Trem2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trem2
Entrez Gene ID	83433
Full Name	triggering receptor expressed on myeloid cells 2
Synonyms	TREM-2,Trem2a,Trem2b,Trem2c
General protein information	Preferred Names triggering receptor expressed on myeloid cells 2 Names triggering receptor expressed on myeloid cells 2 triggering receptor expressed on monocytes 2 triggering receptor expressed on myeloid cells 2a triggering receptor expressed on myeloid cells 2b triggering receptor expressed on myeloid cells 2c
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 C
Summary	The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272078.1	NP_001259007.1	triggering receptor expressed on myeloid cells 2 isoform 2
NM_031254.3	NP_112544.1	triggering receptor expressed on myeloid cells 2 isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TREM2	XP_001174118.2
Canis lupus familiaris (dog)	TREM2	XP_005627370.1
Gallus gallus (chicken)	TREM2	NP_001032921.1
Homo sapiens (human)	TREM2	NP_061838.1
	TREM2	XP_001117305.2
Xenopus tropicalis (tropical clawed frog)	trem2	NP_001103522.1
Bos taurus (cattle)	TREM2	NP_001073048.1
Mus musculus (house mouse)	Trem2	NP_112544.1

Gene Ontology
Bibliography

Related articles in PubMed

Bacterial sensor triggering receptor expressed on myeloid cells-2 regulates the mucosal inflammatory response.
Correale C, Genua M, Vetrano S, Mazzini E, Martinoli C, Spinelli A, Arena V, Peyrin-Biroulet L, Caprioli F, Passini N, Panina-Bordignon P, Repici A, Malesci A, Rutella S, Rescigno M, Danese S
Gastroenterology144(2)346-356.e3(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Microglia in Alzheimer's disease (AD) patients carrying TREM2 risk variants and TREM2-deficient mice with AD-like pathology have abundant autophagic vesicles, as do TREM2-deficient macrophages under growth-factor limitation or endoplasmic reticulum (ER) stress. Study concludes that TREM2 enables microglial responses during AD by sustaining cellular energetic and biosynthetic metabolism.
Title: TREM2 Maintains Microglial Metabolic Fitness in Alzheimer's Disease.

The authors demonstrate that a TREM2 loss-of-function mutation causes brain-wide metabolic alterations pointing toward a possible function of microglia in regulating brain glucose metabolism.
Title: The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism.

Triggering receptor expressed on myeloid cells 2 (TREM2) is an immunoglobulin-like receptor of the TREM family and is expressed on activated macrophages, immature dendritic cells, osteoclasts, and microglia.
Title: Triggering Receptor Expressed on Myeloid Cells 2, a Novel Regulator of Immunocyte Phenotypes, Confers Neuroprotection by Relieving Neuroinflammation.

flow cytometry analyses indicated significantly lower surface expression of T66M TREM2 variant than wild type or other TREM2 variants
Title: A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells.

sTREM2 plays a crucial role in regulating microglial cell survival and inflammatory responses.
Title: Soluble TREM2 induces inflammatory responses and enhances microglial survival.

