Nipa2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nipa2
Entrez Gene ID	93790
Full Name	non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human)
Synonyms	2600017P10Rik,3830408P04Rik,AB041581
General protein information	Preferred Names non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) Names magnesium transporter NIPA2 non-imprinted in Prader-Willi/Angelman syndrome region protein 2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006541324.3	XP_006541387.1	magnesium transporter NIPA2 isoform X1
XM_006541324.4	XP_006541387.1	magnesium transporter NIPA2 isoform X1
NM_001256133.1	NP_001243062.1	magnesium transporter NIPA2 isoform 2
NM_001256132.1	NP_001243061.1	magnesium transporter NIPA2 isoform 1
XM_006541324.2	XP_006541387.1	magnesium transporter NIPA2 isoform X1
NM_001256130.1	NP_001243059.1	magnesium transporter NIPA2 isoform 1
NM_001256131.1	NP_001243060.1	magnesium transporter NIPA2 isoform 1
NM_023647.6	NP_076136.2	magnesium transporter NIPA2 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NIPA2	XP_005618264.1
Bos taurus (cattle)	NIPA2	NP_001029470.1
Rattus norvegicus (Norway rat)	Nipa2	NP_001100988.1
Neurospora crassa	NCU04457	XP_957026.2
	NIPA2	XP_001106265.1
Mus musculus (house mouse)	Nipa2	NP_076136.2
Gallus gallus (chicken)	NIPA2	NP_001025980.1
Drosophila melanogaster (fruit fly)	spict	NP_609586.1
Danio rerio (zebrafish)	nipa2	NP_998290.1
Homo sapiens (human)	NIPA2	NP_001008860.1
Pan troglodytes (chimpanzee)	NIPA2	XP_003314615.2
Oryza sativa (rice)	Os05g0513400	NP_001056027.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009838	XP_318951.3
Caenorhabditis elegans (roundworm)	nipa-1	NP_001122733.1
Magnaporthe oryzae (rice blast fungus)	MGG_08036	XP_003714989.1
Xenopus tropicalis (tropical clawed frog)	nipa2	NP_001011172.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional characterization of NIPA2, a selective Mg2+ transporter.
Goytain A, Hines RM, Quamme GA
American journal of physiology. Cell physiology295(4)C944-53(2008 Oct)

Identification of four highly conserved genes between breakpoint hotspots BP1 and BP2 of the Prader-Willi/Angelman syndromes deletion region that have undergone evolutionary transposition mediated by flanking duplicons.
Chai JH, Locke DP, Greally JM, Knoll JH, Ohta T, Dunai J, Yavor A, Eichler EE, Nicholls RD
American journal of human genetics73(4)898-925(2003 Oct)

Protein-protein interaction panel using mouse full-length cDNAs.
Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y
Genome research11(10)1758-65(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The isolation and characterization of Nipa2 in mouse distal convoluted tubule cells are reported.
Title: Functional characterization of NIPA2, a selective Mg2+ transporter.

indentification on mouse chromosome 7C
Title: Identification of four highly conserved genes between breakpoint hotspots BP1 and BP2 of the Prader-Willi/Angelman syndromes deletion region that have undergone evolutionary transposition mediated by flanking duplicons.

