Nln cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nln
Entrez Gene ID	75805
Full Name	neurolysin (metallopeptidase M3 family)
Synonyms	4930472G13Rik,C79345
General protein information	Preferred Names neurolysin (metallopeptidase M3 family) Names neurolysin, mitochondrial MEP microsomal endopeptidase mitochondrial oligopeptidase M neurotensin endopeptidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011244701.2	XP_011243003.1	neurolysin, mitochondrial isoform X2
NM_029447.2	NP_083723.1	neurolysin, mitochondrial precursor
XM_011244701.1	XP_011243003.1	neurolysin, mitochondrial isoform X2
XM_011244699.2	XP_011243001.1	neurolysin, mitochondrial isoform X1
XM_011244699.1	XP_011243001.1	neurolysin, mitochondrial isoform X1
XM_011244700.1	XP_011243002.1	neurolysin, mitochondrial isoform X2
XM_030247452.1	XP_030103312.1	neurolysin, mitochondrial isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NLN	XP_001163138.1
Rattus norvegicus (Norway rat)	Nln	NP_446422.2
Magnaporthe oryzae (rice blast fungus)	MGG_04858	XP_003712337.1
Mus musculus (house mouse)	Nln	NP_083723.1
	LOC696884	XP_001087239.1
Kluyveromyces lactis	KLLA0C00572g	XP_452219.1
Neurospora crassa	NCU06618	XP_962360.1
Homo sapiens (human)	NLN	NP_065777.1
Canis lupus familiaris (dog)	NLN	XP_005617505.1
Bos taurus (cattle)	NLN	NP_001029161.2
Saccharomyces cerevisiae (baker's yeast)	PRD1	NP_009874.1
Xenopus tropicalis (tropical clawed frog)	nln	XP_002934236.2
Gallus gallus (chicken)	NLN	XP_424755.2

Gene Ontology
Bibliography

Related articles in PubMed

Hepatic scavenger receptor BI protects against polymicrobial-induced sepsis through promoting LPS clearance in mice.
Guo L, Zheng Z, Ai J, Huang B, Li XA
The Journal of biological chemistry289(21)14666-73(2014 May)

Neurolysin knockout mice generation and initial phenotype characterization.
Cavalcanti DM, Castro LM, Rosa Neto JC, Seelaender M, Neves RX, Oliveira V, Forti FL, Iwai LK, Gozzo FC, Todiras M, Schadock I, Barros CC, Bader M, Ferro ES
The Journal of biological chemistry289(22)15426-40(2014 May)

Functional up-regulation of endopeptidase neurolysin during post-acute and early recovery phases of experimental stroke in mouse brain.
Rashid M, Wangler NJ, Yang L, Shah K, Arumugam TV, Abbruscato TJ, Karamyan VT
Journal of neurochemistry129(1)179-89(2014 Apr)

Distribution of non-AT1, non-AT2 binding of 125I-sarcosine1, isoleucine8 angiotensin II in neurolysin knockout mouse brains.
Speth RC, Carrera EJ, Bret?n C, Linares A, Gonzalez-Reiley L, Swindle JD, Santos KL, Schadock I, Bader M, Karamyan VT
PloS one9(8)e105762(2014)

Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.
Nakahara M, Tateyama H, Araki M, Nakagata N, Yamamura K, Araki K
Mammalian genome : official journal of the International Mammalian Genome Society24(5-6)228-39(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest the exciting new possibility that Nln is a key enzyme for energy metabolism and could be a novel therapeutic target to improve glucose uptake and insulin sensitivity.
Title: Neurolysin knockout mice generation and initial phenotype characterization.

Neurolysin plays a role in modulating the brain's response to stroke and recovery after stroke.
Title: Functional up-regulation of endopeptidase neurolysin during post-acute and early recovery phases of experimental stroke in mouse brain.

These results confirm the identity of neurolysin as a novel Ang II binding site, suggesting that neurolysin may play a significant role in opposing the pathophysiological actions of the brain RAS and influencing brain morphology.
Title: Distribution of non-AT1, non-AT2 binding of 125I-sarcosine1, isoleucine8 angiotensin II in neurolysin knockout mouse brains.

novel angiotensin binding site
Title: Identification of membrane-bound variant of metalloendopeptidase neurolysin (EC 3.4.24.16) as the non-angiotensin type 1 (non-AT1), non-AT2 angiotensin binding site.

Melanoma cells secrete neurolysin which may have an important role in tumor proliferation/angiogenesis in vitro and in vivo.
Title: Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growth.

The following Nln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu71409	XM_011244700.1 Latest version!	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu22085	NM_029447.2 Latest version!	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu71408	XM_011244699.2 Latest version!	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu71409	XM_011244701.2 Latest version!	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu86297	XM_030247452.1 Latest version!	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu71409	XM_011244701.1	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu71408	XM_011244699.1	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nln ( XM_011244699.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243001.1 Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu71408
Clone ID Related Accession (Same CDS sequence)	XM_011244699.2 , XM_011244699.1
Accession Version	XM_011244699.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurolysin, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACCC TGTGCCTTTC GGCTCTGCGA GGCCTCCACA GAGCTGGTGG TTCCAGGATT 
CGGCTCAGAA TGACGTTGGG GAGGGAGGCG GCATCTCCTC TTCAGGCCAT GTCTTCCTAC 
ACCGCCGCAG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAGGACGAGG 
ACAGAGGAGC TCATAGCACA GACCAAGCAG GTGTATGATA CCGTGGGGAC AATCAACCTG 
GAGGATGTAA CTTACGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GAAGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCGT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCGGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTATTTC AGAGAATTGT TCATTTACAA GAAACGTGCG ATTTGGAGAA GATAAAGCCT 
GAGGCCAGGC GATACTTGGA AAAATCAATT AAAATGGGGA AAAGGAATGG ACTCCATCTT 
CCAGAACACG TAAAAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AAAACCTCAA TGAGGATGAC ACGTCCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CCGACGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCCCA CTATTTTCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAG AGGTAAGAAC ACTTACTGCT 
CTGGCTGACG ACCCGGATTT GGTTCCCAGC ACCACATGGT GTCCTGCAGC CATCAGTAAA 
TCCAGTACCA GGGGATCTGA TGACCCATTT GGACCCCTCA AGCTCCAGGA AAACACCATA 
ATTCTGCAAC AGCTCCTGCC GCTGCGAGCA CAAGTGGCCA AACTGCTCGG CTACAACACC 
CATGCTGACT TTGTCCTTGA ACTGAACACT GCAAAGAGCA CAAGCCATGT GGCAACCTTT 
CTAGATGATT TAAGCCAGAA ATTAAAACCC TTGGGTGAGG CTGAACGGGA GTTTATTTTG 
AGCTTGAAGA AGAAGGAATG CGAAGAGCGA GGTTTTGCAT ACGACGGGAA GATCAACGCA 
TGGGACCTCC ATTACTACAT GACCCAGACA GAGGAGCTCA AGTACTCTGT GGACCAGGAG 
TCCCTCAAGG AGTACTTTCC CATTGAGGTG GTCACTGAAG GCCTGCTAAG CATCTACCAG 
GAGCTGTTGG GACTTTCATT TGAACAAGTG GCTGATGCCC ATGTTTGGAA TAAGAGTGTT 
TCACTTTACA CCGTGAAGGA TAAAGCTACT GGAGAAGTGC TGGGGCAGTT CTACCTGGAC 
CTCTATCCAA GGGAAGGAAA ATACAACCAT GCGGCCTGCT TTGGTCTCCA GCCAGGCTGC 
CTTCTCCCTG ATGGCAGTCG GATGATGTCT GTGGCTGCCC TGGTGGTCAA CTTCTCTCAG 
CCCATAGCAG GCCGGCCCTC TCTCCTGAGA CACGACGAAG TGCGAACTTA CTTCCATGAG 
TTTGGTCACG TCATGCATCA GATCTGTGCA CAGACTGACT TTGCACGATT TAGTGGAACA 
AATGTGGAAA CTGACTTTGT AGAGGTGCCA TCACAAATGC TTGAAAACTG GGTGTGGGAC 
ATTGACTCCC TGCGAAAACT GTCAAAACAT TATAGAGACG GACACCCTAT CACAGACGAG 
CTGCTGGAGA AGCTTGTGGC TTCGAGACTG GTCAACACAG GTCTTCTGAC GCTTCGCCAA 
ATTGTTTTGA GCAAAGTTGA CCAGTCCCTC CATACCAATG CGTCGCTGGA TGCCGCGAGC 
GAATACGCTA AATACTGCAC AGAAATTTTG GGAGTTGCAG CTACGCCAGG CACAAATATG 
CCAGCTACCT TTGGACATTT GGCAGGAGGG TATGACGGCC AGTATTATGG ATATCTTTGG 
AGTGAAGTCT TTTCCATGGA CATGTTTCAC AGCTGTTTCA GAAAAGAAGG GATTATGAAT 
CCAGAGGTTG GAATGAAATA CAGAAACCTA ATCCTGAAGC CTGGGGGGTC CCTGGACGGC 
ATGGACATGC TCCAGAATTT CTTGCAACGT GAGCCAAACC AGAAAGCATT CCTGATGAGT 
CGAGGCCTGA ATGCTTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243001.1
CDS	205..2445
Translation	MITLCLSALRGLHRAGGSRIRLRMTLGREAASPLQAMSSYTAAGRNVLRWDLSPEQIRTRTEELIAQTKQVYDTVGTINLEDVTYENCLQVLADIEVKYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLPEHVKNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKEVRTLTALADDPDLVPSTTWCPAAISKSSTRGSDDPFGPLKLQENTIILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSHVATFLDDLSQKLKPLGEAEREFILSLKKKECEERGFAYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVADAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPIAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDIDSLRKLSKHYRDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNASLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFRKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNAS

Target ORF information:

RefSeq Version	XM_011244699.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244699.1

ORF Insert Sequence:

ATGATCACCC TGTGCCTTTC GGCTCTGCGA GGCCTCCACA GAGCTGGTGG TTCCAGGATT 
CGGCTCAGAA TGACGTTGGG GAGGGAGGCG GCATCTCCTC TTCAGGCCAT GTCTTCCTAC 
ACCGCCGCAG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAGGACGAGG 
ACAGAGGAGC TCATAGCACA GACCAAGCAG GTGTATGATA CCGTGGGGAC AATCAACCTG 
GAGGATGTAA CTTACGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GAAGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCGT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCGGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTATTTC AGAGAATTGT TCATTTACAA GAAACGTGCG ATTTGGAGAA GATAAAGCCT 
GAGGCCAGGC GATACTTGGA AAAATCAATT AAAATGGGGA AAAGGAATGG ACTCCATCTT 
CCAGAACACG TAAAAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AAAACCTCAA TGAGGATGAC ACGTCCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CCGACGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCCCA CTATTTTCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAG AGGTAAGAAC ACTTACTGCT 
CTGGCTGACG ACCCGGATTT GGTTCCCAGC ACCACATGGT GTCCTGCAGC CATCAGTAAA 
TCCAGTACCA GGGGATCTGA TGACCCATTT GGACCCCTCA AGCTCCAGGA AAACACCATA 
ATTCTGCAAC AGCTCCTGCC GCTGCGAGCA CAAGTGGCCA AACTGCTCGG CTACAACACC 
CATGCTGACT TTGTCCTTGA ACTGAACACT GCAAAGAGCA CAAGCCATGT GGCAACCTTT 
CTAGATGATT TAAGCCAGAA ATTAAAACCC TTGGGTGAGG CTGAACGGGA GTTTATTTTG 
AGCTTGAAGA AGAAGGAATG CGAAGAGCGA GGTTTTGCAT ACGACGGGAA GATCAACGCA 
TGGGACCTCC ATTACTACAT GACCCAGACA GAGGAGCTCA AGTACTCTGT GGACCAGGAG 
TCCCTCAAGG AGTACTTTCC CATTGAGGTG GTCACTGAAG GCCTGCTAAG CATCTACCAG 
GAGCTGTTGG GACTTTCATT TGAACAAGTG GCTGATGCCC ATGTTTGGAA TAAGAGTGTT 
TCACTTTACA CCGTGAAGGA TAAAGCTACT GGAGAAGTGC TGGGGCAGTT CTACCTGGAC 
CTCTATCCAA GGGAAGGAAA ATACAACCAT GCGGCCTGCT TTGGTCTCCA GCCAGGCTGC 
CTTCTCCCTG ATGGCAGTCG GATGATGTCT GTGGCTGCCC TGGTGGTCAA CTTCTCTCAG 
CCCATAGCAG GCCGGCCCTC TCTCCTGAGA CACGACGAAG TGCGAACTTA CTTCCATGAG 
TTTGGTCACG TCATGCATCA GATCTGTGCA CAGACTGACT TTGCACGATT TAGTGGAACA 
AATGTGGAAA CTGACTTTGT AGAGGTGCCA TCACAAATGC TTGAAAACTG GGTGTGGGAC 
ATTGACTCCC TGCGAAAACT GTCAAAACAT TATAGAGACG GACACCCTAT CACAGACGAG 
CTGCTGGAGA AGCTTGTGGC TTCGAGACTG GTCAACACAG GTCTTCTGAC GCTTCGCCAA 
ATTGTTTTGA GCAAAGTTGA CCAGTCCCTC CATACCAATG CGTCGCTGGA TGCCGCGAGC 
GAATACGCTA AATACTGCAC AGAAATTTTG GGAGTTGCAG CTACGCCAGG CACAAATATG 
CCAGCTACCT TTGGACATTT GGCAGGAGGG TATGACGGCC AGTATTATGG ATATCTTTGG 
AGTGAAGTCT TTTCCATGGA CATGTTTCAC AGCTGTTTCA GAAAAGAAGG GATTATGAAT 
CCAGAGGTTG GAATGAAATA CAGAAACCTA ATCCTGAAGC CTGGGGGGTC CCTGGACGGC 
ATGGACATGC TCCAGAATTT CTTGCAACGT GAGCCAAACC AGAAAGCATT CCTGATGAGT 
CGAGGCCTGA ATGCTTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nln ( XM_011244701.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243003.1 Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu71409
Clone ID Related Accession (Same CDS sequence)	XM_011244701.2 , XM_011244701.1 , XM_011244700.1
Accession Version	XM_011244701.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurolysin, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTGG GGAGGGAGGC GGCATCTCCT CTTCAGGCCA TGTCTTCCTA CACCGCCGCA 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAGGACGAG GACAGAGGAG 
CTCATAGCAC AGACCAAGCA GGTGTATGAT ACCGTGGGGA CAATCAACCT GGAGGATGTA 
ACTTACGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGAAGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCG TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCGGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTATTT 
CAGAGAATTG TTCATTTACA AGAAACGTGC GATTTGGAGA AGATAAAGCC TGAGGCCAGG 
CGATACTTGG AAAAATCAAT TAAAATGGGG AAAAGGAATG GACTCCATCT TCCAGAACAC 
GTAAAAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAAAACCTCA ATGAGGATGA CACGTCCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCCGACGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCCC ACTATTTTCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA GAGGTAAGAA CACTTACTGC TCTGGCTGAC 
GACCCGGATT TGGTTCCCAG CACCACATGG TGTCCTGCAG CCATCAGTAA ATCCAGTACC 
AGGGGATCTG ATGACCCATT TGGACCCCTC AAGCTCCAGG AAAACACCAT AATTCTGCAA 
CAGCTCCTGC CGCTGCGAGC ACAAGTGGCC AAACTGCTCG GCTACAACAC CCATGCTGAC 
TTTGTCCTTG AACTGAACAC TGCAAAGAGC ACAAGCCATG TGGCAACCTT TCTAGATGAT 
TTAAGCCAGA AATTAAAACC CTTGGGTGAG GCTGAACGGG AGTTTATTTT GAGCTTGAAG 
AAGAAGGAAT GCGAAGAGCG AGGTTTTGCA TACGACGGGA AGATCAACGC ATGGGACCTC 
CATTACTACA TGACCCAGAC AGAGGAGCTC AAGTACTCTG TGGACCAGGA GTCCCTCAAG 
GAGTACTTTC CCATTGAGGT GGTCACTGAA GGCCTGCTAA GCATCTACCA GGAGCTGTTG 
GGACTTTCAT TTGAACAAGT GGCTGATGCC CATGTTTGGA ATAAGAGTGT TTCACTTTAC 
ACCGTGAAGG ATAAAGCTAC TGGAGAAGTG CTGGGGCAGT TCTACCTGGA CCTCTATCCA 
AGGGAAGGAA AATACAACCA TGCGGCCTGC TTTGGTCTCC AGCCAGGCTG CCTTCTCCCT 
GATGGCAGTC GGATGATGTC TGTGGCTGCC CTGGTGGTCA ACTTCTCTCA GCCCATAGCA 
GGCCGGCCCT CTCTCCTGAG ACACGACGAA GTGCGAACTT ACTTCCATGA GTTTGGTCAC 
GTCATGCATC AGATCTGTGC ACAGACTGAC TTTGCACGAT TTAGTGGAAC AAATGTGGAA 
ACTGACTTTG TAGAGGTGCC ATCACAAATG CTTGAAAACT GGGTGTGGGA CATTGACTCC 
CTGCGAAAAC TGTCAAAACA TTATAGAGAC GGACACCCTA TCACAGACGA GCTGCTGGAG 
AAGCTTGTGG CTTCGAGACT GGTCAACACA GGTCTTCTGA CGCTTCGCCA AATTGTTTTG 
AGCAAAGTTG ACCAGTCCCT CCATACCAAT GCGTCGCTGG ATGCCGCGAG CGAATACGCT 
AAATACTGCA CAGAAATTTT GGGAGTTGCA GCTACGCCAG GCACAAATAT GCCAGCTACC 
TTTGGACATT TGGCAGGAGG GTATGACGGC CAGTATTATG GATATCTTTG GAGTGAAGTC 
TTTTCCATGG ACATGTTTCA CAGCTGTTTC AGAAAAGAAG GGATTATGAA TCCAGAGGTT 
GGAATGAAAT ACAGAAACCT AATCCTGAAG CCTGGGGGGT CCCTGGACGG CATGGACATG 
CTCCAGAATT TCTTGCAACG TGAGCCAAAC CAGAAAGCAT TCCTGATGAG TCGAGGCCTG 
AATGCTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243003.1
CDS	279..2450
Translation	MTLGREAASPLQAMSSYTAAGRNVLRWDLSPEQIRTRTEELIAQTKQVYDTVGTINLEDVTYENCLQVLADIEVKYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLPEHVKNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKEVRTLTALADDPDLVPSTTWCPAAISKSSTRGSDDPFGPLKLQENTIILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSHVATFLDDLSQKLKPLGEAEREFILSLKKKECEERGFAYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVADAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPIAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDIDSLRKLSKHYRDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNASLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFRKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNAS

Target ORF information:

RefSeq Version	XM_011244701.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244701.1

ORF Insert Sequence:

ATGACGTTGG GGAGGGAGGC GGCATCTCCT CTTCAGGCCA TGTCTTCCTA CACCGCCGCA 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAGGACGAG GACAGAGGAG 
CTCATAGCAC AGACCAAGCA GGTGTATGAT ACCGTGGGGA CAATCAACCT GGAGGATGTA 
ACTTACGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGAAGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCG TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCGGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTATTT 
CAGAGAATTG TTCATTTACA AGAAACGTGC GATTTGGAGA AGATAAAGCC TGAGGCCAGG 
CGATACTTGG AAAAATCAAT TAAAATGGGG AAAAGGAATG GACTCCATCT TCCAGAACAC 
GTAAAAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAAAACCTCA ATGAGGATGA CACGTCCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCCGACGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCCC ACTATTTTCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA GAGGTAAGAA CACTTACTGC TCTGGCTGAC 
GACCCGGATT TGGTTCCCAG CACCACATGG TGTCCTGCAG CCATCAGTAA ATCCAGTACC 
AGGGGATCTG ATGACCCATT TGGACCCCTC AAGCTCCAGG AAAACACCAT AATTCTGCAA 
CAGCTCCTGC CGCTGCGAGC ACAAGTGGCC AAACTGCTCG GCTACAACAC CCATGCTGAC 
TTTGTCCTTG AACTGAACAC TGCAAAGAGC ACAAGCCATG TGGCAACCTT TCTAGATGAT 
TTAAGCCAGA AATTAAAACC CTTGGGTGAG GCTGAACGGG AGTTTATTTT GAGCTTGAAG 
AAGAAGGAAT GCGAAGAGCG AGGTTTTGCA TACGACGGGA AGATCAACGC ATGGGACCTC 
CATTACTACA TGACCCAGAC AGAGGAGCTC AAGTACTCTG TGGACCAGGA GTCCCTCAAG 
GAGTACTTTC CCATTGAGGT GGTCACTGAA GGCCTGCTAA GCATCTACCA GGAGCTGTTG 
GGACTTTCAT TTGAACAAGT GGCTGATGCC CATGTTTGGA ATAAGAGTGT TTCACTTTAC 
ACCGTGAAGG ATAAAGCTAC TGGAGAAGTG CTGGGGCAGT TCTACCTGGA CCTCTATCCA 
AGGGAAGGAA AATACAACCA TGCGGCCTGC TTTGGTCTCC AGCCAGGCTG CCTTCTCCCT 
GATGGCAGTC GGATGATGTC TGTGGCTGCC CTGGTGGTCA ACTTCTCTCA GCCCATAGCA 
GGCCGGCCCT CTCTCCTGAG ACACGACGAA GTGCGAACTT ACTTCCATGA GTTTGGTCAC 
GTCATGCATC AGATCTGTGC ACAGACTGAC TTTGCACGAT TTAGTGGAAC AAATGTGGAA 
ACTGACTTTG TAGAGGTGCC ATCACAAATG CTTGAAAACT GGGTGTGGGA CATTGACTCC 
CTGCGAAAAC TGTCAAAACA TTATAGAGAC GGACACCCTA TCACAGACGA GCTGCTGGAG 
AAGCTTGTGG CTTCGAGACT GGTCAACACA GGTCTTCTGA CGCTTCGCCA AATTGTTTTG 
AGCAAAGTTG ACCAGTCCCT CCATACCAAT GCGTCGCTGG ATGCCGCGAG CGAATACGCT 
AAATACTGCA CAGAAATTTT GGGAGTTGCA GCTACGCCAG GCACAAATAT GCCAGCTACC 
TTTGGACATT TGGCAGGAGG GTATGACGGC CAGTATTATG GATATCTTTG GAGTGAAGTC 
TTTTCCATGG ACATGTTTCA CAGCTGTTTC AGAAAAGAAG GGATTATGAA TCCAGAGGTT 
GGAATGAAAT ACAGAAACCT AATCCTGAAG CCTGGGGGGT CCCTGGACGG CATGGACATG 
CTCCAGAATT TCTTGCAACG TGAGCCAAAC CAGAAAGCAT TCCTGATGAG TCGAGGCCTG 
AATGCTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nln ( XM_011244700.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243002.1 Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X2, mRNA.

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CloneID	OMu71409
Clone ID Related Accession (Same CDS sequence)	XM_011244701.2 , XM_011244701.1 , XM_011244700.1
Accession Version	XM_011244700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurolysin, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTGG GGAGGGAGGC GGCATCTCCT CTTCAGGCCA TGTCTTCCTA CACCGCCGCA 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAGGACGAG GACAGAGGAG 
CTCATAGCAC AGACCAAGCA GGTGTATGAT ACCGTGGGGA CAATCAACCT GGAGGATGTA 
ACTTACGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGAAGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCG TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCGGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTATTT 
CAGAGAATTG TTCATTTACA AGAAACGTGC GATTTGGAGA AGATAAAGCC TGAGGCCAGG 
CGATACTTGG AAAAATCAAT TAAAATGGGG AAAAGGAATG GACTCCATCT TCCAGAACAC 
GTAAAAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAAAACCTCA ATGAGGATGA CACGTCCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCCGACGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCCC ACTATTTTCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA GAGGTAAGAA CACTTACTGC TCTGGCTGAC 
GACCCGGATT TGGTTCCCAG CACCACATGG TGTCCTGCAG CCATCAGTAA ATCCAGTACC 
AGGGGATCTG ATGACCCATT TGGACCCCTC AAGCTCCAGG AAAACACCAT AATTCTGCAA 
CAGCTCCTGC CGCTGCGAGC ACAAGTGGCC AAACTGCTCG GCTACAACAC CCATGCTGAC 
TTTGTCCTTG AACTGAACAC TGCAAAGAGC ACAAGCCATG TGGCAACCTT TCTAGATGAT 
TTAAGCCAGA AATTAAAACC CTTGGGTGAG GCTGAACGGG AGTTTATTTT GAGCTTGAAG 
AAGAAGGAAT GCGAAGAGCG AGGTTTTGCA TACGACGGGA AGATCAACGC ATGGGACCTC 
CATTACTACA TGACCCAGAC AGAGGAGCTC AAGTACTCTG TGGACCAGGA GTCCCTCAAG 
GAGTACTTTC CCATTGAGGT GGTCACTGAA GGCCTGCTAA GCATCTACCA GGAGCTGTTG 
GGACTTTCAT TTGAACAAGT GGCTGATGCC CATGTTTGGA ATAAGAGTGT TTCACTTTAC 
ACCGTGAAGG ATAAAGCTAC TGGAGAAGTG CTGGGGCAGT TCTACCTGGA CCTCTATCCA 
AGGGAAGGAA AATACAACCA TGCGGCCTGC TTTGGTCTCC AGCCAGGCTG CCTTCTCCCT 
GATGGCAGTC GGATGATGTC TGTGGCTGCC CTGGTGGTCA ACTTCTCTCA GCCCATAGCA 
GGCCGGCCCT CTCTCCTGAG ACACGACGAA GTGCGAACTT ACTTCCATGA GTTTGGTCAC 
GTCATGCATC AGATCTGTGC ACAGACTGAC TTTGCACGAT TTAGTGGAAC AAATGTGGAA 
ACTGACTTTG TAGAGGTGCC ATCACAAATG CTTGAAAACT GGGTGTGGGA CATTGACTCC 
CTGCGAAAAC TGTCAAAACA TTATAGAGAC GGACACCCTA TCACAGACGA GCTGCTGGAG 
AAGCTTGTGG CTTCGAGACT GGTCAACACA GGTCTTCTGA CGCTTCGCCA AATTGTTTTG 
AGCAAAGTTG ACCAGTCCCT CCATACCAAT GCGTCGCTGG ATGCCGCGAG CGAATACGCT 
AAATACTGCA CAGAAATTTT GGGAGTTGCA GCTACGCCAG GCACAAATAT GCCAGCTACC 
TTTGGACATT TGGCAGGAGG GTATGACGGC CAGTATTATG GATATCTTTG GAGTGAAGTC 
TTTTCCATGG ACATGTTTCA CAGCTGTTTC AGAAAAGAAG GGATTATGAA TCCAGAGGTT 
GGAATGAAAT ACAGAAACCT AATCCTGAAG CCTGGGGGGT CCCTGGACGG CATGGACATG 
CTCCAGAATT TCTTGCAACG TGAGCCAAAC CAGAAAGCAT TCCTGATGAG TCGAGGCCTG 
AATGCTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243002.1
CDS	417..2588
Translation	MTLGREAASPLQAMSSYTAAGRNVLRWDLSPEQIRTRTEELIAQTKQVYDTVGTINLEDVTYENCLQVLADIEVKYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLPEHVKNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKEVRTLTALADDPDLVPSTTWCPAAISKSSTRGSDDPFGPLKLQENTIILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSHVATFLDDLSQKLKPLGEAEREFILSLKKKECEERGFAYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVADAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPIAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDIDSLRKLSKHYRDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNASLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFRKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNAS

Target ORF information:

RefSeq Version	XM_011244700.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244700.1

ORF Insert Sequence:

ATGACGTTGG GGAGGGAGGC GGCATCTCCT CTTCAGGCCA TGTCTTCCTA CACCGCCGCA 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAGGACGAG GACAGAGGAG 
CTCATAGCAC AGACCAAGCA GGTGTATGAT ACCGTGGGGA CAATCAACCT GGAGGATGTA 
ACTTACGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGAAGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCG TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCGGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTATTT 
CAGAGAATTG TTCATTTACA AGAAACGTGC GATTTGGAGA AGATAAAGCC TGAGGCCAGG 
CGATACTTGG AAAAATCAAT TAAAATGGGG AAAAGGAATG GACTCCATCT TCCAGAACAC 
GTAAAAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAAAACCTCA ATGAGGATGA CACGTCCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCCGACGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCCC ACTATTTTCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA GAGGTAAGAA CACTTACTGC TCTGGCTGAC 
GACCCGGATT TGGTTCCCAG CACCACATGG TGTCCTGCAG CCATCAGTAA ATCCAGTACC 
AGGGGATCTG ATGACCCATT TGGACCCCTC AAGCTCCAGG AAAACACCAT AATTCTGCAA 
CAGCTCCTGC CGCTGCGAGC ACAAGTGGCC AAACTGCTCG GCTACAACAC CCATGCTGAC 
TTTGTCCTTG AACTGAACAC TGCAAAGAGC ACAAGCCATG TGGCAACCTT TCTAGATGAT 
TTAAGCCAGA AATTAAAACC CTTGGGTGAG GCTGAACGGG AGTTTATTTT GAGCTTGAAG 
AAGAAGGAAT GCGAAGAGCG AGGTTTTGCA TACGACGGGA AGATCAACGC ATGGGACCTC 
CATTACTACA TGACCCAGAC AGAGGAGCTC AAGTACTCTG TGGACCAGGA GTCCCTCAAG 
GAGTACTTTC CCATTGAGGT GGTCACTGAA GGCCTGCTAA GCATCTACCA GGAGCTGTTG 
GGACTTTCAT TTGAACAAGT GGCTGATGCC CATGTTTGGA ATAAGAGTGT TTCACTTTAC 
ACCGTGAAGG ATAAAGCTAC TGGAGAAGTG CTGGGGCAGT TCTACCTGGA CCTCTATCCA 
AGGGAAGGAA AATACAACCA TGCGGCCTGC TTTGGTCTCC AGCCAGGCTG CCTTCTCCCT 
GATGGCAGTC GGATGATGTC TGTGGCTGCC CTGGTGGTCA ACTTCTCTCA GCCCATAGCA 
GGCCGGCCCT CTCTCCTGAG ACACGACGAA GTGCGAACTT ACTTCCATGA GTTTGGTCAC 
GTCATGCATC AGATCTGTGC ACAGACTGAC TTTGCACGAT TTAGTGGAAC AAATGTGGAA 
ACTGACTTTG TAGAGGTGCC ATCACAAATG CTTGAAAACT GGGTGTGGGA CATTGACTCC 
CTGCGAAAAC TGTCAAAACA TTATAGAGAC GGACACCCTA TCACAGACGA GCTGCTGGAG 
AAGCTTGTGG CTTCGAGACT GGTCAACACA GGTCTTCTGA CGCTTCGCCA AATTGTTTTG 
AGCAAAGTTG ACCAGTCCCT CCATACCAAT GCGTCGCTGG ATGCCGCGAG CGAATACGCT 
AAATACTGCA CAGAAATTTT GGGAGTTGCA GCTACGCCAG GCACAAATAT GCCAGCTACC 
TTTGGACATT TGGCAGGAGG GTATGACGGC CAGTATTATG GATATCTTTG GAGTGAAGTC 
TTTTCCATGG ACATGTTTCA CAGCTGTTTC AGAAAAGAAG GGATTATGAA TCCAGAGGTT 
GGAATGAAAT ACAGAAACCT AATCCTGAAG CCTGGGGGGT CCCTGGACGG CATGGACATG 
CTCCAGAATT TCTTGCAACG TGAGCCAAAC CAGAAAGCAT TCCTGATGAG TCGAGGCCTG 
AATGCTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nln ( NM_029447.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083723.1 Mus musculus neurolysin (metallopeptidase M3 family) (Nln), mRNA.

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CloneID	OMu22085
Clone ID Related Accession (Same CDS sequence)	NM_029447.2
Accession Version	NM_029447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	neurolysin, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK079090.1. On Jun 3, 2007 this sequence version replaced NM_029447.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK079090.1, SRR1660817.188455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGATCACCC TGTGCCTTTC GGCTCTGCGA GGCCTCCACA GAGCTGGTGG TTCCAGGATT 
CGGCTCAGAA TGACGTTGGG GAGGGAGGCG GCATCTCCTC TTCAGGCCAT GTCTTCCTAC 
ACCGCCGCAG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAGGACGAGG 
ACAGAGGAGC TCATAGCACA GACCAAGCAG GTGTATGATA CCGTGGGGAC AATCAACCTG 
GAGGATGTAA CTTACGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GAAGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCGT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCGGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTATTTC AGAGAATTGT TCATTTACAA GAAACGTGCG ATTTGGAGAA GATAAAGCCT 
GAGGCCAGGC GATACTTGGA AAAATCAATT AAAATGGGGA AAAGGAATGG ACTCCATCTT 
CCAGAACACG TAAAAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AAAACCTCAA TGAGGATGAC ACGTCCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CCGACGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCCCA CTATTTTCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAG AGGAAAACAC CATAATTCTG 
CAACAGCTCC TGCCGCTGCG AGCACAAGTG GCCAAACTGC TCGGCTACAA CACCCATGCT 
GACTTTGTCC TTGAACTGAA CACTGCAAAG AGCACAAGCC ATGTGGCAAC CTTTCTAGAT 
GATTTAAGCC AGAAATTAAA ACCCTTGGGT GAGGCTGAAC GGGAGTTTAT TTTGAGCTTG 
AAGAAGAAGG AATGCGAAGA GCGAGGTTTT GCATACGACG GGAAGATCAA CGCATGGGAC 
CTCCATTACT ACATGACCCA GACAGAGGAG CTCAAGTACT CTGTGGACCA GGAGTCCCTC 
AAGGAGTACT TTCCCATTGA GGTGGTCACT GAAGGCCTGC TAAGCATCTA CCAGGAGCTG 
TTGGGACTTT CATTTGAACA AGTGGCTGAT GCCCATGTTT GGAATAAGAG TGTTTCACTT 
TACACCGTGA AGGATAAAGC TACTGGAGAA GTGCTGGGGC AGTTCTACCT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA CCATGCGGCC TGCTTTGGTC TCCAGCCAGG CTGCCTTCTC 
CCTGATGGCA GTCGGATGAT GTCTGTGGCT GCCCTGGTGG TCAACTTCTC TCAGCCCATA 
GCAGGCCGGC CCTCTCTCCT GAGACACGAC GAAGTGCGAA CTTACTTCCA TGAGTTTGGT 
CACGTCATGC ATCAGATCTG TGCACAGACT GACTTTGCAC GATTTAGTGG AACAAATGTG 
GAAACTGACT TTGTAGAGGT GCCATCACAA ATGCTTGAAA ACTGGGTGTG GGACATTGAC 
TCCCTGCGAA AACTGTCAAA ACATTATAGA GACGGACACC CTATCACAGA CGAGCTGCTG 
GAGAAGCTTG TGGCTTCGAG ACTGGTCAAC ACAGGTCTTC TGACGCTTCG CCAAATTGTT 
TTGAGCAAAG TTGACCAGTC CCTCCATACC AATGCGTCGC TGGATGCCGC GAGCGAATAC 
GCTAAATACT GCACAGAAAT TTTGGGAGTT GCAGCTACGC CAGGCACAAA TATGCCAGCT 
ACCTTTGGAC ATTTGGCAGG AGGGTATGAC GGCCAGTATT ATGGATATCT TTGGAGTGAA 
GTCTTTTCCA TGGACATGTT TCACAGCTGT TTCAGAAAAG AAGGGATTAT GAATCCAGAG 
GTTGGAATGA AATACAGAAA CCTAATCCTG AAGCCTGGGG GGTCCCTGGA CGGCATGGAC 
ATGCTCCAGA ATTTCTTGCA ACGTGAGCCA AACCAGAAAG CATTCCTGAT GAGTCGAGGC 
CTGAATGCTT CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083723.1
CDS	159..2273
Translation	MITLCLSALRGLHRAGGSRIRLRMTLGREAASPLQAMSSYTAAGRNVLRWDLSPEQIRTRTEELIAQTKQVYDTVGTINLEDVTYENCLQVLADIEVKYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLPEHVKNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKEENTIILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSHVATFLDDLSQKLKPLGEAEREFILSLKKKECEERGFAYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVADAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPIAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDIDSLRKLSKHYRDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNASLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFRKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNAS

Target ORF information:

RefSeq Version	NM_029447.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029447.2

ORF Insert Sequence:

ATGATCACCC TGTGCCTTTC GGCTCTGCGA GGCCTCCACA GAGCTGGTGG TTCCAGGATT 
CGGCTCAGAA TGACGTTGGG GAGGGAGGCG GCATCTCCTC TTCAGGCCAT GTCTTCCTAC 
ACCGCCGCAG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAGGACGAGG 
ACAGAGGAGC TCATAGCACA GACCAAGCAG GTGTATGATA CCGTGGGGAC AATCAACCTG 
GAGGATGTAA CTTACGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GAAGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCGT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCGGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTATTTC AGAGAATTGT TCATTTACAA GAAACGTGCG ATTTGGAGAA GATAAAGCCT 
GAGGCCAGGC GATACTTGGA AAAATCAATT AAAATGGGGA AAAGGAATGG ACTCCATCTT 
CCAGAACACG TAAAAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AAAACCTCAA TGAGGATGAC ACGTCCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CCGACGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCCCA CTATTTTCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAG AGGAAAACAC CATAATTCTG 
CAACAGCTCC TGCCGCTGCG AGCACAAGTG GCCAAACTGC TCGGCTACAA CACCCATGCT 
GACTTTGTCC TTGAACTGAA CACTGCAAAG AGCACAAGCC ATGTGGCAAC CTTTCTAGAT 
GATTTAAGCC AGAAATTAAA ACCCTTGGGT GAGGCTGAAC GGGAGTTTAT TTTGAGCTTG 
AAGAAGAAGG AATGCGAAGA GCGAGGTTTT GCATACGACG GGAAGATCAA CGCATGGGAC 
CTCCATTACT ACATGACCCA GACAGAGGAG CTCAAGTACT CTGTGGACCA GGAGTCCCTC 
AAGGAGTACT TTCCCATTGA GGTGGTCACT GAAGGCCTGC TAAGCATCTA CCAGGAGCTG 
TTGGGACTTT CATTTGAACA AGTGGCTGAT GCCCATGTTT GGAATAAGAG TGTTTCACTT 
TACACCGTGA AGGATAAAGC TACTGGAGAA GTGCTGGGGC AGTTCTACCT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA CCATGCGGCC TGCTTTGGTC TCCAGCCAGG CTGCCTTCTC 
CCTGATGGCA GTCGGATGAT GTCTGTGGCT GCCCTGGTGG TCAACTTCTC TCAGCCCATA 
GCAGGCCGGC CCTCTCTCCT GAGACACGAC GAAGTGCGAA CTTACTTCCA TGAGTTTGGT 
CACGTCATGC ATCAGATCTG TGCACAGACT GACTTTGCAC GATTTAGTGG AACAAATGTG 
GAAACTGACT TTGTAGAGGT GCCATCACAA ATGCTTGAAA ACTGGGTGTG GGACATTGAC 
TCCCTGCGAA AACTGTCAAA ACATTATAGA GACGGACACC CTATCACAGA CGAGCTGCTG 
GAGAAGCTTG TGGCTTCGAG ACTGGTCAAC ACAGGTCTTC TGACGCTTCG CCAAATTGTT 
TTGAGCAAAG TTGACCAGTC CCTCCATACC AATGCGTCGC TGGATGCCGC GAGCGAATAC 
GCTAAATACT GCACAGAAAT TTTGGGAGTT GCAGCTACGC CAGGCACAAA TATGCCAGCT 
ACCTTTGGAC ATTTGGCAGG AGGGTATGAC GGCCAGTATT ATGGATATCT TTGGAGTGAA 
GTCTTTTCCA TGGACATGTT TCACAGCTGT TTCAGAAAAG AAGGGATTAT GAATCCAGAG 
GTTGGAATGA AATACAGAAA CCTAATCCTG AAGCCTGGGG GGTCCCTGGA CGGCATGGAC 
ATGCTCCAGA ATTTCTTGCA ACGTGAGCCA AACCAGAAAG CATTCCTGAT GAGTCGAGGC 
CTGAATGCTT CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nln ( XM_011244699.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243001.1 Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu71408
Clone ID Related Accession (Same CDS sequence)	XM_011244699.2 , XM_011244699.1
Accession Version	XM_011244699.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurolysin, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACCC TGTGCCTTTC GGCTCTGCGA GGCCTCCACA GAGCTGGTGG TTCCAGGATT 
CGGCTCAGAA TGACGTTGGG GAGGGAGGCG GCATCTCCTC TTCAGGCCAT GTCTTCCTAC 
ACCGCCGCAG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAGGACGAGG 
ACAGAGGAGC TCATAGCACA GACCAAGCAG GTGTATGATA CCGTGGGGAC AATCAACCTG 
GAGGATGTAA CTTACGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GAAGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCGT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCGGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTATTTC AGAGAATTGT TCATTTACAA GAAACGTGCG ATTTGGAGAA GATAAAGCCT 
GAGGCCAGGC GATACTTGGA AAAATCAATT AAAATGGGGA AAAGGAATGG ACTCCATCTT 
CCAGAACACG TAAAAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AAAACCTCAA TGAGGATGAC ACGTCCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CCGACGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCCCA CTATTTTCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAG AGGTAAGAAC ACTTACTGCT 
CTGGCTGACG ACCCGGATTT GGTTCCCAGC ACCACATGGT GTCCTGCAGC CATCAGTAAA 
TCCAGTACCA GGGGATCTGA TGACCCATTT GGACCCCTCA AGCTCCAGGA AAACACCATA 
ATTCTGCAAC AGCTCCTGCC GCTGCGAGCA CAAGTGGCCA AACTGCTCGG CTACAACACC 
CATGCTGACT TTGTCCTTGA ACTGAACACT GCAAAGAGCA CAAGCCATGT GGCAACCTTT 
CTAGATGATT TAAGCCAGAA ATTAAAACCC TTGGGTGAGG CTGAACGGGA GTTTATTTTG 
AGCTTGAAGA AGAAGGAATG CGAAGAGCGA GGTTTTGCAT ACGACGGGAA GATCAACGCA 
TGGGACCTCC ATTACTACAT GACCCAGACA GAGGAGCTCA AGTACTCTGT GGACCAGGAG 
TCCCTCAAGG AGTACTTTCC CATTGAGGTG GTCACTGAAG GCCTGCTAAG CATCTACCAG 
GAGCTGTTGG GACTTTCATT TGAACAAGTG GCTGATGCCC ATGTTTGGAA TAAGAGTGTT 
TCACTTTACA CCGTGAAGGA TAAAGCTACT GGAGAAGTGC TGGGGCAGTT CTACCTGGAC 
CTCTATCCAA GGGAAGGAAA ATACAACCAT GCGGCCTGCT TTGGTCTCCA GCCAGGCTGC 
CTTCTCCCTG ATGGCAGTCG GATGATGTCT GTGGCTGCCC TGGTGGTCAA CTTCTCTCAG 
CCCATAGCAG GCCGGCCCTC TCTCCTGAGA CACGACGAAG TGCGAACTTA CTTCCATGAG 
TTTGGTCACG TCATGCATCA GATCTGTGCA CAGACTGACT TTGCACGATT TAGTGGAACA 
AATGTGGAAA CTGACTTTGT AGAGGTGCCA TCACAAATGC TTGAAAACTG GGTGTGGGAC 
ATTGACTCCC TGCGAAAACT GTCAAAACAT TATAGAGACG GACACCCTAT CACAGACGAG 
CTGCTGGAGA AGCTTGTGGC TTCGAGACTG GTCAACACAG GTCTTCTGAC GCTTCGCCAA 
ATTGTTTTGA GCAAAGTTGA CCAGTCCCTC CATACCAATG CGTCGCTGGA TGCCGCGAGC 
GAATACGCTA AATACTGCAC AGAAATTTTG GGAGTTGCAG CTACGCCAGG CACAAATATG 
CCAGCTACCT TTGGACATTT GGCAGGAGGG TATGACGGCC AGTATTATGG ATATCTTTGG 
AGTGAAGTCT TTTCCATGGA CATGTTTCAC AGCTGTTTCA GAAAAGAAGG GATTATGAAT 
CCAGAGGTTG GAATGAAATA CAGAAACCTA ATCCTGAAGC CTGGGGGGTC CCTGGACGGC 
ATGGACATGC TCCAGAATTT CTTGCAACGT GAGCCAAACC AGAAAGCATT CCTGATGAGT 
CGAGGCCTGA ATGCTTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243001.1
CDS	218..2458
Translation	MITLCLSALRGLHRAGGSRIRLRMTLGREAASPLQAMSSYTAAGRNVLRWDLSPEQIRTRTEELIAQTKQVYDTVGTINLEDVTYENCLQVLADIEVKYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLPEHVKNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKEVRTLTALADDPDLVPSTTWCPAAISKSSTRGSDDPFGPLKLQENTIILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSHVATFLDDLSQKLKPLGEAEREFILSLKKKECEERGFAYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVADAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPIAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDIDSLRKLSKHYRDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNASLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFRKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNAS

Target ORF information:

RefSeq Version	XM_011244699.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244699.2

ORF Insert Sequence:

ATGATCACCC TGTGCCTTTC GGCTCTGCGA GGCCTCCACA GAGCTGGTGG TTCCAGGATT 
CGGCTCAGAA TGACGTTGGG GAGGGAGGCG GCATCTCCTC TTCAGGCCAT GTCTTCCTAC 
ACCGCCGCAG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAGGACGAGG 
ACAGAGGAGC TCATAGCACA GACCAAGCAG GTGTATGATA CCGTGGGGAC AATCAACCTG 
GAGGATGTAA CTTACGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GAAGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCGT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCGGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTATTTC AGAGAATTGT TCATTTACAA GAAACGTGCG ATTTGGAGAA GATAAAGCCT 
GAGGCCAGGC GATACTTGGA AAAATCAATT AAAATGGGGA AAAGGAATGG ACTCCATCTT 
CCAGAACACG TAAAAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AAAACCTCAA TGAGGATGAC ACGTCCCTTG TATTTTCCAA GGCTGAACTT 
GGTGCTCTTC CCGACGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCCCA CTATTTTCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAG AGGTAAGAAC ACTTACTGCT 
CTGGCTGACG ACCCGGATTT GGTTCCCAGC ACCACATGGT GTCCTGCAGC CATCAGTAAA 
TCCAGTACCA GGGGATCTGA TGACCCATTT GGACCCCTCA AGCTCCAGGA AAACACCATA 
ATTCTGCAAC AGCTCCTGCC GCTGCGAGCA CAAGTGGCCA AACTGCTCGG CTACAACACC 
CATGCTGACT TTGTCCTTGA ACTGAACACT GCAAAGAGCA CAAGCCATGT GGCAACCTTT 
CTAGATGATT TAAGCCAGAA ATTAAAACCC TTGGGTGAGG CTGAACGGGA GTTTATTTTG 
AGCTTGAAGA AGAAGGAATG CGAAGAGCGA GGTTTTGCAT ACGACGGGAA GATCAACGCA 
TGGGACCTCC ATTACTACAT GACCCAGACA GAGGAGCTCA AGTACTCTGT GGACCAGGAG 
TCCCTCAAGG AGTACTTTCC CATTGAGGTG GTCACTGAAG GCCTGCTAAG CATCTACCAG 
GAGCTGTTGG GACTTTCATT TGAACAAGTG GCTGATGCCC ATGTTTGGAA TAAGAGTGTT 
TCACTTTACA CCGTGAAGGA TAAAGCTACT GGAGAAGTGC TGGGGCAGTT CTACCTGGAC 
CTCTATCCAA GGGAAGGAAA ATACAACCAT GCGGCCTGCT TTGGTCTCCA GCCAGGCTGC 
CTTCTCCCTG ATGGCAGTCG GATGATGTCT GTGGCTGCCC TGGTGGTCAA CTTCTCTCAG 
CCCATAGCAG GCCGGCCCTC TCTCCTGAGA CACGACGAAG TGCGAACTTA CTTCCATGAG 
TTTGGTCACG TCATGCATCA GATCTGTGCA CAGACTGACT TTGCACGATT TAGTGGAACA 
AATGTGGAAA CTGACTTTGT AGAGGTGCCA TCACAAATGC TTGAAAACTG GGTGTGGGAC 
ATTGACTCCC TGCGAAAACT GTCAAAACAT TATAGAGACG GACACCCTAT CACAGACGAG 
CTGCTGGAGA AGCTTGTGGC TTCGAGACTG GTCAACACAG GTCTTCTGAC GCTTCGCCAA 
ATTGTTTTGA GCAAAGTTGA CCAGTCCCTC CATACCAATG CGTCGCTGGA TGCCGCGAGC 
GAATACGCTA AATACTGCAC AGAAATTTTG GGAGTTGCAG CTACGCCAGG CACAAATATG 
CCAGCTACCT TTGGACATTT GGCAGGAGGG TATGACGGCC AGTATTATGG ATATCTTTGG 
AGTGAAGTCT TTTCCATGGA CATGTTTCAC AGCTGTTTCA GAAAAGAAGG GATTATGAAT 
CCAGAGGTTG GAATGAAATA CAGAAACCTA ATCCTGAAGC CTGGGGGGTC CCTGGACGGC 
ATGGACATGC TCCAGAATTT CTTGCAACGT GAGCCAAACC AGAAAGCATT CCTGATGAGT 
CGAGGCCTGA ATGCTTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nln ( XM_011244701.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243003.1 Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X3, mRNA.

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CloneID	OMu71409
Clone ID Related Accession (Same CDS sequence)	XM_011244701.2 , XM_011244701.1 , XM_011244700.1
Accession Version	XM_011244701.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurolysin, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244701.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTGG GGAGGGAGGC GGCATCTCCT CTTCAGGCCA TGTCTTCCTA CACCGCCGCA 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAGGACGAG GACAGAGGAG 
CTCATAGCAC AGACCAAGCA GGTGTATGAT ACCGTGGGGA CAATCAACCT GGAGGATGTA 
ACTTACGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGAAGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCG TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCGGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTATTT 
CAGAGAATTG TTCATTTACA AGAAACGTGC GATTTGGAGA AGATAAAGCC TGAGGCCAGG 
CGATACTTGG AAAAATCAAT TAAAATGGGG AAAAGGAATG GACTCCATCT TCCAGAACAC 
GTAAAAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAAAACCTCA ATGAGGATGA CACGTCCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCCGACGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCCC ACTATTTTCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA GAGGTAAGAA CACTTACTGC TCTGGCTGAC 
GACCCGGATT TGGTTCCCAG CACCACATGG TGTCCTGCAG CCATCAGTAA ATCCAGTACC 
AGGGGATCTG ATGACCCATT TGGACCCCTC AAGCTCCAGG AAAACACCAT AATTCTGCAA 
CAGCTCCTGC CGCTGCGAGC ACAAGTGGCC AAACTGCTCG GCTACAACAC CCATGCTGAC 
TTTGTCCTTG AACTGAACAC TGCAAAGAGC ACAAGCCATG TGGCAACCTT TCTAGATGAT 
TTAAGCCAGA AATTAAAACC CTTGGGTGAG GCTGAACGGG AGTTTATTTT GAGCTTGAAG 
AAGAAGGAAT GCGAAGAGCG AGGTTTTGCA TACGACGGGA AGATCAACGC ATGGGACCTC 
CATTACTACA TGACCCAGAC AGAGGAGCTC AAGTACTCTG TGGACCAGGA GTCCCTCAAG 
GAGTACTTTC CCATTGAGGT GGTCACTGAA GGCCTGCTAA GCATCTACCA GGAGCTGTTG 
GGACTTTCAT TTGAACAAGT GGCTGATGCC CATGTTTGGA ATAAGAGTGT TTCACTTTAC 
ACCGTGAAGG ATAAAGCTAC TGGAGAAGTG CTGGGGCAGT TCTACCTGGA CCTCTATCCA 
AGGGAAGGAA AATACAACCA TGCGGCCTGC TTTGGTCTCC AGCCAGGCTG CCTTCTCCCT 
GATGGCAGTC GGATGATGTC TGTGGCTGCC CTGGTGGTCA ACTTCTCTCA GCCCATAGCA 
GGCCGGCCCT CTCTCCTGAG ACACGACGAA GTGCGAACTT ACTTCCATGA GTTTGGTCAC 
GTCATGCATC AGATCTGTGC ACAGACTGAC TTTGCACGAT TTAGTGGAAC AAATGTGGAA 
ACTGACTTTG TAGAGGTGCC ATCACAAATG CTTGAAAACT GGGTGTGGGA CATTGACTCC 
CTGCGAAAAC TGTCAAAACA TTATAGAGAC GGACACCCTA TCACAGACGA GCTGCTGGAG 
AAGCTTGTGG CTTCGAGACT GGTCAACACA GGTCTTCTGA CGCTTCGCCA AATTGTTTTG 
AGCAAAGTTG ACCAGTCCCT CCATACCAAT GCGTCGCTGG ATGCCGCGAG CGAATACGCT 
AAATACTGCA CAGAAATTTT GGGAGTTGCA GCTACGCCAG GCACAAATAT GCCAGCTACC 
TTTGGACATT TGGCAGGAGG GTATGACGGC CAGTATTATG GATATCTTTG GAGTGAAGTC 
TTTTCCATGG ACATGTTTCA CAGCTGTTTC AGAAAAGAAG GGATTATGAA TCCAGAGGTT 
GGAATGAAAT ACAGAAACCT AATCCTGAAG CCTGGGGGGT CCCTGGACGG CATGGACATG 
CTCCAGAATT TCTTGCAACG TGAGCCAAAC CAGAAAGCAT TCCTGATGAG TCGAGGCCTG 
AATGCTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243003.1
CDS	438..2609
Translation	MTLGREAASPLQAMSSYTAAGRNVLRWDLSPEQIRTRTEELIAQTKQVYDTVGTINLEDVTYENCLQVLADIEVKYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLPEHVKNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKEVRTLTALADDPDLVPSTTWCPAAISKSSTRGSDDPFGPLKLQENTIILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSHVATFLDDLSQKLKPLGEAEREFILSLKKKECEERGFAYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVADAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPIAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDIDSLRKLSKHYRDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNASLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFRKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNAS

Target ORF information:

RefSeq Version	XM_011244701.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244701.2

ORF Insert Sequence:

ATGACGTTGG GGAGGGAGGC GGCATCTCCT CTTCAGGCCA TGTCTTCCTA CACCGCCGCA 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAGGACGAG GACAGAGGAG 
CTCATAGCAC AGACCAAGCA GGTGTATGAT ACCGTGGGGA CAATCAACCT GGAGGATGTA 
ACTTACGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGAAGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCG TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCGGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTATTT 
CAGAGAATTG TTCATTTACA AGAAACGTGC GATTTGGAGA AGATAAAGCC TGAGGCCAGG 
CGATACTTGG AAAAATCAAT TAAAATGGGG AAAAGGAATG GACTCCATCT TCCAGAACAC 
GTAAAAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAAAACCTCA ATGAGGATGA CACGTCCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCCGACGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCCC ACTATTTTCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA GAGGTAAGAA CACTTACTGC TCTGGCTGAC 
GACCCGGATT TGGTTCCCAG CACCACATGG TGTCCTGCAG CCATCAGTAA ATCCAGTACC 
AGGGGATCTG ATGACCCATT TGGACCCCTC AAGCTCCAGG AAAACACCAT AATTCTGCAA 
CAGCTCCTGC CGCTGCGAGC ACAAGTGGCC AAACTGCTCG GCTACAACAC CCATGCTGAC 
TTTGTCCTTG AACTGAACAC TGCAAAGAGC ACAAGCCATG TGGCAACCTT TCTAGATGAT 
TTAAGCCAGA AATTAAAACC CTTGGGTGAG GCTGAACGGG AGTTTATTTT GAGCTTGAAG 
AAGAAGGAAT GCGAAGAGCG AGGTTTTGCA TACGACGGGA AGATCAACGC ATGGGACCTC 
CATTACTACA TGACCCAGAC AGAGGAGCTC AAGTACTCTG TGGACCAGGA GTCCCTCAAG 
GAGTACTTTC CCATTGAGGT GGTCACTGAA GGCCTGCTAA GCATCTACCA GGAGCTGTTG 
GGACTTTCAT TTGAACAAGT GGCTGATGCC CATGTTTGGA ATAAGAGTGT TTCACTTTAC 
ACCGTGAAGG ATAAAGCTAC TGGAGAAGTG CTGGGGCAGT TCTACCTGGA CCTCTATCCA 
AGGGAAGGAA AATACAACCA TGCGGCCTGC TTTGGTCTCC AGCCAGGCTG CCTTCTCCCT 
GATGGCAGTC GGATGATGTC TGTGGCTGCC CTGGTGGTCA ACTTCTCTCA GCCCATAGCA 
GGCCGGCCCT CTCTCCTGAG ACACGACGAA GTGCGAACTT ACTTCCATGA GTTTGGTCAC 
GTCATGCATC AGATCTGTGC ACAGACTGAC TTTGCACGAT TTAGTGGAAC AAATGTGGAA 
ACTGACTTTG TAGAGGTGCC ATCACAAATG CTTGAAAACT GGGTGTGGGA CATTGACTCC 
CTGCGAAAAC TGTCAAAACA TTATAGAGAC GGACACCCTA TCACAGACGA GCTGCTGGAG 
AAGCTTGTGG CTTCGAGACT GGTCAACACA GGTCTTCTGA CGCTTCGCCA AATTGTTTTG 
AGCAAAGTTG ACCAGTCCCT CCATACCAAT GCGTCGCTGG ATGCCGCGAG CGAATACGCT 
AAATACTGCA CAGAAATTTT GGGAGTTGCA GCTACGCCAG GCACAAATAT GCCAGCTACC 
TTTGGACATT TGGCAGGAGG GTATGACGGC CAGTATTATG GATATCTTTG GAGTGAAGTC 
TTTTCCATGG ACATGTTTCA CAGCTGTTTC AGAAAAGAAG GGATTATGAA TCCAGAGGTT 
GGAATGAAAT ACAGAAACCT AATCCTGAAG CCTGGGGGGT CCCTGGACGG CATGGACATG 
CTCCAGAATT TCTTGCAACG TGAGCCAAAC CAGAAAGCAT TCCTGATGAG TCGAGGCCTG 
AATGCTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nln ( XM_030247452.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103312.1 Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu86297
Clone ID Related Accession (Same CDS sequence)	XM_030247452.1
Accession Version	XM_030247452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurolysin, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTTGG GGAGGGAGGC GGCATCTCCT CTTCAGGCCA TGTCTTCCTA CACCGCCGCA 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAGGACGAG GACAGAGGAG 
CTCATAGCAC AGACCAAGCA GGTGTATGAT ACCGTGGGGA CAATCAACCT GGAGGATGTA 
ACTTACGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGAAGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCG TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCGGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTATTT 
CAGAGAATTG TTCATTTACA AGAAACGTGC GATTTGGAGA AGATAAAGCC TGAGGCCAGG 
CGATACTTGG AAAAATCAAT TAAAATGGGG AAAAGGAATG GACTCCATCT TCCAGAACAC 
GTAAAAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAAAACCTCA ATGAGGATGA CACGTCCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCCGACGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCCC ACTATTTTCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA GAGGAAAACA CCATAATTCT GCAACAGCTC 
CTGCCGCTGC GAGCACAAGT GGCCAAACTG CTCGGCTACA ACACCCATGC TGACTTTGTC 
CTTGAACTGA ACACTGCAAA GAGCACAAGC CATGTGGCAA CCTTTCTAGA TGATTTAAGC 
CAGAAATTAA AACCCTTGGG TGAGGCTGAA CGGGAGTTTA TTTTGAGCTT GAAGAAGAAG 
GAATGCGAAG AGCGAGGTTT TGCATACGAC GGGAAGATCA ACGCATGGGA CCTCCATTAC 
TACATGACCC AGACAGAGGA GCTCAAGTAC TCTGTGGACC AGGAGTCCCT CAAGGAGTAC 
TTTCCCATTG AGGTGGTCAC TGAAGGCCTG CTAAGCATCT ACCAGGAGCT GTTGGGACTT 
TCATTTGAAC AAGTGGCTGA TGCCCATGTT TGGAATAAGA GTGTTTCACT TTACACCGTG 
AAGGATAAAG CTACTGGAGA AGTGCTGGGG CAGTTCTACC TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ACCATGCGGC CTGCTTTGGT CTCCAGCCAG GCTGCCTTCT CCCTGATGGC 
AGTCGGATGA TGTCTGTGGC TGCCCTGGTG GTCAACTTCT CTCAGCCCAT AGCAGGCCGG 
CCCTCTCTCC TGAGACACGA CGAAGTGCGA ACTTACTTCC ATGAGTTTGG TCACGTCATG 
CATCAGATCT GTGCACAGAC TGACTTTGCA CGATTTAGTG GAACAAATGT GGAAACTGAC 
TTTGTAGAGG TGCCATCACA AATGCTTGAA AACTGGGTGT GGGACATTGA CTCCCTGCGA 
AAACTGTCAA AACATTATAG AGACGGACAC CCTATCACAG ACGAGCTGCT GGAGAAGCTT 
GTGGCTTCGA GACTGGTCAA CACAGGTCTT CTGACGCTTC GCCAAATTGT TTTGAGCAAA 
GTTGACCAGT CCCTCCATAC CAATGCGTCG CTGGATGCCG CGAGCGAATA CGCTAAATAC 
TGCACAGAAA TTTTGGGAGT TGCAGCTACG CCAGGCACAA ATATGCCAGC TACCTTTGGA 
CATTTGGCAG GAGGGTATGA CGGCCAGTAT TATGGATATC TTTGGAGTGA AGTCTTTTCC 
ATGGACATGT TTCACAGCTG TTTCAGAAAA GAAGGGATTA TGAATCCAGA GGTTGGAATG 
AAATACAGAA ACCTAATCCT GAAGCCTGGG GGGTCCCTGG ACGGCATGGA CATGCTCCAG 
AATTTCTTGC AACGTGAGCC AAACCAGAAA GCATTCCTGA TGAGTCGAGG CCTGAATGCT 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103312.1
CDS	416..2461
Translation	MTLGREAASPLQAMSSYTAAGRNVLRWDLSPEQIRTRTEELIAQTKQVYDTVGTINLEDVTYENCLQVLADIEVKYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLPEHVKNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKEENTIILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSHVATFLDDLSQKLKPLGEAEREFILSLKKKECEERGFAYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVADAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPIAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDIDSLRKLSKHYRDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNASLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFRKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNAS

Target ORF information:

RefSeq Version	XM_030247452.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurolysin (metallopeptidase M3 family) (Nln), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247452.1

ORF Insert Sequence:

ATGACGTTGG GGAGGGAGGC GGCATCTCCT CTTCAGGCCA TGTCTTCCTA CACCGCCGCA 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAGGACGAG GACAGAGGAG 
CTCATAGCAC AGACCAAGCA GGTGTATGAT ACCGTGGGGA CAATCAACCT GGAGGATGTA 
ACTTACGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGAAGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCG TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCGGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTATTT 
CAGAGAATTG TTCATTTACA AGAAACGTGC GATTTGGAGA AGATAAAGCC TGAGGCCAGG 
CGATACTTGG AAAAATCAAT TAAAATGGGG AAAAGGAATG GACTCCATCT TCCAGAACAC 
GTAAAAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAAAACCTCA ATGAGGATGA CACGTCCCTT GTATTTTCCA AGGCTGAACT TGGTGCTCTT 
CCCGACGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCCC ACTATTTTCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA GAGGAAAACA CCATAATTCT GCAACAGCTC 
CTGCCGCTGC GAGCACAAGT GGCCAAACTG CTCGGCTACA ACACCCATGC TGACTTTGTC 
CTTGAACTGA ACACTGCAAA GAGCACAAGC CATGTGGCAA CCTTTCTAGA TGATTTAAGC 
CAGAAATTAA AACCCTTGGG TGAGGCTGAA CGGGAGTTTA TTTTGAGCTT GAAGAAGAAG 
GAATGCGAAG AGCGAGGTTT TGCATACGAC GGGAAGATCA ACGCATGGGA CCTCCATTAC 
TACATGACCC AGACAGAGGA GCTCAAGTAC TCTGTGGACC AGGAGTCCCT CAAGGAGTAC 
TTTCCCATTG AGGTGGTCAC TGAAGGCCTG CTAAGCATCT ACCAGGAGCT GTTGGGACTT 
TCATTTGAAC AAGTGGCTGA TGCCCATGTT TGGAATAAGA GTGTTTCACT TTACACCGTG 
AAGGATAAAG CTACTGGAGA AGTGCTGGGG CAGTTCTACC TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ACCATGCGGC CTGCTTTGGT CTCCAGCCAG GCTGCCTTCT CCCTGATGGC 
AGTCGGATGA TGTCTGTGGC TGCCCTGGTG GTCAACTTCT CTCAGCCCAT AGCAGGCCGG 
CCCTCTCTCC TGAGACACGA CGAAGTGCGA ACTTACTTCC ATGAGTTTGG TCACGTCATG 
CATCAGATCT GTGCACAGAC TGACTTTGCA CGATTTAGTG GAACAAATGT GGAAACTGAC 
TTTGTAGAGG TGCCATCACA AATGCTTGAA AACTGGGTGT GGGACATTGA CTCCCTGCGA 
AAACTGTCAA AACATTATAG AGACGGACAC CCTATCACAG ACGAGCTGCT GGAGAAGCTT 
GTGGCTTCGA GACTGGTCAA CACAGGTCTT CTGACGCTTC GCCAAATTGT TTTGAGCAAA 
GTTGACCAGT CCCTCCATAC CAATGCGTCG CTGGATGCCG CGAGCGAATA CGCTAAATAC 
TGCACAGAAA TTTTGGGAGT TGCAGCTACG CCAGGCACAA ATATGCCAGC TACCTTTGGA 
CATTTGGCAG GAGGGTATGA CGGCCAGTAT TATGGATATC TTTGGAGTGA AGTCTTTTCC 
ATGGACATGT TTCACAGCTG TTTCAGAAAA GAAGGGATTA TGAATCCAGA GGTTGGAATG 
AAATACAGAA ACCTAATCCT GAAGCCTGGG GGGTCCCTGG ACGGCATGGA CATGCTCCAG 
AATTTCTTGC AACGTGAGCC AAACCAGAAA GCATTCCTGA TGAGTCGAGG CCTGAATGCT 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatic scavenger receptor BI protects against polymicrobial-induced sepsis through promoting LPS clearance in mice.
The Journal of biological chemistry289(21)14666-73(2014 May)
Guo L,Zheng Z,Ai J,Huang B,Li XA

Neurolysin knockout mice generation and initial phenotype characterization.
The Journal of biological chemistry289(22)15426-40(2014 May)
Cavalcanti DM,Castro LM,Rosa Neto JC,Seelaender M,Neves RX,Oliveira V,Forti FL,Iwai LK,Gozzo FC,Todiras M,Schadock I,Barros CC,Bader M,Ferro ES

Functional up-regulation of endopeptidase neurolysin during post-acute and early recovery phases of experimental stroke in mouse brain.
Journal of neurochemistry129(1)179-89(2014 Apr)
Rashid M,Wangler NJ,Yang L,Shah K,Arumugam TV,Abbruscato TJ,Karamyan VT

Distribution of non-AT1, non-AT2 binding of 125I-sarcosine1, isoleucine8 angiotensin II in neurolysin knockout mouse brains.
PloS one9(8)e105762(2014)
Speth RC,Carrera EJ,Bret?n C,Linares A,Gonzalez-Reiley L,Swindle JD,Santos KL,Schadock I,Bader M,Karamyan VT

Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.
Mammalian genome : official journal of the International Mammalian Genome Society24(5-6)228-39(2013 Jun)
Nakahara M,Tateyama H,Araki M,Nakagata N,Yamamura K,Araki K

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Nln cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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