Ankrd52 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ankrd52
Entrez Gene ID	103746512
Full Name	ankyrin repeat domain 52
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017802080.1	XP_017657569.1	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C isoform X2
XM_008848716.2	XP_008846938.1	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C

Pathways from BioSystems
Gene Ontology

The following Ankrd52 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankrd52 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa31325	XM_008848716.2 Latest version!	Nannospalax galili ankyrin repeat domain 52 (Ankrd52), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ONa31326	XM_017802080.1 Latest version!	Nannospalax galili ankyrin repeat domain 52 (Ankrd52), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ankrd52 ( XM_008848716.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008846938.1 Nannospalax galili ankyrin repeat domain 52 (Ankrd52), mRNA.

Price & Availability↑

CloneID	ONa31325
Clone ID Related Accession (Same CDS sequence)	XM_008848716.2
Accession Version	XM_008848716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351698.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008848716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATCC TCAGCATCAC AGACCAGCCG CCCCTGGTCC AGGCCATCTT TAGCCGAGAT 
GTGGAGGAAG TGCGTTCCCT CCTCTCGCAG AAGGAGAACA TCAATGTGCT GGACCAAGAG 
AGGCGAACCC CATTGCATGC TGCTGCCTAC GTAGGCGATG TCCCCATCCT CCAGTTGCTA 
CTGATGTCAG GTGCTAATGT CAACGCTAAG GACACACTGT GGCTGACCCC TCTTCATCGT 
GCTGCTGCCT CCCGAAACGA GAAGGTGCTG GGGCTGCTGC TGGCACATTC AGCTGATGTG 
AATGCCCGGG ACAAGCTGTG GCAGACACCA CTGCATGTGG CTGCTGCCAA CCGGGCCACC 
AAGTGTGCTG AGGCTCTGGC ACCCCTGTTG AGCAGCCTCA ATGTGGCTGA CAGGAGCGGG 
CGCAGTGCTC TACACCATGC AGTGCACAGT GGGCACCTTG AGACAGTGAA CCTGCTTCTC 
AACAAGGGAG CCAGCCTGAA TGTCTGCGAT AAAAAGGAGC GGCAGCCTCT GCACTGGGCA 
GCTTTTCTGG GGCATTTGGA GGTCCTGAAA CTGCTGGTGG CTCGTGGAGC AGACCTCGGC 
TGTAAGGACC GCAAGGGCTA TGGGCTGCTC CACACAGCCG CAGCCAGTGG CCAGATTGAA 
GTGGTGAAAT ACTTGCTCCG GATGGGGGCC GAGATTGATG AGCCCAATGC TTTTGGAAAC 
ACAGCTTTGC ACATTGCCTG CTACCTGGGC CAGGATGCTG TGGCTATTGA ACTGGTGAAT 
GCAGGAGCCA ATGTCAACCA GCCAAATGAC AAAGGCTTCA CACCACTGCA CGTGGCTGCG 
GTCTCCACAA ATGGCGCTCT CTGCTTGGAG CTGCTGGTCA ATAATGGGGC TGATGTCAAC 
TACCAGAGCA AAGAAGGGAA AAGTCCTCTG CACATGGCCG CCATCCACGG CCGTTTCACA 
