Dcp1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dcp1a
Entrez Gene ID	75901
Full Name	decapping mRNA 1A
Synonyms	1110066A22Rik,4930568L04Rik,AU019772,D14Ertd817e,Mitc1,SMIF
General protein information	Preferred Names decapping mRNA 1A Names mRNA-decapping enzyme 1A DCP1 decapping enzyme homolog A MAD homolog 4 interacting transcription coactivator 1 Smad4-interacting transcriptional co-activator decapping enzyme transcription factor SMIF
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 B\|14 18.75 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006519655.3	XP_006519718.1	mRNA-decapping enzyme 1A isoform X1
XM_006519655.4	XP_006519718.1	mRNA-decapping enzyme 1A isoform X1
XM_006519656.3	XP_006519719.1	mRNA-decapping enzyme 1A isoform X2
NM_133761.3	NP_598522.3	mRNA-decapping enzyme 1A
XM_006519656.2	XP_006519719.1	mRNA-decapping enzyme 1A isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dcp1a	NP_001178760.1
Xenopus tropicalis (tropical clawed frog)	dcp1a	XP_002943538.2
Homo sapiens (human)	DCP1A	NP_060873.4
Bos taurus (cattle)	DCP1A	NP_001095800.1
Canis lupus familiaris (dog)	DCP1A	XP_849483.2
Mus musculus (house mouse)	Dcp1a	NP_598522.3
Danio rerio (zebrafish)	dcp1a	NP_878313.2
Pan troglodytes (chimpanzee)	DCP1A	XP_001173059.1
	DCP1A	XP_001083614.2
Gallus gallus (chicken)	DCP1A	XP_004944623.1

Gene Ontology
Bibliography

Related articles in PubMed

The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline.
Bailey AS, Batista PJ, Gold RS, Chen YG, de Rooij DG, Chang HY, Fuller MT
eLife6(2017 Oct)

Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells.
Kato Y, Katsuki T, Kokubo H, Masuda A, Saga Y
Nature communications711272(2016 Apr)

Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development.
Suzuki A, Niimi Y, Shinmyozu K, Zhou Z, Kiso M, Saga Y
EMBO reports17(1)37-46(2016 Jan)

RNA Binding Protein Nanos2 Organizes Post-transcriptional Buffering System to Retain Primitive State of Mouse Spermatogonial Stem Cells.
Zhou Z, Shirakawa T, Ohbo K, Sada A, Wu Q, Hasegawa K, Saba R, Saga Y
Developmental cell34(1)96-107(2015 Jul)

Interaction of NANOS2 and NANOS3 with different components of the CNOT complex may contribute to the functional differences in mouse male germ cells.
Suzuki A, Niimi Y, Saga Y
Biology open3(12)1207-16(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PKR-induced translational inhibition appears to be specific to Dcp1a because the expression of other P-body components, Pan2, Pan3, Ccr4, or Caf1, did not result in the inhibition of poliovirus gene expression or induce eIF2alpha phosphorylation.
Title: mRNA decapping enzyme 1a (Dcp1a)-induced translational arrest through protein kinase R (PKR) activation requires the N-terminal enabled vasodilator-stimulated protein homology 1 (EVH1) domain.

The results showed that DCP1A and DCP2 are critical in the transition from mRNA stability to instability during meiotic maturation and that mRNA degradation must be successful to execute the oocyte-to-zygote transition.
Title: Maternally recruited DCP1A and DCP2 contribute to messenger RNA degradation during oocyte maturation and genome activation in mouse.

Recruitment of DCP1A and DCP2 increases the mRNA degradation capacity of the maturing oocyte so that by the 2-cell stage, most of the maternal mRNA is degraded.
Title: Losing mom's message: requirement for DCP1A and DCP2 in the degradation of maternal transcripts during oocyte maturation.

ERK-phosphorylated Dcp1a enhances its interaction with the decapping enzyme Dcp2 during early differentiation of 3T3-L1 cells.
Title: Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway during early differentiation of 3T3-L1 preadipocytes.

Data show the presence of P-body-like foci in mouse oocytes, as revealed by the presence of Dcp1a and the colocalization of RNA-associated protein 55 (RAP55) and the DEAD box RNA helicase Rck/p54.
Title: Dcp1-bodies in mouse oocytes.

The following Dcp1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dcp1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20891	NM_133761.3 Latest version!	Mus musculus decapping mRNA 1A (Dcp1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OMu50418	XM_006519655.4 Latest version!	Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu50419	XM_006519656.3 Latest version!	Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu50418	XM_006519655.3	Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu50419	XM_006519656.2	Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dcp1a ( NM_133761.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598522.3 Mus musculus decapping mRNA 1A (Dcp1a), mRNA.

