NCU08993 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU08993
Entrez Gene ID	3875178
Full Name	HEAT repeat containing protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_953928.2	XP_959021.1	HEAT repeat containing protein

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_04982	XP_003712481.1
Schizosaccharomyces pombe (fission yeast)	sip1	NP_595540.1
Neurospora crassa	NCU08993	XP_959021.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU08993 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU08993 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf00956	XM_953928.2 Latest version!	Neurospora crassa OR74A HEAT repeat containing protein (NCU08993), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU08993 ( XM_953928.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_959021.1 Neurospora crassa OR74A HEAT repeat containing protein (NCU08993), mRNA.

Price & Availability↑

CloneID	ONf00956
Clone ID Related Accession (Same CDS sequence)	XM_953928.2
Accession Version	XM_953928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	HEAT repeat containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_953928.1.

ATGTCATCCA CACCAGCTGA CGCCACCAAT GGCGAGCGCA ACGGCATCAC GAACACACTG 
ACATCCCACG CTACGACGAA TCCCGAGCTC GATCTTGCCA AGCTTCAAGC GCTCCCCGCC 
GAGCAGCAGG AGCTGTTCCT CCTGACGTCC GTCTCGGCAC TTAGCAGGCA TGTCCTGACG 
CTCGAACCAG ATGACTGCAC CGCGCAGCAG TTCTACATAA AAAAGGAGGC CTTCAAGGTC 
CTGAATCTTG CCTCGCCATC GCCAACAAGA GTCATACGAA ACGCGCTGGG GCGGTGTCTT 
GCGCACGTGT TCGACAAGGG CGATAGGAAG CTCCTGTTCG AAACTGTTAA CGAGCTGGTC 
GGCATCATCT CGAGTGGGAA ATTGAAGACG GAGGGTGAGA TACGGACAAA ACATGCTGCC 
GTGGCTTGCT TGGGGGATAT ATACGGCTCG GCTGGTGACA GCGCCATTGG CTTGCACCAG 
CTAGTATGCT CGGCGCTCTT GAAGCTATTG AAGGCAGCTT CGAACCACGC AGGGCTTCGT 
GCAGCTGTCC TGACCGCTTT CGGGAAGATT GTCACCATGA TACAGGGGAG CATGGACGAG 
AATGTTGCGA GAGATATATG GAAGCAGGGG AGAAACCATG CCTCTGGAGA CAAAGGATCA 
CTGGTCGTCG TGGCCGCTTG CCGATGCCTA CGGTCGCTTG TGCAGTATAC CCCCTATTTC 
AAGAACTCGA CCGACTTCGA CAAGCTCAAA TCGACCTTGT TCAAGACGTT CGACAATCCC 
TCATCAAACG CGCGATCGGC AGCAGCGGAC TGCTTTGCCC AAGCGCTGGT CGGGGGTTAC 
TCCGAGTCAG CCATTGGCGA GGCACCGCTG CCCCTGGTCA AGAAATCCAA GTCCAAGGCG 
ATCAAGAGAC AGTCAACAAT GGGAGGCCTT CAAGATGACG AGGAGGTTCC CTCACGGCCC 