CGCTCCCAGA TCCTCATTCA GAATGGTAGC GAGATTGATT GTGCTGACAA ATTTGGGAAC 
ACGCCACTAC ATGTGGCTGC TCGCTACGGA CACGAGCTGC TCATCAGCAC TCTCATGACC 
AATGGCGCGG ATACCGCCCG GCGCGGCATC CACGACATGT TCCCCCTGCA CTTAGCTGTT 
CTCTTTGGAT TCTCTGACTG TTGCCGGAAG CTTCTTTCCT CAGGTCAGCT GTACAGCATT 
GTGTCTTCAC TCAGCAATGA GCATGTGCTT TCCGCTGGGT TTGACATCAA CACACCTGAC 
AACCTTGGCC GCACCTGTCT TCATGCTGCT GCTTCTGGAG GGAATGTTGA ATGTCTTAAT 
TTGCTGTTGA GCAGTGGGGC TGACCTAAGA AGGAGAGACA AATTTGGAAG GACTCCACTG 
CACTATGCAG CGGCCAATGG TAGCTACCAG TGTGCAGTGA CATTGGTGAC TGCTGGGGCA 
GGCGTCAATG AGGCTGACTG TAAAGGCTGC TCTCCCCTCC ACTACGCTGC TGCCTCTGAT 
ACCTACCGGA GAGCGGAACC CCACACTGCT TCCAGCCATG ATGCTGAAGA GGATGAGCTA 
CTGAAGGAGT CCCGTAGGAA GGAGGCCTTC TTCTGTCTGG AGTTCTTATT GGATAATGGT 
GCAGACCCCT CCCTGCGGGA CAGGCAGGGC TACACAGCTG TGCACTATGC AGCCGCCTAC 
GGCAACAGAC AGAACCTCGA ACTGCTCTTA GAAATGTCCT TTAACTGCCT GGAGGATGTG 
GAGAGCACCA TTCCAGTCAG CCCTTTGCAC TTAGCTGCCT ACAATGGTCA CTGTGAAGCT 
CTGAAGACAC TGGCCGAGAC GCTGGTGAAT CTGGATGTAA GGGACCACAA GGGCCGAACT 
GCGCTCTTCC TAGCCACTGA GCGAGGCTCT ACTGAGTGTG TGGAGGTGCT TACAGCCCAT 
GGCGCTTCTG CCCTCATCAA GGAGCGGAAG CGCAAGTGGA CACCCCTACA TGCTGCTGCT 
GCCTCTGGCC ACACTGATTC CCTGCACTTG CTGATTGACA GTGGGGAACG AGCTGACATC 
ACAGATGTCA TGGATGCCTA TGGACAAACC CCACTGATGC TAGCCATCAT GAATGGCCAT 
GTGGACTGTG TACATCTGCT GCTAGAGAAA GGATCCACAG CTGATGCTGC TGACCTCCGG 
GGCCGAACTG CCCTTCACCG TGGGGCAGTA ACTGGCTGTG AGGACTGCCT GGCCGCCCTG 
CTGGACCATG ATGCATTTGT ACTGTGCCGA GACTTTAAGG GCCGAACACC CATTCACCTG 
GCCTCAGCAT GTGGCCATAC TGCAGTGCTG CGGACTCTAC TGCAGGCTGC CCTTTCCACA 
GACCCCCTGG ATGCTGGCGT AGATTACAGC GGATACTCGC CCATGCACTG GGCCTCCTAC 
ACTGGACATG AAGATTGTCT GGAGTTGTTA CTTGAACACA GCCCATTCTC ATACTTGGAG 
GGAAACCCCT TCACTCCTTT GCACTGTGCA GTAATTAATA ACCAGGACAG CACCACAGAG 
ATGTTGCTGG GAGCTCTGGG TGCCAAGATT GTGAACAGCC GAGATGCCAA AGGACGGACC 
CCCCTTCACG CCGCTGCCTT TGCGGACAAT GTGTCTGGGC TCCGGATGCT GCTGCAGCAT 
CAAGCTGAGG TGAATGCCAC TGACCACACT GGCCGCACTG CACTCATGAC GGCAGCTGAG 
AATGGGCAGA CCGCTGCTGT GGAATTTCTG TTGTATCGAG GGAAGGCAGA CCTGACTGTG 
CTGGATGAGA ACAAGAACAC TGCCCTCCAC TTGGCTTGTA GCAAGGGCCA TGAGAAATGT 
GCCCTCATGA TCCTGGCAGA AACTCAAGAC CTTGGCCTTA TCAATGCTAC CAACAGTGCG 
CTGCAGATGC CACTCCACAT TGCTGCCCGG AATGGTTTAG CTTCTGTGGT GCAGGCCCTG 
CTAAGTCGTG GGGCCACAGT GCTGGCTGTG GATGAAGAAG GTCACACCCC AGCACTGGCC 
TGTGCTCCCA ACAAAGATGT GGCAGACTGC CTGGCCTTGA TCCTTTCCAC CATGAAGCCT 
