NCU07832 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU07832
Entrez Gene ID	3879101
Full Name	pre-mRNA processing splicing factor 8
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_957860.2	XP_962953.1	pre-mRNA processing splicing factor 8

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Neurospora crassa	NCU07832	XP_962953.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Homo sapiens (human)	PRPF8	NP_006436.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU07832 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU07832 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf01605	XM_957860.2 Latest version!	Neurospora crassa OR74A pre-mRNA processing splicing factor 8 (NCU07832), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU07832 ( XM_957860.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_962953.1 Neurospora crassa OR74A pre-mRNA processing splicing factor 8 (NCU07832), mRNA.

Price & Availability↑

CloneID	ONf01605
Clone ID Related Accession (Same CDS sequence)	XM_957860.2
Accession Version	XM_957860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	pre-mRNA processing splicing factor 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_957860.1.

ATGTCCTATC CGCCACCACC ACCTCCTCCC CCCGGCTGGG GCGCTCCTCC GCCGCCATCA 
GCACCTCCGC CGCCGCCGCC TCCGTCCTCT CTCCCACCTC CTCCTGCCCT CCCAGCACCG 
CCGCCGCCTG GGTTCTTTCC ACCAACCGAC CCTCATGTTG CCAAGTTCGC CCAGAAGAAG 
AAGGAATGGC TTCGCATGCA GAGAAATCGC TTCGGCGAGA AGAGGAAGGG CGGATTTGTT 
GAGACGGAAA AGGCCGACAT GCCACCAGAG CACTTGCGGA AGATCGTCAA GGATATTGGG 
GATGTTTCGC AGAAAAAGTA CACCAGTGAC AAGCGCAGCT ATCTTGGGGC GCTCAAGTTC 
ATGCCCCATG CTGTGCTGAA GCTGCTCGAG AACATGCCCA TGCCTTGGGA GGCTGCCAGA 
GAGGTCAAGG TTCTCTATCA CGTCAACGGC TGTCTGACCC TTGTGAATGA GATCCCGAGA 
GTCATTGAGC CCGTCTTCTT TGCACAGTGG GCCAGTATGT GGACGGTAAT GCGCAAGGAA 
AAGAGCGACC GCCGGCTATT CAAGCGCATG CGATTTCCAC CTTTCGACGA CGAAGAACCG 
CCCCTGTCCT GGTCCGAGAA CATCGAGGAC GTCGAGCCAC TTGAACCCAT TCAGATGGAG 
TTGGATGAGG ACGAAGAAGC AGCAATCTAT GAGTGGTTCT ATGACCACAA GCCGCTTCTC 
GACACGCCCC ACGTCAACGG GCCCAGTTAC AAGAAGTGGA ATCTCACCCT GCCGCAAATG 
GCGAGTTTAT ACCGTCTAAG CCGACCGTTG GTGTCTGAGG TTGTCGACAA GAACTACTAC 
TACCTCTTTG AGCTCAAGAG TTTCCTCACA GCAAAGGCCC TGAATGCGGC TTTACCAGGC 
GGGCCTCGCT TCGAGCCACT TTACAAGGAC ATTGACCCCA ATGATGAGGA TTTTGGCGAG 
TTCAATGCTA TGGATCGCAT CATCTTCAGG AACCCTATCC GAACAGAGTG CCGTGTAGCT 
TACCCCCATC TTTATAATGC ACTTCCGAGA AGCGTCCAGT TGTCAGTCCA CTCATACCCG 
CAAGTTGTCT ATACGCGCAC AGAAGACTAC AACCTTCCCG CTTTTTACTT TGACACGAGC 