GAGAGCCCTG CTCCAACCCA GAGACCCCAA GATTTGACCT TGTCGCTGGC CAATATACTC 
AAAACTTTGT CCACCCAATA CGTCCGCATT TCGACCACCA ATAAGGCAAG GGCTGCCATC 
GCTATTTGTA TCGGAAACGT TCTCCAGAAG CTCGGGGAAA AGACTGTAGA AGTCAACTAT 
CTTTGCATTC TCGAGATCCT TACAGCTGAC TTCTTGGGCC ATAACAACAT TCTCAACAAC 
CGGTACCGCT TACTCATTTC GCGGCGCATG GTCGATACCA TAATCCAGGA CATATTAGGA 
AAAAGGATCC TGGGTGAATC GGGGCAAATC ACGGCTGCTA AAGCTATCGT CAACGATATC 
CTCAAGAACT ATCCTCAGGC GCTCAAGGAA AAGCCGGAGC CATCCAAACA CACATTGGTT 
GTCTCACTGA GTGCTCTGTC CTCGCTGATC TCCAGTTTGG GCACAGCGGT GACCGACTTC 
TCAGAGGGAT GTCGCGATGG CCTGCTTCAA GTTCTCCAGC ACCCTAGCTA TACCGTTCAG 
GTATACGCCG CGGCCTGCAT GAAGAATTTT GTGTTGGCCT GCCCGCAACA GCTTCTTCCC 
TGTTTGTCAG TTTGCATGAA CAGCTTGAGC CGAGAACTGA GCCTTCTTGG GACGGGAAGA 
AATTCCCCTC GCAGGTGCAT TGGTTTCGCC CACGGCCTCG CTGCCGGGCT CAGCGCAAGC 
CCGCAACGCC CGCTCTATGG GTCGGTCGAG ATCAACAGTC GTGTTCTGAC CATGGCTACA 
AACTTGCTCA AGTCAAGTGG AACTTCGGAA CTACGGGTTG CAAGCACCCA GATCCAAGTT 
GCCTGGACCT TGATAGGCGG CCTAATGTCT CTTGGTCCCA ACTTCGTCAA GATTCATCTC 
TCGCAGCTGC TTTTACTCTG GAAGAATGCG CTGCCCAAGG TTCTGGCAAA GGATAGCATG 
GCGGTTCATA GGAATCTGCT CAATGCCTCG TTCCTCACAC ATGTCAGGGA ATGTGCGCTT 
GGGTCTATCC TAGCGTTCCT TCAGTTCAAC AGTCGCCTCT TGACTGTGGA CGTATGTAAG 
AGAATTGCTA CGATGTTGCA GAGTACTACT GCATTTCTCA AGACACTACC AGCGAAGAAG 
ACATCGGATG ATATAAGCGA GAGGCTGACT CCAGCCTTGC AACTCCAGGA TCTGGAGGTT 
ATGGTACGAC GCAGAGTCCT TCAGTGCTAT ACGAAGCTCG TGAACGTTAG CCCTGCTGGC 
GCCACTGAAG CACTCCTGCA GTCCAACCTT TTGACTCTCG CCATCTCTCT CTTTGCCGAC 
CCGGATAACT ATACACCGAG CTCCCTCAGC ACTTCCATCG CGAACGCCGC AGGTACCTTC 
GACTCTATTT GGGAGGTGGG CGACAATTCA GGGTTTGGAG TGACCAGTCT TGTTAGGGGC 
TTTGCGACAA GAAGCCTCCC GGGACAGCAG ACCAACGGCT CAGACAGCAC CCAACAAGAG 
TACGAACCGG AGGAGTGCAT CGAGAGGCTG CTTCTTTCTC CCGTATGCGG CACCCTGGAG 
AACGACGCTT GTCTTCTGTA CATCGGTAAC TCGGATAACC CGGGACTTCC TGATCCTGCT 
GCCACCGAGG TGGTTAACAT GGCTATCCAG TTGTTTGCCT TTGTCTTCCC TTTGACCCCA 
TCTAAGGTGC AGGAGAGTGC CTTGGAGCAA ATCACGACCT TCATAAGGGC TGGCTCCCTT 
CAGCGGGAAA CTGGCCGCAA GGCTGCTGTT GATGTCAACG TGGTTACGGC ACTTCTGTCT 
ACCCTCCGCG TGGCTTGCAA GGAGACCAAG TCGTCACCAG GCGACATCAC AAACATGGCC 
GTAGAGAAAC TTCTACAGGA GCTACTGAGA GATTTCGTAC TTGACCCCGA TCAGTACGTC 
CGTAGCATTG CTTACGAGGC AATTGCCAGA CTTTGCAGCG CTTGTGGCAA CGCATTCACG 
