Fads1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fads1
Entrez Gene ID	76267
Full Name	fatty acid desaturase 1
Synonyms	0710001O03Rik,A930006B21Rik,AI317215,DSD
General protein information	Preferred Names fatty acid desaturase 1 Names fatty acid desaturase 1 D5D delta(5) desaturase delta(5) fatty acid desaturase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527438.2	XP_006527501.1	acyl-CoA (8-3)-desaturase isoform X1
NM_146094.2	NP_666206.1	acyl-CoA (8-3)-desaturase
XM_006527438.1	XP_006527501.1	fatty acid desaturase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FADS1	NP_037534.3
Canis lupus familiaris (dog)	FADS1	XP_005631706.1
Mus musculus (house mouse)	Fads1	NP_666206.1
Rattus norvegicus (Norway rat)	Fads1	NP_445897.2
Gallus gallus (chicken)	FADS1	XP_421052.4
Bos taurus (cattle)	FADS1	XP_002699331.1
Xenopus tropicalis (tropical clawed frog)	fads1	XP_002943012.2
Pan troglodytes (chimpanzee)	FADS1	XP_001150290.2

Gene Ontology
Bibliography

Related articles in PubMed

Increasing dietary EPA and DHA influence estimated fatty acid desaturase activity in systemic organs which is reflected in the red blood cell in mice.
Davidson EA, Pickens CA, Fenton JI
International journal of food sciences and nutrition69(2)183-191(2018 Mar)

?-5 Fatty Acid Desaturase
Gromovsky AD, Schugar RC, Brown AL, Helsley RN, Burrows AC, Ferguson D, Zhang R, Sansbury BE, Lee RG, Morton RE, Allende DS, Parks JS, Spite M, Brown JM
Arteriosclerosis, thrombosis, and vascular biology38(1)218-231(2018 Jan)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

A Novel Selective Inhibitor of Delta-5 Desaturase Lowers Insulin Resistance and Reduces Body Weight in Diet-Induced Obese C57BL/6J Mice.
Yashiro H, Takagahara S, Tamura YO, Miyahisa I, Matsui J, Suzuki H, Ikeda S, Watanabe M
PloS one11(11)e0166198(2016)

mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle.
Han LQ, Li HJ, Wang YY, Zhu HS, Wang LF, Guo YJ, Lu WF, Wang YL, Yang GY
Genetics and molecular research : GMR9(2)1250-7(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

differential PUFA profiles between HET mice and human FADS SNPs suggest low expression of both FADS1 and 2 genes in human minor haplotypes.
Title: Compensatory induction of Fads1 gene expression in heterozygous Fads2-null mice and by diet with a high n-6/n-3 PUFA ratio.

Data indicate that delta-5 desaturase (D5D) inhibition was confirmed by determining changes in blood arachidonic acid/dihomo-gamma-linolenic acid (AA/DGLA) profiles.
Title: A Novel Selective Inhibitor of Delta-5 Desaturase Lowers Insulin Resistance and Reduces Body Weight in Diet-Induced Obese C57BL/6J Mice.

knockdown of Elovl5, Fads1, or Fads2 decreased the level of Mead acid.
Title: Identification of genes and pathways involved in the synthesis of Mead acid (20:3n-9), an indicator of essential fatty acid deficiency.

The Systemic disruption of the Fads1 gene reciprocally altered the levels of dihomo-gamma-linolenic acid and AA in mouse tissues, resulting in a profound increase in 1-series-derived and a concurrent decrease in 2-series-derived prostaglandins.
Title: Characterization of an arachidonic acid-deficient (Fads1 knockout) mouse model.

analysis of mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle
Title: mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle.