ATCAACCCGA TTTCTTCGCG GGCTGTTGCC CCCAAGAACC TTACAGTCAG CCATGAAGAT 
GAGATCTTTG GACCTGGCAA TATCGAGGAG CCGGAAGAAG ATGCGTTTGA GCTGCCCGCT 
GGTGTCGAGT CTTTCATGGC CGAGGAATCG CTGTATACGG ATGAGACGGC ATCGGCCATC 
GAGCTTTGGT GGGCGCCGTT CCCCTTCGAC CGCCGCTCTG GACGTACGGT TCGTGCCCAG 
GATGTACCTC TCGTAAAGCA TTGGTACTTG GAGCACTGCC CGCCGAAGCA GCCCGTTAAG 
GTGCGCGTCT CGTACCAGAA GCTTCTCAAG AACTACGTCC TCAATGAGCT GCACAAGAAG 
CATCCAAAAT CTCTTCAGAA GCAAAACTTG CTGCGGACTT TGAAACAAAC AAAGTTCTTC 
CAGCAGACAA CGATCGATTG GGTTGAAGCC GGCTTGCAAG TCTGCCGACA GGGCTTTAAC 
ATGCTCAACC TGCTTATCCA CCGCAAGAAC CTCACATATC TCCATTTGGA CTACAACTTC 
AACCTGAAGC CCGTCAAGAC GCTGACAACC AAGGAGCGAA AGAAGTCTAG ATTCGGAAAT 
GCCTTCCATC TTATGCGTGA AATTCTGCGC TTGACCAAGC TCATTGTCGA TGCCCAGGTA 
CAGTACCGTC TTGGCAACAT TGACGCCTTC CAGCTTGCCG ATGGTATTCT GTATGCCTTC 
AACCATGTCG GACAGTTGAC CGGTATGTAT CGTTACAAGT ACAAGTTGAT GCACCAGATC 
CGTTCCTGCA AGGATCTCAA GCATCTCATC TACTATCGCT TCAACGCCGG CCCGGTTGGC 
AAGGGTCCAG GCTGCGGTTT CTGGGCGCCG GCATGGCGTG TGTGGCTCTT CTTCATGCGC 
GGTATCATTC CCCTTCTTGA AAGATGGCTT GGCAACCTCC TTTCCCGTCA GTTCGAAGGT 
CGCCACAGCA AGGGAGTTGC CAAGACCGTT ACCAAGCAGC GTGTTGAATC TCACTTCGAT 
CTCGAGCTTC GTGCTTCCGT CATGGCTGAT CTGCTGGACA TGATGCCGGA AGGTATCAAG 
CAGAGCAAGG TCAACACGGT TCTTCAACAT CTTTCTGAGG CCTGGAGATG CTGGAAGAGT 
AACATTCCTT GGAAGGTTCC TGGACTTCCC GCAGCTATTG AGAACATCAT TCTGCGTTAC 
GTCAAGTCCA AGGCCGATTG GTGGATTTCG GTTGCTCATT ACAACCGGGA GCGTATTCGT 
CGGGGTGCCA CCGTCGATAA GACCGTTGCT AAGAAGAACG TTGGCCGCCT AACGCGTCTG 
TGGCTCAAGG CCGAGCAAGA ACGCCAGCAC AACCACATGA AGGATGGTCC CTACGTCTCT 
TCTGAGGAGG CTGTTGCCAT TTACACTACT ACGGTGCACT GGCTTGAATC GAGAAAATTC 
TCTCCCATCC CCTTCCCCAG TGTGTCTTAC AAGCATGACA CCAAGATCCT TATCTTGGCG 
CTGGAGCGCC TACGTGAGGC TTACTCGACC AAGGGAAGAT TGAACCAGAG TCAGCGTGAG 
GAGTTGGCCT TGATTGAGCA GGCATATGAC AGTCCAGGCA CTACCCTCGA GCGTATCAAA 
CGCTTCCTAC TCACTCAAAG AGCTTTCAAG GAAGTCGGCA TCGATATGAA CGACAACTAC 
AGCACCATCA ACCCGGTCTA TGATATTGAA CCGATCGAGA AGATTAGCGA TGCTTATCTC 
GACCAGTACT TGTGGTATCA AGCTGACCAG CGCCATCTGT TCCCCGCTTG GATTAAGCCT 
TCCGATTCGG AGGTGCCACC TTTGCTTGTG TACAAGTGGG CTCAAGGCAT TAACAACCTC 
GACAGGGTTT GGGAGACCGC GAATGGCGAG TGCAATGTCA TGATCGAGAC GCAGCTCTCC 
AAGGTCTACG AGAAGATTGA GCTGACCTTG CTCAACTCGC TTCTTCGGTT GATAATGGAC 
CACAACCTTG CGGACTACAT CACGGCCAAG AATAACGTTA CCCTAACTTA CAAGGACATG 
AACCATGTCA ACAGCTATGG TATGATTCGT GGCCTGCAGT TCTCAGCCTT CGTTTTCCAG 
TACTATGGTC TCATTCTCGA TTTGCTGTTG CTTGGCCCGC AGCGTGCCAG CGAGATTGCC 
GGTCCTCCCC ACGCACCCAA TGACTTCCTT CAGTTCAAGG ACCGGGAGAC TGAAACAAGA 
