Srd5a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srd5a2
Entrez Gene ID	94224
Full Name	steroid 5 alpha-reductase 2
Synonyms	5ART2,S5AR 2
General protein information	Preferred Names steroid 5 alpha-reductase 2 Names 3-oxo-5-alpha-steroid 4-dehydrogenase 2 5 alpha-SR2 SR type 2 steroid 5 alpha reductase type2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_053188.3	NP_444418.1	3-oxo-5-alpha-steroid 4-dehydrogenase 2
NM_053188.2	NP_444418.1	3-oxo-5-alpha-steroid 4-dehydrogenase 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SRD5A2	NP_000339.2
Rattus norvegicus (Norway rat)	Srd5a2	NP_073202.1
Mus musculus (house mouse)	Srd5a2	NP_444418.1
Gallus gallus (chicken)	SRD5A2	XP_001235447.1
Caenorhabditis elegans (roundworm)	CELE_ZK1251.3	NP_501719.1
	SRD5A2	XP_001105329.1
Canis lupus familiaris (dog)	SRD5A2	XP_532922.2
Bos taurus (cattle)	SRD5A2	NP_001192866.1
Danio rerio (zebrafish)	srd5a2a	NP_001017703.1
Caenorhabditis elegans (roundworm)	F19H6.4	NP_510077.1
Caenorhabditis elegans (roundworm)	CELE_F42F12.3	NP_510071.1
Xenopus tropicalis (tropical clawed frog)	srd5a2	NP_001017113.1
Pan troglodytes (chimpanzee)	SRD5A2	XP_003309033.1
Danio rerio (zebrafish)	srd5a2b	NP_001017693.1

Gene Ontology
Bibliography

Related articles in PubMed

5?-reductase 1 mRNA levels are positively correlated with TRAMP mouse prostate most severe lesion scores.
Opoku-Acheampong AB, Henningson JN, Beck AP, Lindshield BL
PloS one12(5)e0175874(2017)

Sex chromosome complement determines sex differences in aromatase expression and regulation in the stria terminalis and anterior amygdala of the developing mouse brain.
Cisternas CD, Tome K, Caeiro XE, Dadam FM, Garcia-Segura LM, Cambiasso MJ
Molecular and cellular endocrinology41499-110(2015 Oct)

Loss of 5?-reductase type 1 accelerates the development of hepatic steatosis but protects against hepatocellular carcinoma in male mice.
Dowman JK, Hopkins LJ, Reynolds GM, Armstrong MJ, Nasiri M, Nikolaou N, van Houten EL, Visser JA, Morgan SA, Lavery GG, Oprescu A, H?bscher SG, Newsome PN, Tomlinson JW
Endocrinology154(12)4536-47(2013 Dec)

Wnt inhibitory factor 1 (Wif1) is regulated by androgens and enhances androgen-dependent prostate development.
Keil KP, Mehta V, Branam AM, Abler LL, Buresh-Stiemke RA, Joshi PS, Schmitz CT, Marker PC, Vezina CM
Endocrinology153(12)6091-103(2012 Dec)

A role for selective androgen response elements in the development of the epididymis and the androgen control of the 5? reductase II gene.
Kerkhofs S, Dubois V, De Gendt K, Helsen C, Clinckemalie L, Spans L, Schuit F, Boonen S, Vanderschueren D, Saunders PT, Verhoeven G, Claessens F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(10)4360-72(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prostate 5aR1 levels were positively correlated with adjusted prostate most severe lesion scores. Downregulation of androgen receptor and 5alpha-reductase 2, along with upregulation of 5a-reductase 1 in tumors may promote prostatic intraepithelial neoplasia and prostate cancer development in TRAMP mice
Title: 5?-reductase 1 mRNA levels are positively correlated with TRAMP mouse prostate most severe lesion scores.

