Ern1 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ern1
Entrez Gene ID	103744745
Full Name	endoplasmic reticulum to nucleus signaling 1
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008846579.2	XP_008844801.1	serine/threonine-protein kinase/endoribonuclease IRE1
XM_008846579.3	XP_008844801.1	serine/threonine-protein kinase/endoribonuclease IRE1

Pathways from BioSystems
Gene Ontology

The following Ern1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ern1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa29580	XM_008846579.3 Latest version!	Nannospalax galili endoplasmic reticulum to nucleus signaling 1 (Ern1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ONa29580	XM_008846579.2	Nannospalax galili endoplasmic reticulum to nucleus signaling 1 (Ern1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ern1 ( XM_008846579.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008844801.1 Nannospalax galili endoplasmic reticulum to nucleus signaling 1 (Ern1), mRNA.

Price & Availability↑

CloneID	ONa29580
Clone ID Related Accession (Same CDS sequence)	XM_008846579.2 , XM_008846579.3
Accession Version	XM_008846579.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	serine/threonine-protein kinase/endoribonuclease IRE1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008349234.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008846579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCCT GGCGACTGCC GCTCCTGCTG GCGCTGCTGC TGCTGCTGTC CGGCCCTGGG 
ATTTTTGGAA GTACCAGCAC AATAACACTG CCTGAAACCT TGTTGTTTGT TTCAACCCTG 
GATGGAAGTT TGCACGCTGT CAGCAAGAGG ACAGGCTCAA TCAAGTGGAC TTTAAAAGAA 
GATCCAGTCC TGCAGGTTCC GACACACGTG GAAGAGCCTG CCTTCCTCCC AGATCCCAAT 
GATGGTAGTC TGTACACACT TGGGGGCAAG AACAACGAAG GTCTGACGAA ACTTCCCTTT 
ACCATCCCAG AATTGGTCCA GGCATCCCCA TGCCGAAGTT CAGATGGAAT CCTCTACATG 
GGTAAAAAGC AGGACATTTG GTATGTCATT GACCTCCTGA CTGGCGAGAA GCAGCAGACT 
TTGTCATCAG CCTTTGCAGA TAGTCTCTGC CCCTCTACCT CCCTGCTGTA TCTTGGCCGG 
ACAGAGTACA CTATCACCAT GTATGACACA AAGACCCGTG AACTCCGCTG GAATGCCACC 
TATTTTGACT ACGCAGCCTC ACTGCCCGAG GACGACGTGG ACTACAAAAT GTCCCACTTT 
GTGTCCAATG GCGACGGACT GGTGGTAACT GTGGATAGTG AATCTGGGGA CGTCCTATGG 
ATCCAAAACT ATGCCTCTCC TGTGGTAGCC TTCTACGTCT GGCAGCGGGA GGGACTGAGG 
AAGGTGATAC ATATCAATGT TGCCGTGGAG ACTCTGCGCT ACTTGACCTT CATGTCTGGG 
GAAGTAGGGC GCATCACCAA GTGGAAGTAT CCATTCCCCA AAGAGACGGA GGCCAAGAGC 
AAGCTGACGC CTACCCTATA TGTTGGGAAG TACTCCACCA GCCTGTATGC CTCTCCCTCA 
ATGGTGCACG AGGGGGTCGC CGTTGTGCCC CGAGGCAGCA CTCTTCCTTT GCTGGAAGGC 
CCCCAGACCG ATGGCGTCAC CATTGGGGAC AAAGGGGAAT GTGTGATCAC GCCCAGCACA 
GACCTCAAGT TTAACCCTGG ACTGAAAGGG AAGAGCAAGC TGAACTACTT GAGAAATTAC 
TGGCTTCTCA TAGGACACCA TGAAACCCCA CTGTCTGCAT CTACCAAGAT GCTTGAGAGA 
TTTCCCAACA ATCTGCCCAA ACATCGGGAA AATGTGATTC CTGCTGATTC AGAAAAAAAG 
AGCTTTGAGG AAGTTATCAA CCTGGTTGAC CAGACTTCAG AAAACACACC AACCACTGTG 
TCTCAGGATG TAGAGGAGAA ACTGACTCGT GCCCCTGCCA AGCCTGAGGC CCCTGTGGAC 
