SPI1 cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPI1
Entrez Gene ID	104412408
Full Name	Spi-1 proto-oncogene
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010023129.1	XP_010021431.1	transcription factor PU.1 isoform X2
XM_010023128.1	XP_010021430.1	transcription factor PU.1 isoform X1

Pathways from BioSystems
Gene Ontology

The following SPI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe110936	XM_010023128.1 Latest version!	Nestor notabilis Spi-1 proto-oncogene (SPI1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
ONe110937	XM_010023129.1 Latest version!	Nestor notabilis Spi-1 proto-oncogene (SPI1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPI1 ( XM_010023128.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010021430.1 Nestor notabilis Spi-1 proto-oncogene (SPI1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONe110936
Clone ID Related Accession (Same CDS sequence)	XM_010023128.1
Accession Version	XM_010023128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	transcription factor PU.1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009913276.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCAAG CTTGCAAAAT GGAAGGATTT CCCCTCATTC CCCCTCAACC CTCTGAAGAT 
ATGGTACCCT ATGAGTCAGA CCTCTACAGA CAGCCCCATG ACTATTACCA GTATCTCAAC 
AGTGATGGAG AGAGTCATGG TGATCATTAC TGGGAATACC ATCCACACCA CATGCACAGT 
GAGTTTGAGA CCTTTGGAGA CAACCACTTC ACAGAACTGC AGAGTGTTCA GCCTCCCCAG 
CTGCAGCAGC TGTACAGGCA CATGGAGATC GAACAAATGC ATGTCTTGGA TTCTACAATC 
CCAACCACAC ACATCGGACT CAACCACCAG GTGTCCTATT TGCCTCGGAT GTGCCTACAG 
TACTCCTCTC CACCGCAGCC CAGTTCTGAT GAAGAGGACA TAGAGAGGCA GAGCCCCCCA 
CTGGAAGTAT CAGATGGAGA GACTGATGGT GTGGATCCTG GGCCTGGAAT TATGCATGGA 
GAAACAGGCA GCAAGAAGAA GATACGTCTC TATCAGTTCC TTCTGGACCT TCTTCGCAGT 
GGAGACATGA AGGACAGCAT CTGGTGGGTG GACAAGGAAA AAGGTACTTT CCAGTTCTCC 
TCCAAACACA AAGAAGCATT GGCACATCGC TGGGGCATCC AGAAAGGCAA CCGCAAGAAA 
ATGACCTACC AGAAGATGGC ACGGGCTTTG AGAAACTACG GCAAGACAGG GGAGGTGAAG 
AAGGTCAAGA AGAAGCTGAC CTATCAGTTT AGTGGAGAGG TGATGGGAAG GGGGGTCACT 
GATAGGAAGC ATTATCCTCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010021430.1
CDS	1..804
Translation	MLQACKMEGFPLIPPQPSEDMVPYESDLYRQPHDYYQYLNSDGESHGDHYWEYHPHHMHSEFETFGDNHFTELQSVQPPQLQQLYRHMEIEQMHVLDSTIPTTHIGLNHQVSYLPRMCLQYSSPPQPSSDEEDIERQSPPLEVSDGETDGVDPGPGIMHGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKEKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVMGRGVTDRKHYPH

Target ORF information:

RefSeq Version	XM_010023128.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis Spi-1 proto-oncogene (SPI1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010023128.1

ORF Insert Sequence:

ATGTTGCAAG CTTGCAAAAT GGAAGGATTT CCCCTCATTC CCCCTCAACC CTCTGAAGAT 
ATGGTACCCT ATGAGTCAGA CCTCTACAGA CAGCCCCATG ACTATTACCA GTATCTCAAC 
AGTGATGGAG AGAGTCATGG TGATCATTAC TGGGAATACC ATCCACACCA CATGCACAGT 
GAGTTTGAGA CCTTTGGAGA CAACCACTTC ACAGAACTGC AGAGTGTTCA GCCTCCCCAG 
CTGCAGCAGC TGTACAGGCA CATGGAGATC GAACAAATGC ATGTCTTGGA TTCTACAATC 
CCAACCACAC ACATCGGACT CAACCACCAG GTGTCCTATT TGCCTCGGAT GTGCCTACAG 
TACTCCTCTC CACCGCAGCC CAGTTCTGAT GAAGAGGACA TAGAGAGGCA GAGCCCCCCA 
CTGGAAGTAT CAGATGGAGA GACTGATGGT GTGGATCCTG GGCCTGGAAT TATGCATGGA 
GAAACAGGCA GCAAGAAGAA GATACGTCTC TATCAGTTCC TTCTGGACCT TCTTCGCAGT 
GGAGACATGA AGGACAGCAT CTGGTGGGTG GACAAGGAAA AAGGTACTTT CCAGTTCTCC 
TCCAAACACA AAGAAGCATT GGCACATCGC TGGGGCATCC AGAAAGGCAA CCGCAAGAAA 
ATGACCTACC AGAAGATGGC ACGGGCTTTG AGAAACTACG GCAAGACAGG GGAGGTGAAG 
AAGGTCAAGA AGAAGCTGAC CTATCAGTTT AGTGGAGAGG TGATGGGAAG GGGGGTCACT 
GATAGGAAGC ATTATCCTCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPI1 ( XM_010023129.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010021431.1 Nestor notabilis Spi-1 proto-oncogene (SPI1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONe110937
Clone ID Related Accession (Same CDS sequence)	XM_010023129.1
Accession Version	XM_010023129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	transcription factor PU.1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009913276.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCAAG CTTGCAAAAT GGAAGGATTT CCCCTCATTC CCCCTCCCTC TGAAGATATG 
GTACCCTATG AGTCAGACCT CTACAGACAG CCCCATGACT ATTACCAGTA TCTCAACAGT 
GATGGAGAGA GTCATGGTGA TCATTACTGG GAATACCATC CACACCACAT GCACAGTGAG 
TTTGAGACCT TTGGAGACAA CCACTTCACA GAACTGCAGA GTGTTCAGCC TCCCCAGCTG 
CAGCAGCTGT ACAGGCACAT GGAGATCGAA CAAATGCATG TCTTGGATTC TACAATCCCA 
ACCACACACA TCGGACTCAA CCACCAGGTG TCCTATTTGC CTCGGATGTG CCTACAGTAC 
TCCTCTCCAC CGCAGCCCAG TTCTGATGAA GAGGACATAG AGAGGCAGAG CCCCCCACTG 
GAAGTATCAG ATGGAGAGAC TGATGGTGTG GATCCTGGGC CTGGAATTAT GCATGGAGAA 
ACAGGCAGCA AGAAGAAGAT ACGTCTCTAT CAGTTCCTTC TGGACCTTCT TCGCAGTGGA 
GACATGAAGG ACAGCATCTG GTGGGTGGAC AAGGAAAAAG GTACTTTCCA GTTCTCCTCC 
AAACACAAAG AAGCATTGGC ACATCGCTGG GGCATCCAGA AAGGCAACCG CAAGAAAATG 
ACCTACCAGA AGATGGCACG GGCTTTGAGA AACTACGGCA AGACAGGGGA GGTGAAGAAG 
GTCAAGAAGA AGCTGACCTA TCAGTTTAGT GGAGAGGTGA TGGGAAGGGG GGTCACTGAT 
AGGAAGCATT ATCCTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010021431.1
CDS	291..1091
Translation	MLQACKMEGFPLIPPPSEDMVPYESDLYRQPHDYYQYLNSDGESHGDHYWEYHPHHMHSEFETFGDNHFTELQSVQPPQLQQLYRHMEIEQMHVLDSTIPTTHIGLNHQVSYLPRMCLQYSSPPQPSSDEEDIERQSPPLEVSDGETDGVDPGPGIMHGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKEKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVMGRGVTDRKHYPH

Target ORF information:

RefSeq Version	XM_010023129.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis Spi-1 proto-oncogene (SPI1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010023129.1

ORF Insert Sequence:

ATGTTGCAAG CTTGCAAAAT GGAAGGATTT CCCCTCATTC CCCCTCCCTC TGAAGATATG 
GTACCCTATG AGTCAGACCT CTACAGACAG CCCCATGACT ATTACCAGTA TCTCAACAGT 
GATGGAGAGA GTCATGGTGA TCATTACTGG GAATACCATC CACACCACAT GCACAGTGAG 
TTTGAGACCT TTGGAGACAA CCACTTCACA GAACTGCAGA GTGTTCAGCC TCCCCAGCTG 
CAGCAGCTGT ACAGGCACAT GGAGATCGAA CAAATGCATG TCTTGGATTC TACAATCCCA 
ACCACACACA TCGGACTCAA CCACCAGGTG TCCTATTTGC CTCGGATGTG CCTACAGTAC 
TCCTCTCCAC CGCAGCCCAG TTCTGATGAA GAGGACATAG AGAGGCAGAG CCCCCCACTG 
GAAGTATCAG ATGGAGAGAC TGATGGTGTG GATCCTGGGC CTGGAATTAT GCATGGAGAA 
ACAGGCAGCA AGAAGAAGAT ACGTCTCTAT CAGTTCCTTC TGGACCTTCT TCGCAGTGGA 
GACATGAAGG ACAGCATCTG GTGGGTGGAC AAGGAAAAAG GTACTTTCCA GTTCTCCTCC 
AAACACAAAG AAGCATTGGC ACATCGCTGG GGCATCCAGA AAGGCAACCG CAAGAAAATG 
ACCTACCAGA AGATGGCACG GGCTTTGAGA AACTACGGCA AGACAGGGGA GGTGAAGAAG 
GTCAAGAAGA AGCTGACCTA TCAGTTTAGT GGAGAGGTGA TGGGAAGGGG GGTCACTGAT 
AGGAAGCATT ATCCTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.