Iars2 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Iars2
Entrez Gene ID	103728811
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008826789.2	XP_008825011.1	isoleucine--tRNA ligase, mitochondrial
XM_008826789.3	XP_008825011.1	isoleucine--tRNA ligase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following Iars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Iars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa43856	XM_008826789.3 Latest version!	Nannospalax galili isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
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ONa43856	XM_008826789.2	Nannospalax galili isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Iars2 ( XM_008826789.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008825011.1 Nannospalax galili isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), mRNA.

Price & Availability↑

CloneID	ONa43856
Clone ID Related Accession (Same CDS sequence)	XM_008826789.2 , XM_008826789.3
Accession Version	XM_008826789.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	isoleucine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008331650.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 11, 2016 this sequence version replaced XM_008826789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGGG GGCTGCGCCG CCGCGCGTCC GGGGCGGCGG CTCTGTCAGC GGCCGGGAGC 
TGGTGGGGGC CGCCCCGCTT TCCCGGCCAC CCTGGCTGGC TGGGGACCGC CCGGAGGCTC 
GCGGGGCGCT CGGTGGCCGG GGTGGACAAC CAGCTGAGCA GCCCGAATAG CGGCAGATAC 
CGGGACACGG TGCTGCTACC GCAGACCAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCCGACATGG AGCTGGAGAT CCAGCAGAAA TGTGAGTTTG CAGAACTCTA TTCCTGGCAA 
AGAAAAAGAA AAGTAAAGAC AGAATTTTGT CTTCATGATG GACCTCCTTA TGCCAATGGC 
GACCCTCATG TTGGGCATGC TTTAAATAAG ATTCTGAAAG ACATCGCCAA TCGATACCAT 
GTGATGAGAG GCTCCAAAGT GCATTTTGTT CCTGGCTGGG ACTGTCATGG TCTGCCCATT 
GAAACAAAAG TGCTATCAGA ACTTGGTGGG AGAGCTCAGA ATCTTTCGGC TATGGAAATT 
AGAGAGAAAG CTAGATCATT TGCAAAAGCA GCCATTGAAA AACAGAAAGC AGCATTTGCT 
CGTTGGGGAG TAATGGCAGA TTGGAATAAT TGCTACTACA CATTCGATGG GAAGTATGAG 
GCCAAACAGC TGAGAATTTT TTACCAAATG TACGATCAGG GCTTGGTCTA TCGATCTTAC 
AAACCAGTGT TCTGGTCCCC CTCATCAAGA ACTGCGTTGG CTGAAGCGGA GCTTGAGTAC 
AATCCTCAGC ATGTCAGTCA GTCAGTCTAC ATAAAATTCC CTCTGTTGAA ACCTCCTCCC 
AAGCTGGCGT CTCTGACAGA TGGTTCGTCT CCTGTCAGCA TTTTAGTCTG GACCACCCAG 
CCTTGGACAG TTCCAGCTAA TCAGGCTGTT TGCTATATGC CGGAGGCAGA GTATGCTGTT 