CACCCGATTA GGTTGTACAC CAGGTACATC GACAAGATCT GGGTGTTCTT GAGGTTCACC 
GCGGATGAAT CTCGCGACCT GATCCAGCGA TTCTTGACAG AGCAGCCTGA TCCCAACTTC 
GAGAACGTGA TTGGTTACAA GAGCAAGAAG TGCTGGCCCA GAGATTCGCG TATGCGTCTC 
ATGAGGCACG ACGTCAACCT CGGCAGAGCT GTGTTCTGGG ATCTCAAGAA CCGCCTCCCA 
AGATCCGTGA CGACGATTGA ATGGGAAGAC ACCTTCGCCA GCGTGTACAG CAGGGATAAC 
CCGAACCTCC TGTTCTCCAT GTGCGGCTTC GAAGTGCGCA TCTTGCCCAA GATCCGCAAC 
CAGAACGATG AGTTCCCTGT GAAAGACAGT GTCTGGTCAC TAGTGGACAA CACGACCAAG 
GAACGCACCG CTCACGCTTT CCTACAAGTT ACCGAAGAGG ACATTCAGAA ATTCAATAAC 
AGGATCCGTC AGATTCTCAT GTCGTCTGGC TCGACCACAT TCACCAAGAT TGCTAACAAG 
TGGAACACGG CGCTGATTGC CCTCTTTACT TACTACCGCG AGGCTGCTGT TTCGACCGTG 
AACCTGCTTG ACACGATTGT CAAGTGTGAA ACCAAAATCC AGACTCGTGT CAAAATTGGT 
CTCAACTCCA AGATGCCTTC CCGTTTCCCT CCTGCCGTTT TCTACACTCC CAAGGAGCTT 
GGTGGCTTGG GTATGATTTC CGGATCACAC ATCCTTATCC CTACTAGCGA CAAGCGCTGG 
TCTAAACAGA CAGATCTCGG AGTTACTCAT TACCGTGCGG GCATGTCGCA CGATGAGGAA 
ACGCTGATCC CCAACATCTT CCGCTACATC ATCCCGTGGG AAGCTGAGTT TATCGACTCC 
CAGCGTGTGT GGACGGAATA CTCGCAGAAG CGTCTCGAGG CCAACCAGCA GAACCGCAGA 
TTGACCCTAG AGGATCTGGA GGACAGCTGG GATCGCGGTC TGCCCCGTAT AAACACTCTT 
TTTCAAAAGG ACCGTAGCAC GTTAAGCTTC GACAAGGGTT TCCGTGCCCG TTCCGAGTTC 
AAGATCTATC AGTTGATGAA GAGCAATCCC TTCTGGTGGA CAAGCCAGAG GCATGACGGT 
AAACTTTGGA ACCTTAACGC ATACCGTACC GATGTCATCC AGGCGCTTGG TGGTGTCGAA 
ACCATCTTGG AGCATACCTT GTTCAAGGCA ACTGGTTTCC CGTCTTGGGA AGGTCTCTTC 
TGGGAGAAGG CCAGTGGTTT CGAGGAGTCG ATGAAGTTCA AGAAGTTGAC AAATGCCCAG 
AGGAGCGGTC TCAACCAGAT TCCTAATCGT CGTTTCACGC TTTGGTGGTC GCCTACCATC 
AACAGAGCCA ATGTCTACGT CGGTTTCCAA GTGCAGTTGG ATCTAACTGG TATCTTCTTG 
CACGGCAAGA TTCCTACTCT CAAGATCTCC CTGATCCAGA TCTTCCGAGC TCATCTGTGG 
CAGAAGATCC ACGAGTCAGT GGTCATGGAT CTTTGCCAGG TCTTCGATCA GGAGCTTGAG 
GCTCTAAGCA TCGAATCGGT TCAAAAGGAA ACCATTCATC CCAGAAAATC TTACAAGATG 
AACAGCTCCT GCGCGGATAT CCAGCTCTTT GCCAGCCACA AATGGAACGT TACTCGCCCA 
TCGCTCCTTT TCGATACCAA GGATGTCATC GAGTCTACTA CGACGAACAA GTTTTGGATT 
GATGTTCAGC TTCGCTATGG TGACTACGAC TCTCACGATA TTGAGAGATA CGTCAGGGCC 
AAGTATCTGG ACTACACCAC CGACAGCATG AGTCTTTACC CATCTCCTAC CGGTCTCATG 
ATTGGCATCG ATCTGGCTTA CAACTTGTAC TCTGCCTACG GTCAATACTT CCCAGGCTTG 
AAGCTCTTGA TCCAGCAGGC TATGGGCAAG ATCATGAAGG CAAACCCTGC GCTCTACGTT 
CTTCGTGAGC GTATCCGCAA GGGTCTGCAG CTCTACGCCT CCGAGAGCAA CCAGGAGTTC 
CTGAATTCGC AGAACTACTC GGAGCTGTTC AGCAACCAGA CTCAGCTCTT TATCGATGAT 
ACTAACGTCT ACCGTGTTAC CATCCACAAG ACCTTCGAAG GTAACTTGAC GACCAAGCCG 
ATCAACGGTG CCATCTTCAT CTTCAACCCG CGTACCGGTC AGTTGTTCTT GAAGATTATC 
CACACCAGCG TTTGGGCGGG TCAGAAGCGT TTGGGACAGT TGGCCAAGTG GAAGACTGCC 