TTCCCACCCA AGGACGCCGT CAGTCCTTTC AGCTTCAGCC TGCTCAAGAA CTGCGGCATC 
GCAGCTGCCA AGACGGTGGG TGGCTGTGGC GCCCTGCCCC ACGGGGCGTC CTGCCCCTAT 
AGCCAGGAAC GGCATGGCGC CATTGGGTTA GATGGCTGCT ACTCTGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008846938.1
CDS	82..3312
Translation	MGILSITDQPPLVQAIFSRDVEEVRSLLSQKENINVLDQERRTPLHAAAYVGDVPILQLLLMSGANVNAKDTLWLTPLHRAAASRNEKVLGLLLAHSADVNARDKLWQTPLHVAAANRATKCAEALAPLLSSLNVADRSGRSALHHAVHSGHLETVNLLLNKGASLNVCDKKERQPLHWAAFLGHLEVLKLLVARGADLGCKDRKGYGLLHTAAASGQIEVVKYLLRMGAEIDEPNAFGNTALHIACYLGQDAVAIELVNAGANVNQPNDKGFTPLHVAAVSTNGALCLELLVNNGADVNYQSKEGKSPLHMAAIHGRFTRSQILIQNGSEIDCADKFGNTPLHVAARYGHELLISTLMTNGADTARRGIHDMFPLHLAVLFGFSDCCRKLLSSGQLYSIVSSLSNEHVLSAGFDINTPDNLGRTCLHAAASGGNVECLNLLLSSGADLRRRDKFGRTPLHYAAANGSYQCAVTLVTAGAGVNEADCKGCSPLHYAAASDTYRRAEPHTASSHDAEEDELLKESRRKEAFFCLEFLLDNGADPSLRDRQGYTAVHYAAAYGNRQNLELLLEMSFNCLEDVESTIPVSPLHLAAYNGHCEALKTLAETLVNLDVRDHKGRTALFLATERGSTECVEVLTAHGASALIKERKRKWTPLHAAAASGHTDSLHLLIDSGERADITDVMDAYGQTPLMLAIMNGHVDCVHLLLEKGSTADAADLRGRTALHRGAVTGCEDCLAALLDHDAFVLCRDFKGRTPIHLASACGHTAVLRTLLQAALSTDPLDAGVDYSGYSPMHWASYTGHEDCLELLLEHSPFSYLEGNPFTPLHCAVINNQDSTTEMLLGALGAKIVNSRDAKGRTPLHAAAFADNVSGLRMLLQHQAEVNATDHTGRTALMTAAENGQTAAVEFLLYRGKADLTVLDENKNTALHLACSKGHEKCALMILAETQDLGLINATNSALQMPLHIAARNGLASVVQALLSRGATVLAVDEEGHTPALACAPNKDVADCLALILSTMKPFPPKDAVSPFSFSLLKNCGIAAAKTVGGCGALPHGASCPYSQERHGAIGLDGCYSE

Target ORF information:

RefSeq Version	XM_008848716.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili ankyrin repeat domain 52 (Ankrd52), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008848716.2

ORF Insert Sequence:

ATGGGGATCC TCAGCATCAC AGACCAGCCG CCCCTGGTCC AGGCCATCTT TAGCCGAGAT 
GTGGAGGAAG TGCGTTCCCT CCTCTCGCAG AAGGAGAACA TCAATGTGCT GGACCAAGAG 
AGGCGAACCC CATTGCATGC TGCTGCCTAC GTAGGCGATG TCCCCATCCT CCAGTTGCTA 
CTGATGTCAG GTGCTAATGT CAACGCTAAG GACACACTGT GGCTGACCCC TCTTCATCGT 
GCTGCTGCCT CCCGAAACGA GAAGGTGCTG GGGCTGCTGC TGGCACATTC AGCTGATGTG 
AATGCCCGGG ACAAGCTGTG GCAGACACCA CTGCATGTGG CTGCTGCCAA CCGGGCCACC 
AAGTGTGCTG AGGCTCTGGC ACCCCTGTTG AGCAGCCTCA ATGTGGCTGA CAGGAGCGGG 
CGCAGTGCTC TACACCATGC AGTGCACAGT GGGCACCTTG AGACAGTGAA CCTGCTTCTC 
AACAAGGGAG CCAGCCTGAA TGTCTGCGAT AAAAAGGAGC GGCAGCCTCT GCACTGGGCA 
GCTTTTCTGG GGCATTTGGA GGTCCTGAAA CTGCTGGTGG CTCGTGGAGC AGACCTCGGC 
TGTAAGGACC GCAAGGGCTA TGGGCTGCTC CACACAGCCG CAGCCAGTGG CCAGATTGAA 
GTGGTGAAAT ACTTGCTCCG GATGGGGGCC GAGATTGATG AGCCCAATGC TTTTGGAAAC 
