Rapgef2 cDNA ORF clone, Mus musculus(house mouse)

The following Rapgef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rapgef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu88310 XM_030252869.1
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu32397 XM_006502250.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu32398 XM_006502252.4
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu71469 XM_011240276.3
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu71470 XM_011240277.3
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu21032 NM_001099624.3
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $874.30
$1249.00
OMu32400 NM_001310536.2
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu71468 NM_001310536.1
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu32400 XM_006502254.3
Latest version!
Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OMu71469 XM_011240276.2 Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu32397 XM_006502250.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu71470 XM_011240277.2 Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu32398 XM_006502252.3 Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu88310
    Clone ID Related Accession (Same CDS sequence) XM_030252869.1
    Accession Version XM_030252869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC 
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGAAGAA GAAGTGGCGG AGTCTGGGGT CCCTGAGTCA GGGCAGTGCA
    AACGCCACAG TGCTGGATGT TGCTCAGACA GGCGGGCATA AAAAGCGGGT ACGCCGTAGT
    TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGGAA AGTGAAGCAA
    TACCTGTCCA ACCTGGAGCT GGAGATGGAT GAGGAGAGTC TTCAGACACT GTCACTGCAG
    TGTGAACCAG CAACCAGCAC ATTGCCCAAG AATCCTGGAG ACAAAAAGCC TGTCAAGTCT
    GAGACCTCCC CAGTGGCTCC ACGTGCAGGG CCCCAGCAGA AAGTACAGCC TCAGCAGCCG
    CTGGCACAGC CACAACCTCC ACATAAAGTC AGCCAGGGAC TGCAGGTGCC CGCTGTGTCC
    CTCTATCCTT CACGAAAGAA GGTGCCGGTG AAGGATCTGC CTCCTTTTGG CATAAACTCT
    CCACAAGCTT TGAAGAAAAT CCTGTCCTTG TCTGAAGAAG GGAGTTTGGA GCGTCACAGG
    AAGCAAGCAG AAGATACAAT ATCAAATGCG TCCTCTCAGC TCTCATCGCC CCCCACCTCA
    CCTCAGAGCT CTCCAAGGAA AGGTTATGCA CTGGCTCTCA GTGGCACCGT GGATAATTTC
    TCAGATTCAG GTCACAGTGA GATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCTTTT
    GACTCGGTGC CAGTCTCTCT GCATGATGAA CGGCGCCAGA GGCATTCTGT CAGCATTGTG
    GAGAGCAACC TAGGTGTGGG CAGGATGGAG CGGCGGACCC TGATGGAGCC TGATCAATAC
    AGCTTAGGGT CGTATGCCCC AGTGTCTGAA AGCCGAGGCC TGTATGCCGC AGCGACGGTC
    ATCTCTTCTC CTAGCACAGA GGAGCTTTCC CATGACCAAG GGGACCGTGC TTCCCTCGAT
    GCTGCTGACA GCGGTCGTGG GAGCTGGACA TCATGCTCAA GTGGTTCCCA TGACAACATA
    CAGACCATTC AGCACCAGAG AAGCTGGGAA ACACTGCCAT TTGGGCACAC TCACTTTGAC
    TATTCAGGGG ATGCTGCAAG TATATGGGCC TCCGGTGGCC ATATGGACCA AATGATGTTT
    TCTGATCATA GCACAAAATA TAACAGACAG AATCAAAGTA GAGAGAGTCT TGAACAAGCC
    CAATCCCGGG CCAGCTGGGC GTCTTCCACG GGCTACTGGG GAGAAGACTC AGAAGGCGAC
    ACAGGCACTA TCAAGCGAAG GGGTGGGAAG GATGTCTCCG CTGAGGCAGA GAGCAGCAGC
    ATGGTGCCCG TGACTACAGA GGAAGCCAAA CCTGTCCCTA TGCCTGCCCA CATAGCTGTG
    ACGCCGAGCA CTACCAAGGG ACTCATCGCA CGGAAGGAAG GCAGGTACCG GGAGCCGCCT
    CCCACACCTC CAGGCTACGT GGGCATCCCC ATTGCCGATT TCCCAGAAGG GCCTTGCCAC
    CCGGCCAGGA AGCCCCCGGA TTACAACGTG GCCCTGCAGC GGTCCCGCAT GGTGGCACGG
    CCCACTGAGG CCCCGGCACC GGGCCAGACG CCGCCTGCAG CCGCAGCCAG CCGGCCGGGC
    AGCAAGCCAC AGTGGCACAA GCCCAGCGAC GCAGACCCAC GCCTCGCGCC CTTCCAGCCG
    CAGGGCTTCG CAGGAGCGGA GGAGGACGAA GATGAACAAG TGTCTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030108729.1
    CDS14110..18543
    Translation

    Target ORF information:

    RefSeq Version XM_030252869.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030252869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC 
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGAAGAA GAAGTGGCGG AGTCTGGGGT CCCTGAGTCA GGGCAGTGCA
    AACGCCACAG TGCTGGATGT TGCTCAGACA GGCGGGCATA AAAAGCGGGT ACGCCGTAGT
    TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGGAA AGTGAAGCAA
    TACCTGTCCA ACCTGGAGCT GGAGATGGAT GAGGAGAGTC TTCAGACACT GTCACTGCAG
    TGTGAACCAG CAACCAGCAC ATTGCCCAAG AATCCTGGAG ACAAAAAGCC TGTCAAGTCT
    GAGACCTCCC CAGTGGCTCC ACGTGCAGGG CCCCAGCAGA AAGTACAGCC TCAGCAGCCG
    CTGGCACAGC CACAACCTCC ACATAAAGTC AGCCAGGGAC TGCAGGTGCC CGCTGTGTCC
    CTCTATCCTT CACGAAAGAA GGTGCCGGTG AAGGATCTGC CTCCTTTTGG CATAAACTCT
    CCACAAGCTT TGAAGAAAAT CCTGTCCTTG TCTGAAGAAG GGAGTTTGGA GCGTCACAGG
    AAGCAAGCAG AAGATACAAT ATCAAATGCG TCCTCTCAGC TCTCATCGCC CCCCACCTCA
    CCTCAGAGCT CTCCAAGGAA AGGTTATGCA CTGGCTCTCA GTGGCACCGT GGATAATTTC
    TCAGATTCAG GTCACAGTGA GATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCTTTT
    GACTCGGTGC CAGTCTCTCT GCATGATGAA CGGCGCCAGA GGCATTCTGT CAGCATTGTG
    GAGAGCAACC TAGGTGTGGG CAGGATGGAG CGGCGGACCC TGATGGAGCC TGATCAATAC
    AGCTTAGGGT CGTATGCCCC AGTGTCTGAA AGCCGAGGCC TGTATGCCGC AGCGACGGTC
    ATCTCTTCTC CTAGCACAGA GGAGCTTTCC CATGACCAAG GGGACCGTGC TTCCCTCGAT
    GCTGCTGACA GCGGTCGTGG GAGCTGGACA TCATGCTCAA GTGGTTCCCA TGACAACATA
    CAGACCATTC AGCACCAGAG AAGCTGGGAA ACACTGCCAT TTGGGCACAC TCACTTTGAC
    TATTCAGGGG ATGCTGCAAG TATATGGGCC TCCGGTGGCC ATATGGACCA AATGATGTTT
    TCTGATCATA GCACAAAATA TAACAGACAG AATCAAAGTA GAGAGAGTCT TGAACAAGCC
    CAATCCCGGG CCAGCTGGGC GTCTTCCACG GGCTACTGGG GAGAAGACTC AGAAGGCGAC
    ACAGGCACTA TCAAGCGAAG GGGTGGGAAG GATGTCTCCG CTGAGGCAGA GAGCAGCAGC
    ATGGTGCCCG TGACTACAGA GGAAGCCAAA CCTGTCCCTA TGCCTGCCCA CATAGCTGTG
    ACGCCGAGCA CTACCAAGGG ACTCATCGCA CGGAAGGAAG GCAGGTACCG GGAGCCGCCT
    CCCACACCTC CAGGCTACGT GGGCATCCCC ATTGCCGATT TCCCAGAAGG GCCTTGCCAC
    CCGGCCAGGA AGCCCCCGGA TTACAACGTG GCCCTGCAGC GGTCCCGCAT GGTGGCACGG
    CCCACTGAGG CCCCGGCACC GGGCCAGACG CCGCCTGCAG CCGCAGCCAG CCGGCCGGGC
    AGCAAGCCAC AGTGGCACAA GCCCAGCGAC GCAGACCCAC GCCTCGCGCC CTTCCAGCCG
    CAGGGCTTCG CAGGAGCGGA GGAGGACGAA GATGAACAAG TGTCTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32397
    Clone ID Related Accession (Same CDS sequence) XM_006502250.3 , XM_006502250.4
    Accession Version XM_006502250.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502250.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGCTCCC TGCAGACTTC
    ACAAAACTGC ACCTCACTGA CAGCCTGCAC CCACAGGTGA CCCACGTGTC CTCCAGCCAT
    TCAGGATGTA GCATCACCAG CGACTCTGGG AGCAGCAGTC TTTCTGACAT TTACCAGGCC
    ACGGAGAGCG AGGCCGGTGA CATGGACCTG AGTGGACTGC CAGAGACGGC GGTTGACTCT
    GAGGATGACG ATGATGAGGA AGACATCGAG AGAGCATCAG ACCCTCTGAT GAGCAGGGAC
    ATCGTGAGGG ACTGCCTGGA GAAGGACCCG ATAGACAGGA CTGACGATGA CATCGAACAA
    CTACTGGAAT TTATGCACCA GCTGCCGGCC TTTGCCAATA TGACGATGTC AGTAAGACGG
    GAGCTCTGTG CCGTGATGGT GTTTGCAGTG GTGGAAAGAG CTGGAACCAT TGTGTTAAAC
    GACGGAGAAG AGCTGGATTC CTGGTCAGTG ATTCTCAATG GTTCCGTGGA GGTGACATAT
    CCGGATGGGA AAGCAGAAAT TCTGTGCATG GGAAACAGCT TTGGTGTTTC GCCCACCATG
    GACAAAGAAT ACATGAAAGG AGTCATGAGG ACTAAGGTGG ATGACTGCCA GTTTGTCTGC
    ATTGCCCAGC AAGATTACTG CCGTATTTTG AACCAAGTAG AAAAGAACAT GCAAAAAGTT
    GAGGAGGAAG GAGAGATTGT AATGGTGAAG GAGCACCGAG AACTTGATCG GACTGGAACA
    AGGAAGGGCC ACATCGTCAT CAAGGGCACC TCAGAGAGGC TGACCATGCA TTTGGTGGAA
    GAGCATTCTG TAGTGGATCC AACATTCATA GAAGACTTCC TGTTGACATA CCGGACTTTC
    CTTTCCAGCC CGATGGAAGT GGGCAAGAAG TTATTGGAGT GGTTCAATGA CCCGAGCCTC
    AGGGATAAGG TGACGCGGGT AGTATTACTG TGGGTGAATA ATCACTTCAA TGACTTTGAA
    GGGGATCCTG CAATGACTCG ATTTCTGGAA GAATTTGAAA ATAATCTGGA AAGAGAGAAA
    ATGGGTGGAC ATCTAAGGCT GTTGAACATT GCATGTGCAG CTAAAGCCAA ACGAAGACTG
    ATGACGTTAA CAAAGCCTTC CCGGGAAGCC CCTCTGCCCT TCATCTTACT GGGAGGCTCT
    GAAAAGGGCT TTGGCATCTT CGTGGACAGC GTTGACTCGT GTAGCAAAGC CACTGAAGCA
    GGCTTGAAGC GCGGGGATCA GATATTAGAA GTGAATGGTC AAAATTTTGA AAATATCCAA
    CTTTCAAAGG CAATGGAAAT TCTCAGAAAT AACACACACC TGTCCATCAC TGTGAAAACC
    AACTTATTTG TATTTAAAGA ACTTCTGACG AGATTGTCTG AAGAGAAAAG AAACGGTGCC
    CCTCACCTCC CTAAAATCGG GGACATCAAG AAGGCCAGCC GCTACTCCAT ACCGGATCTC
    GCCGTAGACG TAGAACAGGT GATAGGCCTC GAGAAGGTCA ACAAGAAAAG CAAAGCCAAC
    ACCGTGGGAG GAAGAAACAA GCTGAAGAAA ATACTCGACA AGACCCGGAT CAGCATCTTA
    CCTCAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGATAG CATCGTAGGA
    CTGAGGCAGA CCAAGCACAT CCCAGCTGCC CTGCCTGTCA GTGGAACGCT GTCCTCCAGT
    AACCCTGACT TGCTGCAGTC ACACCACCGG ATTCTGGACT TCAGCACTAC TCCTGACTTG
    CCAGATCAGG TACTGAGAGT TTTCAAGGCT GACCAACAAA GCAGGTACAT CATGATCAGC
    AAGGACACCA CCGCAAAGGA AGTGGTCATC CAGGCCATCA GAGAGTTTGC TGTCACTGCC
    ACCCCAGAGC AGTATTCCCT CTGTGAGGTC TCAGTCACAC CAGAGGGAGT TATCAAACAA
    CGCCGACTCC CAGATCAGCT TTCCAAGCTT GCCGACAGGA TACAACTGAG TGGAAGGTAT
    TATCTGAAAA ACAACATGGA AACAGAAACG CTTTGCTCAG ATGAAGATGC CCAGGAGCTG
    CTGCGAGAGA GCCAAATCTC CCTCCTTCAG CTCAGCACCG TGGAGGTTGC CACGCAGCTC
    TCCATGAGGA ACTTTGAACT CTTCCGCAAC ATTGAGCCTA CTGAATACAT AGATGATTTA
    TTTAAACTCA AATCAAAAAC CAGCTGTGCC AACCTGAAGA AATTTGAGGA AGTCATTAAC
    CAGGAAACAT TTTGGGTGGC TTCTGAAATC CTGCGGGAAA CAAACCAGCT GAAGAGGATG
    AAGATCATTA AGCATTTCAT CAAGATTGCC TTGCACTGCA GGGAATGCAA GAATTTTAAC
    TCCATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCGG TGGCAAGACT ACGAACCACC
    TGGGAAAAGC TTCCCAATAA ATATGAAAAG CTGTTCCAGG ACCTGCAAGA CCTGTTTGAT
    CCTTCCAGAA ACATGGCAAA GTACCGCAAT GTCCTGAGTG GCCAGAACTT ACAGCCTCCT
    GTCATTCCCC TGTTCCCTGT CATCAAGAAG GACCTCACGT TTCTCCATGA GGGAAATGAC
    TCAAAAGTAG ACGGACTGGT CAACTTTGAG AAGCTGAGGA TGATTGCAAA AGAAATCCGT
    CACGTTGGCC GAATGGCATC AGTCAACATG GACCCTGCCC TTATGTTCAG GACTCGGAAG
    AAGAAGTGGC GGAGTCTGGG GTCCCTGAGT CAGGGCAGTG CAAACGCCAC AGTGCTGGAT
    GTTGCTCAGA CAGGCGGGCA TAAAAAGCGG GTACGCCGTA GTTCCTTCCT CAATGCCAAA
    AAGCTGTATG AAGATGCCCA GATGGCTCGG AAAGTGAAGC AATACCTGTC CAACCTGGAG
    CTGGAGATGG ATGAGGAGAG TCTTCAGACA CTGTCACTGC AGTGTGAACC AGCAACCAGC
    ACATTGCCCA AGAATCCTGG AGACAAAAAG CCTGTCAAGT CTGAGACCTC CCCAGTGGCT
    CCACGTGCAG GGCCCCAGCA GAAAGTACAG CCTCAGCAGC CGCTGGCACA GCCACAACCT
    CCACATAAAG TCAGCCAGGG ACTGCAGGTG CCCGCTGTGT CCCTCTATCC TTCACGAAAG
    AAGGTGCCGG TGAAGGATCT GCCTCCTTTT GGCATAAACT CTCCACAAGC TTTGAAGAAA
    ATCCTGTCCT TGTCTGAAGA AGGGAGTTTG GAGCGTCACA GGAAGCAAGC AGAAGATACA
    ATATCAAATG CGTCCTCTCA GCTCTCATCG CCCCCCACCT CACCTCAGAG CTCTCCAAGG
    AAAGGTTATG CACTGGCTCT CAGTGGCACC GTGGATAATT TCTCAGATTC AGGTCACAGT
    GAGATTTCTT CACGATCCAG TATTGTCAGC AATTCTTCTT TTGACTCGGT GCCAGTCTCT
    CTGCATGATG AACGGCGCCA GAGGCATTCT GTCAGCATTG TGGAGAGCAA CCTAGGTGTG
    GGCAGGATGG AGCGGCGGAC CCTGATGGAG CCTGATCAAT ACAGCTTAGG GTCGTATGCC
    CCAGTGTCTG AAAGCCGAGG CCTGTATGCC GCAGCGACGG TCATCTCTTC TCCTAGCACA
    GAGGAGCTTT CCCATGACCA AGGGGACCGT GCTTCCCTCG ATGCTGCTGA CAGCGGTCGT
    GGGAGCTGGA CATCATGCTC AAGTGGTTCC CATGACAACA TACAGACCAT TCAGCACCAG
    AGAAGCTGGG AAACACTGCC ATTTGGGCAC ACTCACTTTG ACTATTCAGG GGATGCTGCA
    AGTATATGGG CCTCCGGTGG CCATATGGAC CAAATGATGT TTTCTGATCA TAGCACAAAA
    TATAACAGAC AGAATCAAAG TAGAGAGAGT CTTGAACAAG CCCAATCCCG GGCCAGCTGG
    GCGTCTTCCA CGGGCTACTG GGGAGAAGAC TCAGAAGGCG ACACAGGCAC TATCAAGCGA
    AGGGGTGGGA AGGATGTCTC CGCTGAGGCA GAGAGCAGCA GCATGGTGCC CGTGACTACA
    GAGGAAGCCA AACCTGTCCC TATGCCTGCC CACATAGCTG TGACGCCGAG CACTACCAAG
    GGACTCATCG CACGGAAGGA AGGCAGGTAC CGGGAGCCGC CTCCCACACC TCCAGGCTAC
    GTGGGCATCC CCATTGCCGA TTTCCCAGAA GGGCCTTGCC ACCCGGCCAG GAAGCCCCCG
    GATTACAACG TGGCCCTGCA GCGGTCCCGC ATGGTGGCAC GGCCCACTGA GGCCCCGGCA
    CCGGGCCAGA CGCCGCCTGC AGCCGCAGCC AGCCGGCCGG GCAGCAAGCC ACAGTGGCAC
    AAGCCCAGCG ACGCAGACCC ACGCCTCGCG CCCTTCCAGC CGCAGGGCTT CGCAGGAGCG
    GAGGAGGACG AAGATGAACA AGTGTCTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006502313.1
    CDS14..4969
    Translation

    Target ORF information:

    RefSeq Version XM_006502250.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006502250.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGCTCCC TGCAGACTTC
    ACAAAACTGC ACCTCACTGA CAGCCTGCAC CCACAGGTGA CCCACGTGTC CTCCAGCCAT
    TCAGGATGTA GCATCACCAG CGACTCTGGG AGCAGCAGTC TTTCTGACAT TTACCAGGCC
    ACGGAGAGCG AGGCCGGTGA CATGGACCTG AGTGGACTGC CAGAGACGGC GGTTGACTCT
    GAGGATGACG ATGATGAGGA AGACATCGAG AGAGCATCAG ACCCTCTGAT GAGCAGGGAC
    ATCGTGAGGG ACTGCCTGGA GAAGGACCCG ATAGACAGGA CTGACGATGA CATCGAACAA
    CTACTGGAAT TTATGCACCA GCTGCCGGCC TTTGCCAATA TGACGATGTC AGTAAGACGG
    GAGCTCTGTG CCGTGATGGT GTTTGCAGTG GTGGAAAGAG CTGGAACCAT TGTGTTAAAC
    GACGGAGAAG AGCTGGATTC CTGGTCAGTG ATTCTCAATG GTTCCGTGGA GGTGACATAT
    CCGGATGGGA AAGCAGAAAT TCTGTGCATG GGAAACAGCT TTGGTGTTTC GCCCACCATG
    GACAAAGAAT ACATGAAAGG AGTCATGAGG ACTAAGGTGG ATGACTGCCA GTTTGTCTGC
    ATTGCCCAGC AAGATTACTG CCGTATTTTG AACCAAGTAG AAAAGAACAT GCAAAAAGTT
    GAGGAGGAAG GAGAGATTGT AATGGTGAAG GAGCACCGAG AACTTGATCG GACTGGAACA
    AGGAAGGGCC ACATCGTCAT CAAGGGCACC TCAGAGAGGC TGACCATGCA TTTGGTGGAA
    GAGCATTCTG TAGTGGATCC AACATTCATA GAAGACTTCC TGTTGACATA CCGGACTTTC
    CTTTCCAGCC CGATGGAAGT GGGCAAGAAG TTATTGGAGT GGTTCAATGA CCCGAGCCTC
    AGGGATAAGG TGACGCGGGT AGTATTACTG TGGGTGAATA ATCACTTCAA TGACTTTGAA
    GGGGATCCTG CAATGACTCG ATTTCTGGAA GAATTTGAAA ATAATCTGGA AAGAGAGAAA
    ATGGGTGGAC ATCTAAGGCT GTTGAACATT GCATGTGCAG CTAAAGCCAA ACGAAGACTG
    ATGACGTTAA CAAAGCCTTC CCGGGAAGCC CCTCTGCCCT TCATCTTACT GGGAGGCTCT
    GAAAAGGGCT TTGGCATCTT CGTGGACAGC GTTGACTCGT GTAGCAAAGC CACTGAAGCA
    GGCTTGAAGC GCGGGGATCA GATATTAGAA GTGAATGGTC AAAATTTTGA AAATATCCAA
    CTTTCAAAGG CAATGGAAAT TCTCAGAAAT AACACACACC TGTCCATCAC TGTGAAAACC
    AACTTATTTG TATTTAAAGA ACTTCTGACG AGATTGTCTG AAGAGAAAAG AAACGGTGCC
    CCTCACCTCC CTAAAATCGG GGACATCAAG AAGGCCAGCC GCTACTCCAT ACCGGATCTC
    GCCGTAGACG TAGAACAGGT GATAGGCCTC GAGAAGGTCA ACAAGAAAAG CAAAGCCAAC
    ACCGTGGGAG GAAGAAACAA GCTGAAGAAA ATACTCGACA AGACCCGGAT CAGCATCTTA
    CCTCAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGATAG CATCGTAGGA
    CTGAGGCAGA CCAAGCACAT CCCAGCTGCC CTGCCTGTCA GTGGAACGCT GTCCTCCAGT
    AACCCTGACT TGCTGCAGTC ACACCACCGG ATTCTGGACT TCAGCACTAC TCCTGACTTG
    CCAGATCAGG TACTGAGAGT TTTCAAGGCT GACCAACAAA GCAGGTACAT CATGATCAGC
    AAGGACACCA CCGCAAAGGA AGTGGTCATC CAGGCCATCA GAGAGTTTGC TGTCACTGCC
    ACCCCAGAGC AGTATTCCCT CTGTGAGGTC TCAGTCACAC CAGAGGGAGT TATCAAACAA
    CGCCGACTCC CAGATCAGCT TTCCAAGCTT GCCGACAGGA TACAACTGAG TGGAAGGTAT
    TATCTGAAAA ACAACATGGA AACAGAAACG CTTTGCTCAG ATGAAGATGC CCAGGAGCTG
    CTGCGAGAGA GCCAAATCTC CCTCCTTCAG CTCAGCACCG TGGAGGTTGC CACGCAGCTC
    TCCATGAGGA ACTTTGAACT CTTCCGCAAC ATTGAGCCTA CTGAATACAT AGATGATTTA
    TTTAAACTCA AATCAAAAAC CAGCTGTGCC AACCTGAAGA AATTTGAGGA AGTCATTAAC
    CAGGAAACAT TTTGGGTGGC TTCTGAAATC CTGCGGGAAA CAAACCAGCT GAAGAGGATG
    AAGATCATTA AGCATTTCAT CAAGATTGCC TTGCACTGCA GGGAATGCAA GAATTTTAAC
    TCCATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCGG TGGCAAGACT ACGAACCACC
    TGGGAAAAGC TTCCCAATAA ATATGAAAAG CTGTTCCAGG ACCTGCAAGA CCTGTTTGAT
    CCTTCCAGAA ACATGGCAAA GTACCGCAAT GTCCTGAGTG GCCAGAACTT ACAGCCTCCT
    GTCATTCCCC TGTTCCCTGT CATCAAGAAG GACCTCACGT TTCTCCATGA GGGAAATGAC
    TCAAAAGTAG ACGGACTGGT CAACTTTGAG AAGCTGAGGA TGATTGCAAA AGAAATCCGT
    CACGTTGGCC GAATGGCATC AGTCAACATG GACCCTGCCC TTATGTTCAG GACTCGGAAG
    AAGAAGTGGC GGAGTCTGGG GTCCCTGAGT CAGGGCAGTG CAAACGCCAC AGTGCTGGAT
    GTTGCTCAGA CAGGCGGGCA TAAAAAGCGG GTACGCCGTA GTTCCTTCCT CAATGCCAAA
    AAGCTGTATG AAGATGCCCA GATGGCTCGG AAAGTGAAGC AATACCTGTC CAACCTGGAG
    CTGGAGATGG ATGAGGAGAG TCTTCAGACA CTGTCACTGC AGTGTGAACC AGCAACCAGC
    ACATTGCCCA AGAATCCTGG AGACAAAAAG CCTGTCAAGT CTGAGACCTC CCCAGTGGCT
    CCACGTGCAG GGCCCCAGCA GAAAGTACAG CCTCAGCAGC CGCTGGCACA GCCACAACCT
    CCACATAAAG TCAGCCAGGG ACTGCAGGTG CCCGCTGTGT CCCTCTATCC TTCACGAAAG
    AAGGTGCCGG TGAAGGATCT GCCTCCTTTT GGCATAAACT CTCCACAAGC TTTGAAGAAA
    ATCCTGTCCT TGTCTGAAGA AGGGAGTTTG GAGCGTCACA GGAAGCAAGC AGAAGATACA
    ATATCAAATG CGTCCTCTCA GCTCTCATCG CCCCCCACCT CACCTCAGAG CTCTCCAAGG
    AAAGGTTATG CACTGGCTCT CAGTGGCACC GTGGATAATT TCTCAGATTC AGGTCACAGT
    GAGATTTCTT CACGATCCAG TATTGTCAGC AATTCTTCTT TTGACTCGGT GCCAGTCTCT
    CTGCATGATG AACGGCGCCA GAGGCATTCT GTCAGCATTG TGGAGAGCAA CCTAGGTGTG
    GGCAGGATGG AGCGGCGGAC CCTGATGGAG CCTGATCAAT ACAGCTTAGG GTCGTATGCC
    CCAGTGTCTG AAAGCCGAGG CCTGTATGCC GCAGCGACGG TCATCTCTTC TCCTAGCACA
    GAGGAGCTTT CCCATGACCA AGGGGACCGT GCTTCCCTCG ATGCTGCTGA CAGCGGTCGT
    GGGAGCTGGA CATCATGCTC AAGTGGTTCC CATGACAACA TACAGACCAT TCAGCACCAG
    AGAAGCTGGG AAACACTGCC ATTTGGGCAC ACTCACTTTG ACTATTCAGG GGATGCTGCA
    AGTATATGGG CCTCCGGTGG CCATATGGAC CAAATGATGT TTTCTGATCA TAGCACAAAA
    TATAACAGAC AGAATCAAAG TAGAGAGAGT CTTGAACAAG CCCAATCCCG GGCCAGCTGG
    GCGTCTTCCA CGGGCTACTG GGGAGAAGAC TCAGAAGGCG ACACAGGCAC TATCAAGCGA
    AGGGGTGGGA AGGATGTCTC CGCTGAGGCA GAGAGCAGCA GCATGGTGCC CGTGACTACA
    GAGGAAGCCA AACCTGTCCC TATGCCTGCC CACATAGCTG TGACGCCGAG CACTACCAAG
    GGACTCATCG CACGGAAGGA AGGCAGGTAC CGGGAGCCGC CTCCCACACC TCCAGGCTAC
    GTGGGCATCC CCATTGCCGA TTTCCCAGAA GGGCCTTGCC ACCCGGCCAG GAAGCCCCCG
    GATTACAACG TGGCCCTGCA GCGGTCCCGC ATGGTGGCAC GGCCCACTGA GGCCCCGGCA
    CCGGGCCAGA CGCCGCCTGC AGCCGCAGCC AGCCGGCCGG GCAGCAAGCC ACAGTGGCAC
    AAGCCCAGCG ACGCAGACCC ACGCCTCGCG CCCTTCCAGC CGCAGGGCTT CGCAGGAGCG
    GAGGAGGACG AAGATGAACA AGTGTCTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32398
    Clone ID Related Accession (Same CDS sequence) XM_006502252.3 , XM_006502252.4
    Accession Version XM_006502252.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502252.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TCCAACGTCA AGCCTCCCAC 
    AGACAGTCAC GGAGAAGATT TAGAAAGATC AACCAGAAAG GTGAAAGACA AACAATTATC
    GACACAGTGG ACCCTTACCC TGTGGGCAAG CCACCTTTAC CTAGAGGCTA CCATACGGAA
    TGCACTAAAT CTCAGCTCCC TGCAGACTTC ACAAAACTGC ACCTCACTGA CAGCCTGCAC
    CCACAGGTGA CCCACGTGTC CTCCAGCCAT TCAGGATGTA GCATCACCAG CGACTCTGGG
    AGCAGCAGTC TTTCTGACAT TTACCAGGCC ACGGAGAGCG AGGCCGGTGA CATGGACCTG
    AGTGGACTGC CAGAGACGGC GGTTGACTCT GAGGATGACG ATGATGAGGA AGACATCGAG
    AGAGCATCAG ACCCTCTGAT GAGCAGGGAC ATCGTGAGGG ACTGCCTGGA GAAGGACCCG
    ATAGACAGGA CTGACGATGA CATCGAACAA CTACTGGAAT TTATGCACCA GCTGCCGGCC
    TTTGCCAATA TGACGATGTC AGTAAGACGG GAGCTCTGTG CCGTGATGGT GTTTGCAGTG
    GTGGAAAGAG CTGGAACCAT TGTGTTAAAC GACGGAGAAG AGCTGGATTC CTGGTCAGTG
    ATTCTCAATG GTTCCGTGGA GGTGACATAT CCGGATGGGA AAGCAGAAAT TCTGTGCATG
    GGAAACAGCT TTGGTGTTTC GCCCACCATG GACAAAGAAT ACATGAAAGG AGTCATGAGG
    ACTAAGGTGG ATGACTGCCA GTTTGTCTGC ATTGCCCAGC AAGATTACTG CCGTATTTTG
    AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAGGAGGAAG GAGAGATTGT AATGGTGAAG
    GAGCACCGAG AACTTGATCG GACTGGAACA AGGAAGGGCC ACATCGTCAT CAAGGGCACC
    TCAGAGAGGC TGACCATGCA TTTGGTGGAA GAGCATTCTG TAGTGGATCC AACATTCATA
    GAAGACTTCC TGTTGACATA CCGGACTTTC CTTTCCAGCC CGATGGAAGT GGGCAAGAAG
    TTATTGGAGT GGTTCAATGA CCCGAGCCTC AGGGATAAGG TGACGCGGGT AGTATTACTG
    TGGGTGAATA ATCACTTCAA TGACTTTGAA GGGGATCCTG CAATGACTCG ATTTCTGGAA
    GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTGAACATT
    GCATGTGCAG CTAAAGCCAA ACGAAGACTG ATGACGTTAA CAAAGCCTTC CCGGGAAGCC
    CCTCTGCCCT TCATCTTACT GGGAGGCTCT GAAAAGGGCT TTGGCATCTT CGTGGACAGC
    GTTGACTCGT GTAGCAAAGC CACTGAAGCA GGCTTGAAGC GCGGGGATCA GATATTAGAA
    GTGAATGGTC AAAATTTTGA AAATATCCAA CTTTCAAAGG CAATGGAAAT TCTCAGAAAT
    AACACACACC TGTCCATCAC TGTGAAAACC AACTTATTTG TATTTAAAGA ACTTCTGACG
    AGATTGTCTG AAGAGAAAAG AAACGGTGCC CCTCACCTCC CTAAAATCGG GGACATCAAG
    AAGGCCAGCC GCTACTCCAT ACCGGATCTC GCCGTAGACG TAGAACAGGT GATAGGCCTC
    GAGAAGGTCA ACAAGAAAAG CAAAGCCAAC ACCGTGGGAG GAAGAAACAA GCTGAAGAAA
    ATACTCGACA AGACCCGGAT CAGCATCTTA CCTCAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGATAG CATCGTAGGA CTGAGGCAGA CCAAGCACAT CCCAGCTGCC
    CTGCCTGTCA GTGGAACGCT GTCCTCCAGT AACCCTGACT TGCTGCAGTC ACACCACCGG
    ATTCTGGACT TCAGCACTAC TCCTGACTTG CCAGATCAGG TACTGAGAGT TTTCAAGGCT
    GACCAACAAA GCAGGTACAT CATGATCAGC AAGGACACCA CCGCAAAGGA AGTGGTCATC
    CAGGCCATCA GAGAGTTTGC TGTCACTGCC ACCCCAGAGC AGTATTCCCT CTGTGAGGTC
    TCAGTCACAC CAGAGGGAGT TATCAAACAA CGCCGACTCC CAGATCAGCT TTCCAAGCTT
    GCCGACAGGA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACG
    CTTTGCTCAG ATGAAGATGC CCAGGAGCTG CTGCGAGAGA GCCAAATCTC CCTCCTTCAG
    CTCAGCACCG TGGAGGTTGC CACGCAGCTC TCCATGAGGA ACTTTGAACT CTTCCGCAAC
    ATTGAGCCTA CTGAATACAT AGATGATTTA TTTAAACTCA AATCAAAAAC CAGCTGTGCC
    AACCTGAAGA AATTTGAGGA AGTCATTAAC CAGGAAACAT TTTGGGTGGC TTCTGAAATC
    CTGCGGGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATTGCC
    TTGCACTGCA GGGAATGCAA GAATTTTAAC TCCATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCGG TGGCAAGACT ACGAACCACC TGGGAAAAGC TTCCCAATAA ATATGAAAAG
    CTGTTCCAGG ACCTGCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA GTACCGCAAT
    GTCCTGAGTG GCCAGAACTT ACAGCCTCCT GTCATTCCCC TGTTCCCTGT CATCAAGAAG
    GACCTCACGT TTCTCCATGA GGGAAATGAC TCAAAAGTAG ACGGACTGGT CAACTTTGAG
    AAGCTGAGGA TGATTGCAAA AGAAATCCGT CACGTTGGCC GAATGGCATC AGTCAACATG
    GACCCTGCCC TTATGTTCAG GACTCGGAAG AAGAAGTGGC GGAGTCTGGG GTCCCTGAGT
    CAGGGCAGTG CAAACGCCAC AGTGCTGGAT GTTGCTCAGA CAGGCGGGCA TAAAAAGCGG
    GTACGCCGTA GTTCCTTCCT CAATGCCAAA AAGCTGTATG AAGATGCCCA GATGGCTCGG
    AAAGTGAAGC AATACCTGTC CAACCTGGAG CTGGAGATGG ATGAGGAGAG TCTTCAGACA
    CTGTCACTGC AGTGTGAACC AGCAACCAGC ACATTGCCCA AGAATCCTGG AGACAAAAAG
    CCTGTCAAGT CTGAGACCTC CCCAGTGGCT CCACGTGCAG GGCCCCAGCA GAAAGTACAG
    CCTCAGCAGC CGCTGGCACA GCCACAACCT CCACATAAAG TCAGCCAGGG ACTGCAGGTG
    CCCGCTGTGT CCCTCTATCC TTCACGAAAG AAGGTGCCGG TGAAGGATCT GCCTCCTTTT
    GGCATAAACT CTCCACAAGC TTTGAAGAAA ATCCTGTCCT TGTCTGAAGA AGGGAGTTTG
    GAGCGTCACA GGAAGCAAGC AGAAGATACA ATATCAAATG CGTCCTCTCA GCTCTCATCG
    CCCCCCACCT CACCTCAGAG CTCTCCAAGG AAAGGTTATG CACTGGCTCT CAGTGGCACC
    GTGGATAATT TCTCAGATTC AGGTCACAGT GAGATTTCTT CACGATCCAG TATTGTCAGC
    AATTCTTCTT TTGACTCGGT GCCAGTCTCT CTGCATGATG AACGGCGCCA GAGGCATTCT
    GTCAGCATTG TGGAGAGCAA CCTAGGTGTG GGCAGGATGG AGCGGCGGAC CCTGATGGAG
    CCTGATCAAT ACAGCTTAGG GTCGTATGCC CCAGTGTCTG AAAGCCGAGG CCTGTATGCC
    GCAGCGACGG TCATCTCTTC TCCTAGCACA GAGGAGCTTT CCCATGACCA AGGGGACCGT
    GCTTCCCTCG ATGCTGCTGA CAGCGGTCGT GGGAGCTGGA CATCATGCTC AAGTGGTTCC
    CATGACAACA TACAGACCAT TCAGCACCAG AGAAGCTGGG AAACACTGCC ATTTGGGCAC
    ACTCACTTTG ACTATTCAGG GGATGCTGCA AGTATATGGG CCTCCGGTGG CCATATGGAC
    CAAATGATGT TTTCTGATCA TAGCACAAAA TATAACAGAC AGAATCAAAG TAGAGAGAGT
    CTTGAACAAG CCCAATCCCG GGCCAGCTGG GCGTCTTCCA CGGGCTACTG GGGAGAAGAC
    TCAGAAGGCG ACACAGGCAC TATCAAGCGA AGGGGTGGGA AGGATGTCTC CGCTGAGGCA
    GAGAGCAGCA GCATGGTGCC CGTGACTACA GAGGAAGCCA AACCTGTCCC TATGCCTGCC
    CACATAGCTG TGACGCCGAG CACTACCAAG GGACTCATCG CACGGAAGGA AGGCAGGTAC
    CGGGAGCCGC CTCCCACACC TCCAGGCTAC GTGGGCATCC CCATTGCCGA TTTCCCAGAA
    GGGCCTTGCC ACCCGGCCAG GAAGCCCCCG GATTACAACG TGGCCCTGCA GCGGTCCCGC
    ATGGTGGCAC GGCCCACTGA GGCCCCGGCA CCGGGCCAGA CGCCGCCTGC AGCCGCAGCC
    AGCCGGCCGG GCAGCAAGCC ACAGTGGCAC AAGCCCAGCG ACGCAGACCC ACGCCTCGCG
    CCCTTCCAGC CGCAGGGCTT CGCAGGAGCG GAGGAGGACG AAGATGAACA AGTGTCTGCT
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006502315.1
    CDS14587..19212
    Translation

    Target ORF information:

    RefSeq Version XM_006502252.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006502252.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TCCAACGTCA AGCCTCCCAC 
    AGACAGTCAC GGAGAAGATT TAGAAAGATC AACCAGAAAG GTGAAAGACA AACAATTATC
    GACACAGTGG ACCCTTACCC TGTGGGCAAG CCACCTTTAC CTAGAGGCTA CCATACGGAA
    TGCACTAAAT CTCAGCTCCC TGCAGACTTC ACAAAACTGC ACCTCACTGA CAGCCTGCAC
    CCACAGGTGA CCCACGTGTC CTCCAGCCAT TCAGGATGTA GCATCACCAG CGACTCTGGG
    AGCAGCAGTC TTTCTGACAT TTACCAGGCC ACGGAGAGCG AGGCCGGTGA CATGGACCTG
    AGTGGACTGC CAGAGACGGC GGTTGACTCT GAGGATGACG ATGATGAGGA AGACATCGAG
    AGAGCATCAG ACCCTCTGAT GAGCAGGGAC ATCGTGAGGG ACTGCCTGGA GAAGGACCCG
    ATAGACAGGA CTGACGATGA CATCGAACAA CTACTGGAAT TTATGCACCA GCTGCCGGCC
    TTTGCCAATA TGACGATGTC AGTAAGACGG GAGCTCTGTG CCGTGATGGT GTTTGCAGTG
    GTGGAAAGAG CTGGAACCAT TGTGTTAAAC GACGGAGAAG AGCTGGATTC CTGGTCAGTG
    ATTCTCAATG GTTCCGTGGA GGTGACATAT CCGGATGGGA AAGCAGAAAT TCTGTGCATG
    GGAAACAGCT TTGGTGTTTC GCCCACCATG GACAAAGAAT ACATGAAAGG AGTCATGAGG
    ACTAAGGTGG ATGACTGCCA GTTTGTCTGC ATTGCCCAGC AAGATTACTG CCGTATTTTG
    AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAGGAGGAAG GAGAGATTGT AATGGTGAAG
    GAGCACCGAG AACTTGATCG GACTGGAACA AGGAAGGGCC ACATCGTCAT CAAGGGCACC
    TCAGAGAGGC TGACCATGCA TTTGGTGGAA GAGCATTCTG TAGTGGATCC AACATTCATA
    GAAGACTTCC TGTTGACATA CCGGACTTTC CTTTCCAGCC CGATGGAAGT GGGCAAGAAG
    TTATTGGAGT GGTTCAATGA CCCGAGCCTC AGGGATAAGG TGACGCGGGT AGTATTACTG
    TGGGTGAATA ATCACTTCAA TGACTTTGAA GGGGATCCTG CAATGACTCG ATTTCTGGAA
    GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTGAACATT
    GCATGTGCAG CTAAAGCCAA ACGAAGACTG ATGACGTTAA CAAAGCCTTC CCGGGAAGCC
    CCTCTGCCCT TCATCTTACT GGGAGGCTCT GAAAAGGGCT TTGGCATCTT CGTGGACAGC
    GTTGACTCGT GTAGCAAAGC CACTGAAGCA GGCTTGAAGC GCGGGGATCA GATATTAGAA
    GTGAATGGTC AAAATTTTGA AAATATCCAA CTTTCAAAGG CAATGGAAAT TCTCAGAAAT
    AACACACACC TGTCCATCAC TGTGAAAACC AACTTATTTG TATTTAAAGA ACTTCTGACG
    AGATTGTCTG AAGAGAAAAG AAACGGTGCC CCTCACCTCC CTAAAATCGG GGACATCAAG
    AAGGCCAGCC GCTACTCCAT ACCGGATCTC GCCGTAGACG TAGAACAGGT GATAGGCCTC
    GAGAAGGTCA ACAAGAAAAG CAAAGCCAAC ACCGTGGGAG GAAGAAACAA GCTGAAGAAA
    ATACTCGACA AGACCCGGAT CAGCATCTTA CCTCAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGATAG CATCGTAGGA CTGAGGCAGA CCAAGCACAT CCCAGCTGCC
    CTGCCTGTCA GTGGAACGCT GTCCTCCAGT AACCCTGACT TGCTGCAGTC ACACCACCGG
    ATTCTGGACT TCAGCACTAC TCCTGACTTG CCAGATCAGG TACTGAGAGT TTTCAAGGCT
    GACCAACAAA GCAGGTACAT CATGATCAGC AAGGACACCA CCGCAAAGGA AGTGGTCATC
    CAGGCCATCA GAGAGTTTGC TGTCACTGCC ACCCCAGAGC AGTATTCCCT CTGTGAGGTC
    TCAGTCACAC CAGAGGGAGT TATCAAACAA CGCCGACTCC CAGATCAGCT TTCCAAGCTT
    GCCGACAGGA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACG
    CTTTGCTCAG ATGAAGATGC CCAGGAGCTG CTGCGAGAGA GCCAAATCTC CCTCCTTCAG
    CTCAGCACCG TGGAGGTTGC CACGCAGCTC TCCATGAGGA ACTTTGAACT CTTCCGCAAC
    ATTGAGCCTA CTGAATACAT AGATGATTTA TTTAAACTCA AATCAAAAAC CAGCTGTGCC
    AACCTGAAGA AATTTGAGGA AGTCATTAAC CAGGAAACAT TTTGGGTGGC TTCTGAAATC
    CTGCGGGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATTGCC
    TTGCACTGCA GGGAATGCAA GAATTTTAAC TCCATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCGG TGGCAAGACT ACGAACCACC TGGGAAAAGC TTCCCAATAA ATATGAAAAG
    CTGTTCCAGG ACCTGCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA GTACCGCAAT
    GTCCTGAGTG GCCAGAACTT ACAGCCTCCT GTCATTCCCC TGTTCCCTGT CATCAAGAAG
    GACCTCACGT TTCTCCATGA GGGAAATGAC TCAAAAGTAG ACGGACTGGT CAACTTTGAG
    AAGCTGAGGA TGATTGCAAA AGAAATCCGT CACGTTGGCC GAATGGCATC AGTCAACATG
    GACCCTGCCC TTATGTTCAG GACTCGGAAG AAGAAGTGGC GGAGTCTGGG GTCCCTGAGT
    CAGGGCAGTG CAAACGCCAC AGTGCTGGAT GTTGCTCAGA CAGGCGGGCA TAAAAAGCGG
    GTACGCCGTA GTTCCTTCCT CAATGCCAAA AAGCTGTATG AAGATGCCCA GATGGCTCGG
    AAAGTGAAGC AATACCTGTC CAACCTGGAG CTGGAGATGG ATGAGGAGAG TCTTCAGACA
    CTGTCACTGC AGTGTGAACC AGCAACCAGC ACATTGCCCA AGAATCCTGG AGACAAAAAG
    CCTGTCAAGT CTGAGACCTC CCCAGTGGCT CCACGTGCAG GGCCCCAGCA GAAAGTACAG
    CCTCAGCAGC CGCTGGCACA GCCACAACCT CCACATAAAG TCAGCCAGGG ACTGCAGGTG
    CCCGCTGTGT CCCTCTATCC TTCACGAAAG AAGGTGCCGG TGAAGGATCT GCCTCCTTTT
    GGCATAAACT CTCCACAAGC TTTGAAGAAA ATCCTGTCCT TGTCTGAAGA AGGGAGTTTG
    GAGCGTCACA GGAAGCAAGC AGAAGATACA ATATCAAATG CGTCCTCTCA GCTCTCATCG
    CCCCCCACCT CACCTCAGAG CTCTCCAAGG AAAGGTTATG CACTGGCTCT CAGTGGCACC
    GTGGATAATT TCTCAGATTC AGGTCACAGT GAGATTTCTT CACGATCCAG TATTGTCAGC
    AATTCTTCTT TTGACTCGGT GCCAGTCTCT CTGCATGATG AACGGCGCCA GAGGCATTCT
    GTCAGCATTG TGGAGAGCAA CCTAGGTGTG GGCAGGATGG AGCGGCGGAC CCTGATGGAG
    CCTGATCAAT ACAGCTTAGG GTCGTATGCC CCAGTGTCTG AAAGCCGAGG CCTGTATGCC
    GCAGCGACGG TCATCTCTTC TCCTAGCACA GAGGAGCTTT CCCATGACCA AGGGGACCGT
    GCTTCCCTCG ATGCTGCTGA CAGCGGTCGT GGGAGCTGGA CATCATGCTC AAGTGGTTCC
    CATGACAACA TACAGACCAT TCAGCACCAG AGAAGCTGGG AAACACTGCC ATTTGGGCAC
    ACTCACTTTG ACTATTCAGG GGATGCTGCA AGTATATGGG CCTCCGGTGG CCATATGGAC
    CAAATGATGT TTTCTGATCA TAGCACAAAA TATAACAGAC AGAATCAAAG TAGAGAGAGT
    CTTGAACAAG CCCAATCCCG GGCCAGCTGG GCGTCTTCCA CGGGCTACTG GGGAGAAGAC
    TCAGAAGGCG ACACAGGCAC TATCAAGCGA AGGGGTGGGA AGGATGTCTC CGCTGAGGCA
    GAGAGCAGCA GCATGGTGCC CGTGACTACA GAGGAAGCCA AACCTGTCCC TATGCCTGCC
    CACATAGCTG TGACGCCGAG CACTACCAAG GGACTCATCG CACGGAAGGA AGGCAGGTAC
    CGGGAGCCGC CTCCCACACC TCCAGGCTAC GTGGGCATCC CCATTGCCGA TTTCCCAGAA
    GGGCCTTGCC ACCCGGCCAG GAAGCCCCCG GATTACAACG TGGCCCTGCA GCGGTCCCGC
    ATGGTGGCAC GGCCCACTGA GGCCCCGGCA CCGGGCCAGA CGCCGCCTGC AGCCGCAGCC
    AGCCGGCCGG GCAGCAAGCC ACAGTGGCAC AAGCCCAGCG ACGCAGACCC ACGCCTCGCG
    CCCTTCCAGC CGCAGGGCTT CGCAGGAGCG GAGGAGGACG AAGATGAACA AGTGTCTGCT
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu71469
    Clone ID Related Accession (Same CDS sequence) XM_011240276.3 , XM_011240276.2
    Accession Version XM_011240276.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240276.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGGAATG CACTAAATCT
    CAGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGAAGAA GAAGTGGCGG AGTCTGGGGT CCCTGAGTCA GGGCAGTGCA
    AACGCCACAG TGCTGGATGT TGCTCAGACA GGCGGGCATA AAAAGCGGGT ACGCCGTAGT
    TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGGAA AGTGAAGCAA
    TACCTGTCCA ACCTGGAGCT GGAGATGGAT GAGGAGAGTC TTCAGACACT GTCACTGCAG
    TGTGAACCAG CAACCAGCAC ATTGCCCAAG AATCCTGGAG ACAAAAAGCC TGTCAAGTCT
    GAGACCTCCC CAGTGGCTCC ACGTGCAGGG CCCCAGCAGA AAGTACAGCC TCAGCAGCCG
    CTGGCACAGC CACAACCTCC ACATAAAGTC AGCCAGGGAC TGCAGGTGCC CGCTGTGTCC
    CTCTATCCTT CACGAAAGAA GGTGCCGGTG AAGGATCTGC CTCCTTTTGG CATAAACTCT
    CCACAAGCTT TGAAGAAAAT CCTGTCCTTG TCTGAAGAAG GGAGTTTGGA GCGTCACAGG
    AAGCAAGCAG AAGATACAAT ATCAAATGCG TCCTCTCAGC TCTCATCGCC CCCCACCTCA
    CCTCAGAGCT CTCCAAGGAA AGGTTATGCA CTGGCTCTCA GTGGCACCGT GGATAATTTC
    TCAGATTCAG GTCACAGTGA GATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCTTTT
    GACTCGGTGC CAGTCTCTCT GCATGATGAA CGGCGCCAGA GGCATTCTGT CAGCATTGTG
    GAGAGCAACC TAGGTGTGGG CAGGATGGAG CGGCGGACCC TGATGGAGCC TGATCAATAC
    AGCTTAGGGT CGTATGCCCC AGTGTCTGAA AGCCGAGGCC TGTATGCCGC AGCGACGGTC
    ATCTCTTCTC CTAGCACAGA GGAGCTTTCC CATGACCAAG GGGACCGTGC TTCCCTCGAT
    GCTGCTGACA GCGGTCGTGG GAGCTGGACA TCATGCTCAA GTGGTTCCCA TGACAACATA
    CAGACCATTC AGCACCAGAG AAGCTGGGAA ACACTGCCAT TTGGGCACAC TCACTTTGAC
    TATTCAGGGG ATGCTGCAAG TATATGGGCC TCCGGTGGCC ATATGGACCA AATGATGTTT
    TCTGATCATA GCACAAAATA TAACAGACAG AATCAAAGTA GAGAGAGTCT TGAACAAGCC
    CAATCCCGGG CCAGCTGGGC GTCTTCCACG GGCTACTGGG GAGAAGACTC AGAAGGCGAC
    ACAGGCACTA TCAAGCGAAG GGGTGGGAAG GATGTCTCCG CTGAGGCAGA GAGCAGCAGC
    ATGGTGCCCG TGACTACAGA GGAAGCCAAA CCTGTCCCTA TGCCTGCCCA CATAGCTGTG
    ACGCCGAGCA CTACCAAGGG ACTCATCGCA CGGAAGGAAG GCAGGTACCG GGAGCCGCCT
    CCCACACCTC CAGGCTACGT GGGCATCCCC ATTGCCGATT TCCCAGAAGG GCCTTGCCAC
    CCGGCCAGGA AGCCCCCGGA TTACAACGTG GCCCTGCAGC GGTCCCGCAT GGTGGCACGG
    CCCACTGAGG CCCCGGCACC GGGCCAGACG CCGCCTGCAG CCGCAGCCAG CCGGCCGGGC
    AGCAAGCCAC AGTGGCACAA GCCCAGCGAC GCAGACCCAC GCCTCGCGCC CTTCCAGCCG
    CAGGGCTTCG CAGGAGCGGA GGAGGACGAA GATGAACAAG TGTCTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011238578.1
    CDS14..4987
    Translation