Price & Availability↑

CloneID	OMu20891
Clone ID Related Accession (Same CDS sequence)	NM_133761.3
Accession Version	NM_133761.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	mRNA-decapping enzyme 1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV651307.1, AK016254.1, CK392647.1, CO429593.1 and AC119886.7. On Mar 20, 2007 this sequence version replaced NM_133761.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ344447.1, SRR1660817.56882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG AATCAGATGG GCGGCTCAAA TATCTCCAGT 
CCTGGGCTAC AGCCAAGCAC TCAGCTCTCT AATCTGGGAA GCACCGAGAC TCTAGAAGAG 
ACACCCTCTG GGTCACAGGA TAAGTCTGCT CCGTCTGGTC ATAAACATCT GACAGTAGAA 
GAGTTATTTG GAACCTCCTT GCCAAAGGAA CAACCAACAG CTATGGGTCT AGAGTCAGAG 
GACACAGACA AGCTGCTGGG AGATGCATCA CAGAAAGAGC CCAGCTCATT CCTCCCATTT 
CCCTTTGAGC AGTCAGGGGG AGCCCCTCAG TCTGAAAACC TGGGTATCCA TTCTGCCGCT 
CACCACACAG TTCAGCCTGA AGTCAGCACC CCAGTGCTGA TCACTCCCGC CTCCATTGCA 
CAGTCGGGTG ACAAACATCC CCCAAGCTAC ACACTCCCAC TGAGCCCTGT CCTCAGCCCC 
ACTCTGCCAG CTGAAGCTCC TACCACACAA GTTCCCCACT TACCTCGGAA TAGCACCATG 
ATACAAGCAG TAAAGACCAC ACCCAGGCAG AAATCTCCAC TCCTGAACCA GCCAGTCCCT 
GAGCTAAGCC ACAGCAGTCT GGTTGCTAGC CAGAGCCCCT TCAGAGCCCC GGTGAGCCTA 
GCAAACCCAG CTGGCACAGC CCTCCCAAGC GTTGATCTGC TTCAGAAACT CAGGTTGACC 
CCACAGCATG ACCAAATACA AGCACAGCCG CTTGGAAAAG GAACCATGGC GCCCAGCTTT 
TCTTCGGCAG CTGGCCAACT GGCCACCCCT GAGAGCTTCA TAGAGCCTTC TTCTAAGACA 
GCAGCAGCAA GAGCAGCAGT CTCAGCCTCC CTGAGCAACA TGGTGCTTGC TCCAACCCTC 
CAGTCTATGC AGCAAAACCA GGATCCTGAA GTATTTTCCC AGCCTAAGGT GTTACCCAGT 
GCCATCCCGA TTGCAGGCTC TCCATTGGTT CCTGCAACCA CCACAGCAGT ATCTTCAGTC 
CTGCTGTCCC CCAGTGTGTT CCAGCAAACG GTTCCCAGGG CCGCAGACCT AGAGAGGAAA 
GCCAGCTCCC CCTCTCCCCT CACTGTTGGG ACAGCAGAGA GTCAGAGAAA GCCTTCCATT 
ATCCTCAGCA AGTCTCAGCT CCAGGACACA CTGATACACC TAATAAAGAA TGACTCCAGC 
TTCCTCAGTA CGCTTCATGC AGTTTACTTG CAGGTTCTGA CCAAGAACAA AGACAACCAC 
AACCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598522.3
CDS	58..1866
Translation	MALSCSTVRPRRRGSALRSKMELLSRAEQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDQRPIDILEMLSRAKDEYERNQMGGSNISSPGLQPSTQLSNLGSTETLEETPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPTAMGLESEDTDKLLGDASQKEPSSFLPFPFEQSGGAPQSENLGIHSAAHHTVQPEVSTPVLITPASIAQSGDKHPPSYTLPLSPVLSPTLPAEAPTTQVPHLPRNSTMIQAVKTTPRQKSPLLNQPVPELSHSSLVASQSPFRAPVSLANPAGTALPSVDLLQKLRLTPQHDQIQAQPLGKGTMAPSFSSAAGQLATPESFIEPSSKTAAARAAVSASLSNMVLAPTLQSMQQNQDPEVFSQPKVLPSAIPIAGSPLVPATTTAVSSVLLSPSVFQQTVPRAADLERKASSPSPLTVGTAESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHAVYLQVLTKNKDNHNL

Target ORF information:

RefSeq Version	NM_133761.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus decapping mRNA 1A (Dcp1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133761.3

ORF Insert Sequence:

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG AATCAGATGG GCGGCTCAAA TATCTCCAGT 
CCTGGGCTAC AGCCAAGCAC TCAGCTCTCT AATCTGGGAA GCACCGAGAC TCTAGAAGAG 
ACACCCTCTG GGTCACAGGA TAAGTCTGCT CCGTCTGGTC ATAAACATCT GACAGTAGAA 
GAGTTATTTG GAACCTCCTT GCCAAAGGAA CAACCAACAG CTATGGGTCT AGAGTCAGAG 
GACACAGACA AGCTGCTGGG AGATGCATCA CAGAAAGAGC CCAGCTCATT CCTCCCATTT 
CCCTTTGAGC AGTCAGGGGG AGCCCCTCAG TCTGAAAACC TGGGTATCCA TTCTGCCGCT 
CACCACACAG TTCAGCCTGA AGTCAGCACC CCAGTGCTGA TCACTCCCGC CTCCATTGCA 
CAGTCGGGTG ACAAACATCC CCCAAGCTAC ACACTCCCAC TGAGCCCTGT CCTCAGCCCC 
ACTCTGCCAG CTGAAGCTCC TACCACACAA GTTCCCCACT TACCTCGGAA TAGCACCATG 
ATACAAGCAG TAAAGACCAC ACCCAGGCAG AAATCTCCAC TCCTGAACCA GCCAGTCCCT 
GAGCTAAGCC ACAGCAGTCT GGTTGCTAGC CAGAGCCCCT TCAGAGCCCC GGTGAGCCTA 
GCAAACCCAG CTGGCACAGC CCTCCCAAGC GTTGATCTGC TTCAGAAACT CAGGTTGACC 
CCACAGCATG ACCAAATACA AGCACAGCCG CTTGGAAAAG GAACCATGGC GCCCAGCTTT 
TCTTCGGCAG CTGGCCAACT GGCCACCCCT GAGAGCTTCA TAGAGCCTTC TTCTAAGACA 
GCAGCAGCAA GAGCAGCAGT CTCAGCCTCC CTGAGCAACA TGGTGCTTGC TCCAACCCTC 
CAGTCTATGC AGCAAAACCA GGATCCTGAA GTATTTTCCC AGCCTAAGGT GTTACCCAGT 
GCCATCCCGA TTGCAGGCTC TCCATTGGTT CCTGCAACCA CCACAGCAGT ATCTTCAGTC 
CTGCTGTCCC CCAGTGTGTT CCAGCAAACG GTTCCCAGGG CCGCAGACCT AGAGAGGAAA 
GCCAGCTCCC CCTCTCCCCT CACTGTTGGG ACAGCAGAGA GTCAGAGAAA GCCTTCCATT 
ATCCTCAGCA AGTCTCAGCT CCAGGACACA CTGATACACC TAATAAAGAA TGACTCCAGC 
TTCCTCAGTA CGCTTCATGC AGTTTACTTG CAGGTTCTGA CCAAGAACAA AGACAACCAC 
AACCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcp1a ( XM_006519655.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519718.1 Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50418
Clone ID Related Accession (Same CDS sequence)	XM_006519655.3 , XM_006519655.4
Accession Version	XM_006519655.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mRNA-decapping enzyme 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519655.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG AATCAGATGG GCGGCTCAAA TATCTCCAGT 
CCTGGGCTAC AGCCAAGCAC TCAGCTCTCT AATCTGGGAA GCACCGAGAC TCTAGAAGAG 
ACACCCTCTG GGTCACAGGA TAAGTCTGCT CCGTCTGGTC ATAAACATCT GACAGTAGAA 
GAGTTATTTG GAACCTCCTT GCCAAAGGAA CAACCAACAG CTATGGGTCT AGAGTCAGAG 
GACACAGACA AGCTGCTGGG AGATGCATCA CAGAAAGAGC CCAGCTCATT CCTCCCATTT 
CCCTTTGAGC AGTCAGGGGG AGCCCCTCAG TCTGAAAACC TGGGTATCCA TTCTGCCGCT 
CACCACACAG TTCAGCCTGA AGTCAGCACC CCAGTGCTGA TCACTCCCGC CTCCATTGCA 
CAGTCGGGTG ACAAACATCC CCCAAGCTAC ACACTCCCAC TGAGCCCTGT CCTCAGCCCC 
ACTCTGCCAG CTGAAGCTCC TACCACACAA GTTCCCCACT TACCTCGGAA TAGCACCATG 
ATACAAGCAG TAAAGACCAC ACCCAGGCAG AAATCTCCAC TCCTGAACCA GCCAGTCCCT 
GAGCTAAGCC ACAGCAGTCT GGTTGCTAGC CAGAGCCCCT TCAGAGCCCC GGTGAGCCTA 
GCAAACCCAG CTGGCACAGC CCTCCCAAGC GTTGATCTGC TTCAGAAACT CAGGTTGACC 
CCACAGCATG ACCAAATACA AGCACAGCCG CTTGGAAAAG GAACCATGGC GCCCAGCTTT 
TCTTCGGCAG CTGGCCAACT GGCCACCCCT GAGAGCTTCA TAGAGCCTTC TTCTAAGACA 
GCAGCAGCAA GAGCAGCAGT CTCAGCCTCC CTGAGCAACA TGGTGCTTGC TCCAACCCTC 
CAGATTGCAG GCTCTCCATT GGTTCCTGCA ACCACCACAG CAGTATCTTC AGTCCTGCTG 
TCCCCCAGTG TGTTCCAGCA AACGGTTCCC AGGGCCGCAG ACCTAGAGAG GAAAGCCAGC 
TCCCCCTCTC CCCTCACTGT TGGGACAGCA GAGAGTCAGA GAAAGCCTTC CATTATCCTC 
AGCAAGTCTC AGCTCCAGGA CACACTGATA CACCTAATAA AGAATGACTC CAGCTTCCTC 
AGTACGCTTC ATGCAGTTTA CTTGCAGGTT CTGACCAAGA ACAAAGACAA CCACAACCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519718.1
CDS	97..1839
Translation	MALSCSTVRPRRRGSALRSKMELLSRAEQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDQRPIDILEMLSRAKDEYERNQMGGSNISSPGLQPSTQLSNLGSTETLEETPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPTAMGLESEDTDKLLGDASQKEPSSFLPFPFEQSGGAPQSENLGIHSAAHHTVQPEVSTPVLITPASIAQSGDKHPPSYTLPLSPVLSPTLPAEAPTTQVPHLPRNSTMIQAVKTTPRQKSPLLNQPVPELSHSSLVASQSPFRAPVSLANPAGTALPSVDLLQKLRLTPQHDQIQAQPLGKGTMAPSFSSAAGQLATPESFIEPSSKTAAARAAVSASLSNMVLAPTLQIAGSPLVPATTTAVSSVLLSPSVFQQTVPRAADLERKASSPSPLTVGTAESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHAVYLQVLTKNKDNHNL

Target ORF information:

RefSeq Version	XM_006519655.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519655.3

ORF Insert Sequence:

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG AATCAGATGG GCGGCTCAAA TATCTCCAGT 
CCTGGGCTAC AGCCAAGCAC TCAGCTCTCT AATCTGGGAA GCACCGAGAC TCTAGAAGAG 
ACACCCTCTG GGTCACAGGA TAAGTCTGCT CCGTCTGGTC ATAAACATCT GACAGTAGAA 
GAGTTATTTG GAACCTCCTT GCCAAAGGAA CAACCAACAG CTATGGGTCT AGAGTCAGAG 
GACACAGACA AGCTGCTGGG AGATGCATCA CAGAAAGAGC CCAGCTCATT CCTCCCATTT 
CCCTTTGAGC AGTCAGGGGG AGCCCCTCAG TCTGAAAACC TGGGTATCCA TTCTGCCGCT 
CACCACACAG TTCAGCCTGA AGTCAGCACC CCAGTGCTGA TCACTCCCGC CTCCATTGCA 
CAGTCGGGTG ACAAACATCC CCCAAGCTAC ACACTCCCAC TGAGCCCTGT CCTCAGCCCC 
ACTCTGCCAG CTGAAGCTCC TACCACACAA GTTCCCCACT TACCTCGGAA TAGCACCATG 
ATACAAGCAG TAAAGACCAC ACCCAGGCAG AAATCTCCAC TCCTGAACCA GCCAGTCCCT 
GAGCTAAGCC ACAGCAGTCT GGTTGCTAGC CAGAGCCCCT TCAGAGCCCC GGTGAGCCTA 
GCAAACCCAG CTGGCACAGC CCTCCCAAGC GTTGATCTGC TTCAGAAACT CAGGTTGACC 
CCACAGCATG ACCAAATACA AGCACAGCCG CTTGGAAAAG GAACCATGGC GCCCAGCTTT 
TCTTCGGCAG CTGGCCAACT GGCCACCCCT GAGAGCTTCA TAGAGCCTTC TTCTAAGACA 
GCAGCAGCAA GAGCAGCAGT CTCAGCCTCC CTGAGCAACA TGGTGCTTGC TCCAACCCTC 
CAGATTGCAG GCTCTCCATT GGTTCCTGCA ACCACCACAG CAGTATCTTC AGTCCTGCTG 
TCCCCCAGTG TGTTCCAGCA AACGGTTCCC AGGGCCGCAG ACCTAGAGAG GAAAGCCAGC 
TCCCCCTCTC CCCTCACTGT TGGGACAGCA GAGAGTCAGA GAAAGCCTTC CATTATCCTC 
AGCAAGTCTC AGCTCCAGGA CACACTGATA CACCTAATAA AGAATGACTC CAGCTTCCTC 
AGTACGCTTC ATGCAGTTTA CTTGCAGGTT CTGACCAAGA ACAAAGACAA CCACAACCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcp1a ( XM_006519656.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519719.1 Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu50419
Clone ID Related Accession (Same CDS sequence)	XM_006519656.3 , XM_006519656.2
Accession Version	XM_006519656.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mRNA-decapping enzyme 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG TCTGCTCCGT CTGGTCATAA ACATCTGACA 
GTAGAAGAGT TATTTGGAAC CTCCTTGCCA AAGGAACAAC CAACAGCTAT GGGTCTAGAG 
TCAGAGGACA CAGACAAGCT GCTGGGAGAT GCATCACAGA AAGAGCCCAG CTCATTCCTC 
CCATTTCCCT TTGAGCAGTC AGGGGGAGCC CCTCAGTCTG AAAACCTGGG TATCCATTCT 
GCCGCTCACC ACACAGTTCA GCCTGAAGTC AGCACCCCAG TGCTGATCAC TCCCGCCTCC 
ATTGCACAGT CGGGTGACAA ACATCCCCCA AGCTACACAC TCCCACTGAG CCCTGTCCTC 
AGCCCCACTC TGCCAGCTGA AGCTCCTACC ACACAAGTTC CCCACTTACC TCGGAATAGC 
ACCATGATAC AAGCAGTAAA GACCACACCC AGGCAGAAAT CTCCACTCCT GAACCAGCCA 
GTCCCTGAGC TAAGCCACAG CAGTCTGGTT GCTAGCCAGA GCCCCTTCAG AGCCCCGGTG 
AGCCTAGCAA ACCCAGCTGG CACAGCCCTC CCAAGCGTTG ATCTGCTTCA GAAACTCAGG 
TTGACCCCAC AGCATGACCA AATACAAGCA CAGCCGCTTG GAAAAGGAAC CATGGCGCCC 
AGCTTTTCTT CGGCAGCTGG CCAACTGGCC ACCCCTGAGA GCTTCATAGA GCCTTCTTCT 
AAGACAGCAG CAGCAAGAGC AGCAGTCTCA GCCTCCCTGA GCAACATGGT GCTTGCTCCA 
ACCCTCCAGT CTATGCAGCA AAACCAGGAT CCTGAAGTAT TTTCCCAGCC TAAGGTGTTA 
CCCAGTGCCA TCCCGATTGC AGGCTCTCCA TTGGTTCCTG CAACCACCAC AGCAGTATCT 
TCAGTCCTGC TGTCCCCCAG TGTGTTCCAG CAAACGGTTC CCAGGGCCGC AGACCTAGAG 
AGGAAAGCCA GCTCCCCCTC TCCCCTCACT GTTGGGACAG CAGAGAGTCA GAGAAAGCCT 
TCCATTATCC TCAGCAAGTC TCAGCTCCAG GACACACTGA TACACCTAAT AAAGAATGAC 
TCCAGCTTCC TCAGTACGCT TCATGCAGTT TACTTGCAGG TTCTGACCAA GAACAAAGAC 
AACCACAACC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519719.1
CDS	96..1790
Translation	MALSCSTVRPRRRGSALRSKMELLSRAEQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDQRPIDILEMLSRAKDEYERSAPSGHKHLTVEELFGTSLPKEQPTAMGLESEDTDKLLGDASQKEPSSFLPFPFEQSGGAPQSENLGIHSAAHHTVQPEVSTPVLITPASIAQSGDKHPPSYTLPLSPVLSPTLPAEAPTTQVPHLPRNSTMIQAVKTTPRQKSPLLNQPVPELSHSSLVASQSPFRAPVSLANPAGTALPSVDLLQKLRLTPQHDQIQAQPLGKGTMAPSFSSAAGQLATPESFIEPSSKTAAARAAVSASLSNMVLAPTLQSMQQNQDPEVFSQPKVLPSAIPIAGSPLVPATTTAVSSVLLSPSVFQQTVPRAADLERKASSPSPLTVGTAESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHAVYLQVLTKNKDNHNL

Target ORF information:

RefSeq Version	XM_006519656.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519656.2

ORF Insert Sequence:

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG TCTGCTCCGT CTGGTCATAA ACATCTGACA 
GTAGAAGAGT TATTTGGAAC CTCCTTGCCA AAGGAACAAC CAACAGCTAT GGGTCTAGAG 
TCAGAGGACA CAGACAAGCT GCTGGGAGAT GCATCACAGA AAGAGCCCAG CTCATTCCTC 
CCATTTCCCT TTGAGCAGTC AGGGGGAGCC CCTCAGTCTG AAAACCTGGG TATCCATTCT 
GCCGCTCACC ACACAGTTCA GCCTGAAGTC AGCACCCCAG TGCTGATCAC TCCCGCCTCC 
ATTGCACAGT CGGGTGACAA ACATCCCCCA AGCTACACAC TCCCACTGAG CCCTGTCCTC 
AGCCCCACTC TGCCAGCTGA AGCTCCTACC ACACAAGTTC CCCACTTACC TCGGAATAGC 
ACCATGATAC AAGCAGTAAA GACCACACCC AGGCAGAAAT CTCCACTCCT GAACCAGCCA 
GTCCCTGAGC TAAGCCACAG CAGTCTGGTT GCTAGCCAGA GCCCCTTCAG AGCCCCGGTG 
AGCCTAGCAA ACCCAGCTGG CACAGCCCTC CCAAGCGTTG ATCTGCTTCA GAAACTCAGG 
TTGACCCCAC AGCATGACCA AATACAAGCA CAGCCGCTTG GAAAAGGAAC CATGGCGCCC 
AGCTTTTCTT CGGCAGCTGG CCAACTGGCC ACCCCTGAGA GCTTCATAGA GCCTTCTTCT 
AAGACAGCAG CAGCAAGAGC AGCAGTCTCA GCCTCCCTGA GCAACATGGT GCTTGCTCCA 
ACCCTCCAGT CTATGCAGCA AAACCAGGAT CCTGAAGTAT TTTCCCAGCC TAAGGTGTTA 
CCCAGTGCCA TCCCGATTGC AGGCTCTCCA TTGGTTCCTG CAACCACCAC AGCAGTATCT 
TCAGTCCTGC TGTCCCCCAG TGTGTTCCAG CAAACGGTTC CCAGGGCCGC AGACCTAGAG 
AGGAAAGCCA GCTCCCCCTC TCCCCTCACT GTTGGGACAG CAGAGAGTCA GAGAAAGCCT 
TCCATTATCC TCAGCAAGTC TCAGCTCCAG GACACACTGA TACACCTAAT AAAGAATGAC 
TCCAGCTTCC TCAGTACGCT TCATGCAGTT TACTTGCAGG TTCTGACCAA GAACAAAGAC 
AACCACAACC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcp1a ( XM_006519655.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519718.1 Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X1, mRNA.