ACAGCTTTGC ACATTGCCTG CTACCTGGGC CAGGATGCTG TGGCTATTGA ACTGGTGAAT 
GCAGGAGCCA ATGTCAACCA GCCAAATGAC AAAGGCTTCA CACCACTGCA CGTGGCTGCG 
GTCTCCACAA ATGGCGCTCT CTGCTTGGAG CTGCTGGTCA ATAATGGGGC TGATGTCAAC 
TACCAGAGCA AAGAAGGGAA AAGTCCTCTG CACATGGCCG CCATCCACGG CCGTTTCACA 
CGCTCCCAGA TCCTCATTCA GAATGGTAGC GAGATTGATT GTGCTGACAA ATTTGGGAAC 
ACGCCACTAC ATGTGGCTGC TCGCTACGGA CACGAGCTGC TCATCAGCAC TCTCATGACC 
AATGGCGCGG ATACCGCCCG GCGCGGCATC CACGACATGT TCCCCCTGCA CTTAGCTGTT 
CTCTTTGGAT TCTCTGACTG TTGCCGGAAG CTTCTTTCCT CAGGTCAGCT GTACAGCATT 
GTGTCTTCAC TCAGCAATGA GCATGTGCTT TCCGCTGGGT TTGACATCAA CACACCTGAC 
AACCTTGGCC GCACCTGTCT TCATGCTGCT GCTTCTGGAG GGAATGTTGA ATGTCTTAAT 
TTGCTGTTGA GCAGTGGGGC TGACCTAAGA AGGAGAGACA AATTTGGAAG GACTCCACTG 
CACTATGCAG CGGCCAATGG TAGCTACCAG TGTGCAGTGA CATTGGTGAC TGCTGGGGCA 
GGCGTCAATG AGGCTGACTG TAAAGGCTGC TCTCCCCTCC ACTACGCTGC TGCCTCTGAT 
ACCTACCGGA GAGCGGAACC CCACACTGCT TCCAGCCATG ATGCTGAAGA GGATGAGCTA 
CTGAAGGAGT CCCGTAGGAA GGAGGCCTTC TTCTGTCTGG AGTTCTTATT GGATAATGGT 
GCAGACCCCT CCCTGCGGGA CAGGCAGGGC TACACAGCTG TGCACTATGC AGCCGCCTAC 
GGCAACAGAC AGAACCTCGA ACTGCTCTTA GAAATGTCCT TTAACTGCCT GGAGGATGTG 
GAGAGCACCA TTCCAGTCAG CCCTTTGCAC TTAGCTGCCT ACAATGGTCA CTGTGAAGCT 
CTGAAGACAC TGGCCGAGAC GCTGGTGAAT CTGGATGTAA GGGACCACAA GGGCCGAACT 
GCGCTCTTCC TAGCCACTGA GCGAGGCTCT ACTGAGTGTG TGGAGGTGCT TACAGCCCAT 
GGCGCTTCTG CCCTCATCAA GGAGCGGAAG CGCAAGTGGA CACCCCTACA TGCTGCTGCT 
GCCTCTGGCC ACACTGATTC CCTGCACTTG CTGATTGACA GTGGGGAACG AGCTGACATC 
ACAGATGTCA TGGATGCCTA TGGACAAACC CCACTGATGC TAGCCATCAT GAATGGCCAT 
GTGGACTGTG TACATCTGCT GCTAGAGAAA GGATCCACAG CTGATGCTGC TGACCTCCGG 
GGCCGAACTG CCCTTCACCG TGGGGCAGTA ACTGGCTGTG AGGACTGCCT GGCCGCCCTG 
CTGGACCATG ATGCATTTGT ACTGTGCCGA GACTTTAAGG GCCGAACACC CATTCACCTG 
GCCTCAGCAT GTGGCCATAC TGCAGTGCTG CGGACTCTAC TGCAGGCTGC CCTTTCCACA 
GACCCCCTGG ATGCTGGCGT AGATTACAGC GGATACTCGC CCATGCACTG GGCCTCCTAC 
ACTGGACATG AAGATTGTCT GGAGTTGTTA CTTGAACACA GCCCATTCTC ATACTTGGAG 
GGAAACCCCT TCACTCCTTT GCACTGTGCA GTAATTAATA ACCAGGACAG CACCACAGAG 
ATGTTGCTGG GAGCTCTGGG TGCCAAGATT GTGAACAGCC GAGATGCCAA AGGACGGACC 
CCCCTTCACG CCGCTGCCTT TGCGGACAAT GTGTCTGGGC TCCGGATGCT GCTGCAGCAT 
CAAGCTGAGG TGAATGCCAC TGACCACACT GGCCGCACTG CACTCATGAC GGCAGCTGAG 
AATGGGCAGA CCGCTGCTGT GGAATTTCTG TTGTATCGAG GGAAGGCAGA CCTGACTGTG 
CTGGATGAGA ACAAGAACAC TGCCCTCCAC TTGGCTTGTA GCAAGGGCCA TGAGAAATGT 
