Map4k5 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map4k5
Entrez Gene ID	103731509
Full Name	mitogen-activated protein kinase kinase kinase kinase 5
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008829845.2	XP_008828067.1	mitogen-activated protein kinase kinase kinase kinase 5
XM_008829845.3	XP_008828067.1	mitogen-activated protein kinase kinase kinase kinase 5

Pathways from BioSystems
Gene Ontology

The following Map4k5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map4k5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa46522	XM_008829845.3 Latest version!	Nannospalax galili mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ONa46522	XM_008829845.2	Nannospalax galili mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map4k5 ( XM_008829845.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008828067.1 Nannospalax galili mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), mRNA.

Price & Availability↑

CloneID	ONa46522
Clone ID Related Accession (Same CDS sequence)	XM_008829845.2 , XM_008829845.3
Accession Version	XM_008829845.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	mitogen-activated protein kinase kinase kinase kinase 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008335287.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008829845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC CGCTGCGGCC TGCCGCCGAC ATCCTGAGGC GGAACCCGCA GCACGACTAC 
GAGCTGGTCC AAAGGGTCGG CAGCGGTACC TACGGGGACG TCTACAAGGC CAGAAATGTA 
CATACAGGAG AATTAGCTGC AGTAAAAATC ATCAAATTGG AGCCTGGTGA TGATTTTTCT 
TTGATCCAAC AAGAAATATT TATGGTTAAA GAATGTAAAC ATTGCAACAT CGTAGCCTAC 
TTTGGGAGCT ATCTCAGTAG GGAAAAACTA TGGATTTGTA TGGAATACTG TGGTGGTGGA 
TCACTTCAAG ATATTTATCA TGTTACTGGA CCATTATCTG AATTGCAAAT AGCCTATGTG 
TGCAGAGAGA CCTTACAGGG TCTTGCCTAT TTGCATACTA AAGGCAAAAT GCATAGAGAT 
ATCAAAGGTG CAAATATCTT ATTAACAGAC CATGGTGATG TAAAATTGGC TGATTTTGGT 
GTGGCTGCAA AAATAACAGC TACCATTGCA AAGAGAAAGT CTTTCATCGG CACCCCTTAT 
TGGATGGCCC CAGAAGTTGC AGCAGTGGAG AAAAATGGTG GCTACAACCA ACTTTGTGAT 
ATCTGGGCAG TAGGAATAAC AGCAATTGAA CTTGGAGAGC TTCAGCCACC TATGTTTGAT 
CTTCATCCAA TGAGGGCTCT CTTCTTAATG TCAAAAAGTA ATTTTCAGCC TCCGAAACTA 
AAGGATAAAA CAAAATGGTC ATCAACATTC CATAATTTTG TCAAAATAGC ACTAACTAAA 
AACCCTAAAA AAAGACCAAC TGCTGAAAGA CTTCTAACGC ATACTTTCGT TGGGCAGTCA 
GGTCTGTCTC GGGCCCTAGC AGTTGAGCTG TTGGACAAAG TGAGCAATCC GGACAACCAT 
GCACATTACA CTGAAGGAGA TGACGATGAC TTTGAGCCCC ATGCAATCAT TCGTCATACC 
ATTAGATCTA CAAACAGGAA TGCCAGAGCT GAACGGACAG CTTCAGAAAT AAATTTTGAC 
AAATTACAGT TTGAACCTCC CCTGAGAAAA GAAACAGAAG CACGGGATGA AATGGGAATA 