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CloneID	OMu50418
Clone ID Related Accession (Same CDS sequence)	XM_006519655.3 , XM_006519655.4
Accession Version	XM_006519655.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mRNA-decapping enzyme 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006519655.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG AATCAGATGG GCGGCTCAAA TATCTCCAGT 
CCTGGGCTAC AGCCAAGCAC TCAGCTCTCT AATCTGGGAA GCACCGAGAC TCTAGAAGAG 
ACACCCTCTG GGTCACAGGA TAAGTCTGCT CCGTCTGGTC ATAAACATCT GACAGTAGAA 
GAGTTATTTG GAACCTCCTT GCCAAAGGAA CAACCAACAG CTATGGGTCT AGAGTCAGAG 
GACACAGACA AGCTGCTGGG AGATGCATCA CAGAAAGAGC CCAGCTCATT CCTCCCATTT 
CCCTTTGAGC AGTCAGGGGG AGCCCCTCAG TCTGAAAACC TGGGTATCCA TTCTGCCGCT 
CACCACACAG TTCAGCCTGA AGTCAGCACC CCAGTGCTGA TCACTCCCGC CTCCATTGCA 
CAGTCGGGTG ACAAACATCC CCCAAGCTAC ACACTCCCAC TGAGCCCTGT CCTCAGCCCC 
ACTCTGCCAG CTGAAGCTCC TACCACACAA GTTCCCCACT TACCTCGGAA TAGCACCATG 
ATACAAGCAG TAAAGACCAC ACCCAGGCAG AAATCTCCAC TCCTGAACCA GCCAGTCCCT 
GAGCTAAGCC ACAGCAGTCT GGTTGCTAGC CAGAGCCCCT TCAGAGCCCC GGTGAGCCTA 
GCAAACCCAG CTGGCACAGC CCTCCCAAGC GTTGATCTGC TTCAGAAACT CAGGTTGACC 
CCACAGCATG ACCAAATACA AGCACAGCCG CTTGGAAAAG GAACCATGGC GCCCAGCTTT 
TCTTCGGCAG CTGGCCAACT GGCCACCCCT GAGAGCTTCA TAGAGCCTTC TTCTAAGACA 
GCAGCAGCAA GAGCAGCAGT CTCAGCCTCC CTGAGCAACA TGGTGCTTGC TCCAACCCTC 
CAGATTGCAG GCTCTCCATT GGTTCCTGCA ACCACCACAG CAGTATCTTC AGTCCTGCTG 
TCCCCCAGTG TGTTCCAGCA AACGGTTCCC AGGGCCGCAG ACCTAGAGAG GAAAGCCAGC 
TCCCCCTCTC CCCTCACTGT TGGGACAGCA GAGAGTCAGA GAAAGCCTTC CATTATCCTC 
AGCAAGTCTC AGCTCCAGGA CACACTGATA CACCTAATAA AGAATGACTC CAGCTTCCTC 
AGTACGCTTC ATGCAGTTTA CTTGCAGGTT CTGACCAAGA ACAAAGACAA CCACAACCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519718.1
CDS	137..1879
Translation	MALSCSTVRPRRRGSALRSKMELLSRAEQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDQRPIDILEMLSRAKDEYERNQMGGSNISSPGLQPSTQLSNLGSTETLEETPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPTAMGLESEDTDKLLGDASQKEPSSFLPFPFEQSGGAPQSENLGIHSAAHHTVQPEVSTPVLITPASIAQSGDKHPPSYTLPLSPVLSPTLPAEAPTTQVPHLPRNSTMIQAVKTTPRQKSPLLNQPVPELSHSSLVASQSPFRAPVSLANPAGTALPSVDLLQKLRLTPQHDQIQAQPLGKGTMAPSFSSAAGQLATPESFIEPSSKTAAARAAVSASLSNMVLAPTLQIAGSPLVPATTTAVSSVLLSPSVFQQTVPRAADLERKASSPSPLTVGTAESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHAVYLQVLTKNKDNHNL

Target ORF information:

RefSeq Version	XM_006519655.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519655.4

ORF Insert Sequence:

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG AATCAGATGG GCGGCTCAAA TATCTCCAGT 
CCTGGGCTAC AGCCAAGCAC TCAGCTCTCT AATCTGGGAA GCACCGAGAC TCTAGAAGAG 
ACACCCTCTG GGTCACAGGA TAAGTCTGCT CCGTCTGGTC ATAAACATCT GACAGTAGAA 
GAGTTATTTG GAACCTCCTT GCCAAAGGAA CAACCAACAG CTATGGGTCT AGAGTCAGAG 
GACACAGACA AGCTGCTGGG AGATGCATCA CAGAAAGAGC CCAGCTCATT CCTCCCATTT 
CCCTTTGAGC AGTCAGGGGG AGCCCCTCAG TCTGAAAACC TGGGTATCCA TTCTGCCGCT 
CACCACACAG TTCAGCCTGA AGTCAGCACC CCAGTGCTGA TCACTCCCGC CTCCATTGCA 
CAGTCGGGTG ACAAACATCC CCCAAGCTAC ACACTCCCAC TGAGCCCTGT CCTCAGCCCC 
ACTCTGCCAG CTGAAGCTCC TACCACACAA GTTCCCCACT TACCTCGGAA TAGCACCATG 
ATACAAGCAG TAAAGACCAC ACCCAGGCAG AAATCTCCAC TCCTGAACCA GCCAGTCCCT 
GAGCTAAGCC ACAGCAGTCT GGTTGCTAGC CAGAGCCCCT TCAGAGCCCC GGTGAGCCTA 
GCAAACCCAG CTGGCACAGC CCTCCCAAGC GTTGATCTGC TTCAGAAACT CAGGTTGACC 
CCACAGCATG ACCAAATACA AGCACAGCCG CTTGGAAAAG GAACCATGGC GCCCAGCTTT 
TCTTCGGCAG CTGGCCAACT GGCCACCCCT GAGAGCTTCA TAGAGCCTTC TTCTAAGACA 
GCAGCAGCAA GAGCAGCAGT CTCAGCCTCC CTGAGCAACA TGGTGCTTGC TCCAACCCTC 
CAGATTGCAG GCTCTCCATT GGTTCCTGCA ACCACCACAG CAGTATCTTC AGTCCTGCTG 
TCCCCCAGTG TGTTCCAGCA AACGGTTCCC AGGGCCGCAG ACCTAGAGAG GAAAGCCAGC 
TCCCCCTCTC CCCTCACTGT TGGGACAGCA GAGAGTCAGA GAAAGCCTTC CATTATCCTC 
AGCAAGTCTC AGCTCCAGGA CACACTGATA CACCTAATAA AGAATGACTC CAGCTTCCTC 
AGTACGCTTC ATGCAGTTTA CTTGCAGGTT CTGACCAAGA ACAAAGACAA CCACAACCTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcp1a ( XM_006519656.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519719.1 Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu50419
Clone ID Related Accession (Same CDS sequence)	XM_006519656.3 , XM_006519656.2
Accession Version	XM_006519656.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mRNA-decapping enzyme 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006519656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG TCTGCTCCGT CTGGTCATAA ACATCTGACA 
GTAGAAGAGT TATTTGGAAC CTCCTTGCCA AAGGAACAAC CAACAGCTAT GGGTCTAGAG 
TCAGAGGACA CAGACAAGCT GCTGGGAGAT GCATCACAGA AAGAGCCCAG CTCATTCCTC 
CCATTTCCCT TTGAGCAGTC AGGGGGAGCC CCTCAGTCTG AAAACCTGGG TATCCATTCT 
GCCGCTCACC ACACAGTTCA GCCTGAAGTC AGCACCCCAG TGCTGATCAC TCCCGCCTCC 
ATTGCACAGT CGGGTGACAA ACATCCCCCA AGCTACACAC TCCCACTGAG CCCTGTCCTC 
AGCCCCACTC TGCCAGCTGA AGCTCCTACC ACACAAGTTC CCCACTTACC TCGGAATAGC 
ACCATGATAC AAGCAGTAAA GACCACACCC AGGCAGAAAT CTCCACTCCT GAACCAGCCA 
GTCCCTGAGC TAAGCCACAG CAGTCTGGTT GCTAGCCAGA GCCCCTTCAG AGCCCCGGTG 
AGCCTAGCAA ACCCAGCTGG CACAGCCCTC CCAAGCGTTG ATCTGCTTCA GAAACTCAGG 
TTGACCCCAC AGCATGACCA AATACAAGCA CAGCCGCTTG GAAAAGGAAC CATGGCGCCC 
AGCTTTTCTT CGGCAGCTGG CCAACTGGCC ACCCCTGAGA GCTTCATAGA GCCTTCTTCT 
AAGACAGCAG CAGCAAGAGC AGCAGTCTCA GCCTCCCTGA GCAACATGGT GCTTGCTCCA 
ACCCTCCAGT CTATGCAGCA AAACCAGGAT CCTGAAGTAT TTTCCCAGCC TAAGGTGTTA 
CCCAGTGCCA TCCCGATTGC AGGCTCTCCA TTGGTTCCTG CAACCACCAC AGCAGTATCT 
TCAGTCCTGC TGTCCCCCAG TGTGTTCCAG CAAACGGTTC CCAGGGCCGC AGACCTAGAG 
AGGAAAGCCA GCTCCCCCTC TCCCCTCACT GTTGGGACAG CAGAGAGTCA GAGAAAGCCT 
TCCATTATCC TCAGCAAGTC TCAGCTCCAG GACACACTGA TACACCTAAT AAAGAATGAC 
TCCAGCTTCC TCAGTACGCT TCATGCAGTT TACTTGCAGG TTCTGACCAA GAACAAAGAC 
AACCACAACC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519719.1
CDS	137..1831
Translation	MALSCSTVRPRRRGSALRSKMELLSRAEQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDQRPIDILEMLSRAKDEYERSAPSGHKHLTVEELFGTSLPKEQPTAMGLESEDTDKLLGDASQKEPSSFLPFPFEQSGGAPQSENLGIHSAAHHTVQPEVSTPVLITPASIAQSGDKHPPSYTLPLSPVLSPTLPAEAPTTQVPHLPRNSTMIQAVKTTPRQKSPLLNQPVPELSHSSLVASQSPFRAPVSLANPAGTALPSVDLLQKLRLTPQHDQIQAQPLGKGTMAPSFSSAAGQLATPESFIEPSSKTAAARAAVSASLSNMVLAPTLQSMQQNQDPEVFSQPKVLPSAIPIAGSPLVPATTTAVSSVLLSPSVFQQTVPRAADLERKASSPSPLTVGTAESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHAVYLQVLTKNKDNHNL

Target ORF information:

RefSeq Version	XM_006519656.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus decapping mRNA 1A (Dcp1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519656.3

ORF Insert Sequence:

ATGGCACTTT CCTGTTCCAC AGTCCGCCCC CGCCGGAGGG GCTCGGCTCT GAGATCCAAG 
ATGGAGTTGT TGAGTCGAGC TGAGCAAGAG ATGAGTCTGG CGGCCTTGAA ACAACACGAC 
CCCTATATCA CCAGCATCGC AGACCTCACG GGCCAGGTCG CCCTGTACAC TTTCTGCCCC 
AAAGCCAACC AGTGGGAGAA GACTGATATA GAAGGGACGT TATTTGTATA CCGAAGGTCA 
GCTTCGCCTT ACCATGGCTT TACCATTGTC AACCGACTGA ACATGCACAA TCTGGTTGAG 
CCAGTGAATA AAGATTTGGA ATTTCAGCTC CATGAACCAT TTCTTCTGTA TAGAAATGCA 
AGTTTGTCAA TATACAGTAT CTGGTTCTAT GACAAGAATG ACTGTCATCG CATAGCAAAA 
CTCATGGCTG ATGTGGTAGA AGAGGAGACA CGGCGATCCC AGCAGGCTGC TCGAGATAAA 
CAGAGTCCCA GCCAGGCCAA TGGCTGCAGC GACCAGAGGC CCATAGATAT CCTGGAGATG 
CTGAGCCGGG CCAAGGATGA GTATGAGAGG TCTGCTCCGT CTGGTCATAA ACATCTGACA 
GTAGAAGAGT TATTTGGAAC CTCCTTGCCA AAGGAACAAC CAACAGCTAT GGGTCTAGAG 
TCAGAGGACA CAGACAAGCT GCTGGGAGAT GCATCACAGA AAGAGCCCAG CTCATTCCTC 
CCATTTCCCT TTGAGCAGTC AGGGGGAGCC CCTCAGTCTG AAAACCTGGG TATCCATTCT 
GCCGCTCACC ACACAGTTCA GCCTGAAGTC AGCACCCCAG TGCTGATCAC TCCCGCCTCC 
ATTGCACAGT CGGGTGACAA ACATCCCCCA AGCTACACAC TCCCACTGAG CCCTGTCCTC 
AGCCCCACTC TGCCAGCTGA AGCTCCTACC ACACAAGTTC CCCACTTACC TCGGAATAGC 
ACCATGATAC AAGCAGTAAA GACCACACCC AGGCAGAAAT CTCCACTCCT GAACCAGCCA 
GTCCCTGAGC TAAGCCACAG CAGTCTGGTT GCTAGCCAGA GCCCCTTCAG AGCCCCGGTG 
AGCCTAGCAA ACCCAGCTGG CACAGCCCTC CCAAGCGTTG ATCTGCTTCA GAAACTCAGG 
TTGACCCCAC AGCATGACCA AATACAAGCA CAGCCGCTTG GAAAAGGAAC CATGGCGCCC 
AGCTTTTCTT CGGCAGCTGG CCAACTGGCC ACCCCTGAGA GCTTCATAGA GCCTTCTTCT 
AAGACAGCAG CAGCAAGAGC AGCAGTCTCA GCCTCCCTGA GCAACATGGT GCTTGCTCCA 
ACCCTCCAGT CTATGCAGCA AAACCAGGAT CCTGAAGTAT TTTCCCAGCC TAAGGTGTTA 
CCCAGTGCCA TCCCGATTGC AGGCTCTCCA TTGGTTCCTG CAACCACCAC AGCAGTATCT 
TCAGTCCTGC TGTCCCCCAG TGTGTTCCAG CAAACGGTTC CCAGGGCCGC AGACCTAGAG 
AGGAAAGCCA GCTCCCCCTC TCCCCTCACT GTTGGGACAG CAGAGAGTCA GAGAAAGCCT 
TCCATTATCC TCAGCAAGTC TCAGCTCCAG GACACACTGA TACACCTAAT AAAGAATGAC 
TCCAGCTTCC TCAGTACGCT TCATGCAGTT TACTTGCAGG TTCTGACCAA GAACAAAGAC 
AACCACAACC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline.
eLife6(2017 Oct)
Bailey AS,Batista PJ,Gold RS,Chen YG,de Rooij DG,Chang HY,Fuller MT

Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells.
Nature communications711272(2016 Apr)
Kato Y,Katsuki T,Kokubo H,Masuda A,Saga Y

Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development.
EMBO reports17(1)37-46(2016 Jan)
Suzuki A,Niimi Y,Shinmyozu K,Zhou Z,Kiso M,Saga Y

RNA Binding Protein Nanos2 Organizes Post-transcriptional Buffering System to Retain Primitive State of Mouse Spermatogonial Stem Cells.
Developmental cell34(1)96-107(2015 Jul)
Zhou Z,Shirakawa T,Ohbo K,Sada A,Wu Q,Hasegawa K,Saba R,Saga Y

Interaction of NANOS2 and NANOS3 with different components of the CNOT complex may contribute to the functional differences in mouse male germ cells.
Biology open3(12)1207-16(2014 Nov)
Suzuki A,Niimi Y,Saga Y

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Dcp1a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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