Srd5a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srd5a1
Entrez Gene ID	78925
Full Name	steroid 5 alpha-reductase 1
Synonyms	0610031P22Rik,4930435F02Rik,S5AR 1,Srd5a-1
General protein information	Preferred Names steroid 5 alpha-reductase 1 Names 3-oxo-5-alpha-steroid 4-dehydrogenase 1 SR type 1 type 1 5alpha-reductase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 B3-C1\|13 35.55 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006517473.3	XP_006517536.1	3-oxo-5-alpha-steroid 4-dehydrogenase 1 isoform X1
NM_175283.3	NP_780492.2	3-oxo-5-alpha-steroid 4-dehydrogenase 1
XM_006517473.4	XP_006517536.1	3-oxo-5-alpha-steroid 4-dehydrogenase 1 isoform X1
XM_006517474.3	XP_006517537.1	3-oxo-5-alpha-steroid 4-dehydrogenase 1 isoform X2
XM_006517474.4	XP_006517537.1	3-oxo-5-alpha-steroid 4-dehydrogenase 1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SRD5A1	NP_001038.1
	SRD5A1	XP_001083405.1
Mus musculus (house mouse)	Srd5a1	NP_780492.2
Schizosaccharomyces pombe (fission yeast)	SPAC9.08c	NP_593351.1
Oryza sativa (rice)	Os11g0184100	NP_001176391.1
Canis lupus familiaris (dog)	SRD5A1	XP_535799.2
Gallus gallus (chicken)	SRD5A1	XP_004935188.1
Xenopus tropicalis (tropical clawed frog)	srd5a1	NP_001006841.1
Pan troglodytes (chimpanzee)	SRD5A1	XP_001144570.1
Rattus norvegicus (Norway rat)	Srd5a1	NP_058766.2
Arabidopsis thaliana (thale cress)	DET2	NP_181340.1
Oryza sativa (rice)	Os01g0851600	NP_001044822.1
Bos taurus (cattle)	SRD5A1	NP_001092607.1
Danio rerio (zebrafish)	srd5a1	NP_001070121.1
Magnaporthe oryzae (rice blast fungus)	MGG_03905	XP_003719969.1

Gene Ontology
Bibliography

Related articles in PubMed

Metabolic dysfunction in female mice with disruption of 5?-reductase 1.
Livingstone DE, Di Rollo EM, Mak TC, Sooy K, Walker BR, Andrew R
The Journal of endocrinology232(1)29-36(2017 Jan)

5?-reductase 1 mRNA levels are positively correlated with TRAMP mouse prostate most severe lesion scores.
Opoku-Acheampong AB, Henningson JN, Beck AP, Lindshield BL
PloS one12(5)e0175874(2017)

Sex chromosome complement determines sex differences in aromatase expression and regulation in the stria terminalis and anterior amygdala of the developing mouse brain.
Cisternas CD, Tome K, Caeiro XE, Dadam FM, Garcia-Segura LM, Cambiasso MJ
Molecular and cellular endocrinology41499-110(2015 Oct)

DNA methylation of the GC box in the promoter region mediates isolation rearing-induced suppression of srd5a1 transcription in the prefrontal cortex.
Araki R, Nishida S, Hiraki Y, Matsumoto K, Yabe T
Neuroscience letters606135-9(2015 Oct)

The role of adrenal gland microenvironment in the HPA axis function and dysfunction during sepsis.
Kanczkowski W, Sue M, Zacharowski K, Reincke M, Bornstein SR
Molecular and cellular endocrinology408241-8(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Exaggerated predisposition to metabolic disorders in female mice, compared with that seen in male mice, after disruption of 5alphaR1 suggests phenotypic changes may be underpinned by altered metabolism of glucocorticoids rather than androgens.
Title: Metabolic dysfunction in female mice with disruption of 5?-reductase 1.

Prostate 5aR1 levels were positively correlated with adjusted prostate most severe lesion scores. Downregulation of androgen receptor and 5alpha-reductase 2, along with upregulation of 5a-reductase 1 in tumors may promote prostatic intraepithelial neoplasia and prostate cancer development in TRAMP mice
Title: 5?-reductase 1 mRNA levels are positively correlated with TRAMP mouse prostate most severe lesion scores.

transcription of srd5a1 in brain is regulated by environmental factor-induced cytosine methylation in the promoter region.
Title: DNA methylation of the GC box in the promoter region mediates isolation rearing-induced suppression of srd5a1 transcription in the prefrontal cortex.

the Srd5a1 gene modulates ethanol consumption in a sex-dependent manner that is also contingent upon ethanol access condition and concentration
Title: Null mutation of 5?-reductase type I gene alters ethanol consumption patterns in a sex-dependent manner.

deletion of the Srd5a1 gene significantly decreased ethanol's anxiolytic effect in female mice, and chronic ethanol withdrawal severity was lower in female versus male mice
Title: Sex Differences in Ethanol's Anxiolytic Effect and Chronic Ethanol Withdrawal Severity in Mice with a Null Mutation of the 5?-Reductase Type 1 Gene.