TCATCCGACC CAAACTTTAT ATTACATTGG AATCCTTTTG TGGATGGTGC AAATACGGGC 
AAATCAACCT CAAAACGTGC AATACCGCCT CCCCTACCTC CTAAGCCAAG GATAAACAGT 
TTCCCTGAAG ACAACTTCCC AGATGATGAA AAATCATCAA CTGTCAAACG TTGCCCTGAT 
TTAGAGAACA GAGCTCCTCA AGTTCTCAGA AGACAAAGCA GCCCAAGTTG TGGTCCTGTG 
GCAGAGAGCT CCTTTATGGG AAATGGTGAT GGTATTTCAA AACTGATCAG TGAGAATACA 
GAGGGATCAG CACAAGCTCC GCAGTTACCA CGGAAAAAGG ACAAGCGAGA ATTCCCTAAA 
CCAGCTATCA ATGGCCTTCC ACCCACCCCA AAAGTACTGA TGGGAGCATG TTTTTCAAAA 
GTTTTTGATG GCTGCCCTTT GAAAATTAAT TGTGCAACAT CCTGGATACA TCCTGATACA 
AAAGATCAGT ATATCATTTT TGGAACTGAA GATGGTATTT ACACATTGAA TCTCAATGAG 
CTACACGAGG CAACTATGGA ACAGTTATTT CCACGGAAAT GTACATGGCT CTATGTTATC 
AATAATACTT TAATGTCATT ATCAGAAGGA AAATCCTTTC AGCTCTACTC TCATAATCTT 
ATTGCATTGT TTGAACAAGC CAAAAAACCA GGATTAGCTG CCCATATCCA GACTCATAGA 
TTTCCAGATC GCATTCTGCC AAGAAAGTTT GCCTTAACAA CAAAGATTCC TGATACAAAA 
GGCTGCCATA AATGTTGCAT AGTCAGAAAC CCTTATACAG GACATAAGTA CCTATGTGGA 
GCTTTGCAGT CTGGCATTGT TCTGCTTCAA TGGTATGAAC CAATGCAGAA ATTCATGCTG 
ATAAAGCACT TTGACTTTCC TTTACCAAGT CCTTTGAATG TTTTTGAGAT GCTGGTGATA 
CCAGAACAGG AATACCCGAT GGTCTGTGTA GCTATCAGTA AAGGCACAGA ATCAAATCAA 
GTAGTTCAGT TTGAAACAAT CAATTTGAAT TCTGCATCTT CATGGTTTAC AGAAATCGGT 
GCAGGCAGCC AACAGCTGGA TTCTATTCAT GTAACACAGC TGGAGAGAGA TACCGTTTTG 
GTGTGTTTAG ATAAATTTGT GAAAATTGTA AATCTACAAG GAAAACTAAA ATCAAGTAAG 
AAACTGGCCT CTGAGCTAAG TTTTGACTTT CGCATTGAAT CTGTAGTATG CCTTCAAGAC 
AGTGTGTTGG CTTTCTGGAA ACATGGGATG CAGGGTAAAA GTTTCAAATC GGATGAGGTA 
ACCCAGGAAA TTTCAGATGA AACCAGAGTT TTCCGCTTAT TGGGATCAGA CAGGGTTGTT 
GTTTTGGAAA GCAGACCAAC AGAAAACCCT ACAGCACATA GCAATCTCTA CATCTTGGCT 
GGACACGAAA ATAGTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008828067.1
CDS	161..2701
Translation	MEAPLRPAADILRRNPQHDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFSLIQQEIFMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSELQIAYVCRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTFVGQSGLSRALAVELLDKVSNPDNHAHYTEGDDDDFEPHAIIRHTIRSTNRNARAERTASEINFDKLQFEPPLRKETEARDEMGISSDPNFILHWNPFVDGANTGKSTSKRAIPPPLPPKPRINSFPEDNFPDDEKSSTVKRCPDLENRAPQVLRRQSSPSCGPVAESSFMGNGDGISKLISENTEGSAQAPQLPRKKDKREFPKPAINGLPPTPKVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEDGIYTLNLNELHEATMEQLFPRKCTWLYVINNTLMSLSEGKSFQLYSHNLIALFEQAKKPGLAAHIQTHRFPDRILPRKFALTTKIPDTKGCHKCCIVRNPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGTESNQVVQFETINLNSASSWFTEIGAGSQQLDSIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFDFRIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPTAHSNLYILAGHENSY

Target ORF information:

RefSeq Version	XM_008829845.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008829845.2

ORF Insert Sequence:

ATGGAGGCCC CGCTGCGGCC TGCCGCCGAC ATCCTGAGGC GGAACCCGCA GCACGACTAC 
GAGCTGGTCC AAAGGGTCGG CAGCGGTACC TACGGGGACG TCTACAAGGC CAGAAATGTA 
CATACAGGAG AATTAGCTGC AGTAAAAATC ATCAAATTGG AGCCTGGTGA TGATTTTTCT 
TTGATCCAAC AAGAAATATT TATGGTTAAA GAATGTAAAC ATTGCAACAT CGTAGCCTAC 
TTTGGGAGCT ATCTCAGTAG GGAAAAACTA TGGATTTGTA TGGAATACTG TGGTGGTGGA 
TCACTTCAAG ATATTTATCA TGTTACTGGA CCATTATCTG AATTGCAAAT AGCCTATGTG 
TGCAGAGAGA CCTTACAGGG TCTTGCCTAT TTGCATACTA AAGGCAAAAT GCATAGAGAT 
ATCAAAGGTG CAAATATCTT ATTAACAGAC CATGGTGATG TAAAATTGGC TGATTTTGGT 
GTGGCTGCAA AAATAACAGC TACCATTGCA AAGAGAAAGT CTTTCATCGG CACCCCTTAT 
TGGATGGCCC CAGAAGTTGC AGCAGTGGAG AAAAATGGTG GCTACAACCA ACTTTGTGAT 
ATCTGGGCAG TAGGAATAAC AGCAATTGAA CTTGGAGAGC TTCAGCCACC TATGTTTGAT 
CTTCATCCAA TGAGGGCTCT CTTCTTAATG TCAAAAAGTA ATTTTCAGCC TCCGAAACTA 
AAGGATAAAA CAAAATGGTC ATCAACATTC CATAATTTTG TCAAAATAGC ACTAACTAAA 
AACCCTAAAA AAAGACCAAC TGCTGAAAGA CTTCTAACGC ATACTTTCGT TGGGCAGTCA 
GGTCTGTCTC GGGCCCTAGC AGTTGAGCTG TTGGACAAAG TGAGCAATCC GGACAACCAT 
GCACATTACA CTGAAGGAGA TGACGATGAC TTTGAGCCCC ATGCAATCAT TCGTCATACC 
ATTAGATCTA CAAACAGGAA TGCCAGAGCT GAACGGACAG CTTCAGAAAT AAATTTTGAC 
AAATTACAGT TTGAACCTCC CCTGAGAAAA GAAACAGAAG CACGGGATGA AATGGGAATA 
TCATCCGACC CAAACTTTAT ATTACATTGG AATCCTTTTG TGGATGGTGC AAATACGGGC 
AAATCAACCT CAAAACGTGC AATACCGCCT CCCCTACCTC CTAAGCCAAG GATAAACAGT 
TTCCCTGAAG ACAACTTCCC AGATGATGAA AAATCATCAA CTGTCAAACG TTGCCCTGAT 
TTAGAGAACA GAGCTCCTCA AGTTCTCAGA AGACAAAGCA GCCCAAGTTG TGGTCCTGTG 
