set-2 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	set-2
Entrez Gene ID	3873910
Full Name	set-domain histone methyltransferase-2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_952647.2	XP_957740.1	histone-lysine N-methyltransferase

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SET2	NP_012367.2
Eremothecium gossypii	AGOS_AEL128C	NP_984733.2
Neurospora crassa	NCU00269	XP_957740.1
Magnaporthe oryzae (rice blast fungus)	MGG_01661	XP_003714647.1
Kluyveromyces lactis	KLLA0A06600g	XP_451294.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

loss of histone H3K36 methylation, due to either deletion of SET-2 or H3K36R mutation, results in arrhythmic frq transcription and loss of overt circadian clock rhythmicity
Title: Suppression of WHITE COLLAR-independent frequency Transcription by Histone H3 Lysine 36 Methyltransferase SET-2 Is Necessary for Clock Function in Neurospora.

The following set-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the set-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf04739	XM_952647.2 Latest version!	Neurospora crassa OR74A histone-lysine N-methyltransferase (set-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

set-2 ( XM_952647.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_957740.1 Neurospora crassa OR74A histone-lysine N-methyltransferase (set-2), mRNA.

Price & Availability↑

CloneID	ONf04739
Clone ID Related Accession (Same CDS sequence)	XM_952647.2
Accession Version	XM_952647.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	histone-lysine N-methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_952647.1.