TCCATGCTCA AGGATGTGGC CACCATCATC CTAAGCACGT TTCTGCTGGT TGGATGGGTA 
GCCTTCATCA TCACCTACCC CTTGAGCGTG CATCAGCAGC GCCAGCTCCA GCACCAGCAG 
TTCCAGAAGG AACTGGAGAA AATCCAGCTC CTGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CATGGAGACC TGACCCAAGA CCCTGAGTTC CTGGATACGT CTGGCCTCTT CTCAGAAAGC 
TCAGGCACCA GCAGCCCCAG CCCATCCCCC AGAGCTTCTA ACCACTCACT TCACTCCAGC 
AGCTCTGCTT CCAGGGCTGG CCCAAGCCCT TTCCTGGAGC AGGATGACGA GGATGAGGAA 
ACCAGAATGG TGATTGTTGG GAAAATTTCA TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGTA CAATTGTATA CAAGGGCATG TTTGACAACC GTGACGTGGC TGTGAAGAGG 
ATCCTCCCCG AGTGCTTTAG CTTTGCAGAC CGTGAGGTCC AGCTGCTTCG AGAATCCGAC 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACAGAGAAGG ACCGGCAGTT CCAGTACATT 
GCCATTGAGC TATGTGCAGC TACCCTGCAG GAGTATGTAG AGCAGAAGGA CTTTGCCCAC 
CTTGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CCGGCCTGGC ACACCTGCAC 
TCCCTCAACA TTGTTCACAG AGACCTGAAG CCCCACAACA TCCTCCTCTC CATGCCTAAT 
GCACATGGCA AGATCAAGGC TATGATCTCT GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGGCACA GTTTCAGCCG CCGCTCTGGG GTGCCTGGCA CTGAAGGCTG GATAGCCCCA 
GAGATGCTGA GTGAAGACTG CAAGGAGAAC CCTACCTACA CAGTGGACAT CTTCTCTGCA 
GGCTGTGTCT TCTACTATGT CATCTCTGAG GGTAGCCACC CTTTCGGCAA GTCCCTGCAG 
CGTCAGGCCA ACATCCTCCT GGGCGCCTGC AGCCTTGACT GCTTCTGCTC TGACAAACAC 
GAAGATGTCA TTGCTCGTGA GTTGATAGAG AAAATGATTT CGATGGACCC CCAACAGCGA 
CCATCTGCAA AGCGTGTGCT AAGACACCCA TTCTTCTGGA GCCTGGAAAA GCAGCTGCAG 
TTTTTTCAGG ACGTGAGTGA CCGGATAGAA AAGGAGTCCT TGGATGGCCC AATAGTGAAG 
CAGTTAGAGA GAGGTGGGAG AGCTGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTGCAGA CAGATCTGCG TAAATTCAGA ACCTACAAAG GTGGCTCCGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGAGAGC TCCCTGTGGA GGTTCAGGAG 
ACACTGGGTT CCCTCCCTGA TGACTTTGTG CGCTACTTCA CTTCGCGCTT CCCCCACCTC 
CTCTCTCACA CCTACCGGGC CATGGAGCTC TGCAGCCAGG AGAGACTCTT CCAGCCCTAC 
TACTTGCATG AGCCCACGGA GCCCCAGCCC CCAGTGACTC CAGATGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008844801.1
CDS	150..3083
Translation	MPAWRLPLLLALLLLLSGPGIFGSTSTITLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGGKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVIHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDLKFNPGLKGKSKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENTPTTVSQDVEEKLTRAPAKPEAPVDSMLKDVATIILSTFLLVGWVAFIITYPLSVHQQRQLQHQQFQKELEKIQLLQQQQLPFHPHGDLTQDPEFLDTSGLFSESSGTSSPSPSPRASNHSLHSSSSASRAGPSPFLEQDDEDEETRMVIVGKISFCPKDVLGHGAEGTIVYKGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILLSMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCFCSDKHEDVIARELIEKMISMDPQQRPSAKRVLRHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPVEVQETLGSLPDDFVRYFTSRFPHLLSHTYRAMELCSQERLFQPYYLHEPTEPQPPVTPDAL

Target ORF information:

RefSeq Version	XM_008846579.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili endoplasmic reticulum to nucleus signaling 1 (Ern1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008846579.2

ORF Insert Sequence:

ATGCCGGCCT GGCGACTGCC GCTCCTGCTG GCGCTGCTGC TGCTGCTGTC CGGCCCTGGG 
ATTTTTGGAA GTACCAGCAC AATAACACTG CCTGAAACCT TGTTGTTTGT TTCAACCCTG 
GATGGAAGTT TGCACGCTGT CAGCAAGAGG ACAGGCTCAA TCAAGTGGAC TTTAAAAGAA 
GATCCAGTCC TGCAGGTTCC GACACACGTG GAAGAGCCTG CCTTCCTCCC AGATCCCAAT 
GATGGTAGTC TGTACACACT TGGGGGCAAG AACAACGAAG GTCTGACGAA ACTTCCCTTT 
ACCATCCCAG AATTGGTCCA GGCATCCCCA TGCCGAAGTT CAGATGGAAT CCTCTACATG 
GGTAAAAAGC AGGACATTTG GTATGTCATT GACCTCCTGA CTGGCGAGAA GCAGCAGACT 
TTGTCATCAG CCTTTGCAGA TAGTCTCTGC CCCTCTACCT CCCTGCTGTA TCTTGGCCGG 
ACAGAGTACA CTATCACCAT GTATGACACA AAGACCCGTG AACTCCGCTG GAATGCCACC 
TATTTTGACT ACGCAGCCTC ACTGCCCGAG GACGACGTGG ACTACAAAAT GTCCCACTTT 
GTGTCCAATG GCGACGGACT GGTGGTAACT GTGGATAGTG AATCTGGGGA CGTCCTATGG 
ATCCAAAACT ATGCCTCTCC TGTGGTAGCC TTCTACGTCT GGCAGCGGGA GGGACTGAGG 
AAGGTGATAC ATATCAATGT TGCCGTGGAG ACTCTGCGCT ACTTGACCTT CATGTCTGGG 
GAAGTAGGGC GCATCACCAA GTGGAAGTAT CCATTCCCCA AAGAGACGGA GGCCAAGAGC 
AAGCTGACGC CTACCCTATA TGTTGGGAAG TACTCCACCA GCCTGTATGC CTCTCCCTCA 
ATGGTGCACG AGGGGGTCGC CGTTGTGCCC CGAGGCAGCA CTCTTCCTTT GCTGGAAGGC 
CCCCAGACCG ATGGCGTCAC CATTGGGGAC AAAGGGGAAT GTGTGATCAC GCCCAGCACA 
GACCTCAAGT TTAACCCTGG ACTGAAAGGG AAGAGCAAGC TGAACTACTT GAGAAATTAC 
TGGCTTCTCA TAGGACACCA TGAAACCCCA CTGTCTGCAT CTACCAAGAT GCTTGAGAGA 
TTTCCCAACA ATCTGCCCAA ACATCGGGAA AATGTGATTC CTGCTGATTC AGAAAAAAAG 
AGCTTTGAGG AAGTTATCAA CCTGGTTGAC CAGACTTCAG AAAACACACC AACCACTGTG 
TCTCAGGATG TAGAGGAGAA ACTGACTCGT GCCCCTGCCA AGCCTGAGGC CCCTGTGGAC 
TCCATGCTCA AGGATGTGGC CACCATCATC CTAAGCACGT TTCTGCTGGT TGGATGGGTA 
GCCTTCATCA TCACCTACCC CTTGAGCGTG CATCAGCAGC GCCAGCTCCA GCACCAGCAG 
TTCCAGAAGG AACTGGAGAA AATCCAGCTC CTGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CATGGAGACC TGACCCAAGA CCCTGAGTTC CTGGATACGT CTGGCCTCTT CTCAGAAAGC 
TCAGGCACCA GCAGCCCCAG CCCATCCCCC AGAGCTTCTA ACCACTCACT TCACTCCAGC 
AGCTCTGCTT CCAGGGCTGG CCCAAGCCCT TTCCTGGAGC AGGATGACGA GGATGAGGAA 
ACCAGAATGG TGATTGTTGG GAAAATTTCA TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGTA CAATTGTATA CAAGGGCATG TTTGACAACC GTGACGTGGC TGTGAAGAGG 
ATCCTCCCCG AGTGCTTTAG CTTTGCAGAC CGTGAGGTCC AGCTGCTTCG AGAATCCGAC 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACAGAGAAGG ACCGGCAGTT CCAGTACATT 
GCCATTGAGC TATGTGCAGC TACCCTGCAG GAGTATGTAG AGCAGAAGGA CTTTGCCCAC 
CTTGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CCGGCCTGGC ACACCTGCAC 
TCCCTCAACA TTGTTCACAG AGACCTGAAG CCCCACAACA TCCTCCTCTC CATGCCTAAT 
GCACATGGCA AGATCAAGGC TATGATCTCT GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGGCACA GTTTCAGCCG CCGCTCTGGG GTGCCTGGCA CTGAAGGCTG GATAGCCCCA 
GAGATGCTGA GTGAAGACTG CAAGGAGAAC CCTACCTACA CAGTGGACAT CTTCTCTGCA 
GGCTGTGTCT TCTACTATGT CATCTCTGAG GGTAGCCACC CTTTCGGCAA GTCCCTGCAG 
CGTCAGGCCA ACATCCTCCT GGGCGCCTGC AGCCTTGACT GCTTCTGCTC TGACAAACAC 
GAAGATGTCA TTGCTCGTGA GTTGATAGAG AAAATGATTT CGATGGACCC CCAACAGCGA 
CCATCTGCAA AGCGTGTGCT AAGACACCCA TTCTTCTGGA GCCTGGAAAA GCAGCTGCAG 
TTTTTTCAGG ACGTGAGTGA CCGGATAGAA AAGGAGTCCT TGGATGGCCC AATAGTGAAG 
CAGTTAGAGA GAGGTGGGAG AGCTGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTGCAGA CAGATCTGCG TAAATTCAGA ACCTACAAAG GTGGCTCCGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGAGAGC TCCCTGTGGA GGTTCAGGAG 
ACACTGGGTT CCCTCCCTGA TGACTTTGTG CGCTACTTCA CTTCGCGCTT CCCCCACCTC 
CTCTCTCACA CCTACCGGGC CATGGAGCTC TGCAGCCAGG AGAGACTCTT CCAGCCCTAC 
TACTTGCATG AGCCCACGGA GCCCCAGCCC CCAGTGACTC CAGATGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ern1 ( XM_008846579.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008844801.1 Nannospalax galili endoplasmic reticulum to nucleus signaling 1 (Ern1), mRNA.

