Pde6h cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde6h
Entrez Gene ID	78600
Full Name	phosphodiesterase 6H, cGMP-specific, cone, gamma
Synonyms	A930033D18Rik,PDEgamma
General protein information	Preferred Names phosphodiesterase 6H, cGMP-specific, cone, gamma Names retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma GMP-PDE gamma cone phosphodiesterase 6 gamma cone-like cGMP-phosphodiesterase 6 gamma subunit type 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 G1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006506773.2	XP_006506836.1	retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
NM_023898.4	NP_076387.2	retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
XM_006506774.1	XP_006506837.1	retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
XM_006506773.3	XP_006506836.1	retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PDE6H	XP_001158553.1
Canis lupus familiaris (dog)	PDE6H	XP_005637168.1
Bos taurus (cattle)	PDE6H	NP_776847.1
Rattus norvegicus (Norway rat)	Pde6h	NP_446140.1
	PDE6H	XP_001091111.1
Mus musculus (house mouse)	Pde6h	NP_076387.2
Gallus gallus (chicken)	PDE6H	NP_989739.1
Homo sapiens (human)	PDE6H	NP_006196.1
Danio rerio (zebrafish)	zgc:73359	NP_957131.1
Xenopus tropicalis (tropical clawed frog)	pde6h	NP_001016745.1

Gene Ontology
Bibliography

Related articles in PubMed

Mef2d is essential for the maturation and integrity of retinal photoreceptor and bipolar cells.
Omori Y, Kitamura T, Yoshida S, Kuwahara R, Chaya T, Irie S, Furukawa T
Genes to cells : devoted to molecular & cellular mechanisms20(5)408-26(2015 May)

Targeted ablation of the Pde6h gene in mice reveals cross-species differences in cone and rod phototransduction protein isoform inventory.
Brennenstuhl C, Tanimoto N, Burkard M, Wagner R, Bolz S, Trifunovic D, Kabagema-Bilan C, Paquet-Durand F, Beck SC, Huber G, Seeliger MW, Ruth P, Wissinger B, Lukowski R
The Journal of biological chemistry290(16)10242-55(2015 Apr)

Light-dependent phosphorylation of the gamma subunit of cGMP-phophodiesterase (PDE6gamma) at residue threonine 22 in intact photoreceptor neurons.
Janisch KM, Kasanuki JM, Naumann MC, Davis RJ, Lin CS, Semple-Rowland S, Tsang SH
Biochemical and biophysical research communications390(4)1149-53(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pde6h deficiency mice suggests no defects in cone/rod-driven retinal signaling and therefore preserved visual functions.
Title: Targeted ablation of the Pde6h gene in mice reveals cross-species differences in cone and rod phototransduction protein isoform inventory.

The interdependency of phosphorylation of T22 and T35 suggests that light-induced, post-translational modification of PDE6gamma is essential for the regulation of G-protein signaling.
Title: Light-dependent phosphorylation of the gamma subunit of cGMP-phophodiesterase (PDE6gamma) at residue threonine 22 in intact photoreceptor neurons.

This experiments provide evidence for modulation of Pde6h dependent response turnoff in mouse rod photoreceptors.
Title: Modulation of phosphodiesterase6 turnoff during background illumination in mouse rod photoreceptors.

Our results show that the PDE6gamma binding sites of PDE6 alpha and beta are accessible to excess (presumably cytoplasmic) PDE6gamma in the light, once endogenous PDE6gamma has been displaced from its binding site by Talpha-GTP.
Title: GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase.

The following Pde6h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde6h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07962	NM_023898.4 Latest version!	Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu07962	XM_006506773.3 Latest version!	Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu07962	XM_006506774.1 Latest version!	Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu07962	XM_006506773.2	Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde6h ( NM_023898.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076387.2 Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), mRNA.

