cel-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	cel-1
Entrez Gene ID	3878614
Full Name	chain elongation-1
Synonyms	fas,ol
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395935.1	XP_011394237.1	fatty acid synthase alpha subunit reductase
XM_011395936.1	XP_011394238.1	fatty acid synthase alpha subunit reductase, variant

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	FAS2	NP_015093.1
Kluyveromyces lactis	KLLA0C15983g	XP_452914.1
Neurospora crassa	NCU07308	XP_962466.1
Eremothecium gossypii	AGOS_AFL138W	NP_985412.2
Schizosaccharomyces pombe (fission yeast)	fas2	NP_593823.1
Magnaporthe oryzae (rice blast fungus)	MGG_12154	XP_003719713.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cel-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cel-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf01153	XM_011395935.1 Latest version!	Neurospora crassa OR74A fatty acid synthase alpha subunit reductase (cel-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf01153	XM_011395936.1 Latest version!	Neurospora crassa OR74A fatty acid synthase alpha subunit reductase, variant (cel-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cel-1 ( XM_011395935.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394237.1 Neurospora crassa OR74A fatty acid synthase alpha subunit reductase (cel-1), mRNA.

Price & Availability↑

CloneID	ONf01153
Clone ID Related Accession (Same CDS sequence)	XM_011395935.1 , XM_011395936.1
Accession Version	XM_011395935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	fatty acid synthase alpha subunit reductase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGCGTCCCG AACTTGAGCA AGAGCTCGCC CACACCCTAC TCGTCGAGCT CCTTGCTTAC 
CAATTCGCCT CTCCCGTGAG ATGGATTGAG ACGCAAGATG TCTTCCTCGC GGAGAAGACA 
GCCGAGCGGG TTGTCGAGAT TGGTCCCGCC GACACCCTCG GTGGTATGGC CAGGAGGACG 
CTCGCCTCCA AGTACGAAGC ATTCGACGCT GCCAAGTCAA TCCAGAGACA GATTCTTTGC 
TACAACAAGG ACGCAAAGGA GATTTACTAC GATGTAGACC CTGTGGAAGA AGAGCCTGAG 
CCGGTTGCGG CTAGCTCATC ATCTGCTGCT CCCACCCCCG CTGCCGCTGC TGCTCCGGCT 
GCTGCCGCTG CTGCTCCTCC GCCCCCGAGT GCTGGCCCCG CGGCTTCAGT TCCCGATGCC 
CCAGTTCCCG CTCTTGACAT TGTCAGGTCG TTGATCGCCC AGAAGCTCAA GAAGCCCTTT 
GCAGAGATTC CTTTGAGCAA GGCTATCAAG GACTTGGTAG GAGGCAAGTC CACTCTACAA 
AACGAAATCC TTGGTGATCT AGGCAAAGAG TTCGGATCCA CACCGGAGAA GCCTGAAGAT 
ACGCCCCTCG ATGAGCTGGG TGCTGCTATG CAGGCTACCT TCGATGGTAA CCTAGGCAAG 
ACAACCCAGG GTCTTATCGC TCGTCTTATT TCGTCCAAGA TGCCCGGTGG CTTCAACATC 
ACATCAGCAC GCAAATATCT CGAGACAAGG TGGGGTCTCG GTTCTGGCCG TCAGGACGGT 
GCGCTTCTGC TCGCCATCAC TATGGAGCCT CCAGCTCGTC TTGGCTCCGA GGCCGAAGCG 
AAGGCTTATC TAGATGATAT TTCTCAAAAG TATGCCTCGC ATGCTGGCAT CAGCTTGTCC 
ACCGCGGCCG CGGCTGGCCC TGCTGCCGGA GGCGGCGGCG GCATGATGAT GGATCCTGCA 
GCCATTGAGG CCCTTACCAG CGACCAGAAG GCTATGTTCA AGCAACAATT GGAGCTTATC 
GCCAGATATC TCAAGATCGA TATCCGCGCC GGAGACAAGG CTTTCCAGGC GTCTCAGGAA 
TCTTCCAAGG TCCTGCAGTC ACAGCTGGAT CTCTGGATGG CCGAGCACGG CGATTTCTAC 
GCTGCCGGCA TTGAACCTGT CTTTAGCTCC CTCAAAGCTC GTGTGTACGA TTCTTCCTGG 
AACTGGGCTC GCCAGGATGC TCTCAGCATG TACTATGATA TCATCTTCGG CAGGCTGAAG 
GCCGTTGATC GCGAGATTGT CAGCCAGTGC ATCCGCATCA TGAACCGCTC TAATCCCACC 
CTCCTCGAGT TCATGCAATA CCACATCGAC AACTGCCCTA CGGAGCGCGG AGAGACGTAT 
CAGCTTGCCA AGGAGTTGGG CTATCAGCTC ATTGAGAACT GTAAGGAGGT TCTCAACGTT 
GACCCTGTCT ACAAGGATGT TGCCGTCCCT ACCGGCCCCC GTACCACCAT TGACGCTCGT 
GGCAACATCA AGTACGAGGA GATTCCTCGT GCAAGCTGCC GCAAGCTTGA GCATTACGTA 
CAGCAGATGG CCGAGGGTGG CAAGATCTCT GAATACAGCA ACCGTACCAA GGTTGAGAAT 
GATCTTGCTC GCATTTACAA GCTGATTAAG CAGCAGCACA AGCTCTCCAA GAACTCTCAG 
CTCGAGATCA AGTCTCTGTA TGGCGACATC ATCAGGTCTC TGAGCATGAA CGAAAGCCAG 
ATTATGCCCA AGGAGTCCAG CAAGGTCAGC AGCGTCTTGT CCAAGTCGTC GTCCAAGTCC 
AAGGGCAAGA CCGAGACAAT TCCCTTCCTC CACCTGCGCA AGAAGTCCGA GCACGGCTGG 
GATTACAGCA AGAAGCTCAC CGGCCTCTAC CTTGACTGCT TGGAGCAGGC GGCCAAGAAT 
GGTGTTACCT TCGCCAACAA GTACGTGCTC ATGACTGGTG CGGGCGCCGG TTCCATCGGT 
GCCGAGGTTC TCCAGGGTCT TATCAGCGGT GGTGCCAAGG TCATCGTCAC GACCAGCCGT 
TTCTCGCGTG AAGTTACCGA GTACTACCAG TCTATGTATG CTAGATTTGG CTCTCGTGGC 
TCTCAGCTCG TTGTGGTTCC CTTTAACCAG GGTAGCGTGC AGGACATCAA CGCCTTGGTC 
GACTACATCT ACGACACCAA GACTGGTCTC GGTTGGGATC TTGACTACAT TGTTCCCTTC 