Price & Availability↑

CloneID	ONa29580
Clone ID Related Accession (Same CDS sequence)	XM_008846579.2 , XM_008846579.3
Accession Version	XM_008846579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	serine/threonine-protein kinase/endoribonuclease IRE1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008349234.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008846579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCCT GGCGACTGCC GCTCCTGCTG GCGCTGCTGC TGCTGCTGTC CGGCCCTGGG 
ATTTTTGGAA GTACCAGCAC AATAACACTG CCTGAAACCT TGTTGTTTGT TTCAACCCTG 
GATGGAAGTT TGCACGCTGT CAGCAAGAGG ACAGGCTCAA TCAAGTGGAC TTTAAAAGAA 
GATCCAGTCC TGCAGGTTCC GACACACGTG GAAGAGCCTG CCTTCCTCCC AGATCCCAAT 
GATGGTAGTC TGTACACACT TGGGGGCAAG AACAACGAAG GTCTGACGAA ACTTCCCTTT 
ACCATCCCAG AATTGGTCCA GGCATCCCCA TGCCGAAGTT CAGATGGAAT CCTCTACATG 
GGTAAAAAGC AGGACATTTG GTATGTCATT GACCTCCTGA CTGGCGAGAA GCAGCAGACT 
TTGTCATCAG CCTTTGCAGA TAGTCTCTGC CCCTCTACCT CCCTGCTGTA TCTTGGCCGG 
ACAGAGTACA CTATCACCAT GTATGACACA AAGACCCGTG AACTCCGCTG GAATGCCACC 
TATTTTGACT ACGCAGCCTC ACTGCCCGAG GACGACGTGG ACTACAAAAT GTCCCACTTT 
GTGTCCAATG GCGACGGACT GGTGGTAACT GTGGATAGTG AATCTGGGGA CGTCCTATGG 
ATCCAAAACT ATGCCTCTCC TGTGGTAGCC TTCTACGTCT GGCAGCGGGA GGGACTGAGG 
AAGGTGATAC ATATCAATGT TGCCGTGGAG ACTCTGCGCT ACTTGACCTT CATGTCTGGG 
GAAGTAGGGC GCATCACCAA GTGGAAGTAT CCATTCCCCA AAGAGACGGA GGCCAAGAGC 
AAGCTGACGC CTACCCTATA TGTTGGGAAG TACTCCACCA GCCTGTATGC CTCTCCCTCA 
ATGGTGCACG AGGGGGTCGC CGTTGTGCCC CGAGGCAGCA CTCTTCCTTT GCTGGAAGGC 
CCCCAGACCG ATGGCGTCAC CATTGGGGAC AAAGGGGAAT GTGTGATCAC GCCCAGCACA 
GACCTCAAGT TTAACCCTGG ACTGAAAGGG AAGAGCAAGC TGAACTACTT GAGAAATTAC 
TGGCTTCTCA TAGGACACCA TGAAACCCCA CTGTCTGCAT CTACCAAGAT GCTTGAGAGA 
TTTCCCAACA ATCTGCCCAA ACATCGGGAA AATGTGATTC CTGCTGATTC AGAAAAAAAG 
AGCTTTGAGG AAGTTATCAA CCTGGTTGAC CAGACTTCAG AAAACACACC AACCACTGTG 
TCTCAGGATG TAGAGGAGAA ACTGACTCGT GCCCCTGCCA AGCCTGAGGC CCCTGTGGAC 
TCCATGCTCA AGGATGTGGC CACCATCATC CTAAGCACGT TTCTGCTGGT TGGATGGGTA 
GCCTTCATCA TCACCTACCC CTTGAGCGTG CATCAGCAGC GCCAGCTCCA GCACCAGCAG 
TTCCAGAAGG AACTGGAGAA AATCCAGCTC CTGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CATGGAGACC TGACCCAAGA CCCTGAGTTC CTGGATACGT CTGGCCTCTT CTCAGAAAGC 
TCAGGCACCA GCAGCCCCAG CCCATCCCCC AGAGCTTCTA ACCACTCACT TCACTCCAGC 