The following Trem2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trem2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17696	NM_001272078.1 Latest version!	Mus musculus triggering receptor expressed on myeloid cells 2 (Trem2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu23273	NM_031254.3 Latest version!	Mus musculus triggering receptor expressed on myeloid cells 2 (Trem2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trem2 ( NM_001272078.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001259007.1 Mus musculus triggering receptor expressed on myeloid cells 2 (Trem2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17696
Clone ID Related Accession (Same CDS sequence)	NM_001272078.1
Accession Version	NM_001272078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Mus musculus(house mouse)
Product	triggering receptor expressed on myeloid cells 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC166164.2, AY187009.1, AK039477.1 and BY541740.1. Transcript Variant: This variant (2) uses an alternate acceptor splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a longer and distinct C-terminus that lacks a transmembrane domain, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155163.1, AY187009.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGACCTC TCCACCAGTT TCTCCTGCTG CTGATCACAG CCCTGTCCCA AGCCCTCAAC 
ACCACGGTGC TGCAGGGCAT GGCCGGCCAG TCCTTGAGGG TGTCATGTAC TTATGACGCC 
TTGAAGCACT GGGGGAGACG CAAGGCCTGG TGTCGGCAGC TGGGTGAGGA GGGCCCATGC 
CAGCGTGTGG TGAGCACACA CGGTGTGTGG CTGCTGGCCT TCCTGAAGAA GCGGAATGGG 
AGCACAGTCA TCGCAGATGA CACCCTTGCT GGAACCGTCA CCATCACTCT GAAGAACCTC 
CAAGCCGGTG ACGCGGGCCT CTACCAGTGT CAGAGTCTCC GAGGCCGAGA GGCTGAGGTC 
CTGCAGAAAG TACTGGTGGA GGTGCTGGAG GACCCTCTAG ATGACCAAGA TGCTGGAGAT 
CTCTGGGTCC CCGAGGAGTC ATCGAGTTTC GAGGGTGCCC AAGTGGAACA CAGCACCTCC 
AGGCAGGTTT CATCCTGTGG GTCACCTCTA GCCTACCACC TTCCTCCTCT TTCCAAGGAA 
TCAAGAGACC TCCTTCCCAC CCACCTCCAT TCTTCTCCTC CTGGCCTGCG TTCTCCTGAG 
CAAGTTTCTT GCAGCCAGCA TCCTCTGGGC TGTGGCCAGG GGCAGGCAGA AGCCGGGAAC 
ACCTGTGGTC AGAGGGCTGG ACTGTGGCCA AGATGCTGGG CACCAACTTC AGATCCTCAC 
TGGACCCGGA GGTACGTGAG AGAATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259007.1
CDS	156..905
Translation	MGPLHQFLLLLITALSQALNTTVLQGMAGQSLRVSCTYDALKHWGRRKAWCRQLGEEGPCQRVVSTHGVWLLAFLKKRNGSTVIADDTLAGTVTITLKNLQAGDAGLYQCQSLRGREAEVLQKVLVEVLEDPLDDQDAGDLWVPEESSSFEGAQVEHSTSRQVSSCGSPLAYHLPPLSKESRDLLPTHLHSSPPGLRSPEQVSCSQHPLGCGQGQAEAGNTCGQRAGLWPRCWAPTSDPHWTRRYVREF

Target ORF information:

RefSeq Version	NM_001272078.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus triggering receptor expressed on myeloid cells 2 (Trem2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272078.1

ORF Insert Sequence:

ATGGGACCTC TCCACCAGTT TCTCCTGCTG CTGATCACAG CCCTGTCCCA AGCCCTCAAC 
ACCACGGTGC TGCAGGGCAT GGCCGGCCAG TCCTTGAGGG TGTCATGTAC TTATGACGCC 
TTGAAGCACT GGGGGAGACG CAAGGCCTGG TGTCGGCAGC TGGGTGAGGA GGGCCCATGC 
CAGCGTGTGG TGAGCACACA CGGTGTGTGG CTGCTGGCCT TCCTGAAGAA GCGGAATGGG 
AGCACAGTCA TCGCAGATGA CACCCTTGCT GGAACCGTCA CCATCACTCT GAAGAACCTC 
CAAGCCGGTG ACGCGGGCCT CTACCAGTGT CAGAGTCTCC GAGGCCGAGA GGCTGAGGTC 
CTGCAGAAAG TACTGGTGGA GGTGCTGGAG GACCCTCTAG ATGACCAAGA TGCTGGAGAT 
CTCTGGGTCC CCGAGGAGTC ATCGAGTTTC GAGGGTGCCC AAGTGGAACA CAGCACCTCC 
AGGCAGGTTT CATCCTGTGG GTCACCTCTA GCCTACCACC TTCCTCCTCT TTCCAAGGAA 
TCAAGAGACC TCCTTCCCAC CCACCTCCAT TCTTCTCCTC CTGGCCTGCG TTCTCCTGAG 
CAAGTTTCTT GCAGCCAGCA TCCTCTGGGC TGTGGCCAGG GGCAGGCAGA AGCCGGGAAC 
ACCTGTGGTC AGAGGGCTGG ACTGTGGCCA AGATGCTGGG CACCAACTTC AGATCCTCAC 
TGGACCCGGA GGTACGTGAG AGAATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trem2 ( NM_031254.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_112544.1 Mus musculus triggering receptor expressed on myeloid cells 2 (Trem2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23273
Clone ID Related Accession (Same CDS sequence)	NM_031254.3
Accession Version	NM_031254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Mus musculus(house mouse)
Product	triggering receptor expressed on myeloid cells 2 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC166164.2, AK039477.1 and BY541740.1. On Jan 10, 2013 this sequence version replaced NM_031254.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052784.1, AY024348.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGACCTC TCCACCAGTT TCTCCTGCTG CTGATCACAG CCCTGTCCCA AGCCCTCAAC 
ACCACGGTGC TGCAGGGCAT GGCCGGCCAG TCCTTGAGGG TGTCATGTAC TTATGACGCC 
TTGAAGCACT GGGGGAGACG CAAGGCCTGG TGTCGGCAGC TGGGTGAGGA GGGCCCATGC 
CAGCGTGTGG TGAGCACACA CGGTGTGTGG CTGCTGGCCT TCCTGAAGAA GCGGAATGGG 
AGCACAGTCA TCGCAGATGA CACCCTTGCT GGAACCGTCA CCATCACTCT GAAGAACCTC 
CAAGCCGGTG ACGCGGGCCT CTACCAGTGT CAGAGTCTCC GAGGCCGAGA GGCTGAGGTC 
CTGCAGAAAG TACTGGTGGA GGTGCTGGAG GACCCTCTAG ATGACCAAGA TGCTGGAGAT 
CTCTGGGTCC CCGAGGAGTC ATCGAGTTTC GAGGGTGCCC AAGTGGAACA CAGCACCTCC 
AGGAATCAAG AGACCTCCTT CCCACCCACC TCCATTCTTC TCCTCCTGGC CTGCGTTCTC 
CTGAGCAAGT TTCTTGCAGC CAGCATCCTC TGGGCTGTGG CCAGGGGCAG GCAGAAGCCG 
GGAACACCTG TGGTCAGAGG GCTGGACTGT GGCCAAGATG CTGGGCACCA ACTTCAGATC 
CTCACTGGAC CCGGAGGTAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112544.1
CDS	156..839
Translation	MGPLHQFLLLLITALSQALNTTVLQGMAGQSLRVSCTYDALKHWGRRKAWCRQLGEEGPCQRVVSTHGVWLLAFLKKRNGSTVIADDTLAGTVTITLKNLQAGDAGLYQCQSLRGREAEVLQKVLVEVLEDPLDDQDAGDLWVPEESSSFEGAQVEHSTSRNQETSFPPTSILLLLACVLLSKFLAASILWAVARGRQKPGTPVVRGLDCGQDAGHQLQILTGPGGT

