LOC104091585 cDNA ORF clone, Nicotiana tomentosiformis

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104091585
Entrez Gene ID	104091585
Full Name	putative pentatricopeptide repeat-containing protein At1g19290
Gene Type	protein-coding
Organism	Nicotiana tomentosiformis

mRNA and Protein(s)

mRNA	Protein	Name
XM_018769258.1	XP_018624774.1	putative pentatricopeptide repeat-containing protein At1g19290
XM_009596954.2	XP_009595249.2	putative pentatricopeptide repeat-containing protein At1g19290

Pathways from BioSystems
Gene Ontology

The following LOC104091585 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104091585 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONi90686	XM_018769258.1 Latest version!	Nicotiana tomentosiformis putative pentatricopeptide repeat-containing protein At1g19290 (LOC104091585), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ONi90686	XM_009596954.2 Latest version!	Nicotiana tomentosiformis putative pentatricopeptide repeat-containing protein At1g19290 (LOC104091585), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104091585 ( XM_018769258.1 ) cDNA ORF clone, Nicotiana tomentosiformis -> XP_018624774.1 Nicotiana tomentosiformis putative pentatricopeptide repeat-containing protein At1g19290 (LOC104091585), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONi90686
Clone ID Related Accession (Same CDS sequence)	XM_018769258.1 , XM_009596954.2
Accession Version	XM_018769258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Nicotiana tomentosiformis
Product	putative pentatricopeptide repeat-containing protein At1g19290
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008942040.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nicotiana tomentosiformis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAGAGAAAAT AGTAAACACA ATTCTCTCTA CTTCAAATAA AAAATTCACC 
TTTGGGCTAC TCCCTCAGCA ACGACGTAAC GCCACCCATT TTCCGGCGAC TAACCAACCA 
CCCAAACTCG CCGATCAAAT AAGCCGTCTT TTGATTCTCC AGCGTTACAC CGCCCTAGAC 
AACCTCAAAT TTGATTTCTC CGACAACCTC GTCGACGCCG TTCTCGTAAA ACTCAAACTC 
CATCCTAACG CTTCGTTGCA TTTCTTCAAG TTAGCTTCAC GGCGTCAGTT TTATAAACCC 
CATGTAAAAT CTTACTGCAA AATAGTTCAC ATATTATCCA GAGCGAGAAT GTTCGATGAG 
ACTAGGTTTT ATTTAGCCGA GCTTCTAGAA CTTTCTAGAA ACATAAAACC CGTGTCGTTT 
ATTTGGGATG AATTAGTAGC AGTTTATAGA GAGTTCAAGT TTTCTCCTAC GGTATTCGAT 
ATGGTGTTGA AAATTTCCGC GAAAAAGGGA TTAGTGAAAA ATGCCTTGTA CGTGTTTGAT 
AATATGCACA AGTGTGGTCG TGTTCCTAGT TTGAGCTCTT GTAATAGTTT ATTGAGTAAT 
TTGGTGAAAA ATGGTGAATG TTTTACTGCT TTTTCTGTAT ATGATCAGAT GAATAGGATT 
GGGATCTCTC CTGATATATA TATGTGTACG ATAATGGTAA ATGCATATTG CAAGGATGGG 
AAGGTTGATA AAGCTGAGAT TTTTATTGAT GAAATTGAGA AGATGGGTTT GGAGCTGAGT 
GTTGTTACTT ATCATAGTTT GATCAATGGG TATGTGGAGA AGAAGGATCT CGAGGGTGTC 
GAAAGAGTGT TGAAGCAAAT GGAGGAAAGA GGAATTTCGA GAAATGTAGT TACATTCACG 
