LOC109883650 cDNA ORF clone, Oncorhynchus kisutch(coho salmon)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109883650
Entrez Gene ID	109883650
Full Name	neurofilament heavy polypeptide-like
Gene Type	protein-coding
Organism	Oncorhynchus kisutch(coho salmon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020475682.1	XP_020331271.1	neurofilament heavy polypeptide-like
XM_031791683.1	XP_031647543.1	neurofilament heavy polypeptide-like

Pathways from BioSystems
Gene Ontology

The following LOC109883650 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109883650 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOn98569	XM_031791683.1 Latest version!	Oncorhynchus kisutch neurofilament heavy polypeptide-like (LOC109883650), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOn05939	XM_020475682.1 Latest version!	Oncorhynchus kisutch neurofilament heavy polypeptide-like (LOC109883650), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109883650 ( XM_031791683.1 ) cDNA ORF clone, Oncorhynchus kisutch(coho salmon) -> XP_031647543.1 Oncorhynchus kisutch neurofilament heavy polypeptide-like (LOC109883650), mRNA.

Price & Availability↑

CloneID	OOn98569
Clone ID Related Accession (Same CDS sequence)	XM_031791683.1
Accession Version	XM_031791683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-08
Organism	Oncorhynchus kisutch(coho salmon)
Product	neurofilament heavy polypeptide-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034189.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oncorhynchus kisutch Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG AACAGAAGAG CTTTGCTATG TTGACCAACT ACACAGAGTC TCCTCCTAAA 
CTACTAGACT GTCCCCTCAC CTTCATGCTG ACCCGCCCTG ATACAAACCT CCTCCAGGAG 
GGTGTGTACA CCACTCTGAA GGGCCTCACC ACTACGGATC CAACGTTGAA GGAACGTCTT 
CTGATTGTCA GTAACGTCCC CACAGGGACT GCAGCAGCCC AGGAGGTTCA CAACCTCGTC 
AAACGCTTCG GCTCCTACCT CGACTCGCTG TCTCTGATCA ACAGGATCTA TTTTGAGATG 
GAATCGTCGT CGGTGGCGAA GACCGTGTGT CTTCATTTCC AGAAGTCCCC CAGTGTAGTT 
CAGAACAACC CTCTGAAGTT CAACTTGCTG GTCAAGGATC AACAGGCACC GCAGCCCCCA 
GCCCAGAAGA CATCTGTTGT CAGCACCAAA GCCAAGAAGA AACCAATCAA AGGCAAGAAG 
CCTGTAGCTA AAGATGTCAC AACCCCAGTT AAACCTGTTA CTCCCACTAC TACCAGCACC 
AATGCTACAG TCTCATCCTC TCAGCCTGTT GCCGTGGCGA CCAAGCAACC GGGTGACGAC 
GTCATCGTCG CGGAGGCCAA GCAACCAGGA AGTGACGTCG TGGTGATGAA TTCCGACGGA 
AAGGCAACGG TGGCGGAGTC TGGAGGGAAA GAGGGTGGGA TTTCCTCGAC AAAAGATGGG 
AAGAAGAAGA ACGCTCATCC TCCCAAAGAG GATGGAGGGA TGAAAACTGA GGAGGAGGCA 
AAAGGAAACA AGGAAGACAC TGCGACCGTC GACATTGTCG TTTCCGCCTC GTTACCTACG 
