Bub1b cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bub1b
Entrez Gene ID	103741499
Full Name	BUB1 mitotic checkpoint serine/threonine kinase B
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008842313.3	XP_008840535.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
XM_008842313.2	XP_008840535.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Pathways from BioSystems
Gene Ontology

The following Bub1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bub1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa24872	XM_008842313.3 Latest version!	Nannospalax galili BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
ONa24872	XM_008842313.2	Nannospalax galili BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bub1b ( XM_008842313.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008840535.1 Nannospalax galili BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), mRNA.

Price & Availability↑

CloneID	ONa24872
Clone ID Related Accession (Same CDS sequence)	XM_008842313.3 , XM_008842313.2
Accession Version	XM_008842313.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008345995.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008842313.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCAAGAAGGA AGGGCTCGCC GTGAGTGAAG CTATGTCCCT GGAGGGAGAT 
GAGTGGGAGC TGAGTAAAGA AAATGTACAA CCCTTAAGGC ATGGGCGGAT CATGTCTACA 
CTTCAGGGAG CTTTGGCACA GCAGGAATCG GCCTGTAACA CTACTCTTCA GCAACAGAAA 
CGGGCATTTG AATCTGAAAT TCGCTTTTAC GCTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGGTATATTA AGTGGACAGA ACAGAACTAT CCTCAAGGGG GCAAGGAGAG CAACATGTCA 
ACATTACTCG AAAGAGCTAT AGAAGCTCTA CAAGGAGAAA AACGTTATTA TAGTGACCCT 
CGGTTTCTCA GTCTCTGGCT CAAATTGGGG CATTTGTGCA ATGAGCCATT GGACATGTAC 
AGTTATTTAC ACAGCCAAGG GATTGGCGTT TCACTTGCTC AGTTCTATAT TTCGTGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCTGATA CGGTATTTCA GGAAGGGATT 
CAACGGAAGG CCGAGCCACT AGAAAGACTG CAGTCCCAGC ACCGACAATT CCAGGCTCGG 
GTGTCTCGAC AAGCTCTCTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TTTAGGATCT 
TCCATTCCAC AGCGAAGCAC ATTAGCTGAG CTAAAGAGCA AAGGGAAGAA GACAGCAAAA 
GCTCCCATTA GCCGTGTAGG AGGTGCTCTG AAGGCTCCAG GTCAGAGCAG AGGATTCCAA 
AATATAGTTC CTCAGCAGAT GCAAAGTAAT CGCAGAATTA CTGTTTTTGA TGAAAATTCT 
GATATCACTT CTAGAGCAGA GTTATCTAAG CCCGAAGTCC AGCCCTGGAT GGCACCCCCT 
GTTCCAAGGG CCAAAGAGAA TGAGCTGCAG CCAGGCCCTT GGAACACAGG CAGGCCTTTG 
GAATACAGGC CTCATGGCAG TACAGCTTCT TTAACAGCTG TACCCCCTAT GCTTCCCAGC 
TTTACTCCAT ATGTGGAAGA GACTGCACAA CAACCAGTTA TGACACCATG TAAAATTGAA 