The following Fads1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fads1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14067	NM_146094.2 Latest version!	Mus musculus fatty acid desaturase 1 (Fads1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu55804	XM_006527438.2 Latest version!	Mus musculus fatty acid desaturase 1 (Fads1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu55804	XM_006527438.1	Mus musculus fatty acid desaturase 1 (Fads1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fads1 ( XM_006527438.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527501.1 Mus musculus fatty acid desaturase 1 (Fads1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55804
Clone ID Related Accession (Same CDS sequence)	XM_006527438.2 , XM_006527438.1
Accession Version	XM_006527438.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	fatty acid desaturase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCTCA TGAAGGCCAA CCACCTCTTC TTCCTGGTCT ACCTGCTTCA CATCCTGCTG 
CTGGATGTGG CTGCCTGGCT CACCCTTTGG ATCTTTGGAA CTTCCTTGGT GCCCTTCATC 
CTCTGTGCAG TACTGCTCAG TACAGTTCAG GCTCAGGCAG GTTGGCTACA GCATGACTTT 
GGGCACCTGT CAGTCTTTGG CACCTCGACA TGGAATCACC TGCTACATCA TTTTGTGATT 
GGCCACCTGA AGGGGGCCCC CGCCAGCTGG TGGAACCACA TGCATTTCCA GCACCATGCC 
AAGCCTAACT GCTTCCGCAA GGACCCCGAT ATCAACATGC ACCCCCTCTT CTTCGCCCTG 
GGGAAGGTCC TTCCTGTGGA GCTCGGGAGG GAAAAGAAGA AGCACATGCC ATACAACCAT 
CAGCACAAGT ACTTCTTCCT CATCGGACCC CCAGCCTTGC TGCCTCTATA CTTCCAGTGG 
TATATTTTCT ATTTTGTGGT TCAGCGGAAA AAATGGGTGG ACTTGGCCTG GATGCTCAGC 
TTCTATGCCC GCATCTTCTT CACTTACATG CCGCTGCTGG GGCTGAAAGG CTTCCTGGGC 
CTTTTCTTCA TTGTCAGGTT CCTGGAAAGC AACTGGTTTG TGTGGGTGAC ACAGATGAAC 
CATATCCCCA TGCACATTGA TCACGACCGG AATGTGGACT GGGTCTCCAC CCAGCTGCAG 
GCAACCTGCA ACGTTCACCA ATCAGCCTTC AACAACTGGT TCAGTGGCCA CCTAAATTTC 
CAGATTGAAC ACCACCTCTT CCCCACCATG CCGCGGCACA ACTACCACAA GGTGGCACCC 
CTAGTACAAT CCCTGTGCGC CAAGTACGGC ATCAAGTATG AGTCCAAGCC CCTGCTCACA 
GCCTTCGCGG ACATTGTTTA CTCCCTGAAG GAGTCAGGGC AACTCTGGTT GGACGCTTAC 
CTTCACCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527501.1
CDS	298..1269
Translation	MGLMKANHLFFLVYLLHILLLDVAAWLTLWIFGTSLVPFILCAVLLSTVQAQAGWLQHDFGHLSVFGTSTWNHLLHHFVIGHLKGAPASWWNHMHFQHHAKPNCFRKDPDINMHPLFFALGKVLPVELGREKKKHMPYNHQHKYFFLIGPPALLPLYFQWYIFYFVVQRKKWVDLAWMLSFYARIFFTYMPLLGLKGFLGLFFIVRFLESNWFVWVTQMNHIPMHIDHDRNVDWVSTQLQATCNVHQSAFNNWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKYGIKYESKPLLTAFADIVYSLKESGQLWLDAYLHQ

Target ORF information:

RefSeq Version	XM_006527438.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fatty acid desaturase 1 (Fads1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527438.1

ORF Insert Sequence:

ATGGGCCTCA TGAAGGCCAA CCACCTCTTC TTCCTGGTCT ACCTGCTTCA CATCCTGCTG 
CTGGATGTGG CTGCCTGGCT CACCCTTTGG ATCTTTGGAA CTTCCTTGGT GCCCTTCATC 
CTCTGTGCAG TACTGCTCAG TACAGTTCAG GCTCAGGCAG GTTGGCTACA GCATGACTTT 
GGGCACCTGT CAGTCTTTGG CACCTCGACA TGGAATCACC TGCTACATCA TTTTGTGATT 
GGCCACCTGA AGGGGGCCCC CGCCAGCTGG TGGAACCACA TGCATTTCCA GCACCATGCC 
AAGCCTAACT GCTTCCGCAA GGACCCCGAT ATCAACATGC ACCCCCTCTT CTTCGCCCTG 
GGGAAGGTCC TTCCTGTGGA GCTCGGGAGG GAAAAGAAGA AGCACATGCC ATACAACCAT 
CAGCACAAGT ACTTCTTCCT CATCGGACCC CCAGCCTTGC TGCCTCTATA CTTCCAGTGG 
TATATTTTCT ATTTTGTGGT TCAGCGGAAA AAATGGGTGG ACTTGGCCTG GATGCTCAGC 
TTCTATGCCC GCATCTTCTT CACTTACATG CCGCTGCTGG GGCTGAAAGG CTTCCTGGGC 
CTTTTCTTCA TTGTCAGGTT CCTGGAAAGC AACTGGTTTG TGTGGGTGAC ACAGATGAAC 
CATATCCCCA TGCACATTGA TCACGACCGG AATGTGGACT GGGTCTCCAC CCAGCTGCAG 
GCAACCTGCA ACGTTCACCA ATCAGCCTTC AACAACTGGT TCAGTGGCCA CCTAAATTTC 
CAGATTGAAC ACCACCTCTT CCCCACCATG CCGCGGCACA ACTACCACAA GGTGGCACCC 
CTAGTACAAT CCCTGTGCGC CAAGTACGGC ATCAAGTATG AGTCCAAGCC CCTGCTCACA 
GCCTTCGCGG ACATTGTTTA CTCCCTGAAG GAGTCAGGGC AACTCTGGTT GGACGCTTAC 
CTTCACCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fads1 ( NM_146094.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666206.1 Mus musculus fatty acid desaturase 1 (Fads1), mRNA.

Price & Availability↑

CloneID	OMu14067
Clone ID Related Accession (Same CDS sequence)	NM_146094.2
Accession Version	NM_146094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	acyl-CoA (8-3)-desaturase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132148.3, AK083959.1 and BC063053.1. On Aug 16, 2009 this sequence version replaced NM_146094.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026831.1, AK154367.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCG ACCCGGTGCC GACCCCTGGC CCGGCCTCCG CCCAGCTCCG CCAAACGCGC 
TACTTTACTT GGGAGGAGGT GGCGCAGCGC TCCGGGCGGG AGAAGGAGCG ATGGCTCGTG 