The following Srd5a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srd5a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17983	NM_175283.3 Latest version!	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu48919	XM_006517473.4 Latest version!	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu48920	XM_006517474.4 Latest version!	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu48919	XM_006517473.3	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu48920	XM_006517474.3	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srd5a1 ( XM_006517473.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517536.1 Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48919
Clone ID Related Accession (Same CDS sequence)	XM_006517473.3 , XM_006517473.4
Accession Version	XM_006517473.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	3-oxo-5-alpha-steroid 4-dehydrogenase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517473.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG CTCTGCAAAT TCACAGCCTC ACCGGGCAGC ATTTCCTACT GACTGAAAGT 
TTACATCGTG AACCACAGTT AGATCTGGCT TTTTGGAAGA TGATTAAGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517536.1
CDS	661..1314
Translation	MELDELRLLDALVYLEGFLAFVAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLLAMFLIHYVQRTLVFPVLIRGGKPTLLFTFVLAFLFCTLNGYLQSRYLSQFAVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRALQIHSLTGQHFLLTESLHREPQLDLAFWKMIKG

Target ORF information:

RefSeq Version	XM_006517473.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517473.3

ORF Insert Sequence:

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG CTCTGCAAAT TCACAGCCTC ACCGGGCAGC ATTTCCTACT GACTGAAAGT 
TTACATCGTG AACCACAGTT AGATCTGGCT TTTTGGAAGA TGATTAAGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srd5a1 ( NM_175283.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780492.2 Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), mRNA.

Price & Availability↑

CloneID	OMu17983
Clone ID Related Accession (Same CDS sequence)	NM_175283.3
Accession Version	NM_175283.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	3-oxo-5-alpha-steroid 4-dehydrogenase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB585840.1 and BC079863.1. On Feb 8, 2006 this sequence version replaced NM_175283.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC079863.1, BC094503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG GAGGCCTGTT TGAATATGTA TCTTCAGCCA ACTATTTTGG GGAGCTCGTG 
GAGTGGTGTG GCTTTGCACT GGCCAGCTGG TCCCTCCAGG GCGTGGTGTT TGCTCTGTTC 
ACCCTGTGTG CACTGTTCAC CAGAGCGAGG CAGCATCATC AGTGGTACCT CGAGAAGTTT 
GAAGATTACC CCAAAACAAG AAAAATACTA ATTCCATTCC TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780492.2
CDS	217..984
Translation	MELDELRLLDALVYLEGFLAFVAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLLAMFLIHYVQRTLVFPVLIRGGKPTLLFTFVLAFLFCTLNGYLQSRYLSQFAVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKTRKILIPFLL

Target ORF information:

RefSeq Version	NM_175283.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175283.3

ORF Insert Sequence:

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG GAGGCCTGTT TGAATATGTA TCTTCAGCCA ACTATTTTGG GGAGCTCGTG 
GAGTGGTGTG GCTTTGCACT GGCCAGCTGG TCCCTCCAGG GCGTGGTGTT TGCTCTGTTC 
ACCCTGTGTG CACTGTTCAC CAGAGCGAGG CAGCATCATC AGTGGTACCT CGAGAAGTTT 
GAAGATTACC CCAAAACAAG AAAAATACTA ATTCCATTCC TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srd5a1 ( XM_006517473.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517536.1 Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48919
Clone ID Related Accession (Same CDS sequence)	XM_006517473.3 , XM_006517473.4
Accession Version	XM_006517473.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	3-oxo-5-alpha-steroid 4-dehydrogenase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517473.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG CTCTGCAAAT TCACAGCCTC ACCGGGCAGC ATTTCCTACT GACTGAAAGT 
TTACATCGTG AACCACAGTT AGATCTGGCT TTTTGGAAGA TGATTAAGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517536.1
CDS	239..892
Translation	MELDELRLLDALVYLEGFLAFVAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLLAMFLIHYVQRTLVFPVLIRGGKPTLLFTFVLAFLFCTLNGYLQSRYLSQFAVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRALQIHSLTGQHFLLTESLHREPQLDLAFWKMIKG

Target ORF information:

RefSeq Version	XM_006517473.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517473.4

ORF Insert Sequence:

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG CTCTGCAAAT TCACAGCCTC ACCGGGCAGC ATTTCCTACT GACTGAAAGT 
TTACATCGTG AACCACAGTT AGATCTGGCT TTTTGGAAGA TGATTAAGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srd5a1 ( XM_006517474.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517537.1 Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu48920
Clone ID Related Accession (Same CDS sequence)	XM_006517474.3 , XM_006517474.4
Accession Version	XM_006517474.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	3-oxo-5-alpha-steroid 4-dehydrogenase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517474.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG GTAAGCATGG AGGGAAACTG GATACAAAAT ACCCAGGGGT AAGCATGGAG 
GGAAACTGGA TACAAAATAC CCAGGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517537.1
CDS	235..864
Translation	MELDELRLLDALVYLEGFLAFVAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLLAMFLIHYVQRTLVFPVLIRGGKPTLLFTFVLAFLFCTLNGYLQSRYLSQFAVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGKHGGKLDTKYPGVSMEGNWIQNTQG

Target ORF information:

RefSeq Version	XM_006517474.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517474.4

ORF Insert Sequence:

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG GTAAGCATGG AGGGAAACTG GATACAAAAT ACCCAGGGGT AAGCATGGAG 
GGAAACTGGA TACAAAATAC CCAGGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srd5a1 ( XM_006517474.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517537.1 Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X2, mRNA.

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CloneID	OMu48920
Clone ID Related Accession (Same CDS sequence)	XM_006517474.3 , XM_006517474.4
Accession Version	XM_006517474.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	3-oxo-5-alpha-steroid 4-dehydrogenase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517474.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG GTAAGCATGG AGGGAAACTG GATACAAAAT ACCCAGGGGT AAGCATGGAG 
GGAAACTGGA TACAAAATAC CCAGGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517537.1
CDS	661..1290
Translation	MELDELRLLDALVYLEGFLAFVAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLLAMFLIHYVQRTLVFPVLIRGGKPTLLFTFVLAFLFCTLNGYLQSRYLSQFAVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGKHGGKLDTKYPGVSMEGNWIQNTQG

Target ORF information:

RefSeq Version	XM_006517474.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid 5 alpha-reductase 1 (Srd5a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517474.3

ORF Insert Sequence:

ATGGAGTTGG ATGAGTTGCG CCTACTGGAT GCGCTAGTCT ACCTGGAGGG TTTCCTGGCT 
TTCGTGGCCT TCGTGGGGCT CCAGATGGTG GGCTCTTCCT ACGGCCGCTA CTCCTCGCAG 
TGGTCCGGCC GCCGAGTGCC CGCGCGACCT GCCTGGTTCC TACAGGAGCT GCCTTCAATG 
GCCTGGCCGC TGTACGAGTG CATCCGTCCT GCAGCCGCCC GACTGGGCAA CCTGCCTAAC 
CGCGTCCTGC TAGCTATGTT TCTGATCCAC TACGTGCAAA GGACGCTGGT TTTTCCAGTT 
CTGATCAGGG GAGGGAAGCC CACCCTGCTG TTCACCTTTG TCTTGGCCTT CCTGTTCTGC 
ACCTTAAATG GCTACTTGCA GAGCCGATAC TTGAGCCAGT TTGCGGTGTA TGCTGAAGAC 
TGGGTAACCC ATCCCTGTTT CCTGACAGGC TTTGCCCTGT GGTTAGTGGG CATGGTGATA 
AATATCCACT CAGACCACAT CCTGCGGAAT CTGAGAAAAC CAGGGGAAAC TGGATACAAA 
ATACCCAGGG GTAAGCATGG AGGGAAACTG GATACAAAAT ACCCAGGGGT AAGCATGGAG 
GGAAACTGGA TACAAAATAC CCAGGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Metabolic dysfunction in female mice with disruption of 5?-reductase 1.
The Journal of endocrinology232(1)29-36(2017 Jan)
Livingstone DE,Di Rollo EM,Mak TC,Sooy K,Walker BR,Andrew R

5?-reductase 1 mRNA levels are positively correlated with TRAMP mouse prostate most severe lesion scores.
PloS one12(5)e0175874(2017)
Opoku-Acheampong AB,Henningson JN,Beck AP,Lindshield BL

Sex chromosome complement determines sex differences in aromatase expression and regulation in the stria terminalis and anterior amygdala of the developing mouse brain.
Molecular and cellular endocrinology41499-110(2015 Oct)
Cisternas CD,Tome K,Caeiro XE,Dadam FM,Garcia-Segura LM,Cambiasso MJ

DNA methylation of the GC box in the promoter region mediates isolation rearing-induced suppression of srd5a1 transcription in the prefrontal cortex.
Neuroscience letters606135-9(2015 Oct)
Araki R,Nishida S,Hiraki Y,Matsumoto K,Yabe T

The role of adrenal gland microenvironment in the HPA axis function and dysfunction during sepsis.
Molecular and cellular endocrinology408241-8(2015 Jun)
Kanczkowski W,Sue M,Zacharowski K,Reincke M,Bornstein SR

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Srd5a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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