CCTAGTATAA ACCATATTCT AAGCACCAGA AAGCCTGGAA AGGAAGAAGG AGATCCTTTT 
CAAAGGGTTC AGAGTCATCA GCAAGAGTCT GAAGAAAAGA AAGAGAAGAT GATGTACTGT 
AAGGAGAAGA TTTATGCAGG AGTTGGGGAG TTCTCTTTTG AAGAAATCCG GGCTGAAGTT 
TTCCGGAAGA AATTAAAAGA GCGAAGGGAA GCGGAACTAC TGACCAGTGC AGAGAAGAGA 
GCAGAAATGC AGAAACAGAT TGAAGAGATG GAGAAAAAGC TCAAAGAAAT CCAGACTACT 
CAGCAGGAAA GAACTGGTGA TCAGCAAGAA GAGAAGATAC CTGCAAAGAC AGACAAACTG 
CAAATTGCTT CAGAGTCTCA GGAAATGACA GGAGTGTCTC TTTCCCATTC TGTTTGTCCA 
CTAAACTCTC ACCCCAGGAA AACTTTACCT ATAGAAAATG TTTTGCAAGA ACAGCCTGAT 
AGTAAAGGTT CCAGTATACC TTTCTCCATT TTTGATGAGT TTCTTGTTTC AGAAAAAAAG 
AATGAAAGTC CTACAGATCC TCCACGAGCT TTAGCACAGC GACAGCCTCT TGCAGTTTTA 
AAAACCACAG AAATAATTGC TTCAAATGAG GATATGTCTC CAGATGTTTG TGATGAACTT 
ACAGAAATTG AACCTTTGAG TGAGGATGCC ATTATCACAG GTTTCAGGAA TGTAACTATT 
TGTCCTAACC CAGAAGACAC ATGTGACTTC GCTAGAGCTG CTCATTTTGC CTCTACGCCT 
TTTCATGAGA TAATGTCCTT GAAGGATATC TCTTCCGATC CTGAGAGACT GCTGCAGGAA 
GAGGATCTGG ATGGGAAGAC TTCTGAGGGC CATCAGACCA CATGTGGCAC CATTTATAAT 
CCAACCCTTA GCATCAAGAA GCTGAGCCCA ATTATTGAAG ACAGTGGAGA AGCCACACAC 
TCATCTGGCT TCTCTGGTTC TGCTTCTGTT ACAAGTTCCT CCTCCATCAA AAGTCTTCAA 
ATTCCTGAGA AACTGGAGTT GACTGGAGAA GCTACAGAAA ATGCTATTCT GTCACCCTGG 
TGTTCACAAT ATCGGATACA GCTATTAAAA ACTCTACCAG AGTTAAGTGC TTCTGCAGAG 
TTTTTTATAG AAGACAGACC AATGCCTAAC TTGGAAATAG AGAAGGAAAT TGAATTAAGT 
AATGAAGATT ATTGCATTAA ACAAGAATAC CTAATATGTG AAGATTACAG GTTATTCTGG 
GTGGCACCAA GAAACTCCAC AGAATTGACC TTAATAAAGG TATCTTCTCA GCCTGTCCCA 
TGGGACTTTT ATATCAATCT TACATTAAAG GAACGTCTGA ATGAGGACTG TGATCAGTTT 
TGCAGCTATT ATCAATACCA AGATGGCTAT ATTGTTTGGT ACCAATATAT TAATTGCTCT 
ACCCTTCAGG ATCTTCTGCA ACACAGTGAA GTTATTACTC ATGAAATAGT AGTGTTGATT 
ATTTATAACC TTTTGACAAT AGTGGAGAAG CTACACAAAG CTGAAATCGT TCATGGAGAC 
TTGAGTCCAA ATAATCTGAT TCTTAGAAAT AGAATCCACG ATCTCTATGA TTGTAATAAG 
AACAGTCAAG CTTTGAAGAT AGTGGACTTT TCCTACAGCG TTGACCTGAG AGTGCAGCTA 
GATGTTTTTA CCCTCAGTGG CTTTCGGACT GTACAGACGC TGGAAGGACA AAAGATCCTG 
GCTAACTGTT CTTCTCCCTA CCAGGTAGAC CTGTTTGGTA TAGCAGATTT AGCACATTTA 
CTATTGTTCA AGGAACACCT ACAGGTCTTC TGGGATGGGT TCCTCTGGAA ACTTAGCCAA 
AATACTTCAG AGCTAAACGA TGGTGAATTG TGGAATAAAT TCTTTGTGCG GATTCTGAAT 
GCCAGTGATG AGTCCACAGT GTCTGTTCTG AGAGAACTTG CAGCAGAAAT GAATGGGATT 
TTTGACACCA CATTCCAAAG TCACCTGAAC AAAGCCTTAT GGAAAGTGGG GAAGGTAATT 
AGTCCAGGAG CTTTGCTCTT TCAGCAAGGT AGGCAGTCAA GGCTCTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008840535.1
CDS	222..3392