The following Nipa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nipa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05097	NM_001256130.1 Latest version!	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu05097	NM_001256132.1 Latest version!	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu05097	NM_023647.6 Latest version!	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu05097	NM_001256131.1 Latest version!	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu06046	NM_001256133.1 Latest version!	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu05097	XM_006541324.4 Latest version!	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu05097	XM_006541324.3	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu05097	XM_006541324.2	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nipa2 ( NM_001256132.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243061.1 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu05097
Clone ID Related Accession (Same CDS sequence)	XM_006541324.3 , XM_006541324.4 , NM_001256132.1 , XM_006541324.2 , NM_001256130.1 , NM_001256131.1 , NM_023647.6
Accession Version	NM_001256132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	magnesium transporter NIPA2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK041427.1, AK008106.1, BK001121.1 and AI119710.1. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1-4 encode the same isoform (1). ##Evidence-Data-START## CDS exon combination :: BK001121.1, SRR1660821.88459.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243061.1
CDS	310..1389
Translation	MSLGRGKYDFYIGLGLAMTSSIFIGGSFILKKKGLLRLARKGSMRAGQGGHAYLKEWLWWAGLLSMGAGEVANFAAYAFAPATLVTPLGALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATFVVIVALIFIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELLAGKPVLQHPLAWILLFSLVVCVSTQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAFKDVSFSLASLPVSFRKDEKAMNGNLSSMYEVLNNNEDDLPCGIEHTGENISRRNGNLPSF

Target ORF information:

RefSeq Version	NM_001256132.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256132.1

ORF Insert Sequence:

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipa2 ( NM_001256130.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243059.1 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05097
Clone ID Related Accession (Same CDS sequence)	XM_006541324.3 , XM_006541324.4 , NM_001256132.1 , XM_006541324.2 , NM_001256130.1 , NM_001256131.1 , NM_023647.6
Accession Version	NM_001256130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	magnesium transporter NIPA2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK041427.1, BY144304.1, AB041581.1, BK001121.1 and AI119710.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-4 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK041427.1, BK001121.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243059.1
CDS	506..1585
Translation	MSLGRGKYDFYIGLGLAMTSSIFIGGSFILKKKGLLRLARKGSMRAGQGGHAYLKEWLWWAGLLSMGAGEVANFAAYAFAPATLVTPLGALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATFVVIVALIFIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELLAGKPVLQHPLAWILLFSLVVCVSTQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAFKDVSFSLASLPVSFRKDEKAMNGNLSSMYEVLNNNEDDLPCGIEHTGENISRRNGNLPSF

Target ORF information:

RefSeq Version	NM_001256130.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256130.1

ORF Insert Sequence:

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipa2 ( NM_001256131.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243060.1 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu05097
Clone ID Related Accession (Same CDS sequence)	XM_006541324.3 , XM_006541324.4 , NM_001256132.1 , XM_006541324.2 , NM_001256130.1 , NM_001256131.1 , NM_023647.6
Accession Version	NM_001256131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	magnesium transporter NIPA2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK041427.1, BY144304.1, AK028734.1, BK001121.1 and AI119710.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-4 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK011248.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243060.1
CDS	385..1464
Translation	MSLGRGKYDFYIGLGLAMTSSIFIGGSFILKKKGLLRLARKGSMRAGQGGHAYLKEWLWWAGLLSMGAGEVANFAAYAFAPATLVTPLGALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATFVVIVALIFIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELLAGKPVLQHPLAWILLFSLVVCVSTQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAFKDVSFSLASLPVSFRKDEKAMNGNLSSMYEVLNNNEDDLPCGIEHTGENISRRNGNLPSF

Target ORF information:

RefSeq Version	NM_001256131.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256131.1

ORF Insert Sequence:

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipa2 ( NM_023647.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076136.2 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05097
Clone ID Related Accession (Same CDS sequence)	XM_006541324.3 , XM_006541324.4 , NM_001256132.1 , XM_006541324.2 , NM_001256130.1 , NM_001256131.1 , NM_023647.6
Accession Version	NM_023647.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	magnesium transporter NIPA2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK041427.1, AK028734.1, BK001121.1 and AI119710.1. On Jan 7, 2012 this sequence version replaced NM_023647.5. Transcript Variant: This variant (1) is the longest transcript. Variants 1-4 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK028734.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076136.2
CDS	1260..2339
Translation	MSLGRGKYDFYIGLGLAMTSSIFIGGSFILKKKGLLRLARKGSMRAGQGGHAYLKEWLWWAGLLSMGAGEVANFAAYAFAPATLVTPLGALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATFVVIVALIFIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELLAGKPVLQHPLAWILLFSLVVCVSTQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAFKDVSFSLASLPVSFRKDEKAMNGNLSSMYEVLNNNEDDLPCGIEHTGENISRRNGNLPSF

Target ORF information:

RefSeq Version	NM_023647.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023647.6

ORF Insert Sequence:

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipa2 ( XM_006541324.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541387.1 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant X1, mRNA.