ATCGACCGGA AGGTGTACAA CATCAGCGAC TTCAGCCGCC GCCACCCGGG GGGCTCCCGG 
GTCATCAGCC ACTACGCGGG TCAGGATGCC ACGGATCCTT TTGTGGCATT CCACATCAAC 
AAGGGTCTTG TGAGAAAGTA TATGAACTCT CTTCTGATTG GAGAGCTGGC TCCGGAGCAA 
CCCAGCTTTG AACCCACCAA GAATAAAGCG CTAACTGATG AATTCCGGGA GCTGCGGGCC 
ACAGTAGAGC GAATGGGCCT CATGAAGGCC AACCACCTCT TCTTCCTGGT CTACCTGCTT 
CACATCCTGC TGCTGGATGT GGCTGCCTGG CTCACCCTTT GGATCTTTGG AACTTCCTTG 
GTGCCCTTCA TCCTCTGTGC AGTACTGCTC AGTACAGTTC AGGCTCAGGC AGGTTGGCTA 
CAGCATGACT TTGGGCACCT GTCAGTCTTT GGCACCTCGA CATGGAATCA CCTGCTACAT 
CATTTTGTGA TTGGCCACCT GAAGGGGGCC CCCGCCAGCT GGTGGAACCA CATGCATTTC 
CAGCACCATG CCAAGCCTAA CTGCTTCCGC AAGGACCCCG ATATCAACAT GCACCCCCTC 
TTCTTCGCCC TGGGGAAGGT CCTTCCTGTG GAGCTCGGGA GGGAAAAGAA GAAGCACATG 
CCATACAACC ATCAGCACAA GTACTTCTTC CTCATCGGAC CCCCAGCCTT GCTGCCTCTA 
TACTTCCAGT GGTATATTTT CTATTTTGTG GTTCAGCGGA AAAAATGGGT GGACTTGGCC 
TGGATGCTCA GCTTCTATGC CCGCATCTTC TTCACTTACA TGCCGCTGCT GGGGCTGAAA 
GGCTTCCTGG GCCTTTTCTT CATTGTCAGG TTCCTGGAAA GCAACTGGTT TGTGTGGGTG 
ACACAGATGA ACCATATCCC CATGCACATT GATCACGACC GGAATGTGGA CTGGGTCTCC 
ACCCAGCTGC AGGCAACCTG CAACGTTCAC CAATCAGCCT TCAACAACTG GTTCAGTGGC 
CACCTAAATT TCCAGATTGA ACACCACCTC TTCCCCACCA TGCCGCGGCA CAACTACCAC 
AAGGTGGCAC CCCTAGTACA ATCCCTGTGC GCCAAGTACG GCATCAAGTA TGAGTCCAAG 
CCCCTGCTCA CAGCCTTCGC GGACATTGTT TACTCCCTGA AGGAGTCAGG GCAACTCTGG 
TTGGACGCTT ACCTTCACCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666206.1
CDS	165..1508
Translation	MAPDPVPTPGPASAQLRQTRYFTWEEVAQRSGREKERWLVIDRKVYNISDFSRRHPGGSRVISHYAGQDATDPFVAFHINKGLVRKYMNSLLIGELAPEQPSFEPTKNKALTDEFRELRATVERMGLMKANHLFFLVYLLHILLLDVAAWLTLWIFGTSLVPFILCAVLLSTVQAQAGWLQHDFGHLSVFGTSTWNHLLHHFVIGHLKGAPASWWNHMHFQHHAKPNCFRKDPDINMHPLFFALGKVLPVELGREKKKHMPYNHQHKYFFLIGPPALLPLYFQWYIFYFVVQRKKWVDLAWMLSFYARIFFTYMPLLGLKGFLGLFFIVRFLESNWFVWVTQMNHIPMHIDHDRNVDWVSTQLQATCNVHQSAFNNWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKYGIKYESKPLLTAFADIVYSLKESGQLWLDAYLHQ

