC CTCTCGGCGC TCGTGGTGTT AGTTAAGGGG 
AATCCAGTGC CCAGCGCGCT CGCTGAGCTC ATGACCTCCG GATGGACCTC AGGAGAGGAG 
CTCGGGACAG ACGGTGAAAC GGGCGCGCCC CCGAAGTGGG AGAAAATGAT CAAGATGCTC 
GTCCACGAGG TAACCACCTT GCGGAACCAA CAGTTTGTGG AGGAGTTTCA GAAGCCCGTG 
GAAGAGATTT CATCGTTCTC ACAGCACCAG GTCCCATCCA CCCCCCCACA CCTCTCCAAG 
ACTCTTTGCT CCGCCTCCAA CAAGGAGGCA TGTCTGCAGG AGATCAGTCG GGGTCTGCAA 
GTGTACCAGC TTCTTCTTCA GCACGTTAAG GCTGAATACC CTCAATCAAC CCTACTCCCC 
TCTGTCACAC ACCAGACCAC TGTGCTGATA GGGCTGGTCA AAGACCAGAT GAAGGTTGCT 
GAGGTAGTGG AGGACCTGTC TGCCAGTGAG AGGAAGCGTG TTCTGGGTGA GGTGTCTACG 
GGGACAGAGT GGGAGAGGAA GACCAGCGTT CACGCCATCC TTAGGGAGCT ACGTAACTTC 
CTGGTTGACA CCAAGAGAGC CCTCCGCCGC ATGGGAAAGA GGGGCAAAGA CTTCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001118129.1
CDS	79..738
Translation	MNSSTRYLSLLSALVVLVKGNPVPSALAELMTSGWTSGEELGTDGETGAPPKWEKMIKMLVHEVTTLRNQQFVEEFQKPVEEISSFSQHQVPSTPPHLSKTLCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKDQMKVAEVVEDLSASERKRVLGEVSTGTEWERKTSVHAILRELRNFLVDTKRALRRMGKRGKDFQ

Target ORF information:

RefSeq Version	NM_001124657.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss interleukin-6 (il-6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124657.1

ORF Insert Sequence:

ATGAATTCTT CTACACGCTA TCTCTCACTC CTCTCGGCGC TCGTGGTGTT AGTTAAGGGG 
AATCCAGTGC CCAGCGCGCT CGCTGAGCTC ATGACCTCCG GATGGACCTC AGGAGAGGAG 
CTCGGGACAG ACGGTGAAAC GGGCGCGCCC CCGAAGTGGG AGAAAATGAT CAAGATGCTC 
GTCCACGAGG TAACCACCTT GCGGAACCAA CAGTTTGTGG AGGAGTTTCA GAAGCCCGTG 
GAAGAGATTT CATCGTTCTC ACAGCACCAG GTCCCATCCA CCCCCCCACA CCTCTCCAAG 
ACTCTTTGCT CCGCCTCCAA CAAGGAGGCA TGTCTGCAGG AGATCAGTCG GGGTCTGCAA 
GTGTACCAGC TTCTTCTTCA GCACGTTAAG GCTGAATACC CTCAATCAAC CCTACTCCCC 
TCTGTCACAC ACCAGACCAC TGTGCTGATA GGGCTGGTCA AAGACCAGAT GAAGGTTGCT 
GAGGTAGTGG AGGACCTGTC TGCCAGTGAG AGGAAGCGTG TTCTGGGTGA GGTGTCTACG 
GGGACAGAGT GGGAGAGGAA GACCAGCGTT CACGCCATCC TTAGGGAGCT ACGTAACTTC 
CTGGTTGACA CCAAGAGAGC CCTCCGCCGC ATGGGAAAGA GGGGCAAAGA CTTCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distinct Differentiation Programs Triggered by IL-6 and LPS in Teleost IgM(+) B Cells in The Absence of Germinal Centers.
Scientific reports630004(2016 Aug)
Ab?s B,Wang T,Castro R,Granja AG,Leal E,Havixbeck J,Luque A,Barreda DR,Secombes CJ,Tafalla C

Cloning and characterization of the proximal promoter region of rainbow trout (Oncorhynchus mykiss) interleukin-6 gene.
Fish & shellfish immunology43(1)249-56(2015 Mar)
Zante MD,Borchel A,Brunner RM,Goldammer T,Rebl A

Bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-6: effects on macrophage growth and antimicrobial peptide gene expression.
Molecular immunology48(15-16)1903-16(2011 Sep)
Costa MM,Maehr T,Diaz-Rosales P,Secombes CJ,Wang T

Peptidoglycan, not endotoxin, is the key mediator of cytokine gene expression induced in rainbow trout macrophages by crude LPS.
Molecular immunology47(7-8)1450-7(2010 Apr)
MacKenzie SA,Roher N,Bolta?a S,Goetz FW

Cloning and expression analysis of an IL-6 homolog in rainbow trout (Oncorhynchus mykiss).
Molecular immunology44(7)1803-7(2007 Mar)
Iliev DB,Castellana B,Mackenzie S,Planas JV,Goetz FW