GCCCTCATGA TCCTGGCAGA AACTCAAGAC CTTGGCCTTA TCAATGCTAC CAACAGTGCG 
CTGCAGATGC CACTCCACAT TGCTGCCCGG AATGGTTTAG CTTCTGTGGT GCAGGCCCTG 
CTAAGTCGTG GGGCCACAGT GCTGGCTGTG GATGAAGAAG GTCACACCCC AGCACTGGCC 
TGTGCTCCCA ACAAAGATGT GGCAGACTGC CTGGCCTTGA TCCTTTCCAC CATGAAGCCT 
TTCCCACCCA AGGACGCCGT CAGTCCTTTC AGCTTCAGCC TGCTCAAGAA CTGCGGCATC 
GCAGCTGCCA AGACGGTGGG TGGCTGTGGC GCCCTGCCCC ACGGGGCGTC CTGCCCCTAT 
AGCCAGGAAC GGCATGGCGC CATTGGGTTA GATGGCTGCT ACTCTGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd52 ( XM_017802080.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017657569.1 Nannospalax galili ankyrin repeat domain 52 (Ankrd52), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONa31326
Clone ID Related Accession (Same CDS sequence)	XM_017802080.1
Accession Version	XM_017802080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008351698.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATCC TCAGCATCAC AGACCAGCCG CCCCTGGTCC AGGCCATCTT TAGCCGAGAT 
GTGGAGGAAG TGCGTTCCCT CCTCTCGCAG AAGGAGAACA TCAATGTGCT GGACCAAGAG 
AGGCGAACCC CATTGCATGC TGCTGCCTAC GTAGGCGATG TCCCCATCCT CCAGTTGCTA 
CTGATGTCAG GTGCTAATGT CAACGCTAAG GACACACTGT GGCTGACCCC TCTTCATCGT 
GCTGCTGCCT CCCGAAACGA GAAGGTGCTG GGGCTGCTGC TGGCACATTC AGCTGATGTG 
AATGCCCGGG ACAAGCTGTG GCAGACACCA CTGCATGTGG CTGCTGCCAA CCGGGCCACC 
AAGTGTGCTG AGGCTCTGGC ACCCCTGTTG AGCAGCCTCA ATGTGGCTGA CAGGAGCGGG 
CGCAGTGCTC TACACCATGC AGTGCACAGT GGGCACCTTG AGACAGTGAA CCTGCTTCTC 
AACAAGGGAG CCAGCCTGAA TGTCTGCGAT AAAAAGGAGC GGCAGCCTCT GCACTGGGCA 
GCTTTTCTGG GGCATTTGGA GGTCCTGAAA CTGCTGGTGG CTCGTGGAGC AGACCTCGGC 
TGTAAGGACC GCAAGGGCTA TGGGCTGCTC CACACAGCCG CAGCCAGTGG CCAGATTGAA 
GTGGTGAAAT ACTTGCTCCG GATGGGGGCC GAGATTGATG AGCCCAATGC TTTTGGAAAC 
ACAGCTTTGC ACATTGCCTG CTACCTGGGC CAGGATGCTG TGGCTATTGA ACTGGTGAAT 
GCAGGAGCCA ATGTCAACCA GCCAAATGAC AAAGGCTTCA CACCACTGCA CGTGGCTGCG 
GTCTCCACAA ATGGCGCTCT CTGCTTGGAG CTGCTGGTCA ATAATGGGGC TGATGTCAAC 
TACCAGAGCA AAGAAGGGAA AAGTCCTCTG CACATGGCCG CCATCCACGG CCGTTTCACA 
CGCTCCCAGA TCCTCATTCA GAATGGTAGC GAGATTGATT GTGCTGACAA ATTTGGGAAC 
ACGCCACTAC ATGTGGCTGC TCGCTACGGA CACGAGCTGC TCATCAGCAC TCTCATGACC 
AATGGCGCGG ATACCGCCCG GCGCGGCATC CACGACATGT TCCCCCTGCA CTTAGCTGTT 
CTCTTTGGAT TCTCTGACTG TTGCCGGAAG CTTCTTTCCT CAGGTCAGCT GTACAGCATT 
GTGTCTTCAC TCAGCAATGA GCATGTGCTT TCCGCTGGGT TTGACATCAA CACACCTGAC 
AACCTTGGCC GCACCTGTCT TCATGCTGCT GCTTCTGGAG GGAATGTTGA ATGTCTTAAT 
