crf8-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	crf8-1
Entrez Gene ID	3874748
Full Name	chromatin remodelling factor 8-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395637.1	XP_011393939.1	TBP associated factor, variant
XM_011395636.1	XP_011393938.1	TBP associated factor

Pathways from BioSystems

Homologies

Homo sapiens (human)	BTAF1	NP_003963.1
Danio rerio (zebrafish)	btaf1	XP_005169589.1
Saccharomyces cerevisiae (baker's yeast)	MOT1	NP_015243.1
Gallus gallus (chicken)	BTAF1	XP_421689.3
Eremothecium gossypii	AGOS_AEL256C	NP_984605.2
Magnaporthe oryzae (rice blast fungus)	MGG_04989	XP_003712489.1
Pan troglodytes (chimpanzee)	BTAF1	XP_003312799.1
Canis lupus familiaris (dog)	BTAF1	XP_003640032.1
Bos taurus (cattle)	BTAF1	XP_003587915.2
Arabidopsis thaliana (thale cress)	RGD3	NP_190996.3
Rattus norvegicus (Norway rat)	Btaf1	NP_001178846.1
Drosophila melanogaster (fruit fly)	Hel89B	NP_732097.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001820	XP_321235.5
Schizosaccharomyces pombe (fission yeast)	mot1	NP_596080.2
	BTAF1	XP_002805795.1
Mus musculus (house mouse)	Btaf1	NP_001074175.1
Kluyveromyces lactis	KLLA0E23717g	XP_455023.1
Neurospora crassa	NCU07556	XP_958601.1
Xenopus tropicalis (tropical clawed frog)	btaf1	XP_002939139.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crf8-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crf8-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf00567	XM_011395636.1 Latest version!	Neurospora crassa OR74A TBP associated factor (crf8-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf00567	XM_011395637.1 Latest version!	Neurospora crassa OR74A TBP associated factor, variant (crf8-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crf8-1 ( XM_011395637.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393939.1 Neurospora crassa OR74A TBP associated factor, variant (crf8-1), mRNA.

Price & Availability↑

CloneID	ONf00567
Clone ID Related Accession (Same CDS sequence)	XM_011395637.1 , XM_011395636.1
Accession Version	XM_011395637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	TBP associated factor, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503).