AATCAAGAGA TCAAGTTCCT TGTTGATACT ATTGTTGTCA ACCGAGAACC TAGCGCCAGA 
GCGGGCTGCG CCATGGCTCT TGGGTGCATC CAGACCAAGA TTGGCAGCAT GGCGGCTGGG 
TATCATTTGA AGACGATTCT TAACATCCTC ATGTCTCTGT GCAACGATCC GCACCCGACT 
GTTCATTACT GGGCTTTGGA GGCTATAGCA CGCGTATCAG ATGCTGCTGG ACTCGGCTTC 
TCCAGTTTCG TGTCTAGCAC ACTAGGAATG TTGGCCAAGC TCTACGTCTC GGATACGCAT 
AACCCTGAAT TAGCTTCTCC GATCACCATG AACTTGGAGG CTGACCTCTC CTCATCGGCT 
GCGATTGCTC GCTGCGTTGA TGCGCTCATC AACGTCCTTG GTCCAGATCT ACAAGACTCT 
ACGAAGTCTA GGGAGCTGAT CCTTACTCTT GTTGGGTATT TCCAGCAAGA AGAGGATCTT 
GAGATTCAGC GGGCAAGTCT CGCTTGCATG GAACATCTGA CATTGTATGC TCCGGGCTAC 
ATGGACTTCA TCGACTACGT CAGGACGCTT CAGCGATACA TCAAGTCCGA TCATGCCGCC 
CTCCGAGATG TGGCCGTGGA CAGTCTTTAT AATCTCATGA AGAGGAACTC GTACGATGTC 
ATCAAGGCCG CCGAGCAAGG CTTCGAGGAT CATCTGTGGC TGGTTCTCGA TGCCTCGCCG 
AGTCATGACG GAATGCGCAA CCTTATCAGA AACTGGCTCC GCCAGACCTG TCTTTCGGAC 
ACAGCCGCTT GGCTTGCGCG CTTCCAGCAT GTCCTGAAGA TGACCAGACC GAAAGCCTCC 
GCGCAGGCAA CCACGAACAC CGGGAGGTCT GGTGGAGGCA TTGACCTGCA GGACGAAGAG 
GTAGCTGGCT TCGCCGCTGC TGGAGGAGGC GCCAAGGACG ACAAAGATAC GCCGAGCGGC 
TCAGATGTGG AGCCCCTGAA GTGGCAGGTC ACTACCTTCG CCATGGACTG CCTGAACGAT 
ATCTTCATTT TGGTTACAAA AGATGTCGCA ACTCATGGCG AATCTGCTGC GCAGGCGGCC 
CTTCAGAACA AGGTTGCTGA TGTCGTTCGC ATGGCGTTCT CGGCGTCCAC GTCAGGTGTC 
TTGGACCAGA GAATCTGGGG CTTGAAGATC ATTGGCGCTG TTCTCAAAAT GTTTGGCAAG 
ATTCCAGACC CAGACTTTGA GGAGGCCATG CTGCTTGAGC AGTACCAAGC GCAAATCAGT 
TCCGCTCTTA CGCCCGCTTT TGCCGCGGAC TCGTCACCCG AACTGGCCTC GGAAGCCGTC 
GATGTTTGTG CAAGCTTCAT CGCCACAGGC ATTGTCACTG ACGTAGACAG GATGGGTAGA 
ATTCTGAAGA CGTTAGTGAG CTCGTTGGAG AACTTTGCAA AGGATGAGGA TAATGCTGGC 
ATTGGTGACC TCAAGGGACT CAGCTCAAAC GCACAGGTTA TGGTCAAGAT GTCTGTCTTC 
GCCGCCTGGG CCGAGCTACA AGTCGCCAGC TCTGAACAAA AGTACCTGCT GGATGTTCTC 
AAGCCTCATA TTGGTACCTT GACTCCTCTT TGGCTCGAGT CGTTACGTGA ATTTGCTCGG 
CTACGGTTCG AGCCGGATAT TTCCATGACG CTCGGCCCTC CATCGCTTTC TGGAAGCTTG 
GACACTGTCT ATGCAGCGCT GAACCGTGAG ACGTTGCTCA AGTTCTACCA GGAGTCTTGG 
TTGAAGTTAG TAGACGCTAT CGCCAGCCTC ATTGAACAGG ACAGCGAGTT CGTCTTTGAT 
GCCCTCGATG GCAAGGAGCT GTCAGGACCT ACCACCAACA GCAGCTCAAA GGGGGCAGAT 
ATCAACTACC GCGATGAGCC TGTTGCGTTC TTCTTTGTCT TGTTTGGTAT CGCGTTTGAA 
GCGCTCGCCA CGAGACCAGG CCAGAGCGAG TCTCTCGCTA CCCGAGAACA AACACTGGAG 