GTTGCCGTAT CCGCTCCCGA GGAAGAGGTC GCCATGGAAA CCCAGAGCAC CGACTGCGTC 
GCCTCGACCA ATGAGGTTGC ATCAGAACCC GAACAGGTTC TAGCTGCGGT CCCACAACAG 
GGCAGTGATG TCATTGCGTT GGCTAGTGAA ACCGTGATGA CTGTGGCCAC AACAGAACCT 
TGTTTGATCT CCGCTGTGGA GGGATCTGAT AACGTGGCCA ATCCCGTCGA CTCTGACACC 
GTTGCTGCCT TTGTACCGGC ACAAACACCG GCTCCTGTCC CGGTAGAGTT AAATGTTGAA 
GACAAACCTC AGGACTTTCC TCCGGTCACC GAGGAGATCC TCAAGGCCCT GGAGGCAGCG 
GTGCACCAAC ACCGTATGGG GAGACTGGCG GAGGAACAGG CGAAACAGGA ACACAACCAG 
GGAGAGGGGA TCCAGGCCAA GCCTCTACCC AACCAGGAGA GCCCTGCAGC TGGGGAGACG 
GCGGAGAGAA AGACCCCGGC TGAGTCAGAA GAGAAGACTG GGGAGAAAGA GACGGCGAAG 
AAAGAGGAGC CACGACCAGA GAAGTCACAG CCGGACAAGA AGACCACCGA CACGAAGAAG 
ACACCTTCAG GATCTGGCCA ACCAGACCAC AAGAAGACCA CCGACACGAA GAAGACACCT 
TCAGGATCTG GCAAACCAGA CCACAAGAAT GGGAAGACCG AGGCTTCCGG AGGGAGAGGC 
CAGCGCCTCT CCCCAGAAAG GAGAGACTCT TCAAGGCATA GAAGCAGCCG GGCTCCTTCC 
GAAGAGGAGC AGGATCCTTC GACGTCTAGA CAGGGTAACT CCTCTAGCTC CTCTTCTGGC 
TCCCGCAGGA GCAGCAGACA CGACGGCAGC CCCGCCAAGA AGAAGGGGAG GAAAGAAGAG 
GAAGACGAGA GGAGCAAGAG CCTCGGTAGC AGCAGTAAAA CGACCCTCTC CTCTAGGAGC 
AGGTCTAAAG ACACGTACGC GGGAGATGAG TTTACATCTG ACGTCATCCC CGATGAAGCC 
AACTCCTTCG ATCTGGACCA GTTCAACATG GACCAGTTTG TTACTGTGGA CGAGGTGGAA 
GGTGATGAGG CTGAGAACAC CAGCCCACCG CAGTCATTCT CATCTCCGTC GCACAAGACA 
CAAGATAAGG CCTCGGAAAG GTCAAAACGA TCGTCCAAAC GCAGGAGGGG TACCCCAGAT 
ACCCCATCTC CGAAATCTAG CCTGGAGCAC AAGGAGACAC CCACGTCCTC CTCCTCTACC 
CCCCCTGCTC AGAAGACTCC ACGAAAAGCC GCAGCAAAAA AGGCAGCAGC TAAACCTCAA 
CCACCTGCCA CCTCTGGACG CAAGACCAGG TCATCAGCAG CTGTTGTAGA AGCCGCAGAA 
ACCCAGGAAG CTGCCGACGA CAACACCAGC ATAGCCGATA CGGAGGCGGA GCCTGTTCCC 
TTGGAGACTC GGTCTCAGTC GGAAGAGGTT GTCGTCACTA AGGGATGTGA GACGGAGGAT 
GTAGTACCGT CATCCCACCA CAAGATGTCA GTGTTGGACA CTGCATTGAC CGACAACAAG 
GAAGAGAAAA GTGCTGTGTC AGAAAGCGAT ATGGAGACGA CCGCTGAAAA ACCTCCAGAG 
CTAGAGGGGA GCGAGGAGAC GACCCAGGCT GAAAAAAGTT CCTCGATTCT GGGTACCGAG 
GCTCAGGATG GAGCCTGTCC CCTGGAGCTT GGTCTGACCC ATCCGTCTGC CTCAGCCAAG 
GAGCGAAACC TTCAGAAGGC CCTGCAACTA CAAGAGGTGG AGACGGTGTC GGGTCACCAG 
GTGCCTCAAT CACTTATTGA TAAAGTACCT GATGATGGTG ATTTCCAGAT ACTCGACGAA 
GCTGTGGACG ATGACACACC TAGAGACGGA GAAGAGGGCA CTCAAGAACA GTCTGGATCT 
CTATCTCTGG GTTACCAGGC GCCTGCAGCC TCCGAATCTA CAGGTTCCCG GGTGCCTCAA 
GCTACACTAG AGGAACACGA AGAGATCTCC AACGACGAAG AAGAAGAATC TGCTTTCCAG 
ATCCTCGACT CTCTCGAAGA CTCTGAAGAC AACATGGCCG ACTCCTCTCT CGCAGGCCAA 
GGAGGCCGAA GAGATTCTCT ACCCAGAGAG ATGGAGGGGT TCCAGATCCT AGATTCAGTC 
GACGATCAGA CGGAAACGAC CCAAGTTCTG GACAACCATA CGACTGACTC TAAATGTCAG 
GGGGGCTCCA AGCCTCTAAA GGGGGGTAAG AAGACACCCC AAAGAAGGGG AAAAAGGAGA 
TTAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031647543.1
CDS	47..3178
Translation	MEREQKSFAMLTNYTESPPKLLDCPLTFMLTRPDTNLLQEGVYTTLKGLTTTDPTLKERLLIVSNVPTGTAAAQEVHNLVKRFGSYLDSLSLINRIYFEMESSSVAKTVCLHFQKSPSVVQNNPLKFNLLVKDQQAPQPPAQKTSVVSTKAKKKPIKGKKPVAKDVTTPVKPVTPTTTSTNATVSSSQPVAVATKQPGDDVIVAEAKQPGSDVVVMNSDGKATVAESGGKEGGISSTKDGKKKNAHPPKEDGGMKTEEEAKGNKEDTATVDIVVSASLPTVAVSAPEEEVAMETQSTDCVASTNEVASEPEQVLAAVPQQGSDVIALASETVMTVATTEPCLISAVEGSDNVANPVDSDTVAAFVPAQTPAPVPVELNVEDKPQDFPPVTEEILKALEAAVHQHRMGRLAEEQAKQEHNQGEGIQAKPLPNQESPAAGETAERKTPAESEEKTGEKETAKKEEPRPEKSQPDKKTTDTKKTPSGSGQPDHKKTTDTKKTPSGSGKPDHKNGKTEASGGRGQRLSPERRDSSRHRSSRAPSEEEQDPSTSRQGNSSSSSSGSRRSSRHDGSPAKKKGRKEEEDERSKSLGSSSKTTLSSRSRSKDTYAGDEFTSDVIPDEANSFDLDQFNMDQFVTVDEVEGDEAENTSPPQSFSSPSHKTQDKASERSKRSSKRRRGTPDTPSPKSSLEHKETPTSSSSTPPAQKTPRKAAAKKAAAKPQPPATSGRKTRSSAAVVEAAETQEAADDNTSIADTEAEPVPLETRSQSEEVVVTKGCETEDVVPSSHHKMSVLDTALTDNKEEKSAVSESDMETTAEKPPELEGSEETTQAEKSSSILGTEAQDGACPLELGLTHPSASAKERNLQKALQLQEVETVSGHQVPQSLIDKVPDDGDFQILDEAVDDDTPRDGEEGTQEQSGSLSLGYQAPAASESTGSRVPQATLEEHEEISNDEEEESAFQILDSLEDSEDNMADSSLAGQGGRRDSLPREMEGFQILDSVDDQTETTQVLDNHTTDSKCQGGSKPLKGGKKTPQRRGKRRLRR

Target ORF information:

RefSeq Version	XM_031791683.1
Organism	Oncorhynchus kisutch(coho salmon)
Definition	Oncorhynchus kisutch neurofilament heavy polypeptide-like (LOC109883650), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031791683.1

ORF Insert Sequence:

ATGGAGAGAG AACAGAAGAG CTTTGCTATG TTGACCAACT ACACAGAGTC TCCTCCTAAA 
CTACTAGACT GTCCCCTCAC CTTCATGCTG ACCCGCCCTG ATACAAACCT CCTCCAGGAG 
GGTGTGTACA CCACTCTGAA GGGCCTCACC ACTACGGATC CAACGTTGAA GGAACGTCTT 
CTGATTGTCA GTAACGTCCC CACAGGGACT GCAGCAGCCC AGGAGGTTCA CAACCTCGTC 
AAACGCTTCG GCTCCTACCT CGACTCGCTG TCTCTGATCA ACAGGATCTA TTTTGAGATG 
GAATCGTCGT CGGTGGCGAA GACCGTGTGT CTTCATTTCC AGAAGTCCCC CAGTGTAGTT 
CAGAACAACC CTCTGAAGTT CAACTTGCTG GTCAAGGATC AACAGGCACC GCAGCCCCCA 
GCCCAGAAGA CATCTGTTGT CAGCACCAAA GCCAAGAAGA AACCAATCAA AGGCAAGAAG 
CCTGTAGCTA AAGATGTCAC AACCCCAGTT AAACCTGTTA CTCCCACTAC TACCAGCACC 
AATGCTACAG TCTCATCCTC TCAGCCTGTT GCCGTGGCGA CCAAGCAACC GGGTGACGAC 
GTCATCGTCG CGGAGGCCAA GCAACCAGGA AGTGACGTCG TGGTGATGAA TTCCGACGGA 
AAGGCAACGG TGGCGGAGTC TGGAGGGAAA GAGGGTGGGA TTTCCTCGAC AAAAGATGGG 
AAGAAGAAGA ACGCTCATCC TCCCAAAGAG GATGGAGGGA TGAAAACTGA GGAGGAGGCA 
AAAGGAAACA AGGAAGACAC TGCGACCGTC GACATTGTCG TTTCCGCCTC GTTACCTACG 
GTTGCCGTAT CCGCTCCCGA GGAAGAGGTC GCCATGGAAA CCCAGAGCAC CGACTGCGTC 
GCCTCGACCA ATGAGGTTGC ATCAGAACCC GAACAGGTTC TAGCTGCGGT CCCACAACAG 
GGCAGTGATG TCATTGCGTT GGCTAGTGAA ACCGTGATGA CTGTGGCCAC AACAGAACCT 