Translation	MAAAKKEGLAVSEAMSLEGDEWELSKENVQPLRHGRIMSTLQGALAQQESACNTTLQQQKRAFESEIRFYAGNDPLDVWDRYIKWTEQNYPQGGKESNMSTLLERAIEALQGEKRYYSDPRFLSLWLKLGHLCNEPLDMYSYLHSQGIGVSLAQFYISWAEEYEARENFKKADTVFQEGIQRKAEPLERLQSQHRQFQARVSRQALLALEKEEEEEVLGSSIPQRSTLAELKSKGKKTAKAPISRVGGALKAPGQSRGFQNIVPQQMQSNRRITVFDENSDITSRAELSKPEVQPWMAPPVPRAKENELQPGPWNTGRPLEYRPHGSTASLTAVPPMLPSFTPYVEETAQQPVMTPCKIEPSINHILSTRKPGKEEGDPFQRVQSHQQESEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKERREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEEKIPAKTDKLQIASESQEMTGVSLSHSVCPLNSHPRKTLPIENVLQEQPDSKGSSIPFSIFDEFLVSEKKNESPTDPPRALAQRQPLAVLKTTEIIASNEDMSPDVCDELTEIEPLSEDAIITGFRNVTICPNPEDTCDFARAAHFASTPFHEIMSLKDISSDPERLLQEEDLDGKTSEGHQTTCGTIYNPTLSIKKLSPIIEDSGEATHSSGFSGSASVTSSSSIKSLQIPEKLELTGEATENAILSPWCSQYRIQLLKTLPELSASAEFFIEDRPMPNLEIEKEIELSNEDYCIKQEYLICEDYRLFWVAPRNSTELTLIKVSSQPVPWDFYINLTLKERLNEDCDQFCSYYQYQDGYIVWYQYINCSTLQDLLQHSEVITHEIVVLIIYNLLTIVEKLHKAEIVHGDLSPNNLILRNRIHDLYDCNKNSQALKIVDFSYSVDLRVQLDVFTLSGFRTVQTLEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGFLWKLSQNTSELNDGELWNKFFVRILNASDESTVSVLRELAAEMNGIFDTTFQSHLNKALWKVGKVISPGALLFQQGRQSRLS

Target ORF information:

RefSeq Version	XM_008842313.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008842313.3

ORF Insert Sequence:

ATGGCGGCGG CCAAGAAGGA AGGGCTCGCC GTGAGTGAAG CTATGTCCCT GGAGGGAGAT 
GAGTGGGAGC TGAGTAAAGA AAATGTACAA CCCTTAAGGC ATGGGCGGAT CATGTCTACA 
CTTCAGGGAG CTTTGGCACA GCAGGAATCG GCCTGTAACA CTACTCTTCA GCAACAGAAA 
CGGGCATTTG AATCTGAAAT TCGCTTTTAC GCTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGGTATATTA AGTGGACAGA ACAGAACTAT CCTCAAGGGG GCAAGGAGAG CAACATGTCA 
ACATTACTCG AAAGAGCTAT AGAAGCTCTA CAAGGAGAAA AACGTTATTA TAGTGACCCT 
CGGTTTCTCA GTCTCTGGCT CAAATTGGGG CATTTGTGCA ATGAGCCATT GGACATGTAC 
AGTTATTTAC ACAGCCAAGG GATTGGCGTT TCACTTGCTC AGTTCTATAT TTCGTGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCTGATA CGGTATTTCA GGAAGGGATT 
CAACGGAAGG CCGAGCCACT AGAAAGACTG CAGTCCCAGC ACCGACAATT CCAGGCTCGG 
GTGTCTCGAC AAGCTCTCTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TTTAGGATCT 
TCCATTCCAC AGCGAAGCAC ATTAGCTGAG CTAAAGAGCA AAGGGAAGAA GACAGCAAAA 
GCTCCCATTA GCCGTGTAGG AGGTGCTCTG AAGGCTCCAG GTCAGAGCAG AGGATTCCAA 
AATATAGTTC CTCAGCAGAT GCAAAGTAAT CGCAGAATTA CTGTTTTTGA TGAAAATTCT 
GATATCACTT CTAGAGCAGA GTTATCTAAG CCCGAAGTCC AGCCCTGGAT GGCACCCCCT 
GTTCCAAGGG CCAAAGAGAA TGAGCTGCAG CCAGGCCCTT GGAACACAGG CAGGCCTTTG 
GAATACAGGC CTCATGGCAG TACAGCTTCT TTAACAGCTG TACCCCCTAT GCTTCCCAGC 
TTTACTCCAT ATGTGGAAGA GACTGCACAA CAACCAGTTA TGACACCATG TAAAATTGAA 
CCTAGTATAA ACCATATTCT AAGCACCAGA AAGCCTGGAA AGGAAGAAGG AGATCCTTTT 