TTGTTGATTA AGGGTTATTG CAGGCTGTGT AAGATGGAGG AAGCTGAGAA AGTGTTCAGA 
GAAATGAAGG AAGTGGATGA GCAGGCGTAT GGTGTGTTAA TTGACGGGTT TTGTCAAATA 
GGGAAAATGG ATGATGCCCT TAGGATTCGG GATGAGATGT TAAGATTAGG GTTTAACATG 
AACTTGTTCA TTTGTAATTC CTTGATAAAT GGGTATTGCA AAGTTGGAAA AATTAGCAAC 
GCGGAACAGG TTGTTAGGAG CATGAACGGC TGGAATCTAA AACCTGATTC GTATAGCTAT 
CACACCCTTT TAGATGGCTA TTGTAGGGAA AGATTGATGC AAAATGCATT CAACCTTTGT 
GATGAGATGA TTCGTGATGG TATCGATCCA ACAGTTGTCA CTTACAACAC TCTCCTGAAA 
GGACTATGTC AGGAAGGTGC CATTTCTGAT GCTTTGCATC TTTGGAATTT GATGCTTAAA 
AGAGGTGTTG TTCCTAATGC AGTTGGATAC AGTACTCTTT TAGATGCATT TCTTAAAATG 
GGAGAATTTG AAAAGGCTTT AGTCCTGTGG AAACATATAC TAGCAAGAGG GCACACCAAA 
AGCAGGATTC TTTTAAATAC GATGCTTAAA GGATTCTGTA AGATGGGGAA AATGGCTGAA 
GCTGAGCTGC TTTTTAACAA AATGGAGGAG CTTGGCTGTT CACCAGATGC AATAACTTAC 
AGGACACTAA GTGACGGGTT TTGTAAAACA GGGGAGATTG AAAAAGCTTT GGAAACAAAG 
GATGCCATGG AACTGCAAAA CATTCCTGCC TCTGTAGAAA GTTATAACTC CCTTATTAGT 
GGACTTATGA AGGCTGGGAA GTTTGGCAGA GTCGAAGATC TCCTTAATGA AATGCATGAC 
AGGGAATTAA CTCCCAACAC TGTTACATTT GGAGCCCTTA TTGCTGGTTG GTTTAAAGAA 
GGATTACCTG AGAAAGCTTT CAAAGCATAT TTTGAGATGA GAGAGAAGGG ATTCAATCCA 
AATGTCATCA TAGTTAGCTC TATTATTAGT GGATTATACA AACTTGGTAG GACTGATGAT 
GCTAATAAGC TACTGCAGAA AATACTGGAT GTAAATTTGT ACCCTGACCT TAGACGTTTT 
TACAGTTTTT CAAACTCTAG GACAGAGCTG CTAGATGTGC AGAAAGTTGC GGATTCCCTT 
GATGGAAGTG CTGCAAAATC TATTGTTCCC AATAATGTGC TGTATAATAT AGCTGTGGCA 
GCGTTATGCA AATCAGGGAA GATCGATGAT GCAAGAGATG TTGTAAATTA CCTCTCACTA 
AAAGGCTTTA CTCCTGATGA GTTTACCTAC TGCACCCTTA TCCATGGCAT TTCTTTGGCT 
GGTAAAGTGA ATGAAGCTTT TGATTTGCGC GATGAGATGC TAATAAAGGA TCTTGTTCCA 
AATATTGCAG TATACAATGC TCTTATAGAT GGCCTTTGTA AAGCAGGCAA CCATGAACGA 
GCATTGAGAC TTTTCAATAA GCTTCATTTG AAAGGGTTAG CTCCTAATGT TATAACTTAT 
AACACTCTGA TAGATGGATA CTATAAGATT GGTAAAACTA GTGAAGCCGT GCAACTGCTG 
AAGAGAATAA CAGAAGAAGA AATCCTTCAG TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018624774.1
CDS	186..2741
Translation	MAQREKIVNTILSTSNKKFTFGLLPQQRRNATHFPATNQPPKLADQISRLLILQRYTALDNLKFDFSDNLVDAVLVKLKLHPNASLHFFKLASRRQFYKPHVKSYCKIVHILSRARMFDETRFYLAELLELSRNIKPVSFIWDELVAVYREFKFSPTVFDMVLKISAKKGLVKNALYVFDNMHKCGRVPSLSSCNSLLSNLVKNGECFTAFSVYDQMNRIGISPDIYMCTIMVNAYCKDGKVDKAEIFIDEIEKMGLELSVVTYHSLINGYVEKKDLEGVERVLKQMEERGISRNVVTFTLLIKGYCRLCKMEEAEKVFREMKEVDEQAYGVLIDGFCQIGKMDDALRIRDEMLRLGFNMNLFICNSLINGYCKVGKISNAEQVVRSMNGWNLKPDSYSYHTLLDGYCRERLMQNAFNLCDEMIRDGIDPTVVTYNTLLKGLCQEGAISDALHLWNLMLKRGVVPNAVGYSTLLDAFLKMGEFEKALVLWKHILARGHTKSRILLNTMLKGFCKMGKMAEAELLFNKMEELGCSPDAITYRTLSDGFCKTGEIEKALETKDAMELQNIPASVESYNSLISGLMKAGKFGRVEDLLNEMHDRELTPNTVTFGALIAGWFKEGLPEKAFKAYFEMREKGFNPNVIIVSSIISGLYKLGRTDDANKLLQKILDVNLYPDLRRFYSFSNSRTELLDVQKVADSLDGSAAKSIVPNNVLYNIAVAALCKSGKIDDARDVVNYLSLKGFTPDEFTYCTLIHGISLAGKVNEAFDLRDEMLIKDLVPNIAVYNALIDGLCKAGNHERALRLFNKLHLKGLAPNVITYNTLIDGYYKIGKTSEAVQLLKRITEEEILQS