ATGGCGTCTC GGCTAGATCG CCTTGTCACG CTTATCGAGA CGGGCAGTAC TCGCCTGATT 
CGGGATGCTG CCGTAGATCA GCTCGCCACA TGGCAAAAGG AACACCCCGA TGAGCTTTTC 
AATCTACTCT CTCGGGTTGT CCCCTACTTG CGCCACAAAG ACTGGGAAAC CAGATCAACT 
GCTGCCAAGG CTATAGGCAA AATCATCGAA AATGCACCGC AGTATGATCC CAATGCGGAC 
GAGGAACCCG ACGCGGCGAT GAAAGATGAA GATAGCAATA GCTTCATCAA GAAAGAAGAG 
GGCCCCCCAA AGCTCTCTCT TAGTGAGGAG GAGCAGTTCA GTCTTATGAC TCTGGACGTT 
ATCAACATTC TCAAGTACGG AAGGTCCTTG CTCAGAATTG GCGACAAAAG CGATTGGGGC 
CTTGCAGCTC TCGATCCACA GAGTCGGCTG AACCATCAGA AAAGAACGCT CAACGGCCGT 
CTGGGTCTCC TTGGACGTGA GTTTAAGGAT GAAGAAATAC CCGTCATTAA CGACGCCATC 
CTCAGCCCTG CGACCCCTCT CGATCCAGCT GCAGCTAATG GCTTCGGCCG TCATGACAGT 
GTCGGTTCCG CCCAAACTCA GAACGGTGAG ACGGCCGGGC TTAGTGCACG ACAGCTCAAT 
GTTCTCAAGC GAAAGCGGAA GAAGGAGGCT CAGAAGGCTG CTCAGGGCAA GAGCGGCTTC 
GGTGACCTCT CCTTGCGCCG AAACACAACC GCCGGCTCGG AGGGCTTTGA TGACACGCCG 
ATGGTGGATG CCGACTCCAA GGGCGGCAAG ATGAATGACT ATTTCAGCCT CGATAGGCCC 
GCTGATATTG AAGAGGACAC TAAGGTTGTC AGCGAATACA AGGGGCCCAT TATCCCCATC 
AAGTCCGAGA TTGAGGCCGA CGACAACTTG GAAGGGGTTG AATGGCCATA CGAACGTCTC 
TGCGATTTCC TCAAGGTCGA CCTGTTTGAT CCTCAGTGGG AAACTCGTCA TGGCGCTGCC 
ATGGGCTTGC GCGAGGTCAT CAGGGTCCAT GGCGCTGGCG CGGGAAGATT GATGGCCAAG 
TCTCGATCAG AGAACAACGA GCTCAATCGG AAGTGGCTGG ATGACCTCGC TTGTCGCCTA 
TGCTGCGTTT TGATGCTTGA CAGGTTCACA GATTATAGTT CTGATACCTC TGTGGCACCT 
ATTCGTGAGA CCGTCGGCCA GACACTTGGA TCCGTCCTTC GTCATTGCCC TCCGGCAACG 
GTCCATGCTA TTTACGAACT GCTGTTCCGC ATGGTGATGC AGAAAGATCT TCAGCTCAAC 
CACCATGAGT GGGCCATTTG CCACGGCGGC ATGATTGGCC TTCGTTATGT TGTTGCTGTG 
AGGAAGGACC TGCTCCTGCA GGATACCGCC ATGATTGATG GCGTTATTCT TGCTGTCATG 
GAAGGATTGG CCAACCAGGA CGACGATGTT CGCTCGGTCA GCGCGGCGAC CCTGATACCC 
ATGGCCAAGG AGTTTGTTAA GATGCGCTCG GATGCCCTTC CTGATCTCAT CAACATCGTT 
TGGGAAAGTC TCTCTAACCT TGGCGACGAT CTCAGTGCGT CCACAGGCAA GATCATGGAC 
CTCTTGGCGA CTCTTTGCAG TTTCCCAGAA GTCTTGGAAA CCATGAAGCA CTCGGCGTCG 
CACGATGAGG AGCGGTCGTT TACACTCCTT GTCCCGAGGC TTTACCCCTT CCTTCGTCAT 
ACCATTACCT CTGTCAGGCT TGCTGTCCTC AAGGCTTTGA TGACCTTCGC CAAGCTCGGA 
GGCGACACAA CGCAAGGATG GCTTGGCGGT AGGATTCTGC GTTTAATCTT CCAAAACATC 
CTTGTCGAGA GGGATCAGGA AACACTGAAT GCATCTGTCG ATCTTTGGGC AGCCCTCGTC 
CGTACACTTG CTAACAACAA CCCTGCGATA CTCGCTGATG AGTTTGGACC ACATGTGGAC 
ACGCTCATGC AGCTGACGCT CCATCCTATC GGTGTGTCTA GAAACCCCAT TCCCATGAAT 
GCGAGCTTGT TCCAGAAGCC TTCTGGCGGA ACCTATTCCA TGCCAGGGCT TGTCGCGGGT 
GGTAACAGGA GGTCATCGCC ACCCGAAGGC GCGGACAGGA CTGTCAAACG GAGGAGAAAA 
TCGACCAAGG TCGACGAGCC TCCTCCAACT TCCCATACTC ACGATGTCGA TGGCCACATG 
ATGCAGGGAG ATGTCGACCT CGTTGGCATG GATGTTCTGA TCCGCTCCCG CGTCTCGGCA 
GCCAAGGCCA TGGGCTTAGT CATGGCCCTG GTCCCACCAT CAACCGTTGA AGCCTACGAT 
GCCAGTATCA TCCCGGCCCT CTCATCCGCA TACTCTTCAA CTCAGCTTAC TGCCAGCATT 
GTCATTGACG AGTACGCCAA GAACAGCGCC TCCAGTCAGG CATCAACTCG TTTCGTCCAA 
CCCTTGCTCG CAATTATTGA GCAAGAACGG CCATCCCACT ACCGAGACTT GGTTACCTAT 
GTGCAGCGAG TACGGTCACA ATCGCAACAG CTGCTTAACC TTTGCCGAGA TCATGGCAAG 
GTTTCGCAGA GCAAGTTGCC CACTCTTGCT GTCGTGGTAC AGGGCGAGCC CGAAGCTGGT 
CCCGGCGCTT TCTCTATTGC GAACGCTGAG AAGGTTGTCA ACGAAGATTT TGAGAGGCTC 
AAAAAGATCA TGGCTCCTGG CCAGCGGCTG ATTGCTGTCA AGGACCTCAA TGATGCCCGC 
GATCTCGTCG TTACTGCTAT TGAGGAGGCC AAGGCGGCAA AGGAAGCTCG CGATACTCGG 
ATCCGTGCTG CAGCAGCATG CGCCTTGGTA TCCATGGAGG TGCTACCCAA GAAACCCAGC 
CCACTCATCA AGGCTATCAT GGATAGCGTC AAGGGTGAGG AGAACAATCT GCTTCAAGGT 
CGCTCGGCTA CCAGCGTCGC GCGTCTGGTA CAGCTCTTCA CCGAGTCTGG CCGCCGCGGC 
CCTGGTGACA AGATTGTTGC CAACTTGGTC AAGTATACTT GCGTGGAAAC TGCGGAAACC 
CCCGAGTTCC CTCAGCACGC TCACAAGACA AACGTTATTC TTTCCATGTC CAAAGAGGAG 
GACAGAGCCG AGCACGAGGA TCCAATTAAA TACGCGCTCG AAGCAAAGAA AGCTCACATT 
ACTCGGCGCG GTGCCAAGCA GGCTCTGGAG GTGCTGTCGC AGACATACGG ACCTGAGCTT 
TTGAGCCGGG TGCCTAGTCT CCGCACCTTC ATGGAGGAGC CTTTGGTCAG AGCCTTCTCG 
GGTGAACTCC CAGCAGAGGC CAGAGATCCT TCGAGCACGT TTGGTCAAGA GATTGTGGAT 
GCCCTGTCCA TCATTCGCAC CATGACCCCA ACACTCCACA CCGCACTTCA CTCATTCGTC 
ATGCAACAAG TGCCCTTTGT CATCAAGGCT CTCCAGTCCC AGCTTTCGGT CTTCCGATAC 
ATGGCTGCCA AGTGTATGGC CACCATCTGC TCCGTCATCA CTGTCGATGG TATGACCGCA 
CTTGTGGAAA AGGTTCTGCC CTCAATCAAC AACCCTGTCG ATCTCAACTT CCGCCAGGGC 
GCCATTGAGG TTATCTATCA TTTGATTGCA GTCATGGGAG ACGCCATCCT GCCCTATGTG 
ATTTTCCTGA TCGTGCCTGT CCTTGGTCGT ATGAGCGATT CCGATGACAA CATCCGTCTT 