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CloneID	OMu05097
Clone ID Related Accession (Same CDS sequence)	XM_006541324.3 , XM_006541324.4 , NM_001256132.1 , XM_006541324.2 , NM_001256130.1 , NM_001256131.1 , NM_023647.6
Accession Version	XM_006541324.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	magnesium transporter NIPA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006541324.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541387.1
CDS	578..1657
Translation	MSLGRGKYDFYIGLGLAMTSSIFIGGSFILKKKGLLRLARKGSMRAGQGGHAYLKEWLWWAGLLSMGAGEVANFAAYAFAPATLVTPLGALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATFVVIVALIFIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELLAGKPVLQHPLAWILLFSLVVCVSTQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAFKDVSFSLASLPVSFRKDEKAMNGNLSSMYEVLNNNEDDLPCGIEHTGENISRRNGNLPSF

Target ORF information:

RefSeq Version	XM_006541324.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541324.3

ORF Insert Sequence:

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipa2 ( XM_006541324.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541387.1 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05097
Clone ID Related Accession (Same CDS sequence)	XM_006541324.3 , XM_006541324.4 , NM_001256132.1 , XM_006541324.2 , NM_001256130.1 , NM_001256131.1 , NM_023647.6
Accession Version	XM_006541324.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	magnesium transporter NIPA2 isoform X1
Comment

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541387.1
Translation	MSLGRGKYDFYIGLGLAMTSSIFIGGSFILKKKGLLRLARKGSMRAGQGGHAYLKEWLWWAGLLSMGAGEVANFAAYAFAPATLVTPLGALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATFVVIVALIFIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELLAGKPVLQHPLAWILLFSLVVCVSTQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAFKDVSFSLASLPVSFRKDEKAMNGNLSSMYEVLNNNEDDLPCGIEHTGENISRRNGNLPSF

Target ORF information:

RefSeq Version	XM_006541324.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541324.2

ORF Insert Sequence:

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipa2 ( NM_001256133.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243062.1 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu06046
Clone ID Related Accession (Same CDS sequence)	NM_001256133.1
Accession Version	NM_001256133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	magnesium transporter NIPA2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK041427.1, AK008106.1, AV495155.1, BK001121.1 and AI119710.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream AUG start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## CDS exon combination :: SRR1660819.154380.1, SRR1660817.285842.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGCCG GTGAAGTGGC CAATTTTGCT GCATATGCAT TTGCTCCGGC CACATTAGTG 
ACTCCACTAG GTGCGCTCAG CGTCCTCGTA AGTGCCATTC TGTCTTCATA CTTCCTAAAT 
GAAAGACTTA ATCTTCATGG GAAAATTGGG TGTTTGCTAA GTATTCTAGG GTCTACAGTT 
ATGGTCATTC ATGCTCCAAA GGAAGAGGAG ATTGAGACCT TAAATGAAAT GTCACATAAG 
TTAGGTGATC CAGGTTTTGT GGTCTTTGCA ACATTTGTGG TCATTGTGGC CTTGATATTC 
ATCTTTGTGG TGGGACCTCG ACATGGACAG ACAAACATTC TTGTGTATAT AACAATCTGC 
TCTGTGATTG GAGCATTTTC AGTCTCCTGT GTGAAGGGTT TGGGTATCGC CATAAAAGAG 
TTGTTGGCTG GAAAGCCTGT TCTGCAGCAC CCCCTCGCCT GGATTCTTCT GTTTAGCCTT 
GTGGTCTGTG TGAGCACACA GATTAATTAC CTAAACCGGG CTCTGGACAT TTTCAACACT 
TCCATTGTGA CTCCAATATA TTATGTGTTC TTTACAACTT CAGTTTTGAC TTGTTCAGCT 
ATTCTTTTTA AAGAATGGCA GGATATGCCT GTTGATGATG TCATTGGGAC TTTGAGTGGC 
TTCTTCACAA TCATTGTGGG GATATTTTTG TTGCATGCCT TTAAAGATGT CAGTTTTAGT 
CTAGCAAGTC TGCCAGTATC TTTTCGAAAA GATGAAAAGG CAATGAATGG CAATCTTTCT 
AGTATGTATG AAGTTCTTAA TAATAATGAA GACGACTTAC CCTGTGGAAT TGAACACACT 
GGTGAAAATA TTTCCCGTAG GAATGGAAAT CTGCCATCTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243062.1
CDS	155..1039
Translation	MGAGEVANFAAYAFAPATLVTPLGALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATFVVIVALIFIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELLAGKPVLQHPLAWILLFSLVVCVSTQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAFKDVSFSLASLPVSFRKDEKAMNGNLSSMYEVLNNNEDDLPCGIEHTGENISRRNGNLPSF