Target ORF information:

RefSeq Version	XM_018769258.1
Organism	Nicotiana tomentosiformis
Definition	Nicotiana tomentosiformis putative pentatricopeptide repeat-containing protein At1g19290 (LOC104091585), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018769258.1

ORF Insert Sequence:

ATGGCACAGA GAGAGAAAAT AGTAAACACA ATTCTCTCTA CTTCAAATAA AAAATTCACC 
TTTGGGCTAC TCCCTCAGCA ACGACGTAAC GCCACCCATT TTCCGGCGAC TAACCAACCA 
CCCAAACTCG CCGATCAAAT AAGCCGTCTT TTGATTCTCC AGCGTTACAC CGCCCTAGAC 
AACCTCAAAT TTGATTTCTC CGACAACCTC GTCGACGCCG TTCTCGTAAA ACTCAAACTC 
CATCCTAACG CTTCGTTGCA TTTCTTCAAG TTAGCTTCAC GGCGTCAGTT TTATAAACCC 
CATGTAAAAT CTTACTGCAA AATAGTTCAC ATATTATCCA GAGCGAGAAT GTTCGATGAG 
ACTAGGTTTT ATTTAGCCGA GCTTCTAGAA CTTTCTAGAA ACATAAAACC CGTGTCGTTT 
ATTTGGGATG AATTAGTAGC AGTTTATAGA GAGTTCAAGT TTTCTCCTAC GGTATTCGAT 
ATGGTGTTGA AAATTTCCGC GAAAAAGGGA TTAGTGAAAA ATGCCTTGTA CGTGTTTGAT 
AATATGCACA AGTGTGGTCG TGTTCCTAGT TTGAGCTCTT GTAATAGTTT ATTGAGTAAT 
TTGGTGAAAA ATGGTGAATG TTTTACTGCT TTTTCTGTAT ATGATCAGAT GAATAGGATT 
GGGATCTCTC CTGATATATA TATGTGTACG ATAATGGTAA ATGCATATTG CAAGGATGGG 
AAGGTTGATA AAGCTGAGAT TTTTATTGAT GAAATTGAGA AGATGGGTTT GGAGCTGAGT 
GTTGTTACTT ATCATAGTTT GATCAATGGG TATGTGGAGA AGAAGGATCT CGAGGGTGTC 
GAAAGAGTGT TGAAGCAAAT GGAGGAAAGA GGAATTTCGA GAAATGTAGT TACATTCACG 
TTGTTGATTA AGGGTTATTG CAGGCTGTGT AAGATGGAGG AAGCTGAGAA AGTGTTCAGA 
GAAATGAAGG AAGTGGATGA GCAGGCGTAT GGTGTGTTAA TTGACGGGTT TTGTCAAATA 
GGGAAAATGG ATGATGCCCT TAGGATTCGG GATGAGATGT TAAGATTAGG GTTTAACATG 
AACTTGTTCA TTTGTAATTC CTTGATAAAT GGGTATTGCA AAGTTGGAAA AATTAGCAAC 
GCGGAACAGG TTGTTAGGAG CATGAACGGC TGGAATCTAA AACCTGATTC GTATAGCTAT 
CACACCCTTT TAGATGGCTA TTGTAGGGAA AGATTGATGC AAAATGCATT CAACCTTTGT 
GATGAGATGA TTCGTGATGG TATCGATCCA ACAGTTGTCA CTTACAACAC TCTCCTGAAA 
GGACTATGTC AGGAAGGTGC CATTTCTGAT GCTTTGCATC TTTGGAATTT GATGCTTAAA 
AGAGGTGTTG TTCCTAATGC AGTTGGATAC AGTACTCTTT TAGATGCATT TCTTAAAATG 
GGAGAATTTG AAAAGGCTTT AGTCCTGTGG AAACATATAC TAGCAAGAGG GCACACCAAA 
AGCAGGATTC TTTTAAATAC GATGCTTAAA GGATTCTGTA AGATGGGGAA AATGGCTGAA 
GCTGAGCTGC TTTTTAACAA AATGGAGGAG CTTGGCTGTT CACCAGATGC AATAACTTAC 
AGGACACTAA GTGACGGGTT TTGTAAAACA GGGGAGATTG AAAAAGCTTT GGAAACAAAG 
GATGCCATGG AACTGCAAAA CATTCCTGCC TCTGTAGAAA GTTATAACTC CCTTATTAGT 
GGACTTATGA AGGCTGGGAA GTTTGGCAGA GTCGAAGATC TCCTTAATGA AATGCATGAC 
AGGGAATTAA CTCCCAACAC TGTTACATTT GGAGCCCTTA TTGCTGGTTG GTTTAAAGAA 
GGATTACCTG AGAAAGCTTT CAAAGCATAT TTTGAGATGA GAGAGAAGGG ATTCAATCCA 
AATGTCATCA TAGTTAGCTC TATTATTAGT GGATTATACA AACTTGGTAG GACTGATGAT 
GCTAATAAGC TACTGCAGAA AATACTGGAT GTAAATTTGT ACCCTGACCT TAGACGTTTT 
