rlim cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rlim
Entrez Gene ID	107374918
Full Name	ring finger protein, LIM domain interacting
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015943211.1	XP_015798697.1	E3 ubiquitin-protein ligase RLIM

Pathways from BioSystems
Gene Ontology

The following rlim gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rlim cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo00354	XM_015943211.1 Latest version!	Nothobranchius furzeri ring finger protein, LIM domain interacting (rlim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rlim ( XM_015943211.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015798697.1 Nothobranchius furzeri ring finger protein, LIM domain interacting (rlim), mRNA.

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CloneID	ONo00354
Clone ID Related Accession (Same CDS sequence)	XM_015943211.1
Accession Version	XM_015943211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	E3 ubiquitin-protein ligase RLIM
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443480.1) and transcript sequence (GAIB01003417.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 151 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGAAGGGT CTGACAATGC TGAGCGAGGC GGCAGTGACC AGCCAGAGTC ACAACGCAGG 
AGGCAGCTGG ACCGCCTGGA TAGAGAGGAA GCTTTCTACC AGTTTGTCAA CAATCTCAGT 
GATGAGGACT ATCGTCTTAT GAGAGATAAC AACCTTTTAG GCAATCCAGG AGAGGTGACA 
GAAGATGAGC TATTTAGTAG ACTTCAACAA ATCAAAAATG GTCCAGAGCA GCAGAATAAC 
ACCACAAGCA CCGAAACTTC TGAAGAACCA GTTGAACAAC CAGAATCCTC TGAAGATGCT 
CCTGGGGGGG ATAGCCTCCT GGACTGGCTT AACACAGTGC GTCGCACAGG CAATACGACC 
AGGACTGGTC ATCGTGGAAA CCAGTCGTGG CGTGCCGTAA GCCAGACTAA CCCTAATAGT 
GGTGATTTTC GCTTCAGCTT GGAGATTAGT GTGAATCGAA ATCTGGCTGA ACAGCAAGCA 
GCTGCTGAAG GAGAACAGGA GCCCCCACCG CAAGCTCCTG AAGTTCCAGA AATGGTTGCA 
AATCCTGAAC CACAAACCCC CATGGAGACG GAGGTAGTGG AAGAACCAGT TGTAGAAGAA 
CTGGCTGTCA GTGTAGAGCC AGAACCTGAA CTAGAAGAAG CTGTTCCACA AGAAGTTGTT 
GGGGAGTCTC CCAGCCCACC TGCAGCCCCA CCAGAACAAG CACCACCTTG TTCTTCTCCT 
CGTAGAGGAC AAAGAAGAGC CCGCAGCCGT AGTCCAGAAC CTCGCAGGAC TAGGGCCCGT 
