sad-3 cDNA ORF clone, Neurospora crassa OR74A

The following sad-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sad-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf10954 XM_954062.2
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Neurospora crassa OR74A suppressor of ascus dominance-3 (sad-3), protein, partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf10954
    Clone ID Related Accession (Same CDS sequence) XM_954062.2
    Accession Version XM_954062.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product suppressor of ascus dominance-3 protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_954062.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAACC GCGACGAGTG GATTCAACTG AGCGCTACGA GAGACGAGAT CGTCCGTAGC 
    AATATTTTTG TCAAGCTTGA AGATGGAGAC GGATGGAAGT CGAAACCCGA AATTCCCACG
    GCCAAGGAAA TCCTTCTACT CCAGAAAGAG GCCGAAGGTC TGCCTGAGAA TGATGTAAGG
    CAGCCCTGGA AATCCAAAGA GGACTACTTG AAGGCTCAGT ACGAGATCCT TCGTCGCGAG
    GGCACTGAAG GTTTGCGATT CGCTGTCAAC AACTACATCT ACAACAACAA CCACGGACTG
    CGCCAGGATG ACGATCAGCA CATGTTCGTT TATACCCAGG TTCGCATCAA GTCGTACATC
    ATGACAACAT TAGGACCTCT TGCGCGTGTT GAGTTCATGC CGCAGAGAGC CATCAAATGG
    CTGAAGTCCG GTCGCCTAAA ACCCGGCAGT CTTCTTGCCC TCACCACCAA AGCCGACAAG
    TTCAAGACCA TCTGCAAGGT CGCCGTGGTT GCCCAAAGGC CGTACTTGCT TGGACTTGAT
    CAAACCCCTC CACTCGTCGA CCTCATGTGG GGCAACCCAG AAGATGCCGT CTTCGATTGT
    GATCTTGAAC TTGTTATGAT TGAGGCCCAA CACGGCTATT TTGAATCCTC GCGTCATTCT
    TTGGTCGGCC TGCAACTGGC TGCGCAGGCA GATACTCCTA TCGACAAATA CATCACCGGC
    AGTCACAGGG GAAATGAGAC ACCAAGCTTC ATATACCAAA GCCCTATTAT GGACTTCTCT
    TCTGTTGTGC GCCAACCCCT TGCAGACCTT GAGAAGCTCA AATCCCTGCG CGAGTTCAAT
    GTGCTCTCTG GACTCCTCCC AAAAGATGTG GAAAGTATCA GCTTTCTCGA CGCCTCGCAA
    CTATCCGCTC TTCATCGAAT GGTCTCCAAG GAGCTTGCCA TCATCCAAGG CCCTCCAGGA
    ACGGGGAAAA CTTTTACCTC GGTGGAGGCA ATCAAAGTCA TGCTTGCGAG TCGCCGCAAG
    TGTCCAGGAC ACAACCCGCC CCTCATTGTC GCTGCGCAGA CAAATCATGC CTTGGACCAG
    CTGCTTGGCC ATTGTCTTGA GGCGAACGCA AAGATTCTGC GTCTTGGCTC AAGAAGTGAG
    AGAGAAGACA TGAGGCCTTA CAGCTTGTAT GAGATGCGCC GCAAATGCTC CCGTGGTGGG
    GGTGGAAAGC GGTTCAGGGA ACTGGAGGAT GACCGTCATA CCAACATCGA CAGAGTAGAG
    GCACTGGTTG ACAGTGTGTT TGGGGACAGT CTCATCGACC CACGCCAGCT TCATGCACAT
    GGCATCATTA CCGAAGCGCA GCTGGAGTCT TTGTACGATG ATGAAATGGA GACAAGCCCG