TACAGTTTTT CAAACTCTAG GACAGAGCTG CTAGATGTGC AGAAAGTTGC GGATTCCCTT 
GATGGAAGTG CTGCAAAATC TATTGTTCCC AATAATGTGC TGTATAATAT AGCTGTGGCA 
GCGTTATGCA AATCAGGGAA GATCGATGAT GCAAGAGATG TTGTAAATTA CCTCTCACTA 
AAAGGCTTTA CTCCTGATGA GTTTACCTAC TGCACCCTTA TCCATGGCAT TTCTTTGGCT 
GGTAAAGTGA ATGAAGCTTT TGATTTGCGC GATGAGATGC TAATAAAGGA TCTTGTTCCA 
AATATTGCAG TATACAATGC TCTTATAGAT GGCCTTTGTA AAGCAGGCAA CCATGAACGA 
GCATTGAGAC TTTTCAATAA GCTTCATTTG AAAGGGTTAG CTCCTAATGT TATAACTTAT 
AACACTCTGA TAGATGGATA CTATAAGATT GGTAAAACTA GTGAAGCCGT GCAACTGCTG 
AAGAGAATAA CAGAAGAAGA AATCCTTCAG TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104091585 ( XM_009596954.2 ) cDNA ORF clone, Nicotiana tomentosiformis -> XP_009595249.2 Nicotiana tomentosiformis putative pentatricopeptide repeat-containing protein At1g19290 (LOC104091585), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONi90686
Clone ID Related Accession (Same CDS sequence)	XM_018769258.1 , XM_009596954.2
Accession Version	XM_009596954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Nicotiana tomentosiformis
Product	putative pentatricopeptide repeat-containing protein At1g19290
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008942040.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 19, 2016 this sequence version replaced XM_009596954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nicotiana tomentosiformis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAGAGAAAAT AGTAAACACA ATTCTCTCTA CTTCAAATAA AAAATTCACC 
TTTGGGCTAC TCCCTCAGCA ACGACGTAAC GCCACCCATT TTCCGGCGAC TAACCAACCA 
CCCAAACTCG CCGATCAAAT AAGCCGTCTT TTGATTCTCC AGCGTTACAC CGCCCTAGAC 
AACCTCAAAT TTGATTTCTC CGACAACCTC GTCGACGCCG TTCTCGTAAA ACTCAAACTC 
CATCCTAACG CTTCGTTGCA TTTCTTCAAG TTAGCTTCAC GGCGTCAGTT TTATAAACCC 
CATGTAAAAT CTTACTGCAA AATAGTTCAC ATATTATCCA GAGCGAGAAT GTTCGATGAG 
ACTAGGTTTT ATTTAGCCGA GCTTCTAGAA CTTTCTAGAA ACATAAAACC CGTGTCGTTT 
ATTTGGGATG AATTAGTAGC AGTTTATAGA GAGTTCAAGT TTTCTCCTAC GGTATTCGAT 
ATGGTGTTGA AAATTTCCGC GAAAAAGGGA TTAGTGAAAA ATGCCTTGTA CGTGTTTGAT 
AATATGCACA AGTGTGGTCG TGTTCCTAGT TTGAGCTCTT GTAATAGTTT ATTGAGTAAT 
TTGGTGAAAA ATGGTGAATG TTTTACTGCT TTTTCTGTAT ATGATCAGAT GAATAGGATT 
GGGATCTCTC CTGATATATA TATGTGTACG ATAATGGTAA ATGCATATTG CAAGGATGGG 
AAGGTTGATA AAGCTGAGAT TTTTATTGAT GAAATTGAGA AGATGGGTTT GGAGCTGAGT 
GTTGTTACTT ATCATAGTTT GATCAATGGG TATGTGGAGA AGAAGGATCT CGAGGGTGTC 
GAAAGAGTGT TGAAGCAAAT GGAGGAAAGA GGAATTTCGA GAAATGTAGT TACATTCACG 
TTGTTGATTA AGGGTTATTG CAGGCTGTGT AAGATGGAGG AAGCTGAGAA AGTGTTCAGA 
GAAATGAAGG AAGTGGATGA GCAGGCGTAT GGTGTGTTAA TTGACGGGTT TTGTCAAATA 
GGGAAAATGG ATGATGCCCT TAGGATTCGG GATGAGATGT TAAGATTAGG GTTTAACATG 