ATTCTCCGCG CTCTTAAGAA GATCCTTCAC CCAAGCGTAT CTGGTCATGC CATTTACCGC 
GATGCCATCT TCTCCGAGAC GATGGATCTG CTGGACCGTC TCGTACTCAC CGAAGGTCTT 
GATGTGCAGG GCGTTATCGT TGAGATTGCG CGTGCCCTAT GTGTGGCACA TCCAGCAGCC 
AGAAAGCAGG CTGACGACCC CGAGAACAGT GAGTTGTCTG ATGACATTGA GCAGCTCTTC 
GAGCTCACTC GGATCATCGT CTTGGTCCTC TCCGGACTGT TGCCCAATCT CAGTGAGACC 
CAACAGCCCG TGCGCCACCA GATGACAGAG GAAGCGATCC TTCTCGTCAA GACATCGCTC 
AACGCACTGG TCGATGCCGC GGAGGTGTTC CCATCCATAA TCAAGACGGA TCTACACGCC 
TGTATCCTGC ACATCTTTGC AACCATTCTT GCCACACCGT CATGCCAGGA GGTCATTGTC 
CCCCAAAACC TGTCGACACT CAAACGCTTT ATCAACGGCA TGACCGGTTC CAGGAGAGAC 
GGCGGAGATG ATGACGAGGG TAATTCGCCC ACGGACGTTC AGCTGCTGGG ATGTCTGCGC 
CGCTTCCTGT CCATCTATCT CAACGCTCAG AAGCGCGAAG CCCCGACGTC CTTGACCTGC 
GTCAAGAACT GTCTCCTGGC CATCACTATC CTCTTCACTG GTGGCAAAAA CCACCTGCCA 
GCCAGCGAGC CGCTGGTAGC CAAGTTCCTA GACGAGCTCG TAGACTGTCT AACTGACCGG 
ATGACCGCCA AAATAGCCGC AAACTGCATC CGCTCCCTCC TCCTCAACAC CAACGCCAAC 
AACCAACCCA CCTCAGCCGA TCACTCTATC TCCCGCCACC TCCTCCCGCG CCTCATTGCC 
TTCGTGACCA ACACCTCTCC TGAGGACCCG GAACAAGCTC GTTCGCTCAT CGCCCAAACC 
CTCGTGCAAT TTACCGCCGT GGTTGCCAGA GGATCGTCGC AGGACGGAGG CCAAGGCGCA 
TCCATTGCCA TGGCCCTCGT TGTGCCGACT TTGCTCTCGC GCGCGGCGAC AGAGGGAGCT 
AAAGATGATG CATACCGCGA GACGAGTGCA AGGCTACTGG AGTTGGCGAG CGCCGATCAG 
ACTGCTTTTA GGGCGGTGGT GGGCGCGATG AGTGAGGGCC AGAGGGCGTT TATGGAGGAG 
GTTATCAAGA GTGGACGATC GGGGGGAATG GGTGGTGGTG GTGGTGGCGC ACATGTAGGT 
GCCGGAGGGA GGAGAAGTGA GGAGGACGAA GAGCAGGCGC CGACTATTGC TTTGAAGATG 
AACTTTGGTG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_959021.1
CDS	340..6594
Translation	MSSTPADATNGERNGITNTLTSHATTNPELDLAKLQALPAEQQELFLLTSVSALSRHVLTLEPDDCTAQQFYIKKEAFKVLNLASPSPTRVIRNALGRCLAHVFDKGDRKLLFETVNELVGIISSGKLKTEGEIRTKHAAVACLGDIYGSAGDSAIGLHQLVCSALLKLLKAASNHAGLRAAVLTAFGKIVTMIQGSMDENVARDIWKQGRNHASGDKGSLVVVAACRCLRSLVQYTPYFKNSTDFDKLKSTLFKTFDNPSSNARSAAADCFAQALVGGYSESAIGEAPLPLVKKSKSKAIKRQSTMGGLQDDEEVPSRPESPAPTQRPQDLTLSLANILKTLSTQYVRISTTNKARAAIAICIGNVLQKLGEKTVEVNYLCILEILTADFLGHNNILNNRYRLLISRRMVDTIIQDILGKRILGESGQITAAKAIVNDILKNYPQALKEKPEPSKHTLVVSLSALSSLISSLGTAVTDFSEGCRDGLLQVLQHPSYTVQVYAAACMKNFVLACPQQLLPCLSVCMNSLSRELSLLGTGRNSPRRCIGFAHGLAAGLSASPQRPLYGSVEINSRVLTMATNLLKSSGTSELRVASTQIQVAWTLIGGLMSLGPNFVKIHLSQLLLLWKNALPKVLAKDSMAVHRNLLNASFLTHVRECALGSILAFLQFNSRLLTVDVCKRIATMLQSTTAFLKTLPAKKTSDDISERLTPALQLQDLEVMVRRRVLQCYTKLVNVSPAGATEALLQSNLLTLAISLFADPDNYTPSSLSTSIANAAGTFDSIWEVGDNSGFGVTSLVRGFATRSLPGQQTNGSDSTQQEYEPEECIERLLLSPVCGTLENDACLLYIGNSDNPGLPDPAATEVVNMAIQLFAFVFPLTPSKVQESALEQITTFIRAGSLQRETGRKAAVDVNVVTALLSTLRVACKETKSSPGDITNMAVEKLLQELLRDFVLDPDQYVRSIAYEAIARLCSACGNAFTNQEIKFLVDTIVVNREPSARAGCAMALGCIQTKIGSMAAGYHLKTILNILMSLCNDPHPTVHYWALEAIARVSDAAGLGFSSFVSSTLGMLAKLYVSDTHNPELASPITMNLEADLSSSAAIARCVDALINVLGPDLQDSTKSRELILTLVGYFQQEEDLEIQRASLACMEHLTLYAPGYMDFIDYVRTLQRYIKSDHAALRDVAVDSLYNLMKRNSYDVIKAAEQGFEDHLWLVLDASPSHDGMRNLIRNWLRQTCLSDTAAWLARFQHVLKMTRPKASAQATTNTGRSGGGIDLQDEEVAGFAAAGGGAKDDKDTPSGSDVEPLKWQVTTFAMDCLNDIFILVTKDVATHGESAAQAALQNKVADVVRMAFSASTSGVLDQRIWGLKIIGAVLKMFGKIPDPDFEEAMLLEQYQAQISSALTPAFAADSSPELASEAVDVCASFIATGIVTDVDRMGRILKTLVSSLENFAKDEDNAGIGDLKGLSSNAQVMVKMSVFAAWAELQVASSEQKYLLDVLKPHIGTLTPLWLESLREFARLRFEPDISMTLGPPSLSGSLDTVYAALNRETLLKFYQESWLKLVDAIASLIEQDSEFVFDALDGKELSGPTTNSSSKGADINYRDEPVAFFFVLFGIAFEALATRPGQSESLATREQTLEILRALKKILHPSVSGHAIYRDAIFSETMDLLDRLVLTEGLDVQGVIVEIARALCVAHPAARKQADDPENSELSDDIEQLFELTRIIVLVLSGLLPNLSETQQPVRHQMTEEAILLVKTSLNALVDAAEVFPSIIKTDLHACILHIFATILATPSCQEVIVPQNLSTLKRFINGMTGSRRDGGDDDEGNSPTDVQLLGCLRRFLSIYLNAQKREAPTSLTCVKNCLLAITILFTGGKNHLPASEPLVAKFLDELVDCLTDRMTAKIAANCIRSLLLNTNANNQPTSADHSISRHLLPRLIAFVTNTSPEDPEQARSLIAQTLVQFTAVVARGSSQDGGQGASIAMALVVPTLLSRAATEGAKDDAYRETSARLLELASADQTAFRAVVGAMSEGQRAFMEEVIKSGRSGGMGGGGGGAHVGAGGRRSEEDEEQAPTIALKMNFGG

Target ORF information:

RefSeq Version	XM_953928.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A HEAT repeat containing protein (NCU08993), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_953928.2

ORF Insert Sequence:

ATGTCATCCA CACCAGCTGA CGCCACCAAT GGCGAGCGCA ACGGCATCAC GAACACACTG 
ACATCCCACG CTACGACGAA TCCCGAGCTC GATCTTGCCA AGCTTCAAGC GCTCCCCGCC 
GAGCAGCAGG AGCTGTTCCT CCTGACGTCC GTCTCGGCAC TTAGCAGGCA TGTCCTGACG 
CTCGAACCAG ATGACTGCAC CGCGCAGCAG TTCTACATAA AAAAGGAGGC CTTCAAGGTC 
CTGAATCTTG CCTCGCCATC GCCAACAAGA GTCATACGAA ACGCGCTGGG GCGGTGTCTT 
GCGCACGTGT TCGACAAGGG CGATAGGAAG CTCCTGTTCG AAACTGTTAA CGAGCTGGTC 
GGCATCATCT CGAGTGGGAA ATTGAAGACG GAGGGTGAGA TACGGACAAA ACATGCTGCC 
GTGGCTTGCT TGGGGGATAT ATACGGCTCG GCTGGTGACA GCGCCATTGG CTTGCACCAG 
