NCU02858 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU02858
Entrez Gene ID	3880141
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_958899.3	XP_963992.3	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU02858 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02858 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12638	XM_958899.3 Latest version!	Neurospora crassa OR74A hypothetical protein (NCU02858), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU02858 ( XM_958899.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_963992.3 Neurospora crassa OR74A hypothetical protein (NCU02858), mRNA.

Price & Availability↑

CloneID	ONf12638
Clone ID Related Accession (Same CDS sequence)	XM_958899.3
Accession Version	XM_958899.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_958899.2.

ATGGTTCCGG AGGGCCCTAC TGCTCCCGAA ACGCTTACCG GCTTTAGCCC AGCTCAGCTT 
CATCCTGAAA GCTTCCCGCC CTTATATGTC AATCCAGACA GAACTCCAGT TTCCGGCACG 
CCTGGTGGTG TAGCGCCATC GTCTGCTGGC GAGGGACAGT CGGTTGAACC AGAAAAGCAC 
GAACCCGCTG TCCCGGAATT TGCGCAAGAG AATCCAGCGA ATGACAACAA CGAAGACAAC 
GGTCTGGGAG ATGGTGAACG GCAGAATATC GACGCCTGGG CAAGAGGAGA AGCTGAACTC 
ACAACCCATG TTGAGCCCGA TGATGAAGCA CCTGCAGCCG GGTCCCCGGA TATTCCCGAG 
TTATGTAATG TTCTACGTCC ACCTCGTTCA TTGAACTCCT CGGACAATAA TTCGTCGGAT 
ACATCAGAGG TTGCTGCTAA AGGCCAGTTT CAACACGAAT CCCTGGACGC GTGGCAAAGA 
CGGTTACAAG ACGAGTCCCG GGGATTTCCA GAGCCCCCTA TGGGTACGAT GGCCTCTATG 
TTTGATTATA CTCCTTTTTC CGAGACTGCT TCCAACGCAC CTGCGTCCAA AGACACTCCG 
CCCAACGATT TGCCCGGCGA TAGAATCGCA GAACCGACGG ATGCTGCCTC ATCTTCGGAC 
GGCACTCTCT TCGATGCCGA GACCACCCCG CTGGCCACCC CGCCGCACAA TGGTAACGGC 
GTTATTCCCA CATTAATTAG CTCATTTGAC AAACGCACGT TCTTTGAAAA CCTGTCGAGA 
GTTTTCAAAA AGGGAAGAAA ACGAGCAGAG AAACTTCGTG AGCGGATCAC GTTGCTTGAA 
ACTGGGATTG GCGGTGCTGT TCCAACGTCA ATTGATGAAC AGAAGAACAA AGACCACGTC 
CAACACATGC CTGAAGAGAG AAGCCGCTAT AACAGTATCA TGATCAAATT ATTAAAAGAA 
GGATACAATG TTCCAATACC GTGGGATGCA CTCGAAGAGC CGCAAAATGA AGAAGAAGAT 
ATCAGCAGCT CCGCGGGACC TCTCATGAAG CAAGCACAAG CGGGAGTCTC TCCCCCGCAA 
AACATCTCTG AAGCTGGCAA AGAGGAATCA CTTGAAGCTG GAAGAGCCTT GGACAAATTA 
CTCGATGAAA AACTGGGAGA AGCCAAAAAC GCTCAGAAAA AGGTAGCTAA AGGGGAAACG 
AAGACCGATG GCCCCCAAGT TAACCCGATT GAGCACCAGA AGCCAAATGT TGCTAAGACA 
CCGGATAACC CCGAGAAGCC AGAGGTCCTC GAGGCAAGGG GAGAACAAGC TCCGGAGTAC 
AAGACCCTCG CAGAAGAAAG GCGTAAAGCC GAGCGGGAAG ATGCGGACCG CAAGCCAGCG 
GAGCAAACTG TCAAGGGTGA GGAAGAGGAA ACCGCAAAGA ATGACGGGGA TGTCAAGCCC 
AAAACATTGG AGGAGCAGCA AAAAGCTAAG CAAGAAGAAG AGGATCGCAA ACTAGCACAG 
CAGATTGCCA AAACCCAACA AGAAGAAGCC GCAAAAGCTT CCGAGGATGC AAAGCGCAGA 
CAGGCAGAGG AAGCTGACGA TCTAGCCAAG TCCACCGAGG CCGAAAAGGA CAAAGCCCTC 
GGAGAGCGGA TCAAGGCTGC TGAAGCTGAA ATGCTCAAGG AAATTGGACA CGCAAGGGCT 
AAAGTTGCAG AGGCCGAGAG GCTCACAAAG ACCGAAAAAA ACAAGGTCAA AACGGCCAAT 
TCTGAAATGT CCGTAGCCCG CATAGATGCC GAGGCTCTTG AAGAAAAACG ACGCAAAGCC 
GCGCAAGAGG GCAAAGCCAA AGACGCTGAA GCGCTTGAAG ACAGGAGGCG CAAAGCACTT 
GAATTGGCCG AAAAAGCTGC GGTTGCAGAA AAAGAAGCGT CTGAAAAGGT CAAGGTGTAT 
CAAGAGGCGG AAGACAAAGC CCGTGAAGAG CTCAGACTCC TTGAGCTTGT GAGGCAGGAC 
GCCGTCAAAG CGGAGAAGGC CAAGGCTGCT GAACTTGGGA ATTGGAACGT GGCCGTGAAC 
GAAAAGGCAG CTGCAGCCGA AAGAAGAAGG ATCGTTCGCG AGGCAGAAGA GGCTACTTTG 
GCTAAAACCA CCGTCGAGAA AACCCCCAAG ACTTTCACTC CAGGAGGAAA ACTCACCTCT 
GTTGCTGCAT CGAGAGGTGA TGTGTCGAGG ACAAGAGACA ATCACAAATT TGTGCCATTT 
TTGAAGACTT CGAAAGTTGC TGCAGCAAGA AGTGAAGCCG CCAAAGATGC CGATCGCAAG 
CACGCAGCGG CCGGAGCTGA GAAGCGCCAG GCACTCGAGG CTGCTGGGGC TGAAAAGCGC 
AAAGAGATCA AGAAGGGGAA TCAGCAGCCG GGCGTTGTGA AAGCTTCCGA AGCGAACGTT 
CCCAAGTCTG CTGATCCGGC CATTTCCAAA TCTCCTGAAG CGAGTATTCC CACAGCTCTG 
ACAGAAGCAG AGAAACCAAG AAACTTTTAC GAGACCATAA GGAGGCGCAG GGAATGGGAA 
GCTCAACGGG AGGAGGCTAA GAAAAAAGAC GAGGCTGAAA AAATCATTAA ACCCCCTGAA 
GCCGATATTT CCAAGCCTTT CGAAGCGAAC GTTCCCAAGC CTGCTGATCC GGTCATTTCC 
AAATCCCCTG AAGCGAGTAT TCCCACGCCT CTAACAGAAG TAGAGAAACC AAGAAACTTT 
TACGAGACCA TAAGGAGGCG CAGGGAATGG GAAGCTCAAC GGGAGGAGGC TAAGAAAACA 
GACGAGGCTG AAAAAATCAT TAAACCCCCT AAAGCCGACA TTTCCAAGCC TTCCGAAGCG 
AACGTTCACA AGGCTCCTGA AAAAATCAAT AAACCCCCTG AAGCCGATAT TTCCAAGCCT 
TCCGAAGCGA ACGTTCACAA GCCTGCTGAT CCGGCCATTT CCAAATCTCC TGAAGCGAGT 
ATTCCCACAC CTCTAACAGA AGCAGAGAAA CAAAGAAACT TTTACGAGGC CATAAGGAGG 
CGCAGGGAAT GGGAAGCTAA ACGGGAGGAG GCTAAGAAAA AAGATGAGGA AACACGACGG 
GACTGGTTGG AGGAGAAAAA GAAGCAAATG CATGCCAACG ACAAACCTGC TTCTACTCAG 
CAGGCGAAGG TGGAAGAGGC ATGGCCCAGT TTGCCCTCTA CATCACAGCA GGTGAAGCCT 
GAAGCTCTGG GTGCTGCCCC TCCATCAGTC AAACTTCCCA AAATTATCAA GATTGCCCCG 
CCTAAAGAGA AGTCTCAAAG CTCCGCTAAG GCAAACCCGC AGCAGAAGCC TCAATCGCCT 
ACCCGAAAGC CGCAGCAAGC CTGGTCTCAA CCGAAGCCAG ATGGCAATGA TCGCGGAAAG 
ACCCAGACTC TGGTACAGGT GAAATCGCAA GAAAAAACTC AAGAGCAGGC TCAAGCTTCG 
GCTGCGGAAA ACACTCAGGG TCAATCTCAG AAGACCATGA AAGGCCCGGC GCCAAAGACG 
CATCCCGTTC CAGCTAAGGA GAAGCCACAG CCTCCAGCTC AGAAGAAGCC ACAGGATCCA 
GTTAAGAAGC AACCACAGTC CCAAGCACAG AAGAAGTCAC AGCAACCACC TCAACGAAAG 
GATGAAGCTC CAGCTCAGCA AAAGCCTCAA GTTCCAGCCG AGGAGAAACC CCAGCCTTCA 
CCTCAGAAGA AGCCACAGCA ACCACCCCAA CAGAAGAGTC AAGCTCCTGC CCGAGTAGAA 
AAACCGGAAC CTTCTGCCTG GAAGAAAAGG ATTAACAATT TGAATGCTAA AATCTCTGAA 
GAAAGTACTC ATACAAAGTC TCAGCCGCCT CGACCCTCAA TTGTATTTGG GGAGAAAAAG 
AACGAATTGC AGAAGGGAAA CAGCGCAGAT CAGACCCAGG CTGAAGTTCC AACTGACAAT 
AAGCCTCAAC TTGAGCCTAC ATCAAGCAAA ACAAAGCAAG ATGTTGGACC TGAAGCCACT 
CCAGCTACAA AAATGGACGA CAAAAAGGTC GAGGAGACAC TATACGTTCC ACCACAGCAT 
CAAGAAACGG TTGATACTAA GGCGCAAGAA GACCAGCTGA AGGCCCAGAA AGTCGAGACT 
GAAAGACTTG AGCACGAGAA AGCCGAGCAA GAACGCGTTG CACGAGAGAA GGCAGAAAGG 
GCGGAGCGCG AAAAGGCTGA GCGTGAAAAA GCTGAACGTG AGCAGGTCGC ACTCGAGAAA 
GCGCGCGAGA AAGCCGAACA AGAAAAAGCT GAGCGTGAAA AAGCCGAGCG AGAAAAGGCC 
GAACGGGAGC GTGTTGAACG TGAGAAGGCG CGCGAGAAGC TTGAGCAGGA GCGCATCGCA 
CGTGAAAAGG CTGAACTCGA GAAAGCTGAA CGTGAGCGGA TTGCAGCTGA GGAGGCGCGC 
AAGAAAGCTG AACTTGAGAA AGCTGAACTT GAGAAAGCTG AACGTGAGCG GATCGCAGCT 
GAGAAGGCGC GCAAGAAAGC TGAACTTGAG AAAGCTGAAC TTGAGAAAGC TGAACTTGAG 
AAAGCTGAAC GTGAGCGGGT CGCAGCTGAG AAGGCGCGCA AGAAAGCTGA GCAAGAAAAA 
GCCGAACAGG AGCGAGTTGA ACGTGAGAAG GCGCAGGAGA AAGCCCTACA AGAAAAGGCT 
GAGCAGGAGC GCATCGCACG CAAAAAGGCT GAACAGGAAA AGGCTGAGCG CCGAAAGGCC 
GATCTCGAGA AAGCTGAACG TGAGCGGGTC GCCCTTGAGG AGGCGCGCAA AAAGGCGGAA 
GAAGAAAAGG CTGAGCAGAA ACGCATTTCA CGCAAGAAGG CTGAGCTTGA GAAGGCCAAA 
CAGGAAAAGG CTGAGCGCGA AAAGGCTGAT CGTGAGAGAG CTAAGCAAGA AAAGGCTGAG 
CAGGAGCGTA TATCACGAGA GAAGGCTGAA CATGAGAAAG CTGAGCGTGA CAAGGCCGAG 
CAGGAGCGCG TTGCCCGTGA ACAGGCCGAA CGTAAGCAGG CCGAGCAGGA GCGGGTCGCA 
CGCGAGAAAG CCGAACATGA AAAGAGTGAG CGCGGAAAGA TCCAACAGGA ACGGCTTGCA 
CACGAGAGGG CTGAACACCA GAAAGCCGAG CGCGAGAAAG CCGAGCATGA GGAAGCTAAG 
CGCCAGGTAT TCGAACGTGA ATTAGCCAAT CGTCAAAGGG TTGCTCGCGA GACGGCAGAG 
CGTGAAGAAG CCGAACGCGA GAGATTGATG CACGAAAAAT TCAAGGCTGA AGAATTCGAG 
CGGCACAACC TCGCGGCCCA AAGGATCGCA CGTGAAATGG CCGAATATGA AAGGGTTAAA 
GAATGTGGAC CCGAGGATAA GTATATGGAA GCTGAAAAAC CCAAGCCAGT TGTCCAGAGT 
GGAACTGCAA ACCCGGTGGT CAAGGAAAAG GAGGAGAGAA CCCATGCTGG CCAGCCAATT 
TTCAATCTTG ATGGCTCGGA ACCAAAGCAT AAGGATAAAA TGCTCCAAGT TTGCTTGAAC 
TTGAAAGCAA AGAGGCTTGA ATTGGTTGAA AGCGCCGAGG AGTATGGTGA AGCAGCGAAG 
CGCCAGAACG AGAAGATGGA GGAACTTGTC CATCTGAGGG ATCACAATCT CATGATCAGA 
GAGCAAGAGC TTCAGAAGAT AAAGCTAATT GATGAACAAC TGGAGAAGGA AGTGCGCGCT 
ATACGTAGAG TAATCGCAGA AGAGCGACGA AAGATAGAAG CGAAGCAGCG GATGCTTGAT 
GCGGTGCAGG CGGAATTGAA GGCTGAAAAA CCTCTTGACT GGGTTCCATT CATTGTGTCC 
TCGGCCCAAC CCAACCTGAA CCGGGAGCAA GGACATGAAA GGGGGAGGGA AAGGGAGCGC 
CGCTCTGCGG TGGGCTTGAG CAGTCAAATT CCAGGAGCAC CTGAGAATTA TGGCGAGGAA 
TACCCGCTGG AAGGACATCC CGCTTGGGGA CATGTACCCG AGCAGGACCA GTATCCTTCA 
GTTGACGCTC CGTCTTGGAA TTCGTATCCT TTTAACGAAT CCAAAAATTC AGCACCAGAA 
CAGGAGCAGT CAGGAGGACC TAGAAACCCG CTACCCGAAG CACCAAGGTC ACTTGGAGTC 
CCCCAGAAAG CTGTGATTCT TCCTACAGAA GACTATGATA CATCTCAGGT ATCAAGACTA 
CCCGCAGAGT TTCAAAGCAC GATCCCAGGC CAGACTCACG TGCAGAACTT CGAAAACCAG 
CCCCCAGAAA CCGGCTTCGC GGGCATGTTC TGCATGAATT GCCACTGGGT ATTCGAAAAG 
CTGCGTTGTT CCTGCACCTG GGGCTCACTC AGCCGAGCTC GTCCCCTTCT CTGCCAATGG 
TGCGGATGGG AACACGGCTT CCTCTATTGT CCCACTCATC GTCTAAGCTT TTGGGACCAT 
ACCTCTGTGC CTGTTCAACC TCCTGCTGAA CTGACTCGGG TCTCGCATCC AACATCATCA 
TATCAAAGTG GCTACCAAGG ACAAACATAC GTGCCCCAAC CGCAGTGGAA TGCGCCTCCA 
CCGCAGTGGA ATGCGCCTCC ACCGCATTTA AACGCACCAC AGCAAGGCTT GCATGAATTC 
CAACCACAAT TCAACCCATC CCAAGGACCT TTGATGTCGC CACGGGAACC TGTATGGGTG 
CACAATGATG CAATTCCTGA ACCAAATCTA CAAGGGCCGT ATACTCAACC AAACCATCAT 
CGACAACTTT ACGACCCTCG CAACCCAAGC AGGTCACAAA ATGAGCCGTA TACTCAACCA 
AACCATCATC GACAACTTTA CGACCCTCGC AACCCAAGCA GGTCACAAAA TGAGCCGTAT 
CCGTCAGGTT CTAATCCCCC AAAGGGTTCA AGGGCCGAGA GGCCTTCGAG GCCCAGAAGG 
ACGAGCAGTT CACACAGTGG CCCGAATCAG CCAGGGTTGG GGCCACGCCT TTGGAGTCCA 
AACCAAAAGA AGTTGTTGGA CTCGTTCAAG GGAGATGGTG ATCGTGGAGA ACAAGTCCCT 
TCGCAGGCTC CTGTTGCGGA AGGGAATATC GTAACAAGCT CGACGCTTCC GAGTGTCCAG 
GAGGAGACCA AGTCCGCAGG AAGAGGTCGT CAAAGAAAAC ACCGCAACTC AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_963992.3
CDS	1003..8079
Translation	MVPEGPTAPETLTGFSPAQLHPESFPPLYVNPDRTPVSGTPGGVAPSSAGEGQSVEPEKHEPAVPEFAQENPANDNNEDNGLGDGERQNIDAWARGEAELTTHVEPDDEAPAAGSPDIPELCNVLRPPRSLNSSDNNSSDTSEVAAKGQFQHESLDAWQRRLQDESRGFPEPPMGTMASMFDYTPFSETASNAPASKDTPPNDLPGDRIAEPTDAASSSDGTLFDAETTPLATPPHNGNGVIPTLISSFDKRTFFENLSRVFKKGRKRAEKLRERITLLETGIGGAVPTSIDEQKNKDHVQHMPEERSRYNSIMIKLLKEGYNVPIPWDALEEPQNEEEDISSSAGPLMKQAQAGVSPPQNISEAGKEESLEAGRALDKLLDEKLGEAKNAQKKVAKGETKTDGPQVNPIEHQKPNVAKTPDNPEKPEVLEARGEQAPEYKTLAEERRKAEREDADRKPAEQTVKGEEEETAKNDGDVKPKTLEEQQKAKQEEEDRKLAQQIAKTQQEEAAKASEDAKRRQAEEADDLAKSTEAEKDKALGERIKAAEAEMLKEIGHARAKVAEAERLTKTEKNKVKTANSEMSVARIDAEALEEKRRKAAQEGKAKDAEALEDRRRKALELAEKAAVAEKEASEKVKVYQEAEDKAREELRLLELVRQDAVKAEKAKAAELGNWNVAVNEKAAAAERRRIVREAEEATLAKTTVEKTPKTFTPGGKLTSVAASRGDVSRTRDNHKFVPFLKTSKVAAARSEAAKDADRKHAAAGAEKRQALEAAGAEKRKEIKKGNQQPGVVKASEANVPKSADPAISKSPEASIPTALTEAEKPRNFYETIRRRREWEAQREEAKKKDEAEKIIKPPEADISKPFEANVPKPADPVISKSPEASIPTPLTEVEKPRNFYETIRRRREWEAQREEAKKTDEAEKIIKPPKADISKPSEANVHKAPEKINKPPEADISKPSEANVHKPADPAISKSPEASIPTPLTEAEKQRNFYEAIRRRREWEAKREEAKKKDEETRRDWLEEKKKQMHANDKPASTQQAKVEEAWPSLPSTSQQVKPEALGAAPPSVKLPKIIKIAPPKEKSQSSAKANPQQKPQSPTRKPQQAWSQPKPDGNDRGKTQTLVQVKSQEKTQEQAQASAAENTQGQSQKTMKGPAPKTHPVPAKEKPQPPAQKKPQDPVKKQPQSQAQKKSQQPPQRKDEAPAQQKPQVPAEEKPQPSPQKKPQQPPQQKSQAPARVEKPEPSAWKKRINNLNAKISEESTHTKSQPPRPSIVFGEKKNELQKGNSADQTQAEVPTDNKPQLEPTSSKTKQDVGPEATPATKMDDKKVEETLYVPPQHQETVDTKAQEDQLKAQKVETERLEHEKAEQERVAREKAERAEREKAEREKAEREQVALEKAREKAEQEKAEREKAEREKAERERVEREKAREKLEQERIAREKAELEKAERERIAAEEARKKAELEKAELEKAERERIAAEKARKKAELEKAELEKAELEKAERERVAAEKARKKAEQEKAEQERVEREKAQEKALQEKAEQERIARKKAEQEKAERRKADLEKAERERVALEEARKKAEEEKAEQKRISRKKAELEKAKQEKAEREKADRERAKQEKAEQERISREKAEHEKAERDKAEQERVAREQAERKQAEQERVAREKAEHEKSERGKIQQERLAHERAEHQKAEREKAEHEEAKRQVFERELANRQRVARETAEREEAERERLMHEKFKAEEFERHNLAAQRIAREMAEYERVKECGPEDKYMEAEKPKPVVQSGTANPVVKEKEERTHAGQPIFNLDGSEPKHKDKMLQVCLNLKAKRLELVESAEEYGEAAKRQNEKMEELVHLRDHNLMIREQELQKIKLIDEQLEKEVRAIRRVIAEERRKIEAKQRMLDAVQAELKAEKPLDWVPFIVSSAQPNLNREQGHERGRERERRSAVGLSSQIPGAPENYGEEYPLEGHPAWGHVPEQDQYPSVDAPSWNSYPFNESKNSAPEQEQSGGPRNPLPEAPRSLGVPQKAVILPTEDYDTSQVSRLPAEFQSTIPGQTHVQNFENQPPETGFAGMFCMNCHWVFEKLRCSCTWGSLSRARPLLCQWCGWEHGFLYCPTHRLSFWDHTSVPVQPPAELTRVSHPTSSYQSGYQGQTYVPQPQWNAPPPQWNAPPPHLNAPQQGLHEFQPQFNPSQGPLMSPREPVWVHNDAIPEPNLQGPYTQPNHHRQLYDPRNPSRSQNEPYTQPNHHRQLYDPRNPSRSQNEPYPSGSNPPKGSRAERPSRPRRTSSSHSGPNQPGLGPRLWSPNQKKLLDSFKGDGDRGEQVPSQAPVAEGNIVTSSTLPSVQEETKSAGRGRQRKHRNSR

Target ORF information:

RefSeq Version	XM_958899.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A hypothetical protein (NCU02858), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_958899.3

ORF Insert Sequence:

ATGGTTCCGG AGGGCCCTAC TGCTCCCGAA ACGCTTACCG GCTTTAGCCC AGCTCAGCTT 
CATCCTGAAA GCTTCCCGCC CTTATATGTC AATCCAGACA GAACTCCAGT TTCCGGCACG 
CCTGGTGGTG TAGCGCCATC GTCTGCTGGC GAGGGACAGT CGGTTGAACC AGAAAAGCAC 
GAACCCGCTG TCCCGGAATT TGCGCAAGAG AATCCAGCGA ATGACAACAA CGAAGACAAC 
GGTCTGGGAG ATGGTGAACG GCAGAATATC GACGCCTGGG CAAGAGGAGA AGCTGAACTC 
ACAACCCATG TTGAGCCCGA TGATGAAGCA CCTGCAGCCG GGTCCCCGGA TATTCCCGAG 
TTATGTAATG TTCTACGTCC ACCTCGTTCA TTGAACTCCT CGGACAATAA TTCGTCGGAT 
ACATCAGAGG TTGCTGCTAA AGGCCAGTTT CAACACGAAT CCCTGGACGC GTGGCAAAGA 
CGGTTACAAG ACGAGTCCCG GGGATTTCCA GAGCCCCCTA TGGGTACGAT GGCCTCTATG 
TTTGATTATA CTCCTTTTTC CGAGACTGCT TCCAACGCAC CTGCGTCCAA AGACACTCCG 
CCCAACGATT TGCCCGGCGA TAGAATCGCA GAACCGACGG ATGCTGCCTC ATCTTCGGAC 
GGCACTCTCT TCGATGCCGA GACCACCCCG CTGGCCACCC CGCCGCACAA TGGTAACGGC 
GTTATTCCCA CATTAATTAG CTCATTTGAC AAACGCACGT TCTTTGAAAA CCTGTCGAGA 
GTTTTCAAAA AGGGAAGAAA ACGAGCAGAG AAACTTCGTG AGCGGATCAC GTTGCTTGAA 
ACTGGGATTG GCGGTGCTGT TCCAACGTCA ATTGATGAAC AGAAGAACAA AGACCACGTC 
CAACACATGC CTGAAGAGAG AAGCCGCTAT AACAGTATCA TGATCAAATT ATTAAAAGAA 
GGATACAATG TTCCAATACC GTGGGATGCA CTCGAAGAGC CGCAAAATGA AGAAGAAGAT 
ATCAGCAGCT CCGCGGGACC TCTCATGAAG CAAGCACAAG CGGGAGTCTC TCCCCCGCAA 
AACATCTCTG AAGCTGGCAA AGAGGAATCA CTTGAAGCTG GAAGAGCCTT GGACAAATTA 
CTCGATGAAA AACTGGGAGA AGCCAAAAAC GCTCAGAAAA AGGTAGCTAA AGGGGAAACG 
AAGACCGATG GCCCCCAAGT TAACCCGATT GAGCACCAGA AGCCAAATGT TGCTAAGACA 
CCGGATAACC CCGAGAAGCC AGAGGTCCTC GAGGCAAGGG GAGAACAAGC TCCGGAGTAC 
AAGACCCTCG CAGAAGAAAG GCGTAAAGCC GAGCGGGAAG ATGCGGACCG CAAGCCAGCG 
GAGCAAACTG TCAAGGGTGA GGAAGAGGAA ACCGCAAAGA ATGACGGGGA TGTCAAGCCC 
AAAACATTGG AGGAGCAGCA AAAAGCTAAG CAAGAAGAAG AGGATCGCAA ACTAGCACAG 
CAGATTGCCA AAACCCAACA AGAAGAAGCC GCAAAAGCTT CCGAGGATGC AAAGCGCAGA 
CAGGCAGAGG AAGCTGACGA TCTAGCCAAG TCCACCGAGG CCGAAAAGGA CAAAGCCCTC 
GGAGAGCGGA TCAAGGCTGC TGAAGCTGAA ATGCTCAAGG AAATTGGACA CGCAAGGGCT 
AAAGTTGCAG AGGCCGAGAG GCTCACAAAG ACCGAAAAAA ACAAGGTCAA AACGGCCAAT 
TCTGAAATGT CCGTAGCCCG CATAGATGCC GAGGCTCTTG AAGAAAAACG ACGCAAAGCC 
GCGCAAGAGG GCAAAGCCAA AGACGCTGAA GCGCTTGAAG ACAGGAGGCG CAAAGCACTT 
GAATTGGCCG AAAAAGCTGC GGTTGCAGAA AAAGAAGCGT CTGAAAAGGT CAAGGTGTAT 
CAAGAGGCGG AAGACAAAGC CCGTGAAGAG CTCAGACTCC TTGAGCTTGT GAGGCAGGAC 
GCCGTCAAAG CGGAGAAGGC CAAGGCTGCT GAACTTGGGA ATTGGAACGT GGCCGTGAAC 
GAAAAGGCAG CTGCAGCCGA AAGAAGAAGG ATCGTTCGCG AGGCAGAAGA GGCTACTTTG 
GCTAAAACCA CCGTCGAGAA AACCCCCAAG ACTTTCACTC CAGGAGGAAA ACTCACCTCT 
GTTGCTGCAT CGAGAGGTGA TGTGTCGAGG ACAAGAGACA ATCACAAATT TGTGCCATTT 
TTGAAGACTT CGAAAGTTGC TGCAGCAAGA AGTGAAGCCG CCAAAGATGC CGATCGCAAG 
CACGCAGCGG CCGGAGCTGA GAAGCGCCAG GCACTCGAGG CTGCTGGGGC TGAAAAGCGC 
AAAGAGATCA AGAAGGGGAA TCAGCAGCCG GGCGTTGTGA AAGCTTCCGA AGCGAACGTT 
CCCAAGTCTG CTGATCCGGC CATTTCCAAA TCTCCTGAAG CGAGTATTCC CACAGCTCTG 
ACAGAAGCAG AGAAACCAAG AAACTTTTAC GAGACCATAA GGAGGCGCAG GGAATGGGAA 
GCTCAACGGG AGGAGGCTAA GAAAAAAGAC GAGGCTGAAA AAATCATTAA ACCCCCTGAA 
GCCGATATTT CCAAGCCTTT CGAAGCGAAC GTTCCCAAGC CTGCTGATCC GGTCATTTCC 
AAATCCCCTG AAGCGAGTAT TCCCACGCCT CTAACAGAAG TAGAGAAACC AAGAAACTTT 
TACGAGACCA TAAGGAGGCG CAGGGAATGG GAAGCTCAAC GGGAGGAGGC TAAGAAAACA 
GACGAGGCTG AAAAAATCAT TAAACCCCCT AAAGCCGACA TTTCCAAGCC TTCCGAAGCG 
AACGTTCACA AGGCTCCTGA AAAAATCAAT AAACCCCCTG AAGCCGATAT TTCCAAGCCT 
TCCGAAGCGA ACGTTCACAA GCCTGCTGAT CCGGCCATTT CCAAATCTCC TGAAGCGAGT 
ATTCCCACAC CTCTAACAGA AGCAGAGAAA CAAAGAAACT TTTACGAGGC CATAAGGAGG 
CGCAGGGAAT GGGAAGCTAA ACGGGAGGAG GCTAAGAAAA AAGATGAGGA AACACGACGG 
GACTGGTTGG AGGAGAAAAA GAAGCAAATG CATGCCAACG ACAAACCTGC TTCTACTCAG 
CAGGCGAAGG TGGAAGAGGC ATGGCCCAGT TTGCCCTCTA CATCACAGCA GGTGAAGCCT 
GAAGCTCTGG GTGCTGCCCC TCCATCAGTC AAACTTCCCA AAATTATCAA GATTGCCCCG 
CCTAAAGAGA AGTCTCAAAG CTCCGCTAAG GCAAACCCGC AGCAGAAGCC TCAATCGCCT 
ACCCGAAAGC CGCAGCAAGC CTGGTCTCAA CCGAAGCCAG ATGGCAATGA TCGCGGAAAG 
ACCCAGACTC TGGTACAGGT GAAATCGCAA GAAAAAACTC AAGAGCAGGC TCAAGCTTCG 
GCTGCGGAAA ACACTCAGGG TCAATCTCAG AAGACCATGA AAGGCCCGGC GCCAAAGACG 
CATCCCGTTC CAGCTAAGGA GAAGCCACAG CCTCCAGCTC AGAAGAAGCC ACAGGATCCA 
GTTAAGAAGC AACCACAGTC CCAAGCACAG AAGAAGTCAC AGCAACCACC TCAACGAAAG 
GATGAAGCTC CAGCTCAGCA AAAGCCTCAA GTTCCAGCCG AGGAGAAACC CCAGCCTTCA 
CCTCAGAAGA AGCCACAGCA ACCACCCCAA CAGAAGAGTC AAGCTCCTGC CCGAGTAGAA 
AAACCGGAAC CTTCTGCCTG GAAGAAAAGG ATTAACAATT TGAATGCTAA AATCTCTGAA 
GAAAGTACTC ATACAAAGTC TCAGCCGCCT CGACCCTCAA TTGTATTTGG GGAGAAAAAG 
AACGAATTGC AGAAGGGAAA CAGCGCAGAT CAGACCCAGG CTGAAGTTCC AACTGACAAT 
AAGCCTCAAC TTGAGCCTAC ATCAAGCAAA ACAAAGCAAG ATGTTGGACC TGAAGCCACT 
CCAGCTACAA AAATGGACGA CAAAAAGGTC GAGGAGACAC TATACGTTCC ACCACAGCAT 
CAAGAAACGG TTGATACTAA GGCGCAAGAA GACCAGCTGA AGGCCCAGAA AGTCGAGACT 
GAAAGACTTG AGCACGAGAA AGCCGAGCAA GAACGCGTTG CACGAGAGAA GGCAGAAAGG 
GCGGAGCGCG AAAAGGCTGA GCGTGAAAAA GCTGAACGTG AGCAGGTCGC ACTCGAGAAA 
GCGCGCGAGA AAGCCGAACA AGAAAAAGCT GAGCGTGAAA AAGCCGAGCG AGAAAAGGCC 
GAACGGGAGC GTGTTGAACG TGAGAAGGCG CGCGAGAAGC TTGAGCAGGA GCGCATCGCA 
CGTGAAAAGG CTGAACTCGA GAAAGCTGAA CGTGAGCGGA TTGCAGCTGA GGAGGCGCGC 
AAGAAAGCTG AACTTGAGAA AGCTGAACTT GAGAAAGCTG AACGTGAGCG GATCGCAGCT 
GAGAAGGCGC GCAAGAAAGC TGAACTTGAG AAAGCTGAAC TTGAGAAAGC TGAACTTGAG 
AAAGCTGAAC GTGAGCGGGT CGCAGCTGAG AAGGCGCGCA AGAAAGCTGA GCAAGAAAAA 
GCCGAACAGG AGCGAGTTGA ACGTGAGAAG GCGCAGGAGA AAGCCCTACA AGAAAAGGCT 
GAGCAGGAGC GCATCGCACG CAAAAAGGCT GAACAGGAAA AGGCTGAGCG CCGAAAGGCC 
GATCTCGAGA AAGCTGAACG TGAGCGGGTC GCCCTTGAGG AGGCGCGCAA AAAGGCGGAA 
GAAGAAAAGG CTGAGCAGAA ACGCATTTCA CGCAAGAAGG CTGAGCTTGA GAAGGCCAAA 
CAGGAAAAGG CTGAGCGCGA AAAGGCTGAT CGTGAGAGAG CTAAGCAAGA AAAGGCTGAG 
CAGGAGCGTA TATCACGAGA GAAGGCTGAA CATGAGAAAG CTGAGCGTGA CAAGGCCGAG 
CAGGAGCGCG TTGCCCGTGA ACAGGCCGAA CGTAAGCAGG CCGAGCAGGA GCGGGTCGCA 
CGCGAGAAAG CCGAACATGA AAAGAGTGAG CGCGGAAAGA TCCAACAGGA ACGGCTTGCA 
CACGAGAGGG CTGAACACCA GAAAGCCGAG CGCGAGAAAG CCGAGCATGA GGAAGCTAAG 
CGCCAGGTAT TCGAACGTGA ATTAGCCAAT CGTCAAAGGG TTGCTCGCGA GACGGCAGAG 
CGTGAAGAAG CCGAACGCGA GAGATTGATG CACGAAAAAT TCAAGGCTGA AGAATTCGAG 
CGGCACAACC TCGCGGCCCA AAGGATCGCA CGTGAAATGG CCGAATATGA AAGGGTTAAA 
GAATGTGGAC CCGAGGATAA GTATATGGAA GCTGAAAAAC CCAAGCCAGT TGTCCAGAGT 
GGAACTGCAA ACCCGGTGGT CAAGGAAAAG GAGGAGAGAA CCCATGCTGG CCAGCCAATT 
TTCAATCTTG ATGGCTCGGA ACCAAAGCAT AAGGATAAAA TGCTCCAAGT TTGCTTGAAC 
TTGAAAGCAA AGAGGCTTGA ATTGGTTGAA AGCGCCGAGG AGTATGGTGA AGCAGCGAAG 
CGCCAGAACG AGAAGATGGA GGAACTTGTC CATCTGAGGG ATCACAATCT CATGATCAGA 
GAGCAAGAGC TTCAGAAGAT AAAGCTAATT GATGAACAAC TGGAGAAGGA AGTGCGCGCT 
ATACGTAGAG TAATCGCAGA AGAGCGACGA AAGATAGAAG CGAAGCAGCG GATGCTTGAT 
GCGGTGCAGG CGGAATTGAA GGCTGAAAAA CCTCTTGACT GGGTTCCATT CATTGTGTCC 
TCGGCCCAAC CCAACCTGAA CCGGGAGCAA GGACATGAAA GGGGGAGGGA AAGGGAGCGC 
CGCTCTGCGG TGGGCTTGAG CAGTCAAATT CCAGGAGCAC CTGAGAATTA TGGCGAGGAA 
TACCCGCTGG AAGGACATCC CGCTTGGGGA CATGTACCCG AGCAGGACCA GTATCCTTCA 
GTTGACGCTC CGTCTTGGAA TTCGTATCCT TTTAACGAAT CCAAAAATTC AGCACCAGAA 
CAGGAGCAGT CAGGAGGACC TAGAAACCCG CTACCCGAAG CACCAAGGTC ACTTGGAGTC 
CCCCAGAAAG CTGTGATTCT TCCTACAGAA GACTATGATA CATCTCAGGT ATCAAGACTA 
CCCGCAGAGT TTCAAAGCAC GATCCCAGGC CAGACTCACG TGCAGAACTT CGAAAACCAG 
CCCCCAGAAA CCGGCTTCGC GGGCATGTTC TGCATGAATT GCCACTGGGT ATTCGAAAAG 
CTGCGTTGTT CCTGCACCTG GGGCTCACTC AGCCGAGCTC GTCCCCTTCT CTGCCAATGG 
TGCGGATGGG AACACGGCTT CCTCTATTGT CCCACTCATC GTCTAAGCTT TTGGGACCAT 
ACCTCTGTGC CTGTTCAACC TCCTGCTGAA CTGACTCGGG TCTCGCATCC AACATCATCA 
TATCAAAGTG GCTACCAAGG ACAAACATAC GTGCCCCAAC CGCAGTGGAA TGCGCCTCCA 
CCGCAGTGGA ATGCGCCTCC ACCGCATTTA AACGCACCAC AGCAAGGCTT GCATGAATTC 
CAACCACAAT TCAACCCATC CCAAGGACCT TTGATGTCGC CACGGGAACC TGTATGGGTG 
CACAATGATG CAATTCCTGA ACCAAATCTA CAAGGGCCGT ATACTCAACC AAACCATCAT 
CGACAACTTT ACGACCCTCG CAACCCAAGC AGGTCACAAA ATGAGCCGTA TACTCAACCA 
AACCATCATC GACAACTTTA CGACCCTCGC AACCCAAGCA GGTCACAAAA TGAGCCGTAT 
CCGTCAGGTT CTAATCCCCC AAAGGGTTCA AGGGCCGAGA GGCCTTCGAG GCCCAGAAGG 
ACGAGCAGTT CACACAGTGG CCCGAATCAG CCAGGGTTGG GGCCACGCCT TTGGAGTCCA 
AACCAAAAGA AGTTGTTGGA CTCGTTCAAG GGAGATGGTG ATCGTGGAGA ACAAGTCCCT 
TCGCAGGCTC CTGTTGCGGA AGGGAATATC GTAACAAGCT CGACGCTTCC GAGTGTCCAG 
GAGGAGACCA AGTCCGCAGG AAGAGGTCGT CAAAGAAAAC ACCGCAACTC AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU02858 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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