    Target ORF information:

    RefSeq Version XM_011240276.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011240276.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGGAATG CACTAAATCT
    CAGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGAAGAA GAAGTGGCGG AGTCTGGGGT CCCTGAGTCA GGGCAGTGCA
    AACGCCACAG TGCTGGATGT TGCTCAGACA GGCGGGCATA AAAAGCGGGT ACGCCGTAGT
    TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGGAA AGTGAAGCAA
    TACCTGTCCA ACCTGGAGCT GGAGATGGAT GAGGAGAGTC TTCAGACACT GTCACTGCAG
    TGTGAACCAG CAACCAGCAC ATTGCCCAAG AATCCTGGAG ACAAAAAGCC TGTCAAGTCT
    GAGACCTCCC CAGTGGCTCC ACGTGCAGGG CCCCAGCAGA AAGTACAGCC TCAGCAGCCG
    CTGGCACAGC CACAACCTCC ACATAAAGTC AGCCAGGGAC TGCAGGTGCC CGCTGTGTCC
    CTCTATCCTT CACGAAAGAA GGTGCCGGTG AAGGATCTGC CTCCTTTTGG CATAAACTCT
    CCACAAGCTT TGAAGAAAAT CCTGTCCTTG TCTGAAGAAG GGAGTTTGGA GCGTCACAGG
    AAGCAAGCAG AAGATACAAT ATCAAATGCG TCCTCTCAGC TCTCATCGCC CCCCACCTCA
    CCTCAGAGCT CTCCAAGGAA AGGTTATGCA CTGGCTCTCA GTGGCACCGT GGATAATTTC
    TCAGATTCAG GTCACAGTGA GATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCTTTT
    GACTCGGTGC CAGTCTCTCT GCATGATGAA CGGCGCCAGA GGCATTCTGT CAGCATTGTG
    GAGAGCAACC TAGGTGTGGG CAGGATGGAG CGGCGGACCC TGATGGAGCC TGATCAATAC
    AGCTTAGGGT CGTATGCCCC AGTGTCTGAA AGCCGAGGCC TGTATGCCGC AGCGACGGTC
    ATCTCTTCTC CTAGCACAGA GGAGCTTTCC CATGACCAAG GGGACCGTGC TTCCCTCGAT
    GCTGCTGACA GCGGTCGTGG GAGCTGGACA TCATGCTCAA GTGGTTCCCA TGACAACATA
    CAGACCATTC AGCACCAGAG AAGCTGGGAA ACACTGCCAT TTGGGCACAC TCACTTTGAC
    TATTCAGGGG ATGCTGCAAG TATATGGGCC TCCGGTGGCC ATATGGACCA AATGATGTTT
    TCTGATCATA GCACAAAATA TAACAGACAG AATCAAAGTA GAGAGAGTCT TGAACAAGCC
    CAATCCCGGG CCAGCTGGGC GTCTTCCACG GGCTACTGGG GAGAAGACTC AGAAGGCGAC
    ACAGGCACTA TCAAGCGAAG GGGTGGGAAG GATGTCTCCG CTGAGGCAGA GAGCAGCAGC
    ATGGTGCCCG TGACTACAGA GGAAGCCAAA CCTGTCCCTA TGCCTGCCCA CATAGCTGTG
    ACGCCGAGCA CTACCAAGGG ACTCATCGCA CGGAAGGAAG GCAGGTACCG GGAGCCGCCT
    CCCACACCTC CAGGCTACGT GGGCATCCCC ATTGCCGATT TCCCAGAAGG GCCTTGCCAC
    CCGGCCAGGA AGCCCCCGGA TTACAACGTG GCCCTGCAGC GGTCCCGCAT GGTGGCACGG
    CCCACTGAGG CCCCGGCACC GGGCCAGACG CCGCCTGCAG CCGCAGCCAG CCGGCCGGGC
    AGCAAGCCAC AGTGGCACAA GCCCAGCGAC GCAGACCCAC GCCTCGCGCC CTTCCAGCCG
    CAGGGCTTCG CAGGAGCGGA GGAGGACGAA GATGAACAAG TGTCTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu71470
    Clone ID Related Accession (Same CDS sequence) XM_011240277.2 , XM_011240277.3
    Accession Version XM_011240277.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240277.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGGAATG CACTAAATCT
    CAGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGTCCCT GAGTCAGGGC AGTGCAAACG CCACAGTGCT GGATGTTGCT
    CAGACAGGCG GGCATAAAAA GCGGGTACGC CGTAGTTCCT TCCTCAATGC CAAAAAGCTG
    TATGAAGATG CCCAGATGGC TCGGAAAGTG AAGCAATACC TGTCCAACCT GGAGCTGGAG
    ATGGATGAGG AGAGTCTTCA GACACTGTCA CTGCAGTGTG AACCAGCAAC CAGCACATTG
    CCCAAGAATC CTGGAGACAA AAAGCCTGTC AAGTCTGAGA CCTCCCCAGT GGCTCCACGT
    GCAGGGCCCC AGCAGAAAGT ACAGCCTCAG CAGCCGCTGG CACAGCCACA ACCTCCACAT
    AAAGTCAGCC AGGGACTGCA GGTGCCCGCT GTGTCCCTCT ATCCTTCACG AAAGAAGGTG
    CCGGTGAAGG ATCTGCCTCC TTTTGGCATA AACTCTCCAC AAGCTTTGAA GAAAATCCTG
    TCCTTGTCTG AAGAAGGGAG TTTGGAGCGT CACAGGAAGC AAGCAGAAGA TACAATATCA
    AATGCGTCCT CTCAGCTCTC ATCGCCCCCC ACCTCACCTC AGAGCTCTCC AAGGAAAGGT
    TATGCACTGG CTCTCAGTGG CACCGTGGAT AATTTCTCAG ATTCAGGTCA CAGTGAGATT
    TCTTCACGAT CCAGTATTGT CAGCAATTCT TCTTTTGACT CGGTGCCAGT CTCTCTGCAT
    GATGAACGGC GCCAGAGGCA TTCTGTCAGC ATTGTGGAGA GCAACCTAGG TGTGGGCAGG
    ATGGAGCGGC GGACCCTGAT GGAGCCTGAT CAATACAGCT TAGGGTCGTA TGCCCCAGTG
    TCTGAAAGCC GAGGCCTGTA TGCCGCAGCG ACGGTCATCT CTTCTCCTAG CACAGAGGAG
    CTTTCCCATG ACCAAGGGGA CCGTGCTTCC CTCGATGCTG CTGACAGCGG TCGTGGGAGC
    TGGACATCAT GCTCAAGTGG TTCCCATGAC AACATACAGA CCATTCAGCA CCAGAGAAGC
    TGGGAAACAC TGCCATTTGG GCACACTCAC TTTGACTATT CAGGGGATGC TGCAAGTATA
    TGGGCCTCCG GTGGCCATAT GGACCAAATG ATGTTTTCTG ATCATAGCAC AAAATATAAC
    AGACAGAATC AAAGTAGAGA GAGTCTTGAA CAAGCCCAAT CCCGGGCCAG CTGGGCGTCT
    TCCACGGGCT ACTGGGGAGA AGACTCAGAA GGCGACACAG GCACTATCAA GCGAAGGGGT
    GGGAAGGATG TCTCCGCTGA GGCAGAGAGC AGCAGCATGG TGCCCGTGAC TACAGAGGAA
    GCCAAACCTG TCCCTATGCC TGCCCACATA GCTGTGACGC CGAGCACTAC CAAGGGACTC
    ATCGCACGGA AGGAAGGCAG GTACCGGGAG CCGCCTCCCA CACCTCCAGG CTACGTGGGC
    ATCCCCATTG CCGATTTCCC AGAAGGGCCT TGCCACCCGG CCAGGAAGCC CCCGGATTAC
    AACGTGGCCC TGCAGCGGTC CCGCATGGTG GCACGGCCCA CTGAGGCCCC GGCACCGGGC
    CAGACGCCGC CTGCAGCCGC AGCCAGCCGG CCGGGCAGCA AGCCACAGTG GCACAAGCCC
    AGCGACGCAG ACCCACGCCT CGCGCCCTTC CAGCCGCAGG GCTTCGCAGG AGCGGAGGAG
    GACGAAGATG AACAAGTGTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011238579.1
    CDS14..4963
    Translation

    Target ORF information:

    RefSeq Version XM_011240277.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011240277.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGGAATG CACTAAATCT
    CAGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGTCCCT GAGTCAGGGC AGTGCAAACG CCACAGTGCT GGATGTTGCT
    CAGACAGGCG GGCATAAAAA GCGGGTACGC CGTAGTTCCT TCCTCAATGC CAAAAAGCTG
    TATGAAGATG CCCAGATGGC TCGGAAAGTG AAGCAATACC TGTCCAACCT GGAGCTGGAG
    ATGGATGAGG AGAGTCTTCA GACACTGTCA CTGCAGTGTG AACCAGCAAC CAGCACATTG
    CCCAAGAATC CTGGAGACAA AAAGCCTGTC AAGTCTGAGA CCTCCCCAGT GGCTCCACGT
    GCAGGGCCCC AGCAGAAAGT ACAGCCTCAG CAGCCGCTGG CACAGCCACA ACCTCCACAT
    AAAGTCAGCC AGGGACTGCA GGTGCCCGCT GTGTCCCTCT ATCCTTCACG AAAGAAGGTG
    CCGGTGAAGG ATCTGCCTCC TTTTGGCATA AACTCTCCAC AAGCTTTGAA GAAAATCCTG
    TCCTTGTCTG AAGAAGGGAG TTTGGAGCGT CACAGGAAGC AAGCAGAAGA TACAATATCA
    AATGCGTCCT CTCAGCTCTC ATCGCCCCCC ACCTCACCTC AGAGCTCTCC AAGGAAAGGT
    TATGCACTGG CTCTCAGTGG CACCGTGGAT AATTTCTCAG ATTCAGGTCA CAGTGAGATT
    TCTTCACGAT CCAGTATTGT CAGCAATTCT TCTTTTGACT CGGTGCCAGT CTCTCTGCAT
    GATGAACGGC GCCAGAGGCA TTCTGTCAGC ATTGTGGAGA GCAACCTAGG TGTGGGCAGG
    ATGGAGCGGC GGACCCTGAT GGAGCCTGAT CAATACAGCT TAGGGTCGTA TGCCCCAGTG
    TCTGAAAGCC GAGGCCTGTA TGCCGCAGCG ACGGTCATCT CTTCTCCTAG CACAGAGGAG
    CTTTCCCATG ACCAAGGGGA CCGTGCTTCC CTCGATGCTG CTGACAGCGG TCGTGGGAGC
    TGGACATCAT GCTCAAGTGG TTCCCATGAC AACATACAGA CCATTCAGCA CCAGAGAAGC
    TGGGAAACAC TGCCATTTGG GCACACTCAC TTTGACTATT CAGGGGATGC TGCAAGTATA
    TGGGCCTCCG GTGGCCATAT GGACCAAATG ATGTTTTCTG ATCATAGCAC AAAATATAAC
    AGACAGAATC AAAGTAGAGA GAGTCTTGAA CAAGCCCAAT CCCGGGCCAG CTGGGCGTCT
    TCCACGGGCT ACTGGGGAGA AGACTCAGAA GGCGACACAG GCACTATCAA GCGAAGGGGT
    GGGAAGGATG TCTCCGCTGA GGCAGAGAGC AGCAGCATGG TGCCCGTGAC TACAGAGGAA
    GCCAAACCTG TCCCTATGCC TGCCCACATA GCTGTGACGC CGAGCACTAC CAAGGGACTC
    ATCGCACGGA AGGAAGGCAG GTACCGGGAG CCGCCTCCCA CACCTCCAGG CTACGTGGGC
    ATCCCCATTG CCGATTTCCC AGAAGGGCCT TGCCACCCGG CCAGGAAGCC CCCGGATTAC
    AACGTGGCCC TGCAGCGGTC CCGCATGGTG GCACGGCCCA CTGAGGCCCC GGCACCGGGC
    CAGACGCCGC CTGCAGCCGC AGCCAGCCGG CCGGGCAGCA AGCCACAGTG GCACAAGCCC
    AGCGACGCAG ACCCACGCCT CGCGCCCTTC CAGCCGCAGG GCTTCGCAGG AGCGGAGGAG
    GACGAAGATG AACAAGTGTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21032
    Clone ID Related Accession (Same CDS sequence) NM_001099624.3
    Accession Version NM_001099624.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC067056.1, AK147368.1 and BP758717.1. On Jul 15, 2015 this sequence version replaced NM_001099624.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC067056.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCCTC CAAACTCCCT 
    GCAGACTTCA CAAAACTGCA CCTCACTGAC AGCCTGCACC CACAGGTGAC CCACGTGTCC
    TCCAGCCATT CAGGATGTAG CATCACCAGC GACTCTGGGA GCAGCAGTCT TTCTGACATT
    TACCAGGCCA CGGAGAGCGA GGCCGGTGAC ATGGACCTGA GTGGACTGCC AGAGACGGCG
    GTTGACTCTG AGGATGACGA TGATGAGGAA GACATCGAGA GAGCATCAGA CCCTCTGATG
    AGCAGGGACA TCGTGAGGGA CTGCCTGGAG AAGGACCCGA TAGACAGGAC TGACGATGAC
    ATCGAACAAC TACTGGAATT TATGCACCAG CTGCCGGCCT TTGCCAATAT GACGATGTCA
    GTAAGACGGG AGCTCTGTGC CGTGATGGTG TTTGCAGTGG TGGAAAGAGC TGGAACCATT
    GTGTTAAACG ACGGAGAAGA GCTGGATTCC TGGTCAGTGA TTCTCAATGG TTCCGTGGAG
    GTGACATATC CGGATGGGAA AGCAGAAATT CTGTGCATGG GAAACAGCTT TGGTGTTTCG
    CCCACCATGG ACAAAGAATA CATGAAAGGA GTCATGAGGA CTAAGGTGGA TGACTGCCAG
    TTTGTCTGCA TTGCCCAGCA AGATTACTGC CGTATTTTGA ACCAAGTAGA AAAGAACATG
    CAAAAAGTTG AGGAGGAAGG AGAGATTGTA ATGGTGAAGG AGCACCGAGA ACTTGATCGG
    ACTGGAACAA GGAAGGGCCA CATCGTCATC AAGGGCACCT CAGAGAGGCT GACCATGCAT
    TTGGTGGAAG AGCATTCTGT AGTGGATCCA ACATTCATAG AAGACTTCCT GTTGACATAC
    CGGACTTTCC TTTCCAGCCC GATGGAAGTG GGCAAGAAGT TATTGGAGTG GTTCAATGAC
    CCGAGCCTCA GGGATAAGGT GACGCGGGTA GTATTACTGT GGGTGAATAA TCACTTCAAT
    GACTTTGAAG GGGATCCTGC AATGACTCGA TTTCTGGAAG AATTTGAAAA TAATCTGGAA
    AGAGAGAAAA TGGGTGGACA TCTAAGGCTG TTGAACATTG CATGTGCAGC TAAAGCCAAA
    CGAAGACTGA TGACGTTAAC AAAGCCTTCC CGGGAAGCCC CTCTGCCCTT CATCTTACTG
    GGAGGCTCTG AAAAGGGCTT TGGCATCTTC GTGGACAGCG TTGACTCGTG TAGCAAAGCC
    ACTGAAGCAG GCTTGAAGCG CGGGGATCAG ATATTAGAAG TGAATGGTCA AAATTTTGAA
    AATATCCAAC TTTCAAAGGC AATGGAAATT CTCAGAAATA ACACACACCT GTCCATCACT
    GTGAAAACCA ACTTATTTGT ATTTAAAGAA CTTCTGACGA GATTGTCTGA AGAGAAAAGA
    AACGGTGCCC CTCACCTCCC TAAAATCGGG GACATCAAGA AGGCCAGCCG CTACTCCATA
    CCGGATCTCG CCGTAGACGT AGAACAGGTG ATAGGCCTCG AGAAGGTCAA CAAGAAAAGC
    AAAGCCAACA CCGTGGGAGG AAGAAACAAG CTGAAGAAAA TACTCGACAA GACCCGGATC
    AGCATCTTAC CTCAGAAACC ATACAATGAT ATTGGGATTG GTCAGTCTCA AGATGATAGC
    ATCGTAGGAC TGAGGCAGAC CAAGCACATC CCAGCTGCCC TGCCTGTCAG TGGAACGCTG
    TCCTCCAGTA ACCCTGACTT GCTGCAGTCA CACCACCGGA TTCTGGACTT CAGCACTACT
    CCTGACTTGC CAGATCAGGT ACTGAGAGTT TTCAAGGCTG ACCAACAAAG CAGGTACATC
    ATGATCAGCA AGGACACCAC CGCAAAGGAA GTGGTCATCC AGGCCATCAG AGAGTTTGCT
    GTCACTGCCA CCCCAGAGCA GTATTCCCTC TGTGAGGTCT CAGTCACACC AGAGGGAGTT
    ATCAAACAAC GCCGACTCCC AGATCAGCTT TCCAAGCTTG CCGACAGGAT ACAACTGAGT
    GGAAGGTATT ATCTGAAAAA CAACATGGAA ACAGAAACGC TTTGCTCAGA TGAAGATGCC
    CAGGAGCTGC TGCGAGAGAG CCAAATCTCC CTCCTTCAGC TCAGCACCGT GGAGGTTGCC
    ACGCAGCTCT CCATGAGGAA CTTTGAACTC TTCCGCAACA TTGAGCCTAC TGAATACATA
    GATGATTTAT TTAAACTCAA ATCAAAAACC AGCTGTGCCA ACCTGAAGAA ATTTGAGGAA
    GTCATTAACC AGGAAACATT TTGGGTGGCT TCTGAAATCC TGCGGGAAAC AAACCAGCTG
    AAGAGGATGA AGATCATTAA GCATTTCATC AAGATTGCCT TGCACTGCAG GGAATGCAAG
    AATTTTAACT CCATGTTTGC AATCATCAGT GGCCTAAACC TGGCACCGGT GGCAAGACTA
    CGAACCACCT GGGAAAAGCT TCCCAATAAA TATGAAAAGC TGTTCCAGGA CCTGCAAGAC
    CTGTTTGATC CTTCCAGAAA CATGGCAAAG TACCGCAATG TCCTGAGTGG CCAGAACTTA
    CAGCCTCCTG TCATTCCCCT GTTCCCTGTC ATCAAGAAGG ACCTCACGTT TCTCCATGAG
    GGAAATGACT CAAAAGTAGA CGGACTGGTC AACTTTGAGA AGCTGAGGAT GATTGCAAAA
    GAAATCCGTC ACGTTGGCCG AATGGCATCA GTCAACATGG ACCCTGCCCT TATGTTCAGG
    ACTCGGAAGA AGAAGTGGCG GAGTCTGGGG TCCCTGAGTC AGGGCAGTGC AAACGCCACA
    GTGCTGGATG TTGCTCAGAC AGGCGGGCAT AAAAAGCGGG TACGCCGTAG TTCCTTCCTC
    AATGCCAAAA AGCTGTATGA AGATGCCCAG ATGGCTCGGA AAGTGAAGCA ATACCTGTCC
    AACCTGGAGC TGGAGATGGA TGAGGAGAGT CTTCAGACAC TGTCACTGCA GTGTGAACCA
    GCAACCAGCA CATTGCCCAA GAATCCTGGA GACAAAAAGC CTGTCAAGTC TGAGACCTCC
    CCAGTGGCTC CACGTGCAGG GCCCCAGCAG AAAGTACAGC CTCAGCAGCC GCTGGCACAG
    CCACAACCTC CACATAAAGT CAGCCAGGGA CTGCAGGTGC CCGCTGTGTC CCTCTATCCT
    TCACGAAAGA AGGTGCCGGT GAAGGATCTG CCTCCTTTTG GCATAAACTC TCCACAAGCT
    TTGAAGAAAA TCCTGTCCTT GTCTGAAGAA GGGAGTTTGG AGCGTCACAG GAAGCAAGCA
    GAAGATACAA TATCAAATGC GTCCTCTCAG CTCTCATCGC CCCCCACCTC ACCTCAGAGC
    TCTCCAAGGA AAGGTTATGC ACTGGCTCTC AGTGGCACCG TGGATAATTT CTCAGATTCA
    GGTCACAGTG AGATTTCTTC ACGATCCAGT ATTGTCAGCA ATTCTTCTTT TGACTCGGTG
    CCAGTCTCTC TGCATGATGA ACGGCGCCAG AGGCATTCTG TCAGCATTGT GGAGAGCAAC
    CTAGGTGTGG GCAGGATGGA GCGGCGGACC CTGATGGAGC CTGATCAATA CAGCTTAGGG
    TCGTATGCCC CAGTGTCTGA AAGCCGAGGC CTGTATGCCG CAGCGACGGT CATCTCTTCT
    CCTAGCACAG AGGAGCTTTC CCATGACCAA GGGGACCGTG CTTCCCTCGA TGCTGCTGAC
    AGCGGTCGTG GGAGCTGGAC ATCATGCTCA AGTGGTTCCC ATGACAACAT ACAGACCATT
    CAGCACCAGA GAAGCTGGGA AACACTGCCA TTTGGGCACA CTCACTTTGA CTATTCAGGG
    GATGCTGCAA GTATATGGGC CTCCGGTGGC CATATGGACC AAATGATGTT TTCTGATCAT
    AGCACAAAAT ATAACAGACA GAATCAAAGT AGAGAGAGTC TTGAACAAGC CCAATCCCGG
    GCCAGCTGGG CGTCTTCCAC GGGCTACTGG GGAGAAGACT CAGAAGGCGA CACAGGCACT
    ATCAAGCGAA GGGGTGGGAA GGATGTCTCC GCTGAGGCAG AGAGCAGCAG CATGGTGCCC
    GTGACTACAG AGGAAGCCAA ACCTGTCCCT ATGCCTGCCC ACATAGCTGT GACGCCGAGC
    ACTACCAAGG GACTCATCGC ACGGAAGGAA GGCAGGTACC GGGAGCCGCC TCCCACACCT
    CCAGGCTACG TGGGCATCCC CATTGCCGAT TTCCCAGAAG GGCCTTGCCA CCCGGCCAGG
    AAGCCCCCGG ATTACAACGT GGCCCTGCAG CGGTCCCGCA TGGTGGCACG GCCCACTGAG
    GCCCCGGCAC CGGGCCAGAC GCCGCCTGCA GCCGCAGCCA GCCGGCCGGG CAGCAAGCCA
    CAGTGGCACA AGCCCAGCGA CGCAGACCCA CGCCTCGCGC CCTTCCAGCC GCAGGGCTTC
    GCAGGAGCGG AGGAGGACGA AGATGAACAA GTGTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001093094.2
    CDS52..4536
    Translation

    Target ORF information:

    RefSeq Version NM_001099624.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001099624.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCCTC CAAACTCCCT 
    GCAGACTTCA CAAAACTGCA CCTCACTGAC AGCCTGCACC CACAGGTGAC CCACGTGTCC
    TCCAGCCATT CAGGATGTAG CATCACCAGC GACTCTGGGA GCAGCAGTCT TTCTGACATT
    TACCAGGCCA CGGAGAGCGA GGCCGGTGAC ATGGACCTGA GTGGACTGCC AGAGACGGCG
    GTTGACTCTG AGGATGACGA TGATGAGGAA GACATCGAGA GAGCATCAGA CCCTCTGATG
    AGCAGGGACA TCGTGAGGGA CTGCCTGGAG AAGGACCCGA TAGACAGGAC TGACGATGAC
    ATCGAACAAC TACTGGAATT TATGCACCAG CTGCCGGCCT TTGCCAATAT GACGATGTCA
    GTAAGACGGG AGCTCTGTGC CGTGATGGTG TTTGCAGTGG TGGAAAGAGC TGGAACCATT
    GTGTTAAACG ACGGAGAAGA GCTGGATTCC TGGTCAGTGA TTCTCAATGG TTCCGTGGAG
    GTGACATATC CGGATGGGAA AGCAGAAATT CTGTGCATGG GAAACAGCTT TGGTGTTTCG
    CCCACCATGG ACAAAGAATA CATGAAAGGA GTCATGAGGA CTAAGGTGGA TGACTGCCAG
    TTTGTCTGCA TTGCCCAGCA AGATTACTGC CGTATTTTGA ACCAAGTAGA AAAGAACATG
    CAAAAAGTTG AGGAGGAAGG AGAGATTGTA ATGGTGAAGG AGCACCGAGA ACTTGATCGG
    ACTGGAACAA GGAAGGGCCA CATCGTCATC AAGGGCACCT CAGAGAGGCT GACCATGCAT
    TTGGTGGAAG AGCATTCTGT AGTGGATCCA ACATTCATAG AAGACTTCCT GTTGACATAC
    CGGACTTTCC TTTCCAGCCC GATGGAAGTG GGCAAGAAGT TATTGGAGTG GTTCAATGAC
    CCGAGCCTCA GGGATAAGGT GACGCGGGTA GTATTACTGT GGGTGAATAA TCACTTCAAT
    GACTTTGAAG GGGATCCTGC AATGACTCGA TTTCTGGAAG AATTTGAAAA TAATCTGGAA
    AGAGAGAAAA TGGGTGGACA TCTAAGGCTG TTGAACATTG CATGTGCAGC TAAAGCCAAA
    CGAAGACTGA TGACGTTAAC AAAGCCTTCC CGGGAAGCCC CTCTGCCCTT CATCTTACTG
    GGAGGCTCTG AAAAGGGCTT TGGCATCTTC GTGGACAGCG TTGACTCGTG TAGCAAAGCC
    ACTGAAGCAG GCTTGAAGCG CGGGGATCAG ATATTAGAAG TGAATGGTCA AAATTTTGAA
    AATATCCAAC TTTCAAAGGC AATGGAAATT CTCAGAAATA ACACACACCT GTCCATCACT
    GTGAAAACCA ACTTATTTGT ATTTAAAGAA CTTCTGACGA GATTGTCTGA AGAGAAAAGA
    AACGGTGCCC CTCACCTCCC TAAAATCGGG GACATCAAGA AGGCCAGCCG CTACTCCATA
    CCGGATCTCG CCGTAGACGT AGAACAGGTG ATAGGCCTCG AGAAGGTCAA CAAGAAAAGC
    AAAGCCAACA CCGTGGGAGG AAGAAACAAG CTGAAGAAAA TACTCGACAA GACCCGGATC
    AGCATCTTAC CTCAGAAACC ATACAATGAT ATTGGGATTG GTCAGTCTCA AGATGATAGC
    ATCGTAGGAC TGAGGCAGAC CAAGCACATC CCAGCTGCCC TGCCTGTCAG TGGAACGCTG
    TCCTCCAGTA ACCCTGACTT GCTGCAGTCA CACCACCGGA TTCTGGACTT CAGCACTACT
    CCTGACTTGC CAGATCAGGT ACTGAGAGTT TTCAAGGCTG ACCAACAAAG CAGGTACATC
    ATGATCAGCA AGGACACCAC CGCAAAGGAA GTGGTCATCC AGGCCATCAG AGAGTTTGCT
    GTCACTGCCA CCCCAGAGCA GTATTCCCTC TGTGAGGTCT CAGTCACACC AGAGGGAGTT
    ATCAAACAAC GCCGACTCCC AGATCAGCTT TCCAAGCTTG CCGACAGGAT ACAACTGAGT
    GGAAGGTATT ATCTGAAAAA CAACATGGAA ACAGAAACGC TTTGCTCAGA TGAAGATGCC
    CAGGAGCTGC TGCGAGAGAG CCAAATCTCC CTCCTTCAGC TCAGCACCGT GGAGGTTGCC
    ACGCAGCTCT CCATGAGGAA CTTTGAACTC TTCCGCAACA TTGAGCCTAC TGAATACATA
    GATGATTTAT TTAAACTCAA ATCAAAAACC AGCTGTGCCA ACCTGAAGAA ATTTGAGGAA
    GTCATTAACC AGGAAACATT TTGGGTGGCT TCTGAAATCC TGCGGGAAAC AAACCAGCTG
    AAGAGGATGA AGATCATTAA GCATTTCATC AAGATTGCCT TGCACTGCAG GGAATGCAAG
    AATTTTAACT CCATGTTTGC AATCATCAGT GGCCTAAACC TGGCACCGGT GGCAAGACTA
    CGAACCACCT GGGAAAAGCT TCCCAATAAA TATGAAAAGC TGTTCCAGGA CCTGCAAGAC
    CTGTTTGATC CTTCCAGAAA CATGGCAAAG TACCGCAATG TCCTGAGTGG CCAGAACTTA
    CAGCCTCCTG TCATTCCCCT GTTCCCTGTC ATCAAGAAGG ACCTCACGTT TCTCCATGAG
    GGAAATGACT CAAAAGTAGA CGGACTGGTC AACTTTGAGA AGCTGAGGAT GATTGCAAAA
    GAAATCCGTC ACGTTGGCCG AATGGCATCA GTCAACATGG ACCCTGCCCT TATGTTCAGG
    ACTCGGAAGA AGAAGTGGCG GAGTCTGGGG TCCCTGAGTC AGGGCAGTGC AAACGCCACA
    GTGCTGGATG TTGCTCAGAC AGGCGGGCAT AAAAAGCGGG TACGCCGTAG TTCCTTCCTC
    AATGCCAAAA AGCTGTATGA AGATGCCCAG ATGGCTCGGA AAGTGAAGCA ATACCTGTCC
    AACCTGGAGC TGGAGATGGA TGAGGAGAGT CTTCAGACAC TGTCACTGCA GTGTGAACCA
    GCAACCAGCA CATTGCCCAA GAATCCTGGA GACAAAAAGC CTGTCAAGTC TGAGACCTCC
    CCAGTGGCTC CACGTGCAGG GCCCCAGCAG AAAGTACAGC CTCAGCAGCC GCTGGCACAG
    CCACAACCTC CACATAAAGT CAGCCAGGGA CTGCAGGTGC CCGCTGTGTC CCTCTATCCT
    TCACGAAAGA AGGTGCCGGT GAAGGATCTG CCTCCTTTTG GCATAAACTC TCCACAAGCT
    TTGAAGAAAA TCCTGTCCTT GTCTGAAGAA GGGAGTTTGG AGCGTCACAG GAAGCAAGCA
    GAAGATACAA TATCAAATGC GTCCTCTCAG CTCTCATCGC CCCCCACCTC ACCTCAGAGC
    TCTCCAAGGA AAGGTTATGC ACTGGCTCTC AGTGGCACCG TGGATAATTT CTCAGATTCA
    GGTCACAGTG AGATTTCTTC ACGATCCAGT ATTGTCAGCA ATTCTTCTTT TGACTCGGTG
    CCAGTCTCTC TGCATGATGA ACGGCGCCAG AGGCATTCTG TCAGCATTGT GGAGAGCAAC
    CTAGGTGTGG GCAGGATGGA GCGGCGGACC CTGATGGAGC CTGATCAATA CAGCTTAGGG
    TCGTATGCCC CAGTGTCTGA AAGCCGAGGC CTGTATGCCG CAGCGACGGT CATCTCTTCT
    CCTAGCACAG AGGAGCTTTC CCATGACCAA GGGGACCGTG CTTCCCTCGA TGCTGCTGAC
    AGCGGTCGTG GGAGCTGGAC ATCATGCTCA AGTGGTTCCC ATGACAACAT ACAGACCATT
    CAGCACCAGA GAAGCTGGGA AACACTGCCA TTTGGGCACA CTCACTTTGA CTATTCAGGG
    GATGCTGCAA GTATATGGGC CTCCGGTGGC CATATGGACC AAATGATGTT TTCTGATCAT
    AGCACAAAAT ATAACAGACA GAATCAAAGT AGAGAGAGTC TTGAACAAGC CCAATCCCGG
    GCCAGCTGGG CGTCTTCCAC GGGCTACTGG GGAGAAGACT CAGAAGGCGA CACAGGCACT
    ATCAAGCGAA GGGGTGGGAA GGATGTCTCC GCTGAGGCAG AGAGCAGCAG CATGGTGCCC
    GTGACTACAG AGGAAGCCAA ACCTGTCCCT ATGCCTGCCC ACATAGCTGT GACGCCGAGC
    ACTACCAAGG GACTCATCGC ACGGAAGGAA GGCAGGTACC GGGAGCCGCC TCCCACACCT
    CCAGGCTACG TGGGCATCCC CATTGCCGAT TTCCCAGAAG GGCCTTGCCA CCCGGCCAGG
    AAGCCCCCGG ATTACAACGT GGCCCTGCAG CGGTCCCGCA TGGTGGCACG GCCCACTGAG
    GCCCCGGCAC CGGGCCAGAC GCCGCCTGCA GCCGCAGCCA GCCGGCCGGG CAGCAAGCCA
    CAGTGGCACA AGCCCAGCGA CGCAGACCCA CGCCTCGCGC CCTTCCAGCC GCAGGGCTTC
    GCAGGAGCGG AGGAGGACGA AGATGAACAA GTGTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32400
    Clone ID Related Accession (Same CDS sequence) XM_006502254.3 , NM_001310536.2
    Accession Version NM_001310536.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124358.4 and AC164560.3. On or before Feb 12, 2019 this sequence version replaced XM_006502254.3, NM_001310536.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream in-frame start site compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147368.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TGGCCGCGCC TGCTAACCAT GGAGTTCTGG GCCAGCAGGA GAAGCAGTCT 
    CTCCCTGCAG ACTTCACAAA ACTGCACCTC ACTGACAGCC TGCACCCACA GGTGACCCAC
    GTGTCCTCCA GCCATTCAGG ATGTAGCATC ACCAGCGACT CTGGGAGCAG CAGTCTTTCT
    GACATTTACC AGGCCACGGA GAGCGAGGCC GGTGACATGG ACCTGAGTGG ACTGCCAGAG
    ACGGCGGTTG ACTCTGAGGA TGACGATGAT GAGGAAGACA TCGAGAGAGC ATCAGACCCT
    CTGATGAGCA GGGACATCGT GAGGGACTGC CTGGAGAAGG ACCCGATAGA CAGGACTGAC
    GATGACATCG AACAACTACT GGAATTTATG CACCAGCTGC CGGCCTTTGC CAATATGACG
    ATGTCAGTAA GACGGGAGCT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGA
    ACCATTGTGT TAAACGACGG AGAAGAGCTG GATTCCTGGT CAGTGATTCT CAATGGTTCC
    GTGGAGGTGA CATATCCGGA TGGGAAAGCA GAAATTCTGT GCATGGGAAA CAGCTTTGGT
    GTTTCGCCCA CCATGGACAA AGAATACATG AAAGGAGTCA TGAGGACTAA GGTGGATGAC
    TGCCAGTTTG TCTGCATTGC CCAGCAAGAT TACTGCCGTA TTTTGAACCA AGTAGAAAAG
    AACATGCAAA AAGTTGAGGA GGAAGGAGAG ATTGTAATGG TGAAGGAGCA CCGAGAACTT
    GATCGGACTG GAACAAGGAA GGGCCACATC GTCATCAAGG GCACCTCAGA GAGGCTGACC
    ATGCATTTGG TGGAAGAGCA TTCTGTAGTG GATCCAACAT TCATAGAAGA CTTCCTGTTG
    ACATACCGGA CTTTCCTTTC CAGCCCGATG GAAGTGGGCA AGAAGTTATT GGAGTGGTTC
    AATGACCCGA GCCTCAGGGA TAAGGTGACG CGGGTAGTAT TACTGTGGGT GAATAATCAC
    TTCAATGACT TTGAAGGGGA TCCTGCAATG ACTCGATTTC TGGAAGAATT TGAAAATAAT
    CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTGA ACATTGCATG TGCAGCTAAA
    GCCAAACGAA GACTGATGAC GTTAACAAAG CCTTCCCGGG AAGCCCCTCT GCCCTTCATC
    TTACTGGGAG GCTCTGAAAA GGGCTTTGGC ATCTTCGTGG ACAGCGTTGA CTCGTGTAGC
    AAAGCCACTG AAGCAGGCTT GAAGCGCGGG GATCAGATAT TAGAAGTGAA TGGTCAAAAT
    TTTGAAAATA TCCAACTTTC AAAGGCAATG GAAATTCTCA GAAATAACAC ACACCTGTCC
    ATCACTGTGA AAACCAACTT ATTTGTATTT AAAGAACTTC TGACGAGATT GTCTGAAGAG
    AAAAGAAACG GTGCCCCTCA CCTCCCTAAA ATCGGGGACA TCAAGAAGGC CAGCCGCTAC
    TCCATACCGG ATCTCGCCGT AGACGTAGAA CAGGTGATAG GCCTCGAGAA GGTCAACAAG
    AAAAGCAAAG CCAACACCGT GGGAGGAAGA AACAAGCTGA AGAAAATACT CGACAAGACC
    CGGATCAGCA TCTTACCTCA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GATAGCATCG TAGGACTGAG GCAGACCAAG CACATCCCAG CTGCCCTGCC TGTCAGTGGA
    ACGCTGTCCT CCAGTAACCC TGACTTGCTG CAGTCACACC ACCGGATTCT GGACTTCAGC
    ACTACTCCTG ACTTGCCAGA TCAGGTACTG AGAGTTTTCA AGGCTGACCA ACAAAGCAGG
    TACATCATGA TCAGCAAGGA CACCACCGCA AAGGAAGTGG TCATCCAGGC CATCAGAGAG
    TTTGCTGTCA CTGCCACCCC AGAGCAGTAT TCCCTCTGTG AGGTCTCAGT CACACCAGAG
    GGAGTTATCA AACAACGCCG ACTCCCAGAT CAGCTTTCCA AGCTTGCCGA CAGGATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACGCTTTG CTCAGATGAA
    GATGCCCAGG AGCTGCTGCG AGAGAGCCAA ATCTCCCTCC TTCAGCTCAG CACCGTGGAG
    GTTGCCACGC AGCTCTCCAT GAGGAACTTT GAACTCTTCC GCAACATTGA GCCTACTGAA
    TACATAGATG ATTTATTTAA ACTCAAATCA AAAACCAGCT GTGCCAACCT GAAGAAATTT
    GAGGAAGTCA TTAACCAGGA AACATTTTGG GTGGCTTCTG AAATCCTGCG GGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCCTTGCA CTGCAGGGAA
    TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCGGTGGCA
    AGACTACGAA CCACCTGGGA AAAGCTTCCC AATAAATATG AAAAGCTGTT CCAGGACCTG
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAGTACC GCAATGTCCT GAGTGGCCAG
    AACTTACAGC CTCCTGTCAT TCCCCTGTTC CCTGTCATCA AGAAGGACCT CACGTTTCTC
    CATGAGGGAA ATGACTCAAA AGTAGACGGA CTGGTCAACT TTGAGAAGCT GAGGATGATT
    GCAAAAGAAA TCCGTCACGT TGGCCGAATG GCATCAGTCA ACATGGACCC TGCCCTTATG
    TTCAGGACTC GGAAGAAGAA GTGGCGGAGT CTGGGGTCCC TGAGTCAGGG CAGTGCAAAC
    GCCACAGTGC TGGATGTTGC TCAGACAGGC GGGCATAAAA AGCGGGTACG CCGTAGTTCC
    TTCCTCAATG CCAAAAAGCT GTATGAAGAT GCCCAGATGG CTCGGAAAGT GAAGCAATAC
    CTGTCCAACC TGGAGCTGGA GATGGATGAG GAGAGTCTTC AGACACTGTC ACTGCAGTGT
    GAACCAGCAA CCAGCACATT GCCCAAGAAT CCTGGAGACA AAAAGCCTGT CAAGTCTGAG
    ACCTCCCCAG TGGCTCCACG TGCAGGGCCC CAGCAGAAAG TACAGCCTCA GCAGCCGCTG
    GCACAGCCAC AACCTCCACA TAAAGTCAGC CAGGGACTGC AGGTGCCCGC TGTGTCCCTC
    TATCCTTCAC GAAAGAAGGT GCCGGTGAAG GATCTGCCTC CTTTTGGCAT AAACTCTCCA
    CAAGCTTTGA AGAAAATCCT GTCCTTGTCT GAAGAAGGGA GTTTGGAGCG TCACAGGAAG
    CAAGCAGAAG ATACAATATC AAATGCGTCC TCTCAGCTCT CATCGCCCCC CACCTCACCT
    CAGAGCTCTC CAAGGAAAGG TTATGCACTG GCTCTCAGTG GCACCGTGGA TAATTTCTCA
    GATTCAGGTC ACAGTGAGAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCTTTTGAC
    TCGGTGCCAG TCTCTCTGCA TGATGAACGG CGCCAGAGGC ATTCTGTCAG CATTGTGGAG
    AGCAACCTAG GTGTGGGCAG GATGGAGCGG CGGACCCTGA TGGAGCCTGA TCAATACAGC
    TTAGGGTCGT ATGCCCCAGT GTCTGAAAGC CGAGGCCTGT ATGCCGCAGC GACGGTCATC
    TCTTCTCCTA GCACAGAGGA GCTTTCCCAT GACCAAGGGG ACCGTGCTTC CCTCGATGCT
    GCTGACAGCG GTCGTGGGAG CTGGACATCA TGCTCAAGTG GTTCCCATGA CAACATACAG
    ACCATTCAGC ACCAGAGAAG CTGGGAAACA CTGCCATTTG GGCACACTCA CTTTGACTAT
    TCAGGGGATG CTGCAAGTAT ATGGGCCTCC GGTGGCCATA TGGACCAAAT GATGTTTTCT
    GATCATAGCA CAAAATATAA CAGACAGAAT CAAAGTAGAG AGAGTCTTGA ACAAGCCCAA
    TCCCGGGCCA GCTGGGCGTC TTCCACGGGC TACTGGGGAG AAGACTCAGA AGGCGACACA
    GGCACTATCA AGCGAAGGGG TGGGAAGGAT GTCTCCGCTG AGGCAGAGAG CAGCAGCATG
    GTGCCCGTGA CTACAGAGGA AGCCAAACCT GTCCCTATGC CTGCCCACAT AGCTGTGACG
    CCGAGCACTA CCAAGGGACT CATCGCACGG AAGGAAGGCA GGTACCGGGA GCCGCCTCCC
    ACACCTCCAG GCTACGTGGG CATCCCCATT GCCGATTTCC CAGAAGGGCC TTGCCACCCG
    GCCAGGAAGC CCCCGGATTA CAACGTGGCC CTGCAGCGGT CCCGCATGGT GGCACGGCCC
    ACTGAGGCCC CGGCACCGGG CCAGACGCCG CCTGCAGCCG CAGCCAGCCG GCCGGGCAGC
    AAGCCACAGT GGCACAAGCC CAGCGACGCA GACCCACGCC TCGCGCCCTT CCAGCCGCAG
    GGCTTCGCAG GAGCGGAGGA GGACGAAGAT GAACAAGTGT CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001297465.2
    CDS832..5322
    Translation

    Target ORF information:

    RefSeq Version NM_001310536.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001310536.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TGGCCGCGCC TGCTAACCAT GGAGTTCTGG GCCAGCAGGA GAAGCAGTCT 
    CTCCCTGCAG ACTTCACAAA ACTGCACCTC ACTGACAGCC TGCACCCACA GGTGACCCAC
    GTGTCCTCCA GCCATTCAGG ATGTAGCATC ACCAGCGACT CTGGGAGCAG CAGTCTTTCT
    GACATTTACC AGGCCACGGA GAGCGAGGCC GGTGACATGG ACCTGAGTGG ACTGCCAGAG
    ACGGCGGTTG ACTCTGAGGA TGACGATGAT GAGGAAGACA TCGAGAGAGC ATCAGACCCT
    CTGATGAGCA GGGACATCGT GAGGGACTGC CTGGAGAAGG ACCCGATAGA CAGGACTGAC
    GATGACATCG AACAACTACT GGAATTTATG CACCAGCTGC CGGCCTTTGC CAATATGACG
    ATGTCAGTAA GACGGGAGCT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGA
    ACCATTGTGT TAAACGACGG AGAAGAGCTG GATTCCTGGT CAGTGATTCT CAATGGTTCC
    GTGGAGGTGA CATATCCGGA TGGGAAAGCA GAAATTCTGT GCATGGGAAA CAGCTTTGGT
    GTTTCGCCCA CCATGGACAA AGAATACATG AAAGGAGTCA TGAGGACTAA GGTGGATGAC
    TGCCAGTTTG TCTGCATTGC CCAGCAAGAT TACTGCCGTA TTTTGAACCA AGTAGAAAAG
    AACATGCAAA AAGTTGAGGA GGAAGGAGAG ATTGTAATGG TGAAGGAGCA CCGAGAACTT
    GATCGGACTG GAACAAGGAA GGGCCACATC GTCATCAAGG GCACCTCAGA GAGGCTGACC
    ATGCATTTGG TGGAAGAGCA TTCTGTAGTG GATCCAACAT TCATAGAAGA CTTCCTGTTG
    ACATACCGGA CTTTCCTTTC CAGCCCGATG GAAGTGGGCA AGAAGTTATT GGAGTGGTTC
    AATGACCCGA GCCTCAGGGA TAAGGTGACG CGGGTAGTAT TACTGTGGGT GAATAATCAC
    TTCAATGACT TTGAAGGGGA TCCTGCAATG ACTCGATTTC TGGAAGAATT TGAAAATAAT
    CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTGA ACATTGCATG TGCAGCTAAA
    GCCAAACGAA GACTGATGAC GTTAACAAAG CCTTCCCGGG AAGCCCCTCT GCCCTTCATC
    TTACTGGGAG GCTCTGAAAA GGGCTTTGGC ATCTTCGTGG ACAGCGTTGA CTCGTGTAGC
    AAAGCCACTG AAGCAGGCTT GAAGCGCGGG GATCAGATAT TAGAAGTGAA TGGTCAAAAT
    TTTGAAAATA TCCAACTTTC AAAGGCAATG GAAATTCTCA GAAATAACAC ACACCTGTCC
    ATCACTGTGA AAACCAACTT ATTTGTATTT AAAGAACTTC TGACGAGATT GTCTGAAGAG
    AAAAGAAACG GTGCCCCTCA CCTCCCTAAA ATCGGGGACA TCAAGAAGGC CAGCCGCTAC
    TCCATACCGG ATCTCGCCGT AGACGTAGAA CAGGTGATAG GCCTCGAGAA GGTCAACAAG
    AAAAGCAAAG CCAACACCGT GGGAGGAAGA AACAAGCTGA AGAAAATACT CGACAAGACC
    CGGATCAGCA TCTTACCTCA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GATAGCATCG TAGGACTGAG GCAGACCAAG CACATCCCAG CTGCCCTGCC TGTCAGTGGA
    ACGCTGTCCT CCAGTAACCC TGACTTGCTG CAGTCACACC ACCGGATTCT GGACTTCAGC
    ACTACTCCTG ACTTGCCAGA TCAGGTACTG AGAGTTTTCA AGGCTGACCA ACAAAGCAGG
    TACATCATGA TCAGCAAGGA CACCACCGCA AAGGAAGTGG TCATCCAGGC CATCAGAGAG
    TTTGCTGTCA CTGCCACCCC AGAGCAGTAT TCCCTCTGTG AGGTCTCAGT CACACCAGAG
    GGAGTTATCA AACAACGCCG ACTCCCAGAT CAGCTTTCCA AGCTTGCCGA CAGGATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACGCTTTG CTCAGATGAA
    GATGCCCAGG AGCTGCTGCG AGAGAGCCAA ATCTCCCTCC TTCAGCTCAG CACCGTGGAG
    GTTGCCACGC AGCTCTCCAT GAGGAACTTT GAACTCTTCC GCAACATTGA GCCTACTGAA
    TACATAGATG ATTTATTTAA ACTCAAATCA AAAACCAGCT GTGCCAACCT GAAGAAATTT
    GAGGAAGTCA TTAACCAGGA AACATTTTGG GTGGCTTCTG AAATCCTGCG GGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCCTTGCA CTGCAGGGAA
    TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCGGTGGCA
    AGACTACGAA CCACCTGGGA AAAGCTTCCC AATAAATATG AAAAGCTGTT CCAGGACCTG
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAGTACC GCAATGTCCT GAGTGGCCAG
    AACTTACAGC CTCCTGTCAT TCCCCTGTTC CCTGTCATCA AGAAGGACCT CACGTTTCTC
    CATGAGGGAA ATGACTCAAA AGTAGACGGA CTGGTCAACT TTGAGAAGCT GAGGATGATT
    GCAAAAGAAA TCCGTCACGT TGGCCGAATG GCATCAGTCA ACATGGACCC TGCCCTTATG
    TTCAGGACTC GGAAGAAGAA GTGGCGGAGT CTGGGGTCCC TGAGTCAGGG CAGTGCAAAC
    GCCACAGTGC TGGATGTTGC TCAGACAGGC GGGCATAAAA AGCGGGTACG CCGTAGTTCC
    TTCCTCAATG CCAAAAAGCT GTATGAAGAT GCCCAGATGG CTCGGAAAGT GAAGCAATAC
    CTGTCCAACC TGGAGCTGGA GATGGATGAG GAGAGTCTTC AGACACTGTC ACTGCAGTGT
    GAACCAGCAA CCAGCACATT GCCCAAGAAT CCTGGAGACA AAAAGCCTGT CAAGTCTGAG
    ACCTCCCCAG TGGCTCCACG TGCAGGGCCC CAGCAGAAAG TACAGCCTCA GCAGCCGCTG
    GCACAGCCAC AACCTCCACA TAAAGTCAGC CAGGGACTGC AGGTGCCCGC TGTGTCCCTC
    TATCCTTCAC GAAAGAAGGT GCCGGTGAAG GATCTGCCTC CTTTTGGCAT AAACTCTCCA
    CAAGCTTTGA AGAAAATCCT GTCCTTGTCT GAAGAAGGGA GTTTGGAGCG TCACAGGAAG
    CAAGCAGAAG ATACAATATC AAATGCGTCC TCTCAGCTCT CATCGCCCCC CACCTCACCT
    CAGAGCTCTC CAAGGAAAGG TTATGCACTG GCTCTCAGTG GCACCGTGGA TAATTTCTCA
    GATTCAGGTC ACAGTGAGAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCTTTTGAC
    TCGGTGCCAG TCTCTCTGCA TGATGAACGG CGCCAGAGGC ATTCTGTCAG CATTGTGGAG
    AGCAACCTAG GTGTGGGCAG GATGGAGCGG CGGACCCTGA TGGAGCCTGA TCAATACAGC
    TTAGGGTCGT ATGCCCCAGT GTCTGAAAGC CGAGGCCTGT ATGCCGCAGC GACGGTCATC
    TCTTCTCCTA GCACAGAGGA GCTTTCCCAT GACCAAGGGG ACCGTGCTTC CCTCGATGCT
    GCTGACAGCG GTCGTGGGAG CTGGACATCA TGCTCAAGTG GTTCCCATGA CAACATACAG
    ACCATTCAGC ACCAGAGAAG CTGGGAAACA CTGCCATTTG GGCACACTCA CTTTGACTAT
    TCAGGGGATG CTGCAAGTAT ATGGGCCTCC GGTGGCCATA TGGACCAAAT GATGTTTTCT
    GATCATAGCA CAAAATATAA CAGACAGAAT CAAAGTAGAG AGAGTCTTGA ACAAGCCCAA
    TCCCGGGCCA GCTGGGCGTC TTCCACGGGC TACTGGGGAG AAGACTCAGA AGGCGACACA
    GGCACTATCA AGCGAAGGGG TGGGAAGGAT GTCTCCGCTG AGGCAGAGAG CAGCAGCATG
    GTGCCCGTGA CTACAGAGGA AGCCAAACCT GTCCCTATGC CTGCCCACAT AGCTGTGACG
    CCGAGCACTA CCAAGGGACT CATCGCACGG AAGGAAGGCA GGTACCGGGA GCCGCCTCCC
    ACACCTCCAG GCTACGTGGG CATCCCCATT GCCGATTTCC CAGAAGGGCC TTGCCACCCG
    GCCAGGAAGC CCCCGGATTA CAACGTGGCC CTGCAGCGGT CCCGCATGGT GGCACGGCCC
    ACTGAGGCCC CGGCACCGGG CCAGACGCCG CCTGCAGCCG CAGCCAGCCG GCCGGGCAGC
    AAGCCACAGT GGCACAAGCC CAGCGACGCA GACCCACGCC TCGCGCCCTT CCAGCCGCAG
    GGCTTCGCAG GAGCGGAGGA GGACGAAGAT GAACAAGTGT CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu71468
    Clone ID Related Accession (Same CDS sequence) NM_001310536.1
    Accession Version NM_001310536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-15
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147368.1, BC067056.1 and BP758717.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream in-frame start site compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147368.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACCTGA GTGGACTGCC AGAGACGGCG GTTGACTCTG AGGATGACGA TGATGAGGAA 
    GACATCGAGA GAGCATCAGA CCCTCTGATG AGCAGGGACA TCGTGAGGGA CTGCCTGGAG
    AAGGACCCGA TAGACAGGAC TGACGATGAC ATCGAACAAC TACTGGAATT TATGCACCAG
    CTGCCGGCCT TTGCCAATAT GACGATGTCA GTAAGACGGG AGCTCTGTGC CGTGATGGTG
    TTTGCAGTGG TGGAAAGAGC TGGAACCATT GTGTTAAACG ACGGAGAAGA GCTGGATTCC
    TGGTCAGTGA TTCTCAATGG TTCCGTGGAG GTGACATATC CGGATGGGAA AGCAGAAATT
    CTGTGCATGG GAAACAGCTT TGGTGTTTCG CCCACCATGG ACAAAGAATA CATGAAAGGA
    GTCATGAGGA CTAAGGTGGA TGACTGCCAG TTTGTCTGCA TTGCCCAGCA AGATTACTGC
    CGTATTTTGA ACCAAGTAGA AAAGAACATG CAAAAAGTTG AGGAGGAAGG AGAGATTGTA
    ATGGTGAAGG AGCACCGAGA ACTTGATCGG ACTGGAACAA GGAAGGGCCA CATCGTCATC
    AAGGGCACCT CAGAGAGGCT GACCATGCAT TTGGTGGAAG AGCATTCTGT AGTGGATCCA
    ACATTCATAG AAGACTTCCT GTTGACATAC CGGACTTTCC TTTCCAGCCC GATGGAAGTG
    GGCAAGAAGT TATTGGAGTG GTTCAATGAC CCGAGCCTCA GGGATAAGGT GACGCGGGTA
    GTATTACTGT GGGTGAATAA TCACTTCAAT GACTTTGAAG GGGATCCTGC AATGACTCGA
    TTTCTGGAAG AATTTGAAAA TAATCTGGAA AGAGAGAAAA TGGGTGGACA TCTAAGGCTG
    TTGAACATTG CATGTGCAGC TAAAGCCAAA CGAAGACTGA TGACGTTAAC AAAGCCTTCC
    CGGGAAGCCC CTCTGCCCTT CATCTTACTG GGAGGCTCTG AAAAGGGCTT TGGCATCTTC
    GTGGACAGCG TTGACTCGTG TAGCAAAGCC ACTGAAGCAG GCTTGAAGCG CGGGGATCAG
    ATATTAGAAG TGAATGGTCA AAATTTTGAA AATATCCAAC TTTCAAAGGC AATGGAAATT
    CTCAGAAATA ACACACACCT GTCCATCACT GTGAAAACCA ACTTATTTGT ATTTAAAGAA
    CTTCTGACGA GATTGTCTGA AGAGAAAAGA AACGGTGCCC CTCACCTCCC TAAAATCGGG
    GACATCAAGA AGGCCAGCCG CTACTCCATA CCGGATCTCG CCGTAGACGT AGAACAGGTG
    ATAGGCCTCG AGAAGGTCAA CAAGAAAAGC AAAGCCAACA CCGTGGGAGG AAGAAACAAG
    CTGAAGAAAA TACTCGACAA GACCCGGATC AGCATCTTAC CTCAGAAACC ATACAATGAT
    ATTGGGATTG GTCAGTCTCA AGATGATAGC ATCGTAGGAC TGAGGCAGAC CAAGCACATC
    CCAGCTGCCC TGCCTGTCAG TGGAACGCTG TCCTCCAGTA ACCCTGACTT GCTGCAGTCA
    CACCACCGGA TTCTGGACTT CAGCACTACT CCTGACTTGC CAGATCAGGT ACTGAGAGTT
    TTCAAGGCTG ACCAACAAAG CAGGTACATC ATGATCAGCA AGGACACCAC CGCAAAGGAA
    GTGGTCATCC AGGCCATCAG AGAGTTTGCT GTCACTGCCA CCCCAGAGCA GTATTCCCTC
    TGTGAGGTCT CAGTCACACC AGAGGGAGTT ATCAAACAAC GCCGACTCCC AGATCAGCTT
    TCCAAGCTTG CCGACAGGAT ACAACTGAGT GGAAGGTATT ATCTGAAAAA CAACATGGAA
    ACAGAAACGC TTTGCTCAGA TGAAGATGCC CAGGAGCTGC TGCGAGAGAG CCAAATCTCC
    CTCCTTCAGC TCAGCACCGT GGAGGTTGCC ACGCAGCTCT CCATGAGGAA CTTTGAACTC
    TTCCGCAACA TTGAGCCTAC TGAATACATA GATGATTTAT TTAAACTCAA ATCAAAAACC
    AGCTGTGCCA ACCTGAAGAA ATTTGAGGAA GTCATTAACC AGGAAACATT TTGGGTGGCT
    TCTGAAATCC TGCGGGAAAC AAACCAGCTG AAGAGGATGA AGATCATTAA GCATTTCATC
    AAGATTGCCT TGCACTGCAG GGAATGCAAG AATTTTAACT CCATGTTTGC AATCATCAGT
    GGCCTAAACC TGGCACCGGT GGCAAGACTA CGAACCACCT GGGAAAAGCT TCCCAATAAA
    TATGAAAAGC TGTTCCAGGA CCTGCAAGAC CTGTTTGATC CTTCCAGAAA CATGGCAAAG
    TACCGCAATG TCCTGAGTGG CCAGAACTTA CAGCCTCCTG TCATTCCCCT GTTCCCTGTC
    ATCAAGAAGG ACCTCACGTT TCTCCATGAG GGAAATGACT CAAAAGTAGA CGGACTGGTC
    AACTTTGAGA AGCTGAGGAT GATTGCAAAA GAAATCCGTC ACGTTGGCCG AATGGCATCA
    GTCAACATGG ACCCTGCCCT TATGTTCAGG ACTCGGAAGA AGAAGTGGCG GAGTCTGGGG
    TCCCTGAGTC AGGGCAGTGC AAACGCCACA GTGCTGGATG TTGCTCAGAC AGGCGGGCAT
    AAAAAGCGGG TACGCCGTAG TTCCTTCCTC AATGCCAAAA AGCTGTATGA AGATGCCCAG
    ATGGCTCGGA AAGTGAAGCA ATACCTGTCC AACCTGGAGC TGGAGATGGA TGAGGAGAGT
    CTTCAGACAC TGTCACTGCA GTGTGAACCA GCAACCAGCA CATTGCCCAA GAATCCTGGA
    GACAAAAAGC CTGTCAAGTC TGAGACCTCC CCAGTGGCTC CACGTGCAGG GCCCCAGCAG
    AAAGTACAGC CTCAGCAGCC GCTGGCACAG CCACAACCTC CACATAAAGT CAGCCAGGGA
    CTGCAGGTGC CCGCTGTGTC CCTCTATCCT TCACGAAAGA AGGTGCCGGT GAAGGATCTG
    CCTCCTTTTG GCATAAACTC TCCACAAGCT TTGAAGAAAA TCCTGTCCTT GTCTGAAGAA
    GGGAGTTTGG AGCGTCACAG GAAGCAAGCA GAAGATACAA TATCAAATGC GTCCTCTCAG
    CTCTCATCGC CCCCCACCTC ACCTCAGAGC TCTCCAAGGA AAGGTTATGC ACTGGCTCTC
    AGTGGCACCG TGGATAATTT CTCAGATTCA GGTCACAGTG AGATTTCTTC ACGATCCAGT
    ATTGTCAGCA ATTCTTCTTT TGACTCGGTG CCAGTCTCTC TGCATGATGA ACGGCGCCAG
    AGGCATTCTG TCAGCATTGT GGAGAGCAAC CTAGGTGTGG GCAGGATGGA GCGGCGGACC
    CTGATGGAGC CTGATCAATA CAGCTTAGGG TCGTATGCCC CAGTGTCTGA AAGCCGAGGC
    CTGTATGCCG CAGCGACGGT CATCTCTTCT CCTAGCACAG AGGAGCTTTC CCATGACCAA
    GGGGACCGTG CTTCCCTCGA TGCTGCTGAC AGCGGTCGTG GGAGCTGGAC ATCATGCTCA
    AGTGGTTCCC ATGACAACAT ACAGACCATT CAGCACCAGA GAAGCTGGGA AACACTGCCA
    TTTGGGCACA CTCACTTTGA CTATTCAGGG GATGCTGCAA GTATATGGGC CTCCGGTGGC
    CATATGGACC AAATGATGTT TTCTGATCAT AGCACAAAAT ATAACAGACA GAATCAAAGT
    AGAGAGAGTC TTGAACAAGC CCAATCCCGG GCCAGCTGGG CGTCTTCCAC GGGCTACTGG
    GGAGAAGACT CAGAAGGCGA CACAGGCACT ATCAAGCGAA GGGGTGGGAA GGATGTCTCC
    GCTGAGGCAG AGAGCAGCAG CATGGTGCCC GTGACTACAG AGGAAGCCAA ACCTGTCCCT
    ATGCCTGCCC ACATAGCTGT GACGCCGAGC ACTACCAAGG GACTCATCGC ACGGAAGGAA
    GGCAGGTACC GGGAGCCGCC TCCCACACCT CCAGGCTACG TGGGCATCCC CATTGCCGAT
    TTCCCAGAAG GGCCTTGCCA CCCGGCCAGG AAGCCCCCGG ATTACAACGT GGCCCTGCAG
    CGGTCCCGCA TGGTGGCACG GCCCACTGAG GCCCCGGCAC CGGGCCAGAC GCCGCCTGCA
    GCCGCAGCCA GCCGGCCGGG CAGCAAGCCA CAGTGGCACA AGCCCAGCGA CGCAGACCCA
    CGCCTCGCGC CCTTCCAGCC GCAGGGCTTC GCAGGAGCGG AGGAGGACGA AGATGAACAA
    GTGTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001297465.1
    CDS189..4463
    Translation

    Target ORF information:

    RefSeq Version NM_001310536.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001310536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACCTGA GTGGACTGCC AGAGACGGCG GTTGACTCTG AGGATGACGA TGATGAGGAA 
    GACATCGAGA GAGCATCAGA CCCTCTGATG AGCAGGGACA TCGTGAGGGA CTGCCTGGAG
    AAGGACCCGA TAGACAGGAC TGACGATGAC ATCGAACAAC TACTGGAATT TATGCACCAG
    CTGCCGGCCT TTGCCAATAT GACGATGTCA GTAAGACGGG AGCTCTGTGC CGTGATGGTG
    TTTGCAGTGG TGGAAAGAGC TGGAACCATT GTGTTAAACG ACGGAGAAGA GCTGGATTCC
    TGGTCAGTGA TTCTCAATGG TTCCGTGGAG GTGACATATC CGGATGGGAA AGCAGAAATT
    CTGTGCATGG GAAACAGCTT TGGTGTTTCG CCCACCATGG ACAAAGAATA CATGAAAGGA
    GTCATGAGGA CTAAGGTGGA TGACTGCCAG TTTGTCTGCA TTGCCCAGCA AGATTACTGC
    CGTATTTTGA ACCAAGTAGA AAAGAACATG CAAAAAGTTG AGGAGGAAGG AGAGATTGTA
    ATGGTGAAGG AGCACCGAGA ACTTGATCGG ACTGGAACAA GGAAGGGCCA CATCGTCATC
    AAGGGCACCT CAGAGAGGCT GACCATGCAT TTGGTGGAAG AGCATTCTGT AGTGGATCCA
    ACATTCATAG AAGACTTCCT GTTGACATAC CGGACTTTCC TTTCCAGCCC GATGGAAGTG
    GGCAAGAAGT TATTGGAGTG GTTCAATGAC CCGAGCCTCA GGGATAAGGT GACGCGGGTA
    GTATTACTGT GGGTGAATAA TCACTTCAAT GACTTTGAAG GGGATCCTGC AATGACTCGA
    TTTCTGGAAG AATTTGAAAA TAATCTGGAA AGAGAGAAAA TGGGTGGACA TCTAAGGCTG
    TTGAACATTG CATGTGCAGC TAAAGCCAAA CGAAGACTGA TGACGTTAAC AAAGCCTTCC
    CGGGAAGCCC CTCTGCCCTT CATCTTACTG GGAGGCTCTG AAAAGGGCTT TGGCATCTTC
    GTGGACAGCG TTGACTCGTG TAGCAAAGCC ACTGAAGCAG GCTTGAAGCG CGGGGATCAG
    ATATTAGAAG TGAATGGTCA AAATTTTGAA AATATCCAAC TTTCAAAGGC AATGGAAATT
    CTCAGAAATA ACACACACCT GTCCATCACT GTGAAAACCA ACTTATTTGT ATTTAAAGAA
    CTTCTGACGA GATTGTCTGA AGAGAAAAGA AACGGTGCCC CTCACCTCCC TAAAATCGGG
    GACATCAAGA AGGCCAGCCG CTACTCCATA CCGGATCTCG CCGTAGACGT AGAACAGGTG
    ATAGGCCTCG AGAAGGTCAA CAAGAAAAGC AAAGCCAACA CCGTGGGAGG AAGAAACAAG
    CTGAAGAAAA TACTCGACAA GACCCGGATC AGCATCTTAC CTCAGAAACC ATACAATGAT
    ATTGGGATTG GTCAGTCTCA AGATGATAGC ATCGTAGGAC TGAGGCAGAC CAAGCACATC
    CCAGCTGCCC TGCCTGTCAG TGGAACGCTG TCCTCCAGTA ACCCTGACTT GCTGCAGTCA
    CACCACCGGA TTCTGGACTT CAGCACTACT CCTGACTTGC CAGATCAGGT ACTGAGAGTT
    TTCAAGGCTG ACCAACAAAG CAGGTACATC ATGATCAGCA AGGACACCAC CGCAAAGGAA
    GTGGTCATCC AGGCCATCAG AGAGTTTGCT GTCACTGCCA CCCCAGAGCA GTATTCCCTC
    TGTGAGGTCT CAGTCACACC AGAGGGAGTT ATCAAACAAC GCCGACTCCC AGATCAGCTT
    TCCAAGCTTG CCGACAGGAT ACAACTGAGT GGAAGGTATT ATCTGAAAAA CAACATGGAA
    ACAGAAACGC TTTGCTCAGA TGAAGATGCC CAGGAGCTGC TGCGAGAGAG CCAAATCTCC
    CTCCTTCAGC TCAGCACCGT GGAGGTTGCC ACGCAGCTCT CCATGAGGAA CTTTGAACTC
    TTCCGCAACA TTGAGCCTAC TGAATACATA GATGATTTAT TTAAACTCAA ATCAAAAACC
    AGCTGTGCCA ACCTGAAGAA ATTTGAGGAA GTCATTAACC AGGAAACATT TTGGGTGGCT
    TCTGAAATCC TGCGGGAAAC AAACCAGCTG AAGAGGATGA AGATCATTAA GCATTTCATC
    AAGATTGCCT TGCACTGCAG GGAATGCAAG AATTTTAACT CCATGTTTGC AATCATCAGT
    GGCCTAAACC TGGCACCGGT GGCAAGACTA CGAACCACCT GGGAAAAGCT TCCCAATAAA
    TATGAAAAGC TGTTCCAGGA CCTGCAAGAC CTGTTTGATC CTTCCAGAAA CATGGCAAAG
    TACCGCAATG TCCTGAGTGG CCAGAACTTA CAGCCTCCTG TCATTCCCCT GTTCCCTGTC
    ATCAAGAAGG ACCTCACGTT TCTCCATGAG GGAAATGACT CAAAAGTAGA CGGACTGGTC
    AACTTTGAGA AGCTGAGGAT GATTGCAAAA GAAATCCGTC ACGTTGGCCG AATGGCATCA
    GTCAACATGG ACCCTGCCCT TATGTTCAGG ACTCGGAAGA AGAAGTGGCG GAGTCTGGGG
    TCCCTGAGTC AGGGCAGTGC AAACGCCACA GTGCTGGATG TTGCTCAGAC AGGCGGGCAT
    AAAAAGCGGG TACGCCGTAG TTCCTTCCTC AATGCCAAAA AGCTGTATGA AGATGCCCAG
    ATGGCTCGGA AAGTGAAGCA ATACCTGTCC AACCTGGAGC TGGAGATGGA TGAGGAGAGT
    CTTCAGACAC TGTCACTGCA GTGTGAACCA GCAACCAGCA CATTGCCCAA GAATCCTGGA
    GACAAAAAGC CTGTCAAGTC TGAGACCTCC CCAGTGGCTC CACGTGCAGG GCCCCAGCAG
    AAAGTACAGC CTCAGCAGCC GCTGGCACAG CCACAACCTC CACATAAAGT CAGCCAGGGA
    CTGCAGGTGC CCGCTGTGTC CCTCTATCCT TCACGAAAGA AGGTGCCGGT GAAGGATCTG
    CCTCCTTTTG GCATAAACTC TCCACAAGCT TTGAAGAAAA TCCTGTCCTT GTCTGAAGAA
    GGGAGTTTGG AGCGTCACAG GAAGCAAGCA GAAGATACAA TATCAAATGC GTCCTCTCAG
    CTCTCATCGC CCCCCACCTC ACCTCAGAGC TCTCCAAGGA AAGGTTATGC ACTGGCTCTC
    AGTGGCACCG TGGATAATTT CTCAGATTCA GGTCACAGTG AGATTTCTTC ACGATCCAGT
    ATTGTCAGCA ATTCTTCTTT TGACTCGGTG CCAGTCTCTC TGCATGATGA ACGGCGCCAG
    AGGCATTCTG TCAGCATTGT GGAGAGCAAC CTAGGTGTGG GCAGGATGGA GCGGCGGACC
    CTGATGGAGC CTGATCAATA CAGCTTAGGG TCGTATGCCC CAGTGTCTGA AAGCCGAGGC
    CTGTATGCCG CAGCGACGGT CATCTCTTCT CCTAGCACAG AGGAGCTTTC CCATGACCAA
    GGGGACCGTG CTTCCCTCGA TGCTGCTGAC AGCGGTCGTG GGAGCTGGAC ATCATGCTCA
    AGTGGTTCCC ATGACAACAT ACAGACCATT CAGCACCAGA GAAGCTGGGA AACACTGCCA
    TTTGGGCACA CTCACTTTGA CTATTCAGGG GATGCTGCAA GTATATGGGC CTCCGGTGGC
    CATATGGACC AAATGATGTT TTCTGATCAT AGCACAAAAT ATAACAGACA GAATCAAAGT
    AGAGAGAGTC TTGAACAAGC CCAATCCCGG GCCAGCTGGG CGTCTTCCAC GGGCTACTGG
    GGAGAAGACT CAGAAGGCGA CACAGGCACT ATCAAGCGAA GGGGTGGGAA GGATGTCTCC
    GCTGAGGCAG AGAGCAGCAG CATGGTGCCC GTGACTACAG AGGAAGCCAA ACCTGTCCCT
    ATGCCTGCCC ACATAGCTGT GACGCCGAGC ACTACCAAGG GACTCATCGC ACGGAAGGAA
    GGCAGGTACC GGGAGCCGCC TCCCACACCT CCAGGCTACG TGGGCATCCC CATTGCCGAT
    TTCCCAGAAG GGCCTTGCCA CCCGGCCAGG AAGCCCCCGG ATTACAACGT GGCCCTGCAG
    CGGTCCCGCA TGGTGGCACG GCCCACTGAG GCCCCGGCAC CGGGCCAGAC GCCGCCTGCA
    GCCGCAGCCA GCCGGCCGGG CAGCAAGCCA CAGTGGCACA AGCCCAGCGA CGCAGACCCA
    CGCCTCGCGC CCTTCCAGCC GCAGGGCTTC GCAGGAGCGG AGGAGGACGA AGATGAACAA
    GTGTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32397
    Clone ID Related Accession (Same CDS sequence) XM_006502250.3 , XM_006502250.4
    Accession Version XM_006502250.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGCTCCC TGCAGACTTC
    ACAAAACTGC ACCTCACTGA CAGCCTGCAC CCACAGGTGA CCCACGTGTC CTCCAGCCAT
    TCAGGATGTA GCATCACCAG CGACTCTGGG AGCAGCAGTC TTTCTGACAT TTACCAGGCC
    ACGGAGAGCG AGGCCGGTGA CATGGACCTG AGTGGACTGC CAGAGACGGC GGTTGACTCT
    GAGGATGACG ATGATGAGGA AGACATCGAG AGAGCATCAG ACCCTCTGAT GAGCAGGGAC
    ATCGTGAGGG ACTGCCTGGA GAAGGACCCG ATAGACAGGA CTGACGATGA CATCGAACAA
    CTACTGGAAT TTATGCACCA GCTGCCGGCC TTTGCCAATA TGACGATGTC AGTAAGACGG
    GAGCTCTGTG CCGTGATGGT GTTTGCAGTG GTGGAAAGAG CTGGAACCAT TGTGTTAAAC
    GACGGAGAAG AGCTGGATTC CTGGTCAGTG ATTCTCAATG GTTCCGTGGA GGTGACATAT
    CCGGATGGGA AAGCAGAAAT TCTGTGCATG GGAAACAGCT TTGGTGTTTC GCCCACCATG
    GACAAAGAAT ACATGAAAGG AGTCATGAGG ACTAAGGTGG ATGACTGCCA GTTTGTCTGC
    ATTGCCCAGC AAGATTACTG CCGTATTTTG AACCAAGTAG AAAAGAACAT GCAAAAAGTT
    GAGGAGGAAG GAGAGATTGT AATGGTGAAG GAGCACCGAG AACTTGATCG GACTGGAACA
    AGGAAGGGCC ACATCGTCAT CAAGGGCACC TCAGAGAGGC TGACCATGCA TTTGGTGGAA
    GAGCATTCTG TAGTGGATCC AACATTCATA GAAGACTTCC TGTTGACATA CCGGACTTTC
    CTTTCCAGCC CGATGGAAGT GGGCAAGAAG TTATTGGAGT GGTTCAATGA CCCGAGCCTC
    AGGGATAAGG TGACGCGGGT AGTATTACTG TGGGTGAATA ATCACTTCAA TGACTTTGAA
    GGGGATCCTG CAATGACTCG ATTTCTGGAA GAATTTGAAA ATAATCTGGA AAGAGAGAAA
    ATGGGTGGAC ATCTAAGGCT GTTGAACATT GCATGTGCAG CTAAAGCCAA ACGAAGACTG
    ATGACGTTAA CAAAGCCTTC CCGGGAAGCC CCTCTGCCCT TCATCTTACT GGGAGGCTCT
    GAAAAGGGCT TTGGCATCTT CGTGGACAGC GTTGACTCGT GTAGCAAAGC CACTGAAGCA
    GGCTTGAAGC GCGGGGATCA GATATTAGAA GTGAATGGTC AAAATTTTGA AAATATCCAA
    CTTTCAAAGG CAATGGAAAT TCTCAGAAAT AACACACACC TGTCCATCAC TGTGAAAACC
    AACTTATTTG TATTTAAAGA ACTTCTGACG AGATTGTCTG AAGAGAAAAG AAACGGTGCC
    CCTCACCTCC CTAAAATCGG GGACATCAAG AAGGCCAGCC GCTACTCCAT ACCGGATCTC
    GCCGTAGACG TAGAACAGGT GATAGGCCTC GAGAAGGTCA ACAAGAAAAG CAAAGCCAAC
    ACCGTGGGAG GAAGAAACAA GCTGAAGAAA ATACTCGACA AGACCCGGAT CAGCATCTTA
    CCTCAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGATAG CATCGTAGGA
    CTGAGGCAGA CCAAGCACAT CCCAGCTGCC CTGCCTGTCA GTGGAACGCT GTCCTCCAGT
    AACCCTGACT TGCTGCAGTC ACACCACCGG ATTCTGGACT TCAGCACTAC TCCTGACTTG
    CCAGATCAGG TACTGAGAGT TTTCAAGGCT GACCAACAAA GCAGGTACAT CATGATCAGC
    AAGGACACCA CCGCAAAGGA AGTGGTCATC CAGGCCATCA GAGAGTTTGC TGTCACTGCC
    ACCCCAGAGC AGTATTCCCT CTGTGAGGTC TCAGTCACAC CAGAGGGAGT TATCAAACAA
    CGCCGACTCC CAGATCAGCT TTCCAAGCTT GCCGACAGGA TACAACTGAG TGGAAGGTAT
    TATCTGAAAA ACAACATGGA AACAGAAACG CTTTGCTCAG ATGAAGATGC CCAGGAGCTG
    CTGCGAGAGA GCCAAATCTC CCTCCTTCAG CTCAGCACCG TGGAGGTTGC CACGCAGCTC
    TCCATGAGGA ACTTTGAACT CTTCCGCAAC ATTGAGCCTA CTGAATACAT AGATGATTTA
    TTTAAACTCA AATCAAAAAC CAGCTGTGCC AACCTGAAGA AATTTGAGGA AGTCATTAAC
    CAGGAAACAT TTTGGGTGGC TTCTGAAATC CTGCGGGAAA CAAACCAGCT GAAGAGGATG
    AAGATCATTA AGCATTTCAT CAAGATTGCC TTGCACTGCA GGGAATGCAA GAATTTTAAC
    TCCATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCGG TGGCAAGACT ACGAACCACC
    TGGGAAAAGC TTCCCAATAA ATATGAAAAG CTGTTCCAGG ACCTGCAAGA CCTGTTTGAT
    CCTTCCAGAA ACATGGCAAA GTACCGCAAT GTCCTGAGTG GCCAGAACTT ACAGCCTCCT
    GTCATTCCCC TGTTCCCTGT CATCAAGAAG GACCTCACGT TTCTCCATGA GGGAAATGAC
    TCAAAAGTAG ACGGACTGGT CAACTTTGAG AAGCTGAGGA TGATTGCAAA AGAAATCCGT
    CACGTTGGCC GAATGGCATC AGTCAACATG GACCCTGCCC TTATGTTCAG GACTCGGAAG
    AAGAAGTGGC GGAGTCTGGG GTCCCTGAGT CAGGGCAGTG CAAACGCCAC AGTGCTGGAT
    GTTGCTCAGA CAGGCGGGCA TAAAAAGCGG GTACGCCGTA GTTCCTTCCT CAATGCCAAA
    AAGCTGTATG AAGATGCCCA GATGGCTCGG AAAGTGAAGC AATACCTGTC CAACCTGGAG
    CTGGAGATGG ATGAGGAGAG TCTTCAGACA CTGTCACTGC AGTGTGAACC AGCAACCAGC
    ACATTGCCCA AGAATCCTGG AGACAAAAAG CCTGTCAAGT CTGAGACCTC CCCAGTGGCT
    CCACGTGCAG GGCCCCAGCA GAAAGTACAG CCTCAGCAGC CGCTGGCACA GCCACAACCT
    CCACATAAAG TCAGCCAGGG ACTGCAGGTG CCCGCTGTGT CCCTCTATCC TTCACGAAAG
    AAGGTGCCGG TGAAGGATCT GCCTCCTTTT GGCATAAACT CTCCACAAGC TTTGAAGAAA
    ATCCTGTCCT TGTCTGAAGA AGGGAGTTTG GAGCGTCACA GGAAGCAAGC AGAAGATACA
    ATATCAAATG CGTCCTCTCA GCTCTCATCG CCCCCCACCT CACCTCAGAG CTCTCCAAGG
    AAAGGTTATG CACTGGCTCT CAGTGGCACC GTGGATAATT TCTCAGATTC AGGTCACAGT
    GAGATTTCTT CACGATCCAG TATTGTCAGC AATTCTTCTT TTGACTCGGT GCCAGTCTCT
    CTGCATGATG AACGGCGCCA GAGGCATTCT GTCAGCATTG TGGAGAGCAA CCTAGGTGTG
    GGCAGGATGG AGCGGCGGAC CCTGATGGAG CCTGATCAAT ACAGCTTAGG GTCGTATGCC
    CCAGTGTCTG AAAGCCGAGG CCTGTATGCC GCAGCGACGG TCATCTCTTC TCCTAGCACA
    GAGGAGCTTT CCCATGACCA AGGGGACCGT GCTTCCCTCG ATGCTGCTGA CAGCGGTCGT
    GGGAGCTGGA CATCATGCTC AAGTGGTTCC CATGACAACA TACAGACCAT TCAGCACCAG
    AGAAGCTGGG AAACACTGCC ATTTGGGCAC ACTCACTTTG ACTATTCAGG GGATGCTGCA
    AGTATATGGG CCTCCGGTGG CCATATGGAC CAAATGATGT TTTCTGATCA TAGCACAAAA
    TATAACAGAC AGAATCAAAG TAGAGAGAGT CTTGAACAAG CCCAATCCCG GGCCAGCTGG
    GCGTCTTCCA CGGGCTACTG GGGAGAAGAC TCAGAAGGCG ACACAGGCAC TATCAAGCGA
    AGGGGTGGGA AGGATGTCTC CGCTGAGGCA GAGAGCAGCA GCATGGTGCC CGTGACTACA
    GAGGAAGCCA AACCTGTCCC TATGCCTGCC CACATAGCTG TGACGCCGAG CACTACCAAG
    GGACTCATCG CACGGAAGGA AGGCAGGTAC CGGGAGCCGC CTCCCACACC TCCAGGCTAC
    GTGGGCATCC CCATTGCCGA TTTCCCAGAA GGGCCTTGCC ACCCGGCCAG GAAGCCCCCG
    GATTACAACG TGGCCCTGCA GCGGTCCCGC ATGGTGGCAC GGCCCACTGA GGCCCCGGCA
    CCGGGCCAGA CGCCGCCTGC AGCCGCAGCC AGCCGGCCGG GCAGCAAGCC ACAGTGGCAC
    AAGCCCAGCG ACGCAGACCC ACGCCTCGCG CCCTTCCAGC CGCAGGGCTT CGCAGGAGCG
    GAGGAGGACG AAGATGAACA AGTGTCTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006502313.1
    CDS710..5665
    Translation