Target ORF information:

RefSeq Version	NM_146094.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus fatty acid desaturase 1 (Fads1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146094.2

ORF Insert Sequence:

ATGGCTCCCG ACCCGGTGCC GACCCCTGGC CCGGCCTCCG CCCAGCTCCG CCAAACGCGC 
TACTTTACTT GGGAGGAGGT GGCGCAGCGC TCCGGGCGGG AGAAGGAGCG ATGGCTCGTG 
ATCGACCGGA AGGTGTACAA CATCAGCGAC TTCAGCCGCC GCCACCCGGG GGGCTCCCGG 
GTCATCAGCC ACTACGCGGG TCAGGATGCC ACGGATCCTT TTGTGGCATT CCACATCAAC 
AAGGGTCTTG TGAGAAAGTA TATGAACTCT CTTCTGATTG GAGAGCTGGC TCCGGAGCAA 
CCCAGCTTTG AACCCACCAA GAATAAAGCG CTAACTGATG AATTCCGGGA GCTGCGGGCC 
ACAGTAGAGC GAATGGGCCT CATGAAGGCC AACCACCTCT TCTTCCTGGT CTACCTGCTT 
CACATCCTGC TGCTGGATGT GGCTGCCTGG CTCACCCTTT GGATCTTTGG AACTTCCTTG 
GTGCCCTTCA TCCTCTGTGC AGTACTGCTC AGTACAGTTC AGGCTCAGGC AGGTTGGCTA 
CAGCATGACT TTGGGCACCT GTCAGTCTTT GGCACCTCGA CATGGAATCA CCTGCTACAT 
CATTTTGTGA TTGGCCACCT GAAGGGGGCC CCCGCCAGCT GGTGGAACCA CATGCATTTC 
CAGCACCATG CCAAGCCTAA CTGCTTCCGC AAGGACCCCG ATATCAACAT GCACCCCCTC 
TTCTTCGCCC TGGGGAAGGT CCTTCCTGTG GAGCTCGGGA GGGAAAAGAA GAAGCACATG 
CCATACAACC ATCAGCACAA GTACTTCTTC CTCATCGGAC CCCCAGCCTT GCTGCCTCTA 
TACTTCCAGT GGTATATTTT CTATTTTGTG GTTCAGCGGA AAAAATGGGT GGACTTGGCC 
TGGATGCTCA GCTTCTATGC CCGCATCTTC TTCACTTACA TGCCGCTGCT GGGGCTGAAA 
GGCTTCCTGG GCCTTTTCTT CATTGTCAGG TTCCTGGAAA GCAACTGGTT TGTGTGGGTG 
ACACAGATGA ACCATATCCC CATGCACATT GATCACGACC GGAATGTGGA CTGGGTCTCC 
ACCCAGCTGC AGGCAACCTG CAACGTTCAC CAATCAGCCT TCAACAACTG GTTCAGTGGC 
CACCTAAATT TCCAGATTGA ACACCACCTC TTCCCCACCA TGCCGCGGCA CAACTACCAC 
AAGGTGGCAC CCCTAGTACA ATCCCTGTGC GCCAAGTACG GCATCAAGTA TGAGTCCAAG 
CCCCTGCTCA CAGCCTTCGC GGACATTGTT TACTCCCTGA AGGAGTCAGG GCAACTCTGG 
TTGGACGCTT ACCTTCACCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fads1 ( XM_006527438.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527501.1 Mus musculus fatty acid desaturase 1 (Fads1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55804
Clone ID Related Accession (Same CDS sequence)	XM_006527438.2 , XM_006527438.1
Accession Version	XM_006527438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	acyl-CoA (8-3)-desaturase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCTCA TGAAGGCCAA CCACCTCTTC TTCCTGGTCT ACCTGCTTCA CATCCTGCTG 
CTGGATGTGG CTGCCTGGCT CACCCTTTGG ATCTTTGGAA CTTCCTTGGT GCCCTTCATC 
CTCTGTGCAG TACTGCTCAG TACAGTTCAG GCTCAGGCAG GTTGGCTACA GCATGACTTT 
GGGCACCTGT CAGTCTTTGG CACCTCGACA TGGAATCACC TGCTACATCA TTTTGTGATT 
GGCCACCTGA AGGGGGCCCC CGCCAGCTGG TGGAACCACA TGCATTTCCA GCACCATGCC 
AAGCCTAACT GCTTCCGCAA GGACCCCGAT ATCAACATGC ACCCCCTCTT CTTCGCCCTG 
GGGAAGGTCC TTCCTGTGGA GCTCGGGAGG GAAAAGAAGA AGCACATGCC ATACAACCAT 
CAGCACAAGT ACTTCTTCCT CATCGGACCC CCAGCCTTGC TGCCTCTATA CTTCCAGTGG 
TATATTTTCT ATTTTGTGGT TCAGCGGAAA AAATGGGTGG ACTTGGCCTG GATGCTCAGC 
TTCTATGCCC GCATCTTCTT CACTTACATG CCGCTGCTGG GGCTGAAAGG CTTCCTGGGC 
CTTTTCTTCA TTGTCAGGTT CCTGGAAAGC AACTGGTTTG TGTGGGTGAC ACAGATGAAC 
CATATCCCCA TGCACATTGA TCACGACCGG AATGTGGACT GGGTCTCCAC CCAGCTGCAG 
GCAACCTGCA ACGTTCACCA ATCAGCCTTC AACAACTGGT TCAGTGGCCA CCTAAATTTC 
CAGATTGAAC ACCACCTCTT CCCCACCATG CCGCGGCACA ACTACCACAA GGTGGCACCC 
CTAGTACAAT CCCTGTGCGC CAAGTACGGC ATCAAGTATG AGTCCAAGCC CCTGCTCACA 
GCCTTCGCGG ACATTGTTTA CTCCCTGAAG GAGTCAGGGC AACTCTGGTT GGACGCTTAC 
CTTCACCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527501.1
CDS	622..1593
Translation	MGLMKANHLFFLVYLLHILLLDVAAWLTLWIFGTSLVPFILCAVLLSTVQAQAGWLQHDFGHLSVFGTSTWNHLLHHFVIGHLKGAPASWWNHMHFQHHAKPNCFRKDPDINMHPLFFALGKVLPVELGREKKKHMPYNHQHKYFFLIGPPALLPLYFQWYIFYFVVQRKKWVDLAWMLSFYARIFFTYMPLLGLKGFLGLFFIVRFLESNWFVWVTQMNHIPMHIDHDRNVDWVSTQLQATCNVHQSAFNNWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKYGIKYESKPLLTAFADIVYSLKESGQLWLDAYLHQ