CTAGTATGCT CGGCGCTCTT GAAGCTATTG AAGGCAGCTT CGAACCACGC AGGGCTTCGT 
GCAGCTGTCC TGACCGCTTT CGGGAAGATT GTCACCATGA TACAGGGGAG CATGGACGAG 
AATGTTGCGA GAGATATATG GAAGCAGGGG AGAAACCATG CCTCTGGAGA CAAAGGATCA 
CTGGTCGTCG TGGCCGCTTG CCGATGCCTA CGGTCGCTTG TGCAGTATAC CCCCTATTTC 
AAGAACTCGA CCGACTTCGA CAAGCTCAAA TCGACCTTGT TCAAGACGTT CGACAATCCC 
TCATCAAACG CGCGATCGGC AGCAGCGGAC TGCTTTGCCC AAGCGCTGGT CGGGGGTTAC 
TCCGAGTCAG CCATTGGCGA GGCACCGCTG CCCCTGGTCA AGAAATCCAA GTCCAAGGCG 
ATCAAGAGAC AGTCAACAAT GGGAGGCCTT CAAGATGACG AGGAGGTTCC CTCACGGCCC 
GAGAGCCCTG CTCCAACCCA GAGACCCCAA GATTTGACCT TGTCGCTGGC CAATATACTC 
AAAACTTTGT CCACCCAATA CGTCCGCATT TCGACCACCA ATAAGGCAAG GGCTGCCATC 
GCTATTTGTA TCGGAAACGT TCTCCAGAAG CTCGGGGAAA AGACTGTAGA AGTCAACTAT 
CTTTGCATTC TCGAGATCCT TACAGCTGAC TTCTTGGGCC ATAACAACAT TCTCAACAAC 
CGGTACCGCT TACTCATTTC GCGGCGCATG GTCGATACCA TAATCCAGGA CATATTAGGA 
AAAAGGATCC TGGGTGAATC GGGGCAAATC ACGGCTGCTA AAGCTATCGT CAACGATATC 
CTCAAGAACT ATCCTCAGGC GCTCAAGGAA AAGCCGGAGC CATCCAAACA CACATTGGTT 
GTCTCACTGA GTGCTCTGTC CTCGCTGATC TCCAGTTTGG GCACAGCGGT GACCGACTTC 
TCAGAGGGAT GTCGCGATGG CCTGCTTCAA GTTCTCCAGC ACCCTAGCTA TACCGTTCAG 
GTATACGCCG CGGCCTGCAT GAAGAATTTT GTGTTGGCCT GCCCGCAACA GCTTCTTCCC 
TGTTTGTCAG TTTGCATGAA CAGCTTGAGC CGAGAACTGA GCCTTCTTGG GACGGGAAGA 
AATTCCCCTC GCAGGTGCAT TGGTTTCGCC CACGGCCTCG CTGCCGGGCT CAGCGCAAGC 
CCGCAACGCC CGCTCTATGG GTCGGTCGAG ATCAACAGTC GTGTTCTGAC CATGGCTACA 
AACTTGCTCA AGTCAAGTGG AACTTCGGAA CTACGGGTTG CAAGCACCCA GATCCAAGTT 
GCCTGGACCT TGATAGGCGG CCTAATGTCT CTTGGTCCCA ACTTCGTCAA GATTCATCTC 
TCGCAGCTGC TTTTACTCTG GAAGAATGCG CTGCCCAAGG TTCTGGCAAA GGATAGCATG 
GCGGTTCATA GGAATCTGCT CAATGCCTCG TTCCTCACAC ATGTCAGGGA ATGTGCGCTT 
GGGTCTATCC TAGCGTTCCT TCAGTTCAAC AGTCGCCTCT TGACTGTGGA CGTATGTAAG 
AGAATTGCTA CGATGTTGCA GAGTACTACT GCATTTCTCA AGACACTACC AGCGAAGAAG 
ACATCGGATG ATATAAGCGA GAGGCTGACT CCAGCCTTGC AACTCCAGGA TCTGGAGGTT 
ATGGTACGAC GCAGAGTCCT TCAGTGCTAT ACGAAGCTCG TGAACGTTAG CCCTGCTGGC 
GCCACTGAAG CACTCCTGCA GTCCAACCTT TTGACTCTCG CCATCTCTCT CTTTGCCGAC 
CCGGATAACT ATACACCGAG CTCCCTCAGC ACTTCCATCG CGAACGCCGC AGGTACCTTC 
GACTCTATTT GGGAGGTGGG CGACAATTCA GGGTTTGGAG TGACCAGTCT TGTTAGGGGC 
TTTGCGACAA GAAGCCTCCC GGGACAGCAG ACCAACGGCT CAGACAGCAC CCAACAAGAG 