    Target ORF information:

    RefSeq Version XM_006502250.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006502250.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGCTCCC TGCAGACTTC
    ACAAAACTGC ACCTCACTGA CAGCCTGCAC CCACAGGTGA CCCACGTGTC CTCCAGCCAT
    TCAGGATGTA GCATCACCAG CGACTCTGGG AGCAGCAGTC TTTCTGACAT TTACCAGGCC
    ACGGAGAGCG AGGCCGGTGA CATGGACCTG AGTGGACTGC CAGAGACGGC GGTTGACTCT
    GAGGATGACG ATGATGAGGA AGACATCGAG AGAGCATCAG ACCCTCTGAT GAGCAGGGAC
    ATCGTGAGGG ACTGCCTGGA GAAGGACCCG ATAGACAGGA CTGACGATGA CATCGAACAA
    CTACTGGAAT TTATGCACCA GCTGCCGGCC TTTGCCAATA TGACGATGTC AGTAAGACGG
    GAGCTCTGTG CCGTGATGGT GTTTGCAGTG GTGGAAAGAG CTGGAACCAT TGTGTTAAAC
    GACGGAGAAG AGCTGGATTC CTGGTCAGTG ATTCTCAATG GTTCCGTGGA GGTGACATAT
    CCGGATGGGA AAGCAGAAAT TCTGTGCATG GGAAACAGCT TTGGTGTTTC GCCCACCATG
    GACAAAGAAT ACATGAAAGG AGTCATGAGG ACTAAGGTGG ATGACTGCCA GTTTGTCTGC
    ATTGCCCAGC AAGATTACTG CCGTATTTTG AACCAAGTAG AAAAGAACAT GCAAAAAGTT
    GAGGAGGAAG GAGAGATTGT AATGGTGAAG GAGCACCGAG AACTTGATCG GACTGGAACA
    AGGAAGGGCC ACATCGTCAT CAAGGGCACC TCAGAGAGGC TGACCATGCA TTTGGTGGAA
    GAGCATTCTG TAGTGGATCC AACATTCATA GAAGACTTCC TGTTGACATA CCGGACTTTC
    CTTTCCAGCC CGATGGAAGT GGGCAAGAAG TTATTGGAGT GGTTCAATGA CCCGAGCCTC
    AGGGATAAGG TGACGCGGGT AGTATTACTG TGGGTGAATA ATCACTTCAA TGACTTTGAA
    GGGGATCCTG CAATGACTCG ATTTCTGGAA GAATTTGAAA ATAATCTGGA AAGAGAGAAA
    ATGGGTGGAC ATCTAAGGCT GTTGAACATT GCATGTGCAG CTAAAGCCAA ACGAAGACTG
    ATGACGTTAA CAAAGCCTTC CCGGGAAGCC CCTCTGCCCT TCATCTTACT GGGAGGCTCT
    GAAAAGGGCT TTGGCATCTT CGTGGACAGC GTTGACTCGT GTAGCAAAGC CACTGAAGCA
    GGCTTGAAGC GCGGGGATCA GATATTAGAA GTGAATGGTC AAAATTTTGA AAATATCCAA
    CTTTCAAAGG CAATGGAAAT TCTCAGAAAT AACACACACC TGTCCATCAC TGTGAAAACC
    AACTTATTTG TATTTAAAGA ACTTCTGACG AGATTGTCTG AAGAGAAAAG AAACGGTGCC
    CCTCACCTCC CTAAAATCGG GGACATCAAG AAGGCCAGCC GCTACTCCAT ACCGGATCTC
    GCCGTAGACG TAGAACAGGT GATAGGCCTC GAGAAGGTCA ACAAGAAAAG CAAAGCCAAC
    ACCGTGGGAG GAAGAAACAA GCTGAAGAAA ATACTCGACA AGACCCGGAT CAGCATCTTA
    CCTCAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGATAG CATCGTAGGA
    CTGAGGCAGA CCAAGCACAT CCCAGCTGCC CTGCCTGTCA GTGGAACGCT GTCCTCCAGT
    AACCCTGACT TGCTGCAGTC ACACCACCGG ATTCTGGACT TCAGCACTAC TCCTGACTTG
    CCAGATCAGG TACTGAGAGT TTTCAAGGCT GACCAACAAA GCAGGTACAT CATGATCAGC
    AAGGACACCA CCGCAAAGGA AGTGGTCATC CAGGCCATCA GAGAGTTTGC TGTCACTGCC
    ACCCCAGAGC AGTATTCCCT CTGTGAGGTC TCAGTCACAC CAGAGGGAGT TATCAAACAA
    CGCCGACTCC CAGATCAGCT TTCCAAGCTT GCCGACAGGA TACAACTGAG TGGAAGGTAT
    TATCTGAAAA ACAACATGGA AACAGAAACG CTTTGCTCAG ATGAAGATGC CCAGGAGCTG
    CTGCGAGAGA GCCAAATCTC CCTCCTTCAG CTCAGCACCG TGGAGGTTGC CACGCAGCTC
    TCCATGAGGA ACTTTGAACT CTTCCGCAAC ATTGAGCCTA CTGAATACAT AGATGATTTA
    TTTAAACTCA AATCAAAAAC CAGCTGTGCC AACCTGAAGA AATTTGAGGA AGTCATTAAC
    CAGGAAACAT TTTGGGTGGC TTCTGAAATC CTGCGGGAAA CAAACCAGCT GAAGAGGATG
    AAGATCATTA AGCATTTCAT CAAGATTGCC TTGCACTGCA GGGAATGCAA GAATTTTAAC
    TCCATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCGG TGGCAAGACT ACGAACCACC
    TGGGAAAAGC TTCCCAATAA ATATGAAAAG CTGTTCCAGG ACCTGCAAGA CCTGTTTGAT
    CCTTCCAGAA ACATGGCAAA GTACCGCAAT GTCCTGAGTG GCCAGAACTT ACAGCCTCCT
    GTCATTCCCC TGTTCCCTGT CATCAAGAAG GACCTCACGT TTCTCCATGA GGGAAATGAC
    TCAAAAGTAG ACGGACTGGT CAACTTTGAG AAGCTGAGGA TGATTGCAAA AGAAATCCGT
    CACGTTGGCC GAATGGCATC AGTCAACATG GACCCTGCCC TTATGTTCAG GACTCGGAAG
    AAGAAGTGGC GGAGTCTGGG GTCCCTGAGT CAGGGCAGTG CAAACGCCAC AGTGCTGGAT
    GTTGCTCAGA CAGGCGGGCA TAAAAAGCGG GTACGCCGTA GTTCCTTCCT CAATGCCAAA
    AAGCTGTATG AAGATGCCCA GATGGCTCGG AAAGTGAAGC AATACCTGTC CAACCTGGAG
    CTGGAGATGG ATGAGGAGAG TCTTCAGACA CTGTCACTGC AGTGTGAACC AGCAACCAGC
    ACATTGCCCA AGAATCCTGG AGACAAAAAG CCTGTCAAGT CTGAGACCTC CCCAGTGGCT
    CCACGTGCAG GGCCCCAGCA GAAAGTACAG CCTCAGCAGC CGCTGGCACA GCCACAACCT
    CCACATAAAG TCAGCCAGGG ACTGCAGGTG CCCGCTGTGT CCCTCTATCC TTCACGAAAG
    AAGGTGCCGG TGAAGGATCT GCCTCCTTTT GGCATAAACT CTCCACAAGC TTTGAAGAAA
    ATCCTGTCCT TGTCTGAAGA AGGGAGTTTG GAGCGTCACA GGAAGCAAGC AGAAGATACA
    ATATCAAATG CGTCCTCTCA GCTCTCATCG CCCCCCACCT CACCTCAGAG CTCTCCAAGG
    AAAGGTTATG CACTGGCTCT CAGTGGCACC GTGGATAATT TCTCAGATTC AGGTCACAGT
    GAGATTTCTT CACGATCCAG TATTGTCAGC AATTCTTCTT TTGACTCGGT GCCAGTCTCT
    CTGCATGATG AACGGCGCCA GAGGCATTCT GTCAGCATTG TGGAGAGCAA CCTAGGTGTG
    GGCAGGATGG AGCGGCGGAC CCTGATGGAG CCTGATCAAT ACAGCTTAGG GTCGTATGCC
    CCAGTGTCTG AAAGCCGAGG CCTGTATGCC GCAGCGACGG TCATCTCTTC TCCTAGCACA
    GAGGAGCTTT CCCATGACCA AGGGGACCGT GCTTCCCTCG ATGCTGCTGA CAGCGGTCGT
    GGGAGCTGGA CATCATGCTC AAGTGGTTCC CATGACAACA TACAGACCAT TCAGCACCAG
    AGAAGCTGGG AAACACTGCC ATTTGGGCAC ACTCACTTTG ACTATTCAGG GGATGCTGCA
    AGTATATGGG CCTCCGGTGG CCATATGGAC CAAATGATGT TTTCTGATCA TAGCACAAAA
    TATAACAGAC AGAATCAAAG TAGAGAGAGT CTTGAACAAG CCCAATCCCG GGCCAGCTGG
    GCGTCTTCCA CGGGCTACTG GGGAGAAGAC TCAGAAGGCG ACACAGGCAC TATCAAGCGA
    AGGGGTGGGA AGGATGTCTC CGCTGAGGCA GAGAGCAGCA GCATGGTGCC CGTGACTACA
    GAGGAAGCCA AACCTGTCCC TATGCCTGCC CACATAGCTG TGACGCCGAG CACTACCAAG
    GGACTCATCG CACGGAAGGA AGGCAGGTAC CGGGAGCCGC CTCCCACACC TCCAGGCTAC
    GTGGGCATCC CCATTGCCGA TTTCCCAGAA GGGCCTTGCC ACCCGGCCAG GAAGCCCCCG
    GATTACAACG TGGCCCTGCA GCGGTCCCGC ATGGTGGCAC GGCCCACTGA GGCCCCGGCA
    CCGGGCCAGA CGCCGCCTGC AGCCGCAGCC AGCCGGCCGG GCAGCAAGCC ACAGTGGCAC
    AAGCCCAGCG ACGCAGACCC ACGCCTCGCG CCCTTCCAGC CGCAGGGCTT CGCAGGAGCG
    GAGGAGGACG AAGATGAACA AGTGTCTGCT GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32398
    Clone ID Related Accession (Same CDS sequence) XM_006502252.3 , XM_006502252.4
    Accession Version XM_006502252.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TCCAACGTCA AGCCTCCCAC 
    AGACAGTCAC GGAGAAGATT TAGAAAGATC AACCAGAAAG GTGAAAGACA AACAATTATC
    GACACAGTGG ACCCTTACCC TGTGGGCAAG CCACCTTTAC CTAGAGGCTA CCATACGGAA
    TGCACTAAAT CTCAGCTCCC TGCAGACTTC ACAAAACTGC ACCTCACTGA CAGCCTGCAC
    CCACAGGTGA CCCACGTGTC CTCCAGCCAT TCAGGATGTA GCATCACCAG CGACTCTGGG
    AGCAGCAGTC TTTCTGACAT TTACCAGGCC ACGGAGAGCG AGGCCGGTGA CATGGACCTG
    AGTGGACTGC CAGAGACGGC GGTTGACTCT GAGGATGACG ATGATGAGGA AGACATCGAG
    AGAGCATCAG ACCCTCTGAT GAGCAGGGAC ATCGTGAGGG ACTGCCTGGA GAAGGACCCG
    ATAGACAGGA CTGACGATGA CATCGAACAA CTACTGGAAT TTATGCACCA GCTGCCGGCC
    TTTGCCAATA TGACGATGTC AGTAAGACGG GAGCTCTGTG CCGTGATGGT GTTTGCAGTG
    GTGGAAAGAG CTGGAACCAT TGTGTTAAAC GACGGAGAAG AGCTGGATTC CTGGTCAGTG
    ATTCTCAATG GTTCCGTGGA GGTGACATAT CCGGATGGGA AAGCAGAAAT TCTGTGCATG
    GGAAACAGCT TTGGTGTTTC GCCCACCATG GACAAAGAAT ACATGAAAGG AGTCATGAGG
    ACTAAGGTGG ATGACTGCCA GTTTGTCTGC ATTGCCCAGC AAGATTACTG CCGTATTTTG
    AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAGGAGGAAG GAGAGATTGT AATGGTGAAG
    GAGCACCGAG AACTTGATCG GACTGGAACA AGGAAGGGCC ACATCGTCAT CAAGGGCACC
    TCAGAGAGGC TGACCATGCA TTTGGTGGAA GAGCATTCTG TAGTGGATCC AACATTCATA
    GAAGACTTCC TGTTGACATA CCGGACTTTC CTTTCCAGCC CGATGGAAGT GGGCAAGAAG
    TTATTGGAGT GGTTCAATGA CCCGAGCCTC AGGGATAAGG TGACGCGGGT AGTATTACTG
    TGGGTGAATA ATCACTTCAA TGACTTTGAA GGGGATCCTG CAATGACTCG ATTTCTGGAA
    GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTGAACATT
    GCATGTGCAG CTAAAGCCAA ACGAAGACTG ATGACGTTAA CAAAGCCTTC CCGGGAAGCC
    CCTCTGCCCT TCATCTTACT GGGAGGCTCT GAAAAGGGCT TTGGCATCTT CGTGGACAGC
    GTTGACTCGT GTAGCAAAGC CACTGAAGCA GGCTTGAAGC GCGGGGATCA GATATTAGAA
    GTGAATGGTC AAAATTTTGA AAATATCCAA CTTTCAAAGG CAATGGAAAT TCTCAGAAAT
    AACACACACC TGTCCATCAC TGTGAAAACC AACTTATTTG TATTTAAAGA ACTTCTGACG
    AGATTGTCTG AAGAGAAAAG AAACGGTGCC CCTCACCTCC CTAAAATCGG GGACATCAAG
    AAGGCCAGCC GCTACTCCAT ACCGGATCTC GCCGTAGACG TAGAACAGGT GATAGGCCTC
    GAGAAGGTCA ACAAGAAAAG CAAAGCCAAC ACCGTGGGAG GAAGAAACAA GCTGAAGAAA
    ATACTCGACA AGACCCGGAT CAGCATCTTA CCTCAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGATAG CATCGTAGGA CTGAGGCAGA CCAAGCACAT CCCAGCTGCC
    CTGCCTGTCA GTGGAACGCT GTCCTCCAGT AACCCTGACT TGCTGCAGTC ACACCACCGG
    ATTCTGGACT TCAGCACTAC TCCTGACTTG CCAGATCAGG TACTGAGAGT TTTCAAGGCT
    GACCAACAAA GCAGGTACAT CATGATCAGC AAGGACACCA CCGCAAAGGA AGTGGTCATC
    CAGGCCATCA GAGAGTTTGC TGTCACTGCC ACCCCAGAGC AGTATTCCCT CTGTGAGGTC
    TCAGTCACAC CAGAGGGAGT TATCAAACAA CGCCGACTCC CAGATCAGCT TTCCAAGCTT
    GCCGACAGGA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACG
    CTTTGCTCAG ATGAAGATGC CCAGGAGCTG CTGCGAGAGA GCCAAATCTC CCTCCTTCAG
    CTCAGCACCG TGGAGGTTGC CACGCAGCTC TCCATGAGGA ACTTTGAACT CTTCCGCAAC
    ATTGAGCCTA CTGAATACAT AGATGATTTA TTTAAACTCA AATCAAAAAC CAGCTGTGCC
    AACCTGAAGA AATTTGAGGA AGTCATTAAC CAGGAAACAT TTTGGGTGGC TTCTGAAATC
    CTGCGGGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATTGCC
    TTGCACTGCA GGGAATGCAA GAATTTTAAC TCCATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCGG TGGCAAGACT ACGAACCACC TGGGAAAAGC TTCCCAATAA ATATGAAAAG
    CTGTTCCAGG ACCTGCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA GTACCGCAAT
    GTCCTGAGTG GCCAGAACTT ACAGCCTCCT GTCATTCCCC TGTTCCCTGT CATCAAGAAG
    GACCTCACGT TTCTCCATGA GGGAAATGAC TCAAAAGTAG ACGGACTGGT CAACTTTGAG
    AAGCTGAGGA TGATTGCAAA AGAAATCCGT CACGTTGGCC GAATGGCATC AGTCAACATG
    GACCCTGCCC TTATGTTCAG GACTCGGAAG AAGAAGTGGC GGAGTCTGGG GTCCCTGAGT
    CAGGGCAGTG CAAACGCCAC AGTGCTGGAT GTTGCTCAGA CAGGCGGGCA TAAAAAGCGG
    GTACGCCGTA GTTCCTTCCT CAATGCCAAA AAGCTGTATG AAGATGCCCA GATGGCTCGG
    AAAGTGAAGC AATACCTGTC CAACCTGGAG CTGGAGATGG ATGAGGAGAG TCTTCAGACA
    CTGTCACTGC AGTGTGAACC AGCAACCAGC ACATTGCCCA AGAATCCTGG AGACAAAAAG
    CCTGTCAAGT CTGAGACCTC CCCAGTGGCT CCACGTGCAG GGCCCCAGCA GAAAGTACAG
    CCTCAGCAGC CGCTGGCACA GCCACAACCT CCACATAAAG TCAGCCAGGG ACTGCAGGTG
    CCCGCTGTGT CCCTCTATCC TTCACGAAAG AAGGTGCCGG TGAAGGATCT GCCTCCTTTT
    GGCATAAACT CTCCACAAGC TTTGAAGAAA ATCCTGTCCT TGTCTGAAGA AGGGAGTTTG
    GAGCGTCACA GGAAGCAAGC AGAAGATACA ATATCAAATG CGTCCTCTCA GCTCTCATCG
    CCCCCCACCT CACCTCAGAG CTCTCCAAGG AAAGGTTATG CACTGGCTCT CAGTGGCACC
    GTGGATAATT TCTCAGATTC AGGTCACAGT GAGATTTCTT CACGATCCAG TATTGTCAGC
    AATTCTTCTT TTGACTCGGT GCCAGTCTCT CTGCATGATG AACGGCGCCA GAGGCATTCT
    GTCAGCATTG TGGAGAGCAA CCTAGGTGTG GGCAGGATGG AGCGGCGGAC CCTGATGGAG
    CCTGATCAAT ACAGCTTAGG GTCGTATGCC CCAGTGTCTG AAAGCCGAGG CCTGTATGCC
    GCAGCGACGG TCATCTCTTC TCCTAGCACA GAGGAGCTTT CCCATGACCA AGGGGACCGT
    GCTTCCCTCG ATGCTGCTGA CAGCGGTCGT GGGAGCTGGA CATCATGCTC AAGTGGTTCC
    CATGACAACA TACAGACCAT TCAGCACCAG AGAAGCTGGG AAACACTGCC ATTTGGGCAC
    ACTCACTTTG ACTATTCAGG GGATGCTGCA AGTATATGGG CCTCCGGTGG CCATATGGAC
    CAAATGATGT TTTCTGATCA TAGCACAAAA TATAACAGAC AGAATCAAAG TAGAGAGAGT
    CTTGAACAAG CCCAATCCCG GGCCAGCTGG GCGTCTTCCA CGGGCTACTG GGGAGAAGAC
    TCAGAAGGCG ACACAGGCAC TATCAAGCGA AGGGGTGGGA AGGATGTCTC CGCTGAGGCA
    GAGAGCAGCA GCATGGTGCC CGTGACTACA GAGGAAGCCA AACCTGTCCC TATGCCTGCC
    CACATAGCTG TGACGCCGAG CACTACCAAG GGACTCATCG CACGGAAGGA AGGCAGGTAC
    CGGGAGCCGC CTCCCACACC TCCAGGCTAC GTGGGCATCC CCATTGCCGA TTTCCCAGAA
    GGGCCTTGCC ACCCGGCCAG GAAGCCCCCG GATTACAACG TGGCCCTGCA GCGGTCCCGC
    ATGGTGGCAC GGCCCACTGA GGCCCCGGCA CCGGGCCAGA CGCCGCCTGC AGCCGCAGCC
    AGCCGGCCGG GCAGCAAGCC ACAGTGGCAC AAGCCCAGCG ACGCAGACCC ACGCCTCGCG
    CCCTTCCAGC CGCAGGGCTT CGCAGGAGCG GAGGAGGACG AAGATGAACA AGTGTCTGCT
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006502315.1
    CDS1320..5945
    Translation

    Target ORF information:

    RefSeq Version XM_006502252.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006502252.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TCCAACGTCA AGCCTCCCAC 
    AGACAGTCAC GGAGAAGATT TAGAAAGATC AACCAGAAAG GTGAAAGACA AACAATTATC
    GACACAGTGG ACCCTTACCC TGTGGGCAAG CCACCTTTAC CTAGAGGCTA CCATACGGAA
    TGCACTAAAT CTCAGCTCCC TGCAGACTTC ACAAAACTGC ACCTCACTGA CAGCCTGCAC
    CCACAGGTGA CCCACGTGTC CTCCAGCCAT TCAGGATGTA GCATCACCAG CGACTCTGGG
    AGCAGCAGTC TTTCTGACAT TTACCAGGCC ACGGAGAGCG AGGCCGGTGA CATGGACCTG
    AGTGGACTGC CAGAGACGGC GGTTGACTCT GAGGATGACG ATGATGAGGA AGACATCGAG
    AGAGCATCAG ACCCTCTGAT GAGCAGGGAC ATCGTGAGGG ACTGCCTGGA GAAGGACCCG
    ATAGACAGGA CTGACGATGA CATCGAACAA CTACTGGAAT TTATGCACCA GCTGCCGGCC
    TTTGCCAATA TGACGATGTC AGTAAGACGG GAGCTCTGTG CCGTGATGGT GTTTGCAGTG
    GTGGAAAGAG CTGGAACCAT TGTGTTAAAC GACGGAGAAG AGCTGGATTC CTGGTCAGTG
    ATTCTCAATG GTTCCGTGGA GGTGACATAT CCGGATGGGA AAGCAGAAAT TCTGTGCATG
    GGAAACAGCT TTGGTGTTTC GCCCACCATG GACAAAGAAT ACATGAAAGG AGTCATGAGG
    ACTAAGGTGG ATGACTGCCA GTTTGTCTGC ATTGCCCAGC AAGATTACTG CCGTATTTTG
    AACCAAGTAG AAAAGAACAT GCAAAAAGTT GAGGAGGAAG GAGAGATTGT AATGGTGAAG
    GAGCACCGAG AACTTGATCG GACTGGAACA AGGAAGGGCC ACATCGTCAT CAAGGGCACC
    TCAGAGAGGC TGACCATGCA TTTGGTGGAA GAGCATTCTG TAGTGGATCC AACATTCATA
    GAAGACTTCC TGTTGACATA CCGGACTTTC CTTTCCAGCC CGATGGAAGT GGGCAAGAAG
    TTATTGGAGT GGTTCAATGA CCCGAGCCTC AGGGATAAGG TGACGCGGGT AGTATTACTG
    TGGGTGAATA ATCACTTCAA TGACTTTGAA GGGGATCCTG CAATGACTCG ATTTCTGGAA
    GAATTTGAAA ATAATCTGGA AAGAGAGAAA ATGGGTGGAC ATCTAAGGCT GTTGAACATT
    GCATGTGCAG CTAAAGCCAA ACGAAGACTG ATGACGTTAA CAAAGCCTTC CCGGGAAGCC
    CCTCTGCCCT TCATCTTACT GGGAGGCTCT GAAAAGGGCT TTGGCATCTT CGTGGACAGC
    GTTGACTCGT GTAGCAAAGC CACTGAAGCA GGCTTGAAGC GCGGGGATCA GATATTAGAA
    GTGAATGGTC AAAATTTTGA AAATATCCAA CTTTCAAAGG CAATGGAAAT TCTCAGAAAT
    AACACACACC TGTCCATCAC TGTGAAAACC AACTTATTTG TATTTAAAGA ACTTCTGACG
    AGATTGTCTG AAGAGAAAAG AAACGGTGCC CCTCACCTCC CTAAAATCGG GGACATCAAG
    AAGGCCAGCC GCTACTCCAT ACCGGATCTC GCCGTAGACG TAGAACAGGT GATAGGCCTC
    GAGAAGGTCA ACAAGAAAAG CAAAGCCAAC ACCGTGGGAG GAAGAAACAA GCTGAAGAAA
    ATACTCGACA AGACCCGGAT CAGCATCTTA CCTCAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGATAG CATCGTAGGA CTGAGGCAGA CCAAGCACAT CCCAGCTGCC
    CTGCCTGTCA GTGGAACGCT GTCCTCCAGT AACCCTGACT TGCTGCAGTC ACACCACCGG
    ATTCTGGACT TCAGCACTAC TCCTGACTTG CCAGATCAGG TACTGAGAGT TTTCAAGGCT
    GACCAACAAA GCAGGTACAT CATGATCAGC AAGGACACCA CCGCAAAGGA AGTGGTCATC
    CAGGCCATCA GAGAGTTTGC TGTCACTGCC ACCCCAGAGC AGTATTCCCT CTGTGAGGTC
    TCAGTCACAC CAGAGGGAGT TATCAAACAA CGCCGACTCC CAGATCAGCT TTCCAAGCTT
    GCCGACAGGA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACG
    CTTTGCTCAG ATGAAGATGC CCAGGAGCTG CTGCGAGAGA GCCAAATCTC CCTCCTTCAG
    CTCAGCACCG TGGAGGTTGC CACGCAGCTC TCCATGAGGA ACTTTGAACT CTTCCGCAAC
    ATTGAGCCTA CTGAATACAT AGATGATTTA TTTAAACTCA AATCAAAAAC CAGCTGTGCC
    AACCTGAAGA AATTTGAGGA AGTCATTAAC CAGGAAACAT TTTGGGTGGC TTCTGAAATC
    CTGCGGGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATTGCC
    TTGCACTGCA GGGAATGCAA GAATTTTAAC TCCATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCGG TGGCAAGACT ACGAACCACC TGGGAAAAGC TTCCCAATAA ATATGAAAAG
    CTGTTCCAGG ACCTGCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA GTACCGCAAT
    GTCCTGAGTG GCCAGAACTT ACAGCCTCCT GTCATTCCCC TGTTCCCTGT CATCAAGAAG
    GACCTCACGT TTCTCCATGA GGGAAATGAC TCAAAAGTAG ACGGACTGGT CAACTTTGAG
    AAGCTGAGGA TGATTGCAAA AGAAATCCGT CACGTTGGCC GAATGGCATC AGTCAACATG
    GACCCTGCCC TTATGTTCAG GACTCGGAAG AAGAAGTGGC GGAGTCTGGG GTCCCTGAGT
    CAGGGCAGTG CAAACGCCAC AGTGCTGGAT GTTGCTCAGA CAGGCGGGCA TAAAAAGCGG
    GTACGCCGTA GTTCCTTCCT CAATGCCAAA AAGCTGTATG AAGATGCCCA GATGGCTCGG
    AAAGTGAAGC AATACCTGTC CAACCTGGAG CTGGAGATGG ATGAGGAGAG TCTTCAGACA
    CTGTCACTGC AGTGTGAACC AGCAACCAGC ACATTGCCCA AGAATCCTGG AGACAAAAAG
    CCTGTCAAGT CTGAGACCTC CCCAGTGGCT CCACGTGCAG GGCCCCAGCA GAAAGTACAG
    CCTCAGCAGC CGCTGGCACA GCCACAACCT CCACATAAAG TCAGCCAGGG ACTGCAGGTG
    CCCGCTGTGT CCCTCTATCC TTCACGAAAG AAGGTGCCGG TGAAGGATCT GCCTCCTTTT
    GGCATAAACT CTCCACAAGC TTTGAAGAAA ATCCTGTCCT TGTCTGAAGA AGGGAGTTTG
    GAGCGTCACA GGAAGCAAGC AGAAGATACA ATATCAAATG CGTCCTCTCA GCTCTCATCG
    CCCCCCACCT CACCTCAGAG CTCTCCAAGG AAAGGTTATG CACTGGCTCT CAGTGGCACC
    GTGGATAATT TCTCAGATTC AGGTCACAGT GAGATTTCTT CACGATCCAG TATTGTCAGC
    AATTCTTCTT TTGACTCGGT GCCAGTCTCT CTGCATGATG AACGGCGCCA GAGGCATTCT
    GTCAGCATTG TGGAGAGCAA CCTAGGTGTG GGCAGGATGG AGCGGCGGAC CCTGATGGAG
    CCTGATCAAT ACAGCTTAGG GTCGTATGCC CCAGTGTCTG AAAGCCGAGG CCTGTATGCC
    GCAGCGACGG TCATCTCTTC TCCTAGCACA GAGGAGCTTT CCCATGACCA AGGGGACCGT
    GCTTCCCTCG ATGCTGCTGA CAGCGGTCGT GGGAGCTGGA CATCATGCTC AAGTGGTTCC
    CATGACAACA TACAGACCAT TCAGCACCAG AGAAGCTGGG AAACACTGCC ATTTGGGCAC
    ACTCACTTTG ACTATTCAGG GGATGCTGCA AGTATATGGG CCTCCGGTGG CCATATGGAC
    CAAATGATGT TTTCTGATCA TAGCACAAAA TATAACAGAC AGAATCAAAG TAGAGAGAGT
    CTTGAACAAG CCCAATCCCG GGCCAGCTGG GCGTCTTCCA CGGGCTACTG GGGAGAAGAC
    TCAGAAGGCG ACACAGGCAC TATCAAGCGA AGGGGTGGGA AGGATGTCTC CGCTGAGGCA
    GAGAGCAGCA GCATGGTGCC CGTGACTACA GAGGAAGCCA AACCTGTCCC TATGCCTGCC
    CACATAGCTG TGACGCCGAG CACTACCAAG GGACTCATCG CACGGAAGGA AGGCAGGTAC
    CGGGAGCCGC CTCCCACACC TCCAGGCTAC GTGGGCATCC CCATTGCCGA TTTCCCAGAA
    GGGCCTTGCC ACCCGGCCAG GAAGCCCCCG GATTACAACG TGGCCCTGCA GCGGTCCCGC
    ATGGTGGCAC GGCCCACTGA GGCCCCGGCA CCGGGCCAGA CGCCGCCTGC AGCCGCAGCC
    AGCCGGCCGG GCAGCAAGCC ACAGTGGCAC AAGCCCAGCG ACGCAGACCC ACGCCTCGCG
    CCCTTCCAGC CGCAGGGCTT CGCAGGAGCG GAGGAGGACG AAGATGAACA AGTGTCTGCT
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32400
    Clone ID Related Accession (Same CDS sequence) XM_006502254.3 , NM_001310536.2
    Accession Version XM_006502254.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502254.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TGGCCGCGCC TGCTAACCAT GGAGTTCTGG GCCAGCAGGA GAAGCAGTCT 
    CTCCCTGCAG ACTTCACAAA ACTGCACCTC ACTGACAGCC TGCACCCACA GGTGACCCAC
    GTGTCCTCCA GCCATTCAGG ATGTAGCATC ACCAGCGACT CTGGGAGCAG CAGTCTTTCT
    GACATTTACC AGGCCACGGA GAGCGAGGCC GGTGACATGG ACCTGAGTGG ACTGCCAGAG
    ACGGCGGTTG ACTCTGAGGA TGACGATGAT GAGGAAGACA TCGAGAGAGC ATCAGACCCT
    CTGATGAGCA GGGACATCGT GAGGGACTGC CTGGAGAAGG ACCCGATAGA CAGGACTGAC
    GATGACATCG AACAACTACT GGAATTTATG CACCAGCTGC CGGCCTTTGC CAATATGACG
    ATGTCAGTAA GACGGGAGCT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGA
    ACCATTGTGT TAAACGACGG AGAAGAGCTG GATTCCTGGT CAGTGATTCT CAATGGTTCC
    GTGGAGGTGA CATATCCGGA TGGGAAAGCA GAAATTCTGT GCATGGGAAA CAGCTTTGGT
    GTTTCGCCCA CCATGGACAA AGAATACATG AAAGGAGTCA TGAGGACTAA GGTGGATGAC
    TGCCAGTTTG TCTGCATTGC CCAGCAAGAT TACTGCCGTA TTTTGAACCA AGTAGAAAAG
    AACATGCAAA AAGTTGAGGA GGAAGGAGAG ATTGTAATGG TGAAGGAGCA CCGAGAACTT
    GATCGGACTG GAACAAGGAA GGGCCACATC GTCATCAAGG GCACCTCAGA GAGGCTGACC
    ATGCATTTGG TGGAAGAGCA TTCTGTAGTG GATCCAACAT TCATAGAAGA CTTCCTGTTG
    ACATACCGGA CTTTCCTTTC CAGCCCGATG GAAGTGGGCA AGAAGTTATT GGAGTGGTTC
    AATGACCCGA GCCTCAGGGA TAAGGTGACG CGGGTAGTAT TACTGTGGGT GAATAATCAC
    TTCAATGACT TTGAAGGGGA TCCTGCAATG ACTCGATTTC TGGAAGAATT TGAAAATAAT
    CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTGA ACATTGCATG TGCAGCTAAA
    GCCAAACGAA GACTGATGAC GTTAACAAAG CCTTCCCGGG AAGCCCCTCT GCCCTTCATC
    TTACTGGGAG GCTCTGAAAA GGGCTTTGGC ATCTTCGTGG ACAGCGTTGA CTCGTGTAGC
    AAAGCCACTG AAGCAGGCTT GAAGCGCGGG GATCAGATAT TAGAAGTGAA TGGTCAAAAT
    TTTGAAAATA TCCAACTTTC AAAGGCAATG GAAATTCTCA GAAATAACAC ACACCTGTCC
    ATCACTGTGA AAACCAACTT ATTTGTATTT AAAGAACTTC TGACGAGATT GTCTGAAGAG
    AAAAGAAACG GTGCCCCTCA CCTCCCTAAA ATCGGGGACA TCAAGAAGGC CAGCCGCTAC
    TCCATACCGG ATCTCGCCGT AGACGTAGAA CAGGTGATAG GCCTCGAGAA GGTCAACAAG
    AAAAGCAAAG CCAACACCGT GGGAGGAAGA AACAAGCTGA AGAAAATACT CGACAAGACC
    CGGATCAGCA TCTTACCTCA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GATAGCATCG TAGGACTGAG GCAGACCAAG CACATCCCAG CTGCCCTGCC TGTCAGTGGA
    ACGCTGTCCT CCAGTAACCC TGACTTGCTG CAGTCACACC ACCGGATTCT GGACTTCAGC
    ACTACTCCTG ACTTGCCAGA TCAGGTACTG AGAGTTTTCA AGGCTGACCA ACAAAGCAGG
    TACATCATGA TCAGCAAGGA CACCACCGCA AAGGAAGTGG TCATCCAGGC CATCAGAGAG
    TTTGCTGTCA CTGCCACCCC AGAGCAGTAT TCCCTCTGTG AGGTCTCAGT CACACCAGAG
    GGAGTTATCA AACAACGCCG ACTCCCAGAT CAGCTTTCCA AGCTTGCCGA CAGGATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACGCTTTG CTCAGATGAA
    GATGCCCAGG AGCTGCTGCG AGAGAGCCAA ATCTCCCTCC TTCAGCTCAG CACCGTGGAG
    GTTGCCACGC AGCTCTCCAT GAGGAACTTT GAACTCTTCC GCAACATTGA GCCTACTGAA
    TACATAGATG ATTTATTTAA ACTCAAATCA AAAACCAGCT GTGCCAACCT GAAGAAATTT
    GAGGAAGTCA TTAACCAGGA AACATTTTGG GTGGCTTCTG AAATCCTGCG GGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCCTTGCA CTGCAGGGAA
    TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCGGTGGCA
    AGACTACGAA CCACCTGGGA AAAGCTTCCC AATAAATATG AAAAGCTGTT CCAGGACCTG
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAGTACC GCAATGTCCT GAGTGGCCAG
    AACTTACAGC CTCCTGTCAT TCCCCTGTTC CCTGTCATCA AGAAGGACCT CACGTTTCTC
    CATGAGGGAA ATGACTCAAA AGTAGACGGA CTGGTCAACT TTGAGAAGCT GAGGATGATT
    GCAAAAGAAA TCCGTCACGT TGGCCGAATG GCATCAGTCA ACATGGACCC TGCCCTTATG
    TTCAGGACTC GGAAGAAGAA GTGGCGGAGT CTGGGGTCCC TGAGTCAGGG CAGTGCAAAC
    GCCACAGTGC TGGATGTTGC TCAGACAGGC GGGCATAAAA AGCGGGTACG CCGTAGTTCC
    TTCCTCAATG CCAAAAAGCT GTATGAAGAT GCCCAGATGG CTCGGAAAGT GAAGCAATAC
    CTGTCCAACC TGGAGCTGGA GATGGATGAG GAGAGTCTTC AGACACTGTC ACTGCAGTGT
    GAACCAGCAA CCAGCACATT GCCCAAGAAT CCTGGAGACA AAAAGCCTGT CAAGTCTGAG
    ACCTCCCCAG TGGCTCCACG TGCAGGGCCC CAGCAGAAAG TACAGCCTCA GCAGCCGCTG
    GCACAGCCAC AACCTCCACA TAAAGTCAGC CAGGGACTGC AGGTGCCCGC TGTGTCCCTC
    TATCCTTCAC GAAAGAAGGT GCCGGTGAAG GATCTGCCTC CTTTTGGCAT AAACTCTCCA
    CAAGCTTTGA AGAAAATCCT GTCCTTGTCT GAAGAAGGGA GTTTGGAGCG TCACAGGAAG
    CAAGCAGAAG ATACAATATC AAATGCGTCC TCTCAGCTCT CATCGCCCCC CACCTCACCT
    CAGAGCTCTC CAAGGAAAGG TTATGCACTG GCTCTCAGTG GCACCGTGGA TAATTTCTCA
    GATTCAGGTC ACAGTGAGAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCTTTTGAC
    TCGGTGCCAG TCTCTCTGCA TGATGAACGG CGCCAGAGGC ATTCTGTCAG CATTGTGGAG
    AGCAACCTAG GTGTGGGCAG GATGGAGCGG CGGACCCTGA TGGAGCCTGA TCAATACAGC
    TTAGGGTCGT ATGCCCCAGT GTCTGAAAGC CGAGGCCTGT ATGCCGCAGC GACGGTCATC
    TCTTCTCCTA GCACAGAGGA GCTTTCCCAT GACCAAGGGG ACCGTGCTTC CCTCGATGCT
    GCTGACAGCG GTCGTGGGAG CTGGACATCA TGCTCAAGTG GTTCCCATGA CAACATACAG
    ACCATTCAGC ACCAGAGAAG CTGGGAAACA CTGCCATTTG GGCACACTCA CTTTGACTAT
    TCAGGGGATG CTGCAAGTAT ATGGGCCTCC GGTGGCCATA TGGACCAAAT GATGTTTTCT
    GATCATAGCA CAAAATATAA CAGACAGAAT CAAAGTAGAG AGAGTCTTGA ACAAGCCCAA
    TCCCGGGCCA GCTGGGCGTC TTCCACGGGC TACTGGGGAG AAGACTCAGA AGGCGACACA
    GGCACTATCA AGCGAAGGGG TGGGAAGGAT GTCTCCGCTG AGGCAGAGAG CAGCAGCATG
    GTGCCCGTGA CTACAGAGGA AGCCAAACCT GTCCCTATGC CTGCCCACAT AGCTGTGACG
    CCGAGCACTA CCAAGGGACT CATCGCACGG AAGGAAGGCA GGTACCGGGA GCCGCCTCCC
    ACACCTCCAG GCTACGTGGG CATCCCCATT GCCGATTTCC CAGAAGGGCC TTGCCACCCG
    GCCAGGAAGC CCCCGGATTA CAACGTGGCC CTGCAGCGGT CCCGCATGGT GGCACGGCCC
    ACTGAGGCCC CGGCACCGGG CCAGACGCCG CCTGCAGCCG CAGCCAGCCG GCCGGGCAGC
    AAGCCACAGT GGCACAAGCC CAGCGACGCA GACCCACGCC TCGCGCCCTT CCAGCCGCAG
    GGCTTCGCAG GAGCGGAGGA GGACGAAGAT GAACAAGTGT CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006502317.1
    CDS832..5322
    Translation

    Target ORF information:

    RefSeq Version XM_006502254.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006502254.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TGGCCGCGCC TGCTAACCAT GGAGTTCTGG GCCAGCAGGA GAAGCAGTCT 
    CTCCCTGCAG ACTTCACAAA ACTGCACCTC ACTGACAGCC TGCACCCACA GGTGACCCAC
    GTGTCCTCCA GCCATTCAGG ATGTAGCATC ACCAGCGACT CTGGGAGCAG CAGTCTTTCT
    GACATTTACC AGGCCACGGA GAGCGAGGCC GGTGACATGG ACCTGAGTGG ACTGCCAGAG
    ACGGCGGTTG ACTCTGAGGA TGACGATGAT GAGGAAGACA TCGAGAGAGC ATCAGACCCT
    CTGATGAGCA GGGACATCGT GAGGGACTGC CTGGAGAAGG ACCCGATAGA CAGGACTGAC
    GATGACATCG AACAACTACT GGAATTTATG CACCAGCTGC CGGCCTTTGC CAATATGACG
    ATGTCAGTAA GACGGGAGCT CTGTGCCGTG ATGGTGTTTG CAGTGGTGGA AAGAGCTGGA
    ACCATTGTGT TAAACGACGG AGAAGAGCTG GATTCCTGGT CAGTGATTCT CAATGGTTCC
    GTGGAGGTGA CATATCCGGA TGGGAAAGCA GAAATTCTGT GCATGGGAAA CAGCTTTGGT
    GTTTCGCCCA CCATGGACAA AGAATACATG AAAGGAGTCA TGAGGACTAA GGTGGATGAC
    TGCCAGTTTG TCTGCATTGC CCAGCAAGAT TACTGCCGTA TTTTGAACCA AGTAGAAAAG
    AACATGCAAA AAGTTGAGGA GGAAGGAGAG ATTGTAATGG TGAAGGAGCA CCGAGAACTT
    GATCGGACTG GAACAAGGAA GGGCCACATC GTCATCAAGG GCACCTCAGA GAGGCTGACC
    ATGCATTTGG TGGAAGAGCA TTCTGTAGTG GATCCAACAT TCATAGAAGA CTTCCTGTTG
    ACATACCGGA CTTTCCTTTC CAGCCCGATG GAAGTGGGCA AGAAGTTATT GGAGTGGTTC
    AATGACCCGA GCCTCAGGGA TAAGGTGACG CGGGTAGTAT TACTGTGGGT GAATAATCAC
    TTCAATGACT TTGAAGGGGA TCCTGCAATG ACTCGATTTC TGGAAGAATT TGAAAATAAT
    CTGGAAAGAG AGAAAATGGG TGGACATCTA AGGCTGTTGA ACATTGCATG TGCAGCTAAA
    GCCAAACGAA GACTGATGAC GTTAACAAAG CCTTCCCGGG AAGCCCCTCT GCCCTTCATC
    TTACTGGGAG GCTCTGAAAA GGGCTTTGGC ATCTTCGTGG ACAGCGTTGA CTCGTGTAGC
    AAAGCCACTG AAGCAGGCTT GAAGCGCGGG GATCAGATAT TAGAAGTGAA TGGTCAAAAT
    TTTGAAAATA TCCAACTTTC AAAGGCAATG GAAATTCTCA GAAATAACAC ACACCTGTCC
    ATCACTGTGA AAACCAACTT ATTTGTATTT AAAGAACTTC TGACGAGATT GTCTGAAGAG
    AAAAGAAACG GTGCCCCTCA CCTCCCTAAA ATCGGGGACA TCAAGAAGGC CAGCCGCTAC
    TCCATACCGG ATCTCGCCGT AGACGTAGAA CAGGTGATAG GCCTCGAGAA GGTCAACAAG
    AAAAGCAAAG CCAACACCGT GGGAGGAAGA AACAAGCTGA AGAAAATACT CGACAAGACC
    CGGATCAGCA TCTTACCTCA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GATAGCATCG TAGGACTGAG GCAGACCAAG CACATCCCAG CTGCCCTGCC TGTCAGTGGA
    ACGCTGTCCT CCAGTAACCC TGACTTGCTG CAGTCACACC ACCGGATTCT GGACTTCAGC
    ACTACTCCTG ACTTGCCAGA TCAGGTACTG AGAGTTTTCA AGGCTGACCA ACAAAGCAGG
    TACATCATGA TCAGCAAGGA CACCACCGCA AAGGAAGTGG TCATCCAGGC CATCAGAGAG
    TTTGCTGTCA CTGCCACCCC AGAGCAGTAT TCCCTCTGTG AGGTCTCAGT CACACCAGAG
    GGAGTTATCA AACAACGCCG ACTCCCAGAT CAGCTTTCCA AGCTTGCCGA CAGGATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACGCTTTG CTCAGATGAA
    GATGCCCAGG AGCTGCTGCG AGAGAGCCAA ATCTCCCTCC TTCAGCTCAG CACCGTGGAG
    GTTGCCACGC AGCTCTCCAT GAGGAACTTT GAACTCTTCC GCAACATTGA GCCTACTGAA
    TACATAGATG ATTTATTTAA ACTCAAATCA AAAACCAGCT GTGCCAACCT GAAGAAATTT
    GAGGAAGTCA TTAACCAGGA AACATTTTGG GTGGCTTCTG AAATCCTGCG GGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TTGCCTTGCA CTGCAGGGAA
    TGCAAGAATT TTAACTCCAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCGGTGGCA
    AGACTACGAA CCACCTGGGA AAAGCTTCCC AATAAATATG AAAAGCTGTT CCAGGACCTG
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAGTACC GCAATGTCCT GAGTGGCCAG
    AACTTACAGC CTCCTGTCAT TCCCCTGTTC CCTGTCATCA AGAAGGACCT CACGTTTCTC
    CATGAGGGAA ATGACTCAAA AGTAGACGGA CTGGTCAACT TTGAGAAGCT GAGGATGATT
    GCAAAAGAAA TCCGTCACGT TGGCCGAATG GCATCAGTCA ACATGGACCC TGCCCTTATG
    TTCAGGACTC GGAAGAAGAA GTGGCGGAGT CTGGGGTCCC TGAGTCAGGG CAGTGCAAAC
    GCCACAGTGC TGGATGTTGC TCAGACAGGC GGGCATAAAA AGCGGGTACG CCGTAGTTCC
    TTCCTCAATG CCAAAAAGCT GTATGAAGAT GCCCAGATGG CTCGGAAAGT GAAGCAATAC
    CTGTCCAACC TGGAGCTGGA GATGGATGAG GAGAGTCTTC AGACACTGTC ACTGCAGTGT
    GAACCAGCAA CCAGCACATT GCCCAAGAAT CCTGGAGACA AAAAGCCTGT CAAGTCTGAG
    ACCTCCCCAG TGGCTCCACG TGCAGGGCCC CAGCAGAAAG TACAGCCTCA GCAGCCGCTG
    GCACAGCCAC AACCTCCACA TAAAGTCAGC CAGGGACTGC AGGTGCCCGC TGTGTCCCTC
    TATCCTTCAC GAAAGAAGGT GCCGGTGAAG GATCTGCCTC CTTTTGGCAT AAACTCTCCA
    CAAGCTTTGA AGAAAATCCT GTCCTTGTCT GAAGAAGGGA GTTTGGAGCG TCACAGGAAG
    CAAGCAGAAG ATACAATATC AAATGCGTCC TCTCAGCTCT CATCGCCCCC CACCTCACCT
    CAGAGCTCTC CAAGGAAAGG TTATGCACTG GCTCTCAGTG GCACCGTGGA TAATTTCTCA
    GATTCAGGTC ACAGTGAGAT TTCTTCACGA TCCAGTATTG TCAGCAATTC TTCTTTTGAC
    TCGGTGCCAG TCTCTCTGCA TGATGAACGG CGCCAGAGGC ATTCTGTCAG CATTGTGGAG
    AGCAACCTAG GTGTGGGCAG GATGGAGCGG CGGACCCTGA TGGAGCCTGA TCAATACAGC
    TTAGGGTCGT ATGCCCCAGT GTCTGAAAGC CGAGGCCTGT ATGCCGCAGC GACGGTCATC
    TCTTCTCCTA GCACAGAGGA GCTTTCCCAT GACCAAGGGG ACCGTGCTTC CCTCGATGCT
    GCTGACAGCG GTCGTGGGAG CTGGACATCA TGCTCAAGTG GTTCCCATGA CAACATACAG
    ACCATTCAGC ACCAGAGAAG CTGGGAAACA CTGCCATTTG GGCACACTCA CTTTGACTAT
    TCAGGGGATG CTGCAAGTAT ATGGGCCTCC GGTGGCCATA TGGACCAAAT GATGTTTTCT
    GATCATAGCA CAAAATATAA CAGACAGAAT CAAAGTAGAG AGAGTCTTGA ACAAGCCCAA
    TCCCGGGCCA GCTGGGCGTC TTCCACGGGC TACTGGGGAG AAGACTCAGA AGGCGACACA
    GGCACTATCA AGCGAAGGGG TGGGAAGGAT GTCTCCGCTG AGGCAGAGAG CAGCAGCATG
    GTGCCCGTGA CTACAGAGGA AGCCAAACCT GTCCCTATGC CTGCCCACAT AGCTGTGACG
    CCGAGCACTA CCAAGGGACT CATCGCACGG AAGGAAGGCA GGTACCGGGA GCCGCCTCCC
    ACACCTCCAG GCTACGTGGG CATCCCCATT GCCGATTTCC CAGAAGGGCC TTGCCACCCG
    GCCAGGAAGC CCCCGGATTA CAACGTGGCC CTGCAGCGGT CCCGCATGGT GGCACGGCCC
    ACTGAGGCCC CGGCACCGGG CCAGACGCCG CCTGCAGCCG CAGCCAGCCG GCCGGGCAGC
    AAGCCACAGT GGCACAAGCC CAGCGACGCA GACCCACGCC TCGCGCCCTT CCAGCCGCAG
    GGCTTCGCAG GAGCGGAGGA GGACGAAGAT GAACAAGTGT CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu71469
    Clone ID Related Accession (Same CDS sequence) XM_011240276.3 , XM_011240276.2
    Accession Version XM_011240276.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGGAATG CACTAAATCT
    CAGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGAAGAA GAAGTGGCGG AGTCTGGGGT CCCTGAGTCA GGGCAGTGCA
    AACGCCACAG TGCTGGATGT TGCTCAGACA GGCGGGCATA AAAAGCGGGT ACGCCGTAGT
    TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGGAA AGTGAAGCAA
    TACCTGTCCA ACCTGGAGCT GGAGATGGAT GAGGAGAGTC TTCAGACACT GTCACTGCAG
    TGTGAACCAG CAACCAGCAC ATTGCCCAAG AATCCTGGAG ACAAAAAGCC TGTCAAGTCT
    GAGACCTCCC CAGTGGCTCC ACGTGCAGGG CCCCAGCAGA AAGTACAGCC TCAGCAGCCG
    CTGGCACAGC CACAACCTCC ACATAAAGTC AGCCAGGGAC TGCAGGTGCC CGCTGTGTCC
    CTCTATCCTT CACGAAAGAA GGTGCCGGTG AAGGATCTGC CTCCTTTTGG CATAAACTCT
    CCACAAGCTT TGAAGAAAAT CCTGTCCTTG TCTGAAGAAG GGAGTTTGGA GCGTCACAGG
    AAGCAAGCAG AAGATACAAT ATCAAATGCG TCCTCTCAGC TCTCATCGCC CCCCACCTCA
    CCTCAGAGCT CTCCAAGGAA AGGTTATGCA CTGGCTCTCA GTGGCACCGT GGATAATTTC
    TCAGATTCAG GTCACAGTGA GATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCTTTT
    GACTCGGTGC CAGTCTCTCT GCATGATGAA CGGCGCCAGA GGCATTCTGT CAGCATTGTG
    GAGAGCAACC TAGGTGTGGG CAGGATGGAG CGGCGGACCC TGATGGAGCC TGATCAATAC
    AGCTTAGGGT CGTATGCCCC AGTGTCTGAA AGCCGAGGCC TGTATGCCGC AGCGACGGTC
    ATCTCTTCTC CTAGCACAGA GGAGCTTTCC CATGACCAAG GGGACCGTGC TTCCCTCGAT
    GCTGCTGACA GCGGTCGTGG GAGCTGGACA TCATGCTCAA GTGGTTCCCA TGACAACATA
    CAGACCATTC AGCACCAGAG AAGCTGGGAA ACACTGCCAT TTGGGCACAC TCACTTTGAC
    TATTCAGGGG ATGCTGCAAG TATATGGGCC TCCGGTGGCC ATATGGACCA AATGATGTTT
    TCTGATCATA GCACAAAATA TAACAGACAG AATCAAAGTA GAGAGAGTCT TGAACAAGCC
    CAATCCCGGG CCAGCTGGGC GTCTTCCACG GGCTACTGGG GAGAAGACTC AGAAGGCGAC
    ACAGGCACTA TCAAGCGAAG GGGTGGGAAG GATGTCTCCG CTGAGGCAGA GAGCAGCAGC
    ATGGTGCCCG TGACTACAGA GGAAGCCAAA CCTGTCCCTA TGCCTGCCCA CATAGCTGTG
    ACGCCGAGCA CTACCAAGGG ACTCATCGCA CGGAAGGAAG GCAGGTACCG GGAGCCGCCT
    CCCACACCTC CAGGCTACGT GGGCATCCCC ATTGCCGATT TCCCAGAAGG GCCTTGCCAC
    CCGGCCAGGA AGCCCCCGGA TTACAACGTG GCCCTGCAGC GGTCCCGCAT GGTGGCACGG
    CCCACTGAGG CCCCGGCACC GGGCCAGACG CCGCCTGCAG CCGCAGCCAG CCGGCCGGGC
    AGCAAGCCAC AGTGGCACAA GCCCAGCGAC GCAGACCCAC GCCTCGCGCC CTTCCAGCCG
    CAGGGCTTCG CAGGAGCGGA GGAGGACGAA GATGAACAAG TGTCTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011238578.1
    CDS710..5683
    Translation

    Target ORF information:

    RefSeq Version XM_011240276.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011240276.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGGAATG CACTAAATCT
    CAGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGAAGAA GAAGTGGCGG AGTCTGGGGT CCCTGAGTCA GGGCAGTGCA
    AACGCCACAG TGCTGGATGT TGCTCAGACA GGCGGGCATA AAAAGCGGGT ACGCCGTAGT
    TCCTTCCTCA ATGCCAAAAA GCTGTATGAA GATGCCCAGA TGGCTCGGAA AGTGAAGCAA
    TACCTGTCCA ACCTGGAGCT GGAGATGGAT GAGGAGAGTC TTCAGACACT GTCACTGCAG
    TGTGAACCAG CAACCAGCAC ATTGCCCAAG AATCCTGGAG ACAAAAAGCC TGTCAAGTCT
    GAGACCTCCC CAGTGGCTCC ACGTGCAGGG CCCCAGCAGA AAGTACAGCC TCAGCAGCCG
    CTGGCACAGC CACAACCTCC ACATAAAGTC AGCCAGGGAC TGCAGGTGCC CGCTGTGTCC
    CTCTATCCTT CACGAAAGAA GGTGCCGGTG AAGGATCTGC CTCCTTTTGG CATAAACTCT
    CCACAAGCTT TGAAGAAAAT CCTGTCCTTG TCTGAAGAAG GGAGTTTGGA GCGTCACAGG
    AAGCAAGCAG AAGATACAAT ATCAAATGCG TCCTCTCAGC TCTCATCGCC CCCCACCTCA
    CCTCAGAGCT CTCCAAGGAA AGGTTATGCA CTGGCTCTCA GTGGCACCGT GGATAATTTC
    TCAGATTCAG GTCACAGTGA GATTTCTTCA CGATCCAGTA TTGTCAGCAA TTCTTCTTTT
    GACTCGGTGC CAGTCTCTCT GCATGATGAA CGGCGCCAGA GGCATTCTGT CAGCATTGTG
    GAGAGCAACC TAGGTGTGGG CAGGATGGAG CGGCGGACCC TGATGGAGCC TGATCAATAC
    AGCTTAGGGT CGTATGCCCC AGTGTCTGAA AGCCGAGGCC TGTATGCCGC AGCGACGGTC
    ATCTCTTCTC CTAGCACAGA GGAGCTTTCC CATGACCAAG GGGACCGTGC TTCCCTCGAT
    GCTGCTGACA GCGGTCGTGG GAGCTGGACA TCATGCTCAA GTGGTTCCCA TGACAACATA
    CAGACCATTC AGCACCAGAG AAGCTGGGAA ACACTGCCAT TTGGGCACAC TCACTTTGAC
    TATTCAGGGG ATGCTGCAAG TATATGGGCC TCCGGTGGCC ATATGGACCA AATGATGTTT
    TCTGATCATA GCACAAAATA TAACAGACAG AATCAAAGTA GAGAGAGTCT TGAACAAGCC
    CAATCCCGGG CCAGCTGGGC GTCTTCCACG GGCTACTGGG GAGAAGACTC AGAAGGCGAC
    ACAGGCACTA TCAAGCGAAG GGGTGGGAAG GATGTCTCCG CTGAGGCAGA GAGCAGCAGC
    ATGGTGCCCG TGACTACAGA GGAAGCCAAA CCTGTCCCTA TGCCTGCCCA CATAGCTGTG
    ACGCCGAGCA CTACCAAGGG ACTCATCGCA CGGAAGGAAG GCAGGTACCG GGAGCCGCCT
    CCCACACCTC CAGGCTACGT GGGCATCCCC ATTGCCGATT TCCCAGAAGG GCCTTGCCAC
    CCGGCCAGGA AGCCCCCGGA TTACAACGTG GCCCTGCAGC GGTCCCGCAT GGTGGCACGG
    CCCACTGAGG CCCCGGCACC GGGCCAGACG CCGCCTGCAG CCGCAGCCAG CCGGCCGGGC
    AGCAAGCCAC AGTGGCACAA GCCCAGCGAC GCAGACCCAC GCCTCGCGCC CTTCCAGCCG
    CAGGGCTTCG CAGGAGCGGA GGAGGACGAA GATGAACAAG TGTCTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu71470
    Clone ID Related Accession (Same CDS sequence) XM_011240277.2 , XM_011240277.3
    Accession Version XM_011240277.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGGAATG CACTAAATCT
    CAGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGTCCCT GAGTCAGGGC AGTGCAAACG CCACAGTGCT GGATGTTGCT
    CAGACAGGCG GGCATAAAAA GCGGGTACGC CGTAGTTCCT TCCTCAATGC CAAAAAGCTG
    TATGAAGATG CCCAGATGGC TCGGAAAGTG AAGCAATACC TGTCCAACCT GGAGCTGGAG
    ATGGATGAGG AGAGTCTTCA GACACTGTCA CTGCAGTGTG AACCAGCAAC CAGCACATTG
    CCCAAGAATC CTGGAGACAA AAAGCCTGTC AAGTCTGAGA CCTCCCCAGT GGCTCCACGT
    GCAGGGCCCC AGCAGAAAGT ACAGCCTCAG CAGCCGCTGG CACAGCCACA ACCTCCACAT
    AAAGTCAGCC AGGGACTGCA GGTGCCCGCT GTGTCCCTCT ATCCTTCACG AAAGAAGGTG
    CCGGTGAAGG ATCTGCCTCC TTTTGGCATA AACTCTCCAC AAGCTTTGAA GAAAATCCTG
    TCCTTGTCTG AAGAAGGGAG TTTGGAGCGT CACAGGAAGC AAGCAGAAGA TACAATATCA
    AATGCGTCCT CTCAGCTCTC ATCGCCCCCC ACCTCACCTC AGAGCTCTCC AAGGAAAGGT
    TATGCACTGG CTCTCAGTGG CACCGTGGAT AATTTCTCAG ATTCAGGTCA CAGTGAGATT
    TCTTCACGAT CCAGTATTGT CAGCAATTCT TCTTTTGACT CGGTGCCAGT CTCTCTGCAT
    GATGAACGGC GCCAGAGGCA TTCTGTCAGC ATTGTGGAGA GCAACCTAGG TGTGGGCAGG
    ATGGAGCGGC GGACCCTGAT GGAGCCTGAT CAATACAGCT TAGGGTCGTA TGCCCCAGTG
    TCTGAAAGCC GAGGCCTGTA TGCCGCAGCG ACGGTCATCT CTTCTCCTAG CACAGAGGAG
    CTTTCCCATG ACCAAGGGGA CCGTGCTTCC CTCGATGCTG CTGACAGCGG TCGTGGGAGC
    TGGACATCAT GCTCAAGTGG TTCCCATGAC AACATACAGA CCATTCAGCA CCAGAGAAGC
    TGGGAAACAC TGCCATTTGG GCACACTCAC TTTGACTATT CAGGGGATGC TGCAAGTATA
    TGGGCCTCCG GTGGCCATAT GGACCAAATG ATGTTTTCTG ATCATAGCAC AAAATATAAC
    AGACAGAATC AAAGTAGAGA GAGTCTTGAA CAAGCCCAAT CCCGGGCCAG CTGGGCGTCT
    TCCACGGGCT ACTGGGGAGA AGACTCAGAA GGCGACACAG GCACTATCAA GCGAAGGGGT
    GGGAAGGATG TCTCCGCTGA GGCAGAGAGC AGCAGCATGG TGCCCGTGAC TACAGAGGAA
    GCCAAACCTG TCCCTATGCC TGCCCACATA GCTGTGACGC CGAGCACTAC CAAGGGACTC
    ATCGCACGGA AGGAAGGCAG GTACCGGGAG CCGCCTCCCA CACCTCCAGG CTACGTGGGC
    ATCCCCATTG CCGATTTCCC AGAAGGGCCT TGCCACCCGG CCAGGAAGCC CCCGGATTAC
    AACGTGGCCC TGCAGCGGTC CCGCATGGTG GCACGGCCCA CTGAGGCCCC GGCACCGGGC
    CAGACGCCGC CTGCAGCCGC AGCCAGCCGG CCGGGCAGCA AGCCACAGTG GCACAAGCCC
    AGCGACGCAG ACCCACGCCT CGCGCCCTTC CAGCCGCAGG GCTTCGCAGG AGCGGAGGAG
    GACGAAGATG AACAAGTGTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011238579.1
    CDS710..5659
    Translation

    Target ORF information:

    RefSeq Version XM_011240277.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011240277.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTGGATAA TAGCTTCCGC CAAGCGGTGA TGAAGAACCC CGCCGAGAGG 
    ACCCCCCAGG ACCTGGAAAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTATCCAAC
    CTGAGGGAGC ACCAACTAAG GTTAATGTGC GAAACTGTGA GATATGAGAG ACACGAAGCT
    AATGAAGTTC TATACTATCC TGATGATATT GGCACCTGCT GGTATATACT TCTTTCTGGT
    TCCGTGTTCA TTAAGGAGTC CATGTTTCTT CCAAGGAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGCTTTA GGCGTGGCTG TGAATGCATT GTCTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTC CAACGTCAAG CCTCCCACAG ACAGTCACGG
    AGAAGATTTA GAAAGATCAA CCAGAAAGGT GAAAGACAAA CAATTATCGA CACAGTGGAC
    CCTTACCCTG TGGGCAAGCC ACCTTTACCT AGAGGCTACC ATACGGAATG CACTAAATCT
    CAGCTCCCTG CAGACTTCAC AAAACTGCAC CTCACTGACA GCCTGCACCC ACAGGTGACC
    CACGTGTCCT CCAGCCATTC AGGATGTAGC ATCACCAGCG ACTCTGGGAG CAGCAGTCTT
    TCTGACATTT ACCAGGCCAC GGAGAGCGAG GCCGGTGACA TGGACCTGAG TGGACTGCCA
    GAGACGGCGG TTGACTCTGA GGATGACGAT GATGAGGAAG ACATCGAGAG AGCATCAGAC
    CCTCTGATGA GCAGGGACAT CGTGAGGGAC TGCCTGGAGA AGGACCCGAT AGACAGGACT
    GACGATGACA TCGAACAACT ACTGGAATTT ATGCACCAGC TGCCGGCCTT TGCCAATATG
    ACGATGTCAG TAAGACGGGA GCTCTGTGCC GTGATGGTGT TTGCAGTGGT GGAAAGAGCT
    GGAACCATTG TGTTAAACGA CGGAGAAGAG CTGGATTCCT GGTCAGTGAT TCTCAATGGT
    TCCGTGGAGG TGACATATCC GGATGGGAAA GCAGAAATTC TGTGCATGGG AAACAGCTTT
    GGTGTTTCGC CCACCATGGA CAAAGAATAC ATGAAAGGAG TCATGAGGAC TAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT TGCCCAGCAA GATTACTGCC GTATTTTGAA CCAAGTAGAA
    AAGAACATGC AAAAAGTTGA GGAGGAAGGA GAGATTGTAA TGGTGAAGGA GCACCGAGAA
    CTTGATCGGA CTGGAACAAG GAAGGGCCAC ATCGTCATCA AGGGCACCTC AGAGAGGCTG
    ACCATGCATT TGGTGGAAGA GCATTCTGTA GTGGATCCAA CATTCATAGA AGACTTCCTG
    TTGACATACC GGACTTTCCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT ATTGGAGTGG
    TTCAATGACC CGAGCCTCAG GGATAAGGTG ACGCGGGTAG TATTACTGTG GGTGAATAAT
    CACTTCAATG ACTTTGAAGG GGATCCTGCA ATGACTCGAT TTCTGGAAGA ATTTGAAAAT
    AATCTGGAAA GAGAGAAAAT GGGTGGACAT CTAAGGCTGT TGAACATTGC ATGTGCAGCT
    AAAGCCAAAC GAAGACTGAT GACGTTAACA AAGCCTTCCC GGGAAGCCCC TCTGCCCTTC
    ATCTTACTGG GAGGCTCTGA AAAGGGCTTT GGCATCTTCG TGGACAGCGT TGACTCGTGT
    AGCAAAGCCA CTGAAGCAGG CTTGAAGCGC GGGGATCAGA TATTAGAAGT GAATGGTCAA
    AATTTTGAAA ATATCCAACT TTCAAAGGCA ATGGAAATTC TCAGAAATAA CACACACCTG
    TCCATCACTG TGAAAACCAA CTTATTTGTA TTTAAAGAAC TTCTGACGAG ATTGTCTGAA
    GAGAAAAGAA ACGGTGCCCC TCACCTCCCT AAAATCGGGG ACATCAAGAA GGCCAGCCGC
    TACTCCATAC CGGATCTCGC CGTAGACGTA GAACAGGTGA TAGGCCTCGA GAAGGTCAAC
    AAGAAAAGCA AAGCCAACAC CGTGGGAGGA AGAAACAAGC TGAAGAAAAT ACTCGACAAG
    ACCCGGATCA GCATCTTACC TCAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGATAGCA TCGTAGGACT GAGGCAGACC AAGCACATCC CAGCTGCCCT GCCTGTCAGT
    GGAACGCTGT CCTCCAGTAA CCCTGACTTG CTGCAGTCAC ACCACCGGAT TCTGGACTTC
    AGCACTACTC CTGACTTGCC AGATCAGGTA CTGAGAGTTT TCAAGGCTGA CCAACAAAGC
    AGGTACATCA TGATCAGCAA GGACACCACC GCAAAGGAAG TGGTCATCCA GGCCATCAGA
    GAGTTTGCTG TCACTGCCAC CCCAGAGCAG TATTCCCTCT GTGAGGTCTC AGTCACACCA
    GAGGGAGTTA TCAAACAACG CCGACTCCCA GATCAGCTTT CCAAGCTTGC CGACAGGATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACGCT TTGCTCAGAT
    GAAGATGCCC AGGAGCTGCT GCGAGAGAGC CAAATCTCCC TCCTTCAGCT CAGCACCGTG
    GAGGTTGCCA CGCAGCTCTC CATGAGGAAC TTTGAACTCT TCCGCAACAT TGAGCCTACT
    GAATACATAG ATGATTTATT TAAACTCAAA TCAAAAACCA GCTGTGCCAA CCTGAAGAAA
    TTTGAGGAAG TCATTAACCA GGAAACATTT TGGGTGGCTT CTGAAATCCT GCGGGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATTGCCTT GCACTGCAGG
    GAATGCAAGA ATTTTAACTC CATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCGGTG
    GCAAGACTAC GAACCACCTG GGAAAAGCTT CCCAATAAAT ATGAAAAGCT GTTCCAGGAC
    CTGCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAGT ACCGCAATGT CCTGAGTGGC
    CAGAACTTAC AGCCTCCTGT CATTCCCCTG TTCCCTGTCA TCAAGAAGGA CCTCACGTTT
    CTCCATGAGG GAAATGACTC AAAAGTAGAC GGACTGGTCA ACTTTGAGAA GCTGAGGATG
    ATTGCAAAAG AAATCCGTCA CGTTGGCCGA ATGGCATCAG TCAACATGGA CCCTGCCCTT
    ATGTTCAGGA CTCGGTCCCT GAGTCAGGGC AGTGCAAACG CCACAGTGCT GGATGTTGCT
    CAGACAGGCG GGCATAAAAA GCGGGTACGC CGTAGTTCCT TCCTCAATGC CAAAAAGCTG
    TATGAAGATG CCCAGATGGC TCGGAAAGTG AAGCAATACC TGTCCAACCT GGAGCTGGAG
    ATGGATGAGG AGAGTCTTCA GACACTGTCA CTGCAGTGTG AACCAGCAAC CAGCACATTG
    CCCAAGAATC CTGGAGACAA AAAGCCTGTC AAGTCTGAGA CCTCCCCAGT GGCTCCACGT
    GCAGGGCCCC AGCAGAAAGT ACAGCCTCAG CAGCCGCTGG CACAGCCACA ACCTCCACAT
    AAAGTCAGCC AGGGACTGCA GGTGCCCGCT GTGTCCCTCT ATCCTTCACG AAAGAAGGTG
    CCGGTGAAGG ATCTGCCTCC TTTTGGCATA AACTCTCCAC AAGCTTTGAA GAAAATCCTG
    TCCTTGTCTG AAGAAGGGAG TTTGGAGCGT CACAGGAAGC AAGCAGAAGA TACAATATCA
    AATGCGTCCT CTCAGCTCTC ATCGCCCCCC ACCTCACCTC AGAGCTCTCC AAGGAAAGGT
    TATGCACTGG CTCTCAGTGG CACCGTGGAT AATTTCTCAG ATTCAGGTCA CAGTGAGATT
    TCTTCACGAT CCAGTATTGT CAGCAATTCT TCTTTTGACT CGGTGCCAGT CTCTCTGCAT
    GATGAACGGC GCCAGAGGCA TTCTGTCAGC ATTGTGGAGA GCAACCTAGG TGTGGGCAGG
    ATGGAGCGGC GGACCCTGAT GGAGCCTGAT CAATACAGCT TAGGGTCGTA TGCCCCAGTG
    TCTGAAAGCC GAGGCCTGTA TGCCGCAGCG ACGGTCATCT CTTCTCCTAG CACAGAGGAG
    CTTTCCCATG ACCAAGGGGA CCGTGCTTCC CTCGATGCTG CTGACAGCGG TCGTGGGAGC
    TGGACATCAT GCTCAAGTGG TTCCCATGAC AACATACAGA CCATTCAGCA CCAGAGAAGC
    TGGGAAACAC TGCCATTTGG GCACACTCAC TTTGACTATT CAGGGGATGC TGCAAGTATA
    TGGGCCTCCG GTGGCCATAT GGACCAAATG ATGTTTTCTG ATCATAGCAC AAAATATAAC
    AGACAGAATC AAAGTAGAGA GAGTCTTGAA CAAGCCCAAT CCCGGGCCAG CTGGGCGTCT
    TCCACGGGCT ACTGGGGAGA AGACTCAGAA GGCGACACAG GCACTATCAA GCGAAGGGGT
    GGGAAGGATG TCTCCGCTGA GGCAGAGAGC AGCAGCATGG TGCCCGTGAC TACAGAGGAA
    GCCAAACCTG TCCCTATGCC TGCCCACATA GCTGTGACGC CGAGCACTAC CAAGGGACTC
    ATCGCACGGA AGGAAGGCAG GTACCGGGAG CCGCCTCCCA CACCTCCAGG CTACGTGGGC
    ATCCCCATTG CCGATTTCCC AGAAGGGCCT TGCCACCCGG CCAGGAAGCC CCCGGATTAC
    AACGTGGCCC TGCAGCGGTC CCGCATGGTG GCACGGCCCA CTGAGGCCCC GGCACCGGGC
    CAGACGCCGC CTGCAGCCGC AGCCAGCCGG CCGGGCAGCA AGCCACAGTG GCACAAGCCC
    AGCGACGCAG ACCCACGCCT CGCGCCCTTC CAGCCGCAGG GCTTCGCAGG AGCGGAGGAG
    GACGAAGATG AACAAGTGTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.