NCU02052 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU02052
Entrez Gene ID	3880157
Full Name	transcription initiation factor TFIId 127kD subunit
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011394625.1	XP_011392927.1	transcription initiation factor TFIId 127kD subunit
XM_011394624.1	XP_011392926.1	transcription initiation factor TFIId 127kD subunit, variant

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR674C	NP_986222.1
Magnaporthe oryzae (rice blast fungus)	MGG_03637	XP_003716252.1
Saccharomyces cerevisiae (baker's yeast)	TAF2	NP_009971.1
Schizosaccharomyces pombe (fission yeast)	taf2	NP_593331.2
Neurospora crassa	NCU02052	XP_964008.1
Kluyveromyces lactis	KLLA0C02189g	XP_452294.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU02052 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02052 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf02469	XM_011394625.1 Latest version!	Neurospora crassa OR74A transcription initiation factor TFIId 127kD subunit (NCU02052), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ONf02469	XM_011394624.1 Latest version!	Neurospora crassa OR74A transcription initiation factor TFIId 127kD subunit, variant (NCU02052), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU02052 ( XM_011394625.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011392927.1 Neurospora crassa OR74A transcription initiation factor TFIId 127kD subunit (NCU02052), mRNA.

Price & Availability↑

CloneID	ONf02469
Clone ID Related Accession (Same CDS sequence)	XM_011394625.1 , XM_011394624.1
Accession Version	XM_011394625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	transcription initiation factor TFIId 127kD subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCAGGAC TCGAGAATGG AGATGTGGCC CTTGCCAACA TGACGAACGC CCCCGATCCT 
GTGGCCGCTG CCGAGCCGTA CGCCATTTTG AGGCAAGAGG TGGAGCTAGA CATCAACTTC 
AGGGAGAAGA GCGTCAGCGG CATTTGCACA TTCAAGATTT ATCCCCTGGT TCCCGACCTT 
GAGGAACTCG TCCTAGACAC GCGGCAATGC GAAATCGACG TTTCAAACAT TACGGTGGAC 
GGCTACAAGA CACGGGCACA GGCTCATGAT CCTTACGATC GAATGGAGCC CCCCACGAAT 
GCGCAAATCG GTGCCACTCA GCATCACATA ATGAAGAAAT GGATGGCGCC ATTACTCCCA 
GAAACACGCC GCCAGGCTCC CATCGTAGAA AGGGCAGAGC AGCTTAATAT CAGCGTGCCG 
GCTGATGGGT CCCTCAGGAT CCGTCTCCGC CCAGATGCTC TCATCCAGGA TTCGGAGCCC 
AAACGCATTC TCAAGATCAA AACTCCCAAG TCGGTATCGG AAAATGCGAC AGGCCTCTCC 
GACCCTAATG AGGAACTCAC GGTGGTTGTG CCTTTCAGGT CCAAGAGAAT CAGAGATGGC 
CTGCACTTTG TCGGCGTTGA GGAAGGTGAC CTGAGATACC CACACGTTTA CACCCGGCAT 
TCGCTCGAGC CCGGTACGGC GTCTTGCATC TTCCCTTGCC TCGATGATCC CGGTTCGAGG 
CATTCCTGGA CAATCAAAAT CACATGCCCA AGAACGCTGG AGGATGCCTT CGCACCGCCT 
CTGGCTACCC AATCAGCACT GCTGACTGCT CTGGATGGCG GTCGGAAACG CAAAGCCTCT 
GATATTTCTG CACACCAGCC TGGCTCATCT TTCACCGAAG AGGATAAGCT GAGGGAGATT 
ACCGTGGTCT GCTCTGGCAA TCTCATTGGC GAGCAGATGC ACCCCAAGGA CGAGCGCAAG 
AAGACCATGA TTTTTGAGTG CGTCAACACC GCTGCTAGGC ACATCGGTTT CGCCGTTGGC 
CCCTTTGAGC ACGTTGATCT TGGGGCCGAG TTTAGAACTG AGGAGGCGGA CGAGAAGCTC 
GGGGCTAATG CAGCCAGAAT TCACGGCTAT TGTCTCCCTG GCCGTGTGGA TGAAGTTCGA 
TGGACATGCC AAGCCGTCGT GGCTGCTGCC GACTACTTCG CCCCTGAGTT CGGCCGTTAT 
CCTTATGAAG CCTACAAGAT GTGCTTTGTC GAAGACTTGG TTCACGACAC TGCGGTTGCA 
AACTCCTTAT CCTTGTGCAG TACGCGTTTG CTGTACCCCG AAACGATCAT AGATACGGAC 
ATCGAGGTTA CCAGGAAGCT CGTCCATGCC CTCGCCAGTC AGTACGTGGG GGTCCACATT 
GTGCCAAATG AGCGGTCCGA CACTTGGCTT ATCATCGGTC TCCAGTGGTT CATGACAGAT 
CTGTTCATGA GGACCATCTG CGGAAACAAC TGGTATCGTT TTCATATCAA GTCGCTTTCC 
GACAAGCTTG TAGAGGCGGA TGTGCGGAGA CCCTCCTTGC ATGCTCTGGG CGATTATCTT 
CACCTAGGTG ACTTTGAGCT CGAGTTTATG GCGCTCAAGG CGCCACTTGT CCTTTACATA 
CTCGATCAGC GCATGTCCAA GATTCCTGGC TCGACAGGTG TCGTCCGCGT TTTGTCGCAA 
ATGGTTTCGG CCGCTAATAT TTCCAACACG GACCCCAAGG CCACTACCCT CTCTTTCCAG 
GACTTCCGTC GATGGTGCGA GAAACGCAGT CAATACGTCC CTGATGAGCT TTGGAATCAA 
TACGTGTTTG GTGCGGGTTG CCCGAGACTG GATATTCACC AAAAGTTCAA CAAGAAGAAT 
CTCAACGTCG ACATCCAAAT TCTACAATAT CAGGGACCGA CCAAGAAAAG CATATCAAAG 
GATGAGTTCT GGAGGGAGTT GCAAGAGGAG ATACACCAGG TCTATGCCGG CGACGTTCCC 
AAGCTGTTCA CTGGTCCATT CACCGTCCGA ATTCACGAAG CAGACGGTAC GCCTTATGAG 
CACTACCAGC TCATTACCGA GAAAGATAAA GGTGGGACGA ATTTGCAGAT TGCGTACAAT 
ACAAAGTACA AGCGTCTGAA AAGAACCAAA AAGGCCATGG CCGCGGCTGC CAACAGCAAT 
AGTGACAAAC ACGAGATCCA GGAGGACGAT GTTGTCTACT TTAACATGTT GGGCGATGTC 
CTCACCAGCC AAAAGGATAT GGAGGACTGG GGGTTACAAG ACTGGGCTTC AGAGGTCCAA 
ACCAAGATGG ATCAAGAATC CTATGAATGG ATCCGGCTTG ATGCTAACAT GGAGTGGCTT 
TGCTTCATGA AGACTGATAT GCAGGAGTAC ATGTATCTGG CACAACTACA GCAGGATAGA 
GACGTCGTGG CCCACCAGGA CGCGATGCTC GCTTTCAAGA GGGAAAAGAG GCATGCGGTC 
CACTCGACGA TCGAGACGCG GACAGTGATG GACCGACGAT ATTACCATGG TGTCCGAGTG 
ATGGCATTGG AAGACCTCGC TAAACAGGCA CATCCTGACT TGAATTACAT CGGCATGGTC 
CATCTGATAC TCTGTTTCCG CAAGTTTTTC TGCAAGAAGG TACCAATTCC CGGCCGGGAG 
GGTGGCTATC ACTACCCGAT CGCTCCCAAC GATTTTACCG ACAAGGGCCA GTATGCCATC 
CAATGTGCGA TTCCCGCGGT GCTTGCACGG ACGACGGACC TGCAGGAAAA AGGAAGATGC 
TCTAAGAGGG CCCGACAGTT CATCATGGAG ATGCTCCTGT TTAATGACAA TTCGGAGAAT 
GAGTTTTCAG ACCAGTTCTA CATCGCCAAA CTCCTCGATG CTCTTACCAC CTCTGTGATT 
CCGCAGAAGG AGATTACCGA GGGATACCTC ATCAAAAACC TGGATCCTTT CGATGAAGAC 
GATGCGGAAT TCAAAAGTTT CATTGAGAAG ACCATTGAGG AGATCGACAA GTTTCGCAGG 
ATGGATGAAT GGACACTGAC ATACCAGAAC ATTTGGACGA CAACGGCTCT TCACTGCAAG 
ATGAGGCTCA TGAAGGCTCG TGTCATCCCT GAATCGGCCC TCGACTTTGT TCAGTACCTC 
CAGGACGACA ACATTGATCT TGTACGTATC AAGGCGTTTG AGTGCCTGGT TGAGCTTGGA 
ATGATCTCCA AACCCCCTAT TCTCAAGCTT CTACTTTCAT ACATGAGCAC CGATAGTTCG 
CCCTTCATCC GCGATCGATT GTTCAAGGTC TTTTGCCGGG GAATCGCTGC CATTGCGTTT 
GGCGAGAACA AAGCTTCGAC ACAGGAACTT GAAGCTCTTA ATGATGGAGG TCTCGTCATC 
GATCAAGGTG ACGCAGAGAT CCAACAACGG AAGCTCGATG CAGTACGCAG AGAAGACATT 
GCCCACGCGC TTAATGCGCT CAAGGAAGAG CTGAGGGGGA ACACGGAGAT GCAAATATCC 
ATATGGAAGG CATTTAATTC TCCCGTCATC GGTCTACAGG AGAAGCGCCA GCTATTGGAG 
CTCTGCTCGG CCATGTTCGA ACCAGAAGAT TCTCTACTTG TTACTATCAA GTATCCCATT 
GTCTGGAAGG CAACTCGCGA CCCGACAATT GTCGCCACTG CACCACAGAT CCTCGGAACT 
CCGCCCAAGA AGAGATGTGT CGTCAAGTTC AAGTCGGAGT ACCGGACGCT TCCTAAGAGT 
AAGGATATGG TTGCGATCGA AGCTCCACCG CCTCCGGCGC CATTACCCGC ACCGACACCG 
TCGACCAACT TGTCGGCTTC CAGGGTAGAG ATTAAGCCTC CTGAGAAGAA GACAATCGTA 
AAGCTACAAT CACGGCCTTC GTACATTGCG AAACCTCGGG AGTCACCAGT CCCAGAAGCT 
CCCCCACCAG TACGGAAGGA CTCTATCGCT ATGACCGTGC CTCCTCGGCC TTCTACGACT 
GCCGGTCCGC GCACTCCCTT GCCCACATCA GTTCCAAGTG CTCCCGCACC CAATACATCC 
GCCGCTCAGC CTAGCATCAA GACAGCTGTA CCAAAAGCGG CTTCACCTCC CCCCGTACCT 
GCACCCAAAC CCCAACAGTC CAACGACGTT GCCGTTCCAA GGCCTAGCAC CGTCAATTTG 
GGCAAGCCAA AGATCGTCAA GCCGGTCATC AAGCGCAAGA GTGACGAGTC ATTTGAGAAC 
CCTAGCCCGA AGAAGATCAT CAAGACCAAC AGCGACTCCT TCCGGAGCGA TCCTTCTCGC 
AATAGCTCAT TCAAGACAGA ACCCCCTCGC AGTGAGTCCT TTAAGAAAGA GGCTCCTCGA 
AGCGAAGCCA TCAGGGGCCA CTCTTTCCGC GAATCCGCCC CTCAGATTCC GCCTCCCCGC 
ACATCTTCAT TCTCCGTCCG TCCCAACGCC AATGGCCAGC CTTCCAAAGT CGTCACGCTA 
CCATTTAGGA AATGGACCAA GCTGTCTGCC CGCGCCCAGC GCAGCATCGA CTACGAACAA 
AGTCAGCGTG AAAAGCAAAT GCTTGCCGCC AGCGCCAGAG GCATTTCCGG CAGTCTTGCC 
ACACCCGTCC ACAAGCGATC GAGCGCCGCA GTAGGATTTG GCAGCGGTTC GGCGCCTTCA 
TCCCCTAAGA GCCGGATGCA GTCTCCTGCC GGTAACGGAT ACGGCTCGTC TGGATCGTAT 
GCTGAGAAGG AGCAGCGGAA TAAGGAGAGG GATAGAGACA GGGAACGGGA CAGAGATAGG 
GAGAGAGACA GCAGTAGCAA ATTCCGGGAG AAGGACCGCA ATGATCACGA CAGGGAGCGA 
TACCGTGGTG GTGCCGAGCG AGAAAAGGAG AAGTACAGGG AGCGGGAGCG GGACCGCGAG 
AGTGACCGTG AGCATGAGCG TCCTCGCGAG AAAGAGCGGG ATAGAGAGAA GTACAGGGAA 
CGAGGAGACC GCGATAGTGA TCGTGAGCGC GACCGACCTC GTGAGAAGTA CAGGGAGAAG 
GGAACCGACC GCGGCGATAG CGACAGGGGC GGCCGCACCA CCGACAGCGA CCGGGAACGG 
AACCGGATCG ATCGGGATCG AGATCGGGAT CGGGAACGGG AACGCGAGAG GAGTGACCGT 
GACCGCGAGA GAACTGACCG CGAGAGGGAG CGGATTCGCG ACAAGTACCG TGATCGAGCT 
GATCGGGACA GCGATCGTGA TAGAGACAGT TACAAGGATC GTGATCGGGA GCGCGACCGC 
GACCGTGATA GAGACAGGGA GCGTGATCGT GGTGATCGTG GTGATCGAGG TGACCGAGAC 
CGGGACCGGG ACCGAGACCG AGACCGAGGA GATCGCGACA GGGTATCATC CTCCGGCTCA 
AGTCTCCTTA TCAAGAAGGA CAGAAAACCG TTGCCAGGAT CCGGAGAAAA AGAGCGGAGA 
CCATTGCCTA CTGGAGCGCC TCACGAAAAG GCACCGCATC CCCTCAAGGA ATCCCATTCC 
CCAATCCATC CGACTCCTGC TGCCACAACC AACGGCGGCG AGCCAAAGAA GAAAATCATT 
AAGCTCAAGT TGAAGAGTAG CGGAAGTGGT CTCGGAGGTG GTTCTGGTTC GTCATCGACG 
CCTGGTGCTG GTGGTGGTAG TGGATCTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011392927.1
CDS	1011..6623
Translation	MSGLENGDVALANMTNAPDPVAAAEPYAILRQEVELDINFREKSVSGICTFKIYPLVPDLEELVLDTRQCEIDVSNITVDGYKTRAQAHDPYDRMEPPTNAQIGATQHHIMKKWMAPLLPETRRQAPIVERAEQLNISVPADGSLRIRLRPDALIQDSEPKRILKIKTPKSVSENATGLSDPNEELTVVVPFRSKRIRDGLHFVGVEEGDLRYPHVYTRHSLEPGTASCIFPCLDDPGSRHSWTIKITCPRTLEDAFAPPLATQSALLTALDGGRKRKASDISAHQPGSSFTEEDKLREITVVCSGNLIGEQMHPKDERKKTMIFECVNTAARHIGFAVGPFEHVDLGAEFRTEEADEKLGANAARIHGYCLPGRVDEVRWTCQAVVAAADYFAPEFGRYPYEAYKMCFVEDLVHDTAVANSLSLCSTRLLYPETIIDTDIEVTRKLVHALASQYVGVHIVPNERSDTWLIIGLQWFMTDLFMRTICGNNWYRFHIKSLSDKLVEADVRRPSLHALGDYLHLGDFELEFMALKAPLVLYILDQRMSKIPGSTGVVRVLSQMVSAANISNTDPKATTLSFQDFRRWCEKRSQYVPDELWNQYVFGAGCPRLDIHQKFNKKNLNVDIQILQYQGPTKKSISKDEFWRELQEEIHQVYAGDVPKLFTGPFTVRIHEADGTPYEHYQLITEKDKGGTNLQIAYNTKYKRLKRTKKAMAAAANSNSDKHEIQEDDVVYFNMLGDVLTSQKDMEDWGLQDWASEVQTKMDQESYEWIRLDANMEWLCFMKTDMQEYMYLAQLQQDRDVVAHQDAMLAFKREKRHAVHSTIETRTVMDRRYYHGVRVMALEDLAKQAHPDLNYIGMVHLILCFRKFFCKKVPIPGREGGYHYPIAPNDFTDKGQYAIQCAIPAVLARTTDLQEKGRCSKRARQFIMEMLLFNDNSENEFSDQFYIAKLLDALTTSVIPQKEITEGYLIKNLDPFDEDDAEFKSFIEKTIEEIDKFRRMDEWTLTYQNIWTTTALHCKMRLMKARVIPESALDFVQYLQDDNIDLVRIKAFECLVELGMISKPPILKLLLSYMSTDSSPFIRDRLFKVFCRGIAAIAFGENKASTQELEALNDGGLVIDQGDAEIQQRKLDAVRREDIAHALNALKEELRGNTEMQISIWKAFNSPVIGLQEKRQLLELCSAMFEPEDSLLVTIKYPIVWKATRDPTIVATAPQILGTPPKKRCVVKFKSEYRTLPKSKDMVAIEAPPPPAPLPAPTPSTNLSASRVEIKPPEKKTIVKLQSRPSYIAKPRESPVPEAPPPVRKDSIAMTVPPRPSTTAGPRTPLPTSVPSAPAPNTSAAQPSIKTAVPKAASPPPVPAPKPQQSNDVAVPRPSTVNLGKPKIVKPVIKRKSDESFENPSPKKIIKTNSDSFRSDPSRNSSFKTEPPRSESFKKEAPRSEAIRGHSFRESAPQIPPPRTSSFSVRPNANGQPSKVVTLPFRKWTKLSARAQRSIDYEQSQREKQMLAASARGISGSLATPVHKRSSAAVGFGSGSAPSSPKSRMQSPAGNGYGSSGSYAEKEQRNKERDRDRERDRDRERDSSSKFREKDRNDHDRERYRGGAEREKEKYRERERDRESDREHERPREKERDREKYRERGDRDSDRERDRPREKYREKGTDRGDSDRGGRTTDSDRERNRIDRDRDRDRERERERSDRDRERTDRERERIRDKYRDRADRDSDRDRDSYKDRDRERDRDRDRDRERDRGDRGDRGDRDRDRDRDRDRGDRDRVSSSGSSLLIKKDRKPLPGSGEKERRPLPTGAPHEKAPHPLKESHSPIHPTPAATTNGGEPKKKIIKLKLKSSGSGLGGGSGSSSTPGAGGGSGSR

Target ORF information:

RefSeq Version	XM_011394625.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A transcription initiation factor TFIId 127kD subunit (NCU02052), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394625.1

ORF Insert Sequence:

ATGTCAGGAC TCGAGAATGG AGATGTGGCC CTTGCCAACA TGACGAACGC CCCCGATCCT 
GTGGCCGCTG CCGAGCCGTA CGCCATTTTG AGGCAAGAGG TGGAGCTAGA CATCAACTTC 
AGGGAGAAGA GCGTCAGCGG CATTTGCACA TTCAAGATTT ATCCCCTGGT TCCCGACCTT 
GAGGAACTCG TCCTAGACAC GCGGCAATGC GAAATCGACG TTTCAAACAT TACGGTGGAC 
GGCTACAAGA CACGGGCACA GGCTCATGAT CCTTACGATC GAATGGAGCC CCCCACGAAT 
GCGCAAATCG GTGCCACTCA GCATCACATA ATGAAGAAAT GGATGGCGCC ATTACTCCCA 
GAAACACGCC GCCAGGCTCC CATCGTAGAA AGGGCAGAGC AGCTTAATAT CAGCGTGCCG 
GCTGATGGGT CCCTCAGGAT CCGTCTCCGC CCAGATGCTC TCATCCAGGA TTCGGAGCCC 
AAACGCATTC TCAAGATCAA AACTCCCAAG TCGGTATCGG AAAATGCGAC AGGCCTCTCC 
GACCCTAATG AGGAACTCAC GGTGGTTGTG CCTTTCAGGT CCAAGAGAAT CAGAGATGGC 
CTGCACTTTG TCGGCGTTGA GGAAGGTGAC CTGAGATACC CACACGTTTA CACCCGGCAT 
TCGCTCGAGC CCGGTACGGC GTCTTGCATC TTCCCTTGCC TCGATGATCC CGGTTCGAGG 
CATTCCTGGA CAATCAAAAT CACATGCCCA AGAACGCTGG AGGATGCCTT CGCACCGCCT 
CTGGCTACCC AATCAGCACT GCTGACTGCT CTGGATGGCG GTCGGAAACG CAAAGCCTCT 
GATATTTCTG CACACCAGCC TGGCTCATCT TTCACCGAAG AGGATAAGCT GAGGGAGATT 
ACCGTGGTCT GCTCTGGCAA TCTCATTGGC GAGCAGATGC ACCCCAAGGA CGAGCGCAAG 
AAGACCATGA TTTTTGAGTG CGTCAACACC GCTGCTAGGC ACATCGGTTT CGCCGTTGGC 
CCCTTTGAGC ACGTTGATCT TGGGGCCGAG TTTAGAACTG AGGAGGCGGA CGAGAAGCTC 
GGGGCTAATG CAGCCAGAAT TCACGGCTAT TGTCTCCCTG GCCGTGTGGA TGAAGTTCGA 
TGGACATGCC AAGCCGTCGT GGCTGCTGCC GACTACTTCG CCCCTGAGTT CGGCCGTTAT 
CCTTATGAAG CCTACAAGAT GTGCTTTGTC GAAGACTTGG TTCACGACAC TGCGGTTGCA 
AACTCCTTAT CCTTGTGCAG TACGCGTTTG CTGTACCCCG AAACGATCAT AGATACGGAC 
ATCGAGGTTA CCAGGAAGCT CGTCCATGCC CTCGCCAGTC AGTACGTGGG GGTCCACATT 
GTGCCAAATG AGCGGTCCGA CACTTGGCTT ATCATCGGTC TCCAGTGGTT CATGACAGAT 
CTGTTCATGA GGACCATCTG CGGAAACAAC TGGTATCGTT TTCATATCAA GTCGCTTTCC 
GACAAGCTTG TAGAGGCGGA TGTGCGGAGA CCCTCCTTGC ATGCTCTGGG CGATTATCTT 
CACCTAGGTG ACTTTGAGCT CGAGTTTATG GCGCTCAAGG CGCCACTTGT CCTTTACATA 
CTCGATCAGC GCATGTCCAA GATTCCTGGC TCGACAGGTG TCGTCCGCGT TTTGTCGCAA 
ATGGTTTCGG CCGCTAATAT TTCCAACACG GACCCCAAGG CCACTACCCT CTCTTTCCAG 
GACTTCCGTC GATGGTGCGA GAAACGCAGT CAATACGTCC CTGATGAGCT TTGGAATCAA 
TACGTGTTTG GTGCGGGTTG CCCGAGACTG GATATTCACC AAAAGTTCAA CAAGAAGAAT 
CTCAACGTCG ACATCCAAAT TCTACAATAT CAGGGACCGA CCAAGAAAAG CATATCAAAG 
GATGAGTTCT GGAGGGAGTT GCAAGAGGAG ATACACCAGG TCTATGCCGG CGACGTTCCC 
AAGCTGTTCA CTGGTCCATT CACCGTCCGA ATTCACGAAG CAGACGGTAC GCCTTATGAG 
CACTACCAGC TCATTACCGA GAAAGATAAA GGTGGGACGA ATTTGCAGAT TGCGTACAAT 
ACAAAGTACA AGCGTCTGAA AAGAACCAAA AAGGCCATGG CCGCGGCTGC CAACAGCAAT 
AGTGACAAAC ACGAGATCCA GGAGGACGAT GTTGTCTACT TTAACATGTT GGGCGATGTC 
CTCACCAGCC AAAAGGATAT GGAGGACTGG GGGTTACAAG ACTGGGCTTC AGAGGTCCAA 
ACCAAGATGG ATCAAGAATC CTATGAATGG ATCCGGCTTG ATGCTAACAT GGAGTGGCTT 
TGCTTCATGA AGACTGATAT GCAGGAGTAC ATGTATCTGG CACAACTACA GCAGGATAGA 
GACGTCGTGG CCCACCAGGA CGCGATGCTC GCTTTCAAGA GGGAAAAGAG GCATGCGGTC 
CACTCGACGA TCGAGACGCG GACAGTGATG GACCGACGAT ATTACCATGG TGTCCGAGTG 
ATGGCATTGG AAGACCTCGC TAAACAGGCA CATCCTGACT TGAATTACAT CGGCATGGTC 
CATCTGATAC TCTGTTTCCG CAAGTTTTTC TGCAAGAAGG TACCAATTCC CGGCCGGGAG 
GGTGGCTATC ACTACCCGAT CGCTCCCAAC GATTTTACCG ACAAGGGCCA GTATGCCATC 
CAATGTGCGA TTCCCGCGGT GCTTGCACGG ACGACGGACC TGCAGGAAAA AGGAAGATGC 
TCTAAGAGGG CCCGACAGTT CATCATGGAG ATGCTCCTGT TTAATGACAA TTCGGAGAAT 
GAGTTTTCAG ACCAGTTCTA CATCGCCAAA CTCCTCGATG CTCTTACCAC CTCTGTGATT 
CCGCAGAAGG AGATTACCGA GGGATACCTC ATCAAAAACC TGGATCCTTT CGATGAAGAC 
GATGCGGAAT TCAAAAGTTT CATTGAGAAG ACCATTGAGG AGATCGACAA GTTTCGCAGG 
ATGGATGAAT GGACACTGAC ATACCAGAAC ATTTGGACGA CAACGGCTCT TCACTGCAAG 
ATGAGGCTCA TGAAGGCTCG TGTCATCCCT GAATCGGCCC TCGACTTTGT TCAGTACCTC 
CAGGACGACA ACATTGATCT TGTACGTATC AAGGCGTTTG AGTGCCTGGT TGAGCTTGGA 
ATGATCTCCA AACCCCCTAT TCTCAAGCTT CTACTTTCAT ACATGAGCAC CGATAGTTCG 
CCCTTCATCC GCGATCGATT GTTCAAGGTC TTTTGCCGGG GAATCGCTGC CATTGCGTTT 
GGCGAGAACA AAGCTTCGAC ACAGGAACTT GAAGCTCTTA ATGATGGAGG TCTCGTCATC 
GATCAAGGTG ACGCAGAGAT CCAACAACGG AAGCTCGATG CAGTACGCAG AGAAGACATT 
GCCCACGCGC TTAATGCGCT CAAGGAAGAG CTGAGGGGGA ACACGGAGAT GCAAATATCC 
ATATGGAAGG CATTTAATTC TCCCGTCATC GGTCTACAGG AGAAGCGCCA GCTATTGGAG 
CTCTGCTCGG CCATGTTCGA ACCAGAAGAT TCTCTACTTG TTACTATCAA GTATCCCATT 
GTCTGGAAGG CAACTCGCGA CCCGACAATT GTCGCCACTG CACCACAGAT CCTCGGAACT 
CCGCCCAAGA AGAGATGTGT CGTCAAGTTC AAGTCGGAGT ACCGGACGCT TCCTAAGAGT 
AAGGATATGG TTGCGATCGA AGCTCCACCG CCTCCGGCGC CATTACCCGC ACCGACACCG 
TCGACCAACT TGTCGGCTTC CAGGGTAGAG ATTAAGCCTC CTGAGAAGAA GACAATCGTA 
AAGCTACAAT CACGGCCTTC GTACATTGCG AAACCTCGGG AGTCACCAGT CCCAGAAGCT 
CCCCCACCAG TACGGAAGGA CTCTATCGCT ATGACCGTGC CTCCTCGGCC TTCTACGACT 
GCCGGTCCGC GCACTCCCTT GCCCACATCA GTTCCAAGTG CTCCCGCACC CAATACATCC 
GCCGCTCAGC CTAGCATCAA GACAGCTGTA CCAAAAGCGG CTTCACCTCC CCCCGTACCT 
GCACCCAAAC CCCAACAGTC CAACGACGTT GCCGTTCCAA GGCCTAGCAC CGTCAATTTG 
GGCAAGCCAA AGATCGTCAA GCCGGTCATC AAGCGCAAGA GTGACGAGTC ATTTGAGAAC 
CCTAGCCCGA AGAAGATCAT CAAGACCAAC AGCGACTCCT TCCGGAGCGA TCCTTCTCGC 
AATAGCTCAT TCAAGACAGA ACCCCCTCGC AGTGAGTCCT TTAAGAAAGA GGCTCCTCGA 
AGCGAAGCCA TCAGGGGCCA CTCTTTCCGC GAATCCGCCC CTCAGATTCC GCCTCCCCGC 
ACATCTTCAT TCTCCGTCCG TCCCAACGCC AATGGCCAGC CTTCCAAAGT CGTCACGCTA 
CCATTTAGGA AATGGACCAA GCTGTCTGCC CGCGCCCAGC GCAGCATCGA CTACGAACAA 
AGTCAGCGTG AAAAGCAAAT GCTTGCCGCC AGCGCCAGAG GCATTTCCGG CAGTCTTGCC 
ACACCCGTCC ACAAGCGATC GAGCGCCGCA GTAGGATTTG GCAGCGGTTC GGCGCCTTCA 
TCCCCTAAGA GCCGGATGCA GTCTCCTGCC GGTAACGGAT ACGGCTCGTC TGGATCGTAT 
GCTGAGAAGG AGCAGCGGAA TAAGGAGAGG GATAGAGACA GGGAACGGGA CAGAGATAGG 
GAGAGAGACA GCAGTAGCAA ATTCCGGGAG AAGGACCGCA ATGATCACGA CAGGGAGCGA 
TACCGTGGTG GTGCCGAGCG AGAAAAGGAG AAGTACAGGG AGCGGGAGCG GGACCGCGAG 
AGTGACCGTG AGCATGAGCG TCCTCGCGAG AAAGAGCGGG ATAGAGAGAA GTACAGGGAA 
CGAGGAGACC GCGATAGTGA TCGTGAGCGC GACCGACCTC GTGAGAAGTA CAGGGAGAAG 
GGAACCGACC GCGGCGATAG CGACAGGGGC GGCCGCACCA CCGACAGCGA CCGGGAACGG 
AACCGGATCG ATCGGGATCG AGATCGGGAT CGGGAACGGG AACGCGAGAG GAGTGACCGT 
GACCGCGAGA GAACTGACCG CGAGAGGGAG CGGATTCGCG ACAAGTACCG TGATCGAGCT 
GATCGGGACA GCGATCGTGA TAGAGACAGT TACAAGGATC GTGATCGGGA GCGCGACCGC 
GACCGTGATA GAGACAGGGA GCGTGATCGT GGTGATCGTG GTGATCGAGG TGACCGAGAC 
CGGGACCGGG ACCGAGACCG AGACCGAGGA GATCGCGACA GGGTATCATC CTCCGGCTCA 
AGTCTCCTTA TCAAGAAGGA CAGAAAACCG TTGCCAGGAT CCGGAGAAAA AGAGCGGAGA 
CCATTGCCTA CTGGAGCGCC TCACGAAAAG GCACCGCATC CCCTCAAGGA ATCCCATTCC 
CCAATCCATC CGACTCCTGC TGCCACAACC AACGGCGGCG AGCCAAAGAA GAAAATCATT 
AAGCTCAAGT TGAAGAGTAG CGGAAGTGGT CTCGGAGGTG GTTCTGGTTC GTCATCGACG 
CCTGGTGCTG GTGGTGGTAG TGGATCTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU02052 ( XM_011394624.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011392926.1 Neurospora crassa OR74A transcription initiation factor TFIId 127kD subunit, variant (NCU02052), mRNA.

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CloneID	ONf02469
Clone ID Related Accession (Same CDS sequence)	XM_011394625.1 , XM_011394624.1
Accession Version	XM_011394624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	transcription initiation factor TFIId 127kD subunit, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCAGGAC TCGAGAATGG AGATGTGGCC CTTGCCAACA TGACGAACGC CCCCGATCCT 
GTGGCCGCTG CCGAGCCGTA CGCCATTTTG AGGCAAGAGG TGGAGCTAGA CATCAACTTC 
AGGGAGAAGA GCGTCAGCGG CATTTGCACA TTCAAGATTT ATCCCCTGGT TCCCGACCTT 
GAGGAACTCG TCCTAGACAC GCGGCAATGC GAAATCGACG TTTCAAACAT TACGGTGGAC 
GGCTACAAGA CACGGGCACA GGCTCATGAT CCTTACGATC GAATGGAGCC CCCCACGAAT 
GCGCAAATCG GTGCCACTCA GCATCACATA ATGAAGAAAT GGATGGCGCC ATTACTCCCA 
GAAACACGCC GCCAGGCTCC CATCGTAGAA AGGGCAGAGC AGCTTAATAT CAGCGTGCCG 
GCTGATGGGT CCCTCAGGAT CCGTCTCCGC CCAGATGCTC TCATCCAGGA TTCGGAGCCC 
AAACGCATTC TCAAGATCAA AACTCCCAAG TCGGTATCGG AAAATGCGAC AGGCCTCTCC 
GACCCTAATG AGGAACTCAC GGTGGTTGTG CCTTTCAGGT CCAAGAGAAT CAGAGATGGC 
CTGCACTTTG TCGGCGTTGA GGAAGGTGAC CTGAGATACC CACACGTTTA CACCCGGCAT 
TCGCTCGAGC CCGGTACGGC GTCTTGCATC TTCCCTTGCC TCGATGATCC CGGTTCGAGG 
CATTCCTGGA CAATCAAAAT CACATGCCCA AGAACGCTGG AGGATGCCTT CGCACCGCCT 
CTGGCTACCC AATCAGCACT GCTGACTGCT CTGGATGGCG GTCGGAAACG CAAAGCCTCT 
GATATTTCTG CACACCAGCC TGGCTCATCT TTCACCGAAG AGGATAAGCT GAGGGAGATT 
ACCGTGGTCT GCTCTGGCAA TCTCATTGGC GAGCAGATGC ACCCCAAGGA CGAGCGCAAG 
AAGACCATGA TTTTTGAGTG CGTCAACACC GCTGCTAGGC ACATCGGTTT CGCCGTTGGC 
CCCTTTGAGC ACGTTGATCT TGGGGCCGAG TTTAGAACTG AGGAGGCGGA CGAGAAGCTC 
GGGGCTAATG CAGCCAGAAT TCACGGCTAT TGTCTCCCTG GCCGTGTGGA TGAAGTTCGA 
TGGACATGCC AAGCCGTCGT GGCTGCTGCC GACTACTTCG CCCCTGAGTT CGGCCGTTAT 
CCTTATGAAG CCTACAAGAT GTGCTTTGTC GAAGACTTGG TTCACGACAC TGCGGTTGCA 
AACTCCTTAT CCTTGTGCAG TACGCGTTTG CTGTACCCCG AAACGATCAT AGATACGGAC 
ATCGAGGTTA CCAGGAAGCT CGTCCATGCC CTCGCCAGTC AGTACGTGGG GGTCCACATT 
GTGCCAAATG AGCGGTCCGA CACTTGGCTT ATCATCGGTC TCCAGTGGTT CATGACAGAT 
CTGTTCATGA GGACCATCTG CGGAAACAAC TGGTATCGTT TTCATATCAA GTCGCTTTCC 
GACAAGCTTG TAGAGGCGGA TGTGCGGAGA CCCTCCTTGC ATGCTCTGGG CGATTATCTT 
CACCTAGGTG ACTTTGAGCT CGAGTTTATG GCGCTCAAGG CGCCACTTGT CCTTTACATA 
CTCGATCAGC GCATGTCCAA GATTCCTGGC TCGACAGGTG TCGTCCGCGT TTTGTCGCAA 
ATGGTTTCGG CCGCTAATAT TTCCAACACG GACCCCAAGG CCACTACCCT CTCTTTCCAG 
GACTTCCGTC GATGGTGCGA GAAACGCAGT CAATACGTCC CTGATGAGCT TTGGAATCAA 
TACGTGTTTG GTGCGGGTTG CCCGAGACTG GATATTCACC AAAAGTTCAA CAAGAAGAAT 
CTCAACGTCG ACATCCAAAT TCTACAATAT CAGGGACCGA CCAAGAAAAG CATATCAAAG 
GATGAGTTCT GGAGGGAGTT GCAAGAGGAG ATACACCAGG TCTATGCCGG CGACGTTCCC 
AAGCTGTTCA CTGGTCCATT CACCGTCCGA ATTCACGAAG CAGACGGTAC GCCTTATGAG 
CACTACCAGC TCATTACCGA GAAAGATAAA GGTGGGACGA ATTTGCAGAT TGCGTACAAT 
ACAAAGTACA AGCGTCTGAA AAGAACCAAA AAGGCCATGG CCGCGGCTGC CAACAGCAAT 
AGTGACAAAC ACGAGATCCA GGAGGACGAT GTTGTCTACT TTAACATGTT GGGCGATGTC 
CTCACCAGCC AAAAGGATAT GGAGGACTGG GGGTTACAAG ACTGGGCTTC AGAGGTCCAA 
ACCAAGATGG ATCAAGAATC CTATGAATGG ATCCGGCTTG ATGCTAACAT GGAGTGGCTT 
TGCTTCATGA AGACTGATAT GCAGGAGTAC ATGTATCTGG CACAACTACA GCAGGATAGA 
GACGTCGTGG CCCACCAGGA CGCGATGCTC GCTTTCAAGA GGGAAAAGAG GCATGCGGTC 
CACTCGACGA TCGAGACGCG GACAGTGATG GACCGACGAT ATTACCATGG TGTCCGAGTG 
ATGGCATTGG AAGACCTCGC TAAACAGGCA CATCCTGACT TGAATTACAT CGGCATGGTC 
CATCTGATAC TCTGTTTCCG CAAGTTTTTC TGCAAGAAGG TACCAATTCC CGGCCGGGAG 
GGTGGCTATC ACTACCCGAT CGCTCCCAAC GATTTTACCG ACAAGGGCCA GTATGCCATC 
CAATGTGCGA TTCCCGCGGT GCTTGCACGG ACGACGGACC TGCAGGAAAA AGGAAGATGC 
TCTAAGAGGG CCCGACAGTT CATCATGGAG ATGCTCCTGT TTAATGACAA TTCGGAGAAT 
GAGTTTTCAG ACCAGTTCTA CATCGCCAAA CTCCTCGATG CTCTTACCAC CTCTGTGATT 
CCGCAGAAGG AGATTACCGA GGGATACCTC ATCAAAAACC TGGATCCTTT CGATGAAGAC 
GATGCGGAAT TCAAAAGTTT CATTGAGAAG ACCATTGAGG AGATCGACAA GTTTCGCAGG 
ATGGATGAAT GGACACTGAC ATACCAGAAC ATTTGGACGA CAACGGCTCT TCACTGCAAG 
ATGAGGCTCA TGAAGGCTCG TGTCATCCCT GAATCGGCCC TCGACTTTGT TCAGTACCTC 
CAGGACGACA ACATTGATCT TGTACGTATC AAGGCGTTTG AGTGCCTGGT TGAGCTTGGA 
ATGATCTCCA AACCCCCTAT TCTCAAGCTT CTACTTTCAT ACATGAGCAC CGATAGTTCG 
CCCTTCATCC GCGATCGATT GTTCAAGGTC TTTTGCCGGG GAATCGCTGC CATTGCGTTT 
GGCGAGAACA AAGCTTCGAC ACAGGAACTT GAAGCTCTTA ATGATGGAGG TCTCGTCATC 
GATCAAGGTG ACGCAGAGAT CCAACAACGG AAGCTCGATG CAGTACGCAG AGAAGACATT 
GCCCACGCGC TTAATGCGCT CAAGGAAGAG CTGAGGGGGA ACACGGAGAT GCAAATATCC 
ATATGGAAGG CATTTAATTC TCCCGTCATC GGTCTACAGG AGAAGCGCCA GCTATTGGAG 
CTCTGCTCGG CCATGTTCGA ACCAGAAGAT TCTCTACTTG TTACTATCAA GTATCCCATT 
GTCTGGAAGG CAACTCGCGA CCCGACAATT GTCGCCACTG CACCACAGAT CCTCGGAACT 
CCGCCCAAGA AGAGATGTGT CGTCAAGTTC AAGTCGGAGT ACCGGACGCT TCCTAAGAGT 
AAGGATATGG TTGCGATCGA AGCTCCACCG CCTCCGGCGC CATTACCCGC ACCGACACCG 
TCGACCAACT TGTCGGCTTC CAGGGTAGAG ATTAAGCCTC CTGAGAAGAA GACAATCGTA 
AAGCTACAAT CACGGCCTTC GTACATTGCG AAACCTCGGG AGTCACCAGT CCCAGAAGCT 
CCCCCACCAG TACGGAAGGA CTCTATCGCT ATGACCGTGC CTCCTCGGCC TTCTACGACT 
GCCGGTCCGC GCACTCCCTT GCCCACATCA GTTCCAAGTG CTCCCGCACC CAATACATCC 
GCCGCTCAGC CTAGCATCAA GACAGCTGTA CCAAAAGCGG CTTCACCTCC CCCCGTACCT 
GCACCCAAAC CCCAACAGTC CAACGACGTT GCCGTTCCAA GGCCTAGCAC CGTCAATTTG 
GGCAAGCCAA AGATCGTCAA GCCGGTCATC AAGCGCAAGA GTGACGAGTC ATTTGAGAAC 
CCTAGCCCGA AGAAGATCAT CAAGACCAAC AGCGACTCCT TCCGGAGCGA TCCTTCTCGC 
AATAGCTCAT TCAAGACAGA ACCCCCTCGC AGTGAGTCCT TTAAGAAAGA GGCTCCTCGA 
AGCGAAGCCA TCAGGGGCCA CTCTTTCCGC GAATCCGCCC CTCAGATTCC GCCTCCCCGC 
ACATCTTCAT TCTCCGTCCG TCCCAACGCC AATGGCCAGC CTTCCAAAGT CGTCACGCTA 
CCATTTAGGA AATGGACCAA GCTGTCTGCC CGCGCCCAGC GCAGCATCGA CTACGAACAA 
AGTCAGCGTG AAAAGCAAAT GCTTGCCGCC AGCGCCAGAG GCATTTCCGG CAGTCTTGCC 
ACACCCGTCC ACAAGCGATC GAGCGCCGCA GTAGGATTTG GCAGCGGTTC GGCGCCTTCA 
TCCCCTAAGA GCCGGATGCA GTCTCCTGCC GGTAACGGAT ACGGCTCGTC TGGATCGTAT 
GCTGAGAAGG AGCAGCGGAA TAAGGAGAGG GATAGAGACA GGGAACGGGA CAGAGATAGG 
GAGAGAGACA GCAGTAGCAA ATTCCGGGAG AAGGACCGCA ATGATCACGA CAGGGAGCGA 
TACCGTGGTG GTGCCGAGCG AGAAAAGGAG AAGTACAGGG AGCGGGAGCG GGACCGCGAG 
AGTGACCGTG AGCATGAGCG TCCTCGCGAG AAAGAGCGGG ATAGAGAGAA GTACAGGGAA 
CGAGGAGACC GCGATAGTGA TCGTGAGCGC GACCGACCTC GTGAGAAGTA CAGGGAGAAG 
GGAACCGACC GCGGCGATAG CGACAGGGGC GGCCGCACCA CCGACAGCGA CCGGGAACGG 
AACCGGATCG ATCGGGATCG AGATCGGGAT CGGGAACGGG AACGCGAGAG GAGTGACCGT 
GACCGCGAGA GAACTGACCG CGAGAGGGAG CGGATTCGCG ACAAGTACCG TGATCGAGCT 
GATCGGGACA GCGATCGTGA TAGAGACAGT TACAAGGATC GTGATCGGGA GCGCGACCGC 
GACCGTGATA GAGACAGGGA GCGTGATCGT GGTGATCGTG GTGATCGAGG TGACCGAGAC 
CGGGACCGGG ACCGAGACCG AGACCGAGGA GATCGCGACA GGGTATCATC CTCCGGCTCA 
AGTCTCCTTA TCAAGAAGGA CAGAAAACCG TTGCCAGGAT CCGGAGAAAA AGAGCGGAGA 
CCATTGCCTA CTGGAGCGCC TCACGAAAAG GCACCGCATC CCCTCAAGGA ATCCCATTCC 
CCAATCCATC CGACTCCTGC TGCCACAACC AACGGCGGCG AGCCAAAGAA GAAAATCATT 
AAGCTCAAGT TGAAGAGTAG CGGAAGTGGT CTCGGAGGTG GTTCTGGTTC GTCATCGACG 
CCTGGTGCTG GTGGTGGTAG TGGATCTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011392926.1
CDS	926..6538
Translation	MSGLENGDVALANMTNAPDPVAAAEPYAILRQEVELDINFREKSVSGICTFKIYPLVPDLEELVLDTRQCEIDVSNITVDGYKTRAQAHDPYDRMEPPTNAQIGATQHHIMKKWMAPLLPETRRQAPIVERAEQLNISVPADGSLRIRLRPDALIQDSEPKRILKIKTPKSVSENATGLSDPNEELTVVVPFRSKRIRDGLHFVGVEEGDLRYPHVYTRHSLEPGTASCIFPCLDDPGSRHSWTIKITCPRTLEDAFAPPLATQSALLTALDGGRKRKASDISAHQPGSSFTEEDKLREITVVCSGNLIGEQMHPKDERKKTMIFECVNTAARHIGFAVGPFEHVDLGAEFRTEEADEKLGANAARIHGYCLPGRVDEVRWTCQAVVAAADYFAPEFGRYPYEAYKMCFVEDLVHDTAVANSLSLCSTRLLYPETIIDTDIEVTRKLVHALASQYVGVHIVPNERSDTWLIIGLQWFMTDLFMRTICGNNWYRFHIKSLSDKLVEADVRRPSLHALGDYLHLGDFELEFMALKAPLVLYILDQRMSKIPGSTGVVRVLSQMVSAANISNTDPKATTLSFQDFRRWCEKRSQYVPDELWNQYVFGAGCPRLDIHQKFNKKNLNVDIQILQYQGPTKKSISKDEFWRELQEEIHQVYAGDVPKLFTGPFTVRIHEADGTPYEHYQLITEKDKGGTNLQIAYNTKYKRLKRTKKAMAAAANSNSDKHEIQEDDVVYFNMLGDVLTSQKDMEDWGLQDWASEVQTKMDQESYEWIRLDANMEWLCFMKTDMQEYMYLAQLQQDRDVVAHQDAMLAFKREKRHAVHSTIETRTVMDRRYYHGVRVMALEDLAKQAHPDLNYIGMVHLILCFRKFFCKKVPIPGREGGYHYPIAPNDFTDKGQYAIQCAIPAVLARTTDLQEKGRCSKRARQFIMEMLLFNDNSENEFSDQFYIAKLLDALTTSVIPQKEITEGYLIKNLDPFDEDDAEFKSFIEKTIEEIDKFRRMDEWTLTYQNIWTTTALHCKMRLMKARVIPESALDFVQYLQDDNIDLVRIKAFECLVELGMISKPPILKLLLSYMSTDSSPFIRDRLFKVFCRGIAAIAFGENKASTQELEALNDGGLVIDQGDAEIQQRKLDAVRREDIAHALNALKEELRGNTEMQISIWKAFNSPVIGLQEKRQLLELCSAMFEPEDSLLVTIKYPIVWKATRDPTIVATAPQILGTPPKKRCVVKFKSEYRTLPKSKDMVAIEAPPPPAPLPAPTPSTNLSASRVEIKPPEKKTIVKLQSRPSYIAKPRESPVPEAPPPVRKDSIAMTVPPRPSTTAGPRTPLPTSVPSAPAPNTSAAQPSIKTAVPKAASPPPVPAPKPQQSNDVAVPRPSTVNLGKPKIVKPVIKRKSDESFENPSPKKIIKTNSDSFRSDPSRNSSFKTEPPRSESFKKEAPRSEAIRGHSFRESAPQIPPPRTSSFSVRPNANGQPSKVVTLPFRKWTKLSARAQRSIDYEQSQREKQMLAASARGISGSLATPVHKRSSAAVGFGSGSAPSSPKSRMQSPAGNGYGSSGSYAEKEQRNKERDRDRERDRDRERDSSSKFREKDRNDHDRERYRGGAEREKEKYRERERDRESDREHERPREKERDREKYRERGDRDSDRERDRPREKYREKGTDRGDSDRGGRTTDSDRERNRIDRDRDRDRERERERSDRDRERTDRERERIRDKYRDRADRDSDRDRDSYKDRDRERDRDRDRDRERDRGDRGDRGDRDRDRDRDRDRGDRDRVSSSGSSLLIKKDRKPLPGSGEKERRPLPTGAPHEKAPHPLKESHSPIHPTPAATTNGGEPKKKIIKLKLKSSGSGLGGGSGSSSTPGAGGGSGSR

Target ORF information:

RefSeq Version	XM_011394624.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A transcription initiation factor TFIId 127kD subunit, variant (NCU02052), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394624.1

ORF Insert Sequence:

ATGTCAGGAC TCGAGAATGG AGATGTGGCC CTTGCCAACA TGACGAACGC CCCCGATCCT 
GTGGCCGCTG CCGAGCCGTA CGCCATTTTG AGGCAAGAGG TGGAGCTAGA CATCAACTTC 
AGGGAGAAGA GCGTCAGCGG CATTTGCACA TTCAAGATTT ATCCCCTGGT TCCCGACCTT 
GAGGAACTCG TCCTAGACAC GCGGCAATGC GAAATCGACG TTTCAAACAT TACGGTGGAC 
GGCTACAAGA CACGGGCACA GGCTCATGAT CCTTACGATC GAATGGAGCC CCCCACGAAT 
GCGCAAATCG GTGCCACTCA GCATCACATA ATGAAGAAAT GGATGGCGCC ATTACTCCCA 
GAAACACGCC GCCAGGCTCC CATCGTAGAA AGGGCAGAGC AGCTTAATAT CAGCGTGCCG 
GCTGATGGGT CCCTCAGGAT CCGTCTCCGC CCAGATGCTC TCATCCAGGA TTCGGAGCCC 
AAACGCATTC TCAAGATCAA AACTCCCAAG TCGGTATCGG AAAATGCGAC AGGCCTCTCC 
GACCCTAATG AGGAACTCAC GGTGGTTGTG CCTTTCAGGT CCAAGAGAAT CAGAGATGGC 
CTGCACTTTG TCGGCGTTGA GGAAGGTGAC CTGAGATACC CACACGTTTA CACCCGGCAT 
TCGCTCGAGC CCGGTACGGC GTCTTGCATC TTCCCTTGCC TCGATGATCC CGGTTCGAGG 
CATTCCTGGA CAATCAAAAT CACATGCCCA AGAACGCTGG AGGATGCCTT CGCACCGCCT 
CTGGCTACCC AATCAGCACT GCTGACTGCT CTGGATGGCG GTCGGAAACG CAAAGCCTCT 
GATATTTCTG CACACCAGCC TGGCTCATCT TTCACCGAAG AGGATAAGCT GAGGGAGATT 
ACCGTGGTCT GCTCTGGCAA TCTCATTGGC GAGCAGATGC ACCCCAAGGA CGAGCGCAAG 
AAGACCATGA TTTTTGAGTG CGTCAACACC GCTGCTAGGC ACATCGGTTT CGCCGTTGGC 
CCCTTTGAGC ACGTTGATCT TGGGGCCGAG TTTAGAACTG AGGAGGCGGA CGAGAAGCTC 
GGGGCTAATG CAGCCAGAAT TCACGGCTAT TGTCTCCCTG GCCGTGTGGA TGAAGTTCGA 
TGGACATGCC AAGCCGTCGT GGCTGCTGCC GACTACTTCG CCCCTGAGTT CGGCCGTTAT 
CCTTATGAAG CCTACAAGAT GTGCTTTGTC GAAGACTTGG TTCACGACAC TGCGGTTGCA 
AACTCCTTAT CCTTGTGCAG TACGCGTTTG CTGTACCCCG AAACGATCAT AGATACGGAC 
ATCGAGGTTA CCAGGAAGCT CGTCCATGCC CTCGCCAGTC AGTACGTGGG GGTCCACATT 
GTGCCAAATG AGCGGTCCGA CACTTGGCTT ATCATCGGTC TCCAGTGGTT CATGACAGAT 
CTGTTCATGA GGACCATCTG CGGAAACAAC TGGTATCGTT TTCATATCAA GTCGCTTTCC 
GACAAGCTTG TAGAGGCGGA TGTGCGGAGA CCCTCCTTGC ATGCTCTGGG CGATTATCTT 
CACCTAGGTG ACTTTGAGCT CGAGTTTATG GCGCTCAAGG CGCCACTTGT CCTTTACATA 
CTCGATCAGC GCATGTCCAA GATTCCTGGC TCGACAGGTG TCGTCCGCGT TTTGTCGCAA 
ATGGTTTCGG CCGCTAATAT TTCCAACACG GACCCCAAGG CCACTACCCT CTCTTTCCAG 
GACTTCCGTC GATGGTGCGA GAAACGCAGT CAATACGTCC CTGATGAGCT TTGGAATCAA 
TACGTGTTTG GTGCGGGTTG CCCGAGACTG GATATTCACC AAAAGTTCAA CAAGAAGAAT 
CTCAACGTCG ACATCCAAAT TCTACAATAT CAGGGACCGA CCAAGAAAAG CATATCAAAG 
GATGAGTTCT GGAGGGAGTT GCAAGAGGAG ATACACCAGG TCTATGCCGG CGACGTTCCC 
AAGCTGTTCA CTGGTCCATT CACCGTCCGA ATTCACGAAG CAGACGGTAC GCCTTATGAG 
CACTACCAGC TCATTACCGA GAAAGATAAA GGTGGGACGA ATTTGCAGAT TGCGTACAAT 
ACAAAGTACA AGCGTCTGAA AAGAACCAAA AAGGCCATGG CCGCGGCTGC CAACAGCAAT 
AGTGACAAAC ACGAGATCCA GGAGGACGAT GTTGTCTACT TTAACATGTT GGGCGATGTC 
CTCACCAGCC AAAAGGATAT GGAGGACTGG GGGTTACAAG ACTGGGCTTC AGAGGTCCAA 
ACCAAGATGG ATCAAGAATC CTATGAATGG ATCCGGCTTG ATGCTAACAT GGAGTGGCTT 
TGCTTCATGA AGACTGATAT GCAGGAGTAC ATGTATCTGG CACAACTACA GCAGGATAGA 
GACGTCGTGG CCCACCAGGA CGCGATGCTC GCTTTCAAGA GGGAAAAGAG GCATGCGGTC 
CACTCGACGA TCGAGACGCG GACAGTGATG GACCGACGAT ATTACCATGG TGTCCGAGTG 
ATGGCATTGG AAGACCTCGC TAAACAGGCA CATCCTGACT TGAATTACAT CGGCATGGTC 
CATCTGATAC TCTGTTTCCG CAAGTTTTTC TGCAAGAAGG TACCAATTCC CGGCCGGGAG 
GGTGGCTATC ACTACCCGAT CGCTCCCAAC GATTTTACCG ACAAGGGCCA GTATGCCATC 
CAATGTGCGA TTCCCGCGGT GCTTGCACGG ACGACGGACC TGCAGGAAAA AGGAAGATGC 
TCTAAGAGGG CCCGACAGTT CATCATGGAG ATGCTCCTGT TTAATGACAA TTCGGAGAAT 
GAGTTTTCAG ACCAGTTCTA CATCGCCAAA CTCCTCGATG CTCTTACCAC CTCTGTGATT 
CCGCAGAAGG AGATTACCGA GGGATACCTC ATCAAAAACC TGGATCCTTT CGATGAAGAC 
GATGCGGAAT TCAAAAGTTT CATTGAGAAG ACCATTGAGG AGATCGACAA GTTTCGCAGG 
ATGGATGAAT GGACACTGAC ATACCAGAAC ATTTGGACGA CAACGGCTCT TCACTGCAAG 
ATGAGGCTCA TGAAGGCTCG TGTCATCCCT GAATCGGCCC TCGACTTTGT TCAGTACCTC 
CAGGACGACA ACATTGATCT TGTACGTATC AAGGCGTTTG AGTGCCTGGT TGAGCTTGGA 
ATGATCTCCA AACCCCCTAT TCTCAAGCTT CTACTTTCAT ACATGAGCAC CGATAGTTCG 
CCCTTCATCC GCGATCGATT GTTCAAGGTC TTTTGCCGGG GAATCGCTGC CATTGCGTTT 
GGCGAGAACA AAGCTTCGAC ACAGGAACTT GAAGCTCTTA ATGATGGAGG TCTCGTCATC 
GATCAAGGTG ACGCAGAGAT CCAACAACGG AAGCTCGATG CAGTACGCAG AGAAGACATT 
GCCCACGCGC TTAATGCGCT CAAGGAAGAG CTGAGGGGGA ACACGGAGAT GCAAATATCC 
ATATGGAAGG CATTTAATTC TCCCGTCATC GGTCTACAGG AGAAGCGCCA GCTATTGGAG 
CTCTGCTCGG CCATGTTCGA ACCAGAAGAT TCTCTACTTG TTACTATCAA GTATCCCATT 
GTCTGGAAGG CAACTCGCGA CCCGACAATT GTCGCCACTG CACCACAGAT CCTCGGAACT 
CCGCCCAAGA AGAGATGTGT CGTCAAGTTC AAGTCGGAGT ACCGGACGCT TCCTAAGAGT 
AAGGATATGG TTGCGATCGA AGCTCCACCG CCTCCGGCGC CATTACCCGC ACCGACACCG 
TCGACCAACT TGTCGGCTTC CAGGGTAGAG ATTAAGCCTC CTGAGAAGAA GACAATCGTA 
AAGCTACAAT CACGGCCTTC GTACATTGCG AAACCTCGGG AGTCACCAGT CCCAGAAGCT 
CCCCCACCAG TACGGAAGGA CTCTATCGCT ATGACCGTGC CTCCTCGGCC TTCTACGACT 
GCCGGTCCGC GCACTCCCTT GCCCACATCA GTTCCAAGTG CTCCCGCACC CAATACATCC 
GCCGCTCAGC CTAGCATCAA GACAGCTGTA CCAAAAGCGG CTTCACCTCC CCCCGTACCT 
GCACCCAAAC CCCAACAGTC CAACGACGTT GCCGTTCCAA GGCCTAGCAC CGTCAATTTG 
GGCAAGCCAA AGATCGTCAA GCCGGTCATC AAGCGCAAGA GTGACGAGTC ATTTGAGAAC 
CCTAGCCCGA AGAAGATCAT CAAGACCAAC AGCGACTCCT TCCGGAGCGA TCCTTCTCGC 
AATAGCTCAT TCAAGACAGA ACCCCCTCGC AGTGAGTCCT TTAAGAAAGA GGCTCCTCGA 
AGCGAAGCCA TCAGGGGCCA CTCTTTCCGC GAATCCGCCC CTCAGATTCC GCCTCCCCGC 
ACATCTTCAT TCTCCGTCCG TCCCAACGCC AATGGCCAGC CTTCCAAAGT CGTCACGCTA 
CCATTTAGGA AATGGACCAA GCTGTCTGCC CGCGCCCAGC GCAGCATCGA CTACGAACAA 
AGTCAGCGTG AAAAGCAAAT GCTTGCCGCC AGCGCCAGAG GCATTTCCGG CAGTCTTGCC 
ACACCCGTCC ACAAGCGATC GAGCGCCGCA GTAGGATTTG GCAGCGGTTC GGCGCCTTCA 
TCCCCTAAGA GCCGGATGCA GTCTCCTGCC GGTAACGGAT ACGGCTCGTC TGGATCGTAT 
GCTGAGAAGG AGCAGCGGAA TAAGGAGAGG GATAGAGACA GGGAACGGGA CAGAGATAGG 
GAGAGAGACA GCAGTAGCAA ATTCCGGGAG AAGGACCGCA ATGATCACGA CAGGGAGCGA 
TACCGTGGTG GTGCCGAGCG AGAAAAGGAG AAGTACAGGG AGCGGGAGCG GGACCGCGAG 
AGTGACCGTG AGCATGAGCG TCCTCGCGAG AAAGAGCGGG ATAGAGAGAA GTACAGGGAA 
CGAGGAGACC GCGATAGTGA TCGTGAGCGC GACCGACCTC GTGAGAAGTA CAGGGAGAAG 
GGAACCGACC GCGGCGATAG CGACAGGGGC GGCCGCACCA CCGACAGCGA CCGGGAACGG 
AACCGGATCG ATCGGGATCG AGATCGGGAT CGGGAACGGG AACGCGAGAG GAGTGACCGT 
GACCGCGAGA GAACTGACCG CGAGAGGGAG CGGATTCGCG ACAAGTACCG TGATCGAGCT 
GATCGGGACA GCGATCGTGA TAGAGACAGT TACAAGGATC GTGATCGGGA GCGCGACCGC 
GACCGTGATA GAGACAGGGA GCGTGATCGT GGTGATCGTG GTGATCGAGG TGACCGAGAC 
CGGGACCGGG ACCGAGACCG AGACCGAGGA GATCGCGACA GGGTATCATC CTCCGGCTCA 
AGTCTCCTTA TCAAGAAGGA CAGAAAACCG TTGCCAGGAT CCGGAGAAAA AGAGCGGAGA 
CCATTGCCTA CTGGAGCGCC TCACGAAAAG GCACCGCATC CCCTCAAGGA ATCCCATTCC 
CCAATCCATC CGACTCCTGC TGCCACAACC AACGGCGGCG AGCCAAAGAA GAAAATCATT 
AAGCTCAAGT TGAAGAGTAG CGGAAGTGGT CTCGGAGGTG GTTCTGGTTC GTCATCGACG 
CCTGGTGCTG GTGGTGGTAG TGGATCTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU02052 cDNA ORF clone, Neurospora crassa OR74A

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