ZW10 cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZW10
Entrez Gene ID	104399743
Full Name	zw10 kinetochore protein
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010014093.1	XP_010012395.1	centromere/kinetochore protein zw10 homolog

Pathways from BioSystems
Gene Ontology

The following ZW10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZW10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe114075	XM_010014093.1 Latest version!	Nestor notabilis zw10 kinetochore protein (ZW10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZW10 ( XM_010014093.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010012395.1 Nestor notabilis zw10 kinetochore protein (ZW10), mRNA.

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CloneID	ONe114075
Clone ID Related Accession (Same CDS sequence)	XM_010014093.1
Accession Version	XM_010014093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	centromere/kinetochore protein zw10 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009927525.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAACA AGAAGTACAA CGAGTTCCTG CCGAGCATGC AGAGCGCCGA GGAGCTGGTG 
TCGCAGGTGG AGGGGCTGTC CAGCAACATC GACCTGCTCA AAGCGGGCAT CGAGAACGAG 
GTTCAGCGAG ATCTCAACGT GGCTGTTGCC GAGTTTACGG AACTGAAGCA GCAGCTGGAG 
CGAGACACGC TGGTTCTGAG TGTCCTGAAG AAGCTGCAGG AGTTTGATAT CGCTATGAAA 
GGGTACAACA CTGCCTTACT GGAAAAGAAG TACGTTGCAG CAGCTCAGCA GCTGGAAACG 
GCACGAAGCA GCCTCAAACT GCTGGAATCC CGCAAGGGCT TTGAGCTGAA GATCCTGAAG 
GCACTAGGCA CCGAGCTGAC AGTGCAGACA CAAAACATGC TCTATCATCT GGGAGAGGAG 
TGGCAGAAAT TGGCTGTATG GAAGCTTCCT CCTTCCAAAG AAAGCAGCAG CCTGGAGTCA 
GTGGTGCATT CAGAATTGCA CCTACACACA GTGCCATCAA AAGAGGATGA GATTGCCGGC 
CCCCCTGTTG CTTCTGTGCT GCAGGCATTT GCTGTCCTGG GAGAACTGCA CACCAAGCTT 
AAAATCTTTG GCCAGTTGTT GCTGAAATAC ATCCTCAAGC CACTGATTAC ATACCCATCT 
CTTCAGCCAT GCACAGAGGA GCAGTCCGAT GTGTTCATCC TACGTTTTAA GTCTGAGGAA 
CCTAGCTTGG ATCATTCCTC TCCTATGGAG GTTTTTGACA AGATCAAGCT TATTTTTGAA 
GTTCTCCACA AGTATCTGCT AAATGTGCCT CTGGAACAGC CTCTGGAAGA TAAGAAGGGT 
TGTGGAGTTA CACTGGCAGA ACTGCTAGGT GATATGATAT GGGAAGAGTT ATCAGACTGC 
CTCATTCAGA ACTGTCTGGT GAATTCAATC CCAACCAATA GCAGCAAACT AGAGCAGTAT 
ATAGAGGTGA TTAAATCCAC AGAGGAATTT GAGAAAGCCT TGAAGAACAT GCAGTTTTTG 