ATTGCGACCA CATCATTCGC TACTCTTGTC AAGCTCGTAC CTCTTGAGGC TGGAATTCCT 
GATCCTCCCG GTCTTTCAGA GGAGCTCTTG CGTGGCCGAG ACCGTGAACG AACGTTCATT 
CAGCAGCTGC TTGACCCGAA GAAGGTCGAG CCGTTCCAGA TTCCTGTAGC CATCAAGGCA 
GAACTGCGTT CTTACCAGCA GGAAGGTGTC AACTGGCTCC ATTTCCTCAA CAAATATCAC 
CTCCATGGTA TTCTCTGCGA CGACATGGGC CTCGGCAAGA CGCTTCAGAC AATTTGCATT 
GTCGCCAGTG ACCATCATCA GCGTGCTGAA GAGTTTGCCA AGACCGGTGC GCCAGATGTC 
AGAAGGTTGC CGTCTTTGAT CGTCTGCCCG CCCACCCTTT CAGGACATTG GCAGCAGGAG 
ATCAAGAACT ACGCTCCATT CCTCAGCGTT ACGACCTACG TTGGTCCACC TGCTGAGCGA 
AAGGCTATGA AAGACACTCT GGACAAGACC GACATTGTCA TCACCTCCTA TGATGTGTGC 
CGTAATGACA TCGATGTCAT CAAGAAATAC AGTTGGAACT ACGTGGTGCT TGATGAGGGA 
CACTTGATCA AGAACCCCAA AGCAAAGATC ACCATCGCCG TCAAGCAACT GGCCAGCAAC 
CACCGTCTTA TCCTCACCGG TACACCGATC CAGAACAATG TGCTAGAATT ATGGTCTTTG 
TTTGACTTCC TCATGCCCGG CTTCTTGGGT GCAGAAAAGG TGTTCCTTGA CAGATTCGCG 
AAGCCCATTG CCAACAGTCG ATATAGCAAG GCGTCCTCGA AGGAACAAGA AGCAGGCGCT 
CTGGCTATTG AGGCGCTGCA TAAGCAGGTC CTGCCCTTCC TTTTGCGACG TCTCAAGGAG 
GAAGTTCTTA ACGACTTGCC ACCCAAGATT TTGCAGAACT ACTATTGCGA CCTCAGCGAC 
CTTCAACGCA AACTTTTTGA GGACTTCACC AAGAGGGAGG CGAAGAAGAT CACCGAGGAG 
GCGGGCCGTG ATGACAAGGA AGCCAAGGCC CATATTTTCC AAGCTCTGCA GTACATGCGC 
AAGCTTTGCA ACTCTCCTGC GCTCGTCATG AAGCCCGGTC ACAAGCAGTA CGATGATACA 
CAGAAATTCC TGGCCAAACG AAACACGAGC CTCGAGGATC CGGTCCATGC CCCCAAACTC 
ACGGCACTCC GAGACTTGCT TGTCGACTGC GGTATCGGTG TTGAAGGTCA AGAATCGTCC 
GATCCGCTCT ACACGCCCAT TAAGCCTCAT CGTGCACTCA TCTTCTGCCA GATGAAGGAA 
ATGTTGGATA TGGTTCAGAA TACCGTCCTC AAGACTATGC TTCCCTCGGT ATCTCACCTT 
CGTCTTGACG GATCCGTCGA CCCTACCCGC CGTCAGGACA TTGTCAACAA GTTCAACAGT 
GACCCATCAT ACGATGTGTT GCTGCTCACC ACGAGCGTCG GTGGTCTTGG TCTCAACCTG 
ACTGGTGCAG ATACTGTTAT CTTTGTTGAG CACGATTGGA ACCCGCAGAA GGATCTGCAG 
GCCATGGACC GCGCCCATCG TATCGGTCAG AAGAAGGTGG TCAACGTTTA CCGCTTGATC 
ACCCGCGGAA CACTTGAGGA GAAGATTCTC AGTCTTCAAC GTTTCAAGAT CGACGTCGCC 
AGTACGGTAG TGAACCAGCA GAATGCAGGA CTTGCGACCA TGGATACGGA TCAGATTCTT 
GATCTGTTCA ACCTTGGTGA TGCCGGACCA AACCTCATAA CGGAGGGCAA GAACAAGGAC 
AACCTCGAGG GCAGGGAGGA GGACATGGTG GATATCGAGA CGGGCGATGT CAGGCAACCT 
GGCAAGAAAG CGGCTTGGCT CGAAGGCCTC GGGGAGCTGT GGGATAATTC ACAGTACGAG 
GAAAGCTTCG ATCTCGACGG CTTCCTGAAG ACTATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393939.1
CDS	145..5826
Translation	MASRLDRLVTLIETGSTRLIRDAAVDQLATWQKEHPDELFNLLSRVVPYLRHKDWETRSTAAKAIGKIIENAPQYDPNADEEPDAAMKDEDSNSFIKKEEGPPKLSLSEEEQFSLMTLDVINILKYGRSLLRIGDKSDWGLAALDPQSRLNHQKRTLNGRLGLLGREFKDEEIPVINDAILSPATPLDPAAANGFGRHDSVGSAQTQNGETAGLSARQLNVLKRKRKKEAQKAAQGKSGFGDLSLRRNTTAGSEGFDDTPMVDADSKGGKMNDYFSLDRPADIEEDTKVVSEYKGPIIPIKSEIEADDNLEGVEWPYERLCDFLKVDLFDPQWETRHGAAMGLREVIRVHGAGAGRLMAKSRSENNELNRKWLDDLACRLCCVLMLDRFTDYSSDTSVAPIRETVGQTLGSVLRHCPPATVHAIYELLFRMVMQKDLQLNHHEWAICHGGMIGLRYVVAVRKDLLLQDTAMIDGVILAVMEGLANQDDDVRSVSAATLIPMAKEFVKMRSDALPDLINIVWESLSNLGDDLSASTGKIMDLLATLCSFPEVLETMKHSASHDEERSFTLLVPRLYPFLRHTITSVRLAVLKALMTFAKLGGDTTQGWLGGRILRLIFQNILVERDQETLNASVDLWAALVRTLANNNPAILADEFGPHVDTLMQLTLHPIGVSRNPIPMNASLFQKPSGGTYSMPGLVAGGNRRSSPPEGADRTVKRRRKSTKVDEPPPTSHTHDVDGHMMQGDVDLVGMDVLIRSRVSAAKAMGLVMALVPPSTVEAYDASIIPALSSAYSSTQLTASIVIDEYAKNSASSQASTRFVQPLLAIIEQERPSHYRDLVTYVQRVRSQSQQLLNLCRDHGKVSQSKLPTLAVVVQGEPEAGPGAFSIANAEKVVNEDFERLKKIMAPGQRLIAVKDLNDARDLVVTAIEEAKAAKEARDTRIRAAAACALVSMEVLPKKPSPLIKAIMDSVKGEENNLLQGRSATSVARLVQLFTESGRRGPGDKIVANLVKYTCVETAETPEFPQHAHKTNVILSMSKEEDRAEHEDPIKYALEAKKAHITRRGAKQALEVLSQTYGPELLSRVPSLRTFMEEPLVRAFSGELPAEARDPSSTFGQEIVDALSIIRTMTPTLHTALHSFVMQQVPFVIKALQSQLSVFRYMAAKCMATICSVITVDGMTALVEKVLPSINNPVDLNFRQGAIEVIYHLIAVMGDAILPYVIFLIVPVLGRMSDSDDNIRLIATTSFATLVKLVPLEAGIPDPPGLSEELLRGRDRERTFIQQLLDPKKVEPFQIPVAIKAELRSYQQEGVNWLHFLNKYHLHGILCDDMGLGKTLQTICIVASDHHQRAEEFAKTGAPDVRRLPSLIVCPPTLSGHWQQEIKNYAPFLSVTTYVGPPAERKAMKDTLDKTDIVITSYDVCRNDIDVIKKYSWNYVVLDEGHLIKNPKAKITIAVKQLASNHRLILTGTPIQNNVLELWSLFDFLMPGFLGAEKVFLDRFAKPIANSRYSKASSKEQEAGALAIEALHKQVLPFLLRRLKEEVLNDLPPKILQNYYCDLSDLQRKLFEDFTKREAKKITEEAGRDDKEAKAHIFQALQYMRKLCNSPALVMKPGHKQYDDTQKFLAKRNTSLEDPVHAPKLTALRDLLVDCGIGVEGQESSDPLYTPIKPHRALIFCQMKEMLDMVQNTVLKTMLPSVSHLRLDGSVDPTRRQDIVNKFNSDPSYDVLLLTTSVGGLGLNLTGADTVIFVEHDWNPQKDLQAMDRAHRIGQKKVVNVYRLITRGTLEEKILSLQRFKIDVASTVVNQQNAGLATMDTDQILDLFNLGDAGPNLITEGKNKDNLEGREEDMVDIETGDVRQPGKKAAWLEGLGELWDNSQYEESFDLDGFLKTMQ

