CHRNA4 cDNA ORF clone, Odobenus rosmarus divergens(Pacific walrus)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNA4
Entrez Gene ID	101385224
Full Name	cholinergic receptor nicotinic alpha 4 subunit
Gene Type	protein-coding
Organism	Odobenus rosmarus divergens(Pacific walrus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004410127.1	XP_004410184.1	neuronal acetylcholine receptor subunit alpha-4

Pathways from BioSystems
Gene Ontology

The following CHRNA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOd64229	XM_004410127.1 Latest version!	Odobenus rosmarus divergens cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNA4 ( XM_004410127.1 ) cDNA ORF clone, Odobenus rosmarus divergens(Pacific walrus) -> XP_004410184.1 Odobenus rosmarus divergens cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), mRNA.

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CloneID	OOd64229
Clone ID Related Accession (Same CDS sequence)	XM_004410127.1
Accession Version	XM_004410127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Odobenus rosmarus divergens(Pacific walrus)
Product	neuronal acetylcholine receptor subunit alpha-4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004450733.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Odobenus rosmarus divergens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCG GGGGCCCCGG GTCGCCGCCG CCGCCGCTGC TCCCGCCGCT GCTGATGCTG 
CTGGGGGCCG GCTTCCTACC CGTAAGCAGC CACATGGAGA CCCGGGCCCA TGCGGAGGAA 
CGGCTGCTGA AGAAACTCTT CTCTGGCTAC AACAAGTGGT CCCGGCCCGT GGCCAACATC 
TCGGACGTGG TTCTTGTCCA CTTTGGGCTG TCCATCGCAC AGCTCATTGA CGTGGATGAA 
AAGAACCAGA TGATGACAAC CAATGTGTGG GTGAAGCAGG AATGGCGTGA CTATAAGCTG 
CGCTGGGACC CCGCTGACTA CGAGAACGTC ACCTCCATCC GCATTCCCTC TGAGCTCATC 
TGGCGGCCAG ACATCGTCCT CTACAACAAC GCGGACGGAG ACTTTGCGGT CACCCACCTG 
ACCAAGGCCC ACCTCTTCCA CGACGGGCGG GTCCAGTGGA CACCCCCCGC CATCTACAAG 
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCGTTCGACC AGCAGAACTG CACCATGAAG 
TTCGGGTCCT GGACGTACGA CAAGGCCAAG ATCGACTTGG TGAGCATGCA CAGCCGTGTG 
GACCAGCTGG ACCTGTGGGA GAGTGGCGAG TGGGTCATCG TGGACGCTGT GGGCACCTAC 
AACACCCGCA AATACGAGTG CTGCGCCGAG GTCTACCCGG ACATCACCTA CGCCTTCGTC 
ATCCGGCGCC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC 
TGCCTCACGG TGCTGGTCTT CTACCTGCCG TCCGAGTGCG GCGAGAAGAT CACGCTCTGC 
ATCTCCGTGT TGCTCTCGCT CACCGTCTTC CTGCTGCTCA TCACGGAGAT CATCCCCTCC 
ACCTCCCTGG TCATCCCGCT CATCGGCGAG TACCTGCTCT TCACCATGAT CTTCGTCACG 
CTGTCCATTG TCATCACGGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC GCGCACACAC 
ACCATGCCCA CCTGGGTGCG CCGGGTCTTC CTGGATGTGG TGCCACGCTT GCTCTTCATG 
AAGCGGCCGT CTGTGGTCAA GGACAACTGC AGGCGGCTCA TCGAGTCCAT GCACAAGATG 
GCCAGCGCCC CTGGCTTCTG GCCCGAGCCC GAGGGGGAGC CCAGCTTCGT GGGCGGAGCC 
CAGAGCCAGG GCCCCTCGCG CTCCCTGTCC TTCTGCAGCC CCCCGGACGA GCCAGCCGAG 
CCCCAGCCAG CCTGCGAGTC ACCCTCAGAC CAGGTCCCTG TGTTGCTGCC CTCAGAGGCT 
GAGAACGGGA GCCCCTGCTC CCCGCCTGAC CCCCGCCACC CACCCACCAG CACCCAGGCC 
CCAGGGCTCA CCAAAGCCCG GTCTCTGAGC GTCCAGCACA TGTCCAGCCC TGCCGAAGCC 
GCGGAAGTTG GTGTTCGATG CCGGTCTCAG AGCATCCAGT ACTGCGTTCC CCAAGATGAT 
GCCGCCTCCC CGGCTGGGGG CCCTGTGGCC GGCTCCCCTG CCTTTCTGAA GGCCGGCTCC 
GCCGAGCTCC TGCCCCCAGA CCAGCCCTCT CCGTGCAAAT GCAGGTGCAA GAAGGAGCCA 
TCCCCAAATG CCGTGCTCAA AGCCCGGGGC ACCAAAGTGC CCCCCCCGCA CCTGCCCCTG 
TCTCCGGCCC TGACGCGGGC GGTCGAGGGT GTCCAGTACA TCGCAGACCA TCTGAAGGCC 
GAAGACACAG ACTTCTCGGT GAAGGAGGAT TGGAAGTACG TGGCCATGGT CATCGACCGC 
ATCTTCCTCT GGGTGTTCAT CATCGTCTGC CTGCTGGGCA CCGCGGGCCT CTTCCTGCCA 
CCCTGGCTGG CCGGCATGAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004410184.1
CDS	257..2140
Translation	MELGGPGSPPPPLLPPLLMLLGAGFLPVSSHMETRAHAEERLLKKLFSGYNKWSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWRDYKLRWDPADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSMHSRVDQLDLWESGEWVIVDAVGTYNTRKYECCAEVYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDVVPRLLFMKRPSVVKDNCRRLIESMHKMASAPGFWPEPEGEPSFVGGAQSQGPSRSLSFCSPPDEPAEPQPACESPSDQVPVLLPSEAENGSPCSPPDPRHPPTSTQAPGLTKARSLSVQHMSSPAEAAEVGVRCRSQSIQYCVPQDDAASPAGGPVAGSPAFLKAGSAELLPPDQPSPCKCRCKKEPSPNAVLKARGTKVPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWVFIIVCLLGTAGLFLPPWLAGMI