AGCTCTGCTT CCAGGGCTGG CCCAAGCCCT TTCCTGGAGC AGGATGACGA GGATGAGGAA 
ACCAGAATGG TGATTGTTGG GAAAATTTCA TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGTA CAATTGTATA CAAGGGCATG TTTGACAACC GTGACGTGGC TGTGAAGAGG 
ATCCTCCCCG AGTGCTTTAG CTTTGCAGAC CGTGAGGTCC AGCTGCTTCG AGAATCCGAC 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACAGAGAAGG ACCGGCAGTT CCAGTACATT 
GCCATTGAGC TATGTGCAGC TACCCTGCAG GAGTATGTAG AGCAGAAGGA CTTTGCCCAC 
CTTGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CCGGCCTGGC ACACCTGCAC 
TCCCTCAACA TTGTTCACAG AGACCTGAAG CCCCACAACA TCCTCCTCTC CATGCCTAAT 
GCACATGGCA AGATCAAGGC TATGATCTCT GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGGCACA GTTTCAGCCG CCGCTCTGGG GTGCCTGGCA CTGAAGGCTG GATAGCCCCA 
GAGATGCTGA GTGAAGACTG CAAGGAGAAC CCTACCTACA CAGTGGACAT CTTCTCTGCA 
GGCTGTGTCT TCTACTATGT CATCTCTGAG GGTAGCCACC CTTTCGGCAA GTCCCTGCAG 
CGTCAGGCCA ACATCCTCCT GGGCGCCTGC AGCCTTGACT GCTTCTGCTC TGACAAACAC 
GAAGATGTCA TTGCTCGTGA GTTGATAGAG AAAATGATTT CGATGGACCC CCAACAGCGA 
CCATCTGCAA AGCGTGTGCT AAGACACCCA TTCTTCTGGA GCCTGGAAAA GCAGCTGCAG 
TTTTTTCAGG ACGTGAGTGA CCGGATAGAA AAGGAGTCCT TGGATGGCCC AATAGTGAAG 
CAGTTAGAGA GAGGTGGGAG AGCTGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTGCAGA CAGATCTGCG TAAATTCAGA ACCTACAAAG GTGGCTCCGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGAGAGC TCCCTGTGGA GGTTCAGGAG 
ACACTGGGTT CCCTCCCTGA TGACTTTGTG CGCTACTTCA CTTCGCGCTT CCCCCACCTC 
CTCTCTCACA CCTACCGGGC CATGGAGCTC TGCAGCCAGG AGAGACTCTT CCAGCCCTAC 
TACTTGCATG AGCCCACGGA GCCCCAGCCC CCAGTGACTC CAGATGCCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008844801.1
CDS	194..3127
Translation	MPAWRLPLLLALLLLLSGPGIFGSTSTITLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGGKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVIHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDLKFNPGLKGKSKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENTPTTVSQDVEEKLTRAPAKPEAPVDSMLKDVATIILSTFLLVGWVAFIITYPLSVHQQRQLQHQQFQKELEKIQLLQQQQLPFHPHGDLTQDPEFLDTSGLFSESSGTSSPSPSPRASNHSLHSSSSASRAGPSPFLEQDDEDEETRMVIVGKISFCPKDVLGHGAEGTIVYKGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILLSMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCFCSDKHEDVIARELIEKMISMDPQQRPSAKRVLRHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPVEVQETLGSLPDDFVRYFTSRFPHLLSHTYRAMELCSQERLFQPYYLHEPTEPQPPVTPDAL