CAAAGGGTTC AGAGTCATCA GCAAGAGTCT GAAGAAAAGA AAGAGAAGAT GATGTACTGT 
AAGGAGAAGA TTTATGCAGG AGTTGGGGAG TTCTCTTTTG AAGAAATCCG GGCTGAAGTT 
TTCCGGAAGA AATTAAAAGA GCGAAGGGAA GCGGAACTAC TGACCAGTGC AGAGAAGAGA 
GCAGAAATGC AGAAACAGAT TGAAGAGATG GAGAAAAAGC TCAAAGAAAT CCAGACTACT 
CAGCAGGAAA GAACTGGTGA TCAGCAAGAA GAGAAGATAC CTGCAAAGAC AGACAAACTG 
CAAATTGCTT CAGAGTCTCA GGAAATGACA GGAGTGTCTC TTTCCCATTC TGTTTGTCCA 
CTAAACTCTC ACCCCAGGAA AACTTTACCT ATAGAAAATG TTTTGCAAGA ACAGCCTGAT 
AGTAAAGGTT CCAGTATACC TTTCTCCATT TTTGATGAGT TTCTTGTTTC AGAAAAAAAG 
AATGAAAGTC CTACAGATCC TCCACGAGCT TTAGCACAGC GACAGCCTCT TGCAGTTTTA 
AAAACCACAG AAATAATTGC TTCAAATGAG GATATGTCTC CAGATGTTTG TGATGAACTT 
ACAGAAATTG AACCTTTGAG TGAGGATGCC ATTATCACAG GTTTCAGGAA TGTAACTATT 
TGTCCTAACC CAGAAGACAC ATGTGACTTC GCTAGAGCTG CTCATTTTGC CTCTACGCCT 
TTTCATGAGA TAATGTCCTT GAAGGATATC TCTTCCGATC CTGAGAGACT GCTGCAGGAA 
GAGGATCTGG ATGGGAAGAC TTCTGAGGGC CATCAGACCA CATGTGGCAC CATTTATAAT 
CCAACCCTTA GCATCAAGAA GCTGAGCCCA ATTATTGAAG ACAGTGGAGA AGCCACACAC 
TCATCTGGCT TCTCTGGTTC TGCTTCTGTT ACAAGTTCCT CCTCCATCAA AAGTCTTCAA 
ATTCCTGAGA AACTGGAGTT GACTGGAGAA GCTACAGAAA ATGCTATTCT GTCACCCTGG 
TGTTCACAAT ATCGGATACA GCTATTAAAA ACTCTACCAG AGTTAAGTGC TTCTGCAGAG 
TTTTTTATAG AAGACAGACC AATGCCTAAC TTGGAAATAG AGAAGGAAAT TGAATTAAGT 
AATGAAGATT ATTGCATTAA ACAAGAATAC CTAATATGTG AAGATTACAG GTTATTCTGG 
GTGGCACCAA GAAACTCCAC AGAATTGACC TTAATAAAGG TATCTTCTCA GCCTGTCCCA 
TGGGACTTTT ATATCAATCT TACATTAAAG GAACGTCTGA ATGAGGACTG TGATCAGTTT 
TGCAGCTATT ATCAATACCA AGATGGCTAT ATTGTTTGGT ACCAATATAT TAATTGCTCT 
ACCCTTCAGG ATCTTCTGCA ACACAGTGAA GTTATTACTC ATGAAATAGT AGTGTTGATT 
ATTTATAACC TTTTGACAAT AGTGGAGAAG CTACACAAAG CTGAAATCGT TCATGGAGAC 
TTGAGTCCAA ATAATCTGAT TCTTAGAAAT AGAATCCACG ATCTCTATGA TTGTAATAAG 
AACAGTCAAG CTTTGAAGAT AGTGGACTTT TCCTACAGCG TTGACCTGAG AGTGCAGCTA 
GATGTTTTTA CCCTCAGTGG CTTTCGGACT GTACAGACGC TGGAAGGACA AAAGATCCTG 
GCTAACTGTT CTTCTCCCTA CCAGGTAGAC CTGTTTGGTA TAGCAGATTT AGCACATTTA 
CTATTGTTCA AGGAACACCT ACAGGTCTTC TGGGATGGGT TCCTCTGGAA ACTTAGCCAA 
AATACTTCAG AGCTAAACGA TGGTGAATTG TGGAATAAAT TCTTTGTGCG GATTCTGAAT 
GCCAGTGATG AGTCCACAGT GTCTGTTCTG AGAGAACTTG CAGCAGAAAT GAATGGGATT 
TTTGACACCA CATTCCAAAG TCACCTGAAC AAAGCCTTAT GGAAAGTGGG GAAGGTAATT 
AGTCCAGGAG CTTTGCTCTT TCAGCAAGGT AGGCAGTCAA GGCTCTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bub1b ( XM_008842313.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008840535.1 Nannospalax galili BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), mRNA.