ATGGAGGACG GCCATCACTC ACCGGATTCG ACCAAGGAAT CAAAGTCGGA GGAACACTCC 
AAGATGAACG GCTCCAAGCT AAAACGGGAT GGCGCCAGCA ACGCCGGCAA TGCGGCCACG 
CCAAACGGCT CCCAGACCGG AATCTCGAGA CCCCCAAGCA TGTCGCCCGA CGAGCACAAG 
GCTGCGAGTG AGAGCACAGC AACTCCCAGC GAAAATGTCC CGACACAAAA GCCTTCGCGA 
AAGGCGTCTC AGAAGAACAT GAAGAGGGAA CCGGTGCTCT TCAACCATCT CCCCGACGTG 
AGGGAAGAGG CATGCACACA TTTCCAGGTC ATTCATGACT GTCTCTATGG CTCAAAGAAC 
ATGGGTGCAT CCGAGCACGA TGCGTTGGAT TGTGACTGTG CGGAAGAATG GCGCGGTGAT 
ATGAACCATG CGTGCGGGGA AGATTCAGAC TGTATTAATC GGGCCACGAA AATGGAGTGC 
GTTGACGGCG ATTGCAACTG TGGTAGCGGA TGCCAGAACC AGCGCTTTCA GCGAAAACAG 
TACGCCGACG TATCGGTCAT CAAGACGGAA AAGAAGGGGT TCGGCCTCCG GGCAAACACG 
GATTTGCAAG TTAACGACTT CATTTTCGAG TACATTGGCG AGGTCATCAA CGAGCCGACA 
TTTCGCAGCC GCATGGTCAA ATACGACAAG GAGGGCATCA AGCACTTCTA TTTCATGTCA 
CTAACCAAAT CCGAGTTTGT TGATGCCACT AAGAAGGGCA ACCTCGGCAG GTTCTGCAAC 
CATTCGTGCG ATCCTAATTG CTACGTCGAC AAGTGGGTGG TTGGTGACAA GCTGCGCATG 
GGCATTTTTG CGGGGCGCGC AATCAAGGCT GGTGAGGAAC TGGTGTTCAA CTACAACGTG 
GACCGCTATG GCGCCGACCC GCAGCCTTGC TACTGCGGTG AACCAAACTG CACCGGTTTC 
ATTGGCGGCA AAACGCAAAC AGAGCGTGCA ACCAAACTGC CGCCTGCCAC CATCGAGGCG 
CTTGGTATCG AAGATGGAGA CAGCTGGGAC ACGGCGGTGG CGGCCACCGT CAAGAAGCCC 
CGCAAGAAGA AGGCTACCGA GGACGATGAA GAGTACATCA ACCGCTTCGA GCCTCGTGGC 
CTCGATGAAG AGGGTGTCAC CAAGGTCATG GCCACGCTCA TGCAGTGCAA GGAGAAATGG 
ATTGCCGTCA AGCTGCTCGG CCGCCTTCAG AATGCCGACG ATGACCATGT CCGGAACCGG 
GTTGTTAAGA TGCACGGATA CCAGATTTTG AAGACAACCC TCAACACCTT TAAGGAAGAC 
ACCAATGTCG TACTGCAGAT TCTCGATATC CTGTACCAAC TCCCCCGCAT CACCAAGAAC 
AAGATTACCG ATTCCAACAT CGAAGCCGCC GTCGAGCCCC TCACCCATTC CGACCATGAG 
GATGTCGCGT CGCAGTCCAA ACGATTGCTT CAGGAATGGA GCAAATTGGA GACAGCCTAT 
CGCATCCCGC GCAAGAAGCT CGACCCCTCC GCTCCCGTAA CCACAAATTC GTTTGAGGAT 
GACCGCCGCA ACGTCAATCA TGAGGAGCAT CCCTCAAGAC CTGTCAATCC GTTCGAGAAC 
ATGGTTGTGC CCACGGGTCC CCGCAGCAAC ATTCCCCAAC GGAACATGAA CTACTTCAAC 
AACCAGAGAC CACGCAAGCT TCCAACGAAT CTTCCTGCAG GATGGTTTGT CACCACTGAC 
AGCACAGGCA AATACTACTT TTACGACAAG AGCGGCCATA CCCAGTGGCA GCGACCTACA 
ACGCCTGCTG TCGACGTGCC CAAGCCCTCT GCCAAGGTGG AACAAAACCA AAAGGCTCTG 
CAGGATATCA TCGACAGTTT GACCAAGGAG CCGACGCCCC GCCATTCTGC GAACCAAACC 