Target ORF information:

RefSeq Version	XM_006527438.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus fatty acid desaturase 1 (Fads1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527438.2

ORF Insert Sequence:

ATGGGCCTCA TGAAGGCCAA CCACCTCTTC TTCCTGGTCT ACCTGCTTCA CATCCTGCTG 
CTGGATGTGG CTGCCTGGCT CACCCTTTGG ATCTTTGGAA CTTCCTTGGT GCCCTTCATC 
CTCTGTGCAG TACTGCTCAG TACAGTTCAG GCTCAGGCAG GTTGGCTACA GCATGACTTT 
GGGCACCTGT CAGTCTTTGG CACCTCGACA TGGAATCACC TGCTACATCA TTTTGTGATT 
GGCCACCTGA AGGGGGCCCC CGCCAGCTGG TGGAACCACA TGCATTTCCA GCACCATGCC 
AAGCCTAACT GCTTCCGCAA GGACCCCGAT ATCAACATGC ACCCCCTCTT CTTCGCCCTG 
GGGAAGGTCC TTCCTGTGGA GCTCGGGAGG GAAAAGAAGA AGCACATGCC ATACAACCAT 
CAGCACAAGT ACTTCTTCCT CATCGGACCC CCAGCCTTGC TGCCTCTATA CTTCCAGTGG 
TATATTTTCT ATTTTGTGGT TCAGCGGAAA AAATGGGTGG ACTTGGCCTG GATGCTCAGC 
TTCTATGCCC GCATCTTCTT CACTTACATG CCGCTGCTGG GGCTGAAAGG CTTCCTGGGC 
CTTTTCTTCA TTGTCAGGTT CCTGGAAAGC AACTGGTTTG TGTGGGTGAC ACAGATGAAC 
CATATCCCCA TGCACATTGA TCACGACCGG AATGTGGACT GGGTCTCCAC CCAGCTGCAG 
GCAACCTGCA ACGTTCACCA ATCAGCCTTC AACAACTGGT TCAGTGGCCA CCTAAATTTC 
CAGATTGAAC ACCACCTCTT CCCCACCATG CCGCGGCACA ACTACCACAA GGTGGCACCC 
CTAGTACAAT CCCTGTGCGC CAAGTACGGC ATCAAGTATG AGTCCAAGCC CCTGCTCACA 
GCCTTCGCGG ACATTGTTTA CTCCCTGAAG GAGTCAGGGC AACTCTGGTT GGACGCTTAC 
CTTCACCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increasing dietary EPA and DHA influence estimated fatty acid desaturase activity in systemic organs which is reflected in the red blood cell in mice.
International journal of food sciences and nutrition69(2)183-191(2018 Mar)
Davidson EA,Pickens CA,Fenton JI

?-5 Fatty Acid Desaturase
Arteriosclerosis, thrombosis, and vascular biology38(1)218-231(2018 Jan)
Gromovsky AD,Schugar RC,Brown AL,Helsley RN,Burrows AC,Ferguson D,Zhang R,Sansbury BE,Lee RG,Morton RE,Allende DS,Parks JS,Spite M,Brown JM

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

A Novel Selective Inhibitor of Delta-5 Desaturase Lowers Insulin Resistance and Reduces Body Weight in Diet-Induced Obese C57BL/6J Mice.
PloS one11(11)e0166198(2016)
Yashiro H,Takagahara S,Tamura YO,Miyahisa I,Matsui J,Suzuki H,Ikeda S,Watanabe M

mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle.
Genetics and molecular research : GMR9(2)1250-7(2010 Jun)
Han LQ,Li HJ,Wang YY,Zhu HS,Wang LF,Guo YJ,Lu WF,Wang YL,Yang GY

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Fads1 cDNA ORF clone, Mus musculus(house mouse)

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ORF Insert Sequence:

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