TGTTTGATCT CCGCTGTGGA GGGATCTGAT AACGTGGCCA ATCCCGTCGA CTCTGACACC 
GTTGCTGCCT TTGTACCGGC ACAAACACCG GCTCCTGTCC CGGTAGAGTT AAATGTTGAA 
GACAAACCTC AGGACTTTCC TCCGGTCACC GAGGAGATCC TCAAGGCCCT GGAGGCAGCG 
GTGCACCAAC ACCGTATGGG GAGACTGGCG GAGGAACAGG CGAAACAGGA ACACAACCAG 
GGAGAGGGGA TCCAGGCCAA GCCTCTACCC AACCAGGAGA GCCCTGCAGC TGGGGAGACG 
GCGGAGAGAA AGACCCCGGC TGAGTCAGAA GAGAAGACTG GGGAGAAAGA GACGGCGAAG 
AAAGAGGAGC CACGACCAGA GAAGTCACAG CCGGACAAGA AGACCACCGA CACGAAGAAG 
ACACCTTCAG GATCTGGCCA ACCAGACCAC AAGAAGACCA CCGACACGAA GAAGACACCT 
TCAGGATCTG GCAAACCAGA CCACAAGAAT GGGAAGACCG AGGCTTCCGG AGGGAGAGGC 
CAGCGCCTCT CCCCAGAAAG GAGAGACTCT TCAAGGCATA GAAGCAGCCG GGCTCCTTCC 
GAAGAGGAGC AGGATCCTTC GACGTCTAGA CAGGGTAACT CCTCTAGCTC CTCTTCTGGC 
TCCCGCAGGA GCAGCAGACA CGACGGCAGC CCCGCCAAGA AGAAGGGGAG GAAAGAAGAG 
GAAGACGAGA GGAGCAAGAG CCTCGGTAGC AGCAGTAAAA CGACCCTCTC CTCTAGGAGC 
AGGTCTAAAG ACACGTACGC GGGAGATGAG TTTACATCTG ACGTCATCCC CGATGAAGCC 
AACTCCTTCG ATCTGGACCA GTTCAACATG GACCAGTTTG TTACTGTGGA CGAGGTGGAA 
GGTGATGAGG CTGAGAACAC CAGCCCACCG CAGTCATTCT CATCTCCGTC GCACAAGACA 
CAAGATAAGG CCTCGGAAAG GTCAAAACGA TCGTCCAAAC GCAGGAGGGG TACCCCAGAT 
ACCCCATCTC CGAAATCTAG CCTGGAGCAC AAGGAGACAC CCACGTCCTC CTCCTCTACC 
CCCCCTGCTC AGAAGACTCC ACGAAAAGCC GCAGCAAAAA AGGCAGCAGC TAAACCTCAA 
CCACCTGCCA CCTCTGGACG CAAGACCAGG TCATCAGCAG CTGTTGTAGA AGCCGCAGAA 
ACCCAGGAAG CTGCCGACGA CAACACCAGC ATAGCCGATA CGGAGGCGGA GCCTGTTCCC 
TTGGAGACTC GGTCTCAGTC GGAAGAGGTT GTCGTCACTA AGGGATGTGA GACGGAGGAT 
GTAGTACCGT CATCCCACCA CAAGATGTCA GTGTTGGACA CTGCATTGAC CGACAACAAG 
GAAGAGAAAA GTGCTGTGTC AGAAAGCGAT ATGGAGACGA CCGCTGAAAA ACCTCCAGAG 
CTAGAGGGGA GCGAGGAGAC GACCCAGGCT GAAAAAAGTT CCTCGATTCT GGGTACCGAG 
GCTCAGGATG GAGCCTGTCC CCTGGAGCTT GGTCTGACCC ATCCGTCTGC CTCAGCCAAG 
GAGCGAAACC TTCAGAAGGC CCTGCAACTA CAAGAGGTGG AGACGGTGTC GGGTCACCAG 
GTGCCTCAAT CACTTATTGA TAAAGTACCT GATGATGGTG ATTTCCAGAT ACTCGACGAA 
GCTGTGGACG ATGACACACC TAGAGACGGA GAAGAGGGCA CTCAAGAACA GTCTGGATCT 
CTATCTCTGG GTTACCAGGC GCCTGCAGCC TCCGAATCTA CAGGTTCCCG GGTGCCTCAA 
GCTACACTAG AGGAACACGA AGAGATCTCC AACGACGAAG AAGAAGAATC TGCTTTCCAG 
ATCCTCGACT CTCTCGAAGA CTCTGAAGAC AACATGGCCG ACTCCTCTCT CGCAGGCCAA 
GGAGGCCGAA GAGATTCTCT ACCCAGAGAG ATGGAGGGGT TCCAGATCCT AGATTCAGTC 
GACGATCAGA CGGAAACGAC CCAAGTTCTG GACAACCATA CGACTGACTC TAAATGTCAG 
GGGGGCTCCA AGCCTCTAAA GGGGGGTAAG AAGACACCCC AAAGAAGGGG AAAAAGGAGA 
TTAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109883650 ( XM_020475682.1 ) cDNA ORF clone, Oncorhynchus kisutch(coho salmon) -> XP_020331271.1 Oncorhynchus kisutch neurofilament heavy polypeptide-like (LOC109883650), mRNA.