AACTTGTTCA TTTGTAATTC CTTGATAAAT GGGTATTGCA AAGTTGGAAA AATTAGCAAC 
GCGGAACAGG TTGTTAGGAG CATGAACGGC TGGAATCTAA AACCTGATTC GTATAGCTAT 
CACACCCTTT TAGATGGCTA TTGTAGGGAA AGATTGATGC AAAATGCATT CAACCTTTGT 
GATGAGATGA TTCGTGATGG TATCGATCCA ACAGTTGTCA CTTACAACAC TCTCCTGAAA 
GGACTATGTC AGGAAGGTGC CATTTCTGAT GCTTTGCATC TTTGGAATTT GATGCTTAAA 
AGAGGTGTTG TTCCTAATGC AGTTGGATAC AGTACTCTTT TAGATGCATT TCTTAAAATG 
GGAGAATTTG AAAAGGCTTT AGTCCTGTGG AAACATATAC TAGCAAGAGG GCACACCAAA 
AGCAGGATTC TTTTAAATAC GATGCTTAAA GGATTCTGTA AGATGGGGAA AATGGCTGAA 
GCTGAGCTGC TTTTTAACAA AATGGAGGAG CTTGGCTGTT CACCAGATGC AATAACTTAC 
AGGACACTAA GTGACGGGTT TTGTAAAACA GGGGAGATTG AAAAAGCTTT GGAAACAAAG 
GATGCCATGG AACTGCAAAA CATTCCTGCC TCTGTAGAAA GTTATAACTC CCTTATTAGT 
GGACTTATGA AGGCTGGGAA GTTTGGCAGA GTCGAAGATC TCCTTAATGA AATGCATGAC 
AGGGAATTAA CTCCCAACAC TGTTACATTT GGAGCCCTTA TTGCTGGTTG GTTTAAAGAA 
GGATTACCTG AGAAAGCTTT CAAAGCATAT TTTGAGATGA GAGAGAAGGG ATTCAATCCA 
AATGTCATCA TAGTTAGCTC TATTATTAGT GGATTATACA AACTTGGTAG GACTGATGAT 
GCTAATAAGC TACTGCAGAA AATACTGGAT GTAAATTTGT ACCCTGACCT TAGACGTTTT 
TACAGTTTTT CAAACTCTAG GACAGAGCTG CTAGATGTGC AGAAAGTTGC GGATTCCCTT 
GATGGAAGTG CTGCAAAATC TATTGTTCCC AATAATGTGC TGTATAATAT AGCTGTGGCA 
GCGTTATGCA AATCAGGGAA GATCGATGAT GCAAGAGATG TTGTAAATTA CCTCTCACTA 
AAAGGCTTTA CTCCTGATGA GTTTACCTAC TGCACCCTTA TCCATGGCAT TTCTTTGGCT 
GGTAAAGTGA ATGAAGCTTT TGATTTGCGC GATGAGATGC TAATAAAGGA TCTTGTTCCA 
AATATTGCAG TATACAATGC TCTTATAGAT GGCCTTTGTA AAGCAGGCAA CCATGAACGA 
GCATTGAGAC TTTTCAATAA GCTTCATTTG AAAGGGTTAG CTCCTAATGT TATAACTTAT 
AACACTCTGA TAGATGGATA CTATAAGATT GGTAAAACTA GTGAAGCCGT GCAACTGCTG 
AAGAGAATAA CAGAAGAAGA AATCCTTCAG TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009595249.2
CDS	186..2741
Translation	MAQREKIVNTILSTSNKKFTFGLLPQQRRNATHFPATNQPPKLADQISRLLILQRYTALDNLKFDFSDNLVDAVLVKLKLHPNASLHFFKLASRRQFYKPHVKSYCKIVHILSRARMFDETRFYLAELLELSRNIKPVSFIWDELVAVYREFKFSPTVFDMVLKISAKKGLVKNALYVFDNMHKCGRVPSLSSCNSLLSNLVKNGECFTAFSVYDQMNRIGISPDIYMCTIMVNAYCKDGKVDKAEIFIDEIEKMGLELSVVTYHSLINGYVEKKDLEGVERVLKQMEERGISRNVVTFTLLIKGYCRLCKMEEAEKVFREMKEVDEQAYGVLIDGFCQIGKMDDALRIRDEMLRLGFNMNLFICNSLINGYCKVGKISNAEQVVRSMNGWNLKPDSYSYHTLLDGYCRERLMQNAFNLCDEMIRDGIDPTVVTYNTLLKGLCQEGAISDALHLWNLMLKRGVVPNAVGYSTLLDAFLKMGEFEKALVLWKHILARGHTKSRILLNTMLKGFCKMGKMAEAELLFNKMEELGCSPDAITYRTLSDGFCKTGEIEKALETKDAMELQNIPASVESYNSLISGLMKAGKFGRVEDLLNEMHDRELTPNTVTFGALIAGWFKEGLPEKAFKAYFEMREKGFNPNVIIVSSIISGLYKLGRTDDANKLLQKILDVNLYPDLRRFYSFSNSRTELLDVQKVADSLDGSAAKSIVPNNVLYNIAVAALCKSGKIDDARDVVNYLSLKGFTPDEFTYCTLIHGISLAGKVNEAFDLRDEMLIKDLVPNIAVYNALIDGLCKAGNHERALRLFNKLHLKGLAPNVITYNTLIDGYYKIGKTSEAVQLLKRITEEEILQS