TTAGCCAGAA GCCGCTCCCC TTTCAATCTG GATCAACTTG ATGGTCTACC TAATCCACGA 
CATCCTTACA TCTCTCAAAG CCCAAGTTCT TCCTCAAACA CCCCCCCTGC ACCTCAAGTG 
GAGGGCAGTT CTCGGACTCG ACAGCACATC CTTACAAGGC AAAGCCCAGC TGATGCAGAT 
GCTCAGCCAC CTAGAGCCGA TCCAGAAGCA ATACCAGAGA TCCAACAAGT TGGATCGCAG 
GAAGGAGAGT CTCCTGGGAC TGAAGGAGGT GCACCAGGGC GTCGTCCCCC AACTATAATG 
CTCGATCTCC AGGTGCGCCG TGTGCGTCCA GGTGAATATC GGCAAAGGGA CAGCATCGCC 
AACCGTACCC GTTCCCGCTC CCAGAATTCA AACAACACAT ATCTTTTTGA GAGTGAACGC 
GGTGGTTTTC GTAGGACCTT CTCACGCTCT GAGCGTGCTG GTGTAAGGAC CTACGTCAGC 
ACAATTCGAA TCCCTATTCG AAGAATCTCA GATGCCGGTT TGGGAGAAGC AACCTCAATG 
GCTCTTCAGT CTATGATCAG GCAAATCATG ACAGGCTTTG GTGAGCTAGG CTACCTTATG 
GATTCAGACT CGGATTCTTC CGATTCAAAC CGCGGAACTG GCACACCCGC AGACTTGGAA 
ACCCTCAACA ATACAGAAGC AAACAACTTA GACACTTCTG GTTCTGCTGA TGTTGAAGAA 
CATCAACAAC AACCACCGGT GGCACCTGGG GTTAGAGGAC GGACAGTGGA GGCAGATGTG 
GATGAGGGCC TTGCCACCGG GCCACAGGGT TCAGGTAACA GGGCTCGACC TAGACCACCC 
ATTAGCCTAG AGGAGCCCAG CTCGCTGCCC TTCCTTCGAC TTGCTCATTT CTTCCTCCTA 
AATGATGATG ATGATGACCA ACCTCAGGGG CTGACTAAAG AACAGATTGA CAACCTCTCC 
ATGCGTAACT TTGGTGAGAG TGACGCCCTG AAGACCTGTA GTGTCTGTAT AACAGAGTAT 
TCCGAAGGGA ACAAACTACG TAAGCTCCCT TGCTCTCACG AGTACCACGT CCACTGCATA 
GACCGCTGGC TTTCTGAAAA CTCCACATGC CCCATCTGCC GCCGGGCTGT TCTGGTATCA 
GCCAACCGAG AGAGTGTGAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015798697.1
CDS	245..2188
Translation	MEGSDNAERGGSDQPESQRRRQLDRLDREEAFYQFVNNLSDEDYRLMRDNNLLGNPGEVTEDELFSRLQQIKNGPEQQNNTTSTETSEEPVEQPESSEDAPGGDSLLDWLNTVRRTGNTTRTGHRGNQSWRAVSQTNPNSGDFRFSLEISVNRNLAEQQAAAEGEQEPPPQAPEVPEMVANPEPQTPMETEVVEEPVVEELAVSVEPEPELEEAVPQEVVGESPSPPAAPPEQAPPCSSPRRGQRRARSRSPEPRRTRARLARSRSPFNLDQLDGLPNPRHPYISQSPSSSSNTPPAPQVEGSSRTRQHILTRQSPADADAQPPRADPEAIPEIQQVGSQEGESPGTEGGAPGRRPPTIMLDLQVRRVRPGEYRQRDSIANRTRSRSQNSNNTYLFESERGGFRRTFSRSERAGVRTYVSTIRIPIRRISDAGLGEATSMALQSMIRQIMTGFGELGYLMDSDSDSSDSNRGTGTPADLETLNNTEANNLDTSGSADVEEHQQQPPVAPGVRGRTVEADVDEGLATGPQGSGNRARPRPPISLEEPSSLPFLRLAHFFLLNDDDDDQPQGLTKEQIDNLSMRNFGESDALKTCSVCITEYSEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLVSANRESVI

Target ORF information:

RefSeq Version	XM_015943211.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri ring finger protein, LIM domain interacting (rlim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015943211.1

ORF Insert Sequence:

ATGGAAGGGT CTGACAATGC TGAGCGAGGC GGCAGTGACC AGCCAGAGTC ACAACGCAGG 
AGGCAGCTGG ACCGCCTGGA TAGAGAGGAA GCTTTCTACC AGTTTGTCAA CAATCTCAGT 
GATGAGGACT ATCGTCTTAT GAGAGATAAC AACCTTTTAG GCAATCCAGG AGAGGTGACA 
GAAGATGAGC TATTTAGTAG ACTTCAACAA ATCAAAAATG GTCCAGAGCA GCAGAATAAC 
ACCACAAGCA CCGAAACTTC TGAAGAACCA GTTGAACAAC CAGAATCCTC TGAAGATGCT 
CCTGGGGGGG ATAGCCTCCT GGACTGGCTT AACACAGTGC GTCGCACAGG CAATACGACC 
AGGACTGGTC ATCGTGGAAA CCAGTCGTGG CGTGCCGTAA GCCAGACTAA CCCTAATAGT 
GGTGATTTTC GCTTCAGCTT GGAGATTAGT GTGAATCGAA ATCTGGCTGA ACAGCAAGCA 
GCTGCTGAAG GAGAACAGGA GCCCCCACCG CAAGCTCCTG AAGTTCCAGA AATGGTTGCA 
AATCCTGAAC CACAAACCCC CATGGAGACG GAGGTAGTGG AAGAACCAGT TGTAGAAGAA 
CTGGCTGTCA GTGTAGAGCC AGAACCTGAA CTAGAAGAAG CTGTTCCACA AGAAGTTGTT 
GGGGAGTCTC CCAGCCCACC TGCAGCCCCA CCAGAACAAG CACCACCTTG TTCTTCTCCT 
CGTAGAGGAC AAAGAAGAGC CCGCAGCCGT AGTCCAGAAC CTCGCAGGAC TAGGGCCCGT 
TTAGCCAGAA GCCGCTCCCC TTTCAATCTG GATCAACTTG ATGGTCTACC TAATCCACGA 
CATCCTTACA TCTCTCAAAG CCCAAGTTCT TCCTCAAACA CCCCCCCTGC ACCTCAAGTG 
GAGGGCAGTT CTCGGACTCG ACAGCACATC CTTACAAGGC AAAGCCCAGC TGATGCAGAT 
GCTCAGCCAC CTAGAGCCGA TCCAGAAGCA ATACCAGAGA TCCAACAAGT TGGATCGCAG 
GAAGGAGAGT CTCCTGGGAC TGAAGGAGGT GCACCAGGGC GTCGTCCCCC AACTATAATG 
CTCGATCTCC AGGTGCGCCG TGTGCGTCCA GGTGAATATC GGCAAAGGGA CAGCATCGCC 
AACCGTACCC GTTCCCGCTC CCAGAATTCA AACAACACAT ATCTTTTTGA GAGTGAACGC 
GGTGGTTTTC GTAGGACCTT CTCACGCTCT GAGCGTGCTG GTGTAAGGAC CTACGTCAGC 
ACAATTCGAA TCCCTATTCG AAGAATCTCA GATGCCGGTT TGGGAGAAGC AACCTCAATG 
GCTCTTCAGT CTATGATCAG GCAAATCATG ACAGGCTTTG GTGAGCTAGG CTACCTTATG 
GATTCAGACT CGGATTCTTC CGATTCAAAC CGCGGAACTG GCACACCCGC AGACTTGGAA 
ACCCTCAACA ATACAGAAGC AAACAACTTA GACACTTCTG GTTCTGCTGA TGTTGAAGAA 
CATCAACAAC AACCACCGGT GGCACCTGGG GTTAGAGGAC GGACAGTGGA GGCAGATGTG 
GATGAGGGCC TTGCCACCGG GCCACAGGGT TCAGGTAACA GGGCTCGACC TAGACCACCC 
ATTAGCCTAG AGGAGCCCAG CTCGCTGCCC TTCCTTCGAC TTGCTCATTT CTTCCTCCTA 
AATGATGATG ATGATGACCA ACCTCAGGGG CTGACTAAAG AACAGATTGA CAACCTCTCC 
ATGCGTAACT TTGGTGAGAG TGACGCCCTG AAGACCTGTA GTGTCTGTAT AACAGAGTAT 
TCCGAAGGGA ACAAACTACG TAAGCTCCCT TGCTCTCACG AGTACCACGT CCACTGCATA 
GACCGCTGGC TTTCTGAAAA CTCCACATGC CCCATCTGCC GCCGGGCTGT TCTGGTATCA 
GCCAACCGAG AGAGTGTGAT ATAA