Target ORF information:

RefSeq Version	XM_011395637.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A TBP associated factor, variant (crf8-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395637.1

ORF Insert Sequence:

ATGGCGTCTC GGCTAGATCG CCTTGTCACG CTTATCGAGA CGGGCAGTAC TCGCCTGATT 
CGGGATGCTG CCGTAGATCA GCTCGCCACA TGGCAAAAGG AACACCCCGA TGAGCTTTTC 
AATCTACTCT CTCGGGTTGT CCCCTACTTG CGCCACAAAG ACTGGGAAAC CAGATCAACT 
GCTGCCAAGG CTATAGGCAA AATCATCGAA AATGCACCGC AGTATGATCC CAATGCGGAC 
GAGGAACCCG ACGCGGCGAT GAAAGATGAA GATAGCAATA GCTTCATCAA GAAAGAAGAG 
GGCCCCCCAA AGCTCTCTCT TAGTGAGGAG GAGCAGTTCA GTCTTATGAC TCTGGACGTT 
ATCAACATTC TCAAGTACGG AAGGTCCTTG CTCAGAATTG GCGACAAAAG CGATTGGGGC 
CTTGCAGCTC TCGATCCACA GAGTCGGCTG AACCATCAGA AAAGAACGCT CAACGGCCGT 
CTGGGTCTCC TTGGACGTGA GTTTAAGGAT GAAGAAATAC CCGTCATTAA CGACGCCATC 
CTCAGCCCTG CGACCCCTCT CGATCCAGCT GCAGCTAATG GCTTCGGCCG TCATGACAGT 
GTCGGTTCCG CCCAAACTCA GAACGGTGAG ACGGCCGGGC TTAGTGCACG ACAGCTCAAT 
GTTCTCAAGC GAAAGCGGAA GAAGGAGGCT CAGAAGGCTG CTCAGGGCAA GAGCGGCTTC 
GGTGACCTCT CCTTGCGCCG AAACACAACC GCCGGCTCGG AGGGCTTTGA TGACACGCCG 
ATGGTGGATG CCGACTCCAA GGGCGGCAAG ATGAATGACT ATTTCAGCCT CGATAGGCCC 
GCTGATATTG AAGAGGACAC TAAGGTTGTC AGCGAATACA AGGGGCCCAT TATCCCCATC 
AAGTCCGAGA TTGAGGCCGA CGACAACTTG GAAGGGGTTG AATGGCCATA CGAACGTCTC 
TGCGATTTCC TCAAGGTCGA CCTGTTTGAT CCTCAGTGGG AAACTCGTCA TGGCGCTGCC 
ATGGGCTTGC GCGAGGTCAT CAGGGTCCAT GGCGCTGGCG CGGGAAGATT GATGGCCAAG 
TCTCGATCAG AGAACAACGA GCTCAATCGG AAGTGGCTGG ATGACCTCGC TTGTCGCCTA 
TGCTGCGTTT TGATGCTTGA CAGGTTCACA GATTATAGTT CTGATACCTC TGTGGCACCT 
ATTCGTGAGA CCGTCGGCCA GACACTTGGA TCCGTCCTTC GTCATTGCCC TCCGGCAACG 
GTCCATGCTA TTTACGAACT GCTGTTCCGC ATGGTGATGC AGAAAGATCT TCAGCTCAAC 
CACCATGAGT GGGCCATTTG CCACGGCGGC ATGATTGGCC TTCGTTATGT TGTTGCTGTG 
AGGAAGGACC TGCTCCTGCA GGATACCGCC ATGATTGATG GCGTTATTCT TGCTGTCATG 
GAAGGATTGG CCAACCAGGA CGACGATGTT CGCTCGGTCA GCGCGGCGAC CCTGATACCC 
ATGGCCAAGG AGTTTGTTAA GATGCGCTCG GATGCCCTTC CTGATCTCAT CAACATCGTT 
TGGGAAAGTC TCTCTAACCT TGGCGACGAT CTCAGTGCGT CCACAGGCAA GATCATGGAC 
CTCTTGGCGA CTCTTTGCAG TTTCCCAGAA GTCTTGGAAA CCATGAAGCA CTCGGCGTCG 
CACGATGAGG AGCGGTCGTT TACACTCCTT GTCCCGAGGC TTTACCCCTT CCTTCGTCAT 
ACCATTACCT CTGTCAGGCT TGCTGTCCTC AAGGCTTTGA TGACCTTCGC CAAGCTCGGA 
GGCGACACAA CGCAAGGATG GCTTGGCGGT AGGATTCTGC GTTTAATCTT CCAAAACATC 
CTTGTCGAGA GGGATCAGGA AACACTGAAT GCATCTGTCG ATCTTTGGGC AGCCCTCGTC 
CGTACACTTG CTAACAACAA CCCTGCGATA CTCGCTGATG AGTTTGGACC ACATGTGGAC 
ACGCTCATGC AGCTGACGCT CCATCCTATC GGTGTGTCTA GAAACCCCAT TCCCATGAAT 
GCGAGCTTGT TCCAGAAGCC TTCTGGCGGA ACCTATTCCA TGCCAGGGCT TGTCGCGGGT 
GGTAACAGGA GGTCATCGCC ACCCGAAGGC GCGGACAGGA CTGTCAAACG GAGGAGAAAA 
TCGACCAAGG TCGACGAGCC TCCTCCAACT TCCCATACTC ACGATGTCGA TGGCCACATG 
ATGCAGGGAG ATGTCGACCT CGTTGGCATG GATGTTCTGA TCCGCTCCCG CGTCTCGGCA 
GCCAAGGCCA TGGGCTTAGT CATGGCCCTG GTCCCACCAT CAACCGTTGA AGCCTACGAT 
GCCAGTATCA TCCCGGCCCT CTCATCCGCA TACTCTTCAA CTCAGCTTAC TGCCAGCATT 
GTCATTGACG AGTACGCCAA GAACAGCGCC TCCAGTCAGG CATCAACTCG TTTCGTCCAA 
CCCTTGCTCG CAATTATTGA GCAAGAACGG CCATCCCACT ACCGAGACTT GGTTACCTAT 
GTGCAGCGAG TACGGTCACA ATCGCAACAG CTGCTTAACC TTTGCCGAGA TCATGGCAAG 
GTTTCGCAGA GCAAGTTGCC CACTCTTGCT GTCGTGGTAC AGGGCGAGCC CGAAGCTGGT 
CCCGGCGCTT TCTCTATTGC GAACGCTGAG AAGGTTGTCA ACGAAGATTT TGAGAGGCTC 
AAAAAGATCA TGGCTCCTGG CCAGCGGCTG ATTGCTGTCA AGGACCTCAA TGATGCCCGC 
GATCTCGTCG TTACTGCTAT TGAGGAGGCC AAGGCGGCAA AGGAAGCTCG CGATACTCGG 
