NCU00050 cDNA ORF clone, Neurospora crassa OR74A

The following NCU00050 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00050 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf05254 XM_951068.3
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Neurospora crassa OR74A pyruvate dehydrogenase X component (NCU00050), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf05254
    Clone ID Related Accession (Same CDS sequence) XM_951068.3
    Accession Version XM_951068.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product pyruvate dehydrogenase X component
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_951068.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCTTCGC TAACAGCAGC TTGTCGCATC TCTGCCAGAA TGGCAGGGCG CTCCGTCAGA 
    GGTTTCCGTA CCTCTGCCGC CGCTCTCGCC GCCCAAAACT TTACCATGCC CGCTCTGTCG
    CCGACAATGA CGGAGGGCAA TATCGCGACC TGGAGGGTGA AGGAGGGCGA CAAGTTCTCT
    GCCGGTGACG TGTTGTTGGA AATCGAGACC GACAAGGCCA CTATGGACGT TGAGGCGCAG
    GATGACGGTG TCATGGTCAA GATCATGAAG AACGATGGCG CCAAGGGTGT TGCTGTCGGC
    GCCCGGATAG CTGTTATTGC GGAGGAAGGA GATGACATTA GCTCTCTCGA GATCCCAGCC
    GACGCAGCCC CACAGTCCAA GCCAGCCGAG TCAGCACCCT CGGCACCTCC CCCACCGACC
    ACCGCGGACC AGTCTAACGT TGCCGTCCCC GAATCCGCTC CTCAGAATGC TTCGTCCAAG
    TCGGCTCCCA AGCCTCCGAA GAGGCAATAC CCCCACTATC CTTCCGTCGC CCATCTCCTC
    AAGGTCAACG GCATTGACGC CGCTGCCGTT AAGGATATCA CCCCAACCGG CCCCGGCGGC
    CGTCTGCTCA AGGGTGACGT TCTTGCTTAC CTTGGTAAGA TTAACGCCCA GACGCCGTCC
    ACCGTCTCCG AGAGGTTCGA GAAGCAGTCG CACCTCGACC TTAGCAACAT CAAGGTTGCC
    AAGAGCACCG AGGCTGTCAA GGCCACTACC GAGAAGGCTC AGTCCAAGAA GCTGGATGCT
    CCCGCTCCTC CTCCCGTAGC TGTTGTCACC GCGCCTATTT CTCTGTCCGC CGCCATCGAT
    GTTCAGAACA AGCTTCACAA AACCATCGGC GTCTTCCTCC CTCTTTCAAC CTTCATTACC
    CGTGCCACCG AGATCGCCAA CCAGAAGCTA CCCCTTCCCG CAAACTACCA GCCTACTGCC
    GATGAGCTCT TCAACCAGGT TCTCGGTCTT GACAAGGTCA CTCGCAAGGA ATCTCGCGGC
    TCCTATACTC CCACGTTCGG CTCCTTCGTG GCCCCTCAGC GGGCAGCTCG CAAGGCTGAT
    ATCATCGATA TTCTCGCCGC TCCCTCCACG CGCGTTGCTG CTTCTGCTCA GAGCAAGTCC
    GCCGCCCCCG GATTGACCAC TTCTGGACCC AACGTCTTCA GCCTTCAGGT ACCCAAGTCG
    GAAGAGAAGA GGGCCCAGGC CTTCCTTCAG AAGATGAAGC TTGTTTTGGA ACAGGAGCCT
    GACAAGCTTG TACGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_956161.2
    CDS345..1625
    Translation

    Target ORF information:

    RefSeq Version XM_951068.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A pyruvate dehydrogenase X component (NCU00050), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_951068.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCTTCGC TAACAGCAGC TTGTCGCATC TCTGCCAGAA TGGCAGGGCG CTCCGTCAGA 
    GGTTTCCGTA CCTCTGCCGC CGCTCTCGCC GCCCAAAACT TTACCATGCC CGCTCTGTCG
    CCGACAATGA CGGAGGGCAA TATCGCGACC TGGAGGGTGA AGGAGGGCGA CAAGTTCTCT
    GCCGGTGACG TGTTGTTGGA AATCGAGACC GACAAGGCCA CTATGGACGT TGAGGCGCAG
    GATGACGGTG TCATGGTCAA GATCATGAAG AACGATGGCG CCAAGGGTGT TGCTGTCGGC
    GCCCGGATAG CTGTTATTGC GGAGGAAGGA GATGACATTA GCTCTCTCGA GATCCCAGCC
    GACGCAGCCC CACAGTCCAA GCCAGCCGAG TCAGCACCCT CGGCACCTCC CCCACCGACC
    ACCGCGGACC AGTCTAACGT TGCCGTCCCC GAATCCGCTC CTCAGAATGC TTCGTCCAAG
    TCGGCTCCCA AGCCTCCGAA GAGGCAATAC CCCCACTATC CTTCCGTCGC CCATCTCCTC
    AAGGTCAACG GCATTGACGC CGCTGCCGTT AAGGATATCA CCCCAACCGG CCCCGGCGGC
    CGTCTGCTCA AGGGTGACGT TCTTGCTTAC CTTGGTAAGA TTAACGCCCA GACGCCGTCC
    ACCGTCTCCG AGAGGTTCGA GAAGCAGTCG CACCTCGACC TTAGCAACAT CAAGGTTGCC
    AAGAGCACCG AGGCTGTCAA GGCCACTACC GAGAAGGCTC AGTCCAAGAA GCTGGATGCT
    CCCGCTCCTC CTCCCGTAGC TGTTGTCACC GCGCCTATTT CTCTGTCCGC CGCCATCGAT
    GTTCAGAACA AGCTTCACAA AACCATCGGC GTCTTCCTCC CTCTTTCAAC CTTCATTACC
    CGTGCCACCG AGATCGCCAA CCAGAAGCTA CCCCTTCCCG CAAACTACCA GCCTACTGCC
    GATGAGCTCT TCAACCAGGT TCTCGGTCTT GACAAGGTCA CTCGCAAGGA ATCTCGCGGC
    TCCTATACTC CCACGTTCGG CTCCTTCGTG GCCCCTCAGC GGGCAGCTCG CAAGGCTGAT
    ATCATCGATA TTCTCGCCGC TCCCTCCACG CGCGTTGCTG CTTCTGCTCA GAGCAAGTCC
    GCCGCCCCCG GATTGACCAC TTCTGGACCC AACGTCTTCA GCCTTCAGGT ACCCAAGTCG
    GAAGAGAAGA GGGCCCAGGC CTTCCTTCAG AAGATGAAGC TTGTTTTGGA ACAGGAGCCT
    GACAAGCTTG TACGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B