The induction of Srd5a2 through SREBP-2 provides a mechanistic explanation for why even though statin therapy is effective in reducing cholesterol levels in treating hypercholesterolemia it does not compromise androgen production in clinical studies.
Title: Regulation of steroid 5-alpha reductase type 2 (Srd5a2) by sterol regulatory element binding proteins and statin.

observed an abnormally high expression levels of type 2 5alpha-reductase and 20alpha-HSD in the male and female adrenals, respectively, thus indirectly suggesting new roles for these sex-specific enzymes
Title: Quantitative appreciation of steroidogenic gene expression in mouse tissues: new roles for type 2 5alpha-reductase, 20alpha-hydroxysteroid dehydrogenase and estrogen sulfotransferase.

molecular cloning & transcriptional activation of the gene
Title: Transcriptional regulation of the mouse steroid 5alpha-reductase type II gene by progesterone in brain.

The following Srd5a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srd5a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09326	NM_053188.3 Latest version!	Mus musculus steroid 5 alpha-reductase 2 (Srd5a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu09326	NM_053188.2	Mus musculus steroid 5 alpha-reductase 2 (Srd5a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srd5a2 ( NM_053188.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444418.1 Mus musculus steroid 5 alpha-reductase 2 (Srd5a2), mRNA.

Price & Availability↑

CloneID	OMu09326
Clone ID Related Accession (Same CDS sequence)	NM_053188.3 , NM_053188.2
Accession Version	NM_053188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Mus musculus(house mouse)
Product	3-oxo-5-alpha-steroid 4-dehydrogenase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT025731.18. On Jun 24, 2019 this sequence version replaced NM_053188.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125510.1, AK138946.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGATTG TGTGCCATCA GGTCCCGGTG CTGGCAGGTA GCGCCACATT GGCCACCATG 
GGGACCCTGA TCCTGTGCTT TGGGAAACCC GCCAGTTACG GGAAACACAG CGAGAGTGTG 
TCGTCGGGAG TTCCCCTCCT GCCCGCACGC ATAGCCTGGT TCCTGCAGGA GCTGCCTTCC 
TTTGTGGTGT CGGTAGGGAT GCTCGCTTGG CAGCCGCGCT CCCTCTTTGG ACCTCCGGGG 
AATGTGCTGC TGGGTCTCTT CTCCGCACAT TACTTCCACA GGACATTTAT TTACTCATTG 
CTCACCAGAG GCAGGCCTTT ATCAGCGGTG ATCTTTCTGA AAGCCACTGC CTTCTGCATA 
GGGAACGGAC TCCTGCAAGC CTATTACCTG GTTTATTGCG CGGAATACCC TGAGGAGTGG 
TACACAGACA TGCGGTTTAG CGTCGGTGTC TTCTTCTTTA TTCTGGGGAT GGGGATCAAC 
ATCCACAGTG ACTGCATGCT GCGCCAGCTC AGGAAGCCCG GAGAGGTCAT CTACAGGATC 
CCACAAGGTG GCTTGTTTAC GTATGTGTCT GGAGCCAATT TCCTGGGCGA GATTATTGAA 
TGGATGGGCT ACGCTTTGGC CACTTGGTCT GTTCCAGCCT TTGCTTTTGC ATTTTTCACA 
CTTTGTTTCC TGGGGATGCA AGCTTTTTAC CACCACAGGT TCTACCTCAA GATGTTTAAG 
GATTACCCCA AATCTAGGAA AGCCCTCATT CCATTCATCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444418.1
CDS	34..798
Translation	MPIVCHQVPVLAGSATLATMGTLILCFGKPASYGKHSESVSSGVPLLPARIAWFLQELPSFVVSVGMLAWQPRSLFGPPGNVLLGLFSAHYFHRTFIYSLLTRGRPLSAVIFLKATAFCIGNGLLQAYYLVYCAEYPEEWYTDMRFSVGVFFFILGMGINIHSDCMLRQLRKPGEVIYRIPQGGLFTYVSGANFLGEIIEWMGYALATWSVPAFAFAFFTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF

Target ORF information:

RefSeq Version	NM_053188.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid 5 alpha-reductase 2 (Srd5a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053188.3

ORF Insert Sequence:

ATGCCGATTG TGTGCCATCA GGTCCCGGTG CTGGCAGGTA GCGCCACATT GGCCACCATG 
GGGACCCTGA TCCTGTGCTT TGGGAAACCC GCCAGTTACG GGAAACACAG CGAGAGTGTG 
TCGTCGGGAG TTCCCCTCCT GCCCGCACGC ATAGCCTGGT TCCTGCAGGA GCTGCCTTCC 
TTTGTGGTGT CGGTAGGGAT GCTCGCTTGG CAGCCGCGCT CCCTCTTTGG ACCTCCGGGG 
AATGTGCTGC TGGGTCTCTT CTCCGCACAT TACTTCCACA GGACATTTAT TTACTCATTG 
CTCACCAGAG GCAGGCCTTT ATCAGCGGTG ATCTTTCTGA AAGCCACTGC CTTCTGCATA 
GGGAACGGAC TCCTGCAAGC CTATTACCTG GTTTATTGCG CGGAATACCC TGAGGAGTGG 
TACACAGACA TGCGGTTTAG CGTCGGTGTC TTCTTCTTTA TTCTGGGGAT GGGGATCAAC 
ATCCACAGTG ACTGCATGCT GCGCCAGCTC AGGAAGCCCG GAGAGGTCAT CTACAGGATC 
CCACAAGGTG GCTTGTTTAC GTATGTGTCT GGAGCCAATT TCCTGGGCGA GATTATTGAA 
TGGATGGGCT ACGCTTTGGC CACTTGGTCT GTTCCAGCCT TTGCTTTTGC ATTTTTCACA 
CTTTGTTTCC TGGGGATGCA AGCTTTTTAC CACCACAGGT TCTACCTCAA GATGTTTAAG 
GATTACCCCA AATCTAGGAA AGCCCTCATT CCATTCATCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srd5a2 ( NM_053188.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444418.1 Mus musculus steroid 5 alpha-reductase 2 (Srd5a2), mRNA.

Price & Availability↑

CloneID	OMu09326
Clone ID Related Accession (Same CDS sequence)	NM_053188.3 , NM_053188.2
Accession Version	NM_053188.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	3-oxo-5-alpha-steroid 4-dehydrogenase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT025731.18. On Nov 17, 2006 this sequence version replaced NM_053188.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049456.1, BC125510.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGCCGATTG TGTGCCATCA GGTCCCGGTG CTGGCAGGTA GCGCCACATT GGCCACCATG 
GGGACCCTGA TCCTGTGCTT TGGGAAACCC GCCAGTTACG GGAAACACAG CGAGAGTGTG 
TCGTCGGGAG TTCCCCTCCT GCCCGCACGC ATAGCCTGGT TCCTGCAGGA GCTGCCTTCC 
TTTGTGGTGT CGGTAGGGAT GCTCGCTTGG CAGCCGCGCT CCCTCTTTGG ACCTCCGGGG 
AATGTGCTGC TGGGTCTCTT CTCCGCACAT TACTTCCACA GGACATTTAT TTACTCATTG 
CTCACCAGAG GCAGGCCTTT ATCAGCGGTG ATCTTTCTGA AAGCCACTGC CTTCTGCATA 
GGGAACGGAC TCCTGCAAGC CTATTACCTG GTTTATTGCG CGGAATACCC TGAGGAGTGG 
TACACAGACA TGCGGTTTAG CGTCGGTGTC TTCTTCTTTA TTCTGGGGAT GGGGATCAAC 
ATCCACAGTG ACTGCATGCT GCGCCAGCTC AGGAAGCCCG GAGAGGTCAT CTACAGGATC 
CCACAAGGTG GCTTGTTTAC GTATGTGTCT GGAGCCAATT TCCTGGGCGA GATTATTGAA 
TGGATGGGCT ACGCTTTGGC CACTTGGTCT GTTCCAGCCT TTGCTTTTGC ATTTTTCACA 
CTTTGTTTCC TGGGGATGCA AGCTTTTTAC CACCACAGGT TCTACCTCAA GATGTTTAAG 
GATTACCCCA AATCTAGGAA AGCCCTCATT CCATTCATCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444418.1
CDS	90..854
Translation	MPIVCHQVPVLAGSATLATMGTLILCFGKPASYGKHSESVSSGVPLLPARIAWFLQELPSFVVSVGMLAWQPRSLFGPPGNVLLGLFSAHYFHRTFIYSLLTRGRPLSAVIFLKATAFCIGNGLLQAYYLVYCAEYPEEWYTDMRFSVGVFFFILGMGINIHSDCMLRQLRKPGEVIYRIPQGGLFTYVSGANFLGEIIEWMGYALATWSVPAFAFAFFTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF

Target ORF information:

RefSeq Version	NM_053188.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid 5 alpha-reductase 2 (Srd5a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053188.2

ORF Insert Sequence:

ATGCCGATTG TGTGCCATCA GGTCCCGGTG CTGGCAGGTA GCGCCACATT GGCCACCATG 
GGGACCCTGA TCCTGTGCTT TGGGAAACCC GCCAGTTACG GGAAACACAG CGAGAGTGTG 
TCGTCGGGAG TTCCCCTCCT GCCCGCACGC ATAGCCTGGT TCCTGCAGGA GCTGCCTTCC 
TTTGTGGTGT CGGTAGGGAT GCTCGCTTGG CAGCCGCGCT CCCTCTTTGG ACCTCCGGGG 
AATGTGCTGC TGGGTCTCTT CTCCGCACAT TACTTCCACA GGACATTTAT TTACTCATTG 
CTCACCAGAG GCAGGCCTTT ATCAGCGGTG ATCTTTCTGA AAGCCACTGC CTTCTGCATA 
GGGAACGGAC TCCTGCAAGC CTATTACCTG GTTTATTGCG CGGAATACCC TGAGGAGTGG 
TACACAGACA TGCGGTTTAG CGTCGGTGTC TTCTTCTTTA TTCTGGGGAT GGGGATCAAC 
ATCCACAGTG ACTGCATGCT GCGCCAGCTC AGGAAGCCCG GAGAGGTCAT CTACAGGATC 
CCACAAGGTG GCTTGTTTAC GTATGTGTCT GGAGCCAATT TCCTGGGCGA GATTATTGAA 
TGGATGGGCT ACGCTTTGGC CACTTGGTCT GTTCCAGCCT TTGCTTTTGC ATTTTTCACA 
CTTTGTTTCC TGGGGATGCA AGCTTTTTAC CACCACAGGT TCTACCTCAA GATGTTTAAG 
GATTACCCCA AATCTAGGAA AGCCCTCATT CCATTCATCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

5?-reductase 1 mRNA levels are positively correlated with TRAMP mouse prostate most severe lesion scores.
PloS one12(5)e0175874(2017)
Opoku-Acheampong AB,Henningson JN,Beck AP,Lindshield BL

Sex chromosome complement determines sex differences in aromatase expression and regulation in the stria terminalis and anterior amygdala of the developing mouse brain.
Molecular and cellular endocrinology41499-110(2015 Oct)
Cisternas CD,Tome K,Caeiro XE,Dadam FM,Garcia-Segura LM,Cambiasso MJ

Loss of 5?-reductase type 1 accelerates the development of hepatic steatosis but protects against hepatocellular carcinoma in male mice.
Endocrinology154(12)4536-47(2013 Dec)
Dowman JK,Hopkins LJ,Reynolds GM,Armstrong MJ,Nasiri M,Nikolaou N,van Houten EL,Visser JA,Morgan SA,Lavery GG,Oprescu A,H?bscher SG,Newsome PN,Tomlinson JW

Wnt inhibitory factor 1 (Wif1) is regulated by androgens and enhances androgen-dependent prostate development.
Endocrinology153(12)6091-103(2012 Dec)
Keil KP,Mehta V,Branam AM,Abler LL,Buresh-Stiemke RA,Joshi PS,Schmitz CT,Marker PC,Vezina CM

A role for selective androgen response elements in the development of the epididymis and the androgen control of the 5? reductase II gene.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(10)4360-72(2012 Oct)
Kerkhofs S,Dubois V,De Gendt K,Helsen C,Clinckemalie L,Spans L,Schuit F,Boonen S,Vanderschueren D,Saunders PT,Verhoeven G,Claessens F

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Srd5a2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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