GCCGCCATCT CTGAGCAGGG TCGCCAGATT GATGGCATTG ACTCCAAGTC CGAGTTGGCT 
CACCGCATCA TGTTGACCAA CCTCATCCGC CTCCTTGGCA GCGTCAAGGC TCAGAAGGCC 
AGCCGCGGTT TCGAAACTCG TCCCGCCCAG GTCATCCTCC CGCTCTCTCC CAACCACGGT 
ACCTTTGGTA GCGATGGTCT CTACTCCGAG TCCAAGCTCG GCCTCGAGAC TCTCTTCAAC 
AGGTGGCATG CTGAGGACTG GGCCAACTAC CTCACTATCT GTGGTGCCAT CATTGGTTGG 
ACTCGTGGTA CCGGCCTCAT GTCTGGCAAC AACCTGGTTG CTGAGGGTGT TGAGAACTTT 
GGTGTCCGCA CCTTCTCCCA ACAGGAGATG GCCTTTAACT TGCTTGGCCT CATGTCGCCT 
ACTATTGTCG ACTTGTGCCA GAACGAGCCT GTCTTCGCTG ATCTGAACGG CGGTCTTCAG 
TTCATCACCA ACTTGAACGA GACCATGACT AGTCAGCGCA AGAACCTTAT GGAGACCAGC 
GATATTCGCA AGGCCGTCAC CAAGGAGACT GCTATTGAGA ACAAGATCGT CAATGGCGAG 
GACTCCGAGG CTCTGTACAA GAAGAAGGTC ATCGAGCCCC GTGCCAACAT CAAGTTTGAC 
TTCCCGACCT TGCCTGACTG GAAGTCCGAG GTTGCTCCTC TTAACGACAC TCTTAAGGGT 
ATGGTTGATC TGGAGAAGGT CGTTGTTGTC ACTGGTTTCG CTGAGGTTGG CCCCTGGGGT 
AACTCCAGAA CCCGCTGGGA GATGGAGGCC TATGGTGAGT TCTCACTTGA GGGCTGCATT 
GAGATGGCCT GGATCATGGG TCTCATCAAG AACCATAACG GTCCTATTAA GGGCAAGCCC 
TACTCTGGCT GGGTTGATGC CAAGACTGGC GAGCCCGTTG ATGACAAGGA CATCAAGCCC 
AAGTACGAGA AGTACATCCT TGAACACGCT GGTATCCGTC TGATCGAGCC CGAGCTATTC 
GACGGTTACG ATCCCAACAA GAAGCAGCTT CTCCATGAGG TTGTCATTGA GGAGGATCTC 
GATCCTTTCG AGGCCACCAA GGACACCGCT GAGGAGTTTA AGAGAGAGCA TGGCGATAAG 
GTCGACATCT TTGAGATTCC CGAAACTGGT CAGTACACTG TCCGTCTGAA GAAGGGTGCC 
TCGCTCTGGA TCCCCAAGGC TCTGCGCTTT GATCGTCTGG TTGCTGGCCA GATCCCCACT 
GGCTGGGACG CCAAGCGTTA CGGTATCCCC GACGACATCA TTTCTCAGGT GGATCCCGTC 
GCTCTCTTCG TCCTTGTGTC TACTGTGGAG GCTCTTCTCT CGTCTGGTAT CACCGACCCG 
TACGAGTTCT ACAAGTACGT CCATGTCTCC GAAGTGGGTA ACTGCGTTGG TTCTGGTATG 
GGTGGTGCAA CTGCACTCCG TAACATGCAC CGCGACAGAT ACCTTGATAA GCCTGTCCAG 
AACGATATTC TTCAAGAGTC CTTCATCAAC ACCATGGCCG CGTGGGTAAA CATGCTCTTG 
ATCTCGTCTT CTGGACCCAT CAAGACGCCG GTCGGTGCTT GCGCCACCGC TGTCGAGTCT 
ATCGACATTG GCTACGAGAC CATTATGGAG GGCAAGGCCC GCGTGTGCTT CGTTGGTGGC 
TTCGATGACT TTGGCGAGGA GGGCTCGTAT GAGTTCGCCA ACATGAAGGC TACCAGCAAC 
GCTGTTGATG AGTTTGCTCA TGGTCGCACG CCCAAGGAGA TGTCCAGGCC CACTACCACC 
ACGAGAAATG GCTTCATGGA GTCGCAGGGT AGCGGTGTTC AGGTCATCAT GACTGCCAAG 
CTTGCTTTGG ACATGGGCGT CCCCATCTAT GGTGTCCTTG CTTTGACCAC CACCGCTTCC 
GACAAGATTG GTCGCTCCGT CCCTGCGCCC GGCAAGGGTG TTCTTACTAC TGCTCGCGAG 
CGCCAGGGTA GCCTCCCCTC GCCCCTGCTC GATATCAAGT ACAGACGTCG CCAGATTGAG 
CGCCGGAAGA AGCAGCTTGA GAACGAGAAG GAGGAAGAGC TTGAGTATCT CGCCGATGAG 
ATCGAGGCCC TCAAGGCCGA AGGCAGGCCG CAGAAGGAGA TTGACGACTA TGCCCGCCAC 
AGAACCCAAC ACATTGAAGA GGAGGCCGAA AGACAGGTCA AGGAAGTTCT CCGCAGCTAC 
GGCAACAACT TCTGGAAGGG TGATGCTACG ATTGCTCCCC TTCGTGGTGC CCTCGCTACT 
TGGGGCCTCA CTATTGACGA TCTTGACGTC GCCTCTTTCC ACGGTACTTC CACCAAGGCC 
AACGACAAGA ATGAGTCGTC TGTCATCTGC CAGCAGCTCC GTCATCTTGG CCGCAAGAAG 
GGCAATGCCG TGCTCGGCAT CTTCCAGAAG TACCTCACTG GTCATCCCAA GGGTGCTGCT 
GGTGCCTGGA TGATGAACGG CTGCTTGCAA GTGCTCAACA GTGGCCTCGT CCCCGGCAAC 
CGCAATGCCG ACAACGTCGA CGCTGTCATG GAGGAGTTTG ACTACATTGT TTACCCTAGC 
AAGAGTATCC AGACTGATGG TGTCAAGGCC TTCTCCGTTA CATCGTTCGG TTTCGGCCAG 
AAGGGTGCTC AGGCCATTGG TGTCCATCCC AAGTATCTTT ATGCCACTCT TGATGAGGAG 
GTATTCAACG CTTACCGCAC CAAGGTGGAG GCTCGCCAGA AGAAGACCTA CCGCTTCTTC 
CACAACGGCC TTATCAACAA CACCCTGTTC GTTGCCAAGG ATAAATCTCC CTACTCTGAT 
GATCAGCTTC AATCGGTTCT CCTTAACCCC AAGGCCCGCG TCACCGAGGA CAAGAAGACC 
GGCCAGCTCA CGTATCCCGC CAACTTCATG GAGTTGGAGC CAGCACAGAA GCTGCAGAAG 
AAGAGCGAGG AATCTCTTGA GTCGACTGTT GCCCGTGCTG CAAACCGTCT CAAGAACAGC 
AACACCCGTG TTGGCTTCGA TCTTGAGGAG ATTTCCTCCA TCAACATCGA CAATGAAACC 
TTCTTGGAAC GCAACTTCAC CGAGGCTGAG CTCCAGTACT GCAAGGCTCC TAGCACTGGT 
CGGTCGCCGC AGAAGTCGTT TGCTGGTCGC TGGAGCGCGA AGGAGGCCGT CTTTAAGTCC 
CTTGGTGTCA CCGGCCAGGG TGCTGGTGCA TCCCTGAAGG ACATTGAGAT TCTCTCAGAT 
AGCAACGGGG GTCCCGTTGT GAAGTTGCAT GGTGCTGCTT TGGAGGCCGC GAATAAGGCT 
GGCGTTTCCA AGGTCAACGT GACGCTGTCC TATTCTGACA GCCATGCTGT TGCCATTGCT 
ACGGCTCACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394237.1
CDS	406..6000