CCTAAATCTA ACACACCCGT GCCAGACAAT GGTAAGAAGG AGAAGTGGAG GTCTCTTCCG 
GTTGAGAAAC AGATGAAGAT TTACGAAAAC ACGCTCTTTC CTCATGTGAA ATACGTCATG 
GACAAGTTTC ACCGAAGACT TCCCAAGGAA GACCTCAAGC GGTTTGGCCG TGAGATCAAC 
AAGAAACTGG TTGCTTCCGA CTACAAGAAC CACCGTGTCG ATGACCCTAC GACCATTTCC 
TCCAACCAGG CAAGGAAGAT CAAGAAATTT GTCAAGGACT TCTTTGATCG TGCTGTGGTC 
AAGCACAGGG AAAACGAGCA GCGCGCTGCA CAGAAAGCCG GGCCATCATC TTCCGGCGCC 
CCATCGCCTA CCAACGGCGG TTCAGCCAAG TCACCACTTG GCCGTAACGG CGCCAGCAAT 
GCCACCGTCA AGACCCAGCA GCCCGACGCC GATGGCGATA TTGTCCTTAC AGACGTGGAG 
GATGAGGGCG AGAATACTCC TGCCACCTCC AGCTCAGACC GCAAGAGGAA GCGTGCGGAA 
GAGCAAGAGG TTCCGGCGCC GGCATCCGAA GCGATACCTT CACCGAAGCG AGCCAAGGAG 
GACTCGACAA CCGAAGACTC GATCCCCAGC CCTCCGCCCC CTCCGCCGCC GCCGACAGAT 
ACTCCACTCA CCGAAGAGGA GCGATCAATG CGCGAGCAAG AGGAGGCCTT AATGAGGGAG 
AACGAAGAAG CGCAGAGGCT GGAGGACGAG GAGGCCGAGC GCCGGGTGAG TGTTACTGTT 
CAGGGAGCGG CTGTCGCGAC CAGCAAGGTG AACGGAGTAA ATGGGACCAA GGCCCACCAT 
ACCTCAGAGG CATCACCTGC TGTTTCCGAT GAGAATGGGA TGGACGCTGG TCGTGATGAG 
AAGAGTCATG AGCAAATGGC ACAACAGGAG CCCGTCAGTC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_957740.1
CDS	727..3591
Translation	MEDGHHSPDSTKESKSEEHSKMNGSKLKRDGASNAGNAATPNGSQTGISRPPSMSPDEHKAASESTATPSENVPTQKPSRKASQKNMKREPVLFNHLPDVREEACTHFQVIHDCLYGSKNMGASEHDALDCDCAEEWRGDMNHACGEDSDCINRATKMECVDGDCNCGSGCQNQRFQRKQYADVSVIKTEKKGFGLRANTDLQVNDFIFEYIGEVINEPTFRSRMVKYDKEGIKHFYFMSLTKSEFVDATKKGNLGRFCNHSCDPNCYVDKWVVGDKLRMGIFAGRAIKAGEELVFNYNVDRYGADPQPCYCGEPNCTGFIGGKTQTERATKLPPATIEALGIEDGDSWDTAVAATVKKPRKKKATEDDEEYINRFEPRGLDEEGVTKVMATLMQCKEKWIAVKLLGRLQNADDDHVRNRVVKMHGYQILKTTLNTFKEDTNVVLQILDILYQLPRITKNKITDSNIEAAVEPLTHSDHEDVASQSKRLLQEWSKLETAYRIPRKKLDPSAPVTTNSFEDDRRNVNHEEHPSRPVNPFENMVVPTGPRSNIPQRNMNYFNNQRPRKLPTNLPAGWFVTTDSTGKYYFYDKSGHTQWQRPTTPAVDVPKPSAKVEQNQKALQDIIDSLTKEPTPRHSANQTPKSNTPVPDNGKKEKWRSLPVEKQMKIYENTLFPHVKYVMDKFHRRLPKEDLKRFGREINKKLVASDYKNHRVDDPTTISSNQARKIKKFVKDFFDRAVVKHRENEQRAAQKAGPSSSGAPSPTNGGSAKSPLGRNGASNATVKTQQPDADGDIVLTDVEDEGENTPATSSSDRKRKRAEEQEVPAPASEAIPSPKRAKEDSTTEDSIPSPPPPPPPPTDTPLTEEERSMREQEEALMRENEEAQRLEDEEAERRVSVTVQGAAVATSKVNGVNGTKAHHTSEASPAVSDENGMDAGRDEKSHEQMAQQEPVSR