Target ORF information:

RefSeq Version	XM_004410127.1
Organism	Odobenus rosmarus divergens(Pacific walrus)
Definition	Odobenus rosmarus divergens cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004410127.1

ORF Insert Sequence:

ATGGAGCTCG GGGGCCCCGG GTCGCCGCCG CCGCCGCTGC TCCCGCCGCT GCTGATGCTG 
CTGGGGGCCG GCTTCCTACC CGTAAGCAGC CACATGGAGA CCCGGGCCCA TGCGGAGGAA 
CGGCTGCTGA AGAAACTCTT CTCTGGCTAC AACAAGTGGT CCCGGCCCGT GGCCAACATC 
TCGGACGTGG TTCTTGTCCA CTTTGGGCTG TCCATCGCAC AGCTCATTGA CGTGGATGAA 
AAGAACCAGA TGATGACAAC CAATGTGTGG GTGAAGCAGG AATGGCGTGA CTATAAGCTG 
CGCTGGGACC CCGCTGACTA CGAGAACGTC ACCTCCATCC GCATTCCCTC TGAGCTCATC 
TGGCGGCCAG ACATCGTCCT CTACAACAAC GCGGACGGAG ACTTTGCGGT CACCCACCTG 
ACCAAGGCCC ACCTCTTCCA CGACGGGCGG GTCCAGTGGA CACCCCCCGC CATCTACAAG 
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCGTTCGACC AGCAGAACTG CACCATGAAG 
TTCGGGTCCT GGACGTACGA CAAGGCCAAG ATCGACTTGG TGAGCATGCA CAGCCGTGTG 
GACCAGCTGG ACCTGTGGGA GAGTGGCGAG TGGGTCATCG TGGACGCTGT GGGCACCTAC 
AACACCCGCA AATACGAGTG CTGCGCCGAG GTCTACCCGG ACATCACCTA CGCCTTCGTC 
ATCCGGCGCC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC 
TGCCTCACGG TGCTGGTCTT CTACCTGCCG TCCGAGTGCG GCGAGAAGAT CACGCTCTGC 
ATCTCCGTGT TGCTCTCGCT CACCGTCTTC CTGCTGCTCA TCACGGAGAT CATCCCCTCC 
ACCTCCCTGG TCATCCCGCT CATCGGCGAG TACCTGCTCT TCACCATGAT CTTCGTCACG 
CTGTCCATTG TCATCACGGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC GCGCACACAC 
ACCATGCCCA CCTGGGTGCG CCGGGTCTTC CTGGATGTGG TGCCACGCTT GCTCTTCATG 
AAGCGGCCGT CTGTGGTCAA GGACAACTGC AGGCGGCTCA TCGAGTCCAT GCACAAGATG 
GCCAGCGCCC CTGGCTTCTG GCCCGAGCCC GAGGGGGAGC CCAGCTTCGT GGGCGGAGCC 
CAGAGCCAGG GCCCCTCGCG CTCCCTGTCC TTCTGCAGCC CCCCGGACGA GCCAGCCGAG 
CCCCAGCCAG CCTGCGAGTC ACCCTCAGAC CAGGTCCCTG TGTTGCTGCC CTCAGAGGCT 
GAGAACGGGA GCCCCTGCTC CCCGCCTGAC CCCCGCCACC CACCCACCAG CACCCAGGCC 
CCAGGGCTCA CCAAAGCCCG GTCTCTGAGC GTCCAGCACA TGTCCAGCCC TGCCGAAGCC 
GCGGAAGTTG GTGTTCGATG CCGGTCTCAG AGCATCCAGT ACTGCGTTCC CCAAGATGAT 
GCCGCCTCCC CGGCTGGGGG CCCTGTGGCC GGCTCCCCTG CCTTTCTGAA GGCCGGCTCC 
GCCGAGCTCC TGCCCCCAGA CCAGCCCTCT CCGTGCAAAT GCAGGTGCAA GAAGGAGCCA 
TCCCCAAATG CCGTGCTCAA AGCCCGGGGC ACCAAAGTGC CCCCCCCGCA CCTGCCCCTG 
TCTCCGGCCC TGACGCGGGC GGTCGAGGGT GTCCAGTACA TCGCAGACCA TCTGAAGGCC 
GAAGACACAG ACTTCTCGGT GAAGGAGGAT TGGAAGTACG TGGCCATGGT CATCGACCGC 
ATCTTCCTCT GGGTGTTCAT CATCGTCTGC CTGCTGGGCA CCGCGGGCCT CTTCCTGCCA 
CCCTGGCTGG CCGGCATGAT CTAG