Translation	MRPELEQELAHTLLVELLAYQFASPVRWIETQDVFLAEKTAERVVEIGPADTLGGMARRTLASKYEAFDAAKSIQRQILCYNKDAKEIYYDVDPVEEEPEPVAASSSSAAPTPAAAAAPAAAAAAPPPPSAGPAASVPDAPVPALDIVRSLIAQKLKKPFAEIPLSKAIKDLVGGKSTLQNEILGDLGKEFGSTPEKPEDTPLDELGAAMQATFDGNLGKTTQGLIARLISSKMPGGFNITSARKYLETRWGLGSGRQDGALLLAITMEPPARLGSEAEAKAYLDDISQKYASHAGISLSTAAAAGPAAGGGGGMMMDPAAIEALTSDQKAMFKQQLELIARYLKIDIRAGDKAFQASQESSKVLQSQLDLWMAEHGDFYAAGIEPVFSSLKARVYDSSWNWARQDALSMYYDIIFGRLKAVDREIVSQCIRIMNRSNPTLLEFMQYHIDNCPTERGETYQLAKELGYQLIENCKEVLNVDPVYKDVAVPTGPRTTIDARGNIKYEEIPRASCRKLEHYVQQMAEGGKISEYSNRTKVENDLARIYKLIKQQHKLSKNSQLEIKSLYGDIIRSLSMNESQIMPKESSKVSSVLSKSSSKSKGKTETIPFLHLRKKSEHGWDYSKKLTGLYLDCLEQAAKNGVTFANKYVLMTGAGAGSIGAEVLQGLISGGAKVIVTTSRFSREVTEYYQSMYARFGSRGSQLVVVPFNQGSVQDINALVDYIYDTKTGLGWDLDYIVPFAAISEQGRQIDGIDSKSELAHRIMLTNLIRLLGSVKAQKASRGFETRPAQVILPLSPNHGTFGSDGLYSESKLGLETLFNRWHAEDWANYLTICGAIIGWTRGTGLMSGNNLVAEGVENFGVRTFSQQEMAFNLLGLMSPTIVDLCQNEPVFADLNGGLQFITNLNETMTSQRKNLMETSDIRKAVTKETAIENKIVNGEDSEALYKKKVIEPRANIKFDFPTLPDWKSEVAPLNDTLKGMVDLEKVVVVTGFAEVGPWGNSRTRWEMEAYGEFSLEGCIEMAWIMGLIKNHNGPIKGKPYSGWVDAKTGEPVDDKDIKPKYEKYILEHAGIRLIEPELFDGYDPNKKQLLHEVVIEEDLDPFEATKDTAEEFKREHGDKVDIFEIPETGQYTVRLKKGASLWIPKALRFDRLVAGQIPTGWDAKRYGIPDDIISQVDPVALFVLVSTVEALLSSGITDPYEFYKYVHVSEVGNCVGSGMGGATALRNMHRDRYLDKPVQNDILQESFINTMAAWVNMLLISSSGPIKTPVGACATAVESIDIGYETIMEGKARVCFVGGFDDFGEEGSYEFANMKATSNAVDEFAHGRTPKEMSRPTTTTRNGFMESQGSGVQVIMTAKLALDMGVPIYGVLALTTTASDKIGRSVPAPGKGVLTTARERQGSLPSPLLDIKYRRRQIERRKKQLENEKEEELEYLADEIEALKAEGRPQKEIDDYARHRTQHIEEEAERQVKEVLRSYGNNFWKGDATIAPLRGALATWGLTIDDLDVASFHGTSTKANDKNESSVICQQLRHLGRKKGNAVLGIFQKYLTGHPKGAAGAWMMNGCLQVLNSGLVPGNRNADNVDAVMEEFDYIVYPSKSIQTDGVKAFSVTSFGFGQKGAQAIGVHPKYLYATLDEEVFNAYRTKVEARQKKTYRFFHNGLINNTLFVAKDKSPYSDDQLQSVLLNPKARVTEDKKTGQLTYPANFMELEPAQKLQKKSEESLESTVARAANRLKNSNTRVGFDLEEISSINIDNETFLERNFTEAELQYCKAPSTGRSPQKSFAGRWSAKEAVFKSLGVTGQGAGASLKDIEILSDSNGGPVVKLHGAALEAANKAGVSKVNVTLSYSDSHAVAIATAHF

Target ORF information:

RefSeq Version	XM_011395935.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A fatty acid synthase alpha subunit reductase (cel-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395935.1

ORF Insert Sequence:

ATGCGTCCCG AACTTGAGCA AGAGCTCGCC CACACCCTAC TCGTCGAGCT CCTTGCTTAC 
CAATTCGCCT CTCCCGTGAG ATGGATTGAG ACGCAAGATG TCTTCCTCGC GGAGAAGACA 
GCCGAGCGGG TTGTCGAGAT TGGTCCCGCC GACACCCTCG GTGGTATGGC CAGGAGGACG 
CTCGCCTCCA AGTACGAAGC ATTCGACGCT GCCAAGTCAA TCCAGAGACA GATTCTTTGC 
TACAACAAGG ACGCAAAGGA GATTTACTAC GATGTAGACC CTGTGGAAGA AGAGCCTGAG 
CCGGTTGCGG CTAGCTCATC ATCTGCTGCT CCCACCCCCG CTGCCGCTGC TGCTCCGGCT 
GCTGCCGCTG CTGCTCCTCC GCCCCCGAGT GCTGGCCCCG CGGCTTCAGT TCCCGATGCC 
CCAGTTCCCG CTCTTGACAT TGTCAGGTCG TTGATCGCCC AGAAGCTCAA GAAGCCCTTT 
GCAGAGATTC CTTTGAGCAA GGCTATCAAG GACTTGGTAG GAGGCAAGTC CACTCTACAA 
AACGAAATCC TTGGTGATCT AGGCAAAGAG TTCGGATCCA CACCGGAGAA GCCTGAAGAT 
ACGCCCCTCG ATGAGCTGGG TGCTGCTATG CAGGCTACCT TCGATGGTAA CCTAGGCAAG 
ACAACCCAGG GTCTTATCGC TCGTCTTATT TCGTCCAAGA TGCCCGGTGG CTTCAACATC 
ACATCAGCAC GCAAATATCT CGAGACAAGG TGGGGTCTCG GTTCTGGCCG TCAGGACGGT 
GCGCTTCTGC TCGCCATCAC TATGGAGCCT CCAGCTCGTC TTGGCTCCGA GGCCGAAGCG 
AAGGCTTATC TAGATGATAT TTCTCAAAAG TATGCCTCGC ATGCTGGCAT CAGCTTGTCC 
ACCGCGGCCG CGGCTGGCCC TGCTGCCGGA GGCGGCGGCG GCATGATGAT GGATCCTGCA 
GCCATTGAGG CCCTTACCAG CGACCAGAAG GCTATGTTCA AGCAACAATT GGAGCTTATC 
GCCAGATATC TCAAGATCGA TATCCGCGCC GGAGACAAGG CTTTCCAGGC GTCTCAGGAA 
TCTTCCAAGG TCCTGCAGTC ACAGCTGGAT CTCTGGATGG CCGAGCACGG CGATTTCTAC 
GCTGCCGGCA TTGAACCTGT CTTTAGCTCC CTCAAAGCTC GTGTGTACGA TTCTTCCTGG 
AACTGGGCTC GCCAGGATGC TCTCAGCATG TACTATGATA TCATCTTCGG CAGGCTGAAG 
GCCGTTGATC GCGAGATTGT CAGCCAGTGC ATCCGCATCA TGAACCGCTC TAATCCCACC 
CTCCTCGAGT TCATGCAATA CCACATCGAC AACTGCCCTA CGGAGCGCGG AGAGACGTAT 
CAGCTTGCCA AGGAGTTGGG CTATCAGCTC ATTGAGAACT GTAAGGAGGT TCTCAACGTT 
GACCCTGTCT ACAAGGATGT TGCCGTCCCT ACCGGCCCCC GTACCACCAT TGACGCTCGT 
GGCAACATCA AGTACGAGGA GATTCCTCGT GCAAGCTGCC GCAAGCTTGA GCATTACGTA 
CAGCAGATGG CCGAGGGTGG CAAGATCTCT GAATACAGCA ACCGTACCAA GGTTGAGAAT 
GATCTTGCTC GCATTTACAA GCTGATTAAG CAGCAGCACA AGCTCTCCAA GAACTCTCAG 
CTCGAGATCA AGTCTCTGTA TGGCGACATC ATCAGGTCTC TGAGCATGAA CGAAAGCCAG 
ATTATGCCCA AGGAGTCCAG CAAGGTCAGC AGCGTCTTGT CCAAGTCGTC GTCCAAGTCC 
AAGGGCAAGA CCGAGACAAT TCCCTTCCTC CACCTGCGCA AGAAGTCCGA GCACGGCTGG 
GATTACAGCA AGAAGCTCAC CGGCCTCTAC CTTGACTGCT TGGAGCAGGC GGCCAAGAAT 
GGTGTTACCT TCGCCAACAA GTACGTGCTC ATGACTGGTG CGGGCGCCGG TTCCATCGGT 
GCCGAGGTTC TCCAGGGTCT TATCAGCGGT GGTGCCAAGG TCATCGTCAC GACCAGCCGT 
TTCTCGCGTG AAGTTACCGA GTACTACCAG TCTATGTATG CTAGATTTGG CTCTCGTGGC 