Target ORF information:

RefSeq Version	XM_009596954.2
Organism	Nicotiana tomentosiformis
Definition	Nicotiana tomentosiformis putative pentatricopeptide repeat-containing protein At1g19290 (LOC104091585), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009596954.2

ORF Insert Sequence:

ATGGCACAGA GAGAGAAAAT AGTAAACACA ATTCTCTCTA CTTCAAATAA AAAATTCACC 
TTTGGGCTAC TCCCTCAGCA ACGACGTAAC GCCACCCATT TTCCGGCGAC TAACCAACCA 
CCCAAACTCG CCGATCAAAT AAGCCGTCTT TTGATTCTCC AGCGTTACAC CGCCCTAGAC 
AACCTCAAAT TTGATTTCTC CGACAACCTC GTCGACGCCG TTCTCGTAAA ACTCAAACTC 
CATCCTAACG CTTCGTTGCA TTTCTTCAAG TTAGCTTCAC GGCGTCAGTT TTATAAACCC 
CATGTAAAAT CTTACTGCAA AATAGTTCAC ATATTATCCA GAGCGAGAAT GTTCGATGAG 
ACTAGGTTTT ATTTAGCCGA GCTTCTAGAA CTTTCTAGAA ACATAAAACC CGTGTCGTTT 
ATTTGGGATG AATTAGTAGC AGTTTATAGA GAGTTCAAGT TTTCTCCTAC GGTATTCGAT 
ATGGTGTTGA AAATTTCCGC GAAAAAGGGA TTAGTGAAAA ATGCCTTGTA CGTGTTTGAT 
AATATGCACA AGTGTGGTCG TGTTCCTAGT TTGAGCTCTT GTAATAGTTT ATTGAGTAAT 
TTGGTGAAAA ATGGTGAATG TTTTACTGCT TTTTCTGTAT ATGATCAGAT GAATAGGATT 
GGGATCTCTC CTGATATATA TATGTGTACG ATAATGGTAA ATGCATATTG CAAGGATGGG 
AAGGTTGATA AAGCTGAGAT TTTTATTGAT GAAATTGAGA AGATGGGTTT GGAGCTGAGT 
GTTGTTACTT ATCATAGTTT GATCAATGGG TATGTGGAGA AGAAGGATCT CGAGGGTGTC 
GAAAGAGTGT TGAAGCAAAT GGAGGAAAGA GGAATTTCGA GAAATGTAGT TACATTCACG 
TTGTTGATTA AGGGTTATTG CAGGCTGTGT AAGATGGAGG AAGCTGAGAA AGTGTTCAGA 
GAAATGAAGG AAGTGGATGA GCAGGCGTAT GGTGTGTTAA TTGACGGGTT TTGTCAAATA 
GGGAAAATGG ATGATGCCCT TAGGATTCGG GATGAGATGT TAAGATTAGG GTTTAACATG 
AACTTGTTCA TTTGTAATTC CTTGATAAAT GGGTATTGCA AAGTTGGAAA AATTAGCAAC 