TTGCTGTTGA GCAGTGGGGC TGACCTAAGA AGGAGAGACA AATTTGGAAG GACTCCACTG 
CACTATGCAG CGGCCAATGG TAGCTACCAG TGTGCAGTGA CATTGGTGAC TGCTGGGGCA 
GGCGTCAATG AGGCTGACTG TAAAGGCTGC TCTCCCCTCC ACTACGCTGC TGCCTCTGAT 
ACCTACCGGA GAGCGGAACC CCACACTGCT TCCAGCCATG ATGCTGAAGA GGATGAGCTA 
CTGAAGGAGT CCCGTAGGAA GGAGGCCTTC TTCTGTCTGG AGTTCTTATT GGATAATGGT 
GCAGACCCCT CCCTGCGGGA CAGGCAGGGC TACACAGCTG TGCACTATGC AGCCGCCTAC 
GGCAACAGAC AGAACCTCGA ACTGCTCTTA GAAATGTCCT TTAACTGCCT GGAGGATGTG 
GAGAGCACCA TTCCAGTCAG CCCTTTGCAC TTAGCTGCCT ACAATGGTCA CTGTGAAGCT 
CTGAAGACAC TGGCCGAGAC GCTGGTGAAT CTGGATGTAA GGGACCACAA GGGCCGAACT 
GCGCTCTTCC TAGCCACTGA GCGAGGCTCT ACTGAGTGTG TGGAGGTGCT TACAGCCCAT 
GGCGCTTCTG CCCTCATCAA GGAGCGGAAG CGCAAGTGGA CACCCCTACA TGCTGCTGCT 
GCCTCTGGCC ACACTGATTC CCTGCACTTG CTGATTGACA GTGGGGAACG AGCTGACATC 
ACAGATGTCA TGGATGCCTA TGGACAAACC CCACTGATGC TAGCCATCAT GAATGGCCAT 
GTGGACTGTG TACATCTGCT GCTAGAGAAA GGATCCACAG CTGATGCTGC TGACCTCCGG 
GGCCGAACTG CCCTTCACCG TGGGGCAGTA ACTGGCTGTG AGGACTGCCT GGCCGCCCTG 
CTGGACCATG ATGCATTTGT ACTGTGCCGA GACTTTAAGG GCCGAACACC CATTCACCTG 
GCCTCAGCAT GTGGCCATAC TGCAGTGCTG CGGACTCTAC TGCAGGCTGC CCTTTCCACA 
GACCCCCTGG ATGCTGGCGT AGATTACAGC GGATACTCGC CCATGCACTG GGCCTCCTAC 
ACTGGACATG AAGATTGTCT GGAGTTGTTA CTTGAACACA GCCCATTCTC ATACTTGGAG 
GGAAACCCCT TCACTCCTTT GCACTGTGCA GTAATTAATA ACCAGGACAG CACCACAGAG 
ATGTTGCTGG GAGCTCTGGG TGCCAAGATT GTGAACAGCC GAGATGCCAA AGGACGGACC 
CCCCTTCACG CCGCTGCCTT TGCGGACAAT GTGTCTGGGC TCCGGATGCT GCTGCAGCAT 
CAAGCTGAGG TGAATGCCAC TGACCACACT GGCCGCACTG CACTCATGAC GGCAGCTGAG 
AATGGGCAGA CCGCTGCTGT GGACCTGACT GTGCTGGATG AGAACAAGAA CACTGCCCTC 
CACTTGGCTT GTAGCAAGGG CCATGAGAAA TGTGCCCTCA TGATCCTGGC AGAAACTCAA 
GACCTTGGCC TTATCAATGC TACCAACAGT GCGCTGCAGA TGCCACTCCA CATTGCTGCC 
CGGAATGGTT TAGCTTCTGT GGTGCAGGCC CTGCTAAGTC GTGGGGCCAC AGTGCTGGCT 
GTGGATGAAG AAGGTCACAC CCCAGCACTG GCCTGTGCTC CCAACAAAGA TGTGGCAGAC 
TGCCTGGCCT TGATCCTTTC CACCATGAAG CCTTTCCCAC CCAAGGACGC CGTCAGTCCT 
TTCAGCTTCA GCCTGCTCAA GAACTGCGGC ATCGCAGCTG CCAAGACGGT GGGTGGCTGT 
GGCGCCCTGC CCCACGGGGC GTCCTGCCCC TATAGCCAGG AACGGCATGG CGCCATTGGG 
TTAGATGGCT GCTACTCTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017657569.1
CDS	82..3285