TCTCAGCTCG TTGTGGTTCC CTTTAACCAG GGTAGCGTGC AGGACATCAA CGCCTTGGTC 
GACTACATCT ACGACACCAA GACTGGTCTC GGTTGGGATC TTGACTACAT TGTTCCCTTC 
GCCGCCATCT CTGAGCAGGG TCGCCAGATT GATGGCATTG ACTCCAAGTC CGAGTTGGCT 
CACCGCATCA TGTTGACCAA CCTCATCCGC CTCCTTGGCA GCGTCAAGGC TCAGAAGGCC 
AGCCGCGGTT TCGAAACTCG TCCCGCCCAG GTCATCCTCC CGCTCTCTCC CAACCACGGT 
ACCTTTGGTA GCGATGGTCT CTACTCCGAG TCCAAGCTCG GCCTCGAGAC TCTCTTCAAC 
AGGTGGCATG CTGAGGACTG GGCCAACTAC CTCACTATCT GTGGTGCCAT CATTGGTTGG 
ACTCGTGGTA CCGGCCTCAT GTCTGGCAAC AACCTGGTTG CTGAGGGTGT TGAGAACTTT 
GGTGTCCGCA CCTTCTCCCA ACAGGAGATG GCCTTTAACT TGCTTGGCCT CATGTCGCCT 
ACTATTGTCG ACTTGTGCCA GAACGAGCCT GTCTTCGCTG ATCTGAACGG CGGTCTTCAG 
TTCATCACCA ACTTGAACGA GACCATGACT AGTCAGCGCA AGAACCTTAT GGAGACCAGC 
GATATTCGCA AGGCCGTCAC CAAGGAGACT GCTATTGAGA ACAAGATCGT CAATGGCGAG 
GACTCCGAGG CTCTGTACAA GAAGAAGGTC ATCGAGCCCC GTGCCAACAT CAAGTTTGAC 
TTCCCGACCT TGCCTGACTG GAAGTCCGAG GTTGCTCCTC TTAACGACAC TCTTAAGGGT 
ATGGTTGATC TGGAGAAGGT CGTTGTTGTC ACTGGTTTCG CTGAGGTTGG CCCCTGGGGT 
AACTCCAGAA CCCGCTGGGA GATGGAGGCC TATGGTGAGT TCTCACTTGA GGGCTGCATT 
GAGATGGCCT GGATCATGGG TCTCATCAAG AACCATAACG GTCCTATTAA GGGCAAGCCC 
TACTCTGGCT GGGTTGATGC CAAGACTGGC GAGCCCGTTG ATGACAAGGA CATCAAGCCC 
AAGTACGAGA AGTACATCCT TGAACACGCT GGTATCCGTC TGATCGAGCC CGAGCTATTC 
GACGGTTACG ATCCCAACAA GAAGCAGCTT CTCCATGAGG TTGTCATTGA GGAGGATCTC 
GATCCTTTCG AGGCCACCAA GGACACCGCT GAGGAGTTTA AGAGAGAGCA TGGCGATAAG 
GTCGACATCT TTGAGATTCC CGAAACTGGT CAGTACACTG TCCGTCTGAA GAAGGGTGCC 
TCGCTCTGGA TCCCCAAGGC TCTGCGCTTT GATCGTCTGG TTGCTGGCCA GATCCCCACT 
GGCTGGGACG CCAAGCGTTA CGGTATCCCC GACGACATCA TTTCTCAGGT GGATCCCGTC 
GCTCTCTTCG TCCTTGTGTC TACTGTGGAG GCTCTTCTCT CGTCTGGTAT CACCGACCCG 
TACGAGTTCT ACAAGTACGT CCATGTCTCC GAAGTGGGTA ACTGCGTTGG TTCTGGTATG 
GGTGGTGCAA CTGCACTCCG TAACATGCAC CGCGACAGAT ACCTTGATAA GCCTGTCCAG 
AACGATATTC TTCAAGAGTC CTTCATCAAC ACCATGGCCG CGTGGGTAAA CATGCTCTTG 
ATCTCGTCTT CTGGACCCAT CAAGACGCCG GTCGGTGCTT GCGCCACCGC TGTCGAGTCT 
ATCGACATTG GCTACGAGAC CATTATGGAG GGCAAGGCCC GCGTGTGCTT CGTTGGTGGC 
TTCGATGACT TTGGCGAGGA GGGCTCGTAT GAGTTCGCCA ACATGAAGGC TACCAGCAAC 
GCTGTTGATG AGTTTGCTCA TGGTCGCACG CCCAAGGAGA TGTCCAGGCC CACTACCACC 
ACGAGAAATG GCTTCATGGA GTCGCAGGGT AGCGGTGTTC AGGTCATCAT GACTGCCAAG 
CTTGCTTTGG ACATGGGCGT CCCCATCTAT GGTGTCCTTG CTTTGACCAC CACCGCTTCC 
GACAAGATTG GTCGCTCCGT CCCTGCGCCC GGCAAGGGTG TTCTTACTAC TGCTCGCGAG 
CGCCAGGGTA GCCTCCCCTC GCCCCTGCTC GATATCAAGT ACAGACGTCG CCAGATTGAG 
CGCCGGAAGA AGCAGCTTGA GAACGAGAAG GAGGAAGAGC TTGAGTATCT CGCCGATGAG 
ATCGAGGCCC TCAAGGCCGA AGGCAGGCCG CAGAAGGAGA TTGACGACTA TGCCCGCCAC 
AGAACCCAAC ACATTGAAGA GGAGGCCGAA AGACAGGTCA AGGAAGTTCT CCGCAGCTAC 
GGCAACAACT TCTGGAAGGG TGATGCTACG ATTGCTCCCC TTCGTGGTGC CCTCGCTACT 
TGGGGCCTCA CTATTGACGA TCTTGACGTC GCCTCTTTCC ACGGTACTTC CACCAAGGCC 
AACGACAAGA ATGAGTCGTC TGTCATCTGC CAGCAGCTCC GTCATCTTGG CCGCAAGAAG 
GGCAATGCCG TGCTCGGCAT CTTCCAGAAG TACCTCACTG GTCATCCCAA GGGTGCTGCT 
GGTGCCTGGA TGATGAACGG CTGCTTGCAA GTGCTCAACA GTGGCCTCGT CCCCGGCAAC 
CGCAATGCCG ACAACGTCGA CGCTGTCATG GAGGAGTTTG ACTACATTGT TTACCCTAGC 
AAGAGTATCC AGACTGATGG TGTCAAGGCC TTCTCCGTTA CATCGTTCGG TTTCGGCCAG 
AAGGGTGCTC AGGCCATTGG TGTCCATCCC AAGTATCTTT ATGCCACTCT TGATGAGGAG 
GTATTCAACG CTTACCGCAC CAAGGTGGAG GCTCGCCAGA AGAAGACCTA CCGCTTCTTC 
CACAACGGCC TTATCAACAA CACCCTGTTC GTTGCCAAGG ATAAATCTCC CTACTCTGAT 
GATCAGCTTC AATCGGTTCT CCTTAACCCC AAGGCCCGCG TCACCGAGGA CAAGAAGACC 
GGCCAGCTCA CGTATCCCGC CAACTTCATG GAGTTGGAGC CAGCACAGAA GCTGCAGAAG 
AAGAGCGAGG AATCTCTTGA GTCGACTGTT GCCCGTGCTG CAAACCGTCT CAAGAACAGC 
AACACCCGTG TTGGCTTCGA TCTTGAGGAG ATTTCCTCCA TCAACATCGA CAATGAAACC 
TTCTTGGAAC GCAACTTCAC CGAGGCTGAG CTCCAGTACT GCAAGGCTCC TAGCACTGGT 
CGGTCGCCGC AGAAGTCGTT TGCTGGTCGC TGGAGCGCGA AGGAGGCCGT CTTTAAGTCC 
CTTGGTGTCA CCGGCCAGGG TGCTGGTGCA TCCCTGAAGG ACATTGAGAT TCTCTCAGAT 
AGCAACGGGG GTCCCGTTGT GAAGTTGCAT GGTGCTGCTT TGGAGGCCGC GAATAAGGCT 
GGCGTTTCCA AGGTCAACGT GACGCTGTCC TATTCTGACA GCCATGCTGT TGCCATTGCT 
ACGGCTCACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cel-1 ( XM_011395936.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394238.1 Neurospora crassa OR74A fatty acid synthase alpha subunit reductase, variant (cel-1), mRNA.