Price & Availability↑

CloneID	ONa24872
Clone ID Related Accession (Same CDS sequence)	XM_008842313.3 , XM_008842313.2
Accession Version	XM_008842313.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008345995.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008842313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCAAGAAGGA AGGGCTCGCC GTGAGTGAAG CTATGTCCCT GGAGGGAGAT 
GAGTGGGAGC TGAGTAAAGA AAATGTACAA CCCTTAAGGC ATGGGCGGAT CATGTCTACA 
CTTCAGGGAG CTTTGGCACA GCAGGAATCG GCCTGTAACA CTACTCTTCA GCAACAGAAA 
CGGGCATTTG AATCTGAAAT TCGCTTTTAC GCTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGGTATATTA AGTGGACAGA ACAGAACTAT CCTCAAGGGG GCAAGGAGAG CAACATGTCA 
ACATTACTCG AAAGAGCTAT AGAAGCTCTA CAAGGAGAAA AACGTTATTA TAGTGACCCT 
CGGTTTCTCA GTCTCTGGCT CAAATTGGGG CATTTGTGCA ATGAGCCATT GGACATGTAC 
AGTTATTTAC ACAGCCAAGG GATTGGCGTT TCACTTGCTC AGTTCTATAT TTCGTGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCTGATA CGGTATTTCA GGAAGGGATT 
CAACGGAAGG CCGAGCCACT AGAAAGACTG CAGTCCCAGC ACCGACAATT CCAGGCTCGG 
GTGTCTCGAC AAGCTCTCTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TTTAGGATCT 
TCCATTCCAC AGCGAAGCAC ATTAGCTGAG CTAAAGAGCA AAGGGAAGAA GACAGCAAAA 
GCTCCCATTA GCCGTGTAGG AGGTGCTCTG AAGGCTCCAG GTCAGAGCAG AGGATTCCAA 
AATATAGTTC CTCAGCAGAT GCAAAGTAAT CGCAGAATTA CTGTTTTTGA TGAAAATTCT 
GATATCACTT CTAGAGCAGA GTTATCTAAG CCCGAAGTCC AGCCCTGGAT GGCACCCCCT 
GTTCCAAGGG CCAAAGAGAA TGAGCTGCAG CCAGGCCCTT GGAACACAGG CAGGCCTTTG 
GAATACAGGC CTCATGGCAG TACAGCTTCT TTAACAGCTG TACCCCCTAT GCTTCCCAGC 
TTTACTCCAT ATGTGGAAGA GACTGCACAA CAACCAGTTA TGACACCATG TAAAATTGAA 
CCTAGTATAA ACCATATTCT AAGCACCAGA AAGCCTGGAA AGGAAGAAGG AGATCCTTTT 
CAAAGGGTTC AGAGTCATCA GCAAGAGTCT GAAGAAAAGA AAGAGAAGAT GATGTACTGT 
AAGGAGAAGA TTTATGCAGG AGTTGGGGAG TTCTCTTTTG AAGAAATCCG GGCTGAAGTT 
TTCCGGAAGA AATTAAAAGA GCGAAGGGAA GCGGAACTAC TGACCAGTGC AGAGAAGAGA 
GCAGAAATGC AGAAACAGAT TGAAGAGATG GAGAAAAAGC TCAAAGAAAT CCAGACTACT 
CAGCAGGAAA GAACTGGTGA TCAGCAAGAA GAGAAGATAC CTGCAAAGAC AGACAAACTG 
CAAATTGCTT CAGAGTCTCA GGAAATGACA GGAGTGTCTC TTTCCCATTC TGTTTGTCCA 
CTAAACTCTC ACCCCAGGAA AACTTTACCT ATAGAAAATG TTTTGCAAGA ACAGCCTGAT 
AGTAAAGGTT CCAGTATACC TTTCTCCATT TTTGATGAGT TTCTTGTTTC AGAAAAAAAG 