Target ORF information:

RefSeq Version	NM_001256133.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256133.1

ORF Insert Sequence:

ATGGGAGCCG GTGAAGTGGC CAATTTTGCT GCATATGCAT TTGCTCCGGC CACATTAGTG 
ACTCCACTAG GTGCGCTCAG CGTCCTCGTA AGTGCCATTC TGTCTTCATA CTTCCTAAAT 
GAAAGACTTA ATCTTCATGG GAAAATTGGG TGTTTGCTAA GTATTCTAGG GTCTACAGTT 
ATGGTCATTC ATGCTCCAAA GGAAGAGGAG ATTGAGACCT TAAATGAAAT GTCACATAAG 
TTAGGTGATC CAGGTTTTGT GGTCTTTGCA ACATTTGTGG TCATTGTGGC CTTGATATTC 
ATCTTTGTGG TGGGACCTCG ACATGGACAG ACAAACATTC TTGTGTATAT AACAATCTGC 
TCTGTGATTG GAGCATTTTC AGTCTCCTGT GTGAAGGGTT TGGGTATCGC CATAAAAGAG 
TTGTTGGCTG GAAAGCCTGT TCTGCAGCAC CCCCTCGCCT GGATTCTTCT GTTTAGCCTT 
GTGGTCTGTG TGAGCACACA GATTAATTAC CTAAACCGGG CTCTGGACAT TTTCAACACT 
TCCATTGTGA CTCCAATATA TTATGTGTTC TTTACAACTT CAGTTTTGAC TTGTTCAGCT 
ATTCTTTTTA AAGAATGGCA GGATATGCCT GTTGATGATG TCATTGGGAC TTTGAGTGGC 
TTCTTCACAA TCATTGTGGG GATATTTTTG TTGCATGCCT TTAAAGATGT CAGTTTTAGT 
CTAGCAAGTC TGCCAGTATC TTTTCGAAAA GATGAAAAGG CAATGAATGG CAATCTTTCT 
AGTATGTATG AAGTTCTTAA TAATAATGAA GACGACTTAC CCTGTGGAAT TGAACACACT 
GGTGAAAATA TTTCCCGTAG GAATGGAAAT CTGCCATCTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipa2 ( XM_006541324.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541387.1 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05097
Clone ID Related Accession (Same CDS sequence)	XM_006541324.3 , XM_006541324.4 , NM_001256132.1 , XM_006541324.2 , NM_001256130.1 , NM_001256131.1 , NM_023647.6
Accession Version	XM_006541324.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	magnesium transporter NIPA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006541324.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541387.1
CDS	585..1664
Translation	MSLGRGKYDFYIGLGLAMTSSIFIGGSFILKKKGLLRLARKGSMRAGQGGHAYLKEWLWWAGLLSMGAGEVANFAAYAFAPATLVTPLGALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATFVVIVALIFIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELLAGKPVLQHPLAWILLFSLVVCVSTQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAFKDVSFSLASLPVSFRKDEKAMNGNLSSMYEVLNNNEDDLPCGIEHTGENISRRNGNLPSF