ATCCGTGCTG CAGCAGCATG CGCCTTGGTA TCCATGGAGG TGCTACCCAA GAAACCCAGC 
CCACTCATCA AGGCTATCAT GGATAGCGTC AAGGGTGAGG AGAACAATCT GCTTCAAGGT 
CGCTCGGCTA CCAGCGTCGC GCGTCTGGTA CAGCTCTTCA CCGAGTCTGG CCGCCGCGGC 
CCTGGTGACA AGATTGTTGC CAACTTGGTC AAGTATACTT GCGTGGAAAC TGCGGAAACC 
CCCGAGTTCC CTCAGCACGC TCACAAGACA AACGTTATTC TTTCCATGTC CAAAGAGGAG 
GACAGAGCCG AGCACGAGGA TCCAATTAAA TACGCGCTCG AAGCAAAGAA AGCTCACATT 
ACTCGGCGCG GTGCCAAGCA GGCTCTGGAG GTGCTGTCGC AGACATACGG ACCTGAGCTT 
TTGAGCCGGG TGCCTAGTCT CCGCACCTTC ATGGAGGAGC CTTTGGTCAG AGCCTTCTCG 
GGTGAACTCC CAGCAGAGGC CAGAGATCCT TCGAGCACGT TTGGTCAAGA GATTGTGGAT 
GCCCTGTCCA TCATTCGCAC CATGACCCCA ACACTCCACA CCGCACTTCA CTCATTCGTC 
ATGCAACAAG TGCCCTTTGT CATCAAGGCT CTCCAGTCCC AGCTTTCGGT CTTCCGATAC 
ATGGCTGCCA AGTGTATGGC CACCATCTGC TCCGTCATCA CTGTCGATGG TATGACCGCA 
CTTGTGGAAA AGGTTCTGCC CTCAATCAAC AACCCTGTCG ATCTCAACTT CCGCCAGGGC 
GCCATTGAGG TTATCTATCA TTTGATTGCA GTCATGGGAG ACGCCATCCT GCCCTATGTG 
ATTTTCCTGA TCGTGCCTGT CCTTGGTCGT ATGAGCGATT CCGATGACAA CATCCGTCTT 
ATTGCGACCA CATCATTCGC TACTCTTGTC AAGCTCGTAC CTCTTGAGGC TGGAATTCCT 
GATCCTCCCG GTCTTTCAGA GGAGCTCTTG CGTGGCCGAG ACCGTGAACG AACGTTCATT 
CAGCAGCTGC TTGACCCGAA GAAGGTCGAG CCGTTCCAGA TTCCTGTAGC CATCAAGGCA 
GAACTGCGTT CTTACCAGCA GGAAGGTGTC AACTGGCTCC ATTTCCTCAA CAAATATCAC 
CTCCATGGTA TTCTCTGCGA CGACATGGGC CTCGGCAAGA CGCTTCAGAC AATTTGCATT 
GTCGCCAGTG ACCATCATCA GCGTGCTGAA GAGTTTGCCA AGACCGGTGC GCCAGATGTC 
AGAAGGTTGC CGTCTTTGAT CGTCTGCCCG CCCACCCTTT CAGGACATTG GCAGCAGGAG 
ATCAAGAACT ACGCTCCATT CCTCAGCGTT ACGACCTACG TTGGTCCACC TGCTGAGCGA 
AAGGCTATGA AAGACACTCT GGACAAGACC GACATTGTCA TCACCTCCTA TGATGTGTGC 
CGTAATGACA TCGATGTCAT CAAGAAATAC AGTTGGAACT ACGTGGTGCT TGATGAGGGA 
CACTTGATCA AGAACCCCAA AGCAAAGATC ACCATCGCCG TCAAGCAACT GGCCAGCAAC 
CACCGTCTTA TCCTCACCGG TACACCGATC CAGAACAATG TGCTAGAATT ATGGTCTTTG 
TTTGACTTCC TCATGCCCGG CTTCTTGGGT GCAGAAAAGG TGTTCCTTGA CAGATTCGCG 
AAGCCCATTG CCAACAGTCG ATATAGCAAG GCGTCCTCGA AGGAACAAGA AGCAGGCGCT 
CTGGCTATTG AGGCGCTGCA TAAGCAGGTC CTGCCCTTCC TTTTGCGACG TCTCAAGGAG 
GAAGTTCTTA ACGACTTGCC ACCCAAGATT TTGCAGAACT ACTATTGCGA CCTCAGCGAC 
CTTCAACGCA AACTTTTTGA GGACTTCACC AAGAGGGAGG CGAAGAAGAT CACCGAGGAG 
GCGGGCCGTG ATGACAAGGA AGCCAAGGCC CATATTTTCC AAGCTCTGCA GTACATGCGC 
AAGCTTTGCA ACTCTCCTGC GCTCGTCATG AAGCCCGGTC ACAAGCAGTA CGATGATACA 
CAGAAATTCC TGGCCAAACG AAACACGAGC CTCGAGGATC CGGTCCATGC CCCCAAACTC 
ACGGCACTCC GAGACTTGCT TGTCGACTGC GGTATCGGTG TTGAAGGTCA AGAATCGTCC 
GATCCGCTCT ACACGCCCAT TAAGCCTCAT CGTGCACTCA TCTTCTGCCA GATGAAGGAA 
ATGTTGGATA TGGTTCAGAA TACCGTCCTC AAGACTATGC TTCCCTCGGT ATCTCACCTT 
CGTCTTGACG GATCCGTCGA CCCTACCCGC CGTCAGGACA TTGTCAACAA GTTCAACAGT 
GACCCATCAT ACGATGTGTT GCTGCTCACC ACGAGCGTCG GTGGTCTTGG TCTCAACCTG 
ACTGGTGCAG ATACTGTTAT CTTTGTTGAG CACGATTGGA ACCCGCAGAA GGATCTGCAG 
GCCATGGACC GCGCCCATCG TATCGGTCAG AAGAAGGTGG TCAACGTTTA CCGCTTGATC 
ACCCGCGGAA CACTTGAGGA GAAGATTCTC AGTCTTCAAC GTTTCAAGAT CGACGTCGCC 
AGTACGGTAG TGAACCAGCA GAATGCAGGA CTTGCGACCA TGGATACGGA TCAGATTCTT 
GATCTGTTCA ACCTTGGTGA TGCCGGACCA AACCTCATAA CGGAGGGCAA GAACAAGGAC 
AACCTCGAGG GCAGGGAGGA GGACATGGTG GATATCGAGA CGGGCGATGT CAGGCAACCT 
GGCAAGAAAG CGGCTTGGCT CGAAGGCCTC GGGGAGCTGT GGGATAATTC ACAGTACGAG 
GAAAGCTTCG ATCTCGACGG CTTCCTGAAG ACTATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crf8-1 ( XM_011395636.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393938.1 Neurospora crassa OR74A TBP associated factor (crf8-1), mRNA.