AAAGGAGACA CAACTGATTT ACTGAAGTAC GCCCGTAATG TCAACTCCCA CTTTGCTAAC 
AAGAAATGTC AGGATGTGAT TGTGGCAGCC AGAAACCTGA TGACCTCAGA AATACACAAT 
ACTGTAAAGA TCACACCTGA TTCCTGTGTG GCCCTACCAA GGCTTCCTGA TCCTGGTACA 
GGAGATCACT CCAAAATGCC AAAAGCTTCT GAACTTCTGC ACAACGACAT GGTGAATTTG 
GAGAATAAGA CTAAACTGAG CCAGTACACA TTTTCTCTGC CCACATGCCG CATCAGTTCA 
TCAGTGGAGA AGCTGATGGA GTTGGCCTAC CAGACATTGT TGGAGGCTAC ATCCAGCACA 
GATCAGTGCT GCATACAGCT CTTCTACTCT GTACGGAATA TATTCCAGCT GTTCTATGAT 
GTAGTGCCGA CGTACCACAA GGAGAACCTT CAGAAATTAC CCCAACTGGC AGCTATTCAC 
CACAACAACT GCATGTATAT TGCTCACCAC TTGCTCACCC TGGGACACCA GTTCCGATAC 
CGCCTGACTA ACATCCTGTG CGACGGAGCA GCTACGTTTG TAGACCTGGT GCCTGGTTTT 
AGGAGACTTG GGATGGAGTG TTTTCTGGCC CAGATGCGAG TGCAGAAAGG AGAAATCCTG 
GAGAGGCTGT CAAGTGCCAG GAATTTTTCT AATATGGATG ATGAGGAAAA TTACTGTGCA 
GCTAACAAAG CAATAAGGCA GGTATTACAT CAGTTGAAAA GACTGGGAAA AGTCTGGCAA 
GATGTTCTTC CAGTGAATGT GTACTGCAAG GCCATGGGGA CTTTACTGAA CACAGCTCTC 
ACGGAGATTG TCACTAGGAT CACTGCCCTA GAGGATATCT CTGCCGAAGA TGCAGATAGG 
TTATACTCCC TCTGTAGGAC CATGGTGGAG GAAGGACCCC AAGTTTTCAC CCCGTTACCT 
GAAGATGACA GAAATAAGAA GTACCAAGAG GAAGTTCCAG TCTACGTGCA GAAGTGGATG 
ACATTCAAAG AGCTGATGAT CATCTTGCAA GCCAACCTCC AAGAAATCGT AGATCAGTGG 
GCGGATGGGA AGGGACCTCT TGCAGCTGAA TTCTCAGCAG CTGAAGTGAA GAGTCTGATC 
CGAGCCTTGT TCCAGAACAC AGAAAGGAGA GCAGCAGCAC TTGCCAAAAT TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010012395.1
CDS	23..2239
Translation	MINKKYNEFLPSMQSAEELVSQVEGLSSNIDLLKAGIENEVQRDLNVAVAEFTELKQQLERDTLVLSVLKKLQEFDIAMKGYNTALLEKKYVAAAQQLETARSSLKLLESRKGFELKILKALGTELTVQTQNMLYHLGEEWQKLAVWKLPPSKESSSLESVVHSELHLHTVPSKEDEIAGPPVASVLQAFAVLGELHTKLKIFGQLLLKYILKPLITYPSLQPCTEEQSDVFILRFKSEEPSLDHSSPMEVFDKIKLIFEVLHKYLLNVPLEQPLEDKKGCGVTLAELLGDMIWEELSDCLIQNCLVNSIPTNSSKLEQYIEVIKSTEEFEKALKNMQFLKGDTTDLLKYARNVNSHFANKKCQDVIVAARNLMTSEIHNTVKITPDSCVALPRLPDPGTGDHSKMPKASELLHNDMVNLENKTKLSQYTFSLPTCRISSSVEKLMELAYQTLLEATSSTDQCCIQLFYSVRNIFQLFYDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRYRLTNILCDGAATFVDLVPGFRRLGMECFLAQMRVQKGEILERLSSARNFSNMDDEENYCAANKAIRQVLHQLKRLGKVWQDVLPVNVYCKAMGTLLNTALTEIVTRITALEDISAEDADRLYSLCRTMVEEGPQVFTPLPEDDRNKKYQEEVPVYVQKWMTFKELMIILQANLQEIVDQWADGKGPLAAEFSAAEVKSLIRALFQNTERRAAALAKIK