GAAGAAGTCG CAGCCCTTAT CAGATCGCTA CCCGTCGAAG AGCAGCCAAA GCAACTGATC 
GTCACCAGAA AGGGTCTGTT GGATCCTTTG GAAGTCAATT TGCTTGATTT CCCCAACATC 
TCCATCCGCG CGTCCGAGTT GCAGCTTCCC TTCCAGGCCG CCATGAAGGT CGAAAAACTG 
GGTGACATGA TCTTGCGTGC CACCGAGCCG CAGATGGTCT TGTTCAATCT CTACGACGAG 
TGGCTGAAGA GCATCTCTTC CTACACTGCC TTCTCCCGTC TCATTCTCAT CCTGCGTGCC 
CTGCACGTTA ACCAGGATAA GACGAAGCTC ATCTTGCGCC CCGACAAGAC CGTCATCACG 
CAGGATCATC ACATCTGGCC CTCGCTCTCC GATGAAGACT GGATCAAGGT TGAAACGCAG 
CTCCGCGATC TTATCCTCAA CGACTACGGC AAGAAGAACA ATGTCAATGT GTCCAGTTTA 
ACTAGCAGTG AAGTCCGTGA TATCATTCTT GGTATGGAGA TCTCAGCCCC CTCGCTGCAG 
AGGCAGCAGG CGGCGGAGAT TGAGAAGCAG CAGCAAGAGC AGCAGCAGCT CACCGCCGTC 
ACTACCAAGA CGCAGAACGT GCACGGCGAG GAGATCATTG TGACGACCAC TTCGCAGTTC 
GAACAGCAGA CGTTCGCCTC CAAGACCGAG TGGCGCACCA GAGCCATCGC CACCTCGAAC 
CTACGCACCA GGGCTAACAA CATGTACGTG TCGCCCGTGG ATAGTGACCT GGACGACGTG 
ACCTATGTCA TGCCCAAGAA CATCCTCAAG CGCTTCATCG CCATTGCCGA TCTCCGTGTC 
CAAGTCGCCG GTTACCTCTA CGGTGCCTCG CCCGCCGACA ACGACCAGGT CAAGGAGATC 
AAGTGCATCG TCATGGTGCC TCAGATCGGT GGTCTGCGCA ACGTTCAGTT GCCTCACCAG 
CTTCCCCAGC ACGAGTACCT CAAGGACATG GAGCCACTCG GCATCATTCA CACACAGTCG 
GGCAACGAGC TGCCGTACAT GTCCGCCATG GACGTTACGG AGCACGCACG CCTGCTTGAT 
GCCCACCCGA ATTGGGACAA GCAAAACACA CTCACCGTCG CCGTCTCCTT TACGCCCGGT 
AGCGTGTCGC TGTCGGCCTG GGCGCTCACG CCTCAGGGCT TCAAGTGGGG CGTGGAGAAC 
AAGGACATAG CCAGCGACCA GCCGCAGGGG TTCACAACCA GCATGGGCGA AAAGCGCCAG 
CTCCTCCTCA GCGAGAAGTT CCGGGGCTTT TTCCTGGTGC CGGATGGCGG CAAGTGGAAC 
TACAGCTTCA TGGGCAGCGC GTTTGGAGGC CTGGAGAAAA AGCCGGTGCA TGTCAAGCTG 
GATACGCCGC TGCCCTTTTA CAGCGACCAG CACAGACCTA TTCACTTCTC GAGCTTCAAC 
GAGTTAGAGG ATATCTGGGT TGATAGGCAG GATAACTTTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_962953.1
CDS	482..7606
Translation	MSYPPPPPPPPGWGAPPPPSAPPPPPPPSSLPPPPALPAPPPPGFFPPTDPHVAKFAQKKKEWLRMQRNRFGEKRKGGFVETEKADMPPEHLRKIVKDIGDVSQKKYTSDKRSYLGALKFMPHAVLKLLENMPMPWEAAREVKVLYHVNGCLTLVNEIPRVIEPVFFAQWASMWTVMRKEKSDRRLFKRMRFPPFDDEEPPLSWSENIEDVEPLEPIQMELDEDEEAAIYEWFYDHKPLLDTPHVNGPSYKKWNLTLPQMASLYRLSRPLVSEVVDKNYYYLFELKSFLTAKALNAALPGGPRFEPLYKDIDPNDEDFGEFNAMDRIIFRNPIRTECRVAYPHLYNALPRSVQLSVHSYPQVVYTRTEDYNLPAFYFDTSINPISSRAVAPKNLTVSHEDEIFGPGNIEEPEEDAFELPAGVESFMAEESLYTDETASAIELWWAPFPFDRRSGRTVRAQDVPLVKHWYLEHCPPKQPVKVRVSYQKLLKNYVLNELHKKHPKSLQKQNLLRTLKQTKFFQQTTIDWVEAGLQVCRQGFNMLNLLIHRKNLTYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLMREILRLTKLIVDAQVQYRLGNIDAFQLADGILYAFNHVGQLTGMYRYKYKLMHQIRSCKDLKHLIYYRFNAGPVGKGPGCGFWAPAWRVWLFFMRGIIPLLERWLGNLLSRQFEGRHSKGVAKTVTKQRVESHFDLELRASVMADLLDMMPEGIKQSKVNTVLQHLSEAWRCWKSNIPWKVPGLPAAIENIILRYVKSKADWWISVAHYNRERIRRGATVDKTVAKKNVGRLTRLWLKAEQERQHNHMKDGPYVSSEEAVAIYTTTVHWLESRKFSPIPFPSVSYKHDTKILILALERLREAYSTKGRLNQSQREELALIEQAYDSPGTTLERIKRFLLTQRAFKEVGIDMNDNYSTINPVYDIEPIEKISDAYLDQYLWYQADQRHLFPAWIKPSDSEVPPLLVYKWAQGINNLDRVWETANGECNVMIETQLSKVYEKIELTLLNSLLRLIMDHNLADYITAKNNVTLTYKDMNHVNSYGMIRGLQFSAFVFQYYGLILDLLLLGPQRASEIAGPPHAPNDFLQFKDRETETRHPIRLYTRYIDKIWVFLRFTADESRDLIQRFLTEQPDPNFENVIGYKSKKCWPRDSRMRLMRHDVNLGRAVFWDLKNRLPRSVTTIEWEDTFASVYSRDNPNLLFSMCGFEVRILPKIRNQNDEFPVKDSVWSLVDNTTKERTAHAFLQVTEEDIQKFNNRIRQILMSSGSTTFTKIANKWNTALIALFTYYREAAVSTVNLLDTIVKCETKIQTRVKIGLNSKMPSRFPPAVFYTPKELGGLGMISGSHILIPTSDKRWSKQTDLGVTHYRAGMSHDEETLIPNIFRYIIPWEAEFIDSQRVWTEYSQKRLEANQQNRRLTLEDLEDSWDRGLPRINTLFQKDRSTLSFDKGFRARSEFKIYQLMKSNPFWWTSQRHDGKLWNLNAYRTDVIQALGGVETILEHTLFKATGFPSWEGLFWEKASGFEESMKFKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESVVMDLCQVFDQELEALSIESVQKETIHPRKSYKMNSSCADIQLFASHKWNVTRPSLLFDTKDVIESTTTNKFWIDVQLRYGDYDSHDIERYVRAKYLDYTTDSMSLYPSPTGLMIGIDLAYNLYSAYGQYFPGLKLLIQQAMGKIMKANPALYVLRERIRKGLQLYASESNQEFLNSQNYSELFSNQTQLFIDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQLIVTRKGLLDPLEVNLLDFPNISIRASELQLPFQAAMKVEKLGDMILRATEPQMVLFNLYDEWLKSISSYTAFSRLILILRALHVNQDKTKLILRPDKTVITQDHHIWPSLSDEDWIKVETQLRDLILNDYGKKNNVNVSSLTSSEVRDIILGMEISAPSLQRQQAAEIEKQQQEQQQLTAVTTKTQNVHGEEIIVTTTSQFEQQTFASKTEWRTRAIATSNLRTRANNMYVSPVDSDLDDVTYVMPKNILKRFIAIADLRVQVAGYLYGASPADNDQVKEIKCIVMVPQIGGLRNVQLPHQLPQHEYLKDMEPLGIIHTQSGNELPYMSAMDVTEHARLLDAHPNWDKQNTLTVAVSFTPGSVSLSAWALTPQGFKWGVENKDIASDQPQGFTTSMGEKRQLLLSEKFRGFFLVPDGGKWNYSFMGSAFGGLEKKPVHVKLDTPLPFYSDQHRPIHFSSFNELEDIWVDRQDNFA

Target ORF information:

RefSeq Version	XM_957860.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A pre-mRNA processing splicing factor 8 (NCU07832), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_957860.2

ORF Insert Sequence:

ATGTCCTATC CGCCACCACC ACCTCCTCCC CCCGGCTGGG GCGCTCCTCC GCCGCCATCA 
GCACCTCCGC CGCCGCCGCC TCCGTCCTCT CTCCCACCTC CTCCTGCCCT CCCAGCACCG 
CCGCCGCCTG GGTTCTTTCC ACCAACCGAC CCTCATGTTG CCAAGTTCGC CCAGAAGAAG 
AAGGAATGGC TTCGCATGCA GAGAAATCGC TTCGGCGAGA AGAGGAAGGG CGGATTTGTT 
GAGACGGAAA AGGCCGACAT GCCACCAGAG CACTTGCGGA AGATCGTCAA GGATATTGGG 
GATGTTTCGC AGAAAAAGTA CACCAGTGAC AAGCGCAGCT ATCTTGGGGC GCTCAAGTTC 
ATGCCCCATG CTGTGCTGAA GCTGCTCGAG AACATGCCCA TGCCTTGGGA GGCTGCCAGA 
GAGGTCAAGG TTCTCTATCA CGTCAACGGC TGTCTGACCC TTGTGAATGA GATCCCGAGA 
GTCATTGAGC CCGTCTTCTT TGCACAGTGG GCCAGTATGT GGACGGTAAT GCGCAAGGAA 