    GAGTTGGAGA AGCTTGGTCC CTTTTCCTTA TGGTTGGGAG ACTCACGAAT CCCGGCTCGG
    ATCGTTCGCG ACAGGCAGCC TCTCATATCC GAAACATTGG AAGCTGACGA GGACTTCGAT
    TATGAGCGCG ACGTAGAGAA CATCGCGTAC GATGAAGAAG ACGCAGACAG GATAAACGGC
    AAGGAAATCA AATTGATGCA TATATGGACC GGAAAAGAGC CTGGACACTT CAAAAGCTGG
    AATCATCGTG CACAACGCTT GCTCAGAGAG AATGATAACC TCTTTGATAT ACCCCAAGAC
    TGGAGGGGTG CAGTTTACCA GCATCTCCAG TCCAAACTGG TGCAAGCAAC CACTCCGACG
    CTGGCTGGGC TGCTGGCCAA ATACGTTGAG ATATGCAAGC GGCGCAGAGC ACTCAAAGCC
    CGGCAAGATA TTGACATGGC CTTCTCACAA GATATTGCGG TCATCGGCTG CACAACAACC
    GGCCTTACAA AATACCGCGC GTTTCTGGCC GGGCTACAGC CCAATACCCT GCTTATCGAA
    GAAGCTGCCG AAACAAGGGA AGGAAACATT ACCTCGGCTC TGTACCCTTC CCTTCAACAG
    CTCGTTCTGG TTGGTGACCA TGCACAGATG TCGCCTCGAT GCGACATTCG ATGGCTCGGT
    CAACATCCGT ACAACTTGAA CGTCTCTCTG TTTGAAAGGC TCATCAACCT GAAAATGAAT
    CACATCATGC TCAATCAACA GCGCCGCATG AGGCCGGAGC TGAGACGCAT AGTCTCTCCA
    TTCTATAATA ACCTACTCGA CCACCCCAGT GTCCAATCTC CGCAAGCACG TCCGGATGTC
    CCTGGGATGG GTGGGCGTAA TTGCTGGTTT TTCGATCACG AATGGATTGA GCGAACGACT
    TCTGACAACA GCAAACTCAA CGACCAAGAG GCTGAAATGA TCACCCTCTT CTTTGTGTAT
    CTTGTGTCTA ATGGTGTGTC TAGTGAGAAG ATAACCATCT TGACATACTA CAGGGGCCAA
    CGAAGTCTTC TCTTGAGAAG ACTAAAAGGG CATCCATCGC TCACTGGCTG TTACTTCAAC
    GTTTTCACAG TGGACTCGTA CCAGGGTGAA GAAAATGATA TTGTTCTCTT GTCCCTGGTG
    CGTAGCCCTG ACCCAGTCTA TGGGCGCAAC ATCGGTTTTC TTGACAACCC ACACCGAGCT
    GTGGTTGCTA TCAGTCGCGC TCGGCAAGGG TTCTACATAT TCGGCAACGT CAATAACTTA
    CTCGAGGCTA AAACCGAATC ATCGTTTCTA TGGGGCCTTA TTTTCAATAG ATTCGCTGAT
    CAGGGCATGG TCAACCGAGA ACTTGGGCTT CCTCTGGTTT GCCAGAATCA TGGAAATGAA
    ATCTGGATCA AGGAGCTAGA GGACTGGGCT GACAATACTG CAGCCCATGT TTTCAAATCG
    TTCCTGAATG TGGGCATCCT TGTCACGGCC TTTGCGGTGA AAGATGTTTC CACGACTGGC
    AAGACCATCG AATCTCTTTT ACTCCAGCAT GGCGCTCACG ACTTTGGGCA TGAGGAGCCA
    GTCAAGCGCA AAAGTATTGT ATCGACGGGA AGTACTGGTC GTGGGCTTTA TAGTCATTTC
    GGGGAGCTGC CATTGCGTCC TATTGGTGAC AATTGGAAGA ATGCCGAAGC CGTGATCCAG
    CAACAGGACA GAAAGCTTGA CAAAGAACTT GCAGAGAGGA ATGAGAAGTG GCTCAGCCAG
    ACTCATCATG TGCAAGATCA ATGGATGCCA ACAGGCGTCA CGGCTTCCGG AACTCGTATT
    CGTGCTGGTC CAATAATCAC TCAAACCCTC CAGCTTCTAA GCGACTTGCC GGCCATGACC
    ACCACAGCCG CTGTGACGGC TGGTTCTGGG AGCCATTTGC GTACTGGCTC TGGTGCTGGT
    CCAATCTTTG CGCCCACACT GAATCAGTTC CCCAACACCG CCCCTATCGC TCAGCCTGAG