GCGGAACAGG TTGTTAGGAG CATGAACGGC TGGAATCTAA AACCTGATTC GTATAGCTAT 
CACACCCTTT TAGATGGCTA TTGTAGGGAA AGATTGATGC AAAATGCATT CAACCTTTGT 
GATGAGATGA TTCGTGATGG TATCGATCCA ACAGTTGTCA CTTACAACAC TCTCCTGAAA 
GGACTATGTC AGGAAGGTGC CATTTCTGAT GCTTTGCATC TTTGGAATTT GATGCTTAAA 
AGAGGTGTTG TTCCTAATGC AGTTGGATAC AGTACTCTTT TAGATGCATT TCTTAAAATG 
GGAGAATTTG AAAAGGCTTT AGTCCTGTGG AAACATATAC TAGCAAGAGG GCACACCAAA 
AGCAGGATTC TTTTAAATAC GATGCTTAAA GGATTCTGTA AGATGGGGAA AATGGCTGAA 
GCTGAGCTGC TTTTTAACAA AATGGAGGAG CTTGGCTGTT CACCAGATGC AATAACTTAC 
AGGACACTAA GTGACGGGTT TTGTAAAACA GGGGAGATTG AAAAAGCTTT GGAAACAAAG 
GATGCCATGG AACTGCAAAA CATTCCTGCC TCTGTAGAAA GTTATAACTC CCTTATTAGT 
GGACTTATGA AGGCTGGGAA GTTTGGCAGA GTCGAAGATC TCCTTAATGA AATGCATGAC 
AGGGAATTAA CTCCCAACAC TGTTACATTT GGAGCCCTTA TTGCTGGTTG GTTTAAAGAA 
GGATTACCTG AGAAAGCTTT CAAAGCATAT TTTGAGATGA GAGAGAAGGG ATTCAATCCA 
AATGTCATCA TAGTTAGCTC TATTATTAGT GGATTATACA AACTTGGTAG GACTGATGAT 
GCTAATAAGC TACTGCAGAA AATACTGGAT GTAAATTTGT ACCCTGACCT TAGACGTTTT 
TACAGTTTTT CAAACTCTAG GACAGAGCTG CTAGATGTGC AGAAAGTTGC GGATTCCCTT 
GATGGAAGTG CTGCAAAATC TATTGTTCCC AATAATGTGC TGTATAATAT AGCTGTGGCA 
GCGTTATGCA AATCAGGGAA GATCGATGAT GCAAGAGATG TTGTAAATTA CCTCTCACTA 
AAAGGCTTTA CTCCTGATGA GTTTACCTAC TGCACCCTTA TCCATGGCAT TTCTTTGGCT 
GGTAAAGTGA ATGAAGCTTT TGATTTGCGC GATGAGATGC TAATAAAGGA TCTTGTTCCA 
AATATTGCAG TATACAATGC TCTTATAGAT GGCCTTTGTA AAGCAGGCAA CCATGAACGA 
GCATTGAGAC TTTTCAATAA GCTTCATTTG AAAGGGTTAG CTCCTAATGT TATAACTTAT 
AACACTCTGA TAGATGGATA CTATAAGATT GGTAAAACTA GTGAAGCCGT GCAACTGCTG 
AAGAGAATAA CAGAAGAAGA AATCCTTCAG TCATAA