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CloneID	ONf00567
Clone ID Related Accession (Same CDS sequence)	XM_011395637.1 , XM_011395636.1
Accession Version	XM_011395636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	TBP associated factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503).

ATGGCGTCTC GGCTAGATCG CCTTGTCACG CTTATCGAGA CGGGCAGTAC TCGCCTGATT 
CGGGATGCTG CCGTAGATCA GCTCGCCACA TGGCAAAAGG AACACCCCGA TGAGCTTTTC 
AATCTACTCT CTCGGGTTGT CCCCTACTTG CGCCACAAAG ACTGGGAAAC CAGATCAACT 
GCTGCCAAGG CTATAGGCAA AATCATCGAA AATGCACCGC AGTATGATCC CAATGCGGAC 
GAGGAACCCG ACGCGGCGAT GAAAGATGAA GATAGCAATA GCTTCATCAA GAAAGAAGAG 
GGCCCCCCAA AGCTCTCTCT TAGTGAGGAG GAGCAGTTCA GTCTTATGAC TCTGGACGTT 
ATCAACATTC TCAAGTACGG AAGGTCCTTG CTCAGAATTG GCGACAAAAG CGATTGGGGC 
CTTGCAGCTC TCGATCCACA GAGTCGGCTG AACCATCAGA AAAGAACGCT CAACGGCCGT 
CTGGGTCTCC TTGGACGTGA GTTTAAGGAT GAAGAAATAC CCGTCATTAA CGACGCCATC 
CTCAGCCCTG CGACCCCTCT CGATCCAGCT GCAGCTAATG GCTTCGGCCG TCATGACAGT 
GTCGGTTCCG CCCAAACTCA GAACGGTGAG ACGGCCGGGC TTAGTGCACG ACAGCTCAAT 
GTTCTCAAGC GAAAGCGGAA GAAGGAGGCT CAGAAGGCTG CTCAGGGCAA GAGCGGCTTC 
GGTGACCTCT CCTTGCGCCG AAACACAACC GCCGGCTCGG AGGGCTTTGA TGACACGCCG 
ATGGTGGATG CCGACTCCAA GGGCGGCAAG ATGAATGACT ATTTCAGCCT CGATAGGCCC 
GCTGATATTG AAGAGGACAC TAAGGTTGTC AGCGAATACA AGGGGCCCAT TATCCCCATC 
AAGTCCGAGA TTGAGGCCGA CGACAACTTG GAAGGGGTTG AATGGCCATA CGAACGTCTC 
TGCGATTTCC TCAAGGTCGA CCTGTTTGAT CCTCAGTGGG AAACTCGTCA TGGCGCTGCC 
ATGGGCTTGC GCGAGGTCAT CAGGGTCCAT GGCGCTGGCG CGGGAAGATT GATGGCCAAG 
TCTCGATCAG AGAACAACGA GCTCAATCGG AAGTGGCTGG ATGACCTCGC TTGTCGCCTA 
TGCTGCGTTT TGATGCTTGA CAGGTTCACA GATTATAGTT CTGATACCTC TGTGGCACCT 
ATTCGTGAGA CCGTCGGCCA GACACTTGGA TCCGTCCTTC GTCATTGCCC TCCGGCAACG 
GTCCATGCTA TTTACGAACT GCTGTTCCGC ATGGTGATGC AGAAAGATCT TCAGCTCAAC 
CACCATGAGT GGGCCATTTG CCACGGCGGC ATGATTGGCC TTCGTTATGT TGTTGCTGTG 
AGGAAGGACC TGCTCCTGCA GGATACCGCC ATGATTGATG GCGTTATTCT TGCTGTCATG 
GAAGGATTGG CCAACCAGGA CGACGATGTT CGCTCGGTCA GCGCGGCGAC CCTGATACCC 
ATGGCCAAGG AGTTTGTTAA GATGCGCTCG GATGCCCTTC CTGATCTCAT CAACATCGTT 
TGGGAAAGTC TCTCTAACCT TGGCGACGAT CTCAGTGCGT CCACAGGCAA GATCATGGAC 
CTCTTGGCGA CTCTTTGCAG TTTCCCAGAA GTCTTGGAAA CCATGAAGCA CTCGGCGTCG 
CACGATGAGG AGCGGTCGTT TACACTCCTT GTCCCGAGGC TTTACCCCTT CCTTCGTCAT 
ACCATTACCT CTGTCAGGCT TGCTGTCCTC AAGGCTTTGA TGACCTTCGC CAAGCTCGGA 
GGCGACACAA CGCAAGGATG GCTTGGCGGT AGGATTCTGC GTTTAATCTT CCAAAACATC 
CTTGTCGAGA GGGATCAGGA AACACTGAAT GCATCTGTCG ATCTTTGGGC AGCCCTCGTC 
CGTACACTTG CTAACAACAA CCCTGCGATA CTCGCTGATG AGTTTGGACC ACATGTGGAC 
ACGCTCATGC AGCTGACGCT CCATCCTATC GGTGTGTCTA GAAACCCCAT TCCCATGAAT 
GCGAGCTTGT TCCAGAAGCC TTCTGGCGGA ACCTATTCCA TGCCAGGGCT TGTCGCGGGT 
GGTAACAGGA GGTCATCGCC ACCCGAAGGC GCGGACAGGA CTGTCAAACG GAGGAGAAAA 
TCGACCAAGG TCGACGAGCC TCCTCCAACT TCCCATACTC ACGATGTCGA TGGCCACATG 
ATGCAGGGAG ATGTCGACCT CGTTGGCATG GATGTTCTGA TCCGCTCCCG CGTCTCGGCA 
GCCAAGGCCA TGGGCTTAGT CATGGCCCTG GTCCCACCAT CAACCGTTGA AGCCTACGAT 
GCCAGTATCA TCCCGGCCCT CTCATCCGCA TACTCTTCAA CTCAGCTTAC TGCCAGCATT 
GTCATTGACG AGTACGCCAA GAACAGCGCC TCCAGTCAGG CATCAACTCG TTTCGTCCAA 
CCCTTGCTCG CAATTATTGA GCAAGAACGG CCATCCCACT ACCGAGACTT GGTTACCTAT 
GTGCAGCGAG TACGGTCACA ATCGCAACAG CTGCTTAACC TTTGCCGAGA TCATGGCAAG 
GTTTCGCAGA GCAAGTTGCC CACTCTTGCT GTCGTGGTAC AGGGCGAGCC CGAAGCTGGT 
CCCGGCGCTT TCTCTATTGC GAACGCTGAG AAGGTTGTCA ACGAAGATTT TGAGAGGCTC 
AAAAAGATCA TGGCTCCTGG CCAGCGGCTG ATTGCTGTCA AGGACCTCAA TGATGCCCGC 
GATCTCGTCG TTACTGCTAT TGAGGAGGCC AAGGCGGCAA AGGAAGCTCG CGATACTCGG 
ATCCGTGCTG CAGCAGCATG CGCCTTGGTA TCCATGGAGG TGCTACCCAA GAAACCCAGC 
CCACTCATCA AGGCTATCAT GGATAGCGTC AAGGGTGAGG AGAACAATCT GCTTCAAGGT 
CGCTCGGCTA CCAGCGTCGC GCGTCTGGTA CAGCTCTTCA CCGAGTCTGG CCGCCGCGGC 
CCTGGTGACA AGATTGTTGC CAACTTGGTC AAGTATACTT GCGTGGAAAC TGCGGAAACC 
CCCGAGTTCC CTCAGCACGC TCACAAGACA AACGTTATTC TTTCCATGTC CAAAGAGGAG 
GACAGAGCCG AGCACGAGGA TCCAATTAAA TACGCGCTCG AAGCAAAGAA AGCTCACATT 
ACTCGGCGCG GTGCCAAGCA GGCTCTGGAG GTGCTGTCGC AGACATACGG ACCTGAGCTT 
TTGAGCCGGG TGCCTAGTCT CCGCACCTTC ATGGAGGAGC CTTTGGTCAG AGCCTTCTCG 
GGTGAACTCC CAGCAGAGGC CAGAGATCCT TCGAGCACGT TTGGTCAAGA GATTGTGGAT 
GCCCTGTCCA TCATTCGCAC CATGACCCCA ACACTCCACA CCGCACTTCA CTCATTCGTC 
ATGCAACAAG TGCCCTTTGT CATCAAGGCT CTCCAGTCCC AGCTTTCGGT CTTCCGATAC 
ATGGCTGCCA AGTGTATGGC CACCATCTGC TCCGTCATCA CTGTCGATGG TATGACCGCA 
CTTGTGGAAA AGGTTCTGCC CTCAATCAAC AACCCTGTCG ATCTCAACTT CCGCCAGGGC 
