Mrap cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrap
Entrez Gene ID	77037
Full Name	melanocortin 2 receptor accessory protein
Synonyms	1110025G12Rik,C21ORF61,Falp,ORF61
General protein information	Preferred Names melanocortin 2 receptor accessory protein Names melanocortin-2 receptor accessory protein fat cell-specific low molecular weight protein fat tissue-specific low MW protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_029844.3	NP_084120.1	melanocortin-2 receptor accessory protein
NM_029844.4	NP_084120.1	melanocortin-2 receptor accessory protein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mrap	NP_001129306.1
	MRAP	XP_001096562.1
Bos taurus (cattle)	MRAP	XP_002684630.1
Homo sapiens (human)	MRAP	NP_848932.1
Pan troglodytes (chimpanzee)	MRAP	NP_001065291.2
Canis lupus familiaris (dog)	MRAP	XP_005638887.1
Mus musculus (house mouse)	Mrap	NP_084120.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of Lipolytic Response and Energy Balance by Melanocortin 2 Receptor Accessory Protein (MRAP) in Adipocytes.
Zhang X, Saarinen AM, Campbell LE, De Filippis EA, Liu J
Diabetes67(2)222-234(2018 Feb)

Transcriptional activation of melanocortin 2 receptor accessory protein by PPAR? in adipocytes.
Kim NS, Kim YJ, Cho SY, Lee TR, Kim SH
Biochemical and biophysical research communications439(3)401-6(2013 Sep)

Functional expression of Squalus acanthias melanocortin-5 receptor in CHO cells: ligand selectivity and interaction with MRAP.
Reinick CL, Liang L, Angleson JK, Dores RM
European journal of pharmacology680(1-3)1-7(2012 Apr)

Use of chimeric melanocortin-2 and -4 receptors to identify regions responsible for ligand specificity and dependence on melanocortin 2 receptor accessory protein.
Hinkle PM, Serasinghe MN, Jakabowski A, Sebag JA, Wilson KR, Haskell-Luevano C
European journal of pharmacology660(1)94-102(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that PPARgamma is required for transcriptional activation of the MRAP gene during adipogenesis, which contributes to understanding of the molecular mechanism of lipolysis in adipocytes.
Title: Transcriptional activation of melanocortin 2 receptor accessory protein by PPAR? in adipocytes.

The receptor was co-expressed with a cartilaginous fish (Callorhinchus milii) MRAP2 cDNA.
Title: Functional expression of Squalus acanthias melanocortin-5 receptor in CHO cells: ligand selectivity and interaction with MRAP.

C-terminal tail of the MC(4) receptor with the corresponding regions from the MC(2) receptor resulted in MRAP-dependent signaling
Title: Use of chimeric melanocortin-2 and -4 receptors to identify regions responsible for ligand specificity and dependence on melanocortin 2 receptor accessory protein.

The results establish that MRAP forms stable complexes with two different melanocortin receptors, facilitating surface expression of MC2 receptor but disrupting dimerization and surface localization of MC5 receptor.
Title: Opposite effects of the melanocortin-2 (MC2) receptor accessory protein MRAP on MC2 and MC5 receptor dimerization and trafficking.

MRAP not only facilitates MC2 receptor trafficking but also allows properly localized receptor to bind ACTH and consequently signal.
Title: Regions of melanocortin 2 (MC2) receptor accessory protein necessary for dual topology and MC2 receptor trafficking and signaling.

The following Mrap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07565	NM_029844.4 Latest version!	Mus musculus melanocortin 2 receptor accessory protein (Mrap), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu07565	NM_029844.3	Mus musculus melanocortin 2 receptor accessory protein (Mrap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrap ( NM_029844.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084120.1 Mus musculus melanocortin 2 receptor accessory protein (Mrap), mRNA.

Price & Availability↑

CloneID	OMu07565
Clone ID Related Accession (Same CDS sequence)	NM_029844.3 , NM_029844.4
Accession Version	NM_029844.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Mus musculus(house mouse)
Product	melanocortin-2 receptor accessory protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC126671.3. On May 1, 2007 this sequence version replaced NM_029844.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK003912.2, BC027543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAACG GGACCGACGC CTCTGTCCCG CTCACCAGCT ATGAGTATTA CCTGGACTAC 
ATAGACCTCA TTCCTGTGGA CGAGAAGAAG CTGAAAGCCA ACAAGCATTC CATTGTCATC 
GCCCTGTGGT TGAGCCTGGC TACCTTCGTG GTGCTCCTCT TTCTCATCCT GCTCTACATG 
TCCTGGTCGG GCTCCCCACA GATGAGGCAC AGTCCCCAAC CCCAGCCAAT ATGTTCATGG 
ACTCACAGCT TCAACCTCCC TCTGTGCCTC CGGAGGGCCT CCCTGCAGAC AACAGAGGAG 
CCAGGAAGGA GAGCTGGCAC TGACCAGTGG TTAACGCAGC AGAGTCCTTC TGCCTCAGCC 
CCGGGGCCCC TGGCTCTCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084120.1
CDS	241..624
Translation	MANGTDASVPLTSYEYYLDYIDLIPVDEKKLKANKHSIVIALWLSLATFVVLLFLILLYMSWSGSPQMRHSPQPQPICSWTHSFNLPLCLRRASLQTTEEPGRRAGTDQWLTQQSPSASAPGPLALP