AAGAGCGACC GCCGGCTATT CAAGCGCATG CGATTTCCAC CTTTCGACGA CGAAGAACCG 
CCCCTGTCCT GGTCCGAGAA CATCGAGGAC GTCGAGCCAC TTGAACCCAT TCAGATGGAG 
TTGGATGAGG ACGAAGAAGC AGCAATCTAT GAGTGGTTCT ATGACCACAA GCCGCTTCTC 
GACACGCCCC ACGTCAACGG GCCCAGTTAC AAGAAGTGGA ATCTCACCCT GCCGCAAATG 
GCGAGTTTAT ACCGTCTAAG CCGACCGTTG GTGTCTGAGG TTGTCGACAA GAACTACTAC 
TACCTCTTTG AGCTCAAGAG TTTCCTCACA GCAAAGGCCC TGAATGCGGC TTTACCAGGC 
GGGCCTCGCT TCGAGCCACT TTACAAGGAC ATTGACCCCA ATGATGAGGA TTTTGGCGAG 
TTCAATGCTA TGGATCGCAT CATCTTCAGG AACCCTATCC GAACAGAGTG CCGTGTAGCT 
TACCCCCATC TTTATAATGC ACTTCCGAGA AGCGTCCAGT TGTCAGTCCA CTCATACCCG 
CAAGTTGTCT ATACGCGCAC AGAAGACTAC AACCTTCCCG CTTTTTACTT TGACACGAGC 
ATCAACCCGA TTTCTTCGCG GGCTGTTGCC CCCAAGAACC TTACAGTCAG CCATGAAGAT 
GAGATCTTTG GACCTGGCAA TATCGAGGAG CCGGAAGAAG ATGCGTTTGA GCTGCCCGCT 
GGTGTCGAGT CTTTCATGGC CGAGGAATCG CTGTATACGG ATGAGACGGC ATCGGCCATC 
GAGCTTTGGT GGGCGCCGTT CCCCTTCGAC CGCCGCTCTG GACGTACGGT TCGTGCCCAG 
GATGTACCTC TCGTAAAGCA TTGGTACTTG GAGCACTGCC CGCCGAAGCA GCCCGTTAAG 
GTGCGCGTCT CGTACCAGAA GCTTCTCAAG AACTACGTCC TCAATGAGCT GCACAAGAAG 
CATCCAAAAT CTCTTCAGAA GCAAAACTTG CTGCGGACTT TGAAACAAAC AAAGTTCTTC 
CAGCAGACAA CGATCGATTG GGTTGAAGCC GGCTTGCAAG TCTGCCGACA GGGCTTTAAC 
ATGCTCAACC TGCTTATCCA CCGCAAGAAC CTCACATATC TCCATTTGGA CTACAACTTC 
AACCTGAAGC CCGTCAAGAC GCTGACAACC AAGGAGCGAA AGAAGTCTAG ATTCGGAAAT 
GCCTTCCATC TTATGCGTGA AATTCTGCGC TTGACCAAGC TCATTGTCGA TGCCCAGGTA 
CAGTACCGTC TTGGCAACAT TGACGCCTTC CAGCTTGCCG ATGGTATTCT GTATGCCTTC 
AACCATGTCG GACAGTTGAC CGGTATGTAT CGTTACAAGT ACAAGTTGAT GCACCAGATC 
CGTTCCTGCA AGGATCTCAA GCATCTCATC TACTATCGCT TCAACGCCGG CCCGGTTGGC 
AAGGGTCCAG GCTGCGGTTT CTGGGCGCCG GCATGGCGTG TGTGGCTCTT CTTCATGCGC 
GGTATCATTC CCCTTCTTGA AAGATGGCTT GGCAACCTCC TTTCCCGTCA GTTCGAAGGT 
CGCCACAGCA AGGGAGTTGC CAAGACCGTT ACCAAGCAGC GTGTTGAATC TCACTTCGAT 
CTCGAGCTTC GTGCTTCCGT CATGGCTGAT CTGCTGGACA TGATGCCGGA AGGTATCAAG 
CAGAGCAAGG TCAACACGGT TCTTCAACAT CTTTCTGAGG CCTGGAGATG CTGGAAGAGT 
AACATTCCTT GGAAGGTTCC TGGACTTCCC GCAGCTATTG AGAACATCAT TCTGCGTTAC 
GTCAAGTCCA AGGCCGATTG GTGGATTTCG GTTGCTCATT ACAACCGGGA GCGTATTCGT 
CGGGGTGCCA CCGTCGATAA GACCGTTGCT AAGAAGAACG TTGGCCGCCT AACGCGTCTG 
TGGCTCAAGG CCGAGCAAGA ACGCCAGCAC AACCACATGA AGGATGGTCC CTACGTCTCT 
TCTGAGGAGG CTGTTGCCAT TTACACTACT ACGGTGCACT GGCTTGAATC GAGAAAATTC 
TCTCCCATCC CCTTCCCCAG TGTGTCTTAC AAGCATGACA CCAAGATCCT TATCTTGGCG 
CTGGAGCGCC TACGTGAGGC TTACTCGACC AAGGGAAGAT TGAACCAGAG TCAGCGTGAG 
GAGTTGGCCT TGATTGAGCA GGCATATGAC AGTCCAGGCA CTACCCTCGA GCGTATCAAA 
CGCTTCCTAC TCACTCAAAG AGCTTTCAAG GAAGTCGGCA TCGATATGAA CGACAACTAC 
