crf6-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	crf6-1
Entrez Gene ID	3880017
Full Name	chromatin remodeling factor 6-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011394764.1	XP_011393066.1	chromodomain helicase, variant
XM_011394765.1	XP_011393067.1	chromodomain helicase

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_04589	XP_003713613.1
Neurospora crassa	NCU03060	XP_963868.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the Neurospora homologue of chd1 (NCU03060) is required for normal remodeling of chromatin at frq and is required for normal frq expression and sustained rhythmicity.
Title: CHD1 remodels chromatin and influences transient DNA methylation at the clock gene frequency.

The following crf6-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crf6-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf02341	XM_011394765.1 Latest version!	Neurospora crassa OR74A chromodomain helicase (crf6-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ONf02341	XM_011394764.1 Latest version!	Neurospora crassa OR74A chromodomain helicase, variant (crf6-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crf6-1 ( XM_011394764.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393066.1 Neurospora crassa OR74A chromodomain helicase, variant (crf6-1), mRNA.

Price & Availability↑

CloneID	ONf02341
Clone ID Related Accession (Same CDS sequence)	XM_011394764.1 , XM_011394765.1
Accession Version	XM_011394764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	chromodomain helicase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGACAT CTCCCGAATC CGGTCCGGTG TTCGGGCATG TGTCACCGCC GGCGGATGAC 
AGCCACTTCA ACTTTACCAA CGATACCGAA AGCACGAGCG ACCTTTCAGA CGTTAATGCC 
GCCGAGGCCG ACCCAAATGA CGACGCCGAC GCCCAGTCGT ATCAGCCTGC CGACGAGGCT 
GTCTCGCCGA TACAAAAACC CGAGTTGACG TTCGAGGAAG CGTCCGATTC AGCAAGTGAT 
AACGACGCGT CAGATGACGG AGACTTTGAC GCCCCCGATA GCCCCAGGTC TGCTCAGAGC 
AACGAAGTCG TGTCTGCCTC GTCGTCCCGT ACCGTCGTGA AGCGCAAAGT CGCCCACGCG 
ACCGAGGATG ATTACATCAG AGAGAATCCT GCCCTGTATG GGTTGAGGAG GAGTTCACGC 
CCTACACAAC GCCGTGCGCA GATCATCGAC TCAGATGAAG AAGATGAACC ATCCGAATCG 
GATGCCACAC CGGTGTCTAG AAAGCCTGCG AAGAGACGTC GGGTTGAACG CTCGTTGCCT 
GCTTCCAAGC GTGGCACTCC TGCTCGCCAA CCCTCAATCG ATGATTCCGA CTCGGACACA 
TATGGAGGCG CCAAAGCCCG CAGCTTCCAA AGAAAGGTCC GCAGGCAACA AGAACAGGAC 
CCTGCCTTGG CTTTTAGCGA AAAGAGATGG TCTAGCCGTC GGGCCGCCCA GGTCTCAGCC 
GGTGCCTACC AAGAAAGTGA CGCCGAAGAA GATGAAGAGG ACGACTCCGA GCTTACCCCC 
AACTACTGGT CTGCCAATGT CGAGGATAAC TCGCCATACA TCGAGAAAAT CCTCGGTCAT 
CGTCCTAAGG AGGGTGTCGA GGTCACGGTG GATTCTACGC GACACGACTT TGAATACTTC 
ATCAAGTGGC AGGGACAATC ATATCCCCAC AACACCTGGG AAACTACGGA AACTGTCGCA 
GGCTTCCGAG GCTTTCGCCG CCTCGAAAAC TATTACAAGA AGGTTGTTGA GTACGAACTG 
GAGATGCAAT TCGGTGGCGA CGATATCAGT CCGGAGCAGA AAGAGCAGTG GTACCTGGAT 
AGAGAACGTG AGCAAGAGGC CCTCGAAGAC TACACAAAGG TCGAGCGCGT TGTGGCTGTC 
AGAGACGGTG ATGAGGGCAC CGAGTATTAC ATCAAGTGGA AGGGCCTGCA ATACGACGAG 
TGCACCTGGG AAGCCGAGTC TCTCGTGGCC GAACTTGCTC AGGACAAGAT TAACCAGTTC 
CTCGACCGAT GCAACCGGTC ATGGCAGTCT GACCGCAAGC AAACTAACCC CGATACCCGG 
TCGAGGATGA CCAAACTGGA GAAGCAGCCT GACTATATCA AGGGCGGCGA GCTTCGCGAG 
TTCCAAATGA AAGGCCTTAA CTTCCTTGCT CTCAACTGGG TAAGGGGCAA CAACGTCATC 
TTAGCCGATG AAATGGGTCT GGGAAAGACT GTCCAAAGCG TCTCGTTCCT CAGCTGGTTA 
CGGAATGAAC GCGGCCAGGA AGGACCGTTC CTTGTCGTCG CTCCTCTTAG CGTCATCCCC 
GCTTGGTGCG ATACATTTAA TCATTGGGCT CCGGATCTCA ATTACGTCGT TTACCTGGGC 
CCGGAGGCAG CTCGGTCCAA CATTCGAGAT TACGAACTTT TTGTGGATGG TAACCCAAAG 
AAGACCAAGT TCAATGTCTT AGTCACTTCC TATGACTACA TCTTGGCCGA TGCGGATCAT 
CTCAAGAACA TCAAATGGCA AGTTCTCGCT GTTGACGAGG CCCACCGTCT CAAGAACCGA 
GAGTCACAGC TCTACATCAA GCTCAACAAC TTCGGCGTTC CTTGCAAGGT CCTCATTACC 
GGCACACCCA TTCAGAACAA TCTGGCCGAA CTCTCAGCCC TCCTCGACTT CCTTAACCCC 