Target ORF information:

RefSeq Version	NM_029844.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanocortin 2 receptor accessory protein (Mrap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029844.3

ORF Insert Sequence:

ATGGCCAACG GGACCGACGC CTCTGTCCCG CTCACCAGCT ATGAGTATTA CCTGGACTAC 
ATAGACCTCA TTCCTGTGGA CGAGAAGAAG CTGAAAGCCA ACAAGCATTC CATTGTCATC 
GCCCTGTGGT TGAGCCTGGC TACCTTCGTG GTGCTCCTCT TTCTCATCCT GCTCTACATG 
TCCTGGTCGG GCTCCCCACA GATGAGGCAC AGTCCCCAAC CCCAGCCAAT ATGTTCATGG 
ACTCACAGCT TCAACCTCCC TCTGTGCCTC CGGAGGGCCT CCCTGCAGAC AACAGAGGAG 
CCAGGAAGGA GAGCTGGCAC TGACCAGTGG TTAACGCAGC AGAGTCCTTC TGCCTCAGCC 
CCGGGGCCCC TGGCTCTCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrap ( NM_029844.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084120.1 Mus musculus melanocortin 2 receptor accessory protein (Mrap), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07565
Clone ID Related Accession (Same CDS sequence)	NM_029844.3 , NM_029844.4
Accession Version	NM_029844.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	melanocortin-2 receptor accessory protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126671.3. On Apr 20, 2018 this sequence version replaced NM_029844.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027543.1, AK003912.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACG GGACCGACGC CTCTGTCCCG CTCACCAGCT ATGAGTATTA CCTGGACTAC 
ATAGACCTCA TTCCTGTGGA CGAGAAGAAG CTGAAAGCCA ACAAGCATTC CATTGTCATC 
GCCCTGTGGT TGAGCCTGGC TACCTTCGTG GTGCTCCTCT TTCTCATCCT GCTCTACATG 
TCCTGGTCGG GCTCCCCACA GATGAGGCAC AGTCCCCAAC CCCAGCCAAT ATGTTCATGG 
ACTCACAGCT TCAACCTCCC TCTGTGCCTC CGGAGGGCCT CCCTGCAGAC AACAGAGGAG 
CCAGGAAGGA GAGCTGGCAC TGACCAGTGG TTAACGCAGC AGAGTCCTTC TGCCTCAGCC 
CCGGGGCCCC TGGCTCTCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084120.1
CDS	358..741
Translation	MANGTDASVPLTSYEYYLDYIDLIPVDEKKLKANKHSIVIALWLSLATFVVLLFLILLYMSWSGSPQMRHSPQPQPICSWTHSFNLPLCLRRASLQTTEEPGRRAGTDQWLTQQSPSASAPGPLALP

Target ORF information:

RefSeq Version	NM_029844.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanocortin 2 receptor accessory protein (Mrap), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029844.4

ORF Insert Sequence:

ATGGCCAACG GGACCGACGC CTCTGTCCCG CTCACCAGCT ATGAGTATTA CCTGGACTAC 
ATAGACCTCA TTCCTGTGGA CGAGAAGAAG CTGAAAGCCA ACAAGCATTC CATTGTCATC 
GCCCTGTGGT TGAGCCTGGC TACCTTCGTG GTGCTCCTCT TTCTCATCCT GCTCTACATG 
TCCTGGTCGG GCTCCCCACA GATGAGGCAC AGTCCCCAAC CCCAGCCAAT ATGTTCATGG 
ACTCACAGCT TCAACCTCCC TCTGTGCCTC CGGAGGGCCT CCCTGCAGAC AACAGAGGAG 
CCAGGAAGGA GAGCTGGCAC TGACCAGTGG TTAACGCAGC AGAGTCCTTC TGCCTCAGCC 
CCGGGGCCCC TGGCTCTCCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of Lipolytic Response and Energy Balance by Melanocortin 2 Receptor Accessory Protein (MRAP) in Adipocytes.
Diabetes67(2)222-234(2018 Feb)
Zhang X,Saarinen AM,Campbell LE,De Filippis EA,Liu J

Transcriptional activation of melanocortin 2 receptor accessory protein by PPAR? in adipocytes.
Biochemical and biophysical research communications439(3)401-6(2013 Sep)
Kim NS,Kim YJ,Cho SY,Lee TR,Kim SH

Functional expression of Squalus acanthias melanocortin-5 receptor in CHO cells: ligand selectivity and interaction with MRAP.
European journal of pharmacology680(1-3)1-7(2012 Apr)
Reinick CL,Liang L,Angleson JK,Dores RM

Use of chimeric melanocortin-2 and -4 receptors to identify regions responsible for ligand specificity and dependence on melanocortin 2 receptor accessory protein.
European journal of pharmacology660(1)94-102(2011 Jun)
Hinkle PM,Serasinghe MN,Jakabowski A,Sebag JA,Wilson KR,Haskell-Luevano C