Target ORF information:

RefSeq Version	XM_006541324.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) (Nipa2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541324.4

ORF Insert Sequence:

ATGAGCCTGG GGCGTGGAAA ATATGATTTT TATATTGGTC TGGGATTGGC TATGACCTCC 
AGCATTTTCA TTGGAGGGAG TTTCATTTTG AAAAAAAAAG GCCTTCTGCG ACTTGCCAGG 
AAAGGCTCAA TGAGAGCAGG TCAAGGAGGC CATGCATATC TTAAGGAATG GTTGTGGTGG 
GCTGGACTGC TGTCAATGGG AGCCGGTGAA GTGGCCAATT TTGCTGCATA TGCATTTGCT 
CCGGCCACAT TAGTGACTCC ACTAGGTGCG CTCAGCGTCC TCGTAAGTGC CATTCTGTCT 
TCATACTTCC TAAATGAAAG ACTTAATCTT CATGGGAAAA TTGGGTGTTT GCTAAGTATT 
CTAGGGTCTA CAGTTATGGT CATTCATGCT CCAAAGGAAG AGGAGATTGA GACCTTAAAT 
GAAATGTCAC ATAAGTTAGG TGATCCAGGT TTTGTGGTCT TTGCAACATT TGTGGTCATT 
GTGGCCTTGA TATTCATCTT TGTGGTGGGA CCTCGACATG GACAGACAAA CATTCTTGTG 
TATATAACAA TCTGCTCTGT GATTGGAGCA TTTTCAGTCT CCTGTGTGAA GGGTTTGGGT 
ATCGCCATAA AAGAGTTGTT GGCTGGAAAG CCTGTTCTGC AGCACCCCCT CGCCTGGATT 
CTTCTGTTTA GCCTTGTGGT CTGTGTGAGC ACACAGATTA ATTACCTAAA CCGGGCTCTG 
GACATTTTCA ACACTTCCAT TGTGACTCCA ATATATTATG TGTTCTTTAC AACTTCAGTT 
TTGACTTGTT CAGCTATTCT TTTTAAAGAA TGGCAGGATA TGCCTGTTGA TGATGTCATT 
GGGACTTTGA GTGGCTTCTT CACAATCATT GTGGGGATAT TTTTGTTGCA TGCCTTTAAA 
GATGTCAGTT TTAGTCTAGC AAGTCTGCCA GTATCTTTTC GAAAAGATGA AAAGGCAATG 
AATGGCAATC TTTCTAGTAT GTATGAAGTT CTTAATAATA ATGAAGACGA CTTACCCTGT 
GGAATTGAAC ACACTGGTGA AAATATTTCC CGTAGGAATG GAAATCTGCC ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional characterization of NIPA2, a selective Mg2+ transporter.
American journal of physiology. Cell physiology295(4)C944-53(2008 Oct)
Goytain A,Hines RM,Quamme GA

Identification of four highly conserved genes between breakpoint hotspots BP1 and BP2 of the Prader-Willi/Angelman syndromes deletion region that have undergone evolutionary transposition mediated by flanking duplicons.
American journal of human genetics73(4)898-925(2003 Oct)
Chai JH,Locke DP,Greally JM,Knoll JH,Ohta T,Dunai J,Yavor A,Eichler EE,Nicholls RD

Protein-protein interaction panel using mouse full-length cDNAs.
Genome research11(10)1758-65(2001 Oct)
Suzuki H,Fukunishi Y,Kagawa I,Saito R,Oda H,Endo T,Kondo S,Bono H,Okazaki Y,Hayashizaki Y

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Nipa2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

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