GCCATTGAGG TTATCTATCA TTTGATTGCA GTCATGGGAG ACGCCATCCT GCCCTATGTG 
ATTTTCCTGA TCGTGCCTGT CCTTGGTCGT ATGAGCGATT CCGATGACAA CATCCGTCTT 
ATTGCGACCA CATCATTCGC TACTCTTGTC AAGCTCGTAC CTCTTGAGGC TGGAATTCCT 
GATCCTCCCG GTCTTTCAGA GGAGCTCTTG CGTGGCCGAG ACCGTGAACG AACGTTCATT 
CAGCAGCTGC TTGACCCGAA GAAGGTCGAG CCGTTCCAGA TTCCTGTAGC CATCAAGGCA 
GAACTGCGTT CTTACCAGCA GGAAGGTGTC AACTGGCTCC ATTTCCTCAA CAAATATCAC 
CTCCATGGTA TTCTCTGCGA CGACATGGGC CTCGGCAAGA CGCTTCAGAC AATTTGCATT 
GTCGCCAGTG ACCATCATCA GCGTGCTGAA GAGTTTGCCA AGACCGGTGC GCCAGATGTC 
AGAAGGTTGC CGTCTTTGAT CGTCTGCCCG CCCACCCTTT CAGGACATTG GCAGCAGGAG 
ATCAAGAACT ACGCTCCATT CCTCAGCGTT ACGACCTACG TTGGTCCACC TGCTGAGCGA 
AAGGCTATGA AAGACACTCT GGACAAGACC GACATTGTCA TCACCTCCTA TGATGTGTGC 
CGTAATGACA TCGATGTCAT CAAGAAATAC AGTTGGAACT ACGTGGTGCT TGATGAGGGA 
CACTTGATCA AGAACCCCAA AGCAAAGATC ACCATCGCCG TCAAGCAACT GGCCAGCAAC 
CACCGTCTTA TCCTCACCGG TACACCGATC CAGAACAATG TGCTAGAATT ATGGTCTTTG 
TTTGACTTCC TCATGCCCGG CTTCTTGGGT GCAGAAAAGG TGTTCCTTGA CAGATTCGCG 
AAGCCCATTG CCAACAGTCG ATATAGCAAG GCGTCCTCGA AGGAACAAGA AGCAGGCGCT 
CTGGCTATTG AGGCGCTGCA TAAGCAGGTC CTGCCCTTCC TTTTGCGACG TCTCAAGGAG 
GAAGTTCTTA ACGACTTGCC ACCCAAGATT TTGCAGAACT ACTATTGCGA CCTCAGCGAC 
CTTCAACGCA AACTTTTTGA GGACTTCACC AAGAGGGAGG CGAAGAAGAT CACCGAGGAG 
GCGGGCCGTG ATGACAAGGA AGCCAAGGCC CATATTTTCC AAGCTCTGCA GTACATGCGC 
AAGCTTTGCA ACTCTCCTGC GCTCGTCATG AAGCCCGGTC ACAAGCAGTA CGATGATACA 
CAGAAATTCC TGGCCAAACG AAACACGAGC CTCGAGGATC CGGTCCATGC CCCCAAACTC 
ACGGCACTCC GAGACTTGCT TGTCGACTGC GGTATCGGTG TTGAAGGTCA AGAATCGTCC 
GATCCGCTCT ACACGCCCAT TAAGCCTCAT CGTGCACTCA TCTTCTGCCA GATGAAGGAA 
ATGTTGGATA TGGTTCAGAA TACCGTCCTC AAGACTATGC TTCCCTCGGT ATCTCACCTT 
CGTCTTGACG GATCCGTCGA CCCTACCCGC CGTCAGGACA TTGTCAACAA GTTCAACAGT 
GACCCATCAT ACGATGTGTT GCTGCTCACC ACGAGCGTCG GTGGTCTTGG TCTCAACCTG 
ACTGGTGCAG ATACTGTTAT CTTTGTTGAG CACGATTGGA ACCCGCAGAA GGATCTGCAG 
GCCATGGACC GCGCCCATCG TATCGGTCAG AAGAAGGTGG TCAACGTTTA CCGCTTGATC 
ACCCGCGGAA CACTTGAGGA GAAGATTCTC AGTCTTCAAC GTTTCAAGAT CGACGTCGCC 
AGTACGGTAG TGAACCAGCA GAATGCAGGA CTTGCGACCA TGGATACGGA TCAGATTCTT 
GATCTGTTCA ACCTTGGTGA TGCCGGACCA AACCTCATAA CGGAGGGCAA GAACAAGGAC 
AACCTCGAGG GCAGGGAGGA GGACATGGTG GATATCGAGA CGGGCGATGT CAGGCAACCT 
GGCAAGAAAG CGGCTTGGCT CGAAGGCCTC GGGGAGCTGT GGGATAATTC ACAGTACGAG 
GAAAGCTTCG ATCTCGACGG CTTCCTGAAG ACTATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393938.1
CDS	777..6458
Translation	MASRLDRLVTLIETGSTRLIRDAAVDQLATWQKEHPDELFNLLSRVVPYLRHKDWETRSTAAKAIGKIIENAPQYDPNADEEPDAAMKDEDSNSFIKKEEGPPKLSLSEEEQFSLMTLDVINILKYGRSLLRIGDKSDWGLAALDPQSRLNHQKRTLNGRLGLLGREFKDEEIPVINDAILSPATPLDPAAANGFGRHDSVGSAQTQNGETAGLSARQLNVLKRKRKKEAQKAAQGKSGFGDLSLRRNTTAGSEGFDDTPMVDADSKGGKMNDYFSLDRPADIEEDTKVVSEYKGPIIPIKSEIEADDNLEGVEWPYERLCDFLKVDLFDPQWETRHGAAMGLREVIRVHGAGAGRLMAKSRSENNELNRKWLDDLACRLCCVLMLDRFTDYSSDTSVAPIRETVGQTLGSVLRHCPPATVHAIYELLFRMVMQKDLQLNHHEWAICHGGMIGLRYVVAVRKDLLLQDTAMIDGVILAVMEGLANQDDDVRSVSAATLIPMAKEFVKMRSDALPDLINIVWESLSNLGDDLSASTGKIMDLLATLCSFPEVLETMKHSASHDEERSFTLLVPRLYPFLRHTITSVRLAVLKALMTFAKLGGDTTQGWLGGRILRLIFQNILVERDQETLNASVDLWAALVRTLANNNPAILADEFGPHVDTLMQLTLHPIGVSRNPIPMNASLFQKPSGGTYSMPGLVAGGNRRSSPPEGADRTVKRRRKSTKVDEPPPTSHTHDVDGHMMQGDVDLVGMDVLIRSRVSAAKAMGLVMALVPPSTVEAYDASIIPALSSAYSSTQLTASIVIDEYAKNSASSQASTRFVQPLLAIIEQERPSHYRDLVTYVQRVRSQSQQLLNLCRDHGKVSQSKLPTLAVVVQGEPEAGPGAFSIANAEKVVNEDFERLKKIMAPGQRLIAVKDLNDARDLVVTAIEEAKAAKEARDTRIRAAAACALVSMEVLPKKPSPLIKAIMDSVKGEENNLLQGRSATSVARLVQLFTESGRRGPGDKIVANLVKYTCVETAETPEFPQHAHKTNVILSMSKEEDRAEHEDPIKYALEAKKAHITRRGAKQALEVLSQTYGPELLSRVPSLRTFMEEPLVRAFSGELPAEARDPSSTFGQEIVDALSIIRTMTPTLHTALHSFVMQQVPFVIKALQSQLSVFRYMAAKCMATICSVITVDGMTALVEKVLPSINNPVDLNFRQGAIEVIYHLIAVMGDAILPYVIFLIVPVLGRMSDSDDNIRLIATTSFATLVKLVPLEAGIPDPPGLSEELLRGRDRERTFIQQLLDPKKVEPFQIPVAIKAELRSYQQEGVNWLHFLNKYHLHGILCDDMGLGKTLQTICIVASDHHQRAEEFAKTGAPDVRRLPSLIVCPPTLSGHWQQEIKNYAPFLSVTTYVGPPAERKAMKDTLDKTDIVITSYDVCRNDIDVIKKYSWNYVVLDEGHLIKNPKAKITIAVKQLASNHRLILTGTPIQNNVLELWSLFDFLMPGFLGAEKVFLDRFAKPIANSRYSKASSKEQEAGALAIEALHKQVLPFLLRRLKEEVLNDLPPKILQNYYCDLSDLQRKLFEDFTKREAKKITEEAGRDDKEAKAHIFQALQYMRKLCNSPALVMKPGHKQYDDTQKFLAKRNTSLEDPVHAPKLTALRDLLVDCGIGVEGQESSDPLYTPIKPHRALIFCQMKEMLDMVQNTVLKTMLPSVSHLRLDGSVDPTRRQDIVNKFNSDPSYDVLLLTTSVGGLGLNLTGADTVIFVEHDWNPQKDLQAMDRAHRIGQKKVVNVYRLITRGTLEEKILSLQRFKIDVASTVVNQQNAGLATMDTDQILDLFNLGDAGPNLITEGKNKDNLEGREEDMVDIETGDVRQPGKKAAWLEGLGELWDNSQYEESFDLDGFLKTMQ