Target ORF information:

RefSeq Version	NM_031254.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus triggering receptor expressed on myeloid cells 2 (Trem2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031254.3

ORF Insert Sequence:

ATGGGACCTC TCCACCAGTT TCTCCTGCTG CTGATCACAG CCCTGTCCCA AGCCCTCAAC 
ACCACGGTGC TGCAGGGCAT GGCCGGCCAG TCCTTGAGGG TGTCATGTAC TTATGACGCC 
TTGAAGCACT GGGGGAGACG CAAGGCCTGG TGTCGGCAGC TGGGTGAGGA GGGCCCATGC 
CAGCGTGTGG TGAGCACACA CGGTGTGTGG CTGCTGGCCT TCCTGAAGAA GCGGAATGGG 
AGCACAGTCA TCGCAGATGA CACCCTTGCT GGAACCGTCA CCATCACTCT GAAGAACCTC 
CAAGCCGGTG ACGCGGGCCT CTACCAGTGT CAGAGTCTCC GAGGCCGAGA GGCTGAGGTC 
CTGCAGAAAG TACTGGTGGA GGTGCTGGAG GACCCTCTAG ATGACCAAGA TGCTGGAGAT 
CTCTGGGTCC CCGAGGAGTC ATCGAGTTTC GAGGGTGCCC AAGTGGAACA CAGCACCTCC 
AGGAATCAAG AGACCTCCTT CCCACCCACC TCCATTCTTC TCCTCCTGGC CTGCGTTCTC 
CTGAGCAAGT TTCTTGCAGC CAGCATCCTC TGGGCTGTGG CCAGGGGCAG GCAGAAGCCG 
GGAACACCTG TGGTCAGAGG GCTGGACTGT GGCCAAGATG CTGGGCACCA ACTTCAGATC 
CTCACTGGAC CCGGAGGTAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bacterial sensor triggering receptor expressed on myeloid cells-2 regulates the mucosal inflammatory response.
Gastroenterology144(2)346-356.e3(2013 Feb)
Correale C,Genua M,Vetrano S,Mazzini E,Martinoli C,Spinelli A,Arena V,Peyrin-Biroulet L,Caprioli F,Passini N,Panina-Bordignon P,Repici A,Malesci A,Rutella S,Rescigno M,Danese S

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Trem2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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