GTGAAATGCT GCACTTCTGG AGACCGCTAC ATACTAGCTG CAGATAAAGT AGCTTCTGTT 
GCTTCTACCT TGGAAACTAC ATTTGAGGTT ACTTCAACAT TTTCAGGGGT TGATTTGGAA 
GGTGGTAGTT GTGGTCACCC TCTGGTTCCT GATAAAGCCT CTCCTCTCTT ACCTGCAACT 
CATGTGACCA TGGCCAAAGG AACAGGATTG GTTCATACAG CTCCCGCTCA TGGTATGGAA 
GATTACAGTG TAGCATCTCA ACACAGCCTA CCTATGGATT GCTTAGTGGA TGAAGGTGGA 
TTTTTCACCA GTGCTGCCGG TCCTGAACTT CAAAGCAAGG CAGTACTTGA AGAAGGAACT 
GACGTGGTTA TAAAGATGCT TCAGACTGCA GAGAATTTGT TGAAAGAAGA AAAAGTGACG 
CACAGCTATC CTTATGACTG GAGGACTAAG AAGCCAGTGG TGATCCGTGC CTGCAAGCAG 
TGGTTCGTCA ACATCGCAGA TATTAAGACC ACAGCGAAGG AGTTGTTAAA AACAGTGAAG 
TTTATTCCTG GAGCAGCATT GAACAGCATG ATCGACATGC TGGACAGGCG GCCATATTGG 
TGTATATCGA GGCAAAGAGC TTGGGGGGTT CCGATTCCTG TGTTTCATCA TAAGACAGAA 
GATGAATATT TGATCAACAG TGAAACCGTT GAGCACATAG CTAAACTAGT GGAGCAGCAC 
GGCAGTGATG TCTGGTGGAC TCTTCCCCCT GAGCAGCTTC TCCCAAAAGA AGTCTTATCT 
CAGGTTGGTG GTCCCGACTC CTTGGAATAT GTGCCAGGGC AGGATATTTT GGACATCTGG 
TTTGACAGTG GAACATCATG GTCTAGTGTT CTTCCAGGTA CTGACCAAAG AGCAGACTTG 
TACTTGGAAG GGAAAGACCA GCTTGGTGGC TGGTTTCAGT CCTCCTTACT AACCAGCGTG 
GCAGCGAGGA AGAAAGCGCC TTTTAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAAGGAGAAA AGATGTCCAA GTCCCTTGGG AATGTGGTTA ATCCTGATGT TGTCATCAAT 
GGAGGACAAG ATCAAAACAA AGAGCCTCCA TATGGTGCTG ATGTCCTGCG ATGGTGGGTG 
GCTGAATCCA ATGTATTCGC TGAAGTGACG GTTGGCCCGT CTGCACTTGG TGCTGCCAGA 
GATGACATTA ACAAGCTTAG GAACACACTC CGCTTTCTTT TGGGAAATGT GACTGGTTTT 
AACCCAGAGA CAGATTCTGT TTCCGTCAAG AATATGTATG TCATAGACCA GTATATGTTG 
CACTTACTGC AGGACTTTGC AACTAAGGTT ACTGACGCAT ACAAACAATA TGACTTTGGG 
AAAGTTGTTA GGTTGTTAAA AGCATTTTAT ACCAGAGAAC TCTCCAGCTT TTACTTCAGC 
ATAATCAAGG ATAGGCTTTA CTGTGAAAAC GAAAGCGATT CCAAACGGCG CTCCTGTCAG 
ACAGCACTAG TGGAAATTTT GGATGTGTTG GTTCGTTCTT TTGCCCCCAT TCTTCCTCAC 
TTGGCAGAAG AGGTATTCCA GCACATTCCC TATATTAAAG AACCCAAGAG TGTTTTTCGC 
ACTGGGTGGA TTAATATAAG TTCTATCTGG AAGAAGCCAG GGCTGGAGGA GGCAGTGGAG 
AGCGCGTGTG CCATGAGAGA TTCCTTCCTT GGAAGTATCC CTGGCAAAAA CCCCACAGAG 
TACGAGGTCA TCATCGTGAT CGAGCCGGGG CTGCTTTTTG AGATAATGGA GATGCTGCAG 
GCTGAGGAGA CCTCCAGCAC CTCTCAGCTG AATGAGTTAA TGATGGCCTC CCAGTCAACG 
CTGCTGGCTC AGGAACCACG GGAGAGAACT GCGGATGGAA TTGAGCTTAC AGGGACATTC 
CTCATCAATC TAGAAGGTGG TGATATTCGT GAGGAGTCTT CGTATAAAGT AATTGTCATA 
CCAGCTACCA AAGAAAAGTG CCCTCGATGT TGGAAGCACA CTGCTGAGTC TTCAGATACT 
CTGTGTCCTC GATGTGCAGA GGTTGTTGGT GGAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008825011.1
CDS	79..3117