Target ORF information:

RefSeq Version	XM_010014093.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis zw10 kinetochore protein (ZW10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010014093.1

ORF Insert Sequence:

ATGATCAACA AGAAGTACAA CGAGTTCCTG CCGAGCATGC AGAGCGCCGA GGAGCTGGTG 
TCGCAGGTGG AGGGGCTGTC CAGCAACATC GACCTGCTCA AAGCGGGCAT CGAGAACGAG 
GTTCAGCGAG ATCTCAACGT GGCTGTTGCC GAGTTTACGG AACTGAAGCA GCAGCTGGAG 
CGAGACACGC TGGTTCTGAG TGTCCTGAAG AAGCTGCAGG AGTTTGATAT CGCTATGAAA 
GGGTACAACA CTGCCTTACT GGAAAAGAAG TACGTTGCAG CAGCTCAGCA GCTGGAAACG 
GCACGAAGCA GCCTCAAACT GCTGGAATCC CGCAAGGGCT TTGAGCTGAA GATCCTGAAG 
GCACTAGGCA CCGAGCTGAC AGTGCAGACA CAAAACATGC TCTATCATCT GGGAGAGGAG 
TGGCAGAAAT TGGCTGTATG GAAGCTTCCT CCTTCCAAAG AAAGCAGCAG CCTGGAGTCA 
GTGGTGCATT CAGAATTGCA CCTACACACA GTGCCATCAA AAGAGGATGA GATTGCCGGC 
CCCCCTGTTG CTTCTGTGCT GCAGGCATTT GCTGTCCTGG GAGAACTGCA CACCAAGCTT 
AAAATCTTTG GCCAGTTGTT GCTGAAATAC ATCCTCAAGC CACTGATTAC ATACCCATCT 
CTTCAGCCAT GCACAGAGGA GCAGTCCGAT GTGTTCATCC TACGTTTTAA GTCTGAGGAA 
CCTAGCTTGG ATCATTCCTC TCCTATGGAG GTTTTTGACA AGATCAAGCT TATTTTTGAA 
GTTCTCCACA AGTATCTGCT AAATGTGCCT CTGGAACAGC CTCTGGAAGA TAAGAAGGGT 
TGTGGAGTTA CACTGGCAGA ACTGCTAGGT GATATGATAT GGGAAGAGTT ATCAGACTGC 
CTCATTCAGA ACTGTCTGGT GAATTCAATC CCAACCAATA GCAGCAAACT AGAGCAGTAT 
ATAGAGGTGA TTAAATCCAC AGAGGAATTT GAGAAAGCCT TGAAGAACAT GCAGTTTTTG 
AAAGGAGACA CAACTGATTT ACTGAAGTAC GCCCGTAATG TCAACTCCCA CTTTGCTAAC 
AAGAAATGTC AGGATGTGAT TGTGGCAGCC AGAAACCTGA TGACCTCAGA AATACACAAT 
ACTGTAAAGA TCACACCTGA TTCCTGTGTG GCCCTACCAA GGCTTCCTGA TCCTGGTACA 
GGAGATCACT CCAAAATGCC AAAAGCTTCT GAACTTCTGC ACAACGACAT GGTGAATTTG 
GAGAATAAGA CTAAACTGAG CCAGTACACA TTTTCTCTGC CCACATGCCG CATCAGTTCA 
TCAGTGGAGA AGCTGATGGA GTTGGCCTAC CAGACATTGT TGGAGGCTAC ATCCAGCACA 
GATCAGTGCT GCATACAGCT CTTCTACTCT GTACGGAATA TATTCCAGCT GTTCTATGAT 
GTAGTGCCGA CGTACCACAA GGAGAACCTT CAGAAATTAC CCCAACTGGC AGCTATTCAC 
CACAACAACT GCATGTATAT TGCTCACCAC TTGCTCACCC TGGGACACCA GTTCCGATAC 
CGCCTGACTA ACATCCTGTG CGACGGAGCA GCTACGTTTG TAGACCTGGT GCCTGGTTTT 
AGGAGACTTG GGATGGAGTG TTTTCTGGCC CAGATGCGAG TGCAGAAAGG AGAAATCCTG 
GAGAGGCTGT CAAGTGCCAG GAATTTTTCT AATATGGATG ATGAGGAAAA TTACTGTGCA 
GCTAACAAAG CAATAAGGCA GGTATTACAT CAGTTGAAAA GACTGGGAAA AGTCTGGCAA 
GATGTTCTTC CAGTGAATGT GTACTGCAAG GCCATGGGGA CTTTACTGAA CACAGCTCTC 
ACGGAGATTG TCACTAGGAT CACTGCCCTA GAGGATATCT CTGCCGAAGA TGCAGATAGG 
TTATACTCCC TCTGTAGGAC CATGGTGGAG GAAGGACCCC AAGTTTTCAC CCCGTTACCT 
GAAGATGACA GAAATAAGAA GTACCAAGAG GAAGTTCCAG TCTACGTGCA GAAGTGGATG 
ACATTCAAAG AGCTGATGAT CATCTTGCAA GCCAACCTCC AAGAAATCGT AGATCAGTGG 
GCGGATGGGA AGGGACCTCT TGCAGCTGAA TTCTCAGCAG CTGAAGTGAA GAGTCTGATC 
CGAGCCTTGT TCCAGAACAC AGAAAGGAGA GCAGCAGCAC TTGCCAAAAT TAAGTAA