spo11 cDNA ORF clone, Neurospora crassa OR74A

The following spo11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spo11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf11647 XM_955934.3
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Neurospora crassa OR74A meiosis-specific topoisomerase Spo11 (spo11), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf11647
    Clone ID Related Accession (Same CDS sequence) XM_955934.3
    Accession Version XM_955934.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product meiosis-specific topoisomerase Spo11
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505). On Feb 23, 2015 this sequence version replaced XM_955934.2. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTATTTTT GTCCGATACG CAACCAGTAC CTGCATCCAC CTTCTCAACC CACGGCTACC 
    AACTCCCATG AACGGCCCTC TCGTCCTCTA GAGCAGCTTT CTCCGGTAAG AGCTAGCAGT
    GCTAGAGTAC CTCACAACAT CGTGATCGTA CCACAGAGCC ACTCTAGCGA TAATGCCCTC
    ACAAAGATCG AGGCCTTGTT CGAGACCATT GTGGATGCTA TCAGTGTCGG AAGTCCCATT
    ACCATACCAT ATCGGCGGTC GACTAACGCG CAAGTATTCG ACCCGACTGC CTCTGCCTCA
    AATCCAAGGG ATGGGCGGCA CATGGATTCC GTACGCTTTC CGGGACGCAA CCCGCAAGAG
    CTCCGAAGAT TTGAAGCCTT GTTCCGCATC ATCGAGATTT CTCACGAAGC ACTCCTGTCT
    GGAACTCTAG TCACAAAGAG GAACATTTAC TACCAAAACA TGGAATTATT CAGGTCACAG
    AGCGTGGTGG ATGACATGGT GGACAATCTT GCATTCACAC TTGGAGTGGG ACGTAATGAT
    TTGAATATTG TCGCCACCGC AAAGGGCCTC GTTGCTGGCC AGGTAGAACT CATCATGCGT
    GGCGGTTCCA AGATCGATTG TGCCGAGTCT TCCGATAGTG GTGTTTTGTT GCCATCAATT
    AGCGCAGTTG GGAAGATCGA CTTTCAGTCC GTCAAATGGC TGCTGGTCAT TGAAAAGGAG
    GCCACCTTTC GAACACTCTC CGCTTCGCGC TACTATACAG TCTCTCGAGC AGGAAGTGGT
    ATCTTGGTGA CTGGAAAGGG ATATCCCGAC CTTGCTACAC GAAAGTTCCT CGCCGCCATC
    CACTCGGTTC GACCGCGTTT ACTCATGTTT GCCCTGGTCG ACTTCGATCC CCATGGGATC
    TCTGTTCTAC GGACTTACCA ATATGGCAGC CAACGTCTGG ACCACGAGGA GCGCACCAAG
    GTGTTTGGAT TAAGATGGCT CGGAATTCGC AGCAGCGATG TCCTCTTAAA CACAGTGATG
    ACGCAAGATT GCTCCGCCAA CAGTCAGGGA AGTCAATCGA GCCAAGACCT CTCAAGTCAG
    GAATCAATAG CTTATTCTGT GGATGCATTC GAAGATGAAC GTCCGGCGAA GAGGCCAAGA
    AGTCGAAGAA CGAAAAGCCA TCCAAGCGAC TACACAGCGT CACTCAGCGA AAGGGACAGG
    GAGAAAGCCG TCAGCGTTTT GCGAGACATA TGCGGTACGG CGAGACTAGA CGCTACAGAG
    CGGGAGCACA AGCTTGAATT GCAGAGAATG TTGATGCTCA ACATCAAGGC GGAGATACAG
    GCGGTCGACG ATTTTGGCGA TATTACGAAC TGGCTGGATG AACGACTGTT GAAACACAGC
    ATGGATGTGA ACTCGCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_961027.3
    CDS1..1401
    Translation

    Target ORF information:

    RefSeq Version XM_955934.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A meiosis-specific topoisomerase Spo11 (spo11), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_955934.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTATTTTT GTCCGATACG CAACCAGTAC CTGCATCCAC CTTCTCAACC CACGGCTACC 
    AACTCCCATG AACGGCCCTC TCGTCCTCTA GAGCAGCTTT CTCCGGTAAG AGCTAGCAGT
    GCTAGAGTAC CTCACAACAT CGTGATCGTA CCACAGAGCC ACTCTAGCGA TAATGCCCTC
    ACAAAGATCG AGGCCTTGTT CGAGACCATT GTGGATGCTA TCAGTGTCGG AAGTCCCATT
    ACCATACCAT ATCGGCGGTC GACTAACGCG CAAGTATTCG ACCCGACTGC CTCTGCCTCA
    AATCCAAGGG ATGGGCGGCA CATGGATTCC GTACGCTTTC CGGGACGCAA CCCGCAAGAG
    CTCCGAAGAT TTGAAGCCTT GTTCCGCATC ATCGAGATTT CTCACGAAGC ACTCCTGTCT
    GGAACTCTAG TCACAAAGAG GAACATTTAC TACCAAAACA TGGAATTATT CAGGTCACAG
    AGCGTGGTGG ATGACATGGT GGACAATCTT GCATTCACAC TTGGAGTGGG ACGTAATGAT
    TTGAATATTG TCGCCACCGC AAAGGGCCTC GTTGCTGGCC AGGTAGAACT CATCATGCGT
    GGCGGTTCCA AGATCGATTG TGCCGAGTCT TCCGATAGTG GTGTTTTGTT GCCATCAATT
    AGCGCAGTTG GGAAGATCGA CTTTCAGTCC GTCAAATGGC TGCTGGTCAT TGAAAAGGAG
    GCCACCTTTC GAACACTCTC CGCTTCGCGC TACTATACAG TCTCTCGAGC AGGAAGTGGT
    ATCTTGGTGA CTGGAAAGGG ATATCCCGAC CTTGCTACAC GAAAGTTCCT CGCCGCCATC
    CACTCGGTTC GACCGCGTTT ACTCATGTTT GCCCTGGTCG ACTTCGATCC CCATGGGATC
    TCTGTTCTAC GGACTTACCA ATATGGCAGC CAACGTCTGG ACCACGAGGA GCGCACCAAG
    GTGTTTGGAT TAAGATGGCT CGGAATTCGC AGCAGCGATG TCCTCTTAAA CACAGTGATG
    ACGCAAGATT GCTCCGCCAA CAGTCAGGGA AGTCAATCGA GCCAAGACCT CTCAAGTCAG
    GAATCAATAG CTTATTCTGT GGATGCATTC GAAGATGAAC GTCCGGCGAA GAGGCCAAGA
    AGTCGAAGAA CGAAAAGCCA TCCAAGCGAC TACACAGCGT CACTCAGCGA AAGGGACAGG
    GAGAAAGCCG TCAGCGTTTT GCGAGACATA TGCGGTACGG CGAGACTAGA CGCTACAGAG
    CGGGAGCACA AGCTTGAATT GCAGAGAATG TTGATGCTCA ACATCAAGGC GGAGATACAG
    GCGGTCGACG ATTTTGGCGA TATTACGAAC TGGCTGGATG AACGACTGTT GAAACACAGC
    ATGGATGTGA ACTCGCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B