Translation	MRWGLRRRASGAAALSAAGSWWGPPRFPGHPGWLGTARRLAGRSVAGVDNQLSSPNSGRYRDTVLLPQTSFPMKLLGRQQPDMELEIQQKCEFAELYSWQRKRKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRYHVMRGSKVHFVPGWDCHGLPIETKVLSELGGRAQNLSAMEIREKARSFAKAAIEKQKAAFARWGVMADWNNCYYTFDGKYEAKQLRIFYQMYDQGLVYRSYKPVFWSPSSRTALAEAELEYNPQHVSQSVYIKFPLLKPPPKLASLTDGSSPVSILVWTTQPWTVPANQAVCYMPEAEYAVVKCCTSGDRYILAADKVASVASTLETTFEVTSTFSGVDLEGGSCGHPLVPDKASPLLPATHVTMAKGTGLVHTAPAHGMEDYSVASQHSLPMDCLVDEGGFFTSAAGPELQSKAVLEEGTDVVIKMLQTAENLLKEEKVTHSYPYDWRTKKPVVIRACKQWFVNIADIKTTAKELLKTVKFIPGAALNSMIDMLDRRPYWCISRQRAWGVPIPVFHHKTEDEYLINSETVEHIAKLVEQHGSDVWWTLPPEQLLPKEVLSQVGGPDSLEYVPGQDILDIWFDSGTSWSSVLPGTDQRADLYLEGKDQLGGWFQSSLLTSVAARKKAPFKTVIVHGFTLGEKGEKMSKSLGNVVNPDVVINGGQDQNKEPPYGADVLRWWVAESNVFAEVTVGPSALGAARDDINKLRNTLRFLLGNVTGFNPETDSVSVKNMYVIDQYMLHLLQDFATKVTDAYKQYDFGKVVRLLKAFYTRELSSFYFSIIKDRLYCENESDSKRRSCQTALVEILDVLVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWINISSIWKKPGLEEAVESACAMRDSFLGSIPGKNPTEYEVIIVIEPGLLFEIMEMLQAEETSSTSQLNELMMASQSTLLAQEPRERTADGIELTGTFLINLEGGDIREESSYKVIVIPATKEKCPRCWKHTAESSDTLCPRCAEVVGGK

Target ORF information:

RefSeq Version	XM_008826789.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008826789.2

ORF Insert Sequence:

ATGCGTTGGG GGCTGCGCCG CCGCGCGTCC GGGGCGGCGG CTCTGTCAGC GGCCGGGAGC 
TGGTGGGGGC CGCCCCGCTT TCCCGGCCAC CCTGGCTGGC TGGGGACCGC CCGGAGGCTC 
GCGGGGCGCT CGGTGGCCGG GGTGGACAAC CAGCTGAGCA GCCCGAATAG CGGCAGATAC 
CGGGACACGG TGCTGCTACC GCAGACCAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCCGACATGG AGCTGGAGAT CCAGCAGAAA TGTGAGTTTG CAGAACTCTA TTCCTGGCAA 
AGAAAAAGAA AAGTAAAGAC AGAATTTTGT CTTCATGATG GACCTCCTTA TGCCAATGGC 
GACCCTCATG TTGGGCATGC TTTAAATAAG ATTCTGAAAG ACATCGCCAA TCGATACCAT 
GTGATGAGAG GCTCCAAAGT GCATTTTGTT CCTGGCTGGG ACTGTCATGG TCTGCCCATT 
GAAACAAAAG TGCTATCAGA ACTTGGTGGG AGAGCTCAGA ATCTTTCGGC TATGGAAATT 
AGAGAGAAAG CTAGATCATT TGCAAAAGCA GCCATTGAAA AACAGAAAGC AGCATTTGCT 
CGTTGGGGAG TAATGGCAGA TTGGAATAAT TGCTACTACA CATTCGATGG GAAGTATGAG 
GCCAAACAGC TGAGAATTTT TTACCAAATG TACGATCAGG GCTTGGTCTA TCGATCTTAC 
AAACCAGTGT TCTGGTCCCC CTCATCAAGA ACTGCGTTGG CTGAAGCGGA GCTTGAGTAC 
AATCCTCAGC ATGTCAGTCA GTCAGTCTAC ATAAAATTCC CTCTGTTGAA ACCTCCTCCC 
AAGCTGGCGT CTCTGACAGA TGGTTCGTCT CCTGTCAGCA TTTTAGTCTG GACCACCCAG 
CCTTGGACAG TTCCAGCTAA TCAGGCTGTT TGCTATATGC CGGAGGCAGA GTATGCTGTT 
GTGAAATGCT GCACTTCTGG AGACCGCTAC ATACTAGCTG CAGATAAAGT AGCTTCTGTT 
GCTTCTACCT TGGAAACTAC ATTTGAGGTT ACTTCAACAT TTTCAGGGGT TGATTTGGAA 