Target ORF information:

RefSeq Version	XM_011395636.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A TBP associated factor (crf8-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395636.1

ORF Insert Sequence:

ATGGCGTCTC GGCTAGATCG CCTTGTCACG CTTATCGAGA CGGGCAGTAC TCGCCTGATT 
CGGGATGCTG CCGTAGATCA GCTCGCCACA TGGCAAAAGG AACACCCCGA TGAGCTTTTC 
AATCTACTCT CTCGGGTTGT CCCCTACTTG CGCCACAAAG ACTGGGAAAC CAGATCAACT 
GCTGCCAAGG CTATAGGCAA AATCATCGAA AATGCACCGC AGTATGATCC CAATGCGGAC 
GAGGAACCCG ACGCGGCGAT GAAAGATGAA GATAGCAATA GCTTCATCAA GAAAGAAGAG 
GGCCCCCCAA AGCTCTCTCT TAGTGAGGAG GAGCAGTTCA GTCTTATGAC TCTGGACGTT 
ATCAACATTC TCAAGTACGG AAGGTCCTTG CTCAGAATTG GCGACAAAAG CGATTGGGGC 
CTTGCAGCTC TCGATCCACA GAGTCGGCTG AACCATCAGA AAAGAACGCT CAACGGCCGT 
CTGGGTCTCC TTGGACGTGA GTTTAAGGAT GAAGAAATAC CCGTCATTAA CGACGCCATC 
CTCAGCCCTG CGACCCCTCT CGATCCAGCT GCAGCTAATG GCTTCGGCCG TCATGACAGT 
GTCGGTTCCG CCCAAACTCA GAACGGTGAG ACGGCCGGGC TTAGTGCACG ACAGCTCAAT 
GTTCTCAAGC GAAAGCGGAA GAAGGAGGCT CAGAAGGCTG CTCAGGGCAA GAGCGGCTTC 
GGTGACCTCT CCTTGCGCCG AAACACAACC GCCGGCTCGG AGGGCTTTGA TGACACGCCG 
ATGGTGGATG CCGACTCCAA GGGCGGCAAG ATGAATGACT ATTTCAGCCT CGATAGGCCC 
GCTGATATTG AAGAGGACAC TAAGGTTGTC AGCGAATACA AGGGGCCCAT TATCCCCATC 
AAGTCCGAGA TTGAGGCCGA CGACAACTTG GAAGGGGTTG AATGGCCATA CGAACGTCTC 
TGCGATTTCC TCAAGGTCGA CCTGTTTGAT CCTCAGTGGG AAACTCGTCA TGGCGCTGCC 
ATGGGCTTGC GCGAGGTCAT CAGGGTCCAT GGCGCTGGCG CGGGAAGATT GATGGCCAAG 
TCTCGATCAG AGAACAACGA GCTCAATCGG AAGTGGCTGG ATGACCTCGC TTGTCGCCTA 
TGCTGCGTTT TGATGCTTGA CAGGTTCACA GATTATAGTT CTGATACCTC TGTGGCACCT 
ATTCGTGAGA CCGTCGGCCA GACACTTGGA TCCGTCCTTC GTCATTGCCC TCCGGCAACG 
GTCCATGCTA TTTACGAACT GCTGTTCCGC ATGGTGATGC AGAAAGATCT TCAGCTCAAC 
CACCATGAGT GGGCCATTTG CCACGGCGGC ATGATTGGCC TTCGTTATGT TGTTGCTGTG 
AGGAAGGACC TGCTCCTGCA GGATACCGCC ATGATTGATG GCGTTATTCT TGCTGTCATG 
GAAGGATTGG CCAACCAGGA CGACGATGTT CGCTCGGTCA GCGCGGCGAC CCTGATACCC 
ATGGCCAAGG AGTTTGTTAA GATGCGCTCG GATGCCCTTC CTGATCTCAT CAACATCGTT 
TGGGAAAGTC TCTCTAACCT TGGCGACGAT CTCAGTGCGT CCACAGGCAA GATCATGGAC 
CTCTTGGCGA CTCTTTGCAG TTTCCCAGAA GTCTTGGAAA CCATGAAGCA CTCGGCGTCG 
CACGATGAGG AGCGGTCGTT TACACTCCTT GTCCCGAGGC TTTACCCCTT CCTTCGTCAT 
ACCATTACCT CTGTCAGGCT TGCTGTCCTC AAGGCTTTGA TGACCTTCGC CAAGCTCGGA 
GGCGACACAA CGCAAGGATG GCTTGGCGGT AGGATTCTGC GTTTAATCTT CCAAAACATC 
CTTGTCGAGA GGGATCAGGA AACACTGAAT GCATCTGTCG ATCTTTGGGC AGCCCTCGTC 
CGTACACTTG CTAACAACAA CCCTGCGATA CTCGCTGATG AGTTTGGACC ACATGTGGAC 
ACGCTCATGC AGCTGACGCT CCATCCTATC GGTGTGTCTA GAAACCCCAT TCCCATGAAT 
GCGAGCTTGT TCCAGAAGCC TTCTGGCGGA ACCTATTCCA TGCCAGGGCT TGTCGCGGGT 
GGTAACAGGA GGTCATCGCC ACCCGAAGGC GCGGACAGGA CTGTCAAACG GAGGAGAAAA 
TCGACCAAGG TCGACGAGCC TCCTCCAACT TCCCATACTC ACGATGTCGA TGGCCACATG 
ATGCAGGGAG ATGTCGACCT CGTTGGCATG GATGTTCTGA TCCGCTCCCG CGTCTCGGCA 
GCCAAGGCCA TGGGCTTAGT CATGGCCCTG GTCCCACCAT CAACCGTTGA AGCCTACGAT 
GCCAGTATCA TCCCGGCCCT CTCATCCGCA TACTCTTCAA CTCAGCTTAC TGCCAGCATT 
GTCATTGACG AGTACGCCAA GAACAGCGCC TCCAGTCAGG CATCAACTCG TTTCGTCCAA 
CCCTTGCTCG CAATTATTGA GCAAGAACGG CCATCCCACT ACCGAGACTT GGTTACCTAT 
GTGCAGCGAG TACGGTCACA ATCGCAACAG CTGCTTAACC TTTGCCGAGA TCATGGCAAG 
GTTTCGCAGA GCAAGTTGCC CACTCTTGCT GTCGTGGTAC AGGGCGAGCC CGAAGCTGGT 
CCCGGCGCTT TCTCTATTGC GAACGCTGAG AAGGTTGTCA ACGAAGATTT TGAGAGGCTC 
AAAAAGATCA TGGCTCCTGG CCAGCGGCTG ATTGCTGTCA AGGACCTCAA TGATGCCCGC 
GATCTCGTCG TTACTGCTAT TGAGGAGGCC AAGGCGGCAA AGGAAGCTCG CGATACTCGG 
ATCCGTGCTG CAGCAGCATG CGCCTTGGTA TCCATGGAGG TGCTACCCAA GAAACCCAGC 
CCACTCATCA AGGCTATCAT GGATAGCGTC AAGGGTGAGG AGAACAATCT GCTTCAAGGT 
CGCTCGGCTA CCAGCGTCGC GCGTCTGGTA CAGCTCTTCA CCGAGTCTGG CCGCCGCGGC 
CCTGGTGACA AGATTGTTGC CAACTTGGTC AAGTATACTT GCGTGGAAAC TGCGGAAACC 
CCCGAGTTCC CTCAGCACGC TCACAAGACA AACGTTATTC TTTCCATGTC CAAAGAGGAG 
GACAGAGCCG AGCACGAGGA TCCAATTAAA TACGCGCTCG AAGCAAAGAA AGCTCACATT 
ACTCGGCGCG GTGCCAAGCA GGCTCTGGAG GTGCTGTCGC AGACATACGG ACCTGAGCTT 
TTGAGCCGGG TGCCTAGTCT CCGCACCTTC ATGGAGGAGC CTTTGGTCAG AGCCTTCTCG 
GGTGAACTCC CAGCAGAGGC CAGAGATCCT TCGAGCACGT TTGGTCAAGA GATTGTGGAT 
GCCCTGTCCA TCATTCGCAC CATGACCCCA ACACTCCACA CCGCACTTCA CTCATTCGTC 
ATGCAACAAG TGCCCTTTGT CATCAAGGCT CTCCAGTCCC AGCTTTCGGT CTTCCGATAC 
ATGGCTGCCA AGTGTATGGC CACCATCTGC TCCGTCATCA CTGTCGATGG TATGACCGCA 
CTTGTGGAAA AGGTTCTGCC CTCAATCAAC AACCCTGTCG ATCTCAACTT CCGCCAGGGC 
GCCATTGAGG TTATCTATCA TTTGATTGCA GTCATGGGAG ACGCCATCCT GCCCTATGTG 
ATTTTCCTGA TCGTGCCTGT CCTTGGTCGT ATGAGCGATT CCGATGACAA CATCCGTCTT 
ATTGCGACCA CATCATTCGC TACTCTTGTC AAGCTCGTAC CTCTTGAGGC TGGAATTCCT 
GATCCTCCCG GTCTTTCAGA GGAGCTCTTG CGTGGCCGAG ACCGTGAACG AACGTTCATT 
CAGCAGCTGC TTGACCCGAA GAAGGTCGAG CCGTTCCAGA TTCCTGTAGC CATCAAGGCA 
GAACTGCGTT CTTACCAGCA GGAAGGTGTC AACTGGCTCC ATTTCCTCAA CAAATATCAC 
CTCCATGGTA TTCTCTGCGA CGACATGGGC CTCGGCAAGA CGCTTCAGAC AATTTGCATT 
GTCGCCAGTG ACCATCATCA GCGTGCTGAA GAGTTTGCCA AGACCGGTGC GCCAGATGTC 
AGAAGGTTGC CGTCTTTGAT CGTCTGCCCG CCCACCCTTT CAGGACATTG GCAGCAGGAG 
ATCAAGAACT ACGCTCCATT CCTCAGCGTT ACGACCTACG TTGGTCCACC TGCTGAGCGA 
AAGGCTATGA AAGACACTCT GGACAAGACC GACATTGTCA TCACCTCCTA TGATGTGTGC 
CGTAATGACA TCGATGTCAT CAAGAAATAC AGTTGGAACT ACGTGGTGCT TGATGAGGGA 
CACTTGATCA AGAACCCCAA AGCAAAGATC ACCATCGCCG TCAAGCAACT GGCCAGCAAC 
CACCGTCTTA TCCTCACCGG TACACCGATC CAGAACAATG TGCTAGAATT ATGGTCTTTG 
TTTGACTTCC TCATGCCCGG CTTCTTGGGT GCAGAAAAGG TGTTCCTTGA CAGATTCGCG 
AAGCCCATTG CCAACAGTCG ATATAGCAAG GCGTCCTCGA AGGAACAAGA AGCAGGCGCT 
CTGGCTATTG AGGCGCTGCA TAAGCAGGTC CTGCCCTTCC TTTTGCGACG TCTCAAGGAG 
GAAGTTCTTA ACGACTTGCC ACCCAAGATT TTGCAGAACT ACTATTGCGA CCTCAGCGAC 
CTTCAACGCA AACTTTTTGA GGACTTCACC AAGAGGGAGG CGAAGAAGAT CACCGAGGAG 
GCGGGCCGTG ATGACAAGGA AGCCAAGGCC CATATTTTCC AAGCTCTGCA GTACATGCGC 
AAGCTTTGCA ACTCTCCTGC GCTCGTCATG AAGCCCGGTC ACAAGCAGTA CGATGATACA 
CAGAAATTCC TGGCCAAACG AAACACGAGC CTCGAGGATC CGGTCCATGC CCCCAAACTC 
ACGGCACTCC GAGACTTGCT TGTCGACTGC GGTATCGGTG TTGAAGGTCA AGAATCGTCC 
GATCCGCTCT ACACGCCCAT TAAGCCTCAT CGTGCACTCA TCTTCTGCCA GATGAAGGAA 
ATGTTGGATA TGGTTCAGAA TACCGTCCTC AAGACTATGC TTCCCTCGGT ATCTCACCTT 
CGTCTTGACG GATCCGTCGA CCCTACCCGC CGTCAGGACA TTGTCAACAA GTTCAACAGT 
GACCCATCAT ACGATGTGTT GCTGCTCACC ACGAGCGTCG GTGGTCTTGG TCTCAACCTG 
ACTGGTGCAG ATACTGTTAT CTTTGTTGAG CACGATTGGA ACCCGCAGAA GGATCTGCAG 
GCCATGGACC GCGCCCATCG TATCGGTCAG AAGAAGGTGG TCAACGTTTA CCGCTTGATC 
ACCCGCGGAA CACTTGAGGA GAAGATTCTC AGTCTTCAAC GTTTCAAGAT CGACGTCGCC 
AGTACGGTAG TGAACCAGCA GAATGCAGGA CTTGCGACCA TGGATACGGA TCAGATTCTT 
GATCTGTTCA ACCTTGGTGA TGCCGGACCA AACCTCATAA CGGAGGGCAA GAACAAGGAC 
AACCTCGAGG GCAGGGAGGA GGACATGGTG GATATCGAGA CGGGCGATGT CAGGCAACCT 
GGCAAGAAAG CGGCTTGGCT CGAAGGCCTC GGGGAGCTGT GGGATAATTC ACAGTACGAG 
GAAAGCTTCG ATCTCGACGG CTTCCTGAAG ACTATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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crf8-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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