AGCACCATCA ACCCGGTCTA TGATATTGAA CCGATCGAGA AGATTAGCGA TGCTTATCTC 
GACCAGTACT TGTGGTATCA AGCTGACCAG CGCCATCTGT TCCCCGCTTG GATTAAGCCT 
TCCGATTCGG AGGTGCCACC TTTGCTTGTG TACAAGTGGG CTCAAGGCAT TAACAACCTC 
GACAGGGTTT GGGAGACCGC GAATGGCGAG TGCAATGTCA TGATCGAGAC GCAGCTCTCC 
AAGGTCTACG AGAAGATTGA GCTGACCTTG CTCAACTCGC TTCTTCGGTT GATAATGGAC 
CACAACCTTG CGGACTACAT CACGGCCAAG AATAACGTTA CCCTAACTTA CAAGGACATG 
AACCATGTCA ACAGCTATGG TATGATTCGT GGCCTGCAGT TCTCAGCCTT CGTTTTCCAG 
TACTATGGTC TCATTCTCGA TTTGCTGTTG CTTGGCCCGC AGCGTGCCAG CGAGATTGCC 
GGTCCTCCCC ACGCACCCAA TGACTTCCTT CAGTTCAAGG ACCGGGAGAC TGAAACAAGA 
CACCCGATTA GGTTGTACAC CAGGTACATC GACAAGATCT GGGTGTTCTT GAGGTTCACC 
GCGGATGAAT CTCGCGACCT GATCCAGCGA TTCTTGACAG AGCAGCCTGA TCCCAACTTC 
GAGAACGTGA TTGGTTACAA GAGCAAGAAG TGCTGGCCCA GAGATTCGCG TATGCGTCTC 
ATGAGGCACG ACGTCAACCT CGGCAGAGCT GTGTTCTGGG ATCTCAAGAA CCGCCTCCCA 
AGATCCGTGA CGACGATTGA ATGGGAAGAC ACCTTCGCCA GCGTGTACAG CAGGGATAAC 
CCGAACCTCC TGTTCTCCAT GTGCGGCTTC GAAGTGCGCA TCTTGCCCAA GATCCGCAAC 
CAGAACGATG AGTTCCCTGT GAAAGACAGT GTCTGGTCAC TAGTGGACAA CACGACCAAG 
GAACGCACCG CTCACGCTTT CCTACAAGTT ACCGAAGAGG ACATTCAGAA ATTCAATAAC 
AGGATCCGTC AGATTCTCAT GTCGTCTGGC TCGACCACAT TCACCAAGAT TGCTAACAAG 
TGGAACACGG CGCTGATTGC CCTCTTTACT TACTACCGCG AGGCTGCTGT TTCGACCGTG 
AACCTGCTTG ACACGATTGT CAAGTGTGAA ACCAAAATCC AGACTCGTGT CAAAATTGGT 
CTCAACTCCA AGATGCCTTC CCGTTTCCCT CCTGCCGTTT TCTACACTCC CAAGGAGCTT 
GGTGGCTTGG GTATGATTTC CGGATCACAC ATCCTTATCC CTACTAGCGA CAAGCGCTGG 
TCTAAACAGA CAGATCTCGG AGTTACTCAT TACCGTGCGG GCATGTCGCA CGATGAGGAA 
ACGCTGATCC CCAACATCTT CCGCTACATC ATCCCGTGGG AAGCTGAGTT TATCGACTCC 
CAGCGTGTGT GGACGGAATA CTCGCAGAAG CGTCTCGAGG CCAACCAGCA GAACCGCAGA 
TTGACCCTAG AGGATCTGGA GGACAGCTGG GATCGCGGTC TGCCCCGTAT AAACACTCTT 
TTTCAAAAGG ACCGTAGCAC GTTAAGCTTC GACAAGGGTT TCCGTGCCCG TTCCGAGTTC 
AAGATCTATC AGTTGATGAA GAGCAATCCC TTCTGGTGGA CAAGCCAGAG GCATGACGGT 
AAACTTTGGA ACCTTAACGC ATACCGTACC GATGTCATCC AGGCGCTTGG TGGTGTCGAA 
ACCATCTTGG AGCATACCTT GTTCAAGGCA ACTGGTTTCC CGTCTTGGGA AGGTCTCTTC 
TGGGAGAAGG CCAGTGGTTT CGAGGAGTCG ATGAAGTTCA AGAAGTTGAC AAATGCCCAG 
AGGAGCGGTC TCAACCAGAT TCCTAATCGT CGTTTCACGC TTTGGTGGTC GCCTACCATC 
AACAGAGCCA ATGTCTACGT CGGTTTCCAA GTGCAGTTGG ATCTAACTGG TATCTTCTTG 
CACGGCAAGA TTCCTACTCT CAAGATCTCC CTGATCCAGA TCTTCCGAGC TCATCTGTGG 
CAGAAGATCC ACGAGTCAGT GGTCATGGAT CTTTGCCAGG TCTTCGATCA GGAGCTTGAG 
GCTCTAAGCA TCGAATCGGT TCAAAAGGAA ACCATTCATC CCAGAAAATC TTACAAGATG 
AACAGCTCCT GCGCGGATAT CCAGCTCTTT GCCAGCCACA AATGGAACGT TACTCGCCCA 