GGTGGTAGTT GTGGTCACCC TCTGGTTCCT GATAAAGCCT CTCCTCTCTT ACCTGCAACT 
CATGTGACCA TGGCCAAAGG AACAGGATTG GTTCATACAG CTCCCGCTCA TGGTATGGAA 
GATTACAGTG TAGCATCTCA ACACAGCCTA CCTATGGATT GCTTAGTGGA TGAAGGTGGA 
TTTTTCACCA GTGCTGCCGG TCCTGAACTT CAAAGCAAGG CAGTACTTGA AGAAGGAACT 
GACGTGGTTA TAAAGATGCT TCAGACTGCA GAGAATTTGT TGAAAGAAGA AAAAGTGACG 
CACAGCTATC CTTATGACTG GAGGACTAAG AAGCCAGTGG TGATCCGTGC CTGCAAGCAG 
TGGTTCGTCA ACATCGCAGA TATTAAGACC ACAGCGAAGG AGTTGTTAAA AACAGTGAAG 
TTTATTCCTG GAGCAGCATT GAACAGCATG ATCGACATGC TGGACAGGCG GCCATATTGG 
TGTATATCGA GGCAAAGAGC TTGGGGGGTT CCGATTCCTG TGTTTCATCA TAAGACAGAA 
GATGAATATT TGATCAACAG TGAAACCGTT GAGCACATAG CTAAACTAGT GGAGCAGCAC 
GGCAGTGATG TCTGGTGGAC TCTTCCCCCT GAGCAGCTTC TCCCAAAAGA AGTCTTATCT 
CAGGTTGGTG GTCCCGACTC CTTGGAATAT GTGCCAGGGC AGGATATTTT GGACATCTGG 
TTTGACAGTG GAACATCATG GTCTAGTGTT CTTCCAGGTA CTGACCAAAG AGCAGACTTG 
TACTTGGAAG GGAAAGACCA GCTTGGTGGC TGGTTTCAGT CCTCCTTACT AACCAGCGTG 
GCAGCGAGGA AGAAAGCGCC TTTTAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAAGGAGAAA AGATGTCCAA GTCCCTTGGG AATGTGGTTA ATCCTGATGT TGTCATCAAT 
GGAGGACAAG ATCAAAACAA AGAGCCTCCA TATGGTGCTG ATGTCCTGCG ATGGTGGGTG 
GCTGAATCCA ATGTATTCGC TGAAGTGACG GTTGGCCCGT CTGCACTTGG TGCTGCCAGA 
GATGACATTA ACAAGCTTAG GAACACACTC CGCTTTCTTT TGGGAAATGT GACTGGTTTT 
AACCCAGAGA CAGATTCTGT TTCCGTCAAG AATATGTATG TCATAGACCA GTATATGTTG 
CACTTACTGC AGGACTTTGC AACTAAGGTT ACTGACGCAT ACAAACAATA TGACTTTGGG 
AAAGTTGTTA GGTTGTTAAA AGCATTTTAT ACCAGAGAAC TCTCCAGCTT TTACTTCAGC 
ATAATCAAGG ATAGGCTTTA CTGTGAAAAC GAAAGCGATT CCAAACGGCG CTCCTGTCAG 
ACAGCACTAG TGGAAATTTT GGATGTGTTG GTTCGTTCTT TTGCCCCCAT TCTTCCTCAC 
TTGGCAGAAG AGGTATTCCA GCACATTCCC TATATTAAAG AACCCAAGAG TGTTTTTCGC 
ACTGGGTGGA TTAATATAAG TTCTATCTGG AAGAAGCCAG GGCTGGAGGA GGCAGTGGAG 
AGCGCGTGTG CCATGAGAGA TTCCTTCCTT GGAAGTATCC CTGGCAAAAA CCCCACAGAG 
TACGAGGTCA TCATCGTGAT CGAGCCGGGG CTGCTTTTTG AGATAATGGA GATGCTGCAG 
GCTGAGGAGA CCTCCAGCAC CTCTCAGCTG AATGAGTTAA TGATGGCCTC CCAGTCAACG 
CTGCTGGCTC AGGAACCACG GGAGAGAACT GCGGATGGAA TTGAGCTTAC AGGGACATTC 
CTCATCAATC TAGAAGGTGG TGATATTCGT GAGGAGTCTT CGTATAAAGT AATTGTCATA 
CCAGCTACCA AAGAAAAGTG CCCTCGATGT TGGAAGCACA CTGCTGAGTC TTCAGATACT 
CTGTGTCCTC GATGTGCAGA GGTTGTTGGT GGAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iars2 ( XM_008826789.3 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008825011.1 Nannospalax galili isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), mRNA.