Price & Availability↑

CloneID	OMu07962
Clone ID Related Accession (Same CDS sequence)	XM_006506773.2 , NM_023898.4 , XM_006506774.1 , XM_006506773.3
Accession Version	NM_023898.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK020922.1 and BE983779.1. On Sep 8, 2006 this sequence version replaced NM_023898.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY742316.1, AK020922.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGACA GCCCTTCCCT GAGTCCTCCA GCACCAAGCC AGGGCCCTAC TACCCCACGC 
AAAGGGCCCC CCAAGTTCAA GCAGAGGCAG ACTCGACAGT TCAAGAGCAA GCCTCCCAAG 
AAAGGGGTGA AAGGGTTTGG AGATGACATC CCAGGCATGG AGGGGCTAGG AACAGATATC 
ACGGTCATCT GTCCCTGGGA AGCGTTCAGC CATCTGGAGC TGCATGAGCT TGCTCAGTTC 
GGGATCATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076387.2
CDS	93..344
Translation	MSDSPSLSPPAPSQGPTTPRKGPPKFKQRQTRQFKSKPPKKGVKGFGDDIPGMEGLGTDITVICPWEAFSHLELHELAQFGII

Target ORF information:

RefSeq Version	NM_023898.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023898.4

ORF Insert Sequence:

ATGAGTGACA GCCCTTCCCT GAGTCCTCCA GCACCAAGCC AGGGCCCTAC TACCCCACGC 
AAAGGGCCCC CCAAGTTCAA GCAGAGGCAG ACTCGACAGT TCAAGAGCAA GCCTCCCAAG 
AAAGGGGTGA AAGGGTTTGG AGATGACATC CCAGGCATGG AGGGGCTAGG AACAGATATC 
ACGGTCATCT GTCCCTGGGA AGCGTTCAGC CATCTGGAGC TGCATGAGCT TGCTCAGTTC 
GGGATCATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde6h ( XM_006506773.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506836.1 Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu07962
Clone ID Related Accession (Same CDS sequence)	XM_006506773.2 , NM_023898.4 , XM_006506774.1 , XM_006506773.3
Accession Version	XM_006506773.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
Comment

ATGAGTGACA GCCCTTCCCT GAGTCCTCCA GCACCAAGCC AGGGCCCTAC TACCCCACGC 
AAAGGGCCCC CCAAGTTCAA GCAGAGGCAG ACTCGACAGT TCAAGAGCAA GCCTCCCAAG 
AAAGGGGTGA AAGGGTTTGG AGATGACATC CCAGGCATGG AGGGGCTAGG AACAGATATC 
ACGGTCATCT GTCCCTGGGA AGCGTTCAGC CATCTGGAGC TGCATGAGCT TGCTCAGTTC 
GGGATCATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506836.1
Translation	MSDSPSLSPPAPSQGPTTPRKGPPKFKQRQTRQFKSKPPKKGVKGFGDDIPGMEGLGTDITVICPWEAFSHLELHELAQFGII

Target ORF information:

RefSeq Version	XM_006506773.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506773.2

ORF Insert Sequence:

ATGAGTGACA GCCCTTCCCT GAGTCCTCCA GCACCAAGCC AGGGCCCTAC TACCCCACGC 
AAAGGGCCCC CCAAGTTCAA GCAGAGGCAG ACTCGACAGT TCAAGAGCAA GCCTCCCAAG 
AAAGGGGTGA AAGGGTTTGG AGATGACATC CCAGGCATGG AGGGGCTAGG AACAGATATC 
ACGGTCATCT GTCCCTGGGA AGCGTTCAGC CATCTGGAGC TGCATGAGCT TGCTCAGTTC 
GGGATCATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde6h ( XM_006506774.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506837.1 Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu07962
Clone ID Related Accession (Same CDS sequence)	XM_006506773.2 , NM_023898.4 , XM_006506774.1 , XM_006506773.3
Accession Version	XM_006506774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
Comment