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CloneID	ONf01153
Clone ID Related Accession (Same CDS sequence)	XM_011395935.1 , XM_011395936.1
Accession Version	XM_011395936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	fatty acid synthase alpha subunit reductase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGCGTCCCG AACTTGAGCA AGAGCTCGCC CACACCCTAC TCGTCGAGCT CCTTGCTTAC 
CAATTCGCCT CTCCCGTGAG ATGGATTGAG ACGCAAGATG TCTTCCTCGC GGAGAAGACA 
GCCGAGCGGG TTGTCGAGAT TGGTCCCGCC GACACCCTCG GTGGTATGGC CAGGAGGACG 
CTCGCCTCCA AGTACGAAGC ATTCGACGCT GCCAAGTCAA TCCAGAGACA GATTCTTTGC 
TACAACAAGG ACGCAAAGGA GATTTACTAC GATGTAGACC CTGTGGAAGA AGAGCCTGAG 
CCGGTTGCGG CTAGCTCATC ATCTGCTGCT CCCACCCCCG CTGCCGCTGC TGCTCCGGCT 
GCTGCCGCTG CTGCTCCTCC GCCCCCGAGT GCTGGCCCCG CGGCTTCAGT TCCCGATGCC 
CCAGTTCCCG CTCTTGACAT TGTCAGGTCG TTGATCGCCC AGAAGCTCAA GAAGCCCTTT 
GCAGAGATTC CTTTGAGCAA GGCTATCAAG GACTTGGTAG GAGGCAAGTC CACTCTACAA 
AACGAAATCC TTGGTGATCT AGGCAAAGAG TTCGGATCCA CACCGGAGAA GCCTGAAGAT 
ACGCCCCTCG ATGAGCTGGG TGCTGCTATG CAGGCTACCT TCGATGGTAA CCTAGGCAAG 
ACAACCCAGG GTCTTATCGC TCGTCTTATT TCGTCCAAGA TGCCCGGTGG CTTCAACATC 
ACATCAGCAC GCAAATATCT CGAGACAAGG TGGGGTCTCG GTTCTGGCCG TCAGGACGGT 
GCGCTTCTGC TCGCCATCAC TATGGAGCCT CCAGCTCGTC TTGGCTCCGA GGCCGAAGCG 
AAGGCTTATC TAGATGATAT TTCTCAAAAG TATGCCTCGC ATGCTGGCAT CAGCTTGTCC 
ACCGCGGCCG CGGCTGGCCC TGCTGCCGGA GGCGGCGGCG GCATGATGAT GGATCCTGCA 
GCCATTGAGG CCCTTACCAG CGACCAGAAG GCTATGTTCA AGCAACAATT GGAGCTTATC 
GCCAGATATC TCAAGATCGA TATCCGCGCC GGAGACAAGG CTTTCCAGGC GTCTCAGGAA 
TCTTCCAAGG TCCTGCAGTC ACAGCTGGAT CTCTGGATGG CCGAGCACGG CGATTTCTAC 
GCTGCCGGCA TTGAACCTGT CTTTAGCTCC CTCAAAGCTC GTGTGTACGA TTCTTCCTGG 
AACTGGGCTC GCCAGGATGC TCTCAGCATG TACTATGATA TCATCTTCGG CAGGCTGAAG 
GCCGTTGATC GCGAGATTGT CAGCCAGTGC ATCCGCATCA TGAACCGCTC TAATCCCACC 
CTCCTCGAGT TCATGCAATA CCACATCGAC AACTGCCCTA CGGAGCGCGG AGAGACGTAT 
CAGCTTGCCA AGGAGTTGGG CTATCAGCTC ATTGAGAACT GTAAGGAGGT TCTCAACGTT 
GACCCTGTCT ACAAGGATGT TGCCGTCCCT ACCGGCCCCC GTACCACCAT TGACGCTCGT 
GGCAACATCA AGTACGAGGA GATTCCTCGT GCAAGCTGCC GCAAGCTTGA GCATTACGTA 
CAGCAGATGG CCGAGGGTGG CAAGATCTCT GAATACAGCA ACCGTACCAA GGTTGAGAAT 
GATCTTGCTC GCATTTACAA GCTGATTAAG CAGCAGCACA AGCTCTCCAA GAACTCTCAG 
CTCGAGATCA AGTCTCTGTA TGGCGACATC ATCAGGTCTC TGAGCATGAA CGAAAGCCAG 
ATTATGCCCA AGGAGTCCAG CAAGGTCAGC AGCGTCTTGT CCAAGTCGTC GTCCAAGTCC 
AAGGGCAAGA CCGAGACAAT TCCCTTCCTC CACCTGCGCA AGAAGTCCGA GCACGGCTGG 
GATTACAGCA AGAAGCTCAC CGGCCTCTAC CTTGACTGCT TGGAGCAGGC GGCCAAGAAT 
GGTGTTACCT TCGCCAACAA GTACGTGCTC ATGACTGGTG CGGGCGCCGG TTCCATCGGT 
GCCGAGGTTC TCCAGGGTCT TATCAGCGGT GGTGCCAAGG TCATCGTCAC GACCAGCCGT 
TTCTCGCGTG AAGTTACCGA GTACTACCAG TCTATGTATG CTAGATTTGG CTCTCGTGGC 
TCTCAGCTCG TTGTGGTTCC CTTTAACCAG GGTAGCGTGC AGGACATCAA CGCCTTGGTC 
GACTACATCT ACGACACCAA GACTGGTCTC GGTTGGGATC TTGACTACAT TGTTCCCTTC 
GCCGCCATCT CTGAGCAGGG TCGCCAGATT GATGGCATTG ACTCCAAGTC CGAGTTGGCT 
CACCGCATCA TGTTGACCAA CCTCATCCGC CTCCTTGGCA GCGTCAAGGC TCAGAAGGCC 
AGCCGCGGTT TCGAAACTCG TCCCGCCCAG GTCATCCTCC CGCTCTCTCC CAACCACGGT 
ACCTTTGGTA GCGATGGTCT CTACTCCGAG TCCAAGCTCG GCCTCGAGAC TCTCTTCAAC 
AGGTGGCATG CTGAGGACTG GGCCAACTAC CTCACTATCT GTGGTGCCAT CATTGGTTGG 
ACTCGTGGTA CCGGCCTCAT GTCTGGCAAC AACCTGGTTG CTGAGGGTGT TGAGAACTTT 
GGTGTCCGCA CCTTCTCCCA ACAGGAGATG GCCTTTAACT TGCTTGGCCT CATGTCGCCT 
ACTATTGTCG ACTTGTGCCA GAACGAGCCT GTCTTCGCTG ATCTGAACGG CGGTCTTCAG 
TTCATCACCA ACTTGAACGA GACCATGACT AGTCAGCGCA AGAACCTTAT GGAGACCAGC 
GATATTCGCA AGGCCGTCAC CAAGGAGACT GCTATTGAGA ACAAGATCGT CAATGGCGAG 
GACTCCGAGG CTCTGTACAA GAAGAAGGTC ATCGAGCCCC GTGCCAACAT CAAGTTTGAC 
TTCCCGACCT TGCCTGACTG GAAGTCCGAG GTTGCTCCTC TTAACGACAC TCTTAAGGGT 
ATGGTTGATC TGGAGAAGGT CGTTGTTGTC ACTGGTTTCG CTGAGGTTGG CCCCTGGGGT 
AACTCCAGAA CCCGCTGGGA GATGGAGGCC TATGGTGAGT TCTCACTTGA GGGCTGCATT 
GAGATGGCCT GGATCATGGG TCTCATCAAG AACCATAACG GTCCTATTAA GGGCAAGCCC 