Price & Availability↑

CloneID	ONa43856
Clone ID Related Accession (Same CDS sequence)	XM_008826789.2 , XM_008826789.3
Accession Version	XM_008826789.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	isoleucine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008331650.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008826789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGGG GGCTGCGCCG CCGCGCGTCC GGGGCGGCGG CTCTGTCAGC GGCCGGGAGC 
TGGTGGGGGC CGCCCCGCTT TCCCGGCCAC CCTGGCTGGC TGGGGACCGC CCGGAGGCTC 
GCGGGGCGCT CGGTGGCCGG GGTGGACAAC CAGCTGAGCA GCCCGAATAG CGGCAGATAC 
CGGGACACGG TGCTGCTACC GCAGACCAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCCGACATGG AGCTGGAGAT CCAGCAGAAA TGTGAGTTTG CAGAACTCTA TTCCTGGCAA 
AGAAAAAGAA AAGTAAAGAC AGAATTTTGT CTTCATGATG GACCTCCTTA TGCCAATGGC 
GACCCTCATG TTGGGCATGC TTTAAATAAG ATTCTGAAAG ACATCGCCAA TCGATACCAT 
GTGATGAGAG GCTCCAAAGT GCATTTTGTT CCTGGCTGGG ACTGTCATGG TCTGCCCATT 
GAAACAAAAG TGCTATCAGA ACTTGGTGGG AGAGCTCAGA ATCTTTCGGC TATGGAAATT 
AGAGAGAAAG CTAGATCATT TGCAAAAGCA GCCATTGAAA AACAGAAAGC AGCATTTGCT 
CGTTGGGGAG TAATGGCAGA TTGGAATAAT TGCTACTACA CATTCGATGG GAAGTATGAG 
GCCAAACAGC TGAGAATTTT TTACCAAATG TACGATCAGG GCTTGGTCTA TCGATCTTAC 
AAACCAGTGT TCTGGTCCCC CTCATCAAGA ACTGCGTTGG CTGAAGCGGA GCTTGAGTAC 
AATCCTCAGC ATGTCAGTCA GTCAGTCTAC ATAAAATTCC CTCTGTTGAA ACCTCCTCCC 
AAGCTGGCGT CTCTGACAGA TGGTTCGTCT CCTGTCAGCA TTTTAGTCTG GACCACCCAG 
CCTTGGACAG TTCCAGCTAA TCAGGCTGTT TGCTATATGC CGGAGGCAGA GTATGCTGTT 
GTGAAATGCT GCACTTCTGG AGACCGCTAC ATACTAGCTG CAGATAAAGT AGCTTCTGTT 
GCTTCTACCT TGGAAACTAC ATTTGAGGTT ACTTCAACAT TTTCAGGGGT TGATTTGGAA 
GGTGGTAGTT GTGGTCACCC TCTGGTTCCT GATAAAGCCT CTCCTCTCTT ACCTGCAACT 
CATGTGACCA TGGCCAAAGG AACAGGATTG GTTCATACAG CTCCCGCTCA TGGTATGGAA 
GATTACAGTG TAGCATCTCA ACACAGCCTA CCTATGGATT GCTTAGTGGA TGAAGGTGGA 
TTTTTCACCA GTGCTGCCGG TCCTGAACTT CAAAGCAAGG CAGTACTTGA AGAAGGAACT 
GACGTGGTTA TAAAGATGCT TCAGACTGCA GAGAATTTGT TGAAAGAAGA AAAAGTGACG 
CACAGCTATC CTTATGACTG GAGGACTAAG AAGCCAGTGG TGATCCGTGC CTGCAAGCAG 
TGGTTCGTCA ACATCGCAGA TATTAAGACC ACAGCGAAGG AGTTGTTAAA AACAGTGAAG 
TTTATTCCTG GAGCAGCATT GAACAGCATG ATCGACATGC TGGACAGGCG GCCATATTGG 