Target ORF information:

RefSeq Version	XM_008846579.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili endoplasmic reticulum to nucleus signaling 1 (Ern1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008846579.3

ORF Insert Sequence:

ATGCCGGCCT GGCGACTGCC GCTCCTGCTG GCGCTGCTGC TGCTGCTGTC CGGCCCTGGG 
ATTTTTGGAA GTACCAGCAC AATAACACTG CCTGAAACCT TGTTGTTTGT TTCAACCCTG 
GATGGAAGTT TGCACGCTGT CAGCAAGAGG ACAGGCTCAA TCAAGTGGAC TTTAAAAGAA 
GATCCAGTCC TGCAGGTTCC GACACACGTG GAAGAGCCTG CCTTCCTCCC AGATCCCAAT 
GATGGTAGTC TGTACACACT TGGGGGCAAG AACAACGAAG GTCTGACGAA ACTTCCCTTT 
ACCATCCCAG AATTGGTCCA GGCATCCCCA TGCCGAAGTT CAGATGGAAT CCTCTACATG 
GGTAAAAAGC AGGACATTTG GTATGTCATT GACCTCCTGA CTGGCGAGAA GCAGCAGACT 
TTGTCATCAG CCTTTGCAGA TAGTCTCTGC CCCTCTACCT CCCTGCTGTA TCTTGGCCGG 
ACAGAGTACA CTATCACCAT GTATGACACA AAGACCCGTG AACTCCGCTG GAATGCCACC 
TATTTTGACT ACGCAGCCTC ACTGCCCGAG GACGACGTGG ACTACAAAAT GTCCCACTTT 
GTGTCCAATG GCGACGGACT GGTGGTAACT GTGGATAGTG AATCTGGGGA CGTCCTATGG 
ATCCAAAACT ATGCCTCTCC TGTGGTAGCC TTCTACGTCT GGCAGCGGGA GGGACTGAGG 
AAGGTGATAC ATATCAATGT TGCCGTGGAG ACTCTGCGCT ACTTGACCTT CATGTCTGGG 
GAAGTAGGGC GCATCACCAA GTGGAAGTAT CCATTCCCCA AAGAGACGGA GGCCAAGAGC 
AAGCTGACGC CTACCCTATA TGTTGGGAAG TACTCCACCA GCCTGTATGC CTCTCCCTCA 
ATGGTGCACG AGGGGGTCGC CGTTGTGCCC CGAGGCAGCA CTCTTCCTTT GCTGGAAGGC 
CCCCAGACCG ATGGCGTCAC CATTGGGGAC AAAGGGGAAT GTGTGATCAC GCCCAGCACA 
GACCTCAAGT TTAACCCTGG ACTGAAAGGG AAGAGCAAGC TGAACTACTT GAGAAATTAC 
TGGCTTCTCA TAGGACACCA TGAAACCCCA CTGTCTGCAT CTACCAAGAT GCTTGAGAGA 
TTTCCCAACA ATCTGCCCAA ACATCGGGAA AATGTGATTC CTGCTGATTC AGAAAAAAAG 
AGCTTTGAGG AAGTTATCAA CCTGGTTGAC CAGACTTCAG AAAACACACC AACCACTGTG 
TCTCAGGATG TAGAGGAGAA ACTGACTCGT GCCCCTGCCA AGCCTGAGGC CCCTGTGGAC 
TCCATGCTCA AGGATGTGGC CACCATCATC CTAAGCACGT TTCTGCTGGT TGGATGGGTA 