TACTCTGGCT GGGTTGATGC CAAGACTGGC GAGCCCGTTG ATGACAAGGA CATCAAGCCC 
AAGTACGAGA AGTACATCCT TGAACACGCT GGTATCCGTC TGATCGAGCC CGAGCTATTC 
GACGGTTACG ATCCCAACAA GAAGCAGCTT CTCCATGAGG TTGTCATTGA GGAGGATCTC 
GATCCTTTCG AGGCCACCAA GGACACCGCT GAGGAGTTTA AGAGAGAGCA TGGCGATAAG 
GTCGACATCT TTGAGATTCC CGAAACTGGT CAGTACACTG TCCGTCTGAA GAAGGGTGCC 
TCGCTCTGGA TCCCCAAGGC TCTGCGCTTT GATCGTCTGG TTGCTGGCCA GATCCCCACT 
GGCTGGGACG CCAAGCGTTA CGGTATCCCC GACGACATCA TTTCTCAGGT GGATCCCGTC 
GCTCTCTTCG TCCTTGTGTC TACTGTGGAG GCTCTTCTCT CGTCTGGTAT CACCGACCCG 
TACGAGTTCT ACAAGTACGT CCATGTCTCC GAAGTGGGTA ACTGCGTTGG TTCTGGTATG 
GGTGGTGCAA CTGCACTCCG TAACATGCAC CGCGACAGAT ACCTTGATAA GCCTGTCCAG 
AACGATATTC TTCAAGAGTC CTTCATCAAC ACCATGGCCG CGTGGGTAAA CATGCTCTTG 
ATCTCGTCTT CTGGACCCAT CAAGACGCCG GTCGGTGCTT GCGCCACCGC TGTCGAGTCT 
ATCGACATTG GCTACGAGAC CATTATGGAG GGCAAGGCCC GCGTGTGCTT CGTTGGTGGC 
TTCGATGACT TTGGCGAGGA GGGCTCGTAT GAGTTCGCCA ACATGAAGGC TACCAGCAAC 
GCTGTTGATG AGTTTGCTCA TGGTCGCACG CCCAAGGAGA TGTCCAGGCC CACTACCACC 
ACGAGAAATG GCTTCATGGA GTCGCAGGGT AGCGGTGTTC AGGTCATCAT GACTGCCAAG 
CTTGCTTTGG ACATGGGCGT CCCCATCTAT GGTGTCCTTG CTTTGACCAC CACCGCTTCC 
GACAAGATTG GTCGCTCCGT CCCTGCGCCC GGCAAGGGTG TTCTTACTAC TGCTCGCGAG 
CGCCAGGGTA GCCTCCCCTC GCCCCTGCTC GATATCAAGT ACAGACGTCG CCAGATTGAG 
CGCCGGAAGA AGCAGCTTGA GAACGAGAAG GAGGAAGAGC TTGAGTATCT CGCCGATGAG 
ATCGAGGCCC TCAAGGCCGA AGGCAGGCCG CAGAAGGAGA TTGACGACTA TGCCCGCCAC 
AGAACCCAAC ACATTGAAGA GGAGGCCGAA AGACAGGTCA AGGAAGTTCT CCGCAGCTAC 
GGCAACAACT TCTGGAAGGG TGATGCTACG ATTGCTCCCC TTCGTGGTGC CCTCGCTACT 
TGGGGCCTCA CTATTGACGA TCTTGACGTC GCCTCTTTCC ACGGTACTTC CACCAAGGCC 
AACGACAAGA ATGAGTCGTC TGTCATCTGC CAGCAGCTCC GTCATCTTGG CCGCAAGAAG 
GGCAATGCCG TGCTCGGCAT CTTCCAGAAG TACCTCACTG GTCATCCCAA GGGTGCTGCT 
GGTGCCTGGA TGATGAACGG CTGCTTGCAA GTGCTCAACA GTGGCCTCGT CCCCGGCAAC 
CGCAATGCCG ACAACGTCGA CGCTGTCATG GAGGAGTTTG ACTACATTGT TTACCCTAGC 
AAGAGTATCC AGACTGATGG TGTCAAGGCC TTCTCCGTTA CATCGTTCGG TTTCGGCCAG 
AAGGGTGCTC AGGCCATTGG TGTCCATCCC AAGTATCTTT ATGCCACTCT TGATGAGGAG 
GTATTCAACG CTTACCGCAC CAAGGTGGAG GCTCGCCAGA AGAAGACCTA CCGCTTCTTC 
CACAACGGCC TTATCAACAA CACCCTGTTC GTTGCCAAGG ATAAATCTCC CTACTCTGAT 
GATCAGCTTC AATCGGTTCT CCTTAACCCC AAGGCCCGCG TCACCGAGGA CAAGAAGACC 
GGCCAGCTCA CGTATCCCGC CAACTTCATG GAGTTGGAGC CAGCACAGAA GCTGCAGAAG 
AAGAGCGAGG AATCTCTTGA GTCGACTGTT GCCCGTGCTG CAAACCGTCT CAAGAACAGC 
AACACCCGTG TTGGCTTCGA TCTTGAGGAG ATTTCCTCCA TCAACATCGA CAATGAAACC 
TTCTTGGAAC GCAACTTCAC CGAGGCTGAG CTCCAGTACT GCAAGGCTCC TAGCACTGGT 
CGGTCGCCGC AGAAGTCGTT TGCTGGTCGC TGGAGCGCGA AGGAGGCCGT CTTTAAGTCC 
CTTGGTGTCA CCGGCCAGGG TGCTGGTGCA TCCCTGAAGG ACATTGAGAT TCTCTCAGAT 
AGCAACGGGG GTCCCGTTGT GAAGTTGCAT GGTGCTGCTT TGGAGGCCGC GAATAAGGCT 
GGCGTTTCCA AGGTCAACGT GACGCTGTCC TATTCTGACA GCCATGCTGT TGCCATTGCT 
ACGGCTCACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394238.1
CDS	402..5996
Translation	MRPELEQELAHTLLVELLAYQFASPVRWIETQDVFLAEKTAERVVEIGPADTLGGMARRTLASKYEAFDAAKSIQRQILCYNKDAKEIYYDVDPVEEEPEPVAASSSSAAPTPAAAAAPAAAAAAPPPPSAGPAASVPDAPVPALDIVRSLIAQKLKKPFAEIPLSKAIKDLVGGKSTLQNEILGDLGKEFGSTPEKPEDTPLDELGAAMQATFDGNLGKTTQGLIARLISSKMPGGFNITSARKYLETRWGLGSGRQDGALLLAITMEPPARLGSEAEAKAYLDDISQKYASHAGISLSTAAAAGPAAGGGGGMMMDPAAIEALTSDQKAMFKQQLELIARYLKIDIRAGDKAFQASQESSKVLQSQLDLWMAEHGDFYAAGIEPVFSSLKARVYDSSWNWARQDALSMYYDIIFGRLKAVDREIVSQCIRIMNRSNPTLLEFMQYHIDNCPTERGETYQLAKELGYQLIENCKEVLNVDPVYKDVAVPTGPRTTIDARGNIKYEEIPRASCRKLEHYVQQMAEGGKISEYSNRTKVENDLARIYKLIKQQHKLSKNSQLEIKSLYGDIIRSLSMNESQIMPKESSKVSSVLSKSSSKSKGKTETIPFLHLRKKSEHGWDYSKKLTGLYLDCLEQAAKNGVTFANKYVLMTGAGAGSIGAEVLQGLISGGAKVIVTTSRFSREVTEYYQSMYARFGSRGSQLVVVPFNQGSVQDINALVDYIYDTKTGLGWDLDYIVPFAAISEQGRQIDGIDSKSELAHRIMLTNLIRLLGSVKAQKASRGFETRPAQVILPLSPNHGTFGSDGLYSESKLGLETLFNRWHAEDWANYLTICGAIIGWTRGTGLMSGNNLVAEGVENFGVRTFSQQEMAFNLLGLMSPTIVDLCQNEPVFADLNGGLQFITNLNETMTSQRKNLMETSDIRKAVTKETAIENKIVNGEDSEALYKKKVIEPRANIKFDFPTLPDWKSEVAPLNDTLKGMVDLEKVVVVTGFAEVGPWGNSRTRWEMEAYGEFSLEGCIEMAWIMGLIKNHNGPIKGKPYSGWVDAKTGEPVDDKDIKPKYEKYILEHAGIRLIEPELFDGYDPNKKQLLHEVVIEEDLDPFEATKDTAEEFKREHGDKVDIFEIPETGQYTVRLKKGASLWIPKALRFDRLVAGQIPTGWDAKRYGIPDDIISQVDPVALFVLVSTVEALLSSGITDPYEFYKYVHVSEVGNCVGSGMGGATALRNMHRDRYLDKPVQNDILQESFINTMAAWVNMLLISSSGPIKTPVGACATAVESIDIGYETIMEGKARVCFVGGFDDFGEEGSYEFANMKATSNAVDEFAHGRTPKEMSRPTTTTRNGFMESQGSGVQVIMTAKLALDMGVPIYGVLALTTTASDKIGRSVPAPGKGVLTTARERQGSLPSPLLDIKYRRRQIERRKKQLENEKEEELEYLADEIEALKAEGRPQKEIDDYARHRTQHIEEEAERQVKEVLRSYGNNFWKGDATIAPLRGALATWGLTIDDLDVASFHGTSTKANDKNESSVICQQLRHLGRKKGNAVLGIFQKYLTGHPKGAAGAWMMNGCLQVLNSGLVPGNRNADNVDAVMEEFDYIVYPSKSIQTDGVKAFSVTSFGFGQKGAQAIGVHPKYLYATLDEEVFNAYRTKVEARQKKTYRFFHNGLINNTLFVAKDKSPYSDDQLQSVLLNPKARVTEDKKTGQLTYPANFMELEPAQKLQKKSEESLESTVARAANRLKNSNTRVGFDLEEISSINIDNETFLERNFTEAELQYCKAPSTGRSPQKSFAGRWSAKEAVFKSLGVTGQGAGASLKDIEILSDSNGGPVVKLHGAALEAANKAGVSKVNVTLSYSDSHAVAIATAHF

Target ORF information:

RefSeq Version	XM_011395936.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A fatty acid synthase alpha subunit reductase, variant (cel-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395936.1

ORF Insert Sequence:

ATGCGTCCCG AACTTGAGCA AGAGCTCGCC CACACCCTAC TCGTCGAGCT CCTTGCTTAC 
CAATTCGCCT CTCCCGTGAG ATGGATTGAG ACGCAAGATG TCTTCCTCGC GGAGAAGACA 
GCCGAGCGGG TTGTCGAGAT TGGTCCCGCC GACACCCTCG GTGGTATGGC CAGGAGGACG 
CTCGCCTCCA AGTACGAAGC ATTCGACGCT GCCAAGTCAA TCCAGAGACA GATTCTTTGC 
TACAACAAGG ACGCAAAGGA GATTTACTAC GATGTAGACC CTGTGGAAGA AGAGCCTGAG 
CCGGTTGCGG CTAGCTCATC ATCTGCTGCT CCCACCCCCG CTGCCGCTGC TGCTCCGGCT 
GCTGCCGCTG CTGCTCCTCC GCCCCCGAGT GCTGGCCCCG CGGCTTCAGT TCCCGATGCC 
CCAGTTCCCG CTCTTGACAT TGTCAGGTCG TTGATCGCCC AGAAGCTCAA GAAGCCCTTT 
GCAGAGATTC CTTTGAGCAA GGCTATCAAG GACTTGGTAG GAGGCAAGTC CACTCTACAA 
AACGAAATCC TTGGTGATCT AGGCAAAGAG TTCGGATCCA CACCGGAGAA GCCTGAAGAT 
ACGCCCCTCG ATGAGCTGGG TGCTGCTATG CAGGCTACCT TCGATGGTAA CCTAGGCAAG 
ACAACCCAGG GTCTTATCGC TCGTCTTATT TCGTCCAAGA TGCCCGGTGG CTTCAACATC 
ACATCAGCAC GCAAATATCT CGAGACAAGG TGGGGTCTCG GTTCTGGCCG TCAGGACGGT 
GCGCTTCTGC TCGCCATCAC TATGGAGCCT CCAGCTCGTC TTGGCTCCGA GGCCGAAGCG 
AAGGCTTATC TAGATGATAT TTCTCAAAAG TATGCCTCGC ATGCTGGCAT CAGCTTGTCC 
ACCGCGGCCG CGGCTGGCCC TGCTGCCGGA GGCGGCGGCG GCATGATGAT GGATCCTGCA 
GCCATTGAGG CCCTTACCAG CGACCAGAAG GCTATGTTCA AGCAACAATT GGAGCTTATC 
GCCAGATATC TCAAGATCGA TATCCGCGCC GGAGACAAGG CTTTCCAGGC GTCTCAGGAA 
TCTTCCAAGG TCCTGCAGTC ACAGCTGGAT CTCTGGATGG CCGAGCACGG CGATTTCTAC 
GCTGCCGGCA TTGAACCTGT CTTTAGCTCC CTCAAAGCTC GTGTGTACGA TTCTTCCTGG 
AACTGGGCTC GCCAGGATGC TCTCAGCATG TACTATGATA TCATCTTCGG CAGGCTGAAG 
GCCGTTGATC GCGAGATTGT CAGCCAGTGC ATCCGCATCA TGAACCGCTC TAATCCCACC 
CTCCTCGAGT TCATGCAATA CCACATCGAC AACTGCCCTA CGGAGCGCGG AGAGACGTAT 
CAGCTTGCCA AGGAGTTGGG CTATCAGCTC ATTGAGAACT GTAAGGAGGT TCTCAACGTT 
GACCCTGTCT ACAAGGATGT TGCCGTCCCT ACCGGCCCCC GTACCACCAT TGACGCTCGT 
GGCAACATCA AGTACGAGGA GATTCCTCGT GCAAGCTGCC GCAAGCTTGA GCATTACGTA 
CAGCAGATGG CCGAGGGTGG CAAGATCTCT GAATACAGCA ACCGTACCAA GGTTGAGAAT 
GATCTTGCTC GCATTTACAA GCTGATTAAG CAGCAGCACA AGCTCTCCAA GAACTCTCAG 
CTCGAGATCA AGTCTCTGTA TGGCGACATC ATCAGGTCTC TGAGCATGAA CGAAAGCCAG 
ATTATGCCCA AGGAGTCCAG CAAGGTCAGC AGCGTCTTGT CCAAGTCGTC GTCCAAGTCC 
AAGGGCAAGA CCGAGACAAT TCCCTTCCTC CACCTGCGCA AGAAGTCCGA GCACGGCTGG 
GATTACAGCA AGAAGCTCAC CGGCCTCTAC CTTGACTGCT TGGAGCAGGC GGCCAAGAAT 
GGTGTTACCT TCGCCAACAA GTACGTGCTC ATGACTGGTG CGGGCGCCGG TTCCATCGGT 
GCCGAGGTTC TCCAGGGTCT TATCAGCGGT GGTGCCAAGG TCATCGTCAC GACCAGCCGT 
TTCTCGCGTG AAGTTACCGA GTACTACCAG TCTATGTATG CTAGATTTGG CTCTCGTGGC 
TCTCAGCTCG TTGTGGTTCC CTTTAACCAG GGTAGCGTGC AGGACATCAA CGCCTTGGTC 
GACTACATCT ACGACACCAA GACTGGTCTC GGTTGGGATC TTGACTACAT TGTTCCCTTC 
GCCGCCATCT CTGAGCAGGG TCGCCAGATT GATGGCATTG ACTCCAAGTC CGAGTTGGCT 
CACCGCATCA TGTTGACCAA CCTCATCCGC CTCCTTGGCA GCGTCAAGGC TCAGAAGGCC 
AGCCGCGGTT TCGAAACTCG TCCCGCCCAG GTCATCCTCC CGCTCTCTCC CAACCACGGT 