Translation	MGILSITDQPPLVQAIFSRDVEEVRSLLSQKENINVLDQERRTPLHAAAYVGDVPILQLLLMSGANVNAKDTLWLTPLHRAAASRNEKVLGLLLAHSADVNARDKLWQTPLHVAAANRATKCAEALAPLLSSLNVADRSGRSALHHAVHSGHLETVNLLLNKGASLNVCDKKERQPLHWAAFLGHLEVLKLLVARGADLGCKDRKGYGLLHTAAASGQIEVVKYLLRMGAEIDEPNAFGNTALHIACYLGQDAVAIELVNAGANVNQPNDKGFTPLHVAAVSTNGALCLELLVNNGADVNYQSKEGKSPLHMAAIHGRFTRSQILIQNGSEIDCADKFGNTPLHVAARYGHELLISTLMTNGADTARRGIHDMFPLHLAVLFGFSDCCRKLLSSGQLYSIVSSLSNEHVLSAGFDINTPDNLGRTCLHAAASGGNVECLNLLLSSGADLRRRDKFGRTPLHYAAANGSYQCAVTLVTAGAGVNEADCKGCSPLHYAAASDTYRRAEPHTASSHDAEEDELLKESRRKEAFFCLEFLLDNGADPSLRDRQGYTAVHYAAAYGNRQNLELLLEMSFNCLEDVESTIPVSPLHLAAYNGHCEALKTLAETLVNLDVRDHKGRTALFLATERGSTECVEVLTAHGASALIKERKRKWTPLHAAAASGHTDSLHLLIDSGERADITDVMDAYGQTPLMLAIMNGHVDCVHLLLEKGSTADAADLRGRTALHRGAVTGCEDCLAALLDHDAFVLCRDFKGRTPIHLASACGHTAVLRTLLQAALSTDPLDAGVDYSGYSPMHWASYTGHEDCLELLLEHSPFSYLEGNPFTPLHCAVINNQDSTTEMLLGALGAKIVNSRDAKGRTPLHAAAFADNVSGLRMLLQHQAEVNATDHTGRTALMTAAENGQTAAVDLTVLDENKNTALHLACSKGHEKCALMILAETQDLGLINATNSALQMPLHIAARNGLASVVQALLSRGATVLAVDEEGHTPALACAPNKDVADCLALILSTMKPFPPKDAVSPFSFSLLKNCGIAAAKTVGGCGALPHGASCPYSQERHGAIGLDGCYSE

Target ORF information:

RefSeq Version	XM_017802080.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili ankyrin repeat domain 52 (Ankrd52), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017802080.1

ORF Insert Sequence:

ATGGGGATCC TCAGCATCAC AGACCAGCCG CCCCTGGTCC AGGCCATCTT TAGCCGAGAT 
GTGGAGGAAG TGCGTTCCCT CCTCTCGCAG AAGGAGAACA TCAATGTGCT GGACCAAGAG 
AGGCGAACCC CATTGCATGC TGCTGCCTAC GTAGGCGATG TCCCCATCCT CCAGTTGCTA 
CTGATGTCAG GTGCTAATGT CAACGCTAAG GACACACTGT GGCTGACCCC TCTTCATCGT 
GCTGCTGCCT CCCGAAACGA GAAGGTGCTG GGGCTGCTGC TGGCACATTC AGCTGATGTG 
AATGCCCGGG ACAAGCTGTG GCAGACACCA CTGCATGTGG CTGCTGCCAA CCGGGCCACC 
AAGTGTGCTG AGGCTCTGGC ACCCCTGTTG AGCAGCCTCA ATGTGGCTGA CAGGAGCGGG 
CGCAGTGCTC TACACCATGC AGTGCACAGT GGGCACCTTG AGACAGTGAA CCTGCTTCTC 
AACAAGGGAG CCAGCCTGAA TGTCTGCGAT AAAAAGGAGC GGCAGCCTCT GCACTGGGCA 
GCTTTTCTGG GGCATTTGGA GGTCCTGAAA CTGCTGGTGG CTCGTGGAGC AGACCTCGGC 
TGTAAGGACC GCAAGGGCTA TGGGCTGCTC CACACAGCCG CAGCCAGTGG CCAGATTGAA 
GTGGTGAAAT ACTTGCTCCG GATGGGGGCC GAGATTGATG AGCCCAATGC TTTTGGAAAC 
ACAGCTTTGC ACATTGCCTG CTACCTGGGC CAGGATGCTG TGGCTATTGA ACTGGTGAAT 
GCAGGAGCCA ATGTCAACCA GCCAAATGAC AAAGGCTTCA CACCACTGCA CGTGGCTGCG 
GTCTCCACAA ATGGCGCTCT CTGCTTGGAG CTGCTGGTCA ATAATGGGGC TGATGTCAAC 
TACCAGAGCA AAGAAGGGAA AAGTCCTCTG CACATGGCCG CCATCCACGG CCGTTTCACA 
CGCTCCCAGA TCCTCATTCA GAATGGTAGC GAGATTGATT GTGCTGACAA ATTTGGGAAC 
ACGCCACTAC ATGTGGCTGC TCGCTACGGA CACGAGCTGC TCATCAGCAC TCTCATGACC 