    CTTCTAATTC AACTAGAGCA AGACGACTAT GCCGTCTCTG CGTCTAATTT GAAACAGACA
    ATAGGAGATT TGGAGAATGA TGGAGAAGAG AACTGGTTGA TTGACTTTGG GGAACCCGAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_959155.2
    CDS1..3483
    Translation

    Target ORF information:

    RefSeq Version XM_954062.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A suppressor of ascus dominance-3 (sad-3), protein, partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_954062.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAACC GCGACGAGTG GATTCAACTG AGCGCTACGA GAGACGAGAT CGTCCGTAGC 
    AATATTTTTG TCAAGCTTGA AGATGGAGAC GGATGGAAGT CGAAACCCGA AATTCCCACG
    GCCAAGGAAA TCCTTCTACT CCAGAAAGAG GCCGAAGGTC TGCCTGAGAA TGATGTAAGG
    CAGCCCTGGA AATCCAAAGA GGACTACTTG AAGGCTCAGT ACGAGATCCT TCGTCGCGAG
    GGCACTGAAG GTTTGCGATT CGCTGTCAAC AACTACATCT ACAACAACAA CCACGGACTG
    CGCCAGGATG ACGATCAGCA CATGTTCGTT TATACCCAGG TTCGCATCAA GTCGTACATC
    ATGACAACAT TAGGACCTCT TGCGCGTGTT GAGTTCATGC CGCAGAGAGC CATCAAATGG
    CTGAAGTCCG GTCGCCTAAA ACCCGGCAGT CTTCTTGCCC TCACCACCAA AGCCGACAAG
    TTCAAGACCA TCTGCAAGGT CGCCGTGGTT GCCCAAAGGC CGTACTTGCT TGGACTTGAT
    CAAACCCCTC CACTCGTCGA CCTCATGTGG GGCAACCCAG AAGATGCCGT CTTCGATTGT
    GATCTTGAAC TTGTTATGAT TGAGGCCCAA CACGGCTATT TTGAATCCTC GCGTCATTCT
    TTGGTCGGCC TGCAACTGGC TGCGCAGGCA GATACTCCTA TCGACAAATA CATCACCGGC
    AGTCACAGGG GAAATGAGAC ACCAAGCTTC ATATACCAAA GCCCTATTAT GGACTTCTCT
    TCTGTTGTGC GCCAACCCCT TGCAGACCTT GAGAAGCTCA AATCCCTGCG CGAGTTCAAT
    GTGCTCTCTG GACTCCTCCC AAAAGATGTG GAAAGTATCA GCTTTCTCGA CGCCTCGCAA
    CTATCCGCTC TTCATCGAAT GGTCTCCAAG GAGCTTGCCA TCATCCAAGG CCCTCCAGGA
    ACGGGGAAAA CTTTTACCTC GGTGGAGGCA ATCAAAGTCA TGCTTGCGAG TCGCCGCAAG
    TGTCCAGGAC ACAACCCGCC CCTCATTGTC GCTGCGCAGA CAAATCATGC CTTGGACCAG
    CTGCTTGGCC ATTGTCTTGA GGCGAACGCA AAGATTCTGC GTCTTGGCTC AAGAAGTGAG
    AGAGAAGACA TGAGGCCTTA CAGCTTGTAT GAGATGCGCC GCAAATGCTC CCGTGGTGGG
    GGTGGAAAGC GGTTCAGGGA ACTGGAGGAT GACCGTCATA CCAACATCGA CAGAGTAGAG
    GCACTGGTTG ACAGTGTGTT TGGGGACAGT CTCATCGACC CACGCCAGCT TCATGCACAT
    GGCATCATTA CCGAAGCGCA GCTGGAGTCT TTGTACGATG ATGAAATGGA GACAAGCCCG
    GAGTTGGAGA AGCTTGGTCC CTTTTCCTTA TGGTTGGGAG ACTCACGAAT CCCGGCTCGG
    ATCGTTCGCG ACAGGCAGCC TCTCATATCC GAAACATTGG AAGCTGACGA GGACTTCGAT
    TATGAGCGCG ACGTAGAGAA CATCGCGTAC GATGAAGAAG ACGCAGACAG GATAAACGGC