Target ORF information:

RefSeq Version	XM_952647.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A histone-lysine N-methyltransferase (set-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_952647.2

ORF Insert Sequence:

ATGGAGGACG GCCATCACTC ACCGGATTCG ACCAAGGAAT CAAAGTCGGA GGAACACTCC 
AAGATGAACG GCTCCAAGCT AAAACGGGAT GGCGCCAGCA ACGCCGGCAA TGCGGCCACG 
CCAAACGGCT CCCAGACCGG AATCTCGAGA CCCCCAAGCA TGTCGCCCGA CGAGCACAAG 
GCTGCGAGTG AGAGCACAGC AACTCCCAGC GAAAATGTCC CGACACAAAA GCCTTCGCGA 
AAGGCGTCTC AGAAGAACAT GAAGAGGGAA CCGGTGCTCT TCAACCATCT CCCCGACGTG 
AGGGAAGAGG CATGCACACA TTTCCAGGTC ATTCATGACT GTCTCTATGG CTCAAAGAAC 
ATGGGTGCAT CCGAGCACGA TGCGTTGGAT TGTGACTGTG CGGAAGAATG GCGCGGTGAT 
ATGAACCATG CGTGCGGGGA AGATTCAGAC TGTATTAATC GGGCCACGAA AATGGAGTGC 
GTTGACGGCG ATTGCAACTG TGGTAGCGGA TGCCAGAACC AGCGCTTTCA GCGAAAACAG 
TACGCCGACG TATCGGTCAT CAAGACGGAA AAGAAGGGGT TCGGCCTCCG GGCAAACACG 
GATTTGCAAG TTAACGACTT CATTTTCGAG TACATTGGCG AGGTCATCAA CGAGCCGACA 
TTTCGCAGCC GCATGGTCAA ATACGACAAG GAGGGCATCA AGCACTTCTA TTTCATGTCA 
CTAACCAAAT CCGAGTTTGT TGATGCCACT AAGAAGGGCA ACCTCGGCAG GTTCTGCAAC 
CATTCGTGCG ATCCTAATTG CTACGTCGAC AAGTGGGTGG TTGGTGACAA GCTGCGCATG 
GGCATTTTTG CGGGGCGCGC AATCAAGGCT GGTGAGGAAC TGGTGTTCAA CTACAACGTG 
GACCGCTATG GCGCCGACCC GCAGCCTTGC TACTGCGGTG AACCAAACTG CACCGGTTTC 
ATTGGCGGCA AAACGCAAAC AGAGCGTGCA ACCAAACTGC CGCCTGCCAC CATCGAGGCG 
CTTGGTATCG AAGATGGAGA CAGCTGGGAC ACGGCGGTGG CGGCCACCGT CAAGAAGCCC 
CGCAAGAAGA AGGCTACCGA GGACGATGAA GAGTACATCA ACCGCTTCGA GCCTCGTGGC 
CTCGATGAAG AGGGTGTCAC CAAGGTCATG GCCACGCTCA TGCAGTGCAA GGAGAAATGG 
ATTGCCGTCA AGCTGCTCGG CCGCCTTCAG AATGCCGACG ATGACCATGT CCGGAACCGG 
GTTGTTAAGA TGCACGGATA CCAGATTTTG AAGACAACCC TCAACACCTT TAAGGAAGAC 
ACCAATGTCG TACTGCAGAT TCTCGATATC CTGTACCAAC TCCCCCGCAT CACCAAGAAC 
AAGATTACCG ATTCCAACAT CGAAGCCGCC GTCGAGCCCC TCACCCATTC CGACCATGAG 
GATGTCGCGT CGCAGTCCAA ACGATTGCTT CAGGAATGGA GCAAATTGGA GACAGCCTAT 
CGCATCCCGC GCAAGAAGCT CGACCCCTCC GCTCCCGTAA CCACAAATTC GTTTGAGGAT 
GACCGCCGCA ACGTCAATCA TGAGGAGCAT CCCTCAAGAC CTGTCAATCC GTTCGAGAAC 
ATGGTTGTGC CCACGGGTCC CCGCAGCAAC ATTCCCCAAC GGAACATGAA CTACTTCAAC 
AACCAGAGAC CACGCAAGCT TCCAACGAAT CTTCCTGCAG GATGGTTTGT CACCACTGAC 
AGCACAGGCA AATACTACTT TTACGACAAG AGCGGCCATA CCCAGTGGCA GCGACCTACA 
ACGCCTGCTG TCGACGTGCC CAAGCCCTCT GCCAAGGTGG AACAAAACCA AAAGGCTCTG 
CAGGATATCA TCGACAGTTT GACCAAGGAG CCGACGCCCC GCCATTCTGC GAACCAAACC 
CCTAAATCTA ACACACCCGT GCCAGACAAT GGTAAGAAGG AGAAGTGGAG GTCTCTTCCG 
GTTGAGAAAC AGATGAAGAT TTACGAAAAC ACGCTCTTTC CTCATGTGAA ATACGTCATG 
GACAAGTTTC ACCGAAGACT TCCCAAGGAA GACCTCAAGC GGTTTGGCCG TGAGATCAAC 
AAGAAACTGG TTGCTTCCGA CTACAAGAAC CACCGTGTCG ATGACCCTAC GACCATTTCC 
TCCAACCAGG CAAGGAAGAT CAAGAAATTT GTCAAGGACT TCTTTGATCG TGCTGTGGTC 
AAGCACAGGG AAAACGAGCA GCGCGCTGCA CAGAAAGCCG GGCCATCATC TTCCGGCGCC 
CCATCGCCTA CCAACGGCGG TTCAGCCAAG TCACCACTTG GCCGTAACGG CGCCAGCAAT 
GCCACCGTCA AGACCCAGCA GCCCGACGCC GATGGCGATA TTGTCCTTAC AGACGTGGAG 
GATGAGGGCG AGAATACTCC TGCCACCTCC AGCTCAGACC GCAAGAGGAA GCGTGCGGAA 
GAGCAAGAGG TTCCGGCGCC GGCATCCGAA GCGATACCTT CACCGAAGCG AGCCAAGGAG 
GACTCGACAA CCGAAGACTC GATCCCCAGC CCTCCGCCCC CTCCGCCGCC GCCGACAGAT 
ACTCCACTCA CCGAAGAGGA GCGATCAATG CGCGAGCAAG AGGAGGCCTT AATGAGGGAG 
AACGAAGAAG CGCAGAGGCT GGAGGACGAG GAGGCCGAGC GCCGGGTGAG TGTTACTGTT 
CAGGGAGCGG CTGTCGCGAC CAGCAAGGTG AACGGAGTAA ATGGGACCAA GGCCCACCAT 
ACCTCAGAGG CATCACCTGC TGTTTCCGAT GAGAATGGGA TGGACGCTGG TCGTGATGAG 
AAGAGTCATG AGCAAATGGC ACAACAGGAG CCCGTCAGTC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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set-2 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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