AATGGCGCGG ATACCGCCCG GCGCGGCATC CACGACATGT TCCCCCTGCA CTTAGCTGTT 
CTCTTTGGAT TCTCTGACTG TTGCCGGAAG CTTCTTTCCT CAGGTCAGCT GTACAGCATT 
GTGTCTTCAC TCAGCAATGA GCATGTGCTT TCCGCTGGGT TTGACATCAA CACACCTGAC 
AACCTTGGCC GCACCTGTCT TCATGCTGCT GCTTCTGGAG GGAATGTTGA ATGTCTTAAT 
TTGCTGTTGA GCAGTGGGGC TGACCTAAGA AGGAGAGACA AATTTGGAAG GACTCCACTG 
CACTATGCAG CGGCCAATGG TAGCTACCAG TGTGCAGTGA CATTGGTGAC TGCTGGGGCA 
GGCGTCAATG AGGCTGACTG TAAAGGCTGC TCTCCCCTCC ACTACGCTGC TGCCTCTGAT 
ACCTACCGGA GAGCGGAACC CCACACTGCT TCCAGCCATG ATGCTGAAGA GGATGAGCTA 
CTGAAGGAGT CCCGTAGGAA GGAGGCCTTC TTCTGTCTGG AGTTCTTATT GGATAATGGT 
GCAGACCCCT CCCTGCGGGA CAGGCAGGGC TACACAGCTG TGCACTATGC AGCCGCCTAC 
GGCAACAGAC AGAACCTCGA ACTGCTCTTA GAAATGTCCT TTAACTGCCT GGAGGATGTG 
GAGAGCACCA TTCCAGTCAG CCCTTTGCAC TTAGCTGCCT ACAATGGTCA CTGTGAAGCT 
CTGAAGACAC TGGCCGAGAC GCTGGTGAAT CTGGATGTAA GGGACCACAA GGGCCGAACT 
GCGCTCTTCC TAGCCACTGA GCGAGGCTCT ACTGAGTGTG TGGAGGTGCT TACAGCCCAT 
GGCGCTTCTG CCCTCATCAA GGAGCGGAAG CGCAAGTGGA CACCCCTACA TGCTGCTGCT 
GCCTCTGGCC ACACTGATTC CCTGCACTTG CTGATTGACA GTGGGGAACG AGCTGACATC 
ACAGATGTCA TGGATGCCTA TGGACAAACC CCACTGATGC TAGCCATCAT GAATGGCCAT 
GTGGACTGTG TACATCTGCT GCTAGAGAAA GGATCCACAG CTGATGCTGC TGACCTCCGG 
GGCCGAACTG CCCTTCACCG TGGGGCAGTA ACTGGCTGTG AGGACTGCCT GGCCGCCCTG 
CTGGACCATG ATGCATTTGT ACTGTGCCGA GACTTTAAGG GCCGAACACC CATTCACCTG 
GCCTCAGCAT GTGGCCATAC TGCAGTGCTG CGGACTCTAC TGCAGGCTGC CCTTTCCACA 
GACCCCCTGG ATGCTGGCGT AGATTACAGC GGATACTCGC CCATGCACTG GGCCTCCTAC 
ACTGGACATG AAGATTGTCT GGAGTTGTTA CTTGAACACA GCCCATTCTC ATACTTGGAG 
GGAAACCCCT TCACTCCTTT GCACTGTGCA GTAATTAATA ACCAGGACAG CACCACAGAG 
ATGTTGCTGG GAGCTCTGGG TGCCAAGATT GTGAACAGCC GAGATGCCAA AGGACGGACC 
CCCCTTCACG CCGCTGCCTT TGCGGACAAT GTGTCTGGGC TCCGGATGCT GCTGCAGCAT 
CAAGCTGAGG TGAATGCCAC TGACCACACT GGCCGCACTG CACTCATGAC GGCAGCTGAG 
AATGGGCAGA CCGCTGCTGT GGACCTGACT GTGCTGGATG AGAACAAGAA CACTGCCCTC 
CACTTGGCTT GTAGCAAGGG CCATGAGAAA TGTGCCCTCA TGATCCTGGC AGAAACTCAA 
GACCTTGGCC TTATCAATGC TACCAACAGT GCGCTGCAGA TGCCACTCCA CATTGCTGCC 
CGGAATGGTT TAGCTTCTGT GGTGCAGGCC CTGCTAAGTC GTGGGGCCAC AGTGCTGGCT 
GTGGATGAAG AAGGTCACAC CCCAGCACTG GCCTGTGCTC CCAACAAAGA TGTGGCAGAC 
TGCCTGGCCT TGATCCTTTC CACCATGAAG CCTTTCCCAC CCAAGGACGC CGTCAGTCCT 
TTCAGCTTCA GCCTGCTCAA GAACTGCGGC ATCGCAGCTG CCAAGACGGT GGGTGGCTGT 
GGCGCCCTGC CCCACGGGGC GTCCTGCCCC TATAGCCAGG AACGGCATGG CGCCATTGGG 
TTAGATGGCT GCTACTCTGA GTAG