    AAGGAAATCA AATTGATGCA TATATGGACC GGAAAAGAGC CTGGACACTT CAAAAGCTGG
    AATCATCGTG CACAACGCTT GCTCAGAGAG AATGATAACC TCTTTGATAT ACCCCAAGAC
    TGGAGGGGTG CAGTTTACCA GCATCTCCAG TCCAAACTGG TGCAAGCAAC CACTCCGACG
    CTGGCTGGGC TGCTGGCCAA ATACGTTGAG ATATGCAAGC GGCGCAGAGC ACTCAAAGCC
    CGGCAAGATA TTGACATGGC CTTCTCACAA GATATTGCGG TCATCGGCTG CACAACAACC
    GGCCTTACAA AATACCGCGC GTTTCTGGCC GGGCTACAGC CCAATACCCT GCTTATCGAA
    GAAGCTGCCG AAACAAGGGA AGGAAACATT ACCTCGGCTC TGTACCCTTC CCTTCAACAG
    CTCGTTCTGG TTGGTGACCA TGCACAGATG TCGCCTCGAT GCGACATTCG ATGGCTCGGT
    CAACATCCGT ACAACTTGAA CGTCTCTCTG TTTGAAAGGC TCATCAACCT GAAAATGAAT
    CACATCATGC TCAATCAACA GCGCCGCATG AGGCCGGAGC TGAGACGCAT AGTCTCTCCA
    TTCTATAATA ACCTACTCGA CCACCCCAGT GTCCAATCTC CGCAAGCACG TCCGGATGTC
    CCTGGGATGG GTGGGCGTAA TTGCTGGTTT TTCGATCACG AATGGATTGA GCGAACGACT
    TCTGACAACA GCAAACTCAA CGACCAAGAG GCTGAAATGA TCACCCTCTT CTTTGTGTAT
    CTTGTGTCTA ATGGTGTGTC TAGTGAGAAG ATAACCATCT TGACATACTA CAGGGGCCAA
    CGAAGTCTTC TCTTGAGAAG ACTAAAAGGG CATCCATCGC TCACTGGCTG TTACTTCAAC
    GTTTTCACAG TGGACTCGTA CCAGGGTGAA GAAAATGATA TTGTTCTCTT GTCCCTGGTG
    CGTAGCCCTG ACCCAGTCTA TGGGCGCAAC ATCGGTTTTC TTGACAACCC ACACCGAGCT
    GTGGTTGCTA TCAGTCGCGC TCGGCAAGGG TTCTACATAT TCGGCAACGT CAATAACTTA
    CTCGAGGCTA AAACCGAATC ATCGTTTCTA TGGGGCCTTA TTTTCAATAG ATTCGCTGAT
    CAGGGCATGG TCAACCGAGA ACTTGGGCTT CCTCTGGTTT GCCAGAATCA TGGAAATGAA
    ATCTGGATCA AGGAGCTAGA GGACTGGGCT GACAATACTG CAGCCCATGT TTTCAAATCG
    TTCCTGAATG TGGGCATCCT TGTCACGGCC TTTGCGGTGA AAGATGTTTC CACGACTGGC
    AAGACCATCG AATCTCTTTT ACTCCAGCAT GGCGCTCACG ACTTTGGGCA TGAGGAGCCA
    GTCAAGCGCA AAAGTATTGT ATCGACGGGA AGTACTGGTC GTGGGCTTTA TAGTCATTTC
    GGGGAGCTGC CATTGCGTCC TATTGGTGAC AATTGGAAGA ATGCCGAAGC CGTGATCCAG
    CAACAGGACA GAAAGCTTGA CAAAGAACTT GCAGAGAGGA ATGAGAAGTG GCTCAGCCAG
    ACTCATCATG TGCAAGATCA ATGGATGCCA ACAGGCGTCA CGGCTTCCGG AACTCGTATT
    CGTGCTGGTC CAATAATCAC TCAAACCCTC CAGCTTCTAA GCGACTTGCC GGCCATGACC
    ACCACAGCCG CTGTGACGGC TGGTTCTGGG AGCCATTTGC GTACTGGCTC TGGTGCTGGT
    CCAATCTTTG CGCCCACACT GAATCAGTTC CCCAACACCG CCCCTATCGC TCAGCCTGAG
    CTTCTAATTC AACTAGAGCA AGACGACTAT GCCGTCTCTG CGTCTAATTT GAAACAGACA
    ATAGGAGATT TGGAGAATGA TGGAGAAGAG AACTGGTTGA TTGACTTTGG GGAACCCGAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B