GCAGAGAGCT CCTTTATGGG AAATGGTGAT GGTATTTCAA AACTGATCAG TGAGAATACA 
GAGGGATCAG CACAAGCTCC GCAGTTACCA CGGAAAAAGG ACAAGCGAGA ATTCCCTAAA 
CCAGCTATCA ATGGCCTTCC ACCCACCCCA AAAGTACTGA TGGGAGCATG TTTTTCAAAA 
GTTTTTGATG GCTGCCCTTT GAAAATTAAT TGTGCAACAT CCTGGATACA TCCTGATACA 
AAAGATCAGT ATATCATTTT TGGAACTGAA GATGGTATTT ACACATTGAA TCTCAATGAG 
CTACACGAGG CAACTATGGA ACAGTTATTT CCACGGAAAT GTACATGGCT CTATGTTATC 
AATAATACTT TAATGTCATT ATCAGAAGGA AAATCCTTTC AGCTCTACTC TCATAATCTT 
ATTGCATTGT TTGAACAAGC CAAAAAACCA GGATTAGCTG CCCATATCCA GACTCATAGA 
TTTCCAGATC GCATTCTGCC AAGAAAGTTT GCCTTAACAA CAAAGATTCC TGATACAAAA 
GGCTGCCATA AATGTTGCAT AGTCAGAAAC CCTTATACAG GACATAAGTA CCTATGTGGA 
GCTTTGCAGT CTGGCATTGT TCTGCTTCAA TGGTATGAAC CAATGCAGAA ATTCATGCTG 
ATAAAGCACT TTGACTTTCC TTTACCAAGT CCTTTGAATG TTTTTGAGAT GCTGGTGATA 
CCAGAACAGG AATACCCGAT GGTCTGTGTA GCTATCAGTA AAGGCACAGA ATCAAATCAA 
GTAGTTCAGT TTGAAACAAT CAATTTGAAT TCTGCATCTT CATGGTTTAC AGAAATCGGT 
GCAGGCAGCC AACAGCTGGA TTCTATTCAT GTAACACAGC TGGAGAGAGA TACCGTTTTG 
GTGTGTTTAG ATAAATTTGT GAAAATTGTA AATCTACAAG GAAAACTAAA ATCAAGTAAG 
AAACTGGCCT CTGAGCTAAG TTTTGACTTT CGCATTGAAT CTGTAGTATG CCTTCAAGAC 
AGTGTGTTGG CTTTCTGGAA ACATGGGATG CAGGGTAAAA GTTTCAAATC GGATGAGGTA 
ACCCAGGAAA TTTCAGATGA AACCAGAGTT TTCCGCTTAT TGGGATCAGA CAGGGTTGTT 
GTTTTGGAAA GCAGACCAAC AGAAAACCCT ACAGCACATA GCAATCTCTA CATCTTGGCT 
GGACACGAAA ATAGTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k5 ( XM_008829845.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008828067.1 Nannospalax galili mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), mRNA.

