Spaca3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spaca3
Entrez Gene ID	75622
Full Name	sperm acrosome associated 3
Synonyms	1700025M08Rik,ALLP17,Lyc3,SLLP1,mSLLP1
General protein information	Preferred Names sperm acrosome associated 3 Names sperm acrosome membrane-associated protein 3 acrosomal sperm protein lysozyme-like protein 3 sperm c-type lysozyme-like protein sperm lysozyme-like protein 1 precusor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_029367.1	NP_083643.1	sperm acrosome membrane-associated protein 3 precursor
XM_011249294.2	XP_011247596.1	sperm acrosome membrane-associated protein 3 isoform X1
NM_001359183.1	NP_001346112.1	sperm acrosome membrane-associated protein 3 precursor

Pathways from BioSystems

Homologies

	LOC714491	XP_001113262.1
Bos taurus (cattle)	SPACA3	NP_001098477.1
Homo sapiens (human)	SPACA3	NP_776246.1
Mus musculus (house mouse)	Spaca3	NP_083643.1
Rattus norvegicus (Norway rat)	Spaca3	NP_001099290.1
Pan troglodytes (chimpanzee)	SPACA3	XP_001174564.1
Canis lupus familiaris (dog)	SPACA3	NP_001184016.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)

Sperm Lysozyme-Like Protein 1 (SLLP1), an intra-acrosomal oolemmal-binding sperm protein, reveals filamentous organization in protein crystal form.
Zheng H, Mandal A, Shumilin IA, Chordia MD, Panneerdoss S, Herr JC, Minor W
Andrology3(4)756-71(2015 Jul)

The role of SPRASA in female fertility.
Wagner A, Holland OJ, Tong M, Shelling AN, Chamley LW
Reproductive sciences (Thousand Oaks, Calif.)22(4)452-61(2015 Apr)

Oocyte specific oolemmal SAS1B involved in sperm binding through intra-acrosomal SLLP1 during fertilization.
Sachdev M, Mandal A, Mulders S, Digilio LC, Panneerdoss S, Suryavathi V, Pires E, Klotz KL, Hermens L, Herrero MB, Flickinger CJ, van Duin M, Herr JC
Developmental biology363(1)40-51(2012 Mar)

Molecular cloning and characterization of three novel lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the c-type lysozyme/alpha-lactalbumin family.
Zhang K, Gao R, Zhang H, Cai X, Shen C, Wu C, Zhao S, Yu L
Biology of reproduction73(5)1064-71(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

distinct from c-lysozyme by substituting two essential catalytic residues (E35T/D52N), and by forming helical filaments approximately 75 A in diameter with a 25 A central pore comprised of six monomers per helix turn repeating every 33 A
Title: Sperm Lysozyme-Like Protein 1 (SLLP1), an intra-acrosomal oolemmal-binding sperm protein, reveals filamentous organization in protein crystal form.

Female mice immunized with SPRASA had profound infertility following timed matings and those mice that did become pregnant had reduced fetal viability.
Title: The role of SPRASA in female fertility.

Data show that SAS1B/SAS1R (Sperm Acrosomal SLLP1 Binding/Sperm Acrosomal SLLP1 receptor) bound to sperm acrosomal protein SlLP1 with high affinity.
Title: Oocyte specific oolemmal SAS1B involved in sperm binding through intra-acrosomal SLLP1 during fertilization.

This study demonstrates the retention of mouse sperm lysozyme-like protein (mSLLP1) in the equatorial segment of spermatozoa following the acrosome reaction and a role for mSLLP1 in sperm-egg binding and fertilization.
Title: Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg binding and fertilization.