ACCTTTGGTA GCGATGGTCT CTACTCCGAG TCCAAGCTCG GCCTCGAGAC TCTCTTCAAC 
AGGTGGCATG CTGAGGACTG GGCCAACTAC CTCACTATCT GTGGTGCCAT CATTGGTTGG 
ACTCGTGGTA CCGGCCTCAT GTCTGGCAAC AACCTGGTTG CTGAGGGTGT TGAGAACTTT 
GGTGTCCGCA CCTTCTCCCA ACAGGAGATG GCCTTTAACT TGCTTGGCCT CATGTCGCCT 
ACTATTGTCG ACTTGTGCCA GAACGAGCCT GTCTTCGCTG ATCTGAACGG CGGTCTTCAG 
TTCATCACCA ACTTGAACGA GACCATGACT AGTCAGCGCA AGAACCTTAT GGAGACCAGC 
GATATTCGCA AGGCCGTCAC CAAGGAGACT GCTATTGAGA ACAAGATCGT CAATGGCGAG 
GACTCCGAGG CTCTGTACAA GAAGAAGGTC ATCGAGCCCC GTGCCAACAT CAAGTTTGAC 
TTCCCGACCT TGCCTGACTG GAAGTCCGAG GTTGCTCCTC TTAACGACAC TCTTAAGGGT 
ATGGTTGATC TGGAGAAGGT CGTTGTTGTC ACTGGTTTCG CTGAGGTTGG CCCCTGGGGT 
AACTCCAGAA CCCGCTGGGA GATGGAGGCC TATGGTGAGT TCTCACTTGA GGGCTGCATT 
GAGATGGCCT GGATCATGGG TCTCATCAAG AACCATAACG GTCCTATTAA GGGCAAGCCC 
TACTCTGGCT GGGTTGATGC CAAGACTGGC GAGCCCGTTG ATGACAAGGA CATCAAGCCC 
AAGTACGAGA AGTACATCCT TGAACACGCT GGTATCCGTC TGATCGAGCC CGAGCTATTC 
GACGGTTACG ATCCCAACAA GAAGCAGCTT CTCCATGAGG TTGTCATTGA GGAGGATCTC 
GATCCTTTCG AGGCCACCAA GGACACCGCT GAGGAGTTTA AGAGAGAGCA TGGCGATAAG 
GTCGACATCT TTGAGATTCC CGAAACTGGT CAGTACACTG TCCGTCTGAA GAAGGGTGCC 
TCGCTCTGGA TCCCCAAGGC TCTGCGCTTT GATCGTCTGG TTGCTGGCCA GATCCCCACT 
GGCTGGGACG CCAAGCGTTA CGGTATCCCC GACGACATCA TTTCTCAGGT GGATCCCGTC 
GCTCTCTTCG TCCTTGTGTC TACTGTGGAG GCTCTTCTCT CGTCTGGTAT CACCGACCCG 
TACGAGTTCT ACAAGTACGT CCATGTCTCC GAAGTGGGTA ACTGCGTTGG TTCTGGTATG 
GGTGGTGCAA CTGCACTCCG TAACATGCAC CGCGACAGAT ACCTTGATAA GCCTGTCCAG 
AACGATATTC TTCAAGAGTC CTTCATCAAC ACCATGGCCG CGTGGGTAAA CATGCTCTTG 
ATCTCGTCTT CTGGACCCAT CAAGACGCCG GTCGGTGCTT GCGCCACCGC TGTCGAGTCT 
ATCGACATTG GCTACGAGAC CATTATGGAG GGCAAGGCCC GCGTGTGCTT CGTTGGTGGC 
TTCGATGACT TTGGCGAGGA GGGCTCGTAT GAGTTCGCCA ACATGAAGGC TACCAGCAAC 
GCTGTTGATG AGTTTGCTCA TGGTCGCACG CCCAAGGAGA TGTCCAGGCC CACTACCACC 
ACGAGAAATG GCTTCATGGA GTCGCAGGGT AGCGGTGTTC AGGTCATCAT GACTGCCAAG 
CTTGCTTTGG ACATGGGCGT CCCCATCTAT GGTGTCCTTG CTTTGACCAC CACCGCTTCC 
GACAAGATTG GTCGCTCCGT CCCTGCGCCC GGCAAGGGTG TTCTTACTAC TGCTCGCGAG 
CGCCAGGGTA GCCTCCCCTC GCCCCTGCTC GATATCAAGT ACAGACGTCG CCAGATTGAG 
CGCCGGAAGA AGCAGCTTGA GAACGAGAAG GAGGAAGAGC TTGAGTATCT CGCCGATGAG 
ATCGAGGCCC TCAAGGCCGA AGGCAGGCCG CAGAAGGAGA TTGACGACTA TGCCCGCCAC 
AGAACCCAAC ACATTGAAGA GGAGGCCGAA AGACAGGTCA AGGAAGTTCT CCGCAGCTAC 
GGCAACAACT TCTGGAAGGG TGATGCTACG ATTGCTCCCC TTCGTGGTGC CCTCGCTACT 
TGGGGCCTCA CTATTGACGA TCTTGACGTC GCCTCTTTCC ACGGTACTTC CACCAAGGCC 
AACGACAAGA ATGAGTCGTC TGTCATCTGC CAGCAGCTCC GTCATCTTGG CCGCAAGAAG 
GGCAATGCCG TGCTCGGCAT CTTCCAGAAG TACCTCACTG GTCATCCCAA GGGTGCTGCT 
GGTGCCTGGA TGATGAACGG CTGCTTGCAA GTGCTCAACA GTGGCCTCGT CCCCGGCAAC 
CGCAATGCCG ACAACGTCGA CGCTGTCATG GAGGAGTTTG ACTACATTGT TTACCCTAGC 
AAGAGTATCC AGACTGATGG TGTCAAGGCC TTCTCCGTTA CATCGTTCGG TTTCGGCCAG 
AAGGGTGCTC AGGCCATTGG TGTCCATCCC AAGTATCTTT ATGCCACTCT TGATGAGGAG 
GTATTCAACG CTTACCGCAC CAAGGTGGAG GCTCGCCAGA AGAAGACCTA CCGCTTCTTC 
CACAACGGCC TTATCAACAA CACCCTGTTC GTTGCCAAGG ATAAATCTCC CTACTCTGAT 
GATCAGCTTC AATCGGTTCT CCTTAACCCC AAGGCCCGCG TCACCGAGGA CAAGAAGACC 
GGCCAGCTCA CGTATCCCGC CAACTTCATG GAGTTGGAGC CAGCACAGAA GCTGCAGAAG 
AAGAGCGAGG AATCTCTTGA GTCGACTGTT GCCCGTGCTG CAAACCGTCT CAAGAACAGC 
AACACCCGTG TTGGCTTCGA TCTTGAGGAG ATTTCCTCCA TCAACATCGA CAATGAAACC 
TTCTTGGAAC GCAACTTCAC CGAGGCTGAG CTCCAGTACT GCAAGGCTCC TAGCACTGGT 
CGGTCGCCGC AGAAGTCGTT TGCTGGTCGC TGGAGCGCGA AGGAGGCCGT CTTTAAGTCC 
CTTGGTGTCA CCGGCCAGGG TGCTGGTGCA TCCCTGAAGG ACATTGAGAT TCTCTCAGAT 
AGCAACGGGG GTCCCGTTGT GAAGTTGCAT GGTGCTGCTT TGGAGGCCGC GAATAAGGCT 
GGCGTTTCCA AGGTCAACGT GACGCTGTCC TATTCTGACA GCCATGCTGT TGCCATTGCT 
ACGGCTCACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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cel-1 cDNA ORF clone, Neurospora crassa OR74A

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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