AATGAAAGTC CTACAGATCC TCCACGAGCT TTAGCACAGC GACAGCCTCT TGCAGTTTTA 
AAAACCACAG AAATAATTGC TTCAAATGAG GATATGTCTC CAGATGTTTG TGATGAACTT 
ACAGAAATTG AACCTTTGAG TGAGGATGCC ATTATCACAG GTTTCAGGAA TGTAACTATT 
TGTCCTAACC CAGAAGACAC ATGTGACTTC GCTAGAGCTG CTCATTTTGC CTCTACGCCT 
TTTCATGAGA TAATGTCCTT GAAGGATATC TCTTCCGATC CTGAGAGACT GCTGCAGGAA 
GAGGATCTGG ATGGGAAGAC TTCTGAGGGC CATCAGACCA CATGTGGCAC CATTTATAAT 
CCAACCCTTA GCATCAAGAA GCTGAGCCCA ATTATTGAAG ACAGTGGAGA AGCCACACAC 
TCATCTGGCT TCTCTGGTTC TGCTTCTGTT ACAAGTTCCT CCTCCATCAA AAGTCTTCAA 
ATTCCTGAGA AACTGGAGTT GACTGGAGAA GCTACAGAAA ATGCTATTCT GTCACCCTGG 
TGTTCACAAT ATCGGATACA GCTATTAAAA ACTCTACCAG AGTTAAGTGC TTCTGCAGAG 
TTTTTTATAG AAGACAGACC AATGCCTAAC TTGGAAATAG AGAAGGAAAT TGAATTAAGT 
AATGAAGATT ATTGCATTAA ACAAGAATAC CTAATATGTG AAGATTACAG GTTATTCTGG 
GTGGCACCAA GAAACTCCAC AGAATTGACC TTAATAAAGG TATCTTCTCA GCCTGTCCCA 
TGGGACTTTT ATATCAATCT TACATTAAAG GAACGTCTGA ATGAGGACTG TGATCAGTTT 
TGCAGCTATT ATCAATACCA AGATGGCTAT ATTGTTTGGT ACCAATATAT TAATTGCTCT 
ACCCTTCAGG ATCTTCTGCA ACACAGTGAA GTTATTACTC ATGAAATAGT AGTGTTGATT 
ATTTATAACC TTTTGACAAT AGTGGAGAAG CTACACAAAG CTGAAATCGT TCATGGAGAC 
TTGAGTCCAA ATAATCTGAT TCTTAGAAAT AGAATCCACG ATCTCTATGA TTGTAATAAG 
AACAGTCAAG CTTTGAAGAT AGTGGACTTT TCCTACAGCG TTGACCTGAG AGTGCAGCTA 
GATGTTTTTA CCCTCAGTGG CTTTCGGACT GTACAGACGC TGGAAGGACA AAAGATCCTG 
GCTAACTGTT CTTCTCCCTA CCAGGTAGAC CTGTTTGGTA TAGCAGATTT AGCACATTTA 
CTATTGTTCA AGGAACACCT ACAGGTCTTC TGGGATGGGT TCCTCTGGAA ACTTAGCCAA 
AATACTTCAG AGCTAAACGA TGGTGAATTG TGGAATAAAT TCTTTGTGCG GATTCTGAAT 
GCCAGTGATG AGTCCACAGT GTCTGTTCTG AGAGAACTTG CAGCAGAAAT GAATGGGATT 
TTTGACACCA CATTCCAAAG TCACCTGAAC AAAGCCTTAT GGAAAGTGGG GAAGGTAATT 
AGTCCAGGAG CTTTGCTCTT TCAGCAAGGT AGGCAGTCAA GGCTCTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008840535.1
CDS	29..3199
Translation	MAAAKKEGLAVSEAMSLEGDEWELSKENVQPLRHGRIMSTLQGALAQQESACNTTLQQQKRAFESEIRFYAGNDPLDVWDRYIKWTEQNYPQGGKESNMSTLLERAIEALQGEKRYYSDPRFLSLWLKLGHLCNEPLDMYSYLHSQGIGVSLAQFYISWAEEYEARENFKKADTVFQEGIQRKAEPLERLQSQHRQFQARVSRQALLALEKEEEEEVLGSSIPQRSTLAELKSKGKKTAKAPISRVGGALKAPGQSRGFQNIVPQQMQSNRRITVFDENSDITSRAELSKPEVQPWMAPPVPRAKENELQPGPWNTGRPLEYRPHGSTASLTAVPPMLPSFTPYVEETAQQPVMTPCKIEPSINHILSTRKPGKEEGDPFQRVQSHQQESEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKERREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEEKIPAKTDKLQIASESQEMTGVSLSHSVCPLNSHPRKTLPIENVLQEQPDSKGSSIPFSIFDEFLVSEKKNESPTDPPRALAQRQPLAVLKTTEIIASNEDMSPDVCDELTEIEPLSEDAIITGFRNVTICPNPEDTCDFARAAHFASTPFHEIMSLKDISSDPERLLQEEDLDGKTSEGHQTTCGTIYNPTLSIKKLSPIIEDSGEATHSSGFSGSASVTSSSSIKSLQIPEKLELTGEATENAILSPWCSQYRIQLLKTLPELSASAEFFIEDRPMPNLEIEKEIELSNEDYCIKQEYLICEDYRLFWVAPRNSTELTLIKVSSQPVPWDFYINLTLKERLNEDCDQFCSYYQYQDGYIVWYQYINCSTLQDLLQHSEVITHEIVVLIIYNLLTIVEKLHKAEIVHGDLSPNNLILRNRIHDLYDCNKNSQALKIVDFSYSVDLRVQLDVFTLSGFRTVQTLEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGFLWKLSQNTSELNDGELWNKFFVRILNASDESTVSVLRELAAEMNGIFDTTFQSHLNKALWKVGKVISPGALLFQQGRQSRLS

Target ORF information:

RefSeq Version	XM_008842313.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008842313.2

ORF Insert Sequence:

ATGGCGGCGG CCAAGAAGGA AGGGCTCGCC GTGAGTGAAG CTATGTCCCT GGAGGGAGAT 
GAGTGGGAGC TGAGTAAAGA AAATGTACAA CCCTTAAGGC ATGGGCGGAT CATGTCTACA 
CTTCAGGGAG CTTTGGCACA GCAGGAATCG GCCTGTAACA CTACTCTTCA GCAACAGAAA 
CGGGCATTTG AATCTGAAAT TCGCTTTTAC GCTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGGTATATTA AGTGGACAGA ACAGAACTAT CCTCAAGGGG GCAAGGAGAG CAACATGTCA 
ACATTACTCG AAAGAGCTAT AGAAGCTCTA CAAGGAGAAA AACGTTATTA TAGTGACCCT 
CGGTTTCTCA GTCTCTGGCT CAAATTGGGG CATTTGTGCA ATGAGCCATT GGACATGTAC 
AGTTATTTAC ACAGCCAAGG GATTGGCGTT TCACTTGCTC AGTTCTATAT TTCGTGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCTGATA CGGTATTTCA GGAAGGGATT 
CAACGGAAGG CCGAGCCACT AGAAAGACTG CAGTCCCAGC ACCGACAATT CCAGGCTCGG 
GTGTCTCGAC AAGCTCTCTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TTTAGGATCT 
TCCATTCCAC AGCGAAGCAC ATTAGCTGAG CTAAAGAGCA AAGGGAAGAA GACAGCAAAA 
GCTCCCATTA GCCGTGTAGG AGGTGCTCTG AAGGCTCCAG GTCAGAGCAG AGGATTCCAA 
AATATAGTTC CTCAGCAGAT GCAAAGTAAT CGCAGAATTA CTGTTTTTGA TGAAAATTCT 
GATATCACTT CTAGAGCAGA GTTATCTAAG CCCGAAGTCC AGCCCTGGAT GGCACCCCCT 
GTTCCAAGGG CCAAAGAGAA TGAGCTGCAG CCAGGCCCTT GGAACACAGG CAGGCCTTTG 
GAATACAGGC CTCATGGCAG TACAGCTTCT TTAACAGCTG TACCCCCTAT GCTTCCCAGC 
TTTACTCCAT ATGTGGAAGA GACTGCACAA CAACCAGTTA TGACACCATG TAAAATTGAA 
CCTAGTATAA ACCATATTCT AAGCACCAGA AAGCCTGGAA AGGAAGAAGG AGATCCTTTT 
CAAAGGGTTC AGAGTCATCA GCAAGAGTCT GAAGAAAAGA AAGAGAAGAT GATGTACTGT 