Price & Availability↑

CloneID	OOn05939
Clone ID Related Accession (Same CDS sequence)	XM_020475682.1
Accession Version	XM_020475682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-12
Organism	Oncorhynchus kisutch(coho salmon)
Product	neurofilament heavy polypeptide-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018091541.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus kisutch Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCGT CGTCGGTGGC GAAGACCGTG TGTCTTCATT TCCAGAAGTC CCCCAGTGTA 
GTTCAGAACA ACCCTCTGAA GTTCAACTTG CTGGTCAAGG ATCAACAGGC ACCGCAGCCC 
CCAGCCCAGA AGACATCTGT TGTCAGCACC AAAGCCAAGA AGAAACCAAT CAAAGGCAAG 
AAGCCTGTAG CTAAAGATGT CACAACCCCA GTTAAACCTG TTACTCCCAC TACTACCAGC 
ACCAATGCTA CAGTCTCATC CTCTCAGCCT GTTGCCGTGG CGACCAAGCA ACCGGGTGAC 
GACGTCATCG TCGCGGAGGC CAAGCAACCA GGAAGTGACG TCGTGGTGAT GAATTCCGAC 
GGAAAGGCAA CGGTGGCGGA GTCTGGAGGG AAAGAGGGTG GGATTTCCTC GACAAAAGAT 
GGGAAGAAGA AGAACGCTCA TCCTCCCAAA GAGGATGGAG GGATGAAAAC TGAGGAGGAG 
GCAAAAGGAA ACAAGGAAGA CACTGCGACC GTCGACATTG TCGTTTCCGC CTCGTTACCT 
ACGGTTGCCG TATCCGCTCC CGAGGAAGAG GTCGCCATGG AAACCCAGAG CACCGACTGC 
GTCGCCTCGA CCAATGAGGT TGCATCAGAA CCCGAACAGG TTCTAGCTGC GGTCCCACAA 
CAGGGCAGTG ATGTCATTGC GTTGGCTAGT GAAACCGTGA TGACTGTGGC CACAACAGAA 
CCTTGTTTGA TCTCCGCTGT GGAGGGATCT GATAACGTGG CCAATCCCGT CGACTCTGAC 
ACCGTTGCTG CCTTTGTACC GGCACAAACA CCGGCTCCTG TCCCGGTAGA GTTAAATGTT 
GAAGACAAAC CTCAGGACTT TCCTCCGGTC ACCGAGGAGA TCCTCAAGGC CCTGGAGGCA 
GCGGTGCACC AACACCGTAT GGGGAGACTG GCGGAGGAAC AGGCGAAACA GGAACACAAC 
CAGGGAGAGG GGATCCAGGC CAAGCCTCTA CCCAACCAGG AGAGCCCTGC AGCTGGGGAG 
ACGGCGGAGA GAAAGACCCC GGCTGAGTCA GAAGAGAAGA CTGGGGAGAA AGAGACGGCG 
AAGAAAGAGG AGCCACGACC AGAGAAGTCA CAGCCGGACA AGAAGACCAC CGACACGAAG 
AAGACACCTT CAGGATCTGG CCAACCAGAC CACAAGAAGA CCACCGACAC GAAGAAGACA 
CCTTCAGGAT CTGGCAAACC AGACCACAAG AATGGGAAGA CCGAGGCTTC CGGAGGGAGA 
GGCCAGCGCC TCTCCCCAGA AAGGAGAGAC TCTTCAAGGC ATAGAAGCAG CCGGGCTCCT 
TCCGAAGAGG AGCAGGATCC TTCGACGTCT AGACAGGGTA ACTCCTCTAG CTCCTCTTCT 
GGCTCCCGCA GGAGCAGCAG ACACGACGGC AGCCCCGCCA AGAAGAAGGG GAGGAAAGAA 
GAGGAAGACG AGAGGAGCAA GAGCCTCGGT AGCAGCAGTA AAACGACCCT CTCCTCTAGG 
AGCAGGTCTA AAGACACGTA CGCGGGAGAT GAGTTTACAT CTGACGTCAT CCCCGATGAA 
GCCAACTCCT TCGATCTGGA CCAGTTCAAC ATGGACCAGT TTGTTACTGT GGACGAGGTG 
GAAGGTGATG AGGCTAAGAA CACCAACCCA CCGCAGTCAT CCTCATCTTC GTCACACAAT 
