il-17a cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	il-17a
Entrez Gene ID	100136642
Full Name	interleukin 17A
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124619.2	NP_001118091.1	interleukin 17A precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The rainbow trout genome provides novel insights into evolution after whole-genome duplication in vertebrates.
Berthelot C, Brunet F, Chalopin D, Juanchich A, Bernard M, No?l B, Bento P, Da Silva C, Labadie K, Alberti A, Aury JM, Louis A, Dehais P, Bardou P, Montfort J, Klopp C, Cabau C, Gaspin C, Thorgaard GH, Boussaha M, Quillet E, Guyomard R, Galiana D, Bobe J, Volff JN, Gen?t C, Wincker P, Jaillon O, Roest Crollius H, Guiguen Y
Nature communications53657(2014 Apr)

Cloning and characterization of rainbow trout interleukin-17A/F2 (IL-17A/F2) and IL-17 receptor A: expression during infection and bioactivity of recombinant IL-17A/F2.
Monte MM, Wang T, Holland JW, Zou J, Secombes CJ
Infection and immunity81(1)340-53(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The recombinant IL-17A/F2 protein was produced in Escherichia coli.
Title: Cloning and characterization of rainbow trout interleukin-17A/F2 (IL-17A/F2) and IL-17 receptor A: expression during infection and bioactivity of recombinant IL-17A/F2.

The following il-17a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il-17a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00136	NM_001124619.2 Latest version!	Oncorhynchus mykiss interleukin 17A (il-17a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il-17a ( NM_001124619.2 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001118091.1 Oncorhynchus mykiss interleukin 17A (il-17a), mRNA.

Price & Availability↑

CloneID	OOf00136
Clone ID Related Accession (Same CDS sequence)	NM_001124619.2
Accession Version	NM_001124619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Oncorhynchus mykiss(rainbow trout)
Product	interleukin 17A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ580842.2. On Feb 26, 2012 this sequence version replaced NM_001124619.1. ##Evidence-Data-START## Transcript exon combination :: AJ580842.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00041049, SAMN00631607 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTCA AAAGCAACGT GTCGAAGTAC CTGGTTGTGT GCTGTGTGTC TATGCTGCTG 
GGCCTGACCA TGGCGAAAGG AATGAAGGTG ACAAAGGAGA GGTGTAACGA AACACTGATC 
ATCCCTTCAG ACTTCTACAA GATTCCCACA GAGGAATCAG AGGGAAATGG GAACATTCAC 
ACACGCTCTC TGTCACCCTG GACCTGGAAA AGCACTACAG TGGAGAACCG TATTCCTCAG 
ACCATGTGGG AGGCCGAGTG CAGCTCTATG TACTGTGTCT ACCCCACCAA CAGAAGCCAG 
TACATGCGCT ACAGGCAGAA CTCTGTACCT ATCTACCAGC AGGTCGTGGT ACTCTACACT 
TCAGCCACCA GGAAGTGCTA CAGCGCCTCC TTCCTGTCTG TGGCCGTGGG GTGCACCTGT 
GCCTGGGCAA GGACTACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001118091.1
CDS	14..454
Translation	MELKSNVSKYLVVCCVSMLLGLTMAKGMKVTKERCNETLIIPSDFYKIPTEESEGNGNIHTRSLSPWTWKSTTVENRIPQTMWEAECSSMYCVYPTNRSQYMRYRQNSVPIYQQVVVLYTSATRKCYSASFLSVAVGCTCAWARTT

Target ORF information:

RefSeq Version	NM_001124619.2
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss interleukin 17A (il-17a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124619.2

ORF Insert Sequence:

ATGGAGCTCA AAAGCAACGT GTCGAAGTAC CTGGTTGTGT GCTGTGTGTC TATGCTGCTG 
GGCCTGACCA TGGCGAAAGG AATGAAGGTG ACAAAGGAGA GGTGTAACGA AACACTGATC 
ATCCCTTCAG ACTTCTACAA GATTCCCACA GAGGAATCAG AGGGAAATGG GAACATTCAC 
ACACGCTCTC TGTCACCCTG GACCTGGAAA AGCACTACAG TGGAGAACCG TATTCCTCAG 
ACCATGTGGG AGGCCGAGTG CAGCTCTATG TACTGTGTCT ACCCCACCAA CAGAAGCCAG 
TACATGCGCT ACAGGCAGAA CTCTGTACCT ATCTACCAGC AGGTCGTGGT ACTCTACACT 
TCAGCCACCA GGAAGTGCTA CAGCGCCTCC TTCCTGTCTG TGGCCGTGGG GTGCACCTGT 
GCCTGGGCAA GGACTACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The rainbow trout genome provides novel insights into evolution after whole-genome duplication in vertebrates.
Nature communications53657(2014 Apr)
Berthelot C,Brunet F,Chalopin D,Juanchich A,Bernard M,No?l B,Bento P,Da Silva C,Labadie K,Alberti A,Aury JM,Louis A,Dehais P,Bardou P,Montfort J,Klopp C,Cabau C,Gaspin C,Thorgaard GH,Boussaha M,Quillet E,Guyomard R,Galiana D,Bobe J,Volff JN,Gen?t C,Wincker P,Jaillon O,Roest Crollius H,Guiguen Y

Cloning and characterization of rainbow trout interleukin-17A/F2 (IL-17A/F2) and IL-17 receptor A: expression during infection and bioactivity of recombinant IL-17A/F2.
Infection and immunity81(1)340-53(2013 Jan)
Monte MM,Wang T,Holland JW,Zou J,Secombes CJ