GCCTTCATCA TCACCTACCC CTTGAGCGTG CATCAGCAGC GCCAGCTCCA GCACCAGCAG 
TTCCAGAAGG AACTGGAGAA AATCCAGCTC CTGCAGCAGC AGCAGCTGCC CTTCCACCCA 
CATGGAGACC TGACCCAAGA CCCTGAGTTC CTGGATACGT CTGGCCTCTT CTCAGAAAGC 
TCAGGCACCA GCAGCCCCAG CCCATCCCCC AGAGCTTCTA ACCACTCACT TCACTCCAGC 
AGCTCTGCTT CCAGGGCTGG CCCAAGCCCT TTCCTGGAGC AGGATGACGA GGATGAGGAA 
ACCAGAATGG TGATTGTTGG GAAAATTTCA TTCTGTCCCA AGGATGTCCT GGGCCATGGA 
GCTGAGGGTA CAATTGTATA CAAGGGCATG TTTGACAACC GTGACGTGGC TGTGAAGAGG 
ATCCTCCCCG AGTGCTTTAG CTTTGCAGAC CGTGAGGTCC AGCTGCTTCG AGAATCCGAC 
GAGCACCCGA ACGTGATCCG CTACTTCTGC ACAGAGAAGG ACCGGCAGTT CCAGTACATT 
GCCATTGAGC TATGTGCAGC TACCCTGCAG GAGTATGTAG AGCAGAAGGA CTTTGCCCAC 
CTTGGCCTGG AGCCCATCAC CTTGCTGCAG CAGACCACCT CCGGCCTGGC ACACCTGCAC 
TCCCTCAACA TTGTTCACAG AGACCTGAAG CCCCACAACA TCCTCCTCTC CATGCCTAAT 
GCACATGGCA AGATCAAGGC TATGATCTCT GACTTTGGCC TCTGCAAGAA GCTGGCAGTG 
GGCAGGCACA GTTTCAGCCG CCGCTCTGGG GTGCCTGGCA CTGAAGGCTG GATAGCCCCA 
GAGATGCTGA GTGAAGACTG CAAGGAGAAC CCTACCTACA CAGTGGACAT CTTCTCTGCA 
GGCTGTGTCT TCTACTATGT CATCTCTGAG GGTAGCCACC CTTTCGGCAA GTCCCTGCAG 
CGTCAGGCCA ACATCCTCCT GGGCGCCTGC AGCCTTGACT GCTTCTGCTC TGACAAACAC 
GAAGATGTCA TTGCTCGTGA GTTGATAGAG AAAATGATTT CGATGGACCC CCAACAGCGA 
CCATCTGCAA AGCGTGTGCT AAGACACCCA TTCTTCTGGA GCCTGGAAAA GCAGCTGCAG 
TTTTTTCAGG ACGTGAGTGA CCGGATAGAA AAGGAGTCCT TGGATGGCCC AATAGTGAAG 
CAGTTAGAGA GAGGTGGGAG AGCTGTGGTG AAGATGGACT GGCGGGAGAA CATCACTGTC 
CCCCTGCAGA CAGATCTGCG TAAATTCAGA ACCTACAAAG GTGGCTCCGT CAGAGATCTC 
CTCCGAGCCA TGAGAAATAA GAAGCACCAC TACCGAGAGC TCCCTGTGGA GGTTCAGGAG 
ACACTGGGTT CCCTCCCTGA TGACTTTGTG CGCTACTTCA CTTCGCGCTT CCCCCACCTC 
CTCTCTCACA CCTACCGGGC CATGGAGCTC TGCAGCCAGG AGAGACTCTT CCAGCCCTAC 
TACTTGCATG AGCCCACGGA GCCCCAGCCC CCAGTGACTC CAGATGCCCT CTGA