Price & Availability↑

CloneID	ONa46522
Clone ID Related Accession (Same CDS sequence)	XM_008829845.2 , XM_008829845.3
Accession Version	XM_008829845.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	mitogen-activated protein kinase kinase kinase kinase 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008335287.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008829845.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC CGCTGCGGCC TGCCGCCGAC ATCCTGAGGC GGAACCCGCA GCACGACTAC 
GAGCTGGTCC AAAGGGTCGG CAGCGGTACC TACGGGGACG TCTACAAGGC CAGAAATGTA 
CATACAGGAG AATTAGCTGC AGTAAAAATC ATCAAATTGG AGCCTGGTGA TGATTTTTCT 
TTGATCCAAC AAGAAATATT TATGGTTAAA GAATGTAAAC ATTGCAACAT CGTAGCCTAC 
TTTGGGAGCT ATCTCAGTAG GGAAAAACTA TGGATTTGTA TGGAATACTG TGGTGGTGGA 
TCACTTCAAG ATATTTATCA TGTTACTGGA CCATTATCTG AATTGCAAAT AGCCTATGTG 
TGCAGAGAGA CCTTACAGGG TCTTGCCTAT TTGCATACTA AAGGCAAAAT GCATAGAGAT 
ATCAAAGGTG CAAATATCTT ATTAACAGAC CATGGTGATG TAAAATTGGC TGATTTTGGT 
GTGGCTGCAA AAATAACAGC TACCATTGCA AAGAGAAAGT CTTTCATCGG CACCCCTTAT 
TGGATGGCCC CAGAAGTTGC AGCAGTGGAG AAAAATGGTG GCTACAACCA ACTTTGTGAT 
ATCTGGGCAG TAGGAATAAC AGCAATTGAA CTTGGAGAGC TTCAGCCACC TATGTTTGAT 
CTTCATCCAA TGAGGGCTCT CTTCTTAATG TCAAAAAGTA ATTTTCAGCC TCCGAAACTA 
AAGGATAAAA CAAAATGGTC ATCAACATTC CATAATTTTG TCAAAATAGC ACTAACTAAA 
AACCCTAAAA AAAGACCAAC TGCTGAAAGA CTTCTAACGC ATACTTTCGT TGGGCAGTCA 
GGTCTGTCTC GGGCCCTAGC AGTTGAGCTG TTGGACAAAG TGAGCAATCC GGACAACCAT 
GCACATTACA CTGAAGGAGA TGACGATGAC TTTGAGCCCC ATGCAATCAT TCGTCATACC 
ATTAGATCTA CAAACAGGAA TGCCAGAGCT GAACGGACAG CTTCAGAAAT AAATTTTGAC 
AAATTACAGT TTGAACCTCC CCTGAGAAAA GAAACAGAAG CACGGGATGA AATGGGAATA 
TCATCCGACC CAAACTTTAT ATTACATTGG AATCCTTTTG TGGATGGTGC AAATACGGGC 
AAATCAACCT CAAAACGTGC AATACCGCCT CCCCTACCTC CTAAGCCAAG GATAAACAGT 
TTCCCTGAAG ACAACTTCCC AGATGATGAA AAATCATCAA CTGTCAAACG TTGCCCTGAT 
TTAGAGAACA GAGCTCCTCA AGTTCTCAGA AGACAAAGCA GCCCAAGTTG TGGTCCTGTG 
GCAGAGAGCT CCTTTATGGG AAATGGTGAT GGTATTTCAA AACTGATCAG TGAGAATACA 
GAGGGATCAG CACAAGCTCC GCAGTTACCA CGGAAAAAGG ACAAGCGAGA ATTCCCTAAA 