TACGAACCGG AGGAGTGCAT CGAGAGGCTG CTTCTTTCTC CCGTATGCGG CACCCTGGAG 
AACGACGCTT GTCTTCTGTA CATCGGTAAC TCGGATAACC CGGGACTTCC TGATCCTGCT 
GCCACCGAGG TGGTTAACAT GGCTATCCAG TTGTTTGCCT TTGTCTTCCC TTTGACCCCA 
TCTAAGGTGC AGGAGAGTGC CTTGGAGCAA ATCACGACCT TCATAAGGGC TGGCTCCCTT 
CAGCGGGAAA CTGGCCGCAA GGCTGCTGTT GATGTCAACG TGGTTACGGC ACTTCTGTCT 
ACCCTCCGCG TGGCTTGCAA GGAGACCAAG TCGTCACCAG GCGACATCAC AAACATGGCC 
GTAGAGAAAC TTCTACAGGA GCTACTGAGA GATTTCGTAC TTGACCCCGA TCAGTACGTC 
CGTAGCATTG CTTACGAGGC AATTGCCAGA CTTTGCAGCG CTTGTGGCAA CGCATTCACG 
AATCAAGAGA TCAAGTTCCT TGTTGATACT ATTGTTGTCA ACCGAGAACC TAGCGCCAGA 
GCGGGCTGCG CCATGGCTCT TGGGTGCATC CAGACCAAGA TTGGCAGCAT GGCGGCTGGG 
TATCATTTGA AGACGATTCT TAACATCCTC ATGTCTCTGT GCAACGATCC GCACCCGACT 
GTTCATTACT GGGCTTTGGA GGCTATAGCA CGCGTATCAG ATGCTGCTGG ACTCGGCTTC 
TCCAGTTTCG TGTCTAGCAC ACTAGGAATG TTGGCCAAGC TCTACGTCTC GGATACGCAT 
AACCCTGAAT TAGCTTCTCC GATCACCATG AACTTGGAGG CTGACCTCTC CTCATCGGCT 
GCGATTGCTC GCTGCGTTGA TGCGCTCATC AACGTCCTTG GTCCAGATCT ACAAGACTCT 
ACGAAGTCTA GGGAGCTGAT CCTTACTCTT GTTGGGTATT TCCAGCAAGA AGAGGATCTT 
GAGATTCAGC GGGCAAGTCT CGCTTGCATG GAACATCTGA CATTGTATGC TCCGGGCTAC 
ATGGACTTCA TCGACTACGT CAGGACGCTT CAGCGATACA TCAAGTCCGA TCATGCCGCC 
CTCCGAGATG TGGCCGTGGA CAGTCTTTAT AATCTCATGA AGAGGAACTC GTACGATGTC 
ATCAAGGCCG CCGAGCAAGG CTTCGAGGAT CATCTGTGGC TGGTTCTCGA TGCCTCGCCG 
AGTCATGACG GAATGCGCAA CCTTATCAGA AACTGGCTCC GCCAGACCTG TCTTTCGGAC 
ACAGCCGCTT GGCTTGCGCG CTTCCAGCAT GTCCTGAAGA TGACCAGACC GAAAGCCTCC 
GCGCAGGCAA CCACGAACAC CGGGAGGTCT GGTGGAGGCA TTGACCTGCA GGACGAAGAG 
GTAGCTGGCT TCGCCGCTGC TGGAGGAGGC GCCAAGGACG ACAAAGATAC GCCGAGCGGC 
TCAGATGTGG AGCCCCTGAA GTGGCAGGTC ACTACCTTCG CCATGGACTG CCTGAACGAT 
ATCTTCATTT TGGTTACAAA AGATGTCGCA ACTCATGGCG AATCTGCTGC GCAGGCGGCC 
CTTCAGAACA AGGTTGCTGA TGTCGTTCGC ATGGCGTTCT CGGCGTCCAC GTCAGGTGTC 
TTGGACCAGA GAATCTGGGG CTTGAAGATC ATTGGCGCTG TTCTCAAAAT GTTTGGCAAG 
ATTCCAGACC CAGACTTTGA GGAGGCCATG CTGCTTGAGC AGTACCAAGC GCAAATCAGT 
TCCGCTCTTA CGCCCGCTTT TGCCGCGGAC TCGTCACCCG AACTGGCCTC GGAAGCCGTC 
GATGTTTGTG CAAGCTTCAT CGCCACAGGC ATTGTCACTG ACGTAGACAG GATGGGTAGA 
ATTCTGAAGA CGTTAGTGAG CTCGTTGGAG AACTTTGCAA AGGATGAGGA TAATGCTGGC 
ATTGGTGACC TCAAGGGACT CAGCTCAAAC GCACAGGTTA TGGTCAAGAT GTCTGTCTTC 
