wls cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wls
Entrez Gene ID	107396595
Full Name	wntless Wnt ligand secretion mediator
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015976367.1	XP_015831853.1	protein wntless homolog

Pathways from BioSystems
Gene Ontology

The following wls gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wls cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo30387	XM_015976367.1 Latest version!	Nothobranchius furzeri wntless Wnt ligand secretion mediator (wls), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wls ( XM_015976367.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015831853.1 Nothobranchius furzeri wntless Wnt ligand secretion mediator (wls), mRNA.

Price & Availability↑

CloneID	ONo30387
Clone ID Related Accession (Same CDS sequence)	XM_015976367.1
Accession Version	XM_015976367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	protein wntless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015444052.1) and transcript sequence (GAIB01109589.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 182 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCGGGCG CTATTATTGA AAATATGAGC ACCAAAAAGT TGGTGATAGT GGGAGTGATT 
CTGCTTTTGT TCCAGGCGTT TGCCTTCATG GTCGGCGGGT TGATTGCTCC CAGCCCCACC 
ACAGCTGTCG ATTACTTAGC GGTCAAGTGT GTGGGCAAGG CCAGTCACCG ACAAGAGACC 
AAGTGGTTCA TGCCTTGGGG TTCCAATCAG TGTGATAAAA TCCAGTCGTT TGACGAGGCC 
GTGGCCAAGA GGATTGAGGC CAACGACATC GTGTTTGCTG TCCACATCCC CATGAGCAAC 
AATGAGATGA GCCCCTGGTT CCAGTTCATA TTGGTCATGC TTCAGTTTGA CATTGCATTC 
AAGATGACCA ACCAGATAAA GGATGGAGCC ATAGTAAACC TTGACGTCAA GCTGGCCTAC 
AGAGATGACA TGGTCAGTGA GTGGAACGAG ATGGCTCGAT CATTTGAAAA GAGGAAACTC 
CACTGCAACT TCACCTTCCC CAAGACCTAC GTGAACGATG GTCGTTTCTA TACATGTGAT 
GTGCTGCCTT TCATGGAGGT GGGGAGCGTT GCCCATAAAT ACTACCTACT CAACATCCGG 
CTGCCTGTCA ACGAGCGCAA GAAGATCAAC CTTGGGATCG GAGACATCAA AGACATTCAT 
CTTGTTGGAA TCCATCAAAA CGGAGGCTTC ACTAAAGTCT GGTTTGCTAT GAAAAGTTTC 
CTCACACCCA GCATCCTCAT CATTATGATC TGGTACTGGA GACGCATCAG CCTCATGAAC 
AGACCGCCTG TCCTCCTGGA GAAGGTGATC TTTGCTTTGG GCATCTCCAT GACGTTCATT 
AACATCCCAG TGGAGTGGTT CTCAGTTGGG TTTAACTGGA CCTGGATGCT GCTGTTTGGA 
GATATCAGAC AGGGAATCTT CTACTCCATG CTCCTGTCCT TCTGGATCAT ATTCTGTGGA 
GAACATCTCA TGGATCAGAC GGAGAGGAAC CGATTTTCTG TCTACTGGAA GCAAGTCGGA 
CCCATCGTGC TAGGTTCCCT CTGCCTCTTC ATCTTTGACA TGTGTGAGAG GGGCGTCCAG 
CTAACGAATC CCTTCTACAG CATCTGGGCC TCGGATGTTG GAACCGAGCT TGCTCTGTCG 
TTCATAATTT TGGCAGGAAT CTGCGCCTGT CTCTACTTCC TCTTCCTTTG TTTCATGGTG 
TTTCAAGTCT TCCGTAACAT CAGTGGGAAG AGGACATCCC TACCTGCCTT GTCTAAGGCC 
AGACGTCTCC ACTATGAGGG TCTCATCTTC AGGTTCAAAT TCCTCATGCT CCTCACTCTG 
TTCTGTGCTG CCATGACTGT CATCTTCTTC ATCATCAGCC AGGTTAACCA GGGTCACTGG 
CACTGGGGCG GACACACGGT GCAGGTGAAC AGTGCCTTCT TCACGGGCAT CTATGGCATG 
TGGAATCTGT ATGTCTTCGC CATCATGTTC CTCTACGCAC CTTCTCACAA ACATTATGGG 
GACGAACAGT CGAGTGGCCA GTGCATCACA GTTGATGAAG GCGTGAAGAG CGGCGAGAAC 
ATCCAGCTGA CCACCACCGT CACCCACGTG GACGGTCCCA CTGAGCTCTA CAGAATGATG 
GGCAAAGAGG CGCAGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015831853.1
CDS	147..1787
Translation	MAGAIIENMSTKKLVIVGVILLLFQAFAFMVGGLIAPSPTTAVDYLAVKCVGKASHRQETKWFMPWGSNQCDKIQSFDEAVAKRIEANDIVFAVHIPMSNNEMSPWFQFILVMLQFDIAFKMTNQIKDGAIVNLDVKLAYRDDMVSEWNEMARSFEKRKLHCNFTFPKTYVNDGRFYTCDVLPFMEVGSVAHKYYLLNIRLPVNERKKINLGIGDIKDIHLVGIHQNGGFTKVWFAMKSFLTPSILIIMIWYWRRISLMNRPPVLLEKVIFALGISMTFINIPVEWFSVGFNWTWMLLFGDIRQGIFYSMLLSFWIIFCGEHLMDQTERNRFSVYWKQVGPIVLGSLCLFIFDMCERGVQLTNPFYSIWASDVGTELALSFIILAGICACLYFLFLCFMVFQVFRNISGKRTSLPALSKARRLHYEGLIFRFKFLMLLTLFCAAMTVIFFIISQVNQGHWHWGGHTVQVNSAFFTGIYGMWNLYVFAIMFLYAPSHKHYGDEQSSGQCITVDEGVKSGENIQLTTTVTHVDGPTELYRMMGKEAQE