CCAGCTATCA ATGGCCTTCC ACCCACCCCA AAAGTACTGA TGGGAGCATG TTTTTCAAAA 
GTTTTTGATG GCTGCCCTTT GAAAATTAAT TGTGCAACAT CCTGGATACA TCCTGATACA 
AAAGATCAGT ATATCATTTT TGGAACTGAA GATGGTATTT ACACATTGAA TCTCAATGAG 
CTACACGAGG CAACTATGGA ACAGTTATTT CCACGGAAAT GTACATGGCT CTATGTTATC 
AATAATACTT TAATGTCATT ATCAGAAGGA AAATCCTTTC AGCTCTACTC TCATAATCTT 
ATTGCATTGT TTGAACAAGC CAAAAAACCA GGATTAGCTG CCCATATCCA GACTCATAGA 
TTTCCAGATC GCATTCTGCC AAGAAAGTTT GCCTTAACAA CAAAGATTCC TGATACAAAA 
GGCTGCCATA AATGTTGCAT AGTCAGAAAC CCTTATACAG GACATAAGTA CCTATGTGGA 
GCTTTGCAGT CTGGCATTGT TCTGCTTCAA TGGTATGAAC CAATGCAGAA ATTCATGCTG 
ATAAAGCACT TTGACTTTCC TTTACCAAGT CCTTTGAATG TTTTTGAGAT GCTGGTGATA 
CCAGAACAGG AATACCCGAT GGTCTGTGTA GCTATCAGTA AAGGCACAGA ATCAAATCAA 
GTAGTTCAGT TTGAAACAAT CAATTTGAAT TCTGCATCTT CATGGTTTAC AGAAATCGGT 
GCAGGCAGCC AACAGCTGGA TTCTATTCAT GTAACACAGC TGGAGAGAGA TACCGTTTTG 
GTGTGTTTAG ATAAATTTGT GAAAATTGTA AATCTACAAG GAAAACTAAA ATCAAGTAAG 
AAACTGGCCT CTGAGCTAAG TTTTGACTTT CGCATTGAAT CTGTAGTATG CCTTCAAGAC 
AGTGTGTTGG CTTTCTGGAA ACATGGGATG CAGGGTAAAA GTTTCAAATC GGATGAGGTA 
ACCCAGGAAA TTTCAGATGA AACCAGAGTT TTCCGCTTAT TGGGATCAGA CAGGGTTGTT 
GTTTTGGAAA GCAGACCAAC AGAAAACCCT ACAGCACATA GCAATCTCTA CATCTTGGCT 
GGACACGAAA ATAGTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008828067.1
CDS	242..2782
Translation	MEAPLRPAADILRRNPQHDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFSLIQQEIFMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSELQIAYVCRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTFVGQSGLSRALAVELLDKVSNPDNHAHYTEGDDDDFEPHAIIRHTIRSTNRNARAERTASEINFDKLQFEPPLRKETEARDEMGISSDPNFILHWNPFVDGANTGKSTSKRAIPPPLPPKPRINSFPEDNFPDDEKSSTVKRCPDLENRAPQVLRRQSSPSCGPVAESSFMGNGDGISKLISENTEGSAQAPQLPRKKDKREFPKPAINGLPPTPKVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEDGIYTLNLNELHEATMEQLFPRKCTWLYVINNTLMSLSEGKSFQLYSHNLIALFEQAKKPGLAAHIQTHRFPDRILPRKFALTTKIPDTKGCHKCCIVRNPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGTESNQVVQFETINLNSASSWFTEIGAGSQQLDSIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFDFRIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPTAHSNLYILAGHENSY

Target ORF information:

RefSeq Version	XM_008829845.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008829845.3

ORF Insert Sequence:

ATGGAGGCCC CGCTGCGGCC TGCCGCCGAC ATCCTGAGGC GGAACCCGCA GCACGACTAC 
GAGCTGGTCC AAAGGGTCGG CAGCGGTACC TACGGGGACG TCTACAAGGC CAGAAATGTA 
CATACAGGAG AATTAGCTGC AGTAAAAATC ATCAAATTGG AGCCTGGTGA TGATTTTTCT 
TTGATCCAAC AAGAAATATT TATGGTTAAA GAATGTAAAC ATTGCAACAT CGTAGCCTAC 
TTTGGGAGCT ATCTCAGTAG GGAAAAACTA TGGATTTGTA TGGAATACTG TGGTGGTGGA 
TCACTTCAAG ATATTTATCA TGTTACTGGA CCATTATCTG AATTGCAAAT AGCCTATGTG 
TGCAGAGAGA CCTTACAGGG TCTTGCCTAT TTGCATACTA AAGGCAAAAT GCATAGAGAT 
ATCAAAGGTG CAAATATCTT ATTAACAGAC CATGGTGATG TAAAATTGGC TGATTTTGGT 
GTGGCTGCAA AAATAACAGC TACCATTGCA AAGAGAAAGT CTTTCATCGG CACCCCTTAT 
TGGATGGCCC CAGAAGTTGC AGCAGTGGAG AAAAATGGTG GCTACAACCA ACTTTGTGAT 
ATCTGGGCAG TAGGAATAAC AGCAATTGAA CTTGGAGAGC TTCAGCCACC TATGTTTGAT 
CTTCATCCAA TGAGGGCTCT CTTCTTAATG TCAAAAAGTA ATTTTCAGCC TCCGAAACTA 
AAGGATAAAA CAAAATGGTC ATCAACATTC CATAATTTTG TCAAAATAGC ACTAACTAAA 
AACCCTAAAA AAAGACCAAC TGCTGAAAGA CTTCTAACGC ATACTTTCGT TGGGCAGTCA 
GGTCTGTCTC GGGCCCTAGC AGTTGAGCTG TTGGACAAAG TGAGCAATCC GGACAACCAT 
GCACATTACA CTGAAGGAGA TGACGATGAC TTTGAGCCCC ATGCAATCAT TCGTCATACC 
ATTAGATCTA CAAACAGGAA TGCCAGAGCT GAACGGACAG CTTCAGAAAT AAATTTTGAC 
AAATTACAGT TTGAACCTCC CCTGAGAAAA GAAACAGAAG CACGGGATGA AATGGGAATA 
TCATCCGACC CAAACTTTAT ATTACATTGG AATCCTTTTG TGGATGGTGC AAATACGGGC 
AAATCAACCT CAAAACGTGC AATACCGCCT CCCCTACCTC CTAAGCCAAG GATAAACAGT 
TTCCCTGAAG ACAACTTCCC AGATGATGAA AAATCATCAA CTGTCAAACG TTGCCCTGAT 
TTAGAGAACA GAGCTCCTCA AGTTCTCAGA AGACAAAGCA GCCCAAGTTG TGGTCCTGTG 
GCAGAGAGCT CCTTTATGGG AAATGGTGAT GGTATTTCAA AACTGATCAG TGAGAATACA 
GAGGGATCAG CACAAGCTCC GCAGTTACCA CGGAAAAAGG ACAAGCGAGA ATTCCCTAAA 
CCAGCTATCA ATGGCCTTCC ACCCACCCCA AAAGTACTGA TGGGAGCATG TTTTTCAAAA 
GTTTTTGATG GCTGCCCTTT GAAAATTAAT TGTGCAACAT CCTGGATACA TCCTGATACA 
AAAGATCAGT ATATCATTTT TGGAACTGAA GATGGTATTT ACACATTGAA TCTCAATGAG 
CTACACGAGG CAACTATGGA ACAGTTATTT CCACGGAAAT GTACATGGCT CTATGTTATC 
AATAATACTT TAATGTCATT ATCAGAAGGA AAATCCTTTC AGCTCTACTC TCATAATCTT 
ATTGCATTGT TTGAACAAGC CAAAAAACCA GGATTAGCTG CCCATATCCA GACTCATAGA 
TTTCCAGATC GCATTCTGCC AAGAAAGTTT GCCTTAACAA CAAAGATTCC TGATACAAAA 
GGCTGCCATA AATGTTGCAT AGTCAGAAAC CCTTATACAG GACATAAGTA CCTATGTGGA 
GCTTTGCAGT CTGGCATTGT TCTGCTTCAA TGGTATGAAC CAATGCAGAA ATTCATGCTG 
ATAAAGCACT TTGACTTTCC TTTACCAAGT CCTTTGAATG TTTTTGAGAT GCTGGTGATA 
CCAGAACAGG AATACCCGAT GGTCTGTGTA GCTATCAGTA AAGGCACAGA ATCAAATCAA 
GTAGTTCAGT TTGAAACAAT CAATTTGAAT TCTGCATCTT CATGGTTTAC AGAAATCGGT 
GCAGGCAGCC AACAGCTGGA TTCTATTCAT GTAACACAGC TGGAGAGAGA TACCGTTTTG 
GTGTGTTTAG ATAAATTTGT GAAAATTGTA AATCTACAAG GAAAACTAAA ATCAAGTAAG 
AAACTGGCCT CTGAGCTAAG TTTTGACTTT CGCATTGAAT CTGTAGTATG CCTTCAAGAC 
AGTGTGTTGG CTTTCTGGAA ACATGGGATG CAGGGTAAAA GTTTCAAATC GGATGAGGTA 
ACCCAGGAAA TTTCAGATGA AACCAGAGTT TTCCGCTTAT TGGGATCAGA CAGGGTTGTT 
GTTTTGGAAA GCAGACCAAC AGAAAACCCT ACAGCACATA GCAATCTCTA CATCTTGGCT 
GGACACGAAA ATAGTTACTA G