ATGAGTGACA GCCCTTCCCT GAGTCCTCCA GCACCAAGCC AGGGCCCTAC TACCCCACGC 
AAAGGGCCCC CCAAGTTCAA GCAGAGGCAG ACTCGACAGT TCAAGAGCAA GCCTCCCAAG 
AAAGGGGTGA AAGGGTTTGG AGATGACATC CCAGGCATGG AGGGGCTAGG AACAGATATC 
ACGGTCATCT GTCCCTGGGA AGCGTTCAGC CATCTGGAGC TGCATGAGCT TGCTCAGTTC 
GGGATCATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506837.1
Translation	MSDSPSLSPPAPSQGPTTPRKGPPKFKQRQTRQFKSKPPKKGVKGFGDDIPGMEGLGTDITVICPWEAFSHLELHELAQFGII

Target ORF information:

RefSeq Version	XM_006506774.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506774.1

ORF Insert Sequence:

ATGAGTGACA GCCCTTCCCT GAGTCCTCCA GCACCAAGCC AGGGCCCTAC TACCCCACGC 
AAAGGGCCCC CCAAGTTCAA GCAGAGGCAG ACTCGACAGT TCAAGAGCAA GCCTCCCAAG 
AAAGGGGTGA AAGGGTTTGG AGATGACATC CCAGGCATGG AGGGGCTAGG AACAGATATC 
ACGGTCATCT GTCCCTGGGA AGCGTTCAGC CATCTGGAGC TGCATGAGCT TGCTCAGTTC 
GGGATCATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde6h ( XM_006506773.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506836.1 Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu07962
Clone ID Related Accession (Same CDS sequence)	XM_006506773.2 , NM_023898.4 , XM_006506774.1 , XM_006506773.3
Accession Version	XM_006506773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACA GCCCTTCCCT GAGTCCTCCA GCACCAAGCC AGGGCCCTAC TACCCCACGC 
AAAGGGCCCC CCAAGTTCAA GCAGAGGCAG ACTCGACAGT TCAAGAGCAA GCCTCCCAAG 
AAAGGGGTGA AAGGGTTTGG AGATGACATC CCAGGCATGG AGGGGCTAGG AACAGATATC 
ACGGTCATCT GTCCCTGGGA AGCGTTCAGC CATCTGGAGC TGCATGAGCT TGCTCAGTTC 
GGGATCATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506836.1
CDS	128..379
Translation	MSDSPSLSPPAPSQGPTTPRKGPPKFKQRQTRQFKSKPPKKGVKGFGDDIPGMEGLGTDITVICPWEAFSHLELHELAQFGII

Target ORF information:

RefSeq Version	XM_006506773.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506773.3

ORF Insert Sequence:

ATGAGTGACA GCCCTTCCCT GAGTCCTCCA GCACCAAGCC AGGGCCCTAC TACCCCACGC 
AAAGGGCCCC CCAAGTTCAA GCAGAGGCAG ACTCGACAGT TCAAGAGCAA GCCTCCCAAG 
AAAGGGGTGA AAGGGTTTGG AGATGACATC CCAGGCATGG AGGGGCTAGG AACAGATATC 
ACGGTCATCT GTCCCTGGGA AGCGTTCAGC CATCTGGAGC TGCATGAGCT TGCTCAGTTC 
GGGATCATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mef2d is essential for the maturation and integrity of retinal photoreceptor and bipolar cells.
Genes to cells : devoted to molecular & cellular mechanisms20(5)408-26(2015 May)
Omori Y,Kitamura T,Yoshida S,Kuwahara R,Chaya T,Irie S,Furukawa T

Targeted ablation of the Pde6h gene in mice reveals cross-species differences in cone and rod phototransduction protein isoform inventory.
The Journal of biological chemistry290(16)10242-55(2015 Apr)
Brennenstuhl C,Tanimoto N,Burkard M,Wagner R,Bolz S,Trifunovic D,Kabagema-Bilan C,Paquet-Durand F,Beck SC,Huber G,Seeliger MW,Ruth P,Wissinger B,Lukowski R

Light-dependent phosphorylation of the gamma subunit of cGMP-phophodiesterase (PDE6gamma) at residue threonine 22 in intact photoreceptor neurons.
Biochemical and biophysical research communications390(4)1149-53(2009 Dec)
Janisch KM,Kasanuki JM,Naumann MC,Davis RJ,Lin CS,Semple-Rowland S,Tsang SH

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Pde6h cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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