TGTATATCGA GGCAAAGAGC TTGGGGGGTT CCGATTCCTG TGTTTCATCA TAAGACAGAA 
GATGAATATT TGATCAACAG TGAAACCGTT GAGCACATAG CTAAACTAGT GGAGCAGCAC 
GGCAGTGATG TCTGGTGGAC TCTTCCCCCT GAGCAGCTTC TCCCAAAAGA AGTCTTATCT 
CAGGTTGGTG GTCCCGACTC CTTGGAATAT GTGCCAGGGC AGGATATTTT GGACATCTGG 
TTTGACAGTG GAACATCATG GTCTAGTGTT CTTCCAGGTA CTGACCAAAG AGCAGACTTG 
TACTTGGAAG GGAAAGACCA GCTTGGTGGC TGGTTTCAGT CCTCCTTACT AACCAGCGTG 
GCAGCGAGGA AGAAAGCGCC TTTTAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAAGGAGAAA AGATGTCCAA GTCCCTTGGG AATGTGGTTA ATCCTGATGT TGTCATCAAT 
GGAGGACAAG ATCAAAACAA AGAGCCTCCA TATGGTGCTG ATGTCCTGCG ATGGTGGGTG 
GCTGAATCCA ATGTATTCGC TGAAGTGACG GTTGGCCCGT CTGCACTTGG TGCTGCCAGA 
GATGACATTA ACAAGCTTAG GAACACACTC CGCTTTCTTT TGGGAAATGT GACTGGTTTT 
AACCCAGAGA CAGATTCTGT TTCCGTCAAG AATATGTATG TCATAGACCA GTATATGTTG 
CACTTACTGC AGGACTTTGC AACTAAGGTT ACTGACGCAT ACAAACAATA TGACTTTGGG 
AAAGTTGTTA GGTTGTTAAA AGCATTTTAT ACCAGAGAAC TCTCCAGCTT TTACTTCAGC 
ATAATCAAGG ATAGGCTTTA CTGTGAAAAC GAAAGCGATT CCAAACGGCG CTCCTGTCAG 
ACAGCACTAG TGGAAATTTT GGATGTGTTG GTTCGTTCTT TTGCCCCCAT TCTTCCTCAC 
TTGGCAGAAG AGGTATTCCA GCACATTCCC TATATTAAAG AACCCAAGAG TGTTTTTCGC 
ACTGGGTGGA TTAATATAAG TTCTATCTGG AAGAAGCCAG GGCTGGAGGA GGCAGTGGAG 
AGCGCGTGTG CCATGAGAGA TTCCTTCCTT GGAAGTATCC CTGGCAAAAA CCCCACAGAG 
TACGAGGTCA TCATCGTGAT CGAGCCGGGG CTGCTTTTTG AGATAATGGA GATGCTGCAG 
GCTGAGGAGA CCTCCAGCAC CTCTCAGCTG AATGAGTTAA TGATGGCCTC CCAGTCAACG 
CTGCTGGCTC AGGAACCACG GGAGAGAACT GCGGATGGAA TTGAGCTTAC AGGGACATTC 
CTCATCAATC TAGAAGGTGG TGATATTCGT GAGGAGTCTT CGTATAAAGT AATTGTCATA 
CCAGCTACCA AAGAAAAGTG CCCTCGATGT TGGAAGCACA CTGCTGAGTC TTCAGATACT 
CTGTGTCCTC GATGTGCAGA GGTTGTTGGT GGAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008825011.1
CDS	120..3158
Translation	MRWGLRRRASGAAALSAAGSWWGPPRFPGHPGWLGTARRLAGRSVAGVDNQLSSPNSGRYRDTVLLPQTSFPMKLLGRQQPDMELEIQQKCEFAELYSWQRKRKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRYHVMRGSKVHFVPGWDCHGLPIETKVLSELGGRAQNLSAMEIREKARSFAKAAIEKQKAAFARWGVMADWNNCYYTFDGKYEAKQLRIFYQMYDQGLVYRSYKPVFWSPSSRTALAEAELEYNPQHVSQSVYIKFPLLKPPPKLASLTDGSSPVSILVWTTQPWTVPANQAVCYMPEAEYAVVKCCTSGDRYILAADKVASVASTLETTFEVTSTFSGVDLEGGSCGHPLVPDKASPLLPATHVTMAKGTGLVHTAPAHGMEDYSVASQHSLPMDCLVDEGGFFTSAAGPELQSKAVLEEGTDVVIKMLQTAENLLKEEKVTHSYPYDWRTKKPVVIRACKQWFVNIADIKTTAKELLKTVKFIPGAALNSMIDMLDRRPYWCISRQRAWGVPIPVFHHKTEDEYLINSETVEHIAKLVEQHGSDVWWTLPPEQLLPKEVLSQVGGPDSLEYVPGQDILDIWFDSGTSWSSVLPGTDQRADLYLEGKDQLGGWFQSSLLTSVAARKKAPFKTVIVHGFTLGEKGEKMSKSLGNVVNPDVVINGGQDQNKEPPYGADVLRWWVAESNVFAEVTVGPSALGAARDDINKLRNTLRFLLGNVTGFNPETDSVSVKNMYVIDQYMLHLLQDFATKVTDAYKQYDFGKVVRLLKAFYTRELSSFYFSIIKDRLYCENESDSKRRSCQTALVEILDVLVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWINISSIWKKPGLEEAVESACAMRDSFLGSIPGKNPTEYEVIIVIEPGLLFEIMEMLQAEETSSTSQLNELMMASQSTLLAQEPRERTADGIELTGTFLINLEGGDIREESSYKVIVIPATKEKCPRCWKHTAESSDTLCPRCAEVVGGK

Target ORF information:

RefSeq Version	XM_008826789.3
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008826789.3

ORF Insert Sequence:

ATGCGTTGGG GGCTGCGCCG CCGCGCGTCC GGGGCGGCGG CTCTGTCAGC GGCCGGGAGC 
TGGTGGGGGC CGCCCCGCTT TCCCGGCCAC CCTGGCTGGC TGGGGACCGC CCGGAGGCTC 
GCGGGGCGCT CGGTGGCCGG GGTGGACAAC CAGCTGAGCA GCCCGAATAG CGGCAGATAC 
CGGGACACGG TGCTGCTACC GCAGACCAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCCGACATGG AGCTGGAGAT CCAGCAGAAA TGTGAGTTTG CAGAACTCTA TTCCTGGCAA 
AGAAAAAGAA AAGTAAAGAC AGAATTTTGT CTTCATGATG GACCTCCTTA TGCCAATGGC 
GACCCTCATG TTGGGCATGC TTTAAATAAG ATTCTGAAAG ACATCGCCAA TCGATACCAT 
GTGATGAGAG GCTCCAAAGT GCATTTTGTT CCTGGCTGGG ACTGTCATGG TCTGCCCATT 
GAAACAAAAG TGCTATCAGA ACTTGGTGGG AGAGCTCAGA ATCTTTCGGC TATGGAAATT 
AGAGAGAAAG CTAGATCATT TGCAAAAGCA GCCATTGAAA AACAGAAAGC AGCATTTGCT 
CGTTGGGGAG TAATGGCAGA TTGGAATAAT TGCTACTACA CATTCGATGG GAAGTATGAG 
GCCAAACAGC TGAGAATTTT TTACCAAATG TACGATCAGG GCTTGGTCTA TCGATCTTAC 
AAACCAGTGT TCTGGTCCCC CTCATCAAGA ACTGCGTTGG CTGAAGCGGA GCTTGAGTAC 
AATCCTCAGC ATGTCAGTCA GTCAGTCTAC ATAAAATTCC CTCTGTTGAA ACCTCCTCCC 
AAGCTGGCGT CTCTGACAGA TGGTTCGTCT CCTGTCAGCA TTTTAGTCTG GACCACCCAG 
CCTTGGACAG TTCCAGCTAA TCAGGCTGTT TGCTATATGC CGGAGGCAGA GTATGCTGTT 
GTGAAATGCT GCACTTCTGG AGACCGCTAC ATACTAGCTG CAGATAAAGT AGCTTCTGTT 
GCTTCTACCT TGGAAACTAC ATTTGAGGTT ACTTCAACAT TTTCAGGGGT TGATTTGGAA 
GGTGGTAGTT GTGGTCACCC TCTGGTTCCT GATAAAGCCT CTCCTCTCTT ACCTGCAACT 