AAGGAGAAGA TTTATGCAGG AGTTGGGGAG TTCTCTTTTG AAGAAATCCG GGCTGAAGTT 
TTCCGGAAGA AATTAAAAGA GCGAAGGGAA GCGGAACTAC TGACCAGTGC AGAGAAGAGA 
GCAGAAATGC AGAAACAGAT TGAAGAGATG GAGAAAAAGC TCAAAGAAAT CCAGACTACT 
CAGCAGGAAA GAACTGGTGA TCAGCAAGAA GAGAAGATAC CTGCAAAGAC AGACAAACTG 
CAAATTGCTT CAGAGTCTCA GGAAATGACA GGAGTGTCTC TTTCCCATTC TGTTTGTCCA 
CTAAACTCTC ACCCCAGGAA AACTTTACCT ATAGAAAATG TTTTGCAAGA ACAGCCTGAT 
AGTAAAGGTT CCAGTATACC TTTCTCCATT TTTGATGAGT TTCTTGTTTC AGAAAAAAAG 
AATGAAAGTC CTACAGATCC TCCACGAGCT TTAGCACAGC GACAGCCTCT TGCAGTTTTA 
AAAACCACAG AAATAATTGC TTCAAATGAG GATATGTCTC CAGATGTTTG TGATGAACTT 
ACAGAAATTG AACCTTTGAG TGAGGATGCC ATTATCACAG GTTTCAGGAA TGTAACTATT 
TGTCCTAACC CAGAAGACAC ATGTGACTTC GCTAGAGCTG CTCATTTTGC CTCTACGCCT 
TTTCATGAGA TAATGTCCTT GAAGGATATC TCTTCCGATC CTGAGAGACT GCTGCAGGAA 
GAGGATCTGG ATGGGAAGAC TTCTGAGGGC CATCAGACCA CATGTGGCAC CATTTATAAT 
CCAACCCTTA GCATCAAGAA GCTGAGCCCA ATTATTGAAG ACAGTGGAGA AGCCACACAC 
TCATCTGGCT TCTCTGGTTC TGCTTCTGTT ACAAGTTCCT CCTCCATCAA AAGTCTTCAA 
ATTCCTGAGA AACTGGAGTT GACTGGAGAA GCTACAGAAA ATGCTATTCT GTCACCCTGG 
TGTTCACAAT ATCGGATACA GCTATTAAAA ACTCTACCAG AGTTAAGTGC TTCTGCAGAG 
TTTTTTATAG AAGACAGACC AATGCCTAAC TTGGAAATAG AGAAGGAAAT TGAATTAAGT 
AATGAAGATT ATTGCATTAA ACAAGAATAC CTAATATGTG AAGATTACAG GTTATTCTGG 
GTGGCACCAA GAAACTCCAC AGAATTGACC TTAATAAAGG TATCTTCTCA GCCTGTCCCA 
TGGGACTTTT ATATCAATCT TACATTAAAG GAACGTCTGA ATGAGGACTG TGATCAGTTT 
TGCAGCTATT ATCAATACCA AGATGGCTAT ATTGTTTGGT ACCAATATAT TAATTGCTCT 
ACCCTTCAGG ATCTTCTGCA ACACAGTGAA GTTATTACTC ATGAAATAGT AGTGTTGATT 
ATTTATAACC TTTTGACAAT AGTGGAGAAG CTACACAAAG CTGAAATCGT TCATGGAGAC 
TTGAGTCCAA ATAATCTGAT TCTTAGAAAT AGAATCCACG ATCTCTATGA TTGTAATAAG 
AACAGTCAAG CTTTGAAGAT AGTGGACTTT TCCTACAGCG TTGACCTGAG AGTGCAGCTA 
GATGTTTTTA CCCTCAGTGG CTTTCGGACT GTACAGACGC TGGAAGGACA AAAGATCCTG 
GCTAACTGTT CTTCTCCCTA CCAGGTAGAC CTGTTTGGTA TAGCAGATTT AGCACATTTA 
CTATTGTTCA AGGAACACCT ACAGGTCTTC TGGGATGGGT TCCTCTGGAA ACTTAGCCAA 
AATACTTCAG AGCTAAACGA TGGTGAATTG TGGAATAAAT TCTTTGTGCG GATTCTGAAT 
GCCAGTGATG AGTCCACAGT GTCTGTTCTG AGAGAACTTG CAGCAGAAAT GAATGGGATT 
TTTGACACCA CATTCCAAAG TCACCTGAAC AAAGCCTTAT GGAAAGTGGG GAAGGTAATT 
AGTCCAGGAG CTTTGCTCTT TCAGCAAGGT AGGCAGTCAA GGCTCTCATA A