TCGCTCCTTT TCGATACCAA GGATGTCATC GAGTCTACTA CGACGAACAA GTTTTGGATT 
GATGTTCAGC TTCGCTATGG TGACTACGAC TCTCACGATA TTGAGAGATA CGTCAGGGCC 
AAGTATCTGG ACTACACCAC CGACAGCATG AGTCTTTACC CATCTCCTAC CGGTCTCATG 
ATTGGCATCG ATCTGGCTTA CAACTTGTAC TCTGCCTACG GTCAATACTT CCCAGGCTTG 
AAGCTCTTGA TCCAGCAGGC TATGGGCAAG ATCATGAAGG CAAACCCTGC GCTCTACGTT 
CTTCGTGAGC GTATCCGCAA GGGTCTGCAG CTCTACGCCT CCGAGAGCAA CCAGGAGTTC 
CTGAATTCGC AGAACTACTC GGAGCTGTTC AGCAACCAGA CTCAGCTCTT TATCGATGAT 
ACTAACGTCT ACCGTGTTAC CATCCACAAG ACCTTCGAAG GTAACTTGAC GACCAAGCCG 
ATCAACGGTG CCATCTTCAT CTTCAACCCG CGTACCGGTC AGTTGTTCTT GAAGATTATC 
CACACCAGCG TTTGGGCGGG TCAGAAGCGT TTGGGACAGT TGGCCAAGTG GAAGACTGCC 
GAAGAAGTCG CAGCCCTTAT CAGATCGCTA CCCGTCGAAG AGCAGCCAAA GCAACTGATC 
GTCACCAGAA AGGGTCTGTT GGATCCTTTG GAAGTCAATT TGCTTGATTT CCCCAACATC 
TCCATCCGCG CGTCCGAGTT GCAGCTTCCC TTCCAGGCCG CCATGAAGGT CGAAAAACTG 
GGTGACATGA TCTTGCGTGC CACCGAGCCG CAGATGGTCT TGTTCAATCT CTACGACGAG 
TGGCTGAAGA GCATCTCTTC CTACACTGCC TTCTCCCGTC TCATTCTCAT CCTGCGTGCC 
CTGCACGTTA ACCAGGATAA GACGAAGCTC ATCTTGCGCC CCGACAAGAC CGTCATCACG 
CAGGATCATC ACATCTGGCC CTCGCTCTCC GATGAAGACT GGATCAAGGT TGAAACGCAG 
CTCCGCGATC TTATCCTCAA CGACTACGGC AAGAAGAACA ATGTCAATGT GTCCAGTTTA 
ACTAGCAGTG AAGTCCGTGA TATCATTCTT GGTATGGAGA TCTCAGCCCC CTCGCTGCAG 
AGGCAGCAGG CGGCGGAGAT TGAGAAGCAG CAGCAAGAGC AGCAGCAGCT CACCGCCGTC 
ACTACCAAGA CGCAGAACGT GCACGGCGAG GAGATCATTG TGACGACCAC TTCGCAGTTC 
GAACAGCAGA CGTTCGCCTC CAAGACCGAG TGGCGCACCA GAGCCATCGC CACCTCGAAC 
CTACGCACCA GGGCTAACAA CATGTACGTG TCGCCCGTGG ATAGTGACCT GGACGACGTG 
ACCTATGTCA TGCCCAAGAA CATCCTCAAG CGCTTCATCG CCATTGCCGA TCTCCGTGTC 
CAAGTCGCCG GTTACCTCTA CGGTGCCTCG CCCGCCGACA ACGACCAGGT CAAGGAGATC 
AAGTGCATCG TCATGGTGCC TCAGATCGGT GGTCTGCGCA ACGTTCAGTT GCCTCACCAG 
CTTCCCCAGC ACGAGTACCT CAAGGACATG GAGCCACTCG GCATCATTCA CACACAGTCG 
GGCAACGAGC TGCCGTACAT GTCCGCCATG GACGTTACGG AGCACGCACG CCTGCTTGAT 
GCCCACCCGA ATTGGGACAA GCAAAACACA CTCACCGTCG CCGTCTCCTT TACGCCCGGT 
AGCGTGTCGC TGTCGGCCTG GGCGCTCACG CCTCAGGGCT TCAAGTGGGG CGTGGAGAAC 
AAGGACATAG CCAGCGACCA GCCGCAGGGG TTCACAACCA GCATGGGCGA AAAGCGCCAG 
CTCCTCCTCA GCGAGAAGTT CCGGGGCTTT TTCCTGGTGC CGGATGGCGG CAAGTGGAAC 
TACAGCTTCA TGGGCAGCGC GTTTGGAGGC CTGGAGAAAA AGCCGGTGCA TGTCAAGCTG 
GATACGCCGC TGCCCTTTTA CAGCGACCAG CACAGACCTA TTCACTTCTC GAGCTTCAAC 
GAGTTAGAGG ATATCTGGGT TGATAGGCAG GATAACTTTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU07832 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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