Target ORF information:

RefSeq Version	XM_015976367.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri wntless Wnt ligand secretion mediator (wls), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015976367.1

ORF Insert Sequence:

ATGGCGGGCG CTATTATTGA AAATATGAGC ACCAAAAAGT TGGTGATAGT GGGAGTGATT 
CTGCTTTTGT TCCAGGCGTT TGCCTTCATG GTCGGCGGGT TGATTGCTCC CAGCCCCACC 
ACAGCTGTCG ATTACTTAGC GGTCAAGTGT GTGGGCAAGG CCAGTCACCG ACAAGAGACC 
AAGTGGTTCA TGCCTTGGGG TTCCAATCAG TGTGATAAAA TCCAGTCGTT TGACGAGGCC 
GTGGCCAAGA GGATTGAGGC CAACGACATC GTGTTTGCTG TCCACATCCC CATGAGCAAC 
AATGAGATGA GCCCCTGGTT CCAGTTCATA TTGGTCATGC TTCAGTTTGA CATTGCATTC 
AAGATGACCA ACCAGATAAA GGATGGAGCC ATAGTAAACC TTGACGTCAA GCTGGCCTAC 
AGAGATGACA TGGTCAGTGA GTGGAACGAG ATGGCTCGAT CATTTGAAAA GAGGAAACTC 
CACTGCAACT TCACCTTCCC CAAGACCTAC GTGAACGATG GTCGTTTCTA TACATGTGAT 
GTGCTGCCTT TCATGGAGGT GGGGAGCGTT GCCCATAAAT ACTACCTACT CAACATCCGG 
CTGCCTGTCA ACGAGCGCAA GAAGATCAAC CTTGGGATCG GAGACATCAA AGACATTCAT 
CTTGTTGGAA TCCATCAAAA CGGAGGCTTC ACTAAAGTCT GGTTTGCTAT GAAAAGTTTC 
CTCACACCCA GCATCCTCAT CATTATGATC TGGTACTGGA GACGCATCAG CCTCATGAAC 
AGACCGCCTG TCCTCCTGGA GAAGGTGATC TTTGCTTTGG GCATCTCCAT GACGTTCATT 
AACATCCCAG TGGAGTGGTT CTCAGTTGGG TTTAACTGGA CCTGGATGCT GCTGTTTGGA 
GATATCAGAC AGGGAATCTT CTACTCCATG CTCCTGTCCT TCTGGATCAT ATTCTGTGGA 
GAACATCTCA TGGATCAGAC GGAGAGGAAC CGATTTTCTG TCTACTGGAA GCAAGTCGGA 
CCCATCGTGC TAGGTTCCCT CTGCCTCTTC ATCTTTGACA TGTGTGAGAG GGGCGTCCAG 
CTAACGAATC CCTTCTACAG CATCTGGGCC TCGGATGTTG GAACCGAGCT TGCTCTGTCG 
TTCATAATTT TGGCAGGAAT CTGCGCCTGT CTCTACTTCC TCTTCCTTTG TTTCATGGTG 
TTTCAAGTCT TCCGTAACAT CAGTGGGAAG AGGACATCCC TACCTGCCTT GTCTAAGGCC 
AGACGTCTCC ACTATGAGGG TCTCATCTTC AGGTTCAAAT TCCTCATGCT CCTCACTCTG 
TTCTGTGCTG CCATGACTGT CATCTTCTTC ATCATCAGCC AGGTTAACCA GGGTCACTGG 
CACTGGGGCG GACACACGGT GCAGGTGAAC AGTGCCTTCT TCACGGGCAT CTATGGCATG 
TGGAATCTGT ATGTCTTCGC CATCATGTTC CTCTACGCAC CTTCTCACAA ACATTATGGG 
GACGAACAGT CGAGTGGCCA GTGCATCACA GTTGATGAAG GCGTGAAGAG CGGCGAGAAC 
ATCCAGCTGA CCACCACCGT CACCCACGTG GACGGTCCCA CTGAGCTCTA CAGAATGATG 
GGCAAAGAGG CGCAGGAATA G