CATGTGACCA TGGCCAAAGG AACAGGATTG GTTCATACAG CTCCCGCTCA TGGTATGGAA 
GATTACAGTG TAGCATCTCA ACACAGCCTA CCTATGGATT GCTTAGTGGA TGAAGGTGGA 
TTTTTCACCA GTGCTGCCGG TCCTGAACTT CAAAGCAAGG CAGTACTTGA AGAAGGAACT 
GACGTGGTTA TAAAGATGCT TCAGACTGCA GAGAATTTGT TGAAAGAAGA AAAAGTGACG 
CACAGCTATC CTTATGACTG GAGGACTAAG AAGCCAGTGG TGATCCGTGC CTGCAAGCAG 
TGGTTCGTCA ACATCGCAGA TATTAAGACC ACAGCGAAGG AGTTGTTAAA AACAGTGAAG 
TTTATTCCTG GAGCAGCATT GAACAGCATG ATCGACATGC TGGACAGGCG GCCATATTGG 
TGTATATCGA GGCAAAGAGC TTGGGGGGTT CCGATTCCTG TGTTTCATCA TAAGACAGAA 
GATGAATATT TGATCAACAG TGAAACCGTT GAGCACATAG CTAAACTAGT GGAGCAGCAC 
GGCAGTGATG TCTGGTGGAC TCTTCCCCCT GAGCAGCTTC TCCCAAAAGA AGTCTTATCT 
CAGGTTGGTG GTCCCGACTC CTTGGAATAT GTGCCAGGGC AGGATATTTT GGACATCTGG 
TTTGACAGTG GAACATCATG GTCTAGTGTT CTTCCAGGTA CTGACCAAAG AGCAGACTTG 
TACTTGGAAG GGAAAGACCA GCTTGGTGGC TGGTTTCAGT CCTCCTTACT AACCAGCGTG 
GCAGCGAGGA AGAAAGCGCC TTTTAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAAGGAGAAA AGATGTCCAA GTCCCTTGGG AATGTGGTTA ATCCTGATGT TGTCATCAAT 
GGAGGACAAG ATCAAAACAA AGAGCCTCCA TATGGTGCTG ATGTCCTGCG ATGGTGGGTG 
GCTGAATCCA ATGTATTCGC TGAAGTGACG GTTGGCCCGT CTGCACTTGG TGCTGCCAGA 
GATGACATTA ACAAGCTTAG GAACACACTC CGCTTTCTTT TGGGAAATGT GACTGGTTTT 
AACCCAGAGA CAGATTCTGT TTCCGTCAAG AATATGTATG TCATAGACCA GTATATGTTG 
CACTTACTGC AGGACTTTGC AACTAAGGTT ACTGACGCAT ACAAACAATA TGACTTTGGG 
AAAGTTGTTA GGTTGTTAAA AGCATTTTAT ACCAGAGAAC TCTCCAGCTT TTACTTCAGC 
ATAATCAAGG ATAGGCTTTA CTGTGAAAAC GAAAGCGATT CCAAACGGCG CTCCTGTCAG 
ACAGCACTAG TGGAAATTTT GGATGTGTTG GTTCGTTCTT TTGCCCCCAT TCTTCCTCAC 
TTGGCAGAAG AGGTATTCCA GCACATTCCC TATATTAAAG AACCCAAGAG TGTTTTTCGC 
ACTGGGTGGA TTAATATAAG TTCTATCTGG AAGAAGCCAG GGCTGGAGGA GGCAGTGGAG 
AGCGCGTGTG CCATGAGAGA TTCCTTCCTT GGAAGTATCC CTGGCAAAAA CCCCACAGAG 
TACGAGGTCA TCATCGTGAT CGAGCCGGGG CTGCTTTTTG AGATAATGGA GATGCTGCAG 
GCTGAGGAGA CCTCCAGCAC CTCTCAGCTG AATGAGTTAA TGATGGCCTC CCAGTCAACG 
CTGCTGGCTC AGGAACCACG GGAGAGAACT GCGGATGGAA TTGAGCTTAC AGGGACATTC 
CTCATCAATC TAGAAGGTGG TGATATTCGT GAGGAGTCTT CGTATAAAGT AATTGTCATA 
CCAGCTACCA AAGAAAAGTG CCCTCGATGT TGGAAGCACA CTGCTGAGTC TTCAGATACT 
CTGTGTCCTC GATGTGCAGA GGTTGTTGGT GGAAAGTAG