The following Spaca3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spaca3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14485	NM_001359183.1 Latest version!	Mus musculus sperm acrosome associated 3 (Spaca3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu14485	NM_029367.1 Latest version!	Mus musculus sperm acrosome associated 3 (Spaca3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu14485	XM_011249294.2 Latest version!	Mus musculus sperm acrosome associated 3 (Spaca3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spaca3 ( NM_029367.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083643.1 Mus musculus sperm acrosome associated 3 (Spaca3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14485
Clone ID Related Accession (Same CDS sequence)	NM_029367.1 , XM_011249294.2 , NM_001359183.1
Accession Version	NM_029367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	sperm acrosome membrane-associated protein 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC100503.1. On or before Jul 24, 2006 this sequence version replaced XM_354632.4, XM_902822.2, XM_913064.2, XM_994623.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC100503.1, CA465759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCTGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGAGAT GCATGACTTC GGTCTGGATG GCTACCGGGG TTATAACCTG 
GCTGACTGGG TCTGCCTTGC TTACTACACA AGTGGCTTCA ACACAAATGC TGTGGATCAT 
GAAGCTGATG GAAGCACCAA CAATGGCATC TTCCAGATCA GCAGCCGGAG GTGGTGCAGA 
ACCCTCGCCT CGAATGGCCC CAATCTTTGC AGGATATACT GCACTGATTT GTTGAACAAT 
GATCTCAAAG ATTCTATCGT CTGTGCCATG AAGATAGTTC AAGAACCCCT GGGTCTGGGC 
TATTGGGAAG CCTGGAGGCA CCACTGCCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083643.1
CDS	213..704
Translation	MEARSRAPRRQLCPPGITWLALAYLLSCLLASSKAKVFSRCELAKEMHDFGLDGYRGYNLADWVCLAYYTSGFNTNAVDHEADGSTNNGIFQISSRRWCRTLASNGPNLCRIYCTDLLNNDLKDSIVCAMKIVQEPLGLGYWEAWRHHCQGRDLSDWVDGCDF

Target ORF information:

RefSeq Version	NM_029367.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm acrosome associated 3 (Spaca3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029367.1

ORF Insert Sequence:

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCTGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGAGAT GCATGACTTC GGTCTGGATG GCTACCGGGG TTATAACCTG 
GCTGACTGGG TCTGCCTTGC TTACTACACA AGTGGCTTCA ACACAAATGC TGTGGATCAT 
GAAGCTGATG GAAGCACCAA CAATGGCATC TTCCAGATCA GCAGCCGGAG GTGGTGCAGA 
ACCCTCGCCT CGAATGGCCC CAATCTTTGC AGGATATACT GCACTGATTT GTTGAACAAT 
GATCTCAAAG ATTCTATCGT CTGTGCCATG AAGATAGTTC AAGAACCCCT GGGTCTGGGC 
TATTGGGAAG CCTGGAGGCA CCACTGCCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spaca3 ( NM_001359183.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346112.1 Mus musculus sperm acrosome associated 3 (Spaca3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14485
Clone ID Related Accession (Same CDS sequence)	NM_029367.1 , XM_011249294.2 , NM_001359183.1
Accession Version	NM_001359183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	sperm acrosome membrane-associated protein 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645842.15. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCTGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGAGAT GCATGACTTC GGTCTGGATG GCTACCGGGG TTATAACCTG 
GCTGACTGGG TCTGCCTTGC TTACTACACA AGTGGCTTCA ACACAAATGC TGTGGATCAT 
GAAGCTGATG GAAGCACCAA CAATGGCATC TTCCAGATCA GCAGCCGGAG GTGGTGCAGA 
ACCCTCGCCT CGAATGGCCC CAATCTTTGC AGGATATACT GCACTGATTT GTTGAACAAT 
GATCTCAAAG ATTCTATCGT CTGTGCCATG AAGATAGTTC AAGAACCCCT GGGTCTGGGC 
TATTGGGAAG CCTGGAGGCA CCACTGCCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346112.1
CDS	352..843
Translation	MEARSRAPRRQLCPPGITWLALAYLLSCLLASSKAKVFSRCELAKEMHDFGLDGYRGYNLADWVCLAYYTSGFNTNAVDHEADGSTNNGIFQISSRRWCRTLASNGPNLCRIYCTDLLNNDLKDSIVCAMKIVQEPLGLGYWEAWRHHCQGRDLSDWVDGCDF

Target ORF information:

RefSeq Version	NM_001359183.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm acrosome associated 3 (Spaca3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359183.1

ORF Insert Sequence:

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCTGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGAGAT GCATGACTTC GGTCTGGATG GCTACCGGGG TTATAACCTG 
GCTGACTGGG TCTGCCTTGC TTACTACACA AGTGGCTTCA ACACAAATGC TGTGGATCAT 
GAAGCTGATG GAAGCACCAA CAATGGCATC TTCCAGATCA GCAGCCGGAG GTGGTGCAGA 
ACCCTCGCCT CGAATGGCCC CAATCTTTGC AGGATATACT GCACTGATTT GTTGAACAAT 
GATCTCAAAG ATTCTATCGT CTGTGCCATG AAGATAGTTC AAGAACCCCT GGGTCTGGGC 
TATTGGGAAG CCTGGAGGCA CCACTGCCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spaca3 ( XM_011249294.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247596.1 Mus musculus sperm acrosome associated 3 (Spaca3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu14485
Clone ID Related Accession (Same CDS sequence)	NM_029367.1 , XM_011249294.2 , NM_001359183.1
Accession Version	XM_011249294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm acrosome membrane-associated protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCTGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGAGAT GCATGACTTC GGTCTGGATG GCTACCGGGG TTATAACCTG 
GCTGACTGGG TCTGCCTTGC TTACTACACA AGTGGCTTCA ACACAAATGC TGTGGATCAT 
GAAGCTGATG GAAGCACCAA CAATGGCATC TTCCAGATCA GCAGCCGGAG GTGGTGCAGA 
ACCCTCGCCT CGAATGGCCC CAATCTTTGC AGGATATACT GCACTGATTT GTTGAACAAT 
GATCTCAAAG ATTCTATCGT CTGTGCCATG AAGATAGTTC AAGAACCCCT GGGTCTGGGC 
TATTGGGAAG CCTGGAGGCA CCACTGCCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247596.1
CDS	188..679
Translation	MEARSRAPRRQLCPPGITWLALAYLLSCLLASSKAKVFSRCELAKEMHDFGLDGYRGYNLADWVCLAYYTSGFNTNAVDHEADGSTNNGIFQISSRRWCRTLASNGPNLCRIYCTDLLNNDLKDSIVCAMKIVQEPLGLGYWEAWRHHCQGRDLSDWVDGCDF

Target ORF information:

RefSeq Version	XM_011249294.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm acrosome associated 3 (Spaca3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249294.2

ORF Insert Sequence:

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCTGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGAGAT GCATGACTTC GGTCTGGATG GCTACCGGGG TTATAACCTG 
GCTGACTGGG TCTGCCTTGC TTACTACACA AGTGGCTTCA ACACAAATGC TGTGGATCAT 
GAAGCTGATG GAAGCACCAA CAATGGCATC TTCCAGATCA GCAGCCGGAG GTGGTGCAGA 
ACCCTCGCCT CGAATGGCCC CAATCTTTGC AGGATATACT GCACTGATTT GTTGAACAAT 
GATCTCAAAG ATTCTATCGT CTGTGCCATG AAGATAGTTC AAGAACCCCT GGGTCTGGGC 
TATTGGGAAG CCTGGAGGCA CCACTGCCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)
Miyata H,Castaneda JM,Fujihara Y,Yu Z,Archambeault DR,Isotani A,Kiyozumi D,Kriseman ML,Mashiko D,Matsumura T,Matzuk RM,Mori M,Noda T,Oji A,Okabe M,Prunskaite-Hyyrylainen R,Ramirez-Solis R,Satouh Y,Zhang Q,Ikawa M,Matzuk MM

Sperm Lysozyme-Like Protein 1 (SLLP1), an intra-acrosomal oolemmal-binding sperm protein, reveals filamentous organization in protein crystal form.
Andrology3(4)756-71(2015 Jul)
Zheng H,Mandal A,Shumilin IA,Chordia MD,Panneerdoss S,Herr JC,Minor W

The role of SPRASA in female fertility.
Reproductive sciences (Thousand Oaks, Calif.)22(4)452-61(2015 Apr)
Wagner A,Holland OJ,Tong M,Shelling AN,Chamley LW

Oocyte specific oolemmal SAS1B involved in sperm binding through intra-acrosomal SLLP1 during fertilization.
Developmental biology363(1)40-51(2012 Mar)
Sachdev M,Mandal A,Mulders S,Digilio LC,Panneerdoss S,Suryavathi V,Pires E,Klotz KL,Hermens L,Herrero MB,Flickinger CJ,van Duin M,Herr JC

Molecular cloning and characterization of three novel lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the c-type lysozyme/alpha-lactalbumin family.
Biology of reproduction73(5)1064-71(2005 Nov)
Zhang K,Gao R,Zhang H,Cai X,Shen C,Wu C,Zhao S,Yu L

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Spaca3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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