GCCGCCTGGG CCGAGCTACA AGTCGCCAGC TCTGAACAAA AGTACCTGCT GGATGTTCTC 
AAGCCTCATA TTGGTACCTT GACTCCTCTT TGGCTCGAGT CGTTACGTGA ATTTGCTCGG 
CTACGGTTCG AGCCGGATAT TTCCATGACG CTCGGCCCTC CATCGCTTTC TGGAAGCTTG 
GACACTGTCT ATGCAGCGCT GAACCGTGAG ACGTTGCTCA AGTTCTACCA GGAGTCTTGG 
TTGAAGTTAG TAGACGCTAT CGCCAGCCTC ATTGAACAGG ACAGCGAGTT CGTCTTTGAT 
GCCCTCGATG GCAAGGAGCT GTCAGGACCT ACCACCAACA GCAGCTCAAA GGGGGCAGAT 
ATCAACTACC GCGATGAGCC TGTTGCGTTC TTCTTTGTCT TGTTTGGTAT CGCGTTTGAA 
GCGCTCGCCA CGAGACCAGG CCAGAGCGAG TCTCTCGCTA CCCGAGAACA AACACTGGAG 
ATTCTCCGCG CTCTTAAGAA GATCCTTCAC CCAAGCGTAT CTGGTCATGC CATTTACCGC 
GATGCCATCT TCTCCGAGAC GATGGATCTG CTGGACCGTC TCGTACTCAC CGAAGGTCTT 
GATGTGCAGG GCGTTATCGT TGAGATTGCG CGTGCCCTAT GTGTGGCACA TCCAGCAGCC 
AGAAAGCAGG CTGACGACCC CGAGAACAGT GAGTTGTCTG ATGACATTGA GCAGCTCTTC 
GAGCTCACTC GGATCATCGT CTTGGTCCTC TCCGGACTGT TGCCCAATCT CAGTGAGACC 
CAACAGCCCG TGCGCCACCA GATGACAGAG GAAGCGATCC TTCTCGTCAA GACATCGCTC 
AACGCACTGG TCGATGCCGC GGAGGTGTTC CCATCCATAA TCAAGACGGA TCTACACGCC 
TGTATCCTGC ACATCTTTGC AACCATTCTT GCCACACCGT CATGCCAGGA GGTCATTGTC 
CCCCAAAACC TGTCGACACT CAAACGCTTT ATCAACGGCA TGACCGGTTC CAGGAGAGAC 
GGCGGAGATG ATGACGAGGG TAATTCGCCC ACGGACGTTC AGCTGCTGGG ATGTCTGCGC 
CGCTTCCTGT CCATCTATCT CAACGCTCAG AAGCGCGAAG CCCCGACGTC CTTGACCTGC 
GTCAAGAACT GTCTCCTGGC CATCACTATC CTCTTCACTG GTGGCAAAAA CCACCTGCCA 
GCCAGCGAGC CGCTGGTAGC CAAGTTCCTA GACGAGCTCG TAGACTGTCT AACTGACCGG 
ATGACCGCCA AAATAGCCGC AAACTGCATC CGCTCCCTCC TCCTCAACAC CAACGCCAAC 
AACCAACCCA CCTCAGCCGA TCACTCTATC TCCCGCCACC TCCTCCCGCG CCTCATTGCC 
TTCGTGACCA ACACCTCTCC TGAGGACCCG GAACAAGCTC GTTCGCTCAT CGCCCAAACC 
CTCGTGCAAT TTACCGCCGT GGTTGCCAGA GGATCGTCGC AGGACGGAGG CCAAGGCGCA 
TCCATTGCCA TGGCCCTCGT TGTGCCGACT TTGCTCTCGC GCGCGGCGAC AGAGGGAGCT 
AAAGATGATG CATACCGCGA GACGAGTGCA AGGCTACTGG AGTTGGCGAG CGCCGATCAG 
ACTGCTTTTA GGGCGGTGGT GGGCGCGATG AGTGAGGGCC AGAGGGCGTT TATGGAGGAG 
GTTATCAAGA GTGGACGATC GGGGGGAATG GGTGGTGGTG GTGGTGGCGC ACATGTAGGT 
GCCGGAGGGA GGAGAAGTGA GGAGGACGAA GAGCAGGCGC CGACTATTGC TTTGAAGATG 
AACTTTGGTG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU08993 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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