ACACAAGATA AGGCCTCGGA AAGGTCAAAA CGATCGTCCA AACGCAYGAG GGGTACCCCA 
GATACCCCAT CTCCGAAATC TAGCCTGGAG CACAAGGAGA CACCCACGTC CTCCTCCTCT 
ACCCCCCCTG CTCAGAAGAC TCCACGAAAA GCCGCAGCAA AAAAGGCGTC AGCTAAACCT 
CAACCACCTG CCACCTCTGG ACGCAAGACC AGGTCATCAG CAGCTGTTGT AGAAGCCGCA 
GAAACCCAGG AAGCTGCCGA CGACAACACC AGCATAGCCG ATACGGAGGC GGAGCCTGTT 
CCCTTGGAGA CTCGGTCTCA GTCGGAAGAG GTTGTCGTCA CTAAGGGATG TGAGACGGAG 
GATGTAGTAC CGTCATCCCA CCACAAGATG TCAGTGTTGG ACACTGCATT GACCGACAAC 
AAGGAAGAGA AAAGTGCTGT GTCAGAAAGC GATATGGAGA CGACCGCTGA AAAACCTCCA 
GAGCTAGAGG GGAGCGAGGA GACGACCCAG GCTGAAAAAA GTTCCTCGAT TCTGGGTACC 
GAGGCTCAGG ATGGAGCCTG TCCCCTGGAG CTTGGTCTGA CCCATCCGTC TGCCTCAGCC 
AAGGAGCGAA ACCTTCAGAA GGCCCTGCAA CTACAAGAGG TGGAGACGGT GTCGGGTCAC 
CAGGTGCCTC AATCACTTAT TGATAAAGTA CCTGATGATG GTGATTTCCA GATACTCGAC 
GAAGCTGTGG ACGATGACAC ACCTAGAGAC GGAGAAGAGG GCACTCAAGA ACAGTCTGGA 
TCTCTATCTC TGGGTTACCA GGCGCCTGCA GCCTCCGAAT CTACAGGTTC CCGGGTGCCT 
CAAGCTACAC TAGAGGAACA CGAAGAGATC TCCAACGACY AAGAAGAAGA ATCTGCTTTC 
CAGATCCTCG ACTCTCTCGA AGACTCTGAA GACAACATGG CCGACTCCTC TCTCGCAGGC 
CAAGGAGGCC GAAGAGATTC TCTACCCAGA GAGATGGAGG GGTTCCAGAT KCTAGATTCA 
GTCGACGATC AGACGGAAAC GACCCAAGTT CTGGACAACC ATACGACTGA CTCTAAATGT 
CAGGGGGGCT CCAAGCCTCT AAAGGGGGGT AAGAAGACAC CCCAAAAGAA GGGGAAAAAG 
GAGATTAAGA AGGTATCCAA AACTCAAGAG GTGACCAAGA CCCCAACAGG AGAGTCCGAG 
GGGGGTACCC CGAAAAAGAG TAACCTTGTC ACCTTGGATG AGGTGAGCGA GGAGGAGGAG 
GATTACCCAG ATGACGCCGC CGAAGAGGAA GAACTTATTA AGAAACAAAA ACTGGTGAAG 
GAGAAGCAGC GAGAGAAGGA CAGAGGGAAG GAGAGAGAGA AAGGGCGGAG GAGGAGTAGA 
GAGAAGGAAC GGACTAGGGA GGAGGAGAGG GTCGGAGTGG ATACTGAGGG CTTGGTGACT 
TTGGATGAGA TTGGAGAGGA TGAAGGAGCG GAGGGGCCTT GCCTGGAGTC AGGAGACACC 
TTCAACCCAG AGACTCTGGT AACCCTGGTG AGGCTGGAGG TGATGGGGAG GATGATGAGG 
AGGAAGAGCA GAACAAGGGT CCTGAACCGC GCAGAACCAG CAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020331271.1
CDS	153..3440
Translation	MESSSVAKTVCLHFQKSPSVVQNNPLKFNLLVKDQQAPQPPAQKTSVVSTKAKKKPIKGKKPVAKDVTTPVKPVTPTTTSTNATVSSSQPVAVATKQPGDDVIVAEAKQPGSDVVVMNSDGKATVAESGGKEGGISSTKDGKKKNAHPPKEDGGMKTEEEAKGNKEDTATVDIVVSASLPTVAVSAPEEEVAMETQSTDCVASTNEVASEPEQVLAAVPQQGSDVIALASETVMTVATTEPCLISAVEGSDNVANPVDSDTVAAFVPAQTPAPVPVELNVEDKPQDFPPVTEEILKALEAAVHQHRMGRLAEEQAKQEHNQGEGIQAKPLPNQESPAAGETAERKTPAESEEKTGEKETAKKEEPRPEKSQPDKKTTDTKKTPSGSGQPDHKKTTDTKKTPSGSGKPDHKNGKTEASGGRGQRLSPERRDSSRHRSSRAPSEEEQDPSTSRQGNSSSSSSGSRRSSRHDGSPAKKKGRKEEEDERSKSLGSSSKTTLSSRSRSKDTYAGDEFTSDVIPDEANSFDLDQFNMDQFVTVDEVEGDEAKNTNPPQSSSSSSHNTQDKASERSKRSSKRnullRGTPDTPSPKSSLEHKETPTSSSSTPPAQKTPRKAAAKKASAKPQPPATSGRKTRSSAAVVEAAETQEAADDNTSIADTEAEPVPLETRSQSEEVVVTKGCETEDVVPSSHHKMSVLDTALTDNKEEKSAVSESDMETTAEKPPELEGSEETTQAEKSSSILGTEAQDGACPLELGLTHPSASAKERNLQKALQLQEVETVSGHQVPQSLIDKVPDDGDFQILDEAVDDDTPRDGEEGTQEQSGSLSLGYQAPAASESTGSRVPQATLEEHEEISNDnullEEESAFQILDSLEDSEDNMADSSLAGQGGRRDSLPREMEGFQnullLDSVDDQTETTQVLDNHTTDSKCQGGSKPLKGGKKTPQKKGKKEIKKVSKTQEVTKTPTGESEGGTPKKSNLVTLDEVSEEEEDYPDDAAEEEELIKKQKLVKEKQREKDRGKEREKGRRRSREKERTREEERVGVDTEGLVTLDEIGEDEGAEGPCLESGDTFNPETLVTLVRLEVMGRMMRRKSRTRVLNRAEPAS

Target ORF information:

RefSeq Version	XM_020475682.1
Organism	Oncorhynchus kisutch(coho salmon)
Definition	Oncorhynchus kisutch neurofilament heavy polypeptide-like (LOC109883650), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020475682.1

ORF Insert Sequence:

ATGGAATCGT CGTCGGTGGC GAAGACCGTG TGTCTTCATT TCCAGAAGTC CCCCAGTGTA 
GTTCAGAACA ACCCTCTGAA GTTCAACTTG CTGGTCAAGG ATCAACAGGC ACCGCAGCCC 
CCAGCCCAGA AGACATCTGT TGTCAGCACC AAAGCCAAGA AGAAACCAAT CAAAGGCAAG 
AAGCCTGTAG CTAAAGATGT CACAACCCCA GTTAAACCTG TTACTCCCAC TACTACCAGC 
ACCAATGCTA CAGTCTCATC CTCTCAGCCT GTTGCCGTGG CGACCAAGCA ACCGGGTGAC 
GACGTCATCG TCGCGGAGGC CAAGCAACCA GGAAGTGACG TCGTGGTGAT GAATTCCGAC 
GGAAAGGCAA CGGTGGCGGA GTCTGGAGGG AAAGAGGGTG GGATTTCCTC GACAAAAGAT 
GGGAAGAAGA AGAACGCTCA TCCTCCCAAA GAGGATGGAG GGATGAAAAC TGAGGAGGAG 
GCAAAAGGAA ACAAGGAAGA CACTGCGACC GTCGACATTG TCGTTTCCGC CTCGTTACCT 
ACGGTTGCCG TATCCGCTCC CGAGGAAGAG GTCGCCATGG AAACCCAGAG CACCGACTGC 
GTCGCCTCGA CCAATGAGGT TGCATCAGAA CCCGAACAGG TTCTAGCTGC GGTCCCACAA 
CAGGGCAGTG ATGTCATTGC GTTGGCTAGT GAAACCGTGA TGACTGTGGC CACAACAGAA 
CCTTGTTTGA TCTCCGCTGT GGAGGGATCT GATAACGTGG CCAATCCCGT CGACTCTGAC 
ACCGTTGCTG CCTTTGTACC GGCACAAACA CCGGCTCCTG TCCCGGTAGA GTTAAATGTT 
GAAGACAAAC CTCAGGACTT TCCTCCGGTC ACCGAGGAGA TCCTCAAGGC CCTGGAGGCA 
GCGGTGCACC AACACCGTAT GGGGAGACTG GCGGAGGAAC AGGCGAAACA GGAACACAAC 
CAGGGAGAGG GGATCCAGGC CAAGCCTCTA CCCAACCAGG AGAGCCCTGC AGCTGGGGAG 
ACGGCGGAGA GAAAGACCCC GGCTGAGTCA GAAGAGAAGA CTGGGGAGAA AGAGACGGCG 
AAGAAAGAGG AGCCACGACC AGAGAAGTCA CAGCCGGACA AGAAGACCAC CGACACGAAG 
AAGACACCTT CAGGATCTGG CCAACCAGAC CACAAGAAGA CCACCGACAC GAAGAAGACA 
CCTTCAGGAT CTGGCAAACC AGACCACAAG AATGGGAAGA CCGAGGCTTC CGGAGGGAGA 
GGCCAGCGCC TCTCCCCAGA AAGGAGAGAC TCTTCAAGGC ATAGAAGCAG CCGGGCTCCT 
TCCGAAGAGG AGCAGGATCC TTCGACGTCT AGACAGGGTA ACTCCTCTAG CTCCTCTTCT 
GGCTCCCGCA GGAGCAGCAG ACACGACGGC AGCCCCGCCA AGAAGAAGGG GAGGAAAGAA 
GAGGAAGACG AGAGGAGCAA GAGCCTCGGT AGCAGCAGTA AAACGACCCT CTCCTCTAGG 
AGCAGGTCTA AAGACACGTA CGCGGGAGAT GAGTTTACAT CTGACGTCAT CCCCGATGAA 
GCCAACTCCT TCGATCTGGA CCAGTTCAAC ATGGACCAGT TTGTTACTGT GGACGAGGTG 
GAAGGTGATG AGGCTAAGAA CACCAACCCA CCGCAGTCAT CCTCATCTTC GTCACACAAT 
ACACAAGATA AGGCCTCGGA AAGGTCAAAA CGATCGTCCA AACGCAYGAG GGGTACCCCA 
GATACCCCAT CTCCGAAATC TAGCCTGGAG CACAAGGAGA CACCCACGTC CTCCTCCTCT 
ACCCCCCCTG CTCAGAAGAC TCCACGAAAA GCCGCAGCAA AAAAGGCGTC AGCTAAACCT 
CAACCACCTG CCACCTCTGG ACGCAAGACC AGGTCATCAG CAGCTGTTGT AGAAGCCGCA 
GAAACCCAGG AAGCTGCCGA CGACAACACC AGCATAGCCG ATACGGAGGC GGAGCCTGTT 
CCCTTGGAGA CTCGGTCTCA GTCGGAAGAG GTTGTCGTCA CTAAGGGATG TGAGACGGAG 
GATGTAGTAC CGTCATCCCA CCACAAGATG TCAGTGTTGG ACACTGCATT GACCGACAAC 
AAGGAAGAGA AAAGTGCTGT GTCAGAAAGC GATATGGAGA CGACCGCTGA AAAACCTCCA 
GAGCTAGAGG GGAGCGAGGA GACGACCCAG GCTGAAAAAA GTTCCTCGAT TCTGGGTACC 
GAGGCTCAGG ATGGAGCCTG TCCCCTGGAG CTTGGTCTGA CCCATCCGTC TGCCTCAGCC 
AAGGAGCGAA ACCTTCAGAA GGCCCTGCAA CTACAAGAGG TGGAGACGGT GTCGGGTCAC 
CAGGTGCCTC AATCACTTAT TGATAAAGTA CCTGATGATG GTGATTTCCA GATACTCGAC 
GAAGCTGTGG ACGATGACAC ACCTAGAGAC GGAGAAGAGG GCACTCAAGA ACAGTCTGGA 
TCTCTATCTC TGGGTTACCA GGCGCCTGCA GCCTCCGAAT CTACAGGTTC CCGGGTGCCT 
CAAGCTACAC TAGAGGAACA CGAAGAGATC TCCAACGACY AAGAAGAAGA ATCTGCTTTC 
CAGATCCTCG ACTCTCTCGA AGACTCTGAA GACAACATGG CCGACTCCTC TCTCGCAGGC 
CAAGGAGGCC GAAGAGATTC TCTACCCAGA GAGATGGAGG GGTTCCAGAT KCTAGATTCA 
GTCGACGATC AGACGGAAAC GACCCAAGTT CTGGACAACC ATACGACTGA CTCTAAATGT 
CAGGGGGGCT CCAAGCCTCT AAAGGGGGGT AAGAAGACAC CCCAAAAGAA GGGGAAAAAG 
GAGATTAAGA AGGTATCCAA AACTCAAGAG GTGACCAAGA CCCCAACAGG AGAGTCCGAG 
GGGGGTACCC CGAAAAAGAG TAACCTTGTC ACCTTGGATG AGGTGAGCGA GGAGGAGGAG 
GATTACCCAG ATGACGCCGC CGAAGAGGAA GAACTTATTA AGAAACAAAA ACTGGTGAAG 
GAGAAGCAGC GAGAGAAGGA CAGAGGGAAG GAGAGAGAGA AAGGGCGGAG GAGGAGTAGA 
GAGAAGGAAC GGACTAGGGA GGAGGAGAGG GTCGGAGTGG ATACTGAGGG CTTGGTGACT 
TTGGATGAGA TTGGAGAGGA TGAAGGAGCG GAGGGGCCTT GCCTGGAGTC AGGAGACACC 
TTCAACCCAG AGACTCTGGT AACCCTGGTG AGGCTGGAGG TGATGGGGAG GATGATGAGG 
AGGAAGAGCA GAACAAGGGT CCTGAACCGC GCAGAACCAG CAAGCTGA