GGAAAGGTCG TCATTGATGA GGAGCTTGAA GCGTTGTCTA CCGCAGATAA CAAAGGTCCC 
ACTGATGAAG AACAGGATGA GGCTAGGCGT ATCAGGACCC AAGAGAAGCT CCGCGAGTTG 
CATCAGTCAA TCGCTCCTTT CATTTTGCGT CGCACCAAGG AGACGGTCGA GTCGGACCTC 
CCCCCCAAAA CCGAAAAGAT CATCCGAGTT GAGTTGTCTG ACGTGCAACT TGACTACTAC 
AAGAACATTC TCACACGCAA CTATGCTGCC CTCAGCGACG CGAGCAACGG CCACAAGCAA 
TCGTTGCTCA ACATCATGAT GGAGCTGAAG AAAGTCAGTA ACCACCCGTA CATGTTCCAG 
GGTGCCGAGG AGCGTGTCCT CAACGGCAGC ACACGCCGTG AAGACCAGAT CAAGGGTCTC 
ATTACAAGTA GCGGCAAGAT GATGCTTCTT GATCAGCTGC TGGCAAAACT TAGGAAGGAC 
GGACATCGTG TTCTGATCTT CAGTCAGATG GTCAAGATGC TAGACATTTT GGGTGACTAT 
CTCCGTGTTC GCGGATATCA ATTCCAGCGA CTCGACGGTA CCATTCCTGC CGGCCCAAGA 
CGTATGGCGA TCAATCATTT TAACGCCGAG GGCAGCGATG ATTTCTGCTT CCTTTTGTCT 
ACCCGCGCCG GTGGCCTTGG AATCAATCTT ATGACGGCTG ATACTGTCAT CATCTATGAC 
TCGGACTGGA ATCCCCAGGC CGATCTTCAG GCAATGGCTC GAGCTCACCG TATCGGCCAG 
AAAAGACCTG TTAATGTCTA CCGCCTCGTG GCCAAGCAGA CTATTGAAGA GGAAGTTGTA 
AAGCGAGCCC GCAACAAGCT CTTCTTGGAG TATCTTACCA TTCAGGCCGG TGTGACAGAT 
GAAGGCAAGG CTCTCAAAGA GCAGTTCAAG GAGCGCGGCC TCAAGATGGA CGAGGCTAAG 
ACCGCTGAGG ATATCTCGAT GATCCTCAAG ATGCGTTCCC AGAACCTGTT CGAACAATCT 
AGCAACCAGC AGAAGCTGGA GCAGCTCGAT ATCGATGCTA TCCTCGAGAA TGCCGAAGTT 
ACCAAAACTG ATGTCGATGA CAAGATCAAC CTGAGCAGTG GTGGTATCGA CTGGGATAAC 
TGGATGCAGG TCACCGATGT CAAAGTTGAC GATCTCGCCC TTGACTGGGA TCAGATCATT 
CCAGCCGAGC AGCTTGCTGC CATCAAGGCT GAGGAAAATA GAAAGAAGGA AGAGGAGTAC 
TTGGCAAAAA CGATAGAGGA GAATGCTCCT AGAAAAGCTG CGTTGAAAGG CTCCAAAAAG 
AGCGCAGAGA ACGATCGAGC TGAGCGTCTG GCAAAGAAGC GACAACGCGA GAGGGAAGAG 
CAGGAAGAGC TCGAAGAGCA ACGTGCATTG CTTTCTGATC CCAAACGCCC CCTCAATGAG 
AAAGAAACCC GAAACCTTAT CAGGGCCTTC TTCAGGTACG GTTCCTTGGA CGACCGCGAA 
GAAGAGGTTG TCCAGGATGC CCGCCTTAGT GATAGAGATC GGGACTTTCT GAAGTCTATT 
ATCGAGGACC TTGTCGTTAT CAGTCGCCGC GCTGTAGATG CGAACAACGA GAGACTTCGC 
GAAGAAGAAG CCAGGGCTGG CAAGCCACTG GCGAAGAAGG ATAAGAAGGC TGTACTTGTC 
GATTTTGGCG AGGTGCGGAA AGTCAACGCA GAGACTGTCG TGGAGCGACC CCCTCAGCTC 
AAACTCCTGC GTCGCGTCCT CCGGGAGCAC GCAGACATTC TCAGTTTCAG GCTTCCCGAA 
GCTTCCAAGG CTGCCGCATA TACTTGCGAC TGGGGTGCAC GCGAGGATGC CATGCTGCTT 
ATTGGAATCG ACAGGTACGG ATTTGGTGCG TGGACGCAAA TCAGGGACGA TCCTGAGTTG 
CAGATGCACG ACAAGTTCTT CCTGGAGGAG CATCGTGTCG ACAAACGGGA AGAACGAAAG 
AAGACGGAGG CTGTAGGGAT TCAATCCCCT GGTGCTGTTC ATTTGGTCCG AAGGTCCGAG 
TATCTCCTGT CTGTTCTGTT GGCCAAGCAC TCTAATGATG CTGCCGCTAA GAAGGCAGTG 
GAGAACCACC ATCGGAGCAA GAAGCTTCTC ACGAACAACG GCCATCGACG CGGTGAAGCT 
AGCTCCATCT CCGGGTCTCC TGCACCATTG GGCGTTAAAA AGTCTATTCC TCACCGGGAC 
CACCACCATC GGACTCTCAG CAACGCGAAT GACCGGGGGA CCCCCAGGCC TGACCACAAA 
CGGAAGTACA CCGATGATTA CGAAGAGCGC AGCTCGAAGC ATCGTCGGGT GGAAGACGAA 
CGTCGTGGTA CAGATAGCAA ACTCGACAAG AAGAAGGACA AGGCACCCAA GGTCGATCCC 
GAAACATTGG AGAAATGGAA GCGGGACAGG CAGAAAGCGG TGGAGCGCTT CCATGAACTT 
GCCAAGTTGA ATAATGATGA GATCGATAGC TCGGACAACT TCCAACTTGT TTGGTCTCTA 
CTTCGGCCCC TCAAAGCGAA CATGGAACGC ATCATGTACT CTAAGGAACG TTTTCCTGCG 
GCCAAAGAAC GCGCCAAAAT TTTGGGAGAG GAGATTCGGG CATTTGGTAA TTTCCTTAAG 
CAGCTGGAGG AGAAGAACCC GCTGGATGGT TTGGAGCCAC AGTTCTGGGA ATTCCTTTCT 
TCGATCTGGC CCATCGGTGA GGTCAAGGTG TCGGGCCGAA GACTCCAGCG CATGTTCGGC 
GACCTTTGCA AAAAGGATAG CGAGAACAAG GAGGACAGAT CTCAGAATCT GAAGAAGCCG 
AGCCGCCAGG ATCTCGAAGA TGGCGAGATT GCCAGCGATC GCGACGATCG AAGATCTCGT 
GACCCTTACC GCGACGACCG GCGCGAGGAC CGGCGCGAAG AGCGACGAGA GGAGCCCAAG 
CGGCGCCCGA ACTATTACGA GAATGGCGAT CGACTCAACT TTTATCGCCA TCGTCGGGAC 
ATCGATAGGG GAGCACCGAT CCGTCACTCA TGGCAGAGCA GCCGCAGTCC CTCTCAGCAG 
CAACGGCCTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393066.1
CDS	197..5191
Translation	MSTSPESGPVFGHVSPPADDSHFNFTNDTESTSDLSDVNAAEADPNDDADAQSYQPADEAVSPIQKPELTFEEASDSASDNDASDDGDFDAPDSPRSAQSNEVVSASSSRTVVKRKVAHATEDDYIRENPALYGLRRSSRPTQRRAQIIDSDEEDEPSESDATPVSRKPAKRRRVERSLPASKRGTPARQPSIDDSDSDTYGGAKARSFQRKVRRQQEQDPALAFSEKRWSSRRAAQVSAGAYQESDAEEDEEDDSELTPNYWSANVEDNSPYIEKILGHRPKEGVEVTVDSTRHDFEYFIKWQGQSYPHNTWETTETVAGFRGFRRLENYYKKVVEYELEMQFGGDDISPEQKEQWYLDREREQEALEDYTKVERVVAVRDGDEGTEYYIKWKGLQYDECTWEAESLVAELAQDKINQFLDRCNRSWQSDRKQTNPDTRSRMTKLEKQPDYIKGGELREFQMKGLNFLALNWVRGNNVILADEMGLGKTVQSVSFLSWLRNERGQEGPFLVVAPLSVIPAWCDTFNHWAPDLNYVVYLGPEAARSNIRDYELFVDGNPKKTKFNVLVTSYDYILADADHLKNIKWQVLAVDEAHRLKNRESQLYIKLNNFGVPCKVLITGTPIQNNLAELSALLDFLNPGKVVIDEELEALSTADNKGPTDEEQDEARRIRTQEKLRELHQSIAPFILRRTKETVESDLPPKTEKIIRVELSDVQLDYYKNILTRNYAALSDASNGHKQSLLNIMMELKKVSNHPYMFQGAEERVLNGSTRREDQIKGLITSSGKMMLLDQLLAKLRKDGHRVLIFSQMVKMLDILGDYLRVRGYQFQRLDGTIPAGPRRMAINHFNAEGSDDFCFLLSTRAGGLGINLMTADTVIIYDSDWNPQADLQAMARAHRIGQKRPVNVYRLVAKQTIEEEVVKRARNKLFLEYLTIQAGVTDEGKALKEQFKERGLKMDEAKTAEDISMILKMRSQNLFEQSSNQQKLEQLDIDAILENAEVTKTDVDDKINLSSGGIDWDNWMQVTDVKVDDLALDWDQIIPAEQLAAIKAEENRKKEEEYLAKTIEENAPRKAALKGSKKSAENDRAERLAKKRQREREEQEELEEQRALLSDPKRPLNEKETRNLIRAFFRYGSLDDREEEVVQDARLSDRDRDFLKSIIEDLVVISRRAVDANNERLREEEARAGKPLAKKDKKAVLVDFGEVRKVNAETVVERPPQLKLLRRVLREHADILSFRLPEASKAAAYTCDWGAREDAMLLIGIDRYGFGAWTQIRDDPELQMHDKFFLEEHRVDKREERKKTEAVGIQSPGAVHLVRRSEYLLSVLLAKHSNDAAAKKAVENHHRSKKLLTNNGHRRGEASSISGSPAPLGVKKSIPHRDHHHRTLSNANDRGTPRPDHKRKYTDDYEERSSKHRRVEDERRGTDSKLDKKKDKAPKVDPETLEKWKRDRQKAVERFHELAKLNNDEIDSSDNFQLVWSLLRPLKANMERIMYSKERFPAAKERAKILGEEIRAFGNFLKQLEEKNPLDGLEPQFWEFLSSIWPIGEVKVSGRRLQRMFGDLCKKDSENKEDRSQNLKKPSRQDLEDGEIASDRDDRRSRDPYRDDRREDRREERREEPKRRPNYYENGDRLNFYRHRRDIDRGAPIRHSWQSSRSPSQQQRPY

Target ORF information:

RefSeq Version	XM_011394764.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A chromodomain helicase, variant (crf6-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394764.1

ORF Insert Sequence:

ATGTCGACAT CTCCCGAATC CGGTCCGGTG TTCGGGCATG TGTCACCGCC GGCGGATGAC 
AGCCACTTCA ACTTTACCAA CGATACCGAA AGCACGAGCG ACCTTTCAGA CGTTAATGCC 
GCCGAGGCCG ACCCAAATGA CGACGCCGAC GCCCAGTCGT ATCAGCCTGC CGACGAGGCT 
GTCTCGCCGA TACAAAAACC CGAGTTGACG TTCGAGGAAG CGTCCGATTC AGCAAGTGAT 
AACGACGCGT CAGATGACGG AGACTTTGAC GCCCCCGATA GCCCCAGGTC TGCTCAGAGC 
AACGAAGTCG TGTCTGCCTC GTCGTCCCGT ACCGTCGTGA AGCGCAAAGT CGCCCACGCG 
ACCGAGGATG ATTACATCAG AGAGAATCCT GCCCTGTATG GGTTGAGGAG GAGTTCACGC 
CCTACACAAC GCCGTGCGCA GATCATCGAC TCAGATGAAG AAGATGAACC ATCCGAATCG 
GATGCCACAC CGGTGTCTAG AAAGCCTGCG AAGAGACGTC GGGTTGAACG CTCGTTGCCT 
GCTTCCAAGC GTGGCACTCC TGCTCGCCAA CCCTCAATCG ATGATTCCGA CTCGGACACA 
TATGGAGGCG CCAAAGCCCG CAGCTTCCAA AGAAAGGTCC GCAGGCAACA AGAACAGGAC 
CCTGCCTTGG CTTTTAGCGA AAAGAGATGG TCTAGCCGTC GGGCCGCCCA GGTCTCAGCC 
GGTGCCTACC AAGAAAGTGA CGCCGAAGAA GATGAAGAGG ACGACTCCGA GCTTACCCCC 
AACTACTGGT CTGCCAATGT CGAGGATAAC TCGCCATACA TCGAGAAAAT CCTCGGTCAT 
CGTCCTAAGG AGGGTGTCGA GGTCACGGTG GATTCTACGC GACACGACTT TGAATACTTC 
ATCAAGTGGC AGGGACAATC ATATCCCCAC AACACCTGGG AAACTACGGA AACTGTCGCA 
GGCTTCCGAG GCTTTCGCCG CCTCGAAAAC TATTACAAGA AGGTTGTTGA GTACGAACTG 
GAGATGCAAT TCGGTGGCGA CGATATCAGT CCGGAGCAGA AAGAGCAGTG GTACCTGGAT 
AGAGAACGTG AGCAAGAGGC CCTCGAAGAC TACACAAAGG TCGAGCGCGT TGTGGCTGTC 
AGAGACGGTG ATGAGGGCAC CGAGTATTAC ATCAAGTGGA AGGGCCTGCA ATACGACGAG 
TGCACCTGGG AAGCCGAGTC TCTCGTGGCC GAACTTGCTC AGGACAAGAT TAACCAGTTC 
CTCGACCGAT GCAACCGGTC ATGGCAGTCT GACCGCAAGC AAACTAACCC CGATACCCGG 
TCGAGGATGA CCAAACTGGA GAAGCAGCCT GACTATATCA AGGGCGGCGA GCTTCGCGAG 
TTCCAAATGA AAGGCCTTAA CTTCCTTGCT CTCAACTGGG TAAGGGGCAA CAACGTCATC 
TTAGCCGATG AAATGGGTCT GGGAAAGACT GTCCAAAGCG TCTCGTTCCT CAGCTGGTTA 
CGGAATGAAC GCGGCCAGGA AGGACCGTTC CTTGTCGTCG CTCCTCTTAG CGTCATCCCC 
GCTTGGTGCG ATACATTTAA TCATTGGGCT CCGGATCTCA ATTACGTCGT TTACCTGGGC 
CCGGAGGCAG CTCGGTCCAA CATTCGAGAT TACGAACTTT TTGTGGATGG TAACCCAAAG 
AAGACCAAGT TCAATGTCTT AGTCACTTCC TATGACTACA TCTTGGCCGA TGCGGATCAT 
CTCAAGAACA TCAAATGGCA AGTTCTCGCT GTTGACGAGG CCCACCGTCT CAAGAACCGA 
GAGTCACAGC TCTACATCAA GCTCAACAAC TTCGGCGTTC CTTGCAAGGT CCTCATTACC 
GGCACACCCA TTCAGAACAA TCTGGCCGAA CTCTCAGCCC TCCTCGACTT CCTTAACCCC 
GGAAAGGTCG TCATTGATGA GGAGCTTGAA GCGTTGTCTA CCGCAGATAA CAAAGGTCCC 
ACTGATGAAG AACAGGATGA GGCTAGGCGT ATCAGGACCC AAGAGAAGCT CCGCGAGTTG 
CATCAGTCAA TCGCTCCTTT CATTTTGCGT CGCACCAAGG AGACGGTCGA GTCGGACCTC 
CCCCCCAAAA CCGAAAAGAT CATCCGAGTT GAGTTGTCTG ACGTGCAACT TGACTACTAC 
AAGAACATTC TCACACGCAA CTATGCTGCC CTCAGCGACG CGAGCAACGG CCACAAGCAA 
TCGTTGCTCA ACATCATGAT GGAGCTGAAG AAAGTCAGTA ACCACCCGTA CATGTTCCAG 
GGTGCCGAGG AGCGTGTCCT CAACGGCAGC ACACGCCGTG AAGACCAGAT CAAGGGTCTC 
ATTACAAGTA GCGGCAAGAT GATGCTTCTT GATCAGCTGC TGGCAAAACT TAGGAAGGAC 
GGACATCGTG TTCTGATCTT CAGTCAGATG GTCAAGATGC TAGACATTTT GGGTGACTAT 
CTCCGTGTTC GCGGATATCA ATTCCAGCGA CTCGACGGTA CCATTCCTGC CGGCCCAAGA 
CGTATGGCGA TCAATCATTT TAACGCCGAG GGCAGCGATG ATTTCTGCTT CCTTTTGTCT 
ACCCGCGCCG GTGGCCTTGG AATCAATCTT ATGACGGCTG ATACTGTCAT CATCTATGAC 
TCGGACTGGA ATCCCCAGGC CGATCTTCAG GCAATGGCTC GAGCTCACCG TATCGGCCAG 
AAAAGACCTG TTAATGTCTA CCGCCTCGTG GCCAAGCAGA CTATTGAAGA GGAAGTTGTA 
AAGCGAGCCC GCAACAAGCT CTTCTTGGAG TATCTTACCA TTCAGGCCGG TGTGACAGAT 
GAAGGCAAGG CTCTCAAAGA GCAGTTCAAG GAGCGCGGCC TCAAGATGGA CGAGGCTAAG 
ACCGCTGAGG ATATCTCGAT GATCCTCAAG ATGCGTTCCC AGAACCTGTT CGAACAATCT 
AGCAACCAGC AGAAGCTGGA GCAGCTCGAT ATCGATGCTA TCCTCGAGAA TGCCGAAGTT 
ACCAAAACTG ATGTCGATGA CAAGATCAAC CTGAGCAGTG GTGGTATCGA CTGGGATAAC 
TGGATGCAGG TCACCGATGT CAAAGTTGAC GATCTCGCCC TTGACTGGGA TCAGATCATT 
CCAGCCGAGC AGCTTGCTGC CATCAAGGCT GAGGAAAATA GAAAGAAGGA AGAGGAGTAC 
TTGGCAAAAA CGATAGAGGA GAATGCTCCT AGAAAAGCTG CGTTGAAAGG CTCCAAAAAG 
AGCGCAGAGA ACGATCGAGC TGAGCGTCTG GCAAAGAAGC GACAACGCGA GAGGGAAGAG 
CAGGAAGAGC TCGAAGAGCA ACGTGCATTG CTTTCTGATC CCAAACGCCC CCTCAATGAG 
AAAGAAACCC GAAACCTTAT CAGGGCCTTC TTCAGGTACG GTTCCTTGGA CGACCGCGAA 
GAAGAGGTTG TCCAGGATGC CCGCCTTAGT GATAGAGATC GGGACTTTCT GAAGTCTATT 
ATCGAGGACC TTGTCGTTAT CAGTCGCCGC GCTGTAGATG CGAACAACGA GAGACTTCGC 
GAAGAAGAAG CCAGGGCTGG CAAGCCACTG GCGAAGAAGG ATAAGAAGGC TGTACTTGTC 
GATTTTGGCG AGGTGCGGAA AGTCAACGCA GAGACTGTCG TGGAGCGACC CCCTCAGCTC 
AAACTCCTGC GTCGCGTCCT CCGGGAGCAC GCAGACATTC TCAGTTTCAG GCTTCCCGAA 
GCTTCCAAGG CTGCCGCATA TACTTGCGAC TGGGGTGCAC GCGAGGATGC CATGCTGCTT 
ATTGGAATCG ACAGGTACGG ATTTGGTGCG TGGACGCAAA TCAGGGACGA TCCTGAGTTG 
CAGATGCACG ACAAGTTCTT CCTGGAGGAG CATCGTGTCG ACAAACGGGA AGAACGAAAG 
AAGACGGAGG CTGTAGGGAT TCAATCCCCT GGTGCTGTTC ATTTGGTCCG AAGGTCCGAG 
TATCTCCTGT CTGTTCTGTT GGCCAAGCAC TCTAATGATG CTGCCGCTAA GAAGGCAGTG 
GAGAACCACC ATCGGAGCAA GAAGCTTCTC ACGAACAACG GCCATCGACG CGGTGAAGCT 
AGCTCCATCT CCGGGTCTCC TGCACCATTG GGCGTTAAAA AGTCTATTCC TCACCGGGAC 
CACCACCATC GGACTCTCAG CAACGCGAAT GACCGGGGGA CCCCCAGGCC TGACCACAAA 
CGGAAGTACA CCGATGATTA CGAAGAGCGC AGCTCGAAGC ATCGTCGGGT GGAAGACGAA 
CGTCGTGGTA CAGATAGCAA ACTCGACAAG AAGAAGGACA AGGCACCCAA GGTCGATCCC 
GAAACATTGG AGAAATGGAA GCGGGACAGG CAGAAAGCGG TGGAGCGCTT CCATGAACTT 
GCCAAGTTGA ATAATGATGA GATCGATAGC TCGGACAACT TCCAACTTGT TTGGTCTCTA 
CTTCGGCCCC TCAAAGCGAA CATGGAACGC ATCATGTACT CTAAGGAACG TTTTCCTGCG 
GCCAAAGAAC GCGCCAAAAT TTTGGGAGAG GAGATTCGGG CATTTGGTAA TTTCCTTAAG 
CAGCTGGAGG AGAAGAACCC GCTGGATGGT TTGGAGCCAC AGTTCTGGGA ATTCCTTTCT 
TCGATCTGGC CCATCGGTGA GGTCAAGGTG TCGGGCCGAA GACTCCAGCG CATGTTCGGC 
GACCTTTGCA AAAAGGATAG CGAGAACAAG GAGGACAGAT CTCAGAATCT GAAGAAGCCG 
AGCCGCCAGG ATCTCGAAGA TGGCGAGATT GCCAGCGATC GCGACGATCG AAGATCTCGT 
GACCCTTACC GCGACGACCG GCGCGAGGAC CGGCGCGAAG AGCGACGAGA GGAGCCCAAG 
CGGCGCCCGA ACTATTACGA GAATGGCGAT CGACTCAACT TTTATCGCCA TCGTCGGGAC 
ATCGATAGGG GAGCACCGAT CCGTCACTCA TGGCAGAGCA GCCGCAGTCC CTCTCAGCAG 
CAACGGCCTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crf6-1 ( XM_011394765.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393067.1 Neurospora crassa OR74A chromodomain helicase (crf6-1), mRNA.

Price & Availability↑

CloneID	ONf02341
Clone ID Related Accession (Same CDS sequence)	XM_011394764.1 , XM_011394765.1
Accession Version	XM_011394765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	chromodomain helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGACAT CTCCCGAATC CGGTCCGGTG TTCGGGCATG TGTCACCGCC GGCGGATGAC 
AGCCACTTCA ACTTTACCAA CGATACCGAA AGCACGAGCG ACCTTTCAGA CGTTAATGCC 
GCCGAGGCCG ACCCAAATGA CGACGCCGAC GCCCAGTCGT ATCAGCCTGC CGACGAGGCT 
GTCTCGCCGA TACAAAAACC CGAGTTGACG TTCGAGGAAG CGTCCGATTC AGCAAGTGAT 
AACGACGCGT CAGATGACGG AGACTTTGAC GCCCCCGATA GCCCCAGGTC TGCTCAGAGC 
AACGAAGTCG TGTCTGCCTC GTCGTCCCGT ACCGTCGTGA AGCGCAAAGT CGCCCACGCG 
ACCGAGGATG ATTACATCAG AGAGAATCCT GCCCTGTATG GGTTGAGGAG GAGTTCACGC 
CCTACACAAC GCCGTGCGCA GATCATCGAC TCAGATGAAG AAGATGAACC ATCCGAATCG 
GATGCCACAC CGGTGTCTAG AAAGCCTGCG AAGAGACGTC GGGTTGAACG CTCGTTGCCT 
GCTTCCAAGC GTGGCACTCC TGCTCGCCAA CCCTCAATCG ATGATTCCGA CTCGGACACA 
TATGGAGGCG CCAAAGCCCG CAGCTTCCAA AGAAAGGTCC GCAGGCAACA AGAACAGGAC 
CCTGCCTTGG CTTTTAGCGA AAAGAGATGG TCTAGCCGTC GGGCCGCCCA GGTCTCAGCC 
GGTGCCTACC AAGAAAGTGA CGCCGAAGAA GATGAAGAGG ACGACTCCGA GCTTACCCCC 
AACTACTGGT CTGCCAATGT CGAGGATAAC TCGCCATACA TCGAGAAAAT CCTCGGTCAT 
CGTCCTAAGG AGGGTGTCGA GGTCACGGTG GATTCTACGC GACACGACTT TGAATACTTC 
ATCAAGTGGC AGGGACAATC ATATCCCCAC AACACCTGGG AAACTACGGA AACTGTCGCA 
GGCTTCCGAG GCTTTCGCCG CCTCGAAAAC TATTACAAGA AGGTTGTTGA GTACGAACTG 
GAGATGCAAT TCGGTGGCGA CGATATCAGT CCGGAGCAGA AAGAGCAGTG GTACCTGGAT 
AGAGAACGTG AGCAAGAGGC CCTCGAAGAC TACACAAAGG TCGAGCGCGT TGTGGCTGTC 
AGAGACGGTG ATGAGGGCAC CGAGTATTAC ATCAAGTGGA AGGGCCTGCA ATACGACGAG 
TGCACCTGGG AAGCCGAGTC TCTCGTGGCC GAACTTGCTC AGGACAAGAT TAACCAGTTC 
CTCGACCGAT GCAACCGGTC ATGGCAGTCT GACCGCAAGC AAACTAACCC CGATACCCGG 
TCGAGGATGA CCAAACTGGA GAAGCAGCCT GACTATATCA AGGGCGGCGA GCTTCGCGAG 
TTCCAAATGA AAGGCCTTAA CTTCCTTGCT CTCAACTGGG TAAGGGGCAA CAACGTCATC 
TTAGCCGATG AAATGGGTCT GGGAAAGACT GTCCAAAGCG TCTCGTTCCT CAGCTGGTTA 
CGGAATGAAC GCGGCCAGGA AGGACCGTTC CTTGTCGTCG CTCCTCTTAG CGTCATCCCC 
GCTTGGTGCG ATACATTTAA TCATTGGGCT CCGGATCTCA ATTACGTCGT TTACCTGGGC 
CCGGAGGCAG CTCGGTCCAA CATTCGAGAT TACGAACTTT TTGTGGATGG TAACCCAAAG 
AAGACCAAGT TCAATGTCTT AGTCACTTCC TATGACTACA TCTTGGCCGA TGCGGATCAT 
CTCAAGAACA TCAAATGGCA AGTTCTCGCT GTTGACGAGG CCCACCGTCT CAAGAACCGA 
GAGTCACAGC TCTACATCAA GCTCAACAAC TTCGGCGTTC CTTGCAAGGT CCTCATTACC 
GGCACACCCA TTCAGAACAA TCTGGCCGAA CTCTCAGCCC TCCTCGACTT CCTTAACCCC 
GGAAAGGTCG TCATTGATGA GGAGCTTGAA GCGTTGTCTA CCGCAGATAA CAAAGGTCCC 
ACTGATGAAG AACAGGATGA GGCTAGGCGT ATCAGGACCC AAGAGAAGCT CCGCGAGTTG 
CATCAGTCAA TCGCTCCTTT CATTTTGCGT CGCACCAAGG AGACGGTCGA GTCGGACCTC 
CCCCCCAAAA CCGAAAAGAT CATCCGAGTT GAGTTGTCTG ACGTGCAACT TGACTACTAC 
AAGAACATTC TCACACGCAA CTATGCTGCC CTCAGCGACG CGAGCAACGG CCACAAGCAA 
TCGTTGCTCA ACATCATGAT GGAGCTGAAG AAAGTCAGTA ACCACCCGTA CATGTTCCAG 
GGTGCCGAGG AGCGTGTCCT CAACGGCAGC ACACGCCGTG AAGACCAGAT CAAGGGTCTC 
ATTACAAGTA GCGGCAAGAT GATGCTTCTT GATCAGCTGC TGGCAAAACT TAGGAAGGAC 
GGACATCGTG TTCTGATCTT CAGTCAGATG GTCAAGATGC TAGACATTTT GGGTGACTAT 
CTCCGTGTTC GCGGATATCA ATTCCAGCGA CTCGACGGTA CCATTCCTGC CGGCCCAAGA 
CGTATGGCGA TCAATCATTT TAACGCCGAG GGCAGCGATG ATTTCTGCTT CCTTTTGTCT 
ACCCGCGCCG GTGGCCTTGG AATCAATCTT ATGACGGCTG ATACTGTCAT CATCTATGAC 
TCGGACTGGA ATCCCCAGGC CGATCTTCAG GCAATGGCTC GAGCTCACCG TATCGGCCAG 
AAAAGACCTG TTAATGTCTA CCGCCTCGTG GCCAAGCAGA CTATTGAAGA GGAAGTTGTA 
AAGCGAGCCC GCAACAAGCT CTTCTTGGAG TATCTTACCA TTCAGGCCGG TGTGACAGAT 
GAAGGCAAGG CTCTCAAAGA GCAGTTCAAG GAGCGCGGCC TCAAGATGGA CGAGGCTAAG 
ACCGCTGAGG ATATCTCGAT GATCCTCAAG ATGCGTTCCC AGAACCTGTT CGAACAATCT 
AGCAACCAGC AGAAGCTGGA GCAGCTCGAT ATCGATGCTA TCCTCGAGAA TGCCGAAGTT 
ACCAAAACTG ATGTCGATGA CAAGATCAAC CTGAGCAGTG GTGGTATCGA CTGGGATAAC 
TGGATGCAGG TCACCGATGT CAAAGTTGAC GATCTCGCCC TTGACTGGGA TCAGATCATT 
CCAGCCGAGC AGCTTGCTGC CATCAAGGCT GAGGAAAATA GAAAGAAGGA AGAGGAGTAC 
TTGGCAAAAA CGATAGAGGA GAATGCTCCT AGAAAAGCTG CGTTGAAAGG CTCCAAAAAG 
AGCGCAGAGA ACGATCGAGC TGAGCGTCTG GCAAAGAAGC GACAACGCGA GAGGGAAGAG 
CAGGAAGAGC TCGAAGAGCA ACGTGCATTG CTTTCTGATC CCAAACGCCC CCTCAATGAG 
AAAGAAACCC GAAACCTTAT CAGGGCCTTC TTCAGGTACG GTTCCTTGGA CGACCGCGAA 
GAAGAGGTTG TCCAGGATGC CCGCCTTAGT GATAGAGATC GGGACTTTCT GAAGTCTATT 
ATCGAGGACC TTGTCGTTAT CAGTCGCCGC GCTGTAGATG CGAACAACGA GAGACTTCGC 
GAAGAAGAAG CCAGGGCTGG CAAGCCACTG GCGAAGAAGG ATAAGAAGGC TGTACTTGTC 
GATTTTGGCG AGGTGCGGAA AGTCAACGCA GAGACTGTCG TGGAGCGACC CCCTCAGCTC 
AAACTCCTGC GTCGCGTCCT CCGGGAGCAC GCAGACATTC TCAGTTTCAG GCTTCCCGAA 
GCTTCCAAGG CTGCCGCATA TACTTGCGAC TGGGGTGCAC GCGAGGATGC CATGCTGCTT 
ATTGGAATCG ACAGGTACGG ATTTGGTGCG TGGACGCAAA TCAGGGACGA TCCTGAGTTG 
CAGATGCACG ACAAGTTCTT CCTGGAGGAG CATCGTGTCG ACAAACGGGA AGAACGAAAG 
AAGACGGAGG CTGTAGGGAT TCAATCCCCT GGTGCTGTTC ATTTGGTCCG AAGGTCCGAG 
TATCTCCTGT CTGTTCTGTT GGCCAAGCAC TCTAATGATG CTGCCGCTAA GAAGGCAGTG 
GAGAACCACC ATCGGAGCAA GAAGCTTCTC ACGAACAACG GCCATCGACG CGGTGAAGCT 
AGCTCCATCT CCGGGTCTCC TGCACCATTG GGCGTTAAAA AGTCTATTCC TCACCGGGAC 
CACCACCATC GGACTCTCAG CAACGCGAAT GACCGGGGGA CCCCCAGGCC TGACCACAAA 
CGGAAGTACA CCGATGATTA CGAAGAGCGC AGCTCGAAGC ATCGTCGGGT GGAAGACGAA 
CGTCGTGGTA CAGATAGCAA ACTCGACAAG AAGAAGGACA AGGCACCCAA GGTCGATCCC 
GAAACATTGG AGAAATGGAA GCGGGACAGG CAGAAAGCGG TGGAGCGCTT CCATGAACTT 
GCCAAGTTGA ATAATGATGA GATCGATAGC TCGGACAACT TCCAACTTGT TTGGTCTCTA 
CTTCGGCCCC TCAAAGCGAA CATGGAACGC ATCATGTACT CTAAGGAACG TTTTCCTGCG 
GCCAAAGAAC GCGCCAAAAT TTTGGGAGAG GAGATTCGGG CATTTGGTAA TTTCCTTAAG 
CAGCTGGAGG AGAAGAACCC GCTGGATGGT TTGGAGCCAC AGTTCTGGGA ATTCCTTTCT 
TCGATCTGGC CCATCGGTGA GGTCAAGGTG TCGGGCCGAA GACTCCAGCG CATGTTCGGC 
GACCTTTGCA AAAAGGATAG CGAGAACAAG GAGGACAGAT CTCAGAATCT GAAGAAGCCG 
AGCCGCCAGG ATCTCGAAGA TGGCGAGATT GCCAGCGATC GCGACGATCG AAGATCTCGT 
GACCCTTACC GCGACGACCG GCGCGAGGAC CGGCGCGAAG AGCGACGAGA GGAGCCCAAG 
CGGCGCCCGA ACTATTACGA GAATGGCGAT CGACTCAACT TTTATCGCCA TCGTCGGGAC 
ATCGATAGGG GAGCACCGAT CCGTCACTCA TGGCAGAGCA GCCGCAGTCC CTCTCAGCAG 
CAACGGCCTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393067.1
CDS	352..5346
Translation	MSTSPESGPVFGHVSPPADDSHFNFTNDTESTSDLSDVNAAEADPNDDADAQSYQPADEAVSPIQKPELTFEEASDSASDNDASDDGDFDAPDSPRSAQSNEVVSASSSRTVVKRKVAHATEDDYIRENPALYGLRRSSRPTQRRAQIIDSDEEDEPSESDATPVSRKPAKRRRVERSLPASKRGTPARQPSIDDSDSDTYGGAKARSFQRKVRRQQEQDPALAFSEKRWSSRRAAQVSAGAYQESDAEEDEEDDSELTPNYWSANVEDNSPYIEKILGHRPKEGVEVTVDSTRHDFEYFIKWQGQSYPHNTWETTETVAGFRGFRRLENYYKKVVEYELEMQFGGDDISPEQKEQWYLDREREQEALEDYTKVERVVAVRDGDEGTEYYIKWKGLQYDECTWEAESLVAELAQDKINQFLDRCNRSWQSDRKQTNPDTRSRMTKLEKQPDYIKGGELREFQMKGLNFLALNWVRGNNVILADEMGLGKTVQSVSFLSWLRNERGQEGPFLVVAPLSVIPAWCDTFNHWAPDLNYVVYLGPEAARSNIRDYELFVDGNPKKTKFNVLVTSYDYILADADHLKNIKWQVLAVDEAHRLKNRESQLYIKLNNFGVPCKVLITGTPIQNNLAELSALLDFLNPGKVVIDEELEALSTADNKGPTDEEQDEARRIRTQEKLRELHQSIAPFILRRTKETVESDLPPKTEKIIRVELSDVQLDYYKNILTRNYAALSDASNGHKQSLLNIMMELKKVSNHPYMFQGAEERVLNGSTRREDQIKGLITSSGKMMLLDQLLAKLRKDGHRVLIFSQMVKMLDILGDYLRVRGYQFQRLDGTIPAGPRRMAINHFNAEGSDDFCFLLSTRAGGLGINLMTADTVIIYDSDWNPQADLQAMARAHRIGQKRPVNVYRLVAKQTIEEEVVKRARNKLFLEYLTIQAGVTDEGKALKEQFKERGLKMDEAKTAEDISMILKMRSQNLFEQSSNQQKLEQLDIDAILENAEVTKTDVDDKINLSSGGIDWDNWMQVTDVKVDDLALDWDQIIPAEQLAAIKAEENRKKEEEYLAKTIEENAPRKAALKGSKKSAENDRAERLAKKRQREREEQEELEEQRALLSDPKRPLNEKETRNLIRAFFRYGSLDDREEEVVQDARLSDRDRDFLKSIIEDLVVISRRAVDANNERLREEEARAGKPLAKKDKKAVLVDFGEVRKVNAETVVERPPQLKLLRRVLREHADILSFRLPEASKAAAYTCDWGAREDAMLLIGIDRYGFGAWTQIRDDPELQMHDKFFLEEHRVDKREERKKTEAVGIQSPGAVHLVRRSEYLLSVLLAKHSNDAAAKKAVENHHRSKKLLTNNGHRRGEASSISGSPAPLGVKKSIPHRDHHHRTLSNANDRGTPRPDHKRKYTDDYEERSSKHRRVEDERRGTDSKLDKKKDKAPKVDPETLEKWKRDRQKAVERFHELAKLNNDEIDSSDNFQLVWSLLRPLKANMERIMYSKERFPAAKERAKILGEEIRAFGNFLKQLEEKNPLDGLEPQFWEFLSSIWPIGEVKVSGRRLQRMFGDLCKKDSENKEDRSQNLKKPSRQDLEDGEIASDRDDRRSRDPYRDDRREDRREERREEPKRRPNYYENGDRLNFYRHRRDIDRGAPIRHSWQSSRSPSQQQRPY

Target ORF information:

RefSeq Version	XM_011394765.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A chromodomain helicase (crf6-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394765.1

ORF Insert Sequence:

ATGTCGACAT CTCCCGAATC CGGTCCGGTG TTCGGGCATG TGTCACCGCC GGCGGATGAC 
AGCCACTTCA ACTTTACCAA CGATACCGAA AGCACGAGCG ACCTTTCAGA CGTTAATGCC 
GCCGAGGCCG ACCCAAATGA CGACGCCGAC GCCCAGTCGT ATCAGCCTGC CGACGAGGCT 
GTCTCGCCGA TACAAAAACC CGAGTTGACG TTCGAGGAAG CGTCCGATTC AGCAAGTGAT 
AACGACGCGT CAGATGACGG AGACTTTGAC GCCCCCGATA GCCCCAGGTC TGCTCAGAGC 
AACGAAGTCG TGTCTGCCTC GTCGTCCCGT ACCGTCGTGA AGCGCAAAGT CGCCCACGCG 
ACCGAGGATG ATTACATCAG AGAGAATCCT GCCCTGTATG GGTTGAGGAG GAGTTCACGC 
CCTACACAAC GCCGTGCGCA GATCATCGAC TCAGATGAAG AAGATGAACC ATCCGAATCG 
GATGCCACAC CGGTGTCTAG AAAGCCTGCG AAGAGACGTC GGGTTGAACG CTCGTTGCCT 
GCTTCCAAGC GTGGCACTCC TGCTCGCCAA CCCTCAATCG ATGATTCCGA CTCGGACACA 
TATGGAGGCG CCAAAGCCCG CAGCTTCCAA AGAAAGGTCC GCAGGCAACA AGAACAGGAC 
CCTGCCTTGG CTTTTAGCGA AAAGAGATGG TCTAGCCGTC GGGCCGCCCA GGTCTCAGCC 
GGTGCCTACC AAGAAAGTGA CGCCGAAGAA GATGAAGAGG ACGACTCCGA GCTTACCCCC 
AACTACTGGT CTGCCAATGT CGAGGATAAC TCGCCATACA TCGAGAAAAT CCTCGGTCAT 
CGTCCTAAGG AGGGTGTCGA GGTCACGGTG GATTCTACGC GACACGACTT TGAATACTTC 
ATCAAGTGGC AGGGACAATC ATATCCCCAC AACACCTGGG AAACTACGGA AACTGTCGCA 
GGCTTCCGAG GCTTTCGCCG CCTCGAAAAC TATTACAAGA AGGTTGTTGA GTACGAACTG 
GAGATGCAAT TCGGTGGCGA CGATATCAGT CCGGAGCAGA AAGAGCAGTG GTACCTGGAT 
AGAGAACGTG AGCAAGAGGC CCTCGAAGAC TACACAAAGG TCGAGCGCGT TGTGGCTGTC 
AGAGACGGTG ATGAGGGCAC CGAGTATTAC ATCAAGTGGA AGGGCCTGCA ATACGACGAG 
TGCACCTGGG AAGCCGAGTC TCTCGTGGCC GAACTTGCTC AGGACAAGAT TAACCAGTTC 
CTCGACCGAT GCAACCGGTC ATGGCAGTCT GACCGCAAGC AAACTAACCC CGATACCCGG 
TCGAGGATGA CCAAACTGGA GAAGCAGCCT GACTATATCA AGGGCGGCGA GCTTCGCGAG 
TTCCAAATGA AAGGCCTTAA CTTCCTTGCT CTCAACTGGG TAAGGGGCAA CAACGTCATC 
TTAGCCGATG AAATGGGTCT GGGAAAGACT GTCCAAAGCG TCTCGTTCCT CAGCTGGTTA 
CGGAATGAAC GCGGCCAGGA AGGACCGTTC CTTGTCGTCG CTCCTCTTAG CGTCATCCCC 
GCTTGGTGCG ATACATTTAA TCATTGGGCT CCGGATCTCA ATTACGTCGT TTACCTGGGC 
CCGGAGGCAG CTCGGTCCAA CATTCGAGAT TACGAACTTT TTGTGGATGG TAACCCAAAG 
AAGACCAAGT TCAATGTCTT AGTCACTTCC TATGACTACA TCTTGGCCGA TGCGGATCAT 
CTCAAGAACA TCAAATGGCA AGTTCTCGCT GTTGACGAGG CCCACCGTCT CAAGAACCGA 
GAGTCACAGC TCTACATCAA GCTCAACAAC TTCGGCGTTC CTTGCAAGGT CCTCATTACC 
GGCACACCCA TTCAGAACAA TCTGGCCGAA CTCTCAGCCC TCCTCGACTT CCTTAACCCC 
GGAAAGGTCG TCATTGATGA GGAGCTTGAA GCGTTGTCTA CCGCAGATAA CAAAGGTCCC 
ACTGATGAAG AACAGGATGA GGCTAGGCGT ATCAGGACCC AAGAGAAGCT CCGCGAGTTG 
CATCAGTCAA TCGCTCCTTT CATTTTGCGT CGCACCAAGG AGACGGTCGA GTCGGACCTC 
CCCCCCAAAA CCGAAAAGAT CATCCGAGTT GAGTTGTCTG ACGTGCAACT TGACTACTAC 
AAGAACATTC TCACACGCAA CTATGCTGCC CTCAGCGACG CGAGCAACGG CCACAAGCAA 
TCGTTGCTCA ACATCATGAT GGAGCTGAAG AAAGTCAGTA ACCACCCGTA CATGTTCCAG 
GGTGCCGAGG AGCGTGTCCT CAACGGCAGC ACACGCCGTG AAGACCAGAT CAAGGGTCTC 
ATTACAAGTA GCGGCAAGAT GATGCTTCTT GATCAGCTGC TGGCAAAACT TAGGAAGGAC 
GGACATCGTG TTCTGATCTT CAGTCAGATG GTCAAGATGC TAGACATTTT GGGTGACTAT 
CTCCGTGTTC GCGGATATCA ATTCCAGCGA CTCGACGGTA CCATTCCTGC CGGCCCAAGA 
CGTATGGCGA TCAATCATTT TAACGCCGAG GGCAGCGATG ATTTCTGCTT CCTTTTGTCT 
ACCCGCGCCG GTGGCCTTGG AATCAATCTT ATGACGGCTG ATACTGTCAT CATCTATGAC 
TCGGACTGGA ATCCCCAGGC CGATCTTCAG GCAATGGCTC GAGCTCACCG TATCGGCCAG 
AAAAGACCTG TTAATGTCTA CCGCCTCGTG GCCAAGCAGA CTATTGAAGA GGAAGTTGTA 
AAGCGAGCCC GCAACAAGCT CTTCTTGGAG TATCTTACCA TTCAGGCCGG TGTGACAGAT 
GAAGGCAAGG CTCTCAAAGA GCAGTTCAAG GAGCGCGGCC TCAAGATGGA CGAGGCTAAG 
ACCGCTGAGG ATATCTCGAT GATCCTCAAG ATGCGTTCCC AGAACCTGTT CGAACAATCT 
AGCAACCAGC AGAAGCTGGA GCAGCTCGAT ATCGATGCTA TCCTCGAGAA TGCCGAAGTT 
ACCAAAACTG ATGTCGATGA CAAGATCAAC CTGAGCAGTG GTGGTATCGA CTGGGATAAC 
TGGATGCAGG TCACCGATGT CAAAGTTGAC GATCTCGCCC TTGACTGGGA TCAGATCATT 
CCAGCCGAGC AGCTTGCTGC CATCAAGGCT GAGGAAAATA GAAAGAAGGA AGAGGAGTAC 
TTGGCAAAAA CGATAGAGGA GAATGCTCCT AGAAAAGCTG CGTTGAAAGG CTCCAAAAAG 
AGCGCAGAGA ACGATCGAGC TGAGCGTCTG GCAAAGAAGC GACAACGCGA GAGGGAAGAG 
CAGGAAGAGC TCGAAGAGCA ACGTGCATTG CTTTCTGATC CCAAACGCCC CCTCAATGAG 
AAAGAAACCC GAAACCTTAT CAGGGCCTTC TTCAGGTACG GTTCCTTGGA CGACCGCGAA 
GAAGAGGTTG TCCAGGATGC CCGCCTTAGT GATAGAGATC GGGACTTTCT GAAGTCTATT 
ATCGAGGACC TTGTCGTTAT CAGTCGCCGC GCTGTAGATG CGAACAACGA GAGACTTCGC 
GAAGAAGAAG CCAGGGCTGG CAAGCCACTG GCGAAGAAGG ATAAGAAGGC TGTACTTGTC 
GATTTTGGCG AGGTGCGGAA AGTCAACGCA GAGACTGTCG TGGAGCGACC CCCTCAGCTC 
AAACTCCTGC GTCGCGTCCT CCGGGAGCAC GCAGACATTC TCAGTTTCAG GCTTCCCGAA 
GCTTCCAAGG CTGCCGCATA TACTTGCGAC TGGGGTGCAC GCGAGGATGC CATGCTGCTT 
ATTGGAATCG ACAGGTACGG ATTTGGTGCG TGGACGCAAA TCAGGGACGA TCCTGAGTTG 
CAGATGCACG ACAAGTTCTT CCTGGAGGAG CATCGTGTCG ACAAACGGGA AGAACGAAAG 
AAGACGGAGG CTGTAGGGAT TCAATCCCCT GGTGCTGTTC ATTTGGTCCG AAGGTCCGAG 
TATCTCCTGT CTGTTCTGTT GGCCAAGCAC TCTAATGATG CTGCCGCTAA GAAGGCAGTG 
GAGAACCACC ATCGGAGCAA GAAGCTTCTC ACGAACAACG GCCATCGACG CGGTGAAGCT 
AGCTCCATCT CCGGGTCTCC TGCACCATTG GGCGTTAAAA AGTCTATTCC TCACCGGGAC 
CACCACCATC GGACTCTCAG CAACGCGAAT GACCGGGGGA CCCCCAGGCC TGACCACAAA 
CGGAAGTACA CCGATGATTA CGAAGAGCGC AGCTCGAAGC ATCGTCGGGT GGAAGACGAA 
CGTCGTGGTA CAGATAGCAA ACTCGACAAG AAGAAGGACA AGGCACCCAA GGTCGATCCC 
GAAACATTGG AGAAATGGAA GCGGGACAGG CAGAAAGCGG TGGAGCGCTT CCATGAACTT 
GCCAAGTTGA ATAATGATGA GATCGATAGC TCGGACAACT TCCAACTTGT TTGGTCTCTA 
CTTCGGCCCC TCAAAGCGAA CATGGAACGC ATCATGTACT CTAAGGAACG TTTTCCTGCG 
GCCAAAGAAC GCGCCAAAAT TTTGGGAGAG GAGATTCGGG CATTTGGTAA TTTCCTTAAG 
CAGCTGGAGG AGAAGAACCC GCTGGATGGT TTGGAGCCAC AGTTCTGGGA ATTCCTTTCT 
TCGATCTGGC CCATCGGTGA GGTCAAGGTG TCGGGCCGAA GACTCCAGCG CATGTTCGGC 
GACCTTTGCA AAAAGGATAG CGAGAACAAG GAGGACAGAT CTCAGAATCT GAAGAAGCCG 
AGCCGCCAGG ATCTCGAAGA TGGCGAGATT GCCAGCGATC GCGACGATCG AAGATCTCGT 
GACCCTTACC GCGACGACCG GCGCGAGGAC CGGCGCGAAG AGCGACGAGA GGAGCCCAAG 
CGGCGCCCGA ACTATTACGA GAATGGCGAT CGACTCAACT TTTATCGCCA TCGTCGGGAC 
ATCGATAGGG GAGCACCGAT CCGTCACTCA TGGCAGAGCA GCCGCAGTCC